PRUNE2 cDNA ORF clone, Bos taurus(cattle)

The following PRUNE2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRUNE2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa22002 XM_005210045.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22002 XM_005199190.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22003 XM_003586393.4
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22003 XM_003582534.4
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22004 XM_005210046.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22005 XM_005199191.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22005 XM_005210047.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22006 XM_005210048.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22006 XM_005199192.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22007 XM_005210049.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22007 XM_005199193.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22008 XM_005210050.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa22008 XM_005199194.3
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa237444 XM_005210045.4
Latest version!
Bos taurus prune homolog 2 (PRUNE2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91263 XM_015472512.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91263 XM_015464537.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91264 XM_015472513.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91264 XM_015464538.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91265 XM_015464539.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OBa91265 XM_015472514.1
Latest version!
Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa22002
    Clone ID Related Accession (Same CDS sequence) XM_005210045.3 , XM_005199190.3
    Accession Version XM_005210045.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210102.1
    CDS188..9433
    Translation

    Target ORF information:

    RefSeq Version XM_005210045.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210045.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22002
    Clone ID Related Accession (Same CDS sequence) XM_005210045.3 , XM_005199190.3
    Accession Version XM_005199190.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199247.2
    CDS188..9433
    Translation

    Target ORF information:

    RefSeq Version XM_005199190.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199190.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22003
    Clone ID Related Accession (Same CDS sequence) XM_003586393.4 , XM_003582534.4
    Accession Version XM_003586393.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586393.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586441.2
    CDS188..9427
    Translation

    Target ORF information:

    RefSeq Version XM_003586393.4
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586393.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22003
    Clone ID Related Accession (Same CDS sequence) XM_003586393.4 , XM_003582534.4
    Accession Version XM_003582534.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003582534.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003582582.3
    CDS188..9427
    Translation

    Target ORF information:

    RefSeq Version XM_003582534.4
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003582534.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22004
    Clone ID Related Accession (Same CDS sequence) XM_005210046.3
    Accession Version XM_005210046.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210046.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAGTACGATG AAGAAAGATC TTATAAGAGA AGTGTGAGAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT AATCTGAAGC TGAAAGAAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210103.1
    CDS188..9424
    Translation

    Target ORF information:

    RefSeq Version XM_005210046.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210046.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAGTACGATG AAGAAAGATC TTATAAGAGA AGTGTGAGAA CTAGCTGCCT TTACAATGAT
    CCAGAAATGT CTTCTATGGA GAAGGATATT AATCTGAAGC TGAAAGAAAA GCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22005
    Clone ID Related Accession (Same CDS sequence) XM_005199191.3 , XM_005210047.3
    Accession Version XM_005199191.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199191.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199248.2
    CDS188..9397
    Translation

    Target ORF information:

    RefSeq Version XM_005199191.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199191.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22005
    Clone ID Related Accession (Same CDS sequence) XM_005199191.3 , XM_005210047.3
    Accession Version XM_005210047.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9210bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210104.1
    CDS188..9397
    Translation

    Target ORF information:

    RefSeq Version XM_005210047.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210047.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AAACTAGCTG CCTTTACAAT GATCCAGAAA TGTCTTCTAT GGAGAAGGAT
    ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22006
    Clone ID Related Accession (Same CDS sequence) XM_005210048.3 , XM_005199192.3
    Accession Version XM_005210048.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210048.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGTAT TAATCTGAAG
    CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210105.1
    CDS188..9385
    Translation

    Target ORF information:

    RefSeq Version XM_005210048.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210048.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGTAT TAATCTGAAG
    CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22006
    Clone ID Related Accession (Same CDS sequence) XM_005210048.3 , XM_005199192.3
    Accession Version XM_005199192.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9198bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGTAT TAATCTGAAG
    CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199249.2
    CDS188..9385
    Translation

    Target ORF information:

    RefSeq Version XM_005199192.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199192.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGTAT TAATCTGAAG
    CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22007
    Clone ID Related Accession (Same CDS sequence) XM_005199193.3 , XM_005210049.3
    Accession Version XM_005199193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
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    301
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    481
    541
    601
    661
    721
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
    2221
    2281
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    2581
    2641
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    3181
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    7141
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    7681
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    8641
    8701
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    8821
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    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA ATATTAATCT GAAGCTGAAA GAAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199250.2
    CDS188..9349
    Translation

    Target ORF information:

    RefSeq Version XM_005199193.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA ATATTAATCT GAAGCTGAAA GAAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22007
    Clone ID Related Accession (Same CDS sequence) XM_005199193.3 , XM_005210049.3
    Accession Version XM_005210049.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210049.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA ATATTAATCT GAAGCTGAAA GAAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210106.1
    CDS188..9349
    Translation

    Target ORF information:

    RefSeq Version XM_005210049.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210049.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA ATATTAATCT GAAGCTGAAA GAAAAGCCCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22008
    Clone ID Related Accession (Same CDS sequence) XM_005199194.3 , XM_005210050.3
    Accession Version XM_005199194.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005199194.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2401
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    2521
    2581
    2641
    2701
    2761
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    3001
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    3121
    3181
    3241
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    4141
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    5881
    5941
    6001
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    6181
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    6301
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    6421
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    7141
    7201
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    7381
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    7681
    7741
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    8341
    8401
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AATATTAATC TGAAGCTGAA AGAAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005199251.2
    CDS188..9340
    Translation

    Target ORF information:

    RefSeq Version XM_005199194.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005199194.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AATATTAATC TGAAGCTGAA AGAAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22008
    Clone ID Related Accession (Same CDS sequence) XM_005199194.3 , XM_005210050.3
    Accession Version XM_005210050.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9153bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210050.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AATATTAATC TGAAGCTGAA AGAAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210107.1
    CDS188..9340
    Translation

    Target ORF information:

    RefSeq Version XM_005210050.3
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210050.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AATATTAATC TGAAGCTGAA AGAAAAGCCC TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa237444
    Clone ID Related Accession (Same CDS sequence) XM_005210045.4
    Accession Version XM_005210045.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: protein prune homolog 2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210045.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 1 indel ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACGCCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGCCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTGACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210102.2
    CDS181..9426
    Translation

    Target ORF information:

    RefSeq Version XM_005210045.4
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (PRUNE2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210045.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACGCCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGCCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTGACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AGTACGATGA AGAAAGATCT TATAAGAGAA GTGTGAGAAC TAGCTGCCTT
    TACAATGATC CAGAAATGTC TTCTATGGAG AAGGATATTA ATCTGAAGCT GAAAGAAAAG
    CCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91263
    Clone ID Related Accession (Same CDS sequence) XM_015464537.1 , XM_015472512.1
    Accession Version XM_015464537.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AACTGGATGA AGATCTGAGG GAAGCATCTG AAGCAGCTAA AACTAGCTGC
    CTTTACAATG ATCCAGAAAT GTCTTCTATG GAGAAGGATA TTAATCTGAA GCTGAAAGAA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320023.1
    CDS188..9436
    Translation

    Target ORF information:

    RefSeq Version XM_015464537.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464537.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AACTGGATGA AGATCTGAGG GAAGCATCTG AAGCAGCTAA AACTAGCTGC
    CTTTACAATG ATCCAGAAAT GTCTTCTATG GAGAAGGATA TTAATCTGAA GCTGAAAGAA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91263
    Clone ID Related Accession (Same CDS sequence) XM_015464537.1 , XM_015472512.1
    Accession Version XM_015472512.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AACTGGATGA AGATCTGAGG GAAGCATCTG AAGCAGCTAA AACTAGCTGC
    CTTTACAATG ATCCAGAAAT GTCTTCTATG GAGAAGGATA TTAATCTGAA GCTGAAAGAA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327998.1
    CDS188..9436
    Translation

    Target ORF information:

    RefSeq Version XM_015472512.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472512.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAACC GCCTAATTCT
    CTGGATCTTG ATAGCACTCA TCCTCGGAAA ATCAAACTCA CAGCCCCAAA TATCAATCTG
    TCTCTGGATC AAAGTGAAGG GTCTGTTCTC TCTGATGATA ACTTGGACAG TCCAGATGAG
    ATTGATATCA ATGTGGATGA ACTTGAGACT CCCGATGAAG CAGATTCTTT TGAGTACACT
    GGCCATGAGG ATTCCACAGG CAACAAAGAT TCTGGCCAAG AGTCAGAGTC TATTCCAGAA
    TATACGGCAG AAGAGGAGCG GGAGGACAAC CGGCTGTGGA GGACCGTGGT GATCGGAGAA
    CAGGAGCAGC GGATTGACAT GAAGGTCATC GAGCCCTATA GGAGAGTCAT TTCTCACGGA
    GGAGACTCAG GATACTATGG GGATGGCCTA AATGCCATCA TTGTGTTTGC CGCCTGTTTT
    CTGCCAGACA GCAGTCGGGC GGACTACCAC TATGTCATGG AAAATCTTTT CCTATATGTA
    ATAAGTACTT TAGAGTTGAT GGTAGCTGAA GACTATATGA TTGTGTACTT GAATGGTGCA
    ACCCCAAGAC GGAAGATGCC AGGGTTAGGC TGGATGAAGA AATGTTACCA GATGATTGAC
    AGACGGTTGA GGAAGAATTT AAAATCATTC ATCATTGTTC ATCCATCTTG GTTCATCCGA
    ACAATCCTTG CAGTTACACG ACCTTTTATA AGTTCCAAAT TCAGCAGTAA AATTAAATAC
    GTCAGTAGTT TAGCAGAACT CAGTGGACTG ATCCCAATGG ATTGCATCCA TATTCCAGAG
    AGCATCATCA AACTGGATGA AGATCTGAGG GAAGCATCTG AAGCAGCTAA AACTAGCTGC
    CTTTACAATG ATCCAGAAAT GTCTTCTATG GAGAAGGATA TTAATCTGAA GCTGAAAGAA
    AAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91264
    Clone ID Related Accession (Same CDS sequence) XM_015472513.1 , XM_015464538.1
    Accession Version XM_015472513.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGTGTGATT CTTACAGAGA TTTAACTAAT CGAAGCAAAC GTTTGGAGAA GGTCCATGTG 
    GTTATTGGAC ACAAATCCTG TGATTTGGAT TCTCTCATTT CTGCCTTCAC ATATGCTTAC
    TTTCTAGACA AGGTCAGCCC ACCAGGGGTT CTCTGTTTGC CCGTGCTGAA CATACCAAGA
    ACTGAATTCA ATTACTTCAC TGAAACAAGG TTTATTTTAG AAGAGCTAAA TATCTCTGAA
    TCATCCCACA TATTCCGAGA TGAAATTAAT CTGCATCAGC TAAATAATGA AGGGAAGTTA
    TCTCTAACAC TTGTTGGCAG CAACGTACTG GCAAGTGAGG ACAAAACTTT AGAATCAGCA
    GTTGTCAAGG TCATTAATCC GGTGGAGCAG GGCGACACCG GCTTAGAGTT CCGAGAGTCT
    TCGTCGTCTC TGGTGGTGAA AGAGATCCTC CAGGAGGCTC CCGAGCTCAT CACCGAGCAG
    CTGGCTCATC TGCTCAGAGG TAGCATCCTT TTTAAGTGGA TGGCCATGGA GCCCAAGAAG
    ATCTCAGAAA AGCAGGAGGA AATTCTTTCT ATCCTGGAAG AAAAATTTCC CAGCTTACCT
    CCAAGAGAGG ACATTATCAA CATCCTGCAG GAGACCCAGT CCAGTGCTCA GGGTTTAGGT
    ATTGAACAGA CAATGCTGAA GAATCTAAAG GAACTTTCAG ATGGAGAAAT AAAAGTGGCC
    ATTAGCACTG TGAACATGAC CCTGGAGAAC TGTACATTTC ACAGCAATAT CACCAGTGAT
    TTGAAAGCAT TCACAGAAAA GTTTGGTTTT GATGTCCTCA TCCTCCTGGC CAGTTACCTG
    TCAGAGGAGC AGCAGTCCAG ACAACAGATC GCTGTGTATT CTGAGAACCT AGAGCTGTGC
    AGTCAGATCT GCTGTGAACT GGAAGAGTGC CAGAACCCTT GCCTGGAGCT GGAACCCTTT
    GAGTGTGGCT GTGATGAGAT CCTGGTGTAC CAGCAGGAGA ACCCTTCAGT GACTTGTGAT
    CAGGTGGTTC TCCTTGTCAA GGAAGTCATC AACAGGAGGT GTCCGGAGAT GGCCTCCAAC
    AGCCGGACAT CCTCCACAGA AGCGGTGGCA GGCAGTGCCC CCCTCTCCCA GGGATCTTCA
    GGGATTATGG AGTTGTATGG TTCTGATGTA GAGCCACAGC CCAGCTCCGT GAATTTCTTG
    GAAAATCCTC CTGATCTCGG CGATTCTAAC CAGGCCCAGG TGGATGTCAA TGCGGACCTG
    GTGAGCCCAG ACAGTGGGCT GGCCACCATC AGGAGCAGCC GCTCATCCAA GGAGAGCTCC
    GTGTTCCTCA GTGACGACAG CCCTGTGGGA GAAGGGGCGG GGCCTCACCA CAGCCTCCTC
    CCAGGGTTTG ACTCCTACAG TCCCATCCCC GAAGGAGCAG TAGCGGAGGA GCATGCCCGC
    TCTGGAGAAC ATGGTCAATC ATTCGACCTT TTCAACTTTG ACCCAGCACC CAGGGTTTCT
    GGGCAGTCTC AACCATCCTC CCGTTCTGCA GACTACTCCC CAACAGATGA CTTCTTCCCC
    AACAGCGATT CCTCAGAAGG ACAGCTCACC ATGGGGTCTA AAGAACTTGA CAGGAGGGGG
    ATGGACATGC CTAATTATTC ATCCAGTTCT CTCTTGTCAG GGGCTGGCAA AGAGAGCCTC
    GTGGAATTTG ATGAGGAGTT TGTCCAGAGA CAAGAAAGTC CCGGGGATAA TTCTGAAAGA
    AATTTGAGCC TGACAGGTTT TATGCAAGAT GAATCTCCTT CTCCAGAAAG TCTAAAAACT
    GTTGGAAAGA GGATCCCACC AACACCTATG AACAGCTTTG TGGAAAGTTC ACCATCCACT
    GAAGAACCAG CTCTGCTCTG TTCAGAAGAC GTGACCCCCA AGACAGTCAA CACAGGCCAC
    CCAGGGCCAC CTCTAACCCG GGCGCGATGC AGCAGCTGGT GGGATGGTTT GGAAATCGAC
    TCTAAAAACA TCGTGGATGC ATGGAGTTCC AGCAAACAGG AATCTGTCTT CCAGAGCCCT
    GAGTCCTGGA AAGATCATAA GCCAAGCCCA GCTGATAGAA GAGCCTCAGA CTCTATATTT
    CAACCCAAGA ATCTCGACTT CCCAAAGTCA AGTCCCTGGG AGTCAGAATT TGGTCAATCT
    GGGCTGGGCA ACAAGAATAT TCAAGACTCA GATGAGAAAA ACTTCCAGTT TCAGGACATG
    CCCCCTGAGA AATCACGTTT GCTGAATGTT TCTCCTCACG GAACAAACCA CCTGATAGAA
    GACTTCGCTG CCCTGTGGCG TTCTGACCGC TCTCCCACAG CCATGCCTGA GCCGTGGGGA
    AACCCCACAG AGGATGAGGA ACCAGCTGCT GCAGCGTCAT TCCCAGCCTG GAGTGCATTT
    GGTGAAGAAG GTGATGCCAA AGCTTTGAAA AATACTTGGA ATCTGCACCC AACGAGCAAC
    GAGACACCTT CTGTGAGAGA CCCAAACGAG TGGGCCATGG CCAAAAGTGG GATTGCTTTC
    CCTTCAGAAG ACCTCGTGGA CAGTTCACCC AGTGAGGCAA ATAATGAAGC GGTTCAAAAA
    ATCTGGGGCA AGAAGAACCA TGACCCCAGG GATGATATTC TCATATCTGG AAATCCCACA
    TCTGATCTGG ATCATCCATG GAATAGTTCT AAGCCAGCAA AGGACCATCA GAACAGTGTG
    GTGGACCCAA CAATTAGGGG CAAAATGTAC AAAAATGTAG ATTCTTGGAA TCTTTTCGAG
    GAGAGTAACA AGGAAGGAGG TTCAGATGTC CCAGCACCTT GGGAGGACTC CTTCTTATCC
    TATAAATGTT CTGACTACAG TGCATCCAAC GTCAGGGAAG ACTCAATGCC CTCCCCCTTA
    GATACCAACT ACTCCACCTC TGACTCTTAT ACTTCACCAA CATTGGCCAA AGAGGAGAAA
    GAAACCAAAG ACAAGCCACT CACTAAAGAG GAGGGTTTTG AGTCACAAGA TGCTAATTGT
    ACTGCAGGGG GCGCTGACAT GCCTCCTCAG TCCCCGCAGC AGCCACCAAG AAATCGAATG
    AGTTCAGGTC CTGGGAACCT GGAAATGTGG GCTTCATCTC AGGCAGATGG TGGCACAGAA
    ATAAATGCCA CCCATAGCCC AGATAAAGAT CTACTCCAAG TAGAGCCCAC GGATGATCAG
    AATGTCTCCT TTGAGGATGA CATGGGGGAA AGCAGCCAGT CCAGTTATGA CGACCCCAGC
    ATGATGCAGC TGTACAACGA GACGAACCGA CAGCTCACAC TTCTGCACAG CAGTGTTCAC
    GCCCGGCAGG CAGCCCCTGA CAGCCTCGAC ACGTGGAACA GAGTGATTCT GGAGGACACT
    CAGTCTACTG CAACCATCTC GGATATGGAC AATGACTTGG ACTGGGATGA CTGCAGTGCG
    GGCGTGGCCA TCACTGGTGA AGGTCAGGCA CAAGGGTACA TGGCGGAAGG TAGCGAACCT
    GAAACCCGAT TTTCAGTGAG ACAGGTAGAA CCCTGGGGCG TGGAGTATCA GGAGGCAAAT
    CAGTCAGACT GGGAACTCCC TGCCTCTTCT GAGCACAGCC TGGACGTGGC TCCTGATGAA
    TACCACGTGC TGAATGAGAA AAGTGGGCAG CTGATCGCGA ATAGTATTTG GGATTCTGTC
    ATGAGAGATA ATGCCATGTC ATCATTAATA TTACCTAGCC CATCATATGC TACAGATACA
    GAACAGAGCA GATCACCTCC TGAAACAACC AGCTCATTAG AAAAAATTCA GGATAGTCAC
    ATTGCAGACG TGCTGGAAGC CTCTGAAGCC AGTGAGTCTG AAACAATCGA TCCTGACAAG
    CAGGACACAT GCAGAGGAAC CCTGCCAAGT GATTCGGCAT ATTTGGTGAC CAGACTTGAG
    AATCCAGAGC ATTTTGCATG CTCAGATCCC TGGAAGGGTC CGAGCTATGG ACAAAAGGAA
    AGTGAGAAGG AATTCCACGG AGCTGCTGAT AGGGAGAATT CCAGTGATGA GCTAATGGAT
    GGAACCTCAG AACCTTCTGG AAAAGGGCCA GGTAGCCAGG AATGCCCATC CCCAGCTGCA
    TCTGATCATC AAGAAATCTC CAATGAACCA GGCAAAATCA GAAATTTTTC AGTGACTTCC
    AGTCCTCAGA CCAAGGAACC CCAGGAAGTA TTAGAGCAGG AGAAGGGGTC TTACACTCCA
    GACCCCTGTG ATGTGCAAAC AGAGGTGTCC GAAGATCACT TGCCAGCAAG AGAGACCTGT
    GAAGAGCTCC TCATAAGCCC CAGAAGAAAT TCAAGTGAAA CTACTGAAAT TCCAGGTAGG
    CTGAATTCCA CAAAAAGTGT GTGTCTACCT CAAGTGGATG TGGAGAAAGC TGAAGAATGT
    GACATTCTGG AGCCAGAGAA AATTGACCAA GAGCCACCTG ATGAATGTCC CCAAGGCCTT
    GACTCTTGGG ATGGCCCTAA CGACAGTGAT GTGCTGGTCA GTGGTGCAAG TTCTGAGATA
    ACTATGAATC TCGAAGTTAG AAATACTGAA AACAGCCCTC CAAGAGCCAA TTTATCAGGA
    AATGATGACA GAGATAGTAT TTCTAGTGAG TATACACTTT CAAGTGCATC TAGTCCTGAC
    ATAAATGATT CTTCAGCTAC ATTGCCTTCC TGGGACCAAG GACTCAATAC TGGGCATCAG
    AAGGAAAATC AAAATGATTG GAGTACACAG AATCGCCAAG AATCTGAACT AATGACTACT
    GATGGTCATA CAGAAGTAGT TGCTGAAATG CAGGGTTTGG AGAAAAACAG AATGGACAGG
    TTTGAAAGGA GCTTAGATCA CAAGGTTCCT AAATTTTTAG AGTTTTGGGG TGACTCAATA
    GATGGTGATT CCTTTTCTTC TTTATCCAGC CCAGAAACAG GTAGATATTC TGAATATTCA
    GATGCGTATC AGGAAAGAAA GCTAGTGGTC AGCCATCAGG AGAAAAATGA ACGTGATCTT
    CCTGAAACTG TACAGCCAGA GGACACCAGC TTCATTTCAA CAAGCTCAGG TTCTGATGAT
    GGTGAAGGCT ATGAAGAGCC AGTGGAGAAA GAGATCCATT CGGATACTTG TCAAGTGGCT
    CCGAGTGAAC TCAGAGCTTG GGATTCATTG AATAAATCTA ATAAGTCCTT GGCCACAGCA
    GATCCTGAAT CTGAGCAATG TTCTGTCTAT AGAGGCAGTC CAGAGCAAGC AGAGAATGAG
    AATAAGTCAA ACCCATCCTG TGATGATCAA CAAAGCAGCC CTGATCACTG GAATTTCTCA
    CCAGTAAGGA AGACTGAATT ACAGGTAACA TCAGTCGATA AGGAGACAAG GTCTTCTCCA
    GAAATAGGGA GGACAGGAGA TATCATATGG CAAATATCTC CCAAAGCTGA ACCAAATAAT
    GAAAATTACT CTAAATTGGA AATGGCTGAC TTCTCAACTG AGAGCACGGA GTGGTGGAAG
    CCCTCCCTAC AGGAAGGACG ACCAATCGAG AGTGCATTTG AAGGAGAATT ATCTAACTCA
    AGTGCTGTGC TAGAAATGAA TTCCTCAGTG TGCGAAAACA TGGGTCCTTG GGGAGTGCCC
    ATTCAGGGTG ATACTGAATC CCTGGCACTA CATGGTACCA ATCCTTTCAA TGATAACCAT
    CAGTCACCCT TTCTAGATCA TAATGGGGAG AATGCCCACG AGCAGCTTTG GAACATTCAA
    CCAAGGCAGC CAGACCCAGA AGCTGATCAG CTTAACCAAC TTGTAATATT GGAACAAATT
    AAAGACACAG ATTCCAGAGA ACAAACCTTC ATGTCTTCTG CCGGTGACGA ACTCTCCTCT
    GAAGTGCCTA CGCAAGAACA GTGTCAGAAT TCAGTGCTGT CTGCCTGGGA CCAGCCAGAC
    TCGTCTTGTA CTTACGCAGA TGAAAATGGA TGCATTGTAT CTGGTGTAGC AACAACTGAG
    TGTCAAGAAA CAAATTGGTG GGAAGAAGAA AAATCACACC CAAGTGAAAT GACAAATTCA
    AACATGACCT CTGAAGATTT CCCTGCTGTC AGTTCTCCCA CCCAGATGAT GAACAAGTCA
    GGCTCTGAAT GGAGTAACTC TACCCCTACT GAGGGCCCCC AGGGCACGTT TGTTCCAGAT
    ATTTTACACG GCAACTTCCA AGAGGGTGGG CACCTGGCCT CATCTTCGCC CGACTTGTGG
    ATGGGTGTGA AACAGCCCTT CAGTGTGAAG ACAGATGGTG AGAATCCTGA TATACTGACT
    CACTGTGACC ACGACAGCAA TTCCAAGGCT TCCAACAGCC CTGATGTTTG TCATGATTAT
    GAAGTAAAGC CAGAGACTAA GCAGCACATC GATGCTGGGC TGGGACCTGA AAGGGAAGCC
    AGTGAGTTAT ATCTCACTGA GCCAGAGATA GATGAAGAAT CCAGACAGGA GCCTGGGCAG
    GAATTTGTCC CTTATACTTC AGAACTGTCC TCTGAAAATG TCATTCCACT GCCTCCAGTC
    AGCGAGCAGA CAAACACAAA TGACTCATCT CAGCCTTCCT CTCACGAGTG TTTTCCTGAA
    CCTTCTGAAA TTAATGGTGA AAACAATGCA GGCTTAAAAG CATCAGAACA AGGAGCCAAC
    CCAGATATGG CACCAGTTTT GGAACCTGTT GACAGAACAA TCTCAATGAT TGAAAATGTG
    GAAACTGGTA TTTGTGTAAC TTACCAAGAG ACAACTCTGG ACGACAGTAG ATCTTCGATA
    TCAGAAGACT TTCTTCCTCA GAGGCAGACC GATGCAGGGG ACTTGTTGGA CCATGAGCAG
    CCCTCTGTTA CTGAGAATCC TGCCACGGTT CCTGATGCTT TGCTAGCCTC GGACACTTGT
    CTGGATGTCA GTGAAGCTGC CTTCGACCAC AGTCTCAGCG ATGCCTCGGG TCTCAACACA
    TCCACGGGAA CAATAGATGA CATGAGTAAG TTGACATTAT CAGAAGGCAT TCCTGAAACG
    CCATTTGATG GGGAGACAGG GAAGCATGAT ATCTGTTCCT CTGAAGCCTC GTGGGGTGAC
    TTTGAGTATG ATGTCATGGG CCAAAATATT GATGAAGACT TAATGAAAGA GCCTGAACAC
    TTTGTTTATG GTGTTGACCC TCCCTTGGAA GAAGATGCCC TGAAGCATGA GTTGGCACCT
    TACACACCTC CCTTTGATTT ATCCTATCTC ACGGAACCTA CTCCTGGCAT CAAAACCACA
    GAGGAATCTG GGACTCCAGA GGATGAGTCT CTGGGGAGCG AAGCAGCAGA GATACTGCTT
    TCTGCCCTTC CTGATGAAAG AAACCAGGAA AACCATGCTG AGACCACAGA CAGACAGCCC
    GAAGGCCAGC CAGTTGTACT GCACATTCAC GAAGACCCTG AGTCTGTTCC TTTGCCTGTC
    AGAATTGATG CCAACCCTGA GTTGTCTCCA TCACACATTG ACTGGGAAGT AGAAACAGAT
    CGTTCAGATT CACCAGCAGG TGGAGACATA GGACCACCAA GTGGTGCCAG TGAGGGAATA
    TTAGAGTTGG AAGAAGAAAA AATAATTCCT ACCAAAGAGC CTGAGCAGGT AAAATCAGAA
    CATGGGGAGG AAAGGAACAC TGAGAAGAAA GAAGATCGTC ATGCATTGCA CATGGATTAC
    ATACTTGTAA ACCATGAAGA AAATTCACTG CCAGAGCCAG AGGCCTGTGA AGCTAGAGAA
    ATCACATTGG AATTAGAAGA GCTTCACGTA GATTCCGAGG AAAAAGGGCT GCCAGAAACT
    CAGTTAGCCA GCTCCCCGGA CACAGGTCAA CCAGCTTCCT TAAATGAAAG AAATCATCTA
    TCTACAGAAA GAATGTCTTC CAAAGATGAT AAGAGATCAT CTTCTGAAAG CCCTGGGCAA
    GACCAGAGTT GGATGGTCTT GGGCCGTAGT GAGGTTAGTG ATCCATCATC AGAGACCATG
    GACTCAGGAC CTGGATGGTC TGGTGAGGCT GTGGAGCCAG CCTCTGATCA CAGCTTGGGC
    CAGAGCCCCC AAATACAGAT TCTGGAAGAA ATGAAGCCTC TAGAATCTTT AGCACTAGGG
    GAAGCCTCTG ATCTGGGCAG CCAATCACGG AGGAGCAAGA GCCGAGGCAA AGCTGGCCCT
    GATGCAGTTA TGTTGCAGCC TGTCACCCAT GACAATGAAT GGGAAATGCT TTCACCACAA
    AACATAATCC CTGAAATGGA AATGGAGGAG GAGACAGAGT TTCTTGAGCC CAGAACAAGG
    AAACCAAGAC GGAATGGACT GCTGTCAGAG GATGTGGGAA TGGACATCCA CTCTGAGGAG
    GGGATGCTGA GTCCCATTGC TGCAGACATG AGGCCAGAAC CGCCTAATTC TCTGGATCTT
    GATAGCACTC ATCCTCGGAA AATCAAACTC ACAGCCCCAA ATATCAATCT GTCTCTGGAT
    CAAAGTGAAG GGTCTGTTCT CTCTGATGAT AACTTGGACA GTCCAGATGA GATTGATATC
    AATGTGGATG AACTTGAGAC TCCCGATGAA GCAGATTCTT TTGAGTACAC TGGCCATGAG
    GATTCCACAG GCAACAAAGA TTCTGGCCAA GAGTCAGAGT CTATTCCAGA ATATACGGCA
    GAAGAGGAGC GGGAGGACAA CCGGCTGTGG AGGACCGTGG TGATCGGAGA ACAGGAGCAG
    CGGATTGACA TGAAGGTCAT CGAGCCCTAT AGGAGAGTCA TTTCTCACGG AGGAGACTCA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327999.1
    CDS41..9280
    Translation

    Target ORF information:

    RefSeq Version XM_015472513.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472513.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGTGTGATT CTTACAGAGA TTTAACTAAT CGAAGCAAAC GTTTGGAGAA GGTCCATGTG 
    GTTATTGGAC ACAAATCCTG TGATTTGGAT TCTCTCATTT CTGCCTTCAC ATATGCTTAC
    TTTCTAGACA AGGTCAGCCC ACCAGGGGTT CTCTGTTTGC CCGTGCTGAA CATACCAAGA
    ACTGAATTCA ATTACTTCAC TGAAACAAGG TTTATTTTAG AAGAGCTAAA TATCTCTGAA
    TCATCCCACA TATTCCGAGA TGAAATTAAT CTGCATCAGC TAAATAATGA AGGGAAGTTA
    TCTCTAACAC TTGTTGGCAG CAACGTACTG GCAAGTGAGG ACAAAACTTT AGAATCAGCA
    GTTGTCAAGG TCATTAATCC GGTGGAGCAG GGCGACACCG GCTTAGAGTT CCGAGAGTCT
    TCGTCGTCTC TGGTGGTGAA AGAGATCCTC CAGGAGGCTC CCGAGCTCAT CACCGAGCAG
    CTGGCTCATC TGCTCAGAGG TAGCATCCTT TTTAAGTGGA TGGCCATGGA GCCCAAGAAG
    ATCTCAGAAA AGCAGGAGGA AATTCTTTCT ATCCTGGAAG AAAAATTTCC CAGCTTACCT
    CCAAGAGAGG ACATTATCAA CATCCTGCAG GAGACCCAGT CCAGTGCTCA GGGTTTAGGT
    ATTGAACAGA CAATGCTGAA GAATCTAAAG GAACTTTCAG ATGGAGAAAT AAAAGTGGCC
    ATTAGCACTG TGAACATGAC CCTGGAGAAC TGTACATTTC ACAGCAATAT CACCAGTGAT
    TTGAAAGCAT TCACAGAAAA GTTTGGTTTT GATGTCCTCA TCCTCCTGGC CAGTTACCTG
    TCAGAGGAGC AGCAGTCCAG ACAACAGATC GCTGTGTATT CTGAGAACCT AGAGCTGTGC
    AGTCAGATCT GCTGTGAACT GGAAGAGTGC CAGAACCCTT GCCTGGAGCT GGAACCCTTT
    GAGTGTGGCT GTGATGAGAT CCTGGTGTAC CAGCAGGAGA ACCCTTCAGT GACTTGTGAT
    CAGGTGGTTC TCCTTGTCAA GGAAGTCATC AACAGGAGGT GTCCGGAGAT GGCCTCCAAC
    AGCCGGACAT CCTCCACAGA AGCGGTGGCA GGCAGTGCCC CCCTCTCCCA GGGATCTTCA
    GGGATTATGG AGTTGTATGG TTCTGATGTA GAGCCACAGC CCAGCTCCGT GAATTTCTTG
    GAAAATCCTC CTGATCTCGG CGATTCTAAC CAGGCCCAGG TGGATGTCAA TGCGGACCTG
    GTGAGCCCAG ACAGTGGGCT GGCCACCATC AGGAGCAGCC GCTCATCCAA GGAGAGCTCC
    GTGTTCCTCA GTGACGACAG CCCTGTGGGA GAAGGGGCGG GGCCTCACCA CAGCCTCCTC
    CCAGGGTTTG ACTCCTACAG TCCCATCCCC GAAGGAGCAG TAGCGGAGGA GCATGCCCGC
    TCTGGAGAAC ATGGTCAATC ATTCGACCTT TTCAACTTTG ACCCAGCACC CAGGGTTTCT
    GGGCAGTCTC AACCATCCTC CCGTTCTGCA GACTACTCCC CAACAGATGA CTTCTTCCCC
    AACAGCGATT CCTCAGAAGG ACAGCTCACC ATGGGGTCTA AAGAACTTGA CAGGAGGGGG
    ATGGACATGC CTAATTATTC ATCCAGTTCT CTCTTGTCAG GGGCTGGCAA AGAGAGCCTC
    GTGGAATTTG ATGAGGAGTT TGTCCAGAGA CAAGAAAGTC CCGGGGATAA TTCTGAAAGA
    AATTTGAGCC TGACAGGTTT TATGCAAGAT GAATCTCCTT CTCCAGAAAG TCTAAAAACT
    GTTGGAAAGA GGATCCCACC AACACCTATG AACAGCTTTG TGGAAAGTTC ACCATCCACT
    GAAGAACCAG CTCTGCTCTG TTCAGAAGAC GTGACCCCCA AGACAGTCAA CACAGGCCAC
    CCAGGGCCAC CTCTAACCCG GGCGCGATGC AGCAGCTGGT GGGATGGTTT GGAAATCGAC
    TCTAAAAACA TCGTGGATGC ATGGAGTTCC AGCAAACAGG AATCTGTCTT CCAGAGCCCT
    GAGTCCTGGA AAGATCATAA GCCAAGCCCA GCTGATAGAA GAGCCTCAGA CTCTATATTT
    CAACCCAAGA ATCTCGACTT CCCAAAGTCA AGTCCCTGGG AGTCAGAATT TGGTCAATCT
    GGGCTGGGCA ACAAGAATAT TCAAGACTCA GATGAGAAAA ACTTCCAGTT TCAGGACATG
    CCCCCTGAGA AATCACGTTT GCTGAATGTT TCTCCTCACG GAACAAACCA CCTGATAGAA
    GACTTCGCTG CCCTGTGGCG TTCTGACCGC TCTCCCACAG CCATGCCTGA GCCGTGGGGA
    AACCCCACAG AGGATGAGGA ACCAGCTGCT GCAGCGTCAT TCCCAGCCTG GAGTGCATTT
    GGTGAAGAAG GTGATGCCAA AGCTTTGAAA AATACTTGGA ATCTGCACCC AACGAGCAAC
    GAGACACCTT CTGTGAGAGA CCCAAACGAG TGGGCCATGG CCAAAAGTGG GATTGCTTTC
    CCTTCAGAAG ACCTCGTGGA CAGTTCACCC AGTGAGGCAA ATAATGAAGC GGTTCAAAAA
    ATCTGGGGCA AGAAGAACCA TGACCCCAGG GATGATATTC TCATATCTGG AAATCCCACA
    TCTGATCTGG ATCATCCATG GAATAGTTCT AAGCCAGCAA AGGACCATCA GAACAGTGTG
    GTGGACCCAA CAATTAGGGG CAAAATGTAC AAAAATGTAG ATTCTTGGAA TCTTTTCGAG
    GAGAGTAACA AGGAAGGAGG TTCAGATGTC CCAGCACCTT GGGAGGACTC CTTCTTATCC
    TATAAATGTT CTGACTACAG TGCATCCAAC GTCAGGGAAG ACTCAATGCC CTCCCCCTTA
    GATACCAACT ACTCCACCTC TGACTCTTAT ACTTCACCAA CATTGGCCAA AGAGGAGAAA
    GAAACCAAAG ACAAGCCACT CACTAAAGAG GAGGGTTTTG AGTCACAAGA TGCTAATTGT
    ACTGCAGGGG GCGCTGACAT GCCTCCTCAG TCCCCGCAGC AGCCACCAAG AAATCGAATG
    AGTTCAGGTC CTGGGAACCT GGAAATGTGG GCTTCATCTC AGGCAGATGG TGGCACAGAA
    ATAAATGCCA CCCATAGCCC AGATAAAGAT CTACTCCAAG TAGAGCCCAC GGATGATCAG
    AATGTCTCCT TTGAGGATGA CATGGGGGAA AGCAGCCAGT CCAGTTATGA CGACCCCAGC
    ATGATGCAGC TGTACAACGA GACGAACCGA CAGCTCACAC TTCTGCACAG CAGTGTTCAC
    GCCCGGCAGG CAGCCCCTGA CAGCCTCGAC ACGTGGAACA GAGTGATTCT GGAGGACACT
    CAGTCTACTG CAACCATCTC GGATATGGAC AATGACTTGG ACTGGGATGA CTGCAGTGCG
    GGCGTGGCCA TCACTGGTGA AGGTCAGGCA CAAGGGTACA TGGCGGAAGG TAGCGAACCT
    GAAACCCGAT TTTCAGTGAG ACAGGTAGAA CCCTGGGGCG TGGAGTATCA GGAGGCAAAT
    CAGTCAGACT GGGAACTCCC TGCCTCTTCT GAGCACAGCC TGGACGTGGC TCCTGATGAA
    TACCACGTGC TGAATGAGAA AAGTGGGCAG CTGATCGCGA ATAGTATTTG GGATTCTGTC
    ATGAGAGATA ATGCCATGTC ATCATTAATA TTACCTAGCC CATCATATGC TACAGATACA
    GAACAGAGCA GATCACCTCC TGAAACAACC AGCTCATTAG AAAAAATTCA GGATAGTCAC
    ATTGCAGACG TGCTGGAAGC CTCTGAAGCC AGTGAGTCTG AAACAATCGA TCCTGACAAG
    CAGGACACAT GCAGAGGAAC CCTGCCAAGT GATTCGGCAT ATTTGGTGAC CAGACTTGAG
    AATCCAGAGC ATTTTGCATG CTCAGATCCC TGGAAGGGTC CGAGCTATGG ACAAAAGGAA
    AGTGAGAAGG AATTCCACGG AGCTGCTGAT AGGGAGAATT CCAGTGATGA GCTAATGGAT
    GGAACCTCAG AACCTTCTGG AAAAGGGCCA GGTAGCCAGG AATGCCCATC CCCAGCTGCA
    TCTGATCATC AAGAAATCTC CAATGAACCA GGCAAAATCA GAAATTTTTC AGTGACTTCC
    AGTCCTCAGA CCAAGGAACC CCAGGAAGTA TTAGAGCAGG AGAAGGGGTC TTACACTCCA
    GACCCCTGTG ATGTGCAAAC AGAGGTGTCC GAAGATCACT TGCCAGCAAG AGAGACCTGT
    GAAGAGCTCC TCATAAGCCC CAGAAGAAAT TCAAGTGAAA CTACTGAAAT TCCAGGTAGG
    CTGAATTCCA CAAAAAGTGT GTGTCTACCT CAAGTGGATG TGGAGAAAGC TGAAGAATGT
    GACATTCTGG AGCCAGAGAA AATTGACCAA GAGCCACCTG ATGAATGTCC CCAAGGCCTT
    GACTCTTGGG ATGGCCCTAA CGACAGTGAT GTGCTGGTCA GTGGTGCAAG TTCTGAGATA
    ACTATGAATC TCGAAGTTAG AAATACTGAA AACAGCCCTC CAAGAGCCAA TTTATCAGGA
    AATGATGACA GAGATAGTAT TTCTAGTGAG TATACACTTT CAAGTGCATC TAGTCCTGAC
    ATAAATGATT CTTCAGCTAC ATTGCCTTCC TGGGACCAAG GACTCAATAC TGGGCATCAG
    AAGGAAAATC AAAATGATTG GAGTACACAG AATCGCCAAG AATCTGAACT AATGACTACT
    GATGGTCATA CAGAAGTAGT TGCTGAAATG CAGGGTTTGG AGAAAAACAG AATGGACAGG
    TTTGAAAGGA GCTTAGATCA CAAGGTTCCT AAATTTTTAG AGTTTTGGGG TGACTCAATA
    GATGGTGATT CCTTTTCTTC TTTATCCAGC CCAGAAACAG GTAGATATTC TGAATATTCA
    GATGCGTATC AGGAAAGAAA GCTAGTGGTC AGCCATCAGG AGAAAAATGA ACGTGATCTT
    CCTGAAACTG TACAGCCAGA GGACACCAGC TTCATTTCAA CAAGCTCAGG TTCTGATGAT
    GGTGAAGGCT ATGAAGAGCC AGTGGAGAAA GAGATCCATT CGGATACTTG TCAAGTGGCT
    CCGAGTGAAC TCAGAGCTTG GGATTCATTG AATAAATCTA ATAAGTCCTT GGCCACAGCA
    GATCCTGAAT CTGAGCAATG TTCTGTCTAT AGAGGCAGTC CAGAGCAAGC AGAGAATGAG
    AATAAGTCAA ACCCATCCTG TGATGATCAA CAAAGCAGCC CTGATCACTG GAATTTCTCA
    CCAGTAAGGA AGACTGAATT ACAGGTAACA TCAGTCGATA AGGAGACAAG GTCTTCTCCA
    GAAATAGGGA GGACAGGAGA TATCATATGG CAAATATCTC CCAAAGCTGA ACCAAATAAT
    GAAAATTACT CTAAATTGGA AATGGCTGAC TTCTCAACTG AGAGCACGGA GTGGTGGAAG
    CCCTCCCTAC AGGAAGGACG ACCAATCGAG AGTGCATTTG AAGGAGAATT ATCTAACTCA
    AGTGCTGTGC TAGAAATGAA TTCCTCAGTG TGCGAAAACA TGGGTCCTTG GGGAGTGCCC
    ATTCAGGGTG ATACTGAATC CCTGGCACTA CATGGTACCA ATCCTTTCAA TGATAACCAT
    CAGTCACCCT TTCTAGATCA TAATGGGGAG AATGCCCACG AGCAGCTTTG GAACATTCAA
    CCAAGGCAGC CAGACCCAGA AGCTGATCAG CTTAACCAAC TTGTAATATT GGAACAAATT
    AAAGACACAG ATTCCAGAGA ACAAACCTTC ATGTCTTCTG CCGGTGACGA ACTCTCCTCT
    GAAGTGCCTA CGCAAGAACA GTGTCAGAAT TCAGTGCTGT CTGCCTGGGA CCAGCCAGAC
    TCGTCTTGTA CTTACGCAGA TGAAAATGGA TGCATTGTAT CTGGTGTAGC AACAACTGAG
    TGTCAAGAAA CAAATTGGTG GGAAGAAGAA AAATCACACC CAAGTGAAAT GACAAATTCA
    AACATGACCT CTGAAGATTT CCCTGCTGTC AGTTCTCCCA CCCAGATGAT GAACAAGTCA
    GGCTCTGAAT GGAGTAACTC TACCCCTACT GAGGGCCCCC AGGGCACGTT TGTTCCAGAT
    ATTTTACACG GCAACTTCCA AGAGGGTGGG CACCTGGCCT CATCTTCGCC CGACTTGTGG
    ATGGGTGTGA AACAGCCCTT CAGTGTGAAG ACAGATGGTG AGAATCCTGA TATACTGACT
    CACTGTGACC ACGACAGCAA TTCCAAGGCT TCCAACAGCC CTGATGTTTG TCATGATTAT
    GAAGTAAAGC CAGAGACTAA GCAGCACATC GATGCTGGGC TGGGACCTGA AAGGGAAGCC
    AGTGAGTTAT ATCTCACTGA GCCAGAGATA GATGAAGAAT CCAGACAGGA GCCTGGGCAG
    GAATTTGTCC CTTATACTTC AGAACTGTCC TCTGAAAATG TCATTCCACT GCCTCCAGTC
    AGCGAGCAGA CAAACACAAA TGACTCATCT CAGCCTTCCT CTCACGAGTG TTTTCCTGAA
    CCTTCTGAAA TTAATGGTGA AAACAATGCA GGCTTAAAAG CATCAGAACA AGGAGCCAAC
    CCAGATATGG CACCAGTTTT GGAACCTGTT GACAGAACAA TCTCAATGAT TGAAAATGTG
    GAAACTGGTA TTTGTGTAAC TTACCAAGAG ACAACTCTGG ACGACAGTAG ATCTTCGATA
    TCAGAAGACT TTCTTCCTCA GAGGCAGACC GATGCAGGGG ACTTGTTGGA CCATGAGCAG
    CCCTCTGTTA CTGAGAATCC TGCCACGGTT CCTGATGCTT TGCTAGCCTC GGACACTTGT
    CTGGATGTCA GTGAAGCTGC CTTCGACCAC AGTCTCAGCG ATGCCTCGGG TCTCAACACA
    TCCACGGGAA CAATAGATGA CATGAGTAAG TTGACATTAT CAGAAGGCAT TCCTGAAACG
    CCATTTGATG GGGAGACAGG GAAGCATGAT ATCTGTTCCT CTGAAGCCTC GTGGGGTGAC
    TTTGAGTATG ATGTCATGGG CCAAAATATT GATGAAGACT TAATGAAAGA GCCTGAACAC
    TTTGTTTATG GTGTTGACCC TCCCTTGGAA GAAGATGCCC TGAAGCATGA GTTGGCACCT
    TACACACCTC CCTTTGATTT ATCCTATCTC ACGGAACCTA CTCCTGGCAT CAAAACCACA
    GAGGAATCTG GGACTCCAGA GGATGAGTCT CTGGGGAGCG AAGCAGCAGA GATACTGCTT
    TCTGCCCTTC CTGATGAAAG AAACCAGGAA AACCATGCTG AGACCACAGA CAGACAGCCC
    GAAGGCCAGC CAGTTGTACT GCACATTCAC GAAGACCCTG AGTCTGTTCC TTTGCCTGTC
    AGAATTGATG CCAACCCTGA GTTGTCTCCA TCACACATTG ACTGGGAAGT AGAAACAGAT
    CGTTCAGATT CACCAGCAGG TGGAGACATA GGACCACCAA GTGGTGCCAG TGAGGGAATA
    TTAGAGTTGG AAGAAGAAAA AATAATTCCT ACCAAAGAGC CTGAGCAGGT AAAATCAGAA
    CATGGGGAGG AAAGGAACAC TGAGAAGAAA GAAGATCGTC ATGCATTGCA CATGGATTAC
    ATACTTGTAA ACCATGAAGA AAATTCACTG CCAGAGCCAG AGGCCTGTGA AGCTAGAGAA
    ATCACATTGG AATTAGAAGA GCTTCACGTA GATTCCGAGG AAAAAGGGCT GCCAGAAACT
    CAGTTAGCCA GCTCCCCGGA CACAGGTCAA CCAGCTTCCT TAAATGAAAG AAATCATCTA
    TCTACAGAAA GAATGTCTTC CAAAGATGAT AAGAGATCAT CTTCTGAAAG CCCTGGGCAA
    GACCAGAGTT GGATGGTCTT GGGCCGTAGT GAGGTTAGTG ATCCATCATC AGAGACCATG
    GACTCAGGAC CTGGATGGTC TGGTGAGGCT GTGGAGCCAG CCTCTGATCA CAGCTTGGGC
    CAGAGCCCCC AAATACAGAT TCTGGAAGAA ATGAAGCCTC TAGAATCTTT AGCACTAGGG
    GAAGCCTCTG ATCTGGGCAG CCAATCACGG AGGAGCAAGA GCCGAGGCAA AGCTGGCCCT
    GATGCAGTTA TGTTGCAGCC TGTCACCCAT GACAATGAAT GGGAAATGCT TTCACCACAA
    AACATAATCC CTGAAATGGA AATGGAGGAG GAGACAGAGT TTCTTGAGCC CAGAACAAGG
    AAACCAAGAC GGAATGGACT GCTGTCAGAG GATGTGGGAA TGGACATCCA CTCTGAGGAG
    GGGATGCTGA GTCCCATTGC TGCAGACATG AGGCCAGAAC CGCCTAATTC TCTGGATCTT
    GATAGCACTC ATCCTCGGAA AATCAAACTC ACAGCCCCAA ATATCAATCT GTCTCTGGAT
    CAAAGTGAAG GGTCTGTTCT CTCTGATGAT AACTTGGACA GTCCAGATGA GATTGATATC
    AATGTGGATG AACTTGAGAC TCCCGATGAA GCAGATTCTT TTGAGTACAC TGGCCATGAG
    GATTCCACAG GCAACAAAGA TTCTGGCCAA GAGTCAGAGT CTATTCCAGA ATATACGGCA
    GAAGAGGAGC GGGAGGACAA CCGGCTGTGG AGGACCGTGG TGATCGGAGA ACAGGAGCAG
    CGGATTGACA TGAAGGTCAT CGAGCCCTAT AGGAGAGTCA TTTCTCACGG AGGAGACTCA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91264
    Clone ID Related Accession (Same CDS sequence) XM_015472513.1 , XM_015464538.1
    Accession Version XM_015464538.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGTGTGATT CTTACAGAGA TTTAACTAAT CGAAGCAAAC GTTTGGAGAA GGTCCATGTG 
    GTTATTGGAC ACAAATCCTG TGATTTGGAT TCTCTCATTT CTGCCTTCAC ATATGCTTAC
    TTTCTAGACA AGGTCAGCCC ACCAGGGGTT CTCTGTTTGC CCGTGCTGAA CATACCAAGA
    ACTGAATTCA ATTACTTCAC TGAAACAAGG TTTATTTTAG AAGAGCTAAA TATCTCTGAA
    TCATCCCACA TATTCCGAGA TGAAATTAAT CTGCATCAGC TAAATAATGA AGGGAAGTTA
    TCTCTAACAC TTGTTGGCAG CAACGTACTG GCAAGTGAGG ACAAAACTTT AGAATCAGCA
    GTTGTCAAGG TCATTAATCC GGTGGAGCAG GGCGACACCG GCTTAGAGTT CCGAGAGTCT
    TCGTCGTCTC TGGTGGTGAA AGAGATCCTC CAGGAGGCTC CCGAGCTCAT CACCGAGCAG
    CTGGCTCATC TGCTCAGAGG TAGCATCCTT TTTAAGTGGA TGGCCATGGA GCCCAAGAAG
    ATCTCAGAAA AGCAGGAGGA AATTCTTTCT ATCCTGGAAG AAAAATTTCC CAGCTTACCT
    CCAAGAGAGG ACATTATCAA CATCCTGCAG GAGACCCAGT CCAGTGCTCA GGGTTTAGGT
    ATTGAACAGA CAATGCTGAA GAATCTAAAG GAACTTTCAG ATGGAGAAAT AAAAGTGGCC
    ATTAGCACTG TGAACATGAC CCTGGAGAAC TGTACATTTC ACAGCAATAT CACCAGTGAT
    TTGAAAGCAT TCACAGAAAA GTTTGGTTTT GATGTCCTCA TCCTCCTGGC CAGTTACCTG
    TCAGAGGAGC AGCAGTCCAG ACAACAGATC GCTGTGTATT CTGAGAACCT AGAGCTGTGC
    AGTCAGATCT GCTGTGAACT GGAAGAGTGC CAGAACCCTT GCCTGGAGCT GGAACCCTTT
    GAGTGTGGCT GTGATGAGAT CCTGGTGTAC CAGCAGGAGA ACCCTTCAGT GACTTGTGAT
    CAGGTGGTTC TCCTTGTCAA GGAAGTCATC AACAGGAGGT GTCCGGAGAT GGCCTCCAAC
    AGCCGGACAT CCTCCACAGA AGCGGTGGCA GGCAGTGCCC CCCTCTCCCA GGGATCTTCA
    GGGATTATGG AGTTGTATGG TTCTGATGTA GAGCCACAGC CCAGCTCCGT GAATTTCTTG
    GAAAATCCTC CTGATCTCGG CGATTCTAAC CAGGCCCAGG TGGATGTCAA TGCGGACCTG
    GTGAGCCCAG ACAGTGGGCT GGCCACCATC AGGAGCAGCC GCTCATCCAA GGAGAGCTCC
    GTGTTCCTCA GTGACGACAG CCCTGTGGGA GAAGGGGCGG GGCCTCACCA CAGCCTCCTC
    CCAGGGTTTG ACTCCTACAG TCCCATCCCC GAAGGAGCAG TAGCGGAGGA GCATGCCCGC
    TCTGGAGAAC ATGGTCAATC ATTCGACCTT TTCAACTTTG ACCCAGCACC CAGGGTTTCT
    GGGCAGTCTC AACCATCCTC CCGTTCTGCA GACTACTCCC CAACAGATGA CTTCTTCCCC
    AACAGCGATT CCTCAGAAGG ACAGCTCACC ATGGGGTCTA AAGAACTTGA CAGGAGGGGG
    ATGGACATGC CTAATTATTC ATCCAGTTCT CTCTTGTCAG GGGCTGGCAA AGAGAGCCTC
    GTGGAATTTG ATGAGGAGTT TGTCCAGAGA CAAGAAAGTC CCGGGGATAA TTCTGAAAGA
    AATTTGAGCC TGACAGGTTT TATGCAAGAT GAATCTCCTT CTCCAGAAAG TCTAAAAACT
    GTTGGAAAGA GGATCCCACC AACACCTATG AACAGCTTTG TGGAAAGTTC ACCATCCACT
    GAAGAACCAG CTCTGCTCTG TTCAGAAGAC GTGACCCCCA AGACAGTCAA CACAGGCCAC
    CCAGGGCCAC CTCTAACCCG GGCGCGATGC AGCAGCTGGT GGGATGGTTT GGAAATCGAC
    TCTAAAAACA TCGTGGATGC ATGGAGTTCC AGCAAACAGG AATCTGTCTT CCAGAGCCCT
    GAGTCCTGGA AAGATCATAA GCCAAGCCCA GCTGATAGAA GAGCCTCAGA CTCTATATTT
    CAACCCAAGA ATCTCGACTT CCCAAAGTCA AGTCCCTGGG AGTCAGAATT TGGTCAATCT
    GGGCTGGGCA ACAAGAATAT TCAAGACTCA GATGAGAAAA ACTTCCAGTT TCAGGACATG
    CCCCCTGAGA AATCACGTTT GCTGAATGTT TCTCCTCACG GAACAAACCA CCTGATAGAA
    GACTTCGCTG CCCTGTGGCG TTCTGACCGC TCTCCCACAG CCATGCCTGA GCCGTGGGGA
    AACCCCACAG AGGATGAGGA ACCAGCTGCT GCAGCGTCAT TCCCAGCCTG GAGTGCATTT
    GGTGAAGAAG GTGATGCCAA AGCTTTGAAA AATACTTGGA ATCTGCACCC AACGAGCAAC
    GAGACACCTT CTGTGAGAGA CCCAAACGAG TGGGCCATGG CCAAAAGTGG GATTGCTTTC
    CCTTCAGAAG ACCTCGTGGA CAGTTCACCC AGTGAGGCAA ATAATGAAGC GGTTCAAAAA
    ATCTGGGGCA AGAAGAACCA TGACCCCAGG GATGATATTC TCATATCTGG AAATCCCACA
    TCTGATCTGG ATCATCCATG GAATAGTTCT AAGCCAGCAA AGGACCATCA GAACAGTGTG
    GTGGACCCAA CAATTAGGGG CAAAATGTAC AAAAATGTAG ATTCTTGGAA TCTTTTCGAG
    GAGAGTAACA AGGAAGGAGG TTCAGATGTC CCAGCACCTT GGGAGGACTC CTTCTTATCC
    TATAAATGTT CTGACTACAG TGCATCCAAC GTCAGGGAAG ACTCAATGCC CTCCCCCTTA
    GATACCAACT ACTCCACCTC TGACTCTTAT ACTTCACCAA CATTGGCCAA AGAGGAGAAA
    GAAACCAAAG ACAAGCCACT CACTAAAGAG GAGGGTTTTG AGTCACAAGA TGCTAATTGT
    ACTGCAGGGG GCGCTGACAT GCCTCCTCAG TCCCCGCAGC AGCCACCAAG AAATCGAATG
    AGTTCAGGTC CTGGGAACCT GGAAATGTGG GCTTCATCTC AGGCAGATGG TGGCACAGAA
    ATAAATGCCA CCCATAGCCC AGATAAAGAT CTACTCCAAG TAGAGCCCAC GGATGATCAG
    AATGTCTCCT TTGAGGATGA CATGGGGGAA AGCAGCCAGT CCAGTTATGA CGACCCCAGC
    ATGATGCAGC TGTACAACGA GACGAACCGA CAGCTCACAC TTCTGCACAG CAGTGTTCAC
    GCCCGGCAGG CAGCCCCTGA CAGCCTCGAC ACGTGGAACA GAGTGATTCT GGAGGACACT
    CAGTCTACTG CAACCATCTC GGATATGGAC AATGACTTGG ACTGGGATGA CTGCAGTGCG
    GGCGTGGCCA TCACTGGTGA AGGTCAGGCA CAAGGGTACA TGGCGGAAGG TAGCGAACCT
    GAAACCCGAT TTTCAGTGAG ACAGGTAGAA CCCTGGGGCG TGGAGTATCA GGAGGCAAAT
    CAGTCAGACT GGGAACTCCC TGCCTCTTCT GAGCACAGCC TGGACGTGGC TCCTGATGAA
    TACCACGTGC TGAATGAGAA AAGTGGGCAG CTGATCGCGA ATAGTATTTG GGATTCTGTC
    ATGAGAGATA ATGCCATGTC ATCATTAATA TTACCTAGCC CATCATATGC TACAGATACA
    GAACAGAGCA GATCACCTCC TGAAACAACC AGCTCATTAG AAAAAATTCA GGATAGTCAC
    ATTGCAGACG TGCTGGAAGC CTCTGAAGCC AGTGAGTCTG AAACAATCGA TCCTGACAAG
    CAGGACACAT GCAGAGGAAC CCTGCCAAGT GATTCGGCAT ATTTGGTGAC CAGACTTGAG
    AATCCAGAGC ATTTTGCATG CTCAGATCCC TGGAAGGGTC CGAGCTATGG ACAAAAGGAA
    AGTGAGAAGG AATTCCACGG AGCTGCTGAT AGGGAGAATT CCAGTGATGA GCTAATGGAT
    GGAACCTCAG AACCTTCTGG AAAAGGGCCA GGTAGCCAGG AATGCCCATC CCCAGCTGCA
    TCTGATCATC AAGAAATCTC CAATGAACCA GGCAAAATCA GAAATTTTTC AGTGACTTCC
    AGTCCTCAGA CCAAGGAACC CCAGGAAGTA TTAGAGCAGG AGAAGGGGTC TTACACTCCA
    GACCCCTGTG ATGTGCAAAC AGAGGTGTCC GAAGATCACT TGCCAGCAAG AGAGACCTGT
    GAAGAGCTCC TCATAAGCCC CAGAAGAAAT TCAAGTGAAA CTACTGAAAT TCCAGGTAGG
    CTGAATTCCA CAAAAAGTGT GTGTCTACCT CAAGTGGATG TGGAGAAAGC TGAAGAATGT
    GACATTCTGG AGCCAGAGAA AATTGACCAA GAGCCACCTG ATGAATGTCC CCAAGGCCTT
    GACTCTTGGG ATGGCCCTAA CGACAGTGAT GTGCTGGTCA GTGGTGCAAG TTCTGAGATA
    ACTATGAATC TCGAAGTTAG AAATACTGAA AACAGCCCTC CAAGAGCCAA TTTATCAGGA
    AATGATGACA GAGATAGTAT TTCTAGTGAG TATACACTTT CAAGTGCATC TAGTCCTGAC
    ATAAATGATT CTTCAGCTAC ATTGCCTTCC TGGGACCAAG GACTCAATAC TGGGCATCAG
    AAGGAAAATC AAAATGATTG GAGTACACAG AATCGCCAAG AATCTGAACT AATGACTACT
    GATGGTCATA CAGAAGTAGT TGCTGAAATG CAGGGTTTGG AGAAAAACAG AATGGACAGG
    TTTGAAAGGA GCTTAGATCA CAAGGTTCCT AAATTTTTAG AGTTTTGGGG TGACTCAATA
    GATGGTGATT CCTTTTCTTC TTTATCCAGC CCAGAAACAG GTAGATATTC TGAATATTCA
    GATGCGTATC AGGAAAGAAA GCTAGTGGTC AGCCATCAGG AGAAAAATGA ACGTGATCTT
    CCTGAAACTG TACAGCCAGA GGACACCAGC TTCATTTCAA CAAGCTCAGG TTCTGATGAT
    GGTGAAGGCT ATGAAGAGCC AGTGGAGAAA GAGATCCATT CGGATACTTG TCAAGTGGCT
    CCGAGTGAAC TCAGAGCTTG GGATTCATTG AATAAATCTA ATAAGTCCTT GGCCACAGCA
    GATCCTGAAT CTGAGCAATG TTCTGTCTAT AGAGGCAGTC CAGAGCAAGC AGAGAATGAG
    AATAAGTCAA ACCCATCCTG TGATGATCAA CAAAGCAGCC CTGATCACTG GAATTTCTCA
    CCAGTAAGGA AGACTGAATT ACAGGTAACA TCAGTCGATA AGGAGACAAG GTCTTCTCCA
    GAAATAGGGA GGACAGGAGA TATCATATGG CAAATATCTC CCAAAGCTGA ACCAAATAAT
    GAAAATTACT CTAAATTGGA AATGGCTGAC TTCTCAACTG AGAGCACGGA GTGGTGGAAG
    CCCTCCCTAC AGGAAGGACG ACCAATCGAG AGTGCATTTG AAGGAGAATT ATCTAACTCA
    AGTGCTGTGC TAGAAATGAA TTCCTCAGTG TGCGAAAACA TGGGTCCTTG GGGAGTGCCC
    ATTCAGGGTG ATACTGAATC CCTGGCACTA CATGGTACCA ATCCTTTCAA TGATAACCAT
    CAGTCACCCT TTCTAGATCA TAATGGGGAG AATGCCCACG AGCAGCTTTG GAACATTCAA
    CCAAGGCAGC CAGACCCAGA AGCTGATCAG CTTAACCAAC TTGTAATATT GGAACAAATT
    AAAGACACAG ATTCCAGAGA ACAAACCTTC ATGTCTTCTG CCGGTGACGA ACTCTCCTCT
    GAAGTGCCTA CGCAAGAACA GTGTCAGAAT TCAGTGCTGT CTGCCTGGGA CCAGCCAGAC
    TCGTCTTGTA CTTACGCAGA TGAAAATGGA TGCATTGTAT CTGGTGTAGC AACAACTGAG
    TGTCAAGAAA CAAATTGGTG GGAAGAAGAA AAATCACACC CAAGTGAAAT GACAAATTCA
    AACATGACCT CTGAAGATTT CCCTGCTGTC AGTTCTCCCA CCCAGATGAT GAACAAGTCA
    GGCTCTGAAT GGAGTAACTC TACCCCTACT GAGGGCCCCC AGGGCACGTT TGTTCCAGAT
    ATTTTACACG GCAACTTCCA AGAGGGTGGG CACCTGGCCT CATCTTCGCC CGACTTGTGG
    ATGGGTGTGA AACAGCCCTT CAGTGTGAAG ACAGATGGTG AGAATCCTGA TATACTGACT
    CACTGTGACC ACGACAGCAA TTCCAAGGCT TCCAACAGCC CTGATGTTTG TCATGATTAT
    GAAGTAAAGC CAGAGACTAA GCAGCACATC GATGCTGGGC TGGGACCTGA AAGGGAAGCC
    AGTGAGTTAT ATCTCACTGA GCCAGAGATA GATGAAGAAT CCAGACAGGA GCCTGGGCAG
    GAATTTGTCC CTTATACTTC AGAACTGTCC TCTGAAAATG TCATTCCACT GCCTCCAGTC
    AGCGAGCAGA CAAACACAAA TGACTCATCT CAGCCTTCCT CTCACGAGTG TTTTCCTGAA
    CCTTCTGAAA TTAATGGTGA AAACAATGCA GGCTTAAAAG CATCAGAACA AGGAGCCAAC
    CCAGATATGG CACCAGTTTT GGAACCTGTT GACAGAACAA TCTCAATGAT TGAAAATGTG
    GAAACTGGTA TTTGTGTAAC TTACCAAGAG ACAACTCTGG ACGACAGTAG ATCTTCGATA
    TCAGAAGACT TTCTTCCTCA GAGGCAGACC GATGCAGGGG ACTTGTTGGA CCATGAGCAG
    CCCTCTGTTA CTGAGAATCC TGCCACGGTT CCTGATGCTT TGCTAGCCTC GGACACTTGT
    CTGGATGTCA GTGAAGCTGC CTTCGACCAC AGTCTCAGCG ATGCCTCGGG TCTCAACACA
    TCCACGGGAA CAATAGATGA CATGAGTAAG TTGACATTAT CAGAAGGCAT TCCTGAAACG
    CCATTTGATG GGGAGACAGG GAAGCATGAT ATCTGTTCCT CTGAAGCCTC GTGGGGTGAC
    TTTGAGTATG ATGTCATGGG CCAAAATATT GATGAAGACT TAATGAAAGA GCCTGAACAC
    TTTGTTTATG GTGTTGACCC TCCCTTGGAA GAAGATGCCC TGAAGCATGA GTTGGCACCT
    TACACACCTC CCTTTGATTT ATCCTATCTC ACGGAACCTA CTCCTGGCAT CAAAACCACA
    GAGGAATCTG GGACTCCAGA GGATGAGTCT CTGGGGAGCG AAGCAGCAGA GATACTGCTT
    TCTGCCCTTC CTGATGAAAG AAACCAGGAA AACCATGCTG AGACCACAGA CAGACAGCCC
    GAAGGCCAGC CAGTTGTACT GCACATTCAC GAAGACCCTG AGTCTGTTCC TTTGCCTGTC
    AGAATTGATG CCAACCCTGA GTTGTCTCCA TCACACATTG ACTGGGAAGT AGAAACAGAT
    CGTTCAGATT CACCAGCAGG TGGAGACATA GGACCACCAA GTGGTGCCAG TGAGGGAATA
    TTAGAGTTGG AAGAAGAAAA AATAATTCCT ACCAAAGAGC CTGAGCAGGT AAAATCAGAA
    CATGGGGAGG AAAGGAACAC TGAGAAGAAA GAAGATCGTC ATGCATTGCA CATGGATTAC
    ATACTTGTAA ACCATGAAGA AAATTCACTG CCAGAGCCAG AGGCCTGTGA AGCTAGAGAA
    ATCACATTGG AATTAGAAGA GCTTCACGTA GATTCCGAGG AAAAAGGGCT GCCAGAAACT
    CAGTTAGCCA GCTCCCCGGA CACAGGTCAA CCAGCTTCCT TAAATGAAAG AAATCATCTA
    TCTACAGAAA GAATGTCTTC CAAAGATGAT AAGAGATCAT CTTCTGAAAG CCCTGGGCAA
    GACCAGAGTT GGATGGTCTT GGGCCGTAGT GAGGTTAGTG ATCCATCATC AGAGACCATG
    GACTCAGGAC CTGGATGGTC TGGTGAGGCT GTGGAGCCAG CCTCTGATCA CAGCTTGGGC
    CAGAGCCCCC AAATACAGAT TCTGGAAGAA ATGAAGCCTC TAGAATCTTT AGCACTAGGG
    GAAGCCTCTG ATCTGGGCAG CCAATCACGG AGGAGCAAGA GCCGAGGCAA AGCTGGCCCT
    GATGCAGTTA TGTTGCAGCC TGTCACCCAT GACAATGAAT GGGAAATGCT TTCACCACAA
    AACATAATCC CTGAAATGGA AATGGAGGAG GAGACAGAGT TTCTTGAGCC CAGAACAAGG
    AAACCAAGAC GGAATGGACT GCTGTCAGAG GATGTGGGAA TGGACATCCA CTCTGAGGAG
    GGGATGCTGA GTCCCATTGC TGCAGACATG AGGCCAGAAC CGCCTAATTC TCTGGATCTT
    GATAGCACTC ATCCTCGGAA AATCAAACTC ACAGCCCCAA ATATCAATCT GTCTCTGGAT
    CAAAGTGAAG GGTCTGTTCT CTCTGATGAT AACTTGGACA GTCCAGATGA GATTGATATC
    AATGTGGATG AACTTGAGAC TCCCGATGAA GCAGATTCTT TTGAGTACAC TGGCCATGAG
    GATTCCACAG GCAACAAAGA TTCTGGCCAA GAGTCAGAGT CTATTCCAGA ATATACGGCA
    GAAGAGGAGC GGGAGGACAA CCGGCTGTGG AGGACCGTGG TGATCGGAGA ACAGGAGCAG
    CGGATTGACA TGAAGGTCAT CGAGCCCTAT AGGAGAGTCA TTTCTCACGG AGGAGACTCA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320024.1
    CDS41..9280
    Translation

    Target ORF information:

    RefSeq Version XM_015464538.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464538.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    ATGTGTGATT CTTACAGAGA TTTAACTAAT CGAAGCAAAC GTTTGGAGAA GGTCCATGTG 
    GTTATTGGAC ACAAATCCTG TGATTTGGAT TCTCTCATTT CTGCCTTCAC ATATGCTTAC
    TTTCTAGACA AGGTCAGCCC ACCAGGGGTT CTCTGTTTGC CCGTGCTGAA CATACCAAGA
    ACTGAATTCA ATTACTTCAC TGAAACAAGG TTTATTTTAG AAGAGCTAAA TATCTCTGAA
    TCATCCCACA TATTCCGAGA TGAAATTAAT CTGCATCAGC TAAATAATGA AGGGAAGTTA
    TCTCTAACAC TTGTTGGCAG CAACGTACTG GCAAGTGAGG ACAAAACTTT AGAATCAGCA
    GTTGTCAAGG TCATTAATCC GGTGGAGCAG GGCGACACCG GCTTAGAGTT CCGAGAGTCT
    TCGTCGTCTC TGGTGGTGAA AGAGATCCTC CAGGAGGCTC CCGAGCTCAT CACCGAGCAG
    CTGGCTCATC TGCTCAGAGG TAGCATCCTT TTTAAGTGGA TGGCCATGGA GCCCAAGAAG
    ATCTCAGAAA AGCAGGAGGA AATTCTTTCT ATCCTGGAAG AAAAATTTCC CAGCTTACCT
    CCAAGAGAGG ACATTATCAA CATCCTGCAG GAGACCCAGT CCAGTGCTCA GGGTTTAGGT
    ATTGAACAGA CAATGCTGAA GAATCTAAAG GAACTTTCAG ATGGAGAAAT AAAAGTGGCC
    ATTAGCACTG TGAACATGAC CCTGGAGAAC TGTACATTTC ACAGCAATAT CACCAGTGAT
    TTGAAAGCAT TCACAGAAAA GTTTGGTTTT GATGTCCTCA TCCTCCTGGC CAGTTACCTG
    TCAGAGGAGC AGCAGTCCAG ACAACAGATC GCTGTGTATT CTGAGAACCT AGAGCTGTGC
    AGTCAGATCT GCTGTGAACT GGAAGAGTGC CAGAACCCTT GCCTGGAGCT GGAACCCTTT
    GAGTGTGGCT GTGATGAGAT CCTGGTGTAC CAGCAGGAGA ACCCTTCAGT GACTTGTGAT
    CAGGTGGTTC TCCTTGTCAA GGAAGTCATC AACAGGAGGT GTCCGGAGAT GGCCTCCAAC
    AGCCGGACAT CCTCCACAGA AGCGGTGGCA GGCAGTGCCC CCCTCTCCCA GGGATCTTCA
    GGGATTATGG AGTTGTATGG TTCTGATGTA GAGCCACAGC CCAGCTCCGT GAATTTCTTG
    GAAAATCCTC CTGATCTCGG CGATTCTAAC CAGGCCCAGG TGGATGTCAA TGCGGACCTG
    GTGAGCCCAG ACAGTGGGCT GGCCACCATC AGGAGCAGCC GCTCATCCAA GGAGAGCTCC
    GTGTTCCTCA GTGACGACAG CCCTGTGGGA GAAGGGGCGG GGCCTCACCA CAGCCTCCTC
    CCAGGGTTTG ACTCCTACAG TCCCATCCCC GAAGGAGCAG TAGCGGAGGA GCATGCCCGC
    TCTGGAGAAC ATGGTCAATC ATTCGACCTT TTCAACTTTG ACCCAGCACC CAGGGTTTCT
    GGGCAGTCTC AACCATCCTC CCGTTCTGCA GACTACTCCC CAACAGATGA CTTCTTCCCC
    AACAGCGATT CCTCAGAAGG ACAGCTCACC ATGGGGTCTA AAGAACTTGA CAGGAGGGGG
    ATGGACATGC CTAATTATTC ATCCAGTTCT CTCTTGTCAG GGGCTGGCAA AGAGAGCCTC
    GTGGAATTTG ATGAGGAGTT TGTCCAGAGA CAAGAAAGTC CCGGGGATAA TTCTGAAAGA
    AATTTGAGCC TGACAGGTTT TATGCAAGAT GAATCTCCTT CTCCAGAAAG TCTAAAAACT
    GTTGGAAAGA GGATCCCACC AACACCTATG AACAGCTTTG TGGAAAGTTC ACCATCCACT
    GAAGAACCAG CTCTGCTCTG TTCAGAAGAC GTGACCCCCA AGACAGTCAA CACAGGCCAC
    CCAGGGCCAC CTCTAACCCG GGCGCGATGC AGCAGCTGGT GGGATGGTTT GGAAATCGAC
    TCTAAAAACA TCGTGGATGC ATGGAGTTCC AGCAAACAGG AATCTGTCTT CCAGAGCCCT
    GAGTCCTGGA AAGATCATAA GCCAAGCCCA GCTGATAGAA GAGCCTCAGA CTCTATATTT
    CAACCCAAGA ATCTCGACTT CCCAAAGTCA AGTCCCTGGG AGTCAGAATT TGGTCAATCT
    GGGCTGGGCA ACAAGAATAT TCAAGACTCA GATGAGAAAA ACTTCCAGTT TCAGGACATG
    CCCCCTGAGA AATCACGTTT GCTGAATGTT TCTCCTCACG GAACAAACCA CCTGATAGAA
    GACTTCGCTG CCCTGTGGCG TTCTGACCGC TCTCCCACAG CCATGCCTGA GCCGTGGGGA
    AACCCCACAG AGGATGAGGA ACCAGCTGCT GCAGCGTCAT TCCCAGCCTG GAGTGCATTT
    GGTGAAGAAG GTGATGCCAA AGCTTTGAAA AATACTTGGA ATCTGCACCC AACGAGCAAC
    GAGACACCTT CTGTGAGAGA CCCAAACGAG TGGGCCATGG CCAAAAGTGG GATTGCTTTC
    CCTTCAGAAG ACCTCGTGGA CAGTTCACCC AGTGAGGCAA ATAATGAAGC GGTTCAAAAA
    ATCTGGGGCA AGAAGAACCA TGACCCCAGG GATGATATTC TCATATCTGG AAATCCCACA
    TCTGATCTGG ATCATCCATG GAATAGTTCT AAGCCAGCAA AGGACCATCA GAACAGTGTG
    GTGGACCCAA CAATTAGGGG CAAAATGTAC AAAAATGTAG ATTCTTGGAA TCTTTTCGAG
    GAGAGTAACA AGGAAGGAGG TTCAGATGTC CCAGCACCTT GGGAGGACTC CTTCTTATCC
    TATAAATGTT CTGACTACAG TGCATCCAAC GTCAGGGAAG ACTCAATGCC CTCCCCCTTA
    GATACCAACT ACTCCACCTC TGACTCTTAT ACTTCACCAA CATTGGCCAA AGAGGAGAAA
    GAAACCAAAG ACAAGCCACT CACTAAAGAG GAGGGTTTTG AGTCACAAGA TGCTAATTGT
    ACTGCAGGGG GCGCTGACAT GCCTCCTCAG TCCCCGCAGC AGCCACCAAG AAATCGAATG
    AGTTCAGGTC CTGGGAACCT GGAAATGTGG GCTTCATCTC AGGCAGATGG TGGCACAGAA
    ATAAATGCCA CCCATAGCCC AGATAAAGAT CTACTCCAAG TAGAGCCCAC GGATGATCAG
    AATGTCTCCT TTGAGGATGA CATGGGGGAA AGCAGCCAGT CCAGTTATGA CGACCCCAGC
    ATGATGCAGC TGTACAACGA GACGAACCGA CAGCTCACAC TTCTGCACAG CAGTGTTCAC
    GCCCGGCAGG CAGCCCCTGA CAGCCTCGAC ACGTGGAACA GAGTGATTCT GGAGGACACT
    CAGTCTACTG CAACCATCTC GGATATGGAC AATGACTTGG ACTGGGATGA CTGCAGTGCG
    GGCGTGGCCA TCACTGGTGA AGGTCAGGCA CAAGGGTACA TGGCGGAAGG TAGCGAACCT
    GAAACCCGAT TTTCAGTGAG ACAGGTAGAA CCCTGGGGCG TGGAGTATCA GGAGGCAAAT
    CAGTCAGACT GGGAACTCCC TGCCTCTTCT GAGCACAGCC TGGACGTGGC TCCTGATGAA
    TACCACGTGC TGAATGAGAA AAGTGGGCAG CTGATCGCGA ATAGTATTTG GGATTCTGTC
    ATGAGAGATA ATGCCATGTC ATCATTAATA TTACCTAGCC CATCATATGC TACAGATACA
    GAACAGAGCA GATCACCTCC TGAAACAACC AGCTCATTAG AAAAAATTCA GGATAGTCAC
    ATTGCAGACG TGCTGGAAGC CTCTGAAGCC AGTGAGTCTG AAACAATCGA TCCTGACAAG
    CAGGACACAT GCAGAGGAAC CCTGCCAAGT GATTCGGCAT ATTTGGTGAC CAGACTTGAG
    AATCCAGAGC ATTTTGCATG CTCAGATCCC TGGAAGGGTC CGAGCTATGG ACAAAAGGAA
    AGTGAGAAGG AATTCCACGG AGCTGCTGAT AGGGAGAATT CCAGTGATGA GCTAATGGAT
    GGAACCTCAG AACCTTCTGG AAAAGGGCCA GGTAGCCAGG AATGCCCATC CCCAGCTGCA
    TCTGATCATC AAGAAATCTC CAATGAACCA GGCAAAATCA GAAATTTTTC AGTGACTTCC
    AGTCCTCAGA CCAAGGAACC CCAGGAAGTA TTAGAGCAGG AGAAGGGGTC TTACACTCCA
    GACCCCTGTG ATGTGCAAAC AGAGGTGTCC GAAGATCACT TGCCAGCAAG AGAGACCTGT
    GAAGAGCTCC TCATAAGCCC CAGAAGAAAT TCAAGTGAAA CTACTGAAAT TCCAGGTAGG
    CTGAATTCCA CAAAAAGTGT GTGTCTACCT CAAGTGGATG TGGAGAAAGC TGAAGAATGT
    GACATTCTGG AGCCAGAGAA AATTGACCAA GAGCCACCTG ATGAATGTCC CCAAGGCCTT
    GACTCTTGGG ATGGCCCTAA CGACAGTGAT GTGCTGGTCA GTGGTGCAAG TTCTGAGATA
    ACTATGAATC TCGAAGTTAG AAATACTGAA AACAGCCCTC CAAGAGCCAA TTTATCAGGA
    AATGATGACA GAGATAGTAT TTCTAGTGAG TATACACTTT CAAGTGCATC TAGTCCTGAC
    ATAAATGATT CTTCAGCTAC ATTGCCTTCC TGGGACCAAG GACTCAATAC TGGGCATCAG
    AAGGAAAATC AAAATGATTG GAGTACACAG AATCGCCAAG AATCTGAACT AATGACTACT
    GATGGTCATA CAGAAGTAGT TGCTGAAATG CAGGGTTTGG AGAAAAACAG AATGGACAGG
    TTTGAAAGGA GCTTAGATCA CAAGGTTCCT AAATTTTTAG AGTTTTGGGG TGACTCAATA
    GATGGTGATT CCTTTTCTTC TTTATCCAGC CCAGAAACAG GTAGATATTC TGAATATTCA
    GATGCGTATC AGGAAAGAAA GCTAGTGGTC AGCCATCAGG AGAAAAATGA ACGTGATCTT
    CCTGAAACTG TACAGCCAGA GGACACCAGC TTCATTTCAA CAAGCTCAGG TTCTGATGAT
    GGTGAAGGCT ATGAAGAGCC AGTGGAGAAA GAGATCCATT CGGATACTTG TCAAGTGGCT
    CCGAGTGAAC TCAGAGCTTG GGATTCATTG AATAAATCTA ATAAGTCCTT GGCCACAGCA
    GATCCTGAAT CTGAGCAATG TTCTGTCTAT AGAGGCAGTC CAGAGCAAGC AGAGAATGAG
    AATAAGTCAA ACCCATCCTG TGATGATCAA CAAAGCAGCC CTGATCACTG GAATTTCTCA
    CCAGTAAGGA AGACTGAATT ACAGGTAACA TCAGTCGATA AGGAGACAAG GTCTTCTCCA
    GAAATAGGGA GGACAGGAGA TATCATATGG CAAATATCTC CCAAAGCTGA ACCAAATAAT
    GAAAATTACT CTAAATTGGA AATGGCTGAC TTCTCAACTG AGAGCACGGA GTGGTGGAAG
    CCCTCCCTAC AGGAAGGACG ACCAATCGAG AGTGCATTTG AAGGAGAATT ATCTAACTCA
    AGTGCTGTGC TAGAAATGAA TTCCTCAGTG TGCGAAAACA TGGGTCCTTG GGGAGTGCCC
    ATTCAGGGTG ATACTGAATC CCTGGCACTA CATGGTACCA ATCCTTTCAA TGATAACCAT
    CAGTCACCCT TTCTAGATCA TAATGGGGAG AATGCCCACG AGCAGCTTTG GAACATTCAA
    CCAAGGCAGC CAGACCCAGA AGCTGATCAG CTTAACCAAC TTGTAATATT GGAACAAATT
    AAAGACACAG ATTCCAGAGA ACAAACCTTC ATGTCTTCTG CCGGTGACGA ACTCTCCTCT
    GAAGTGCCTA CGCAAGAACA GTGTCAGAAT TCAGTGCTGT CTGCCTGGGA CCAGCCAGAC
    TCGTCTTGTA CTTACGCAGA TGAAAATGGA TGCATTGTAT CTGGTGTAGC AACAACTGAG
    TGTCAAGAAA CAAATTGGTG GGAAGAAGAA AAATCACACC CAAGTGAAAT GACAAATTCA
    AACATGACCT CTGAAGATTT CCCTGCTGTC AGTTCTCCCA CCCAGATGAT GAACAAGTCA
    GGCTCTGAAT GGAGTAACTC TACCCCTACT GAGGGCCCCC AGGGCACGTT TGTTCCAGAT
    ATTTTACACG GCAACTTCCA AGAGGGTGGG CACCTGGCCT CATCTTCGCC CGACTTGTGG
    ATGGGTGTGA AACAGCCCTT CAGTGTGAAG ACAGATGGTG AGAATCCTGA TATACTGACT
    CACTGTGACC ACGACAGCAA TTCCAAGGCT TCCAACAGCC CTGATGTTTG TCATGATTAT
    GAAGTAAAGC CAGAGACTAA GCAGCACATC GATGCTGGGC TGGGACCTGA AAGGGAAGCC
    AGTGAGTTAT ATCTCACTGA GCCAGAGATA GATGAAGAAT CCAGACAGGA GCCTGGGCAG
    GAATTTGTCC CTTATACTTC AGAACTGTCC TCTGAAAATG TCATTCCACT GCCTCCAGTC
    AGCGAGCAGA CAAACACAAA TGACTCATCT CAGCCTTCCT CTCACGAGTG TTTTCCTGAA
    CCTTCTGAAA TTAATGGTGA AAACAATGCA GGCTTAAAAG CATCAGAACA AGGAGCCAAC
    CCAGATATGG CACCAGTTTT GGAACCTGTT GACAGAACAA TCTCAATGAT TGAAAATGTG
    GAAACTGGTA TTTGTGTAAC TTACCAAGAG ACAACTCTGG ACGACAGTAG ATCTTCGATA
    TCAGAAGACT TTCTTCCTCA GAGGCAGACC GATGCAGGGG ACTTGTTGGA CCATGAGCAG
    CCCTCTGTTA CTGAGAATCC TGCCACGGTT CCTGATGCTT TGCTAGCCTC GGACACTTGT
    CTGGATGTCA GTGAAGCTGC CTTCGACCAC AGTCTCAGCG ATGCCTCGGG TCTCAACACA
    TCCACGGGAA CAATAGATGA CATGAGTAAG TTGACATTAT CAGAAGGCAT TCCTGAAACG
    CCATTTGATG GGGAGACAGG GAAGCATGAT ATCTGTTCCT CTGAAGCCTC GTGGGGTGAC
    TTTGAGTATG ATGTCATGGG CCAAAATATT GATGAAGACT TAATGAAAGA GCCTGAACAC
    TTTGTTTATG GTGTTGACCC TCCCTTGGAA GAAGATGCCC TGAAGCATGA GTTGGCACCT
    TACACACCTC CCTTTGATTT ATCCTATCTC ACGGAACCTA CTCCTGGCAT CAAAACCACA
    GAGGAATCTG GGACTCCAGA GGATGAGTCT CTGGGGAGCG AAGCAGCAGA GATACTGCTT
    TCTGCCCTTC CTGATGAAAG AAACCAGGAA AACCATGCTG AGACCACAGA CAGACAGCCC
    GAAGGCCAGC CAGTTGTACT GCACATTCAC GAAGACCCTG AGTCTGTTCC TTTGCCTGTC
    AGAATTGATG CCAACCCTGA GTTGTCTCCA TCACACATTG ACTGGGAAGT AGAAACAGAT
    CGTTCAGATT CACCAGCAGG TGGAGACATA GGACCACCAA GTGGTGCCAG TGAGGGAATA
    TTAGAGTTGG AAGAAGAAAA AATAATTCCT ACCAAAGAGC CTGAGCAGGT AAAATCAGAA
    CATGGGGAGG AAAGGAACAC TGAGAAGAAA GAAGATCGTC ATGCATTGCA CATGGATTAC
    ATACTTGTAA ACCATGAAGA AAATTCACTG CCAGAGCCAG AGGCCTGTGA AGCTAGAGAA
    ATCACATTGG AATTAGAAGA GCTTCACGTA GATTCCGAGG AAAAAGGGCT GCCAGAAACT
    CAGTTAGCCA GCTCCCCGGA CACAGGTCAA CCAGCTTCCT TAAATGAAAG AAATCATCTA
    TCTACAGAAA GAATGTCTTC CAAAGATGAT AAGAGATCAT CTTCTGAAAG CCCTGGGCAA
    GACCAGAGTT GGATGGTCTT GGGCCGTAGT GAGGTTAGTG ATCCATCATC AGAGACCATG
    GACTCAGGAC CTGGATGGTC TGGTGAGGCT GTGGAGCCAG CCTCTGATCA CAGCTTGGGC
    CAGAGCCCCC AAATACAGAT TCTGGAAGAA ATGAAGCCTC TAGAATCTTT AGCACTAGGG
    GAAGCCTCTG ATCTGGGCAG CCAATCACGG AGGAGCAAGA GCCGAGGCAA AGCTGGCCCT
    GATGCAGTTA TGTTGCAGCC TGTCACCCAT GACAATGAAT GGGAAATGCT TTCACCACAA
    AACATAATCC CTGAAATGGA AATGGAGGAG GAGACAGAGT TTCTTGAGCC CAGAACAAGG
    AAACCAAGAC GGAATGGACT GCTGTCAGAG GATGTGGGAA TGGACATCCA CTCTGAGGAG
    GGGATGCTGA GTCCCATTGC TGCAGACATG AGGCCAGAAC CGCCTAATTC TCTGGATCTT
    GATAGCACTC ATCCTCGGAA AATCAAACTC ACAGCCCCAA ATATCAATCT GTCTCTGGAT
    CAAAGTGAAG GGTCTGTTCT CTCTGATGAT AACTTGGACA GTCCAGATGA GATTGATATC
    AATGTGGATG AACTTGAGAC TCCCGATGAA GCAGATTCTT TTGAGTACAC TGGCCATGAG
    GATTCCACAG GCAACAAAGA TTCTGGCCAA GAGTCAGAGT CTATTCCAGA ATATACGGCA
    GAAGAGGAGC GGGAGGACAA CCGGCTGTGG AGGACCGTGG TGATCGGAGA ACAGGAGCAG
    CGGATTGACA TGAAGGTCAT CGAGCCCTAT AGGAGAGTCA TTTCTCACGG AGGAGACTCA
    GGATACTATG GGGATGGCCT AAATGCCATC ATTGTGTTTG CCGCCTGTTT TCTGCCAGAC
    AGCAGTCGGG CGGACTACCA CTATGTCATG GAAAATCTTT TCCTATATGT AATAAGTACT
    TTAGAGTTGA TGGTAGCTGA AGACTATATG ATTGTGTACT TGAATGGTGC AACCCCAAGA
    CGGAAGATGC CAGGGTTAGG CTGGATGAAG AAATGTTACC AGATGATTGA CAGACGGTTG
    AGGAAGAATT TAAAATCATT CATCATTGTT CATCCATCTT GGTTCATCCG AACAATCCTT
    GCAGTTACAC GACCTTTTAT AAGTTCCAAA TTCAGCAGTA AAATTAAATA CGTCAGTAGT
    TTAGCAGAAC TCAGTGGACT GATCCCAATG GATTGCATCC ATATTCCAGA GAGCATCATC
    AAACTGGATG AAGATCTGAG GGAAGCATCT GAAGCAGCTA AAACTAGCTG CCTTTACAAT
    GATCCAGAAA TGTCTTCTAT GGAGAAGGAT ATTAATCTGA AGCTGAAAGA AAAGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91265
    Clone ID Related Accession (Same CDS sequence) XM_015464539.1 , XM_015472514.1
    Accession Version XM_015464539.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645277.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAGGA TTCCACAGGC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCAGA AGAGGAGCGG
    GAGGACAACC GGCTGTGGAG GACCGTGGTG ATCGGAGAAC AGGAGCAGCG GATTGACATG
    AAGGTCATCG AGCCCTATAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GATGGCCTAA ATGCCATCAT TGTGTTTGCC GCCTGTTTTC TGCCAGACAG CAGTCGGGCG
    GACTACCACT ATGTCATGGA AAATCTTTTC CTATATGTAA TAAGTACTTT AGAGTTGATG
    GTAGCTGAAG ACTATATGAT TGTGTACTTG AATGGTGCAA CCCCAAGACG GAAGATGCCA
    GGGTTAGGCT GGATGAAGAA ATGTTACCAG ATGATTGACA GACGGTTGAG GAAGAATTTA
    AAATCATTCA TCATTGTTCA TCCATCTTGG TTCATCCGAA CAATCCTTGC AGTTACACGA
    CCTTTTATAA GTTCCAAATT CAGCAGTAAA ATTAAATACG TCAGTAGTTT AGCAGAACTC
    AGTGGACTGA TCCCAATGGA TTGCATCCAT ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GATCTGAGGG AAGCATCTGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TAATCTGAAG CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320025.1
    CDS190..9237
    Translation

    Target ORF information:

    RefSeq Version XM_015464539.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464539.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAGGA TTCCACAGGC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCAGA AGAGGAGCGG
    GAGGACAACC GGCTGTGGAG GACCGTGGTG ATCGGAGAAC AGGAGCAGCG GATTGACATG
    AAGGTCATCG AGCCCTATAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GATGGCCTAA ATGCCATCAT TGTGTTTGCC GCCTGTTTTC TGCCAGACAG CAGTCGGGCG
    GACTACCACT ATGTCATGGA AAATCTTTTC CTATATGTAA TAAGTACTTT AGAGTTGATG
    GTAGCTGAAG ACTATATGAT TGTGTACTTG AATGGTGCAA CCCCAAGACG GAAGATGCCA
    GGGTTAGGCT GGATGAAGAA ATGTTACCAG ATGATTGACA GACGGTTGAG GAAGAATTTA
    AAATCATTCA TCATTGTTCA TCCATCTTGG TTCATCCGAA CAATCCTTGC AGTTACACGA
    CCTTTTATAA GTTCCAAATT CAGCAGTAAA ATTAAATACG TCAGTAGTTT AGCAGAACTC
    AGTGGACTGA TCCCAATGGA TTGCATCCAT ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GATCTGAGGG AAGCATCTGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TAATCTGAAG CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa91265
    Clone ID Related Accession (Same CDS sequence) XM_015464539.1 , XM_015472514.1
    Accession Version XM_015472514.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 9048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein prune homolog 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104102.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAGGA TTCCACAGGC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCAGA AGAGGAGCGG
    GAGGACAACC GGCTGTGGAG GACCGTGGTG ATCGGAGAAC AGGAGCAGCG GATTGACATG
    AAGGTCATCG AGCCCTATAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GATGGCCTAA ATGCCATCAT TGTGTTTGCC GCCTGTTTTC TGCCAGACAG CAGTCGGGCG
    GACTACCACT ATGTCATGGA AAATCTTTTC CTATATGTAA TAAGTACTTT AGAGTTGATG
    GTAGCTGAAG ACTATATGAT TGTGTACTTG AATGGTGCAA CCCCAAGACG GAAGATGCCA
    GGGTTAGGCT GGATGAAGAA ATGTTACCAG ATGATTGACA GACGGTTGAG GAAGAATTTA
    AAATCATTCA TCATTGTTCA TCCATCTTGG TTCATCCGAA CAATCCTTGC AGTTACACGA
    CCTTTTATAA GTTCCAAATT CAGCAGTAAA ATTAAATACG TCAGTAGTTT AGCAGAACTC
    AGTGGACTGA TCCCAATGGA TTGCATCCAT ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GATCTGAGGG AAGCATCTGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TAATCTGAAG CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015328000.1
    CDS190..9237
    Translation

    Target ORF information:

    RefSeq Version XM_015472514.1
    Organism Bos taurus(cattle)
    Definition Bos taurus prune homolog 2 (Drosophila) (PRUNE2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472514.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    ATGGAAGAAT TTTTGCAGCG CGCCAAGTCT AAATTGAATC GAAGCAAACG TTTGGAGAAG 
    GTCCATGTGG TTATTGGACA CAAATCCTGT GATTTGGATT CTCTCATTTC TGCCTTCACA
    TATGCTTACT TTCTAGACAA GGTCAGCCCA CCAGGGGTTC TCTGTTTGCC CGTGCTGAAC
    ATACCAAGAA CTGAATTCAA TTACTTCACT GAAACAAGGT TTATTTTAGA AGAGCTAAAT
    ATCTCTGAAT CATCCCACAT ATTCCGAGAT GAAATTAATC TGCATCAGCT AAATAATGAA
    GGGAAGTTAT CTCTAACACT TGTTGGCAGC AACGTACTGG CAAGTGAGGA CAAAACTTTA
    GAATCAGCAG TTGTCAAGGT CATTAATCCG GTGGAGCAGG GCGACACCGG CTTAGAGTTC
    CGAGAGTCTT CGTCGTCTCT GGTGGTGAAA GAGATCCTCC AGGAGGCTCC CGAGCTCATC
    ACCGAGCAGC TGGCTCATCT GCTCAGAGGT AGCATCCTTT TTAAGTGGAT GGCCATGGAG
    CCCAAGAAGA TCTCAGAAAA GCAGGAGGAA ATTCTTTCTA TCCTGGAAGA AAAATTTCCC
    AGCTTACCTC CAAGAGAGGA CATTATCAAC ATCCTGCAGG AGACCCAGTC CAGTGCTCAG
    GGTTTAGGTA TTGAACAGAC AATGCTGAAG AATCTAAAGG AACTTTCAGA TGGAGAAATA
    AAAGTGGCCA TTAGCACTGT GAACATGACC CTGGAGAACT GTACATTTCA CAGCAATATC
    ACCAGTGATT TGAAAGCATT CACAGAAAAG TTTGGTTTTG ATGTCCTCAT CCTCCTGGCC
    AGTTACCTGT CAGAGGAGCA GCAGTCCAGA CAACAGATCG CTGTGTATTC TGAGAACCTA
    GAGCTGTGCA GTCAGATCTG CTGTGAACTG GAAGAGTGCC AGAACCCTTG CCTGGAGCTG
    GAACCCTTTG AGTGTGGCTG TGATGAGATC CTGGTGTACC AGCAGGAGAA CCCTTCAGTG
    ACTTGTGATC AGGTGGTTCT CCTTGTCAAG GAAGTCATCA ACAGGAGGTG TCCGGAGATG
    GCCTCCAACA GCCGGACATC CTCCACAGAA GCGGTGGCAG GCAGTGCCCC CCTCTCCCAG
    GGATCTTCAG GGATTATGGA GTTGTATGGT TCTGATGTAG AGCCACAGCC CAGCTCCGTG
    AATTTCTTGG AAAATCCTCC TGATCTCGGC GATTCTAACC AGGCCCAGGT GGATGTCAAT
    GCGGACCTGG TGAGCCCAGA CAGTGGGCTG GCCACCATCA GGAGCAGCCG CTCATCCAAG
    GAGAGCTCCG TGTTCCTCAG TGACGACAGC CCTGTGGGAG AAGGGGCGGG GCCTCACCAC
    AGCCTCCTCC CAGGGTTTGA CTCCTACAGT CCCATCCCCG AAGGAGCAGT AGCGGAGGAG
    CATGCCCGCT CTGGAGAACA TGGTCAATCA TTCGACCTTT TCAACTTTGA CCCAGCACCC
    AGGGTTTCTG GGCAGTCTCA ACCATCCTCC CGTTCTGCAG ACTACTCCCC AACAGATGAC
    TTCTTCCCCA ACAGCGATTC CTCAGAAGGA CAGCTCACCA TGGGGTCTAA AGAACTTGAC
    AGGAGGGGGA TGGACATGCC TAATTATTCA TCCAGTTCTC TCTTGTCAGG GGCTGGCAAA
    GAGAGCCTCG TGGAATTTGA TGAGGAGTTT GTCCAGAGAC AAGAAAGTCC CGGGGATAAT
    TCTGAAAGAA ATTTGAGCCT GACAGGTTTT ATGCAAGATG AATCTCCTTC TCCAGAAAGT
    CTAAAAACTG TTGGAAAGAG GATCCCACCA ACACCTATGA ACAGCTTTGT GGAAAGTTCA
    CCATCCACTG AAGAACCAGC TCTGCTCTGT TCAGAAGACG TGACCCCCAA GACAGTCAAC
    ACAGGCCACC CAGGGCCACC TCTAACCCGG GCGCGATGCA GCAGCTGGTG GGATGGTTTG
    GAAATCGACT CTAAAAACAT CGTGGATGCA TGGAGTTCCA GCAAACAGGA ATCTGTCTTC
    CAGAGCCCTG AGTCCTGGAA AGATCATAAG CCAAGCCCAG CTGATAGAAG AGCCTCAGAC
    TCTATATTTC AACCCAAGAA TCTCGACTTC CCAAAGTCAA GTCCCTGGGA GTCAGAATTT
    GGTCAATCTG GGCTGGGCAA CAAGAATATT CAAGACTCAG ATGAGAAAAA CTTCCAGTTT
    CAGGACATGC CCCCTGAGAA ATCACGTTTG CTGAATGTTT CTCCTCACGG AACAAACCAC
    CTGATAGAAG ACTTCGCTGC CCTGTGGCGT TCTGACCGCT CTCCCACAGC CATGCCTGAG
    CCGTGGGGAA ACCCCACAGA GGATGAGGAA CCAGCTGCTG CAGCGTCATT CCCAGCCTGG
    AGTGCATTTG GTGAAGAAGG TGATGCCAAA GCTTTGAAAA ATACTTGGAA TCTGCACCCA
    ACGAGCAACG AGACACCTTC TGTGAGAGAC CCAAACGAGT GGGCCATGGC CAAAAGTGGG
    ATTGCTTTCC CTTCAGAAGA CCTCGTGGAC AGTTCACCCA GTGAGGCAAA TAATGAAGCG
    GTTCAAAAAA TCTGGGGCAA GAAGAACCAT GACCCCAGGG ATGATATTCT CATATCTGGA
    AATCCCACAT CTGATCTGGA TCATCCATGG AATAGTTCTA AGCCAGCAAA GGACCATCAG
    AACAGTGTGG TGGACCCAAC AATTAGGGGC AAAATGTACA AAAATGTAGA TTCTTGGAAT
    CTTTTCGAGG AGAGTAACAA GGAAGGAGGT TCAGATGTCC CAGCACCTTG GGAGGACTCC
    TTCTTATCCT ATAAATGTTC TGACTACAGT GCATCCAACG TCAGGGAAGA CTCAATGCCC
    TCCCCCTTAG ATACCAACTA CTCCACCTCT GACTCTTATA CTTCACCAAC ATTGGCCAAA
    GAGGAGAAAG AAACCAAAGA CAAGCCACTC ACTAAAGAGG AGGGTTTTGA GTCACAAGAT
    GCTAATTGTA CTGCAGGGGG CGCTGACATG CCTCCTCAGT CCCCGCAGCA GCCACCAAGA
    AATCGAATGA GTTCAGGTCC TGGGAACCTG GAAATGTGGG CTTCATCTCA GGCAGATGGT
    GGCACAGAAA TAAATGCCAC CCATAGCCCA GATAAAGATC TACTCCAAGT AGAGCCCACG
    GATGATCAGA ATGTCTCCTT TGAGGATGAC ATGGGGGAAA GCAGCCAGTC CAGTTATGAC
    GACCCCAGCA TGATGCAGCT GTACAACGAG ACGAACCGAC AGCTCACACT TCTGCACAGC
    AGTGTTCACG CCCGGCAGGC AGCCCCTGAC AGCCTCGACA CGTGGAACAG AGTGATTCTG
    GAGGACACTC AGTCTACTGC AACCATCTCG GATATGGACA ATGACTTGGA CTGGGATGAC
    TGCAGTGCGG GCGTGGCCAT CACTGGTGAA GGTCAGGCAC AAGGGTACAT GGCGGAAGGT
    AGCGAACCTG AAACCCGATT TTCAGTGAGA CAGGTAGAAC CCTGGGGCGT GGAGTATCAG
    GAGGCAAATC AGTCAGACTG GGAACTCCCT GCCTCTTCTG AGCACAGCCT GGACGTGGCT
    CCTGATGAAT ACCACGTGCT GAATGAGAAA AGTGGGCAGC TGATCGCGAA TAGTATTTGG
    GATTCTGTCA TGAGAGATAA TGCCATGTCA TCATTAATAT TACCTAGCCC ATCATATGCT
    ACAGATACAG AACAGAGCAG ATCACCTCCT GAAACAACCA GCTCATTAGA AAAAATTCAG
    GATAGTCACA TTGCAGACGT GCTGGAAGCC TCTGAAGCCA GTGAGTCTGA AACAATCGAT
    CCTGACAAGC AGGACACATG CAGAGGAACC CTGCCAAGTG ATTCGGCATA TTTGGTGACC
    AGACTTGAGA ATCCAGAGCA TTTTGCATGC TCAGATCCCT GGAAGGGTCC GAGCTATGGA
    CAAAAGGAAA GTGAGAAGGA ATTCCACGGA GCTGCTGATA GGGAGAATTC CAGTGATGAG
    CTAATGGATG GAACCTCAGA ACCTTCTGGA AAAGGGCCAG GTAGCCAGGA ATGCCCATCC
    CCAGCTGCAT CTGATCATCA AGAAATCTCC AATGAACCAG GCAAAATCAG AAATTTTTCA
    GTGACTTCCA GTCCTCAGAC CAAGGAACCC CAGGAAGTAT TAGAGCAGGA GAAGGGGTCT
    TACACTCCAG ACCCCTGTGA TGTGCAAACA GAGGTGTCCG AAGATCACTT GCCAGCAAGA
    GAGACCTGTG AAGAGCTCCT CATAAGCCCC AGAAGAAATT CAAGTGAAAC TACTGAAATT
    CCAGGTAGGC TGAATTCCAC AAAAAGTGTG TGTCTACCTC AAGTGGATGT GGAGAAAGCT
    GAAGAATGTG ACATTCTGGA GCCAGAGAAA ATTGACCAAG AGCCACCTGA TGAATGTCCC
    CAAGGCCTTG ACTCTTGGGA TGGCCCTAAC GACAGTGATG TGCTGGTCAG TGGTGCAAGT
    TCTGAGATAA CTATGAATCT CGAAGTTAGA AATACTGAAA ACAGCCCTCC AAGAGCCAAT
    TTATCAGGAA ATGATGACAG AGATAGTATT TCTAGTGAGT ATACACTTTC AAGTGCATCT
    AGTCCTGACA TAAATGATTC TTCAGCTACA TTGCCTTCCT GGGACCAAGG ACTCAATACT
    GGGCATCAGA AGGAAAATCA AAATGATTGG AGTACACAGA ATCGCCAAGA ATCTGAACTA
    ATGACTACTG ATGGTCATAC AGAAGTAGTT GCTGAAATGC AGGGTTTGGA GAAAAACAGA
    ATGGACAGGT TTGAAAGGAG CTTAGATCAC AAGGTTCCTA AATTTTTAGA GTTTTGGGGT
    GACTCAATAG ATGGTGATTC CTTTTCTTCT TTATCCAGCC CAGAAACAGG TAGATATTCT
    GAATATTCAG ATGCGTATCA GGAAAGAAAG CTAGTGGTCA GCCATCAGGA GAAAAATGAA
    CGTGATCTTC CTGAAACTGT ACAGCCAGAG GACACCAGCT TCATTTCAAC AAGCTCAGGT
    TCTGATGATG GTGAAGGCTA TGAAGAGCCA GTGGAGAAAG AGATCCATTC GGATACTTGT
    CAAGTGGCTC CGAGTGAACT CAGAGCTTGG GATTCATTGA ATAAATCTAA TAAGTCCTTG
    GCCACAGCAG ATCCTGAATC TGAGCAATGT TCTGTCTATA GAGGCAGTCC AGAGCAAGCA
    GAGAATGAGA ATAAGTCAAA CCCATCCTGT GATGATCAAC AAAGCAGCCC TGATCACTGG
    AATTTCTCAC CAGTAAGGAA GACTGAATTA CAGGTAACAT CAGTCGATAA GGAGACAAGG
    TCTTCTCCAG AAATAGGGAG GACAGGAGAT ATCATATGGC AAATATCTCC CAAAGCTGAA
    CCAAATAATG AAAATTACTC TAAATTGGAA ATGGCTGACT TCTCAACTGA GAGCACGGAG
    TGGTGGAAGC CCTCCCTACA GGAAGGACGA CCAATCGAGA GTGCATTTGA AGGAGAATTA
    TCTAACTCAA GTGCTGTGCT AGAAATGAAT TCCTCAGTGT GCGAAAACAT GGGTCCTTGG
    GGAGTGCCCA TTCAGGGTGA TACTGAATCC CTGGCACTAC ATGGTACCAA TCCTTTCAAT
    GATAACCATC AGTCACCCTT TCTAGATCAT AATGGGGAGA ATGCCCACGA GCAGCTTTGG
    AACATTCAAC CAAGGCAGCC AGACCCAGAA GCTGATCAGC TTAACCAACT TGTAATATTG
    GAACAAATTA AAGACACAGA TTCCAGAGAA CAAACCTTCA TGTCTTCTGC CGGTGACGAA
    CTCTCCTCTG AAGTGCCTAC GCAAGAACAG TGTCAGAATT CAGTGCTGTC TGCCTGGGAC
    CAGCCAGACT CGTCTTGTAC TTACGCAGAT GAAAATGGAT GCATTGTATC TGGTGTAGCA
    ACAACTGAGT GTCAAGAAAC AAATTGGTGG GAAGAAGAAA AATCACACCC AAGTGAAATG
    ACAAATTCAA ACATGACCTC TGAAGATTTC CCTGCTGTCA GTTCTCCCAC CCAGATGATG
    AACAAGTCAG GCTCTGAATG GAGTAACTCT ACCCCTACTG AGGGCCCCCA GGGCACGTTT
    GTTCCAGATA TTTTACACGG CAACTTCCAA GAGGGTGGGC ACCTGGCCTC ATCTTCGCCC
    GACTTGTGGA TGGGTGTGAA ACAGCCCTTC AGTGTGAAGA CAGATGGTGA GAATCCTGAT
    ATACTGACTC ACTGTGACCA CGACAGCAAT TCCAAGGCTT CCAACAGCCC TGATGTTTGT
    CATGATTATG AAGTAAAGCC AGAGACTAAG CAGCACATCG ATGCTGGGCT GGGACCTGAA
    AGGGAAGCCA GTGAGTTATA TCTCACTGAG CCAGAGATAG ATGAAGAATC CAGACAGGAG
    CCTGGGCAGG AATTTGTCCC TTATACTTCA GAACTGTCCT CTGAAAATGT CATTCCACTG
    CCTCCAGTCA GCGAGCAGAC AAACACAAAT GACTCATCTC AGCCTTCCTC TCACGAGTGT
    TTTCCTGAAC CTTCTGAAAT TAATGGTGAA AACAATGCAG GCTTAAAAGC ATCAGAACAA
    GGAGCCAACC CAGATATGGC ACCAGTTTTG GAACCTGTTG ACAGAACAAT CTCAATGATT
    GAAAATGTGG AAACTGGTAT TTGTGTAACT TACCAAGAGA CAACTCTGGA CGACAGTAGA
    TCTTCGATAT CAGAAGACTT TCTTCCTCAG AGGCAGACCG ATGCAGGGGA CTTGTTGGAC
    CATGAGCAGC CCTCTGTTAC TGAGAATCCT GCCACGGTTC CTGATGCTTT GCTAGCCTCG
    GACACTTGTC TGGATGTCAG TGAAGCTGCC TTCGACCACA GTCTCAGCGA TGCCTCGGGT
    CTCAACACAT CCACGGGAAC AATAGATGAC ATGAGTAAGT TGACATTATC AGAAGGCATT
    CCTGAAACGC CATTTGATGG GGAGACAGGG AAGCATGATA TCTGTTCCTC TGAAGCCTCG
    TGGGGTGACT TTGAGTATGA TGTCATGGGC CAAAATATTG ATGAAGACTT AATGAAAGAG
    CCTGAACACT TTGTTTATGG TGTTGACCCT CCCTTGGAAG AAGATGCCCT GAAGCATGAG
    TTGGCACCTT ACACACCTCC CTTTGATTTA TCCTATCTCA CGGAACCTAC TCCTGGCATC
    AAAACCACAG AGGAATCTGG GACTCCAGAG GATGAGTCTC TGGGGAGCGA AGCAGCAGAG
    ATACTGCTTT CTGCCCTTCC TGATGAAAGA AACCAGGAAA ACCATGCTGA GACCACAGAC
    AGACAGCCCG AAGGCCAGCC AGTTGTACTG CACATTCACG AAGACCCTGA GTCTGTTCCT
    TTGCCTGTCA GAATTGATGC CAACCCTGAG TTGTCTCCAT CACACATTGA CTGGGAAGTA
    GAAACAGATC GTTCAGATTC ACCAGCAGGT GGAGACATAG GACCACCAAG TGGTGCCAGT
    GAGGGAATAT TAGAGTTGGA AGAAGAAAAA ATAATTCCTA CCAAAGAGCC TGAGCAGGTA
    AAATCAGAAC ATGGGGAGGA AAGGAACACT GAGAAGAAAG AAGATCGTCA TGCATTGCAC
    ATGGATTACA TACTTGTAAA CCATGAAGAA AATTCACTGC CAGAGCCAGA GGCCTGTGAA
    GCTAGAGAAA TCACATTGGA ATTAGAAGAG CTTCACGTAG ATTCCGAGGA AAAAGGGCTG
    CCAGAAACTC AGTTAGCCAG CTCCCCGGAC ACAGGTCAAC CAGCTTCCTT AAATGAAAGA
    AATCATCTAT CTACAGAAAG AATGTCTTCC AAAGATGATA AGAGATCATC TTCTGAAAGC
    CCTGGGCAAG ACCAGAGTTG GATGGTCTTG GGCCGTAGTG AGGTTAGTGA TCCATCATCA
    GAGACCATGG ACTCAGGACC TGGATGGTCT GGTGAGGCTG TGGAGCCAGC CTCTGATCAC
    AGCTTGGGCC AGAGCCCCCA AATACAGATT CTGGAAGAAA TGAAGCCTCT AGAATCTTTA
    GCACTAGGGG AAGCCTCTGA TCTGGGCAGC CAATCACGGA GGAGCAAGAG CCGAGGCAAA
    GCTGGCCCTG ATGCAGTTAT GTTGCAGCCT GTCACCCATG ACAATGAATG GGAAATGCTT
    TCACCACAAA ACATAATCCC TGAAATGGAA ATGGAGGAGG AGACAGAGTT TCTTGAGCCC
    AGAACAAGGA AACCAAGACG GAATGGACTG CTGTCAGAGG ATGTGGGAAT GGACATCCAC
    TCTGAGGAGG GGATGCTGAG TCCCATTGCT GCAGACATGA GGCCAGAGGA TTCCACAGGC
    AACAAAGATT CTGGCCAAGA GTCAGAGTCT ATTCCAGAAT ATACGGCAGA AGAGGAGCGG
    GAGGACAACC GGCTGTGGAG GACCGTGGTG ATCGGAGAAC AGGAGCAGCG GATTGACATG
    AAGGTCATCG AGCCCTATAG GAGAGTCATT TCTCACGGAG GAGACTCAGG ATACTATGGG
    GATGGCCTAA ATGCCATCAT TGTGTTTGCC GCCTGTTTTC TGCCAGACAG CAGTCGGGCG
    GACTACCACT ATGTCATGGA AAATCTTTTC CTATATGTAA TAAGTACTTT AGAGTTGATG
    GTAGCTGAAG ACTATATGAT TGTGTACTTG AATGGTGCAA CCCCAAGACG GAAGATGCCA
    GGGTTAGGCT GGATGAAGAA ATGTTACCAG ATGATTGACA GACGGTTGAG GAAGAATTTA
    AAATCATTCA TCATTGTTCA TCCATCTTGG TTCATCCGAA CAATCCTTGC AGTTACACGA
    CCTTTTATAA GTTCCAAATT CAGCAGTAAA ATTAAATACG TCAGTAGTTT AGCAGAACTC
    AGTGGACTGA TCCCAATGGA TTGCATCCAT ATTCCAGAGA GCATCATCAA ACTGGATGAA
    GATCTGAGGG AAGCATCTGA AGCAGCTAAA ACTAGCTGCC TTTACAATGA TCCAGAAATG
    TCTTCTATGG AGAAGGATAT TAATCTGAAG CTGAAAGAAA AGCCCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.