SCN4A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN4A
Entrez Gene ID	517426
Full Name	sodium voltage-gated channel alpha subunit 4
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 4 Names sodium channel protein type 4 subunit alpha sodium channel, voltage-gated, type IV, alpha subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003587460.5	XP_003587508.2	sodium channel protein type 4 subunit alpha isoform X1
XM_015468204.1	XP_015323690.1	sodium channel protein type 4 subunit alpha isoform X2
XM_015458911.1	XP_015314397.1	sodium channel protein type 4 subunit alpha isoform X1
XM_005220988.3	XP_005221045.1	sodium channel protein type 4 subunit alpha isoform X2
XM_003583612.4	XP_003583660.1	sodium channel protein type 4 subunit alpha isoform X1
XM_015468203.1	XP_015323689.1	sodium channel protein type 4 subunit alpha isoform X1
XM_003587460.4	XP_003587508.1	sodium channel protein type 4 subunit alpha isoform X1
XM_005220988.4	XP_005221045.2	sodium channel protein type 4 subunit alpha isoform X2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SCN4A	XP_853396.1
Rattus norvegicus (Norway rat)	Scn4a	NP_037310.1
Pan troglodytes (chimpanzee)	SCN4A	XP_003315741.1
	SCN4A	XP_001116451.1
Mus musculus (house mouse)	Scn4a	NP_573462.2
Xenopus tropicalis (tropical clawed frog)	LOC100492371	XP_002933087.2
Homo sapiens (human)	SCN4A	NP_000325.4
Bos taurus (cattle)	SCN4A	XP_003587508.1
Danio rerio (zebrafish)	scn4aa	NP_001034914.1
Gallus gallus (chicken)	SCN4A	XP_004948566.1

Gene Ontology

The following SCN4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa226935	XM_003587460.5 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa226936	XM_005220988.4 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa30202	XM_015458911.1 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa30202	XM_003583612.4 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa30202	XM_015468203.1 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa30202	XM_003587460.4 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa30203	XM_015468204.1 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa30203	XM_005220988.3 Latest version!	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN4A ( XM_003587460.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587508.2 Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X1, mRNA.

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CloneID	OBa226935
Clone ID Related Accession (Same CDS sequence)	XM_003587460.5
Accession Version	XM_003587460.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003587460.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT CGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG TGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGCCT GGCTGACGGC 
CCTCCCCCCA GCATCGAGTT GGATCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTGTCA 
CCCACACTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTT GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT CGAGATCACC ACGTCGGCCG GCTGGGATGG GCTTCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGCGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCCGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CAGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587508.2
CDS	563..6067
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYSPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_003587460.5
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587460.5

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT CGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG TGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGCCT GGCTGACGGC 
CCTCCCCCCA GCATCGAGTT GGATCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGCGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTGTCA 
CCCACACTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTT GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT CGAGATCACC ACGTCGGCCG GCTGGGATGG GCTTCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGCGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCCGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CAGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_005220988.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221045.2 Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X2, mRNA.

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CloneID	OBa226936
Clone ID Related Accession (Same CDS sequence)	XM_005220988.4
Accession Version	XM_005220988.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220988.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT CGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG TGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGCCT GGCTGACGGC 
CCTCCCCCCA GCATCGAGTT GGATCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GCGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTGTCACCC 
ACACTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTTGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTCGA GATCACCACG TCGGCCGGCT GGGATGGGCT TCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GCGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC CGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACAG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221045.2
CDS	564..6065
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYSPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_005220988.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 4 (SCN4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220988.4

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT CGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG TGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGCCT GGCTGACGGC 
CCTCCCCCCA GCATCGAGTT GGATCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GCGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTGTCACCC 
ACACTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTTGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTCGA GATCACCACG TCGGCCGGCT GGGATGGGCT TCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GCGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC CGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACAG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_015458911.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314397.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.

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CloneID	OBa30202
Clone ID Related Accession (Same CDS sequence)	XM_015458911.1 , XM_003583612.4 , XM_015468203.1 , XM_003587460.4
Accession Version	XM_015458911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314397.1
CDS	1..5505
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_015458911.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015458911.1

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_003583612.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003583660.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30202
Clone ID Related Accession (Same CDS sequence)	XM_015458911.1 , XM_003583612.4 , XM_015468203.1 , XM_003587460.4
Accession Version	XM_003583612.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003583612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003583660.1
CDS	594..6098
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_003583612.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003583612.4

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_015468203.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323689.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.

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CloneID	OBa30202
Clone ID Related Accession (Same CDS sequence)	XM_015458911.1 , XM_003583612.4 , XM_015468203.1 , XM_003587460.4
Accession Version	XM_015468203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323689.1
CDS	1..5505
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_015468203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468203.1

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_003587460.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_003587508.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.

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CloneID	OBa30202
Clone ID Related Accession (Same CDS sequence)	XM_015458911.1 , XM_003583612.4 , XM_015468203.1 , XM_003587460.4
Accession Version	XM_003587460.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5505bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003587460.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003587508.1
CDS	594..6098
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_003587460.4
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003587460.4

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGAAG CCGCTGCAGC CCCTGGATGG GAACTCCTCC 
GTCTGCAGCA CTGCTGACTA CAAGCCCCCC GAGGACGACC CCGAGGAACA GGCCGAGGAG 
AACCCTGATG GGGAGCAGCC TGAGGAGTGC TTCACCGAGG CCTGCGTGCA GCGCTTTCCC 
TTCCTCTACG TGGACATCTC CCAGGGTCGA GGGAAGAAGT GGTGGACCCT CCGCAGGGCC 
TGCTTCAAGA TCGTTGAGCA CAACTGGTTT GAGACCTTCA TTGTCTTCAT GATCCTGCTC 
AGCAGTGGGG CCTTGGCCTT CGAGGACATC CACATTGAGC AGCGGCGAGT CATCCGCACC 
ATCCTGGAGT ACGCCGACAA GGTCTTCACC TACATCTTCA TCCTGGAGAT GCTACTCAAG 
TGGGTGGCCT ACGGCTTCAA GGTGTACTTC ACCAATGCCT GGTGCTGGCT CGACTTCCTC 
ATCGTGGACG TCTCCATCAT CAGCCTGGTG GCCAACTGGC TGGGCTACTC GGAGCTGGGA 
CCCATCAAAT CCCTGCGGAC GCTGCGGGCC CTGCGCCCCC TGAGGGCACT GTCTCGATTC 
GAGGGCATGA GGGTGGTGGT GAATGCCCTC CTGGGAGCCA TCCCCTCCAT CATGAACGTG 
CTGCTCGTCT GCCTCATCTT CTGGCTCATC TTCAGCATCA TGGGTGTCAA CCTGTTTGCC 
GGCAAGTTCT ACTACTGCAT CAACACCACC ACCTCCGAGA GATTCGACAT CACCGAGGTC 
AACAACAAGT CCGAGTGCGA GAGCCTGATG CACACGGGCC AGGTCCGCTG GCTCAACGTC 
AAGGTCAACT ACGACAACGT GGGCCTCGGC TACCTCTCGC TCTTGCAGGT GGCCACCTTC 
AAGGGCTGGA TGGACATCAT GTATGCTGCC GTGGACTCCC GGGAGAAGGA AGAGCAGCCA 
CAGTATGAGG TGAACCTCTA CATGTACCTC TATTTCGTCA TCTTCATCAT CTTCGGCTCC 
TTCTTCACCC TCAACCTCTT CATTGGCGTC ATCATCGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GGAAAGACAT CTTCATGACA GAGGAACAGA AGAAATACTA TAACGCCATG 
AAGAAGCTTG GCTCCAAGAA GCCTCAGAAG CCAATTCCCC GGCCCCAGAA CAAGGTCCAG 
GGCATGGTGT ACGACCTGGT GACCAAGCAG GCGTTCGACA TCACCATCAT GATCCTCATT 
TGCCTCAACA TGGTCACCAT GATGGTGGAG ACGGACGACC AGAGCCAGCT CAAGGTGGAC 
ATCCTGTACA ACATCAACAT GGTCTTCATC ATCATCTTCA CGGGCGAGTG CGTGCTCAAG 
ATGCTGGCCC TGCGCCAGTA CTACTTCACC GTCGGCTGGA ATATCTTCGA CTTTGTGGTC 
GTCATCCTGT CCATTGTGGG CCTGGCGCTC TCCGACCTGA TCCAGAAATA CTTCGTCTCA 
CCCACGCTGT TCCGTGTGAT CCGCCTGGCG AGGATCGGGC GGGTCCTGCG GCTGATCCGC 
GGGGCCAAGG GCATCCGGAC GCTGCTCTTC GCGCTCATGA TGTCGCTGCC CGCCCTCTTC 
AACATCGGCC TCCTCCTCTT CCTGGTCATG TTCATCTACT CCATCTTCGG CATGTCCAAC 
TTCGCCTACG TCAAGAAGGA GTCGGGCATC GACGACATGT TTAACTTCGA GACCTTTGGC 
AACAGCATCA TCTGCCTCTT TGAGATCACC ACGTCGGCCG GCTGGGATGG GCTCCTCAAC 
CCCATCCTCA ACAGCGGGCC CCCTGACTGT GACCCCAACC TGGAGAACCC GGGCACCAGC 
ATCCGGGGCG ACTGTGGCAA CCCCTCCATC GGCATCTGCT TCTTCTGCAG CTACATCATC 
ATTTCCTTCC TCATCGTGGT CAACATGTAC ATCGCCATCA TCCTGGAGAA CTTCAACGTG 
GCCACGGAGG AGAGCAGCGA GCCTCTTGGG GAGGATGACT TCGAGATGTT CTACGAGACA 
TGGGAGAAGT TCGACCCCGA CGCCACGCAG TTCATCGACT ACAGCCGCCT CTCTGACTTC 
GTGGACACCC TGCAGGAGCC GCTGAGGATC GCCAAGCCCA ACAAGATCAA GCTCATCACG 
ATGGACCTGC CCATGGTGCC GGGGGACAAG ATCCACTGCC TGGACATCCT CTTTGCCCTG 
ACCAAAGAGG TCCTGGGTGA CTCCGGGGAG ATGGACGCCC TCAAGGAGAC CATGGAGGAG 
AAGTTCATGG CCGCCAACCC CTCCAAGGTC TCCTATGAGC CCATCACCAC CACCCTCAAG 
AGGAAGCACG AAGAAGTGTG CGCCATCAAG ATCCAGAGGG CCTACCGCCG GCATCTGCTG 
CAGCGCTCCG TGAAGCAGGC CTCTTACATG TACCGCCACA ACCATGACGG CGGCAGGGGC 
GGGGCCCCCG AGAAGGAGGG GCTGATCGCT GACACCATGA GCAAGGTGTA CGGCCCCGAC 
AACGGGAACA GCGGTGTGCC GAGCAAAGAG GAGGAGAGGG GCTCCACAGG GGACCCCGGA 
CCTGCCATGG GGCTCAGGCC CATCAGCCCC TCTGACGCAC CCCTCCCTCC CGCCCCACCC 
CTGGGGCAGA CGGTGCGCCC AGGGGTCAAA GAGTCTCTCG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_015468204.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015323690.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.

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CloneID	OBa30203
Clone ID Related Accession (Same CDS sequence)	XM_015468204.1 , XM_005220988.3
Accession Version	XM_015468204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646465.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTCTCACCC 
ACGCTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTCGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTTGA GATCACCACG TCGGCCGGCT GGGATGGGCT CCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GTGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC TGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACGG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015323690.1
CDS	597..6098
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_015468204.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468204.1

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTCTCACCC 
ACGCTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTCGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTTGA GATCACCACG TCGGCCGGCT GGGATGGGCT CCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GTGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC TGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACGG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN4A ( XM_005220988.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221045.1 Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.

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CloneID	OBa30203
Clone ID Related Accession (Same CDS sequence)	XM_015468204.1 , XM_005220988.3
Accession Version	XM_005220988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 4 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104497.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTCTCACCC 
ACGCTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTCGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTTGA GATCACCACG TCGGCCGGCT GGGATGGGCT CCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GTGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC TGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACGG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221045.1
CDS	597..6098
Translation	MAKASLPTVVPLGPESLRPFTRESLAAIERRVLEEEARQLRNKQMEIEETEQKPRSDMEAGKNLPLIYGDPPPELIGIPLEDLDPYYSDKKTFIVLNKGKSIFRFSATPALYILSPFSIVRRCAIKVLIHSLFSMFIMITILTNCVFMTMSDPPSWSKHVEYTFTGIYTFESLIKILARGFCIDDFTFLRDPWNWLDFSVIMMAYLTEFVDLGNISALRTFRVLRALKTITVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALVGLQLFMGNLRQKCVRWPPPFNDTNSTWYGNDTWYGNDTWGGNDTWGGNDTWGGQESWAGNYTFDWDAYISDEGNFYFLEGSNDALLCGNSSDAGHCPEGYECIKAGRNPNYGYTSYDTFSWAFLALFRLMTQDYWENLFQLTLRAAGKTYMIFFVVIIFLGSFYLINLILAVVAMAYAEQNEATLAEDQEKEEEFQQMLEKFKKQQEELEKAKAAQALEGGEVDGDPAHSKDCNGSLDTSAGEKGPPRPSCGTDSGISDAMEELEEAHQKCPPWWYKCSHKVLIWNCCTPWVKFKKIVNLIVMDPFVDLGITICIVLNTLFMAMEHYPMTEHFDSVLNVGNLVFTGIFTAEMVLKLIAMDPYEYFQQGWNIFDSIIVTLSLVELGLANVQGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKECVCKIAADCNLPRWHMHDFFHSFLIVFRILCGEWIETMWDCMEVAGQAMCLTVFLMVMVIGNLVVLNLFLALLLSSFSADSLAASDEDGEMNNLQIAIARITWGIGFAKAFLVGLLHGKVLSPKVIMLSLGSIGEDGEAGEAPESAPEDEKKEPPPEEDGKDLKKDNHILNHVGLADGPPPSIELDHLNFINNPYLTIHVPIASEESDLEMPTEEETDTFSEPEDVKPLQPLDGNSSVCSTADYKPPEDDPEEQAEENPDGEQPEECFTEACVQRFPFLYVDISQGRGKKWWTLRRACFKIVEHNWFETFIVFMILLSSGALAFEDIHIEQRRVIRTILEYADKVFTYIFILEMLLKWVAYGFKVYFTNAWCWLDFLIVDVSIISLVANWLGYSELGPIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYYCINTTTSERFDITEVNNKSECESLMHTGQVRWLNVKVNYDNVGLGYLSLLQVATFKGWMDIMYAAVDSREKEEQPQYEVNLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGKDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPQNKVQGMVYDLVTKQAFDITIMILICLNMVTMMVETDDQSQLKVDILYNINMVFIIIFTGECVLKMLALRQYYFTVGWNIFDFVVVILSIVGLALSDLIQKYFVSPTLFRVIRLARIGRVLRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMSNFAYVKKESGIDDMFNFETFGNSIICLFEITTSAGWDGLLNPILNSGPPDCDPNLENPGTSIRGDCGNPSIGICFFCSYIIISFLIVVNMYIAIILENFNVATEESSEPLGEDDFEMFYETWEKFDPDATQFIDYSRLSDFVDTLQEPLRIAKPNKIKLITMDLPMVPGDKIHCLDILFALTKEVLGDSGEMDALKETMEEKFMAANPSKVSYEPITTTLKRKHEEVCAIKIQRAYRRHLLQRSVKQASYMYRHNHDGGRGGAPEKEGLIADTMSKVYGPDNGNSGVPSKEEERGSTGDPGPAMGLRPISPSDAPLPPAPPLGQTVRPGVKESLV

Target ORF information:

RefSeq Version	XM_005220988.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type IV alpha subunit (SCN4A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005220988.3

ORF Insert Sequence:

ATGGCCAAGG CGTCTCTGCC CACGGTGGTG CCTCTGGGCC CCGAGAGCCT ACGCCCCTTC 
ACCCGGGAGT CCCTGGCAGC CATAGAGCGC CGGGTGTTGG AGGAGGAGGC CCGGCAGTTG 
CGGAACAAGC AGATGGAGAT CGAGGAAACC GAGCAGAAGC CGCGCAGCGA CATGGAGGCT 
GGCAAGAACC TGCCGCTCAT CTACGGGGAC CCCCCGCCGG AGCTCATTGG CATCCCCCTG 
GAGGATCTGG ATCCCTACTA CAGCGACAAG AAGACCTTCA TCGTGCTCAA CAAGGGCAAG 
TCCATCTTCC GCTTCTCAGC CACTCCTGCT CTCTACATCC TGAGCCCCTT CAGCATCGTC 
AGACGCTGCG CCATCAAGGT GCTCATCCAC TCGCTGTTCA GCATGTTCAT TATGATCACC 
ATCCTGACCA ACTGCGTGTT CATGACCATG AGTGACCCGC CTTCCTGGTC CAAGCACGTG 
GAGTACACCT TCACAGGGAT CTACACCTTC GAGTCCCTCA TCAAGATCCT GGCCCGAGGC 
TTCTGCATCG ATGACTTCAC ATTCCTCCGG GATCCCTGGA ACTGGCTGGA CTTCAGCGTC 
ATCATGATGG CGTACCTGAC GGAATTTGTG GACTTGGGCA ACATCTCAGC GCTGAGAACC 
TTCCGGGTGC TGCGGGCCCT GAAAACCATC ACGGTCATCC CAGGGCTGAA GACCATCGTG 
GGGGCCCTGA TCCAGTCGGT AAAAAAACTC TCGGACGTGA TGATCCTCAC TGTCTTCTGC 
CTGAGCGTCT TTGCCCTGGT GGGGCTGCAG CTCTTCATGG GAAACCTGCG GCAAAAGTGT 
GTGCGCTGGC CCCCGCCCTT CAATGACACC AACTCCACGT GGTATGGCAA CGACACGTGG 
TACGGCAACG ACACCTGGGG CGGCAATGAC ACCTGGGGCG GCAACGACAC CTGGGGCGGC 
CAGGAGAGCT GGGCCGGCAA CTATACCTTT GACTGGGATG CCTACATCAG TGATGAAGGG 
AACTTCTATT TCCTGGAGGG CTCCAATGAC GCCCTGCTCT GTGGGAACAG CAGCGATGCT 
GGGCATTGCC CTGAAGGTTA TGAGTGCATC AAGGCCGGGC GGAACCCCAA CTACGGCTAC 
ACCAGCTACG ACACCTTCAG CTGGGCCTTC CTGGCTCTCT TCCGTCTCAT GACACAGGAC 
TATTGGGAGA ACCTCTTCCA GCTGACCCTT CGAGCAGCCG GCAAGACCTA CATGATCTTT 
TTCGTGGTCA TCATTTTCCT GGGCTCCTTC TACCTCATCA ACCTGATCCT GGCTGTGGTA 
GCCATGGCCT ACGCTGAGCA GAACGAGGCC ACCCTGGCCG AGGATCAGGA GAAAGAAGAG 
GAGTTTCAGC AGATGCTGGA GAAATTCAAA AAACAGCAGG AGGAGCTGGA GAAGGCCAAG 
GCCGCCCAGG CTCTGGAAGG TGGGGAGGTG GACGGGGACC CAGCACACAG CAAAGACTGC 
AACGGCAGCC TGGACACATC GGCGGGGGAG AAGGGGCCTC CGAGGCCAAG CTGCGGCACA 
GACAGCGGTA TTTCCGATGC CATGGAAGAG CTGGAAGAGG CCCACCAGAA GTGCCCACCA 
TGGTGGTACA AATGTTCCCA TAAAGTGCTG ATATGGAACT GCTGCACCCC GTGGGTGAAG 
TTCAAGAAGA TCGTCAACCT GATCGTCATG GACCCCTTCG TGGACCTGGG CATCACCATC 
TGCATCGTGC TCAACACCCT CTTCATGGCC ATGGAGCATT ACCCAATGAC AGAGCACTTT 
GACAGTGTGC TCAACGTGGG CAACCTGGTC TTCACAGGCA TCTTCACAGC TGAGATGGTG 
CTGAAGCTCA TTGCCATGGA CCCCTACGAG TACTTCCAGC AGGGCTGGAA CATCTTCGAC 
AGTATCATTG TCACCCTCAG CCTGGTGGAG CTGGGCCTGG CCAATGTGCA GGGGCTGTCG 
GTGCTCCGCT CCTTCCGTCT GCTGAGGGTC TTCAAGCTGG CCAAGTCATG GCCGACACTG 
AACATGCTTA TCAAGATCAT CGGCAACTCG GTGGGGGCAC TGGGCAACCT GACGCTGGTG 
CTGGCCATCA TTGTGTTCAT CTTCGCCGTG GTGGGCATGC AGCTGTTTGG CAAGAGCTAC 
AAGGAGTGCG TGTGCAAGAT CGCCGCGGAC TGCAACCTGC CCCGCTGGCA CATGCATGAC 
TTCTTCCACT CCTTTCTCAT CGTCTTCCGC ATCCTGTGCG GGGAGTGGAT CGAGACCATG 
TGGGACTGCA TGGAGGTGGC CGGCCAGGCC ATGTGCCTCA CTGTCTTCCT CATGGTCATG 
GTCATCGGCA ACCTGGTGGT CTTGAACCTG TTCTTGGCCC TGCTGCTCAG CTCGTTCAGT 
GCCGACAGCC TGGCGGCCTC GGATGAGGAT GGCGAGATGA ACAACCTGCA GATTGCCATC 
GCTCGCATCA CGTGGGGCAT TGGCTTCGCC AAAGCCTTCC TCGTGGGGCT GCTGCATGGC 
AAGGTGCTGA GCCCCAAGGT TATCATGCTC AGCCTTGGAA GCATCGGCGA GGATGGGGAG 
GCCGGCGAGG CTCCAGAAAG CGCCCCCGAG GATGAGAAGA AGGAGCCTCC ACCCGAGGAG 
GATGGCAAGG ACCTCAAGAA GGACAACCAC ATCCTGAACC ACGTGGGTCT GGCTGACGGC 
CCTCCCCCCA GCATTGAGTT GGACCACCTC AACTTCATCA ACAACCCCTA CCTGACCATC 
CACGTGCCCA TCGCCTCCGA GGAGTCTGAC CTGGAGATGC CCACAGAGGA GGAAACTGAC 
ACCTTCTCTG AGCCTGAGGA TGTCAAGCCG CTGCAGCCCC TGGATGGGAA CTCCTCCGTC 
TGCAGCACTG CTGACTACAA GCCCCCCGAG GACGACCCCG AGGAACAGGC CGAGGAGAAC 
CCTGATGGGG AGCAGCCTGA GGAGTGCTTC ACCGAGGCCT GCGTGCAGCG CTTTCCCTTC 
CTCTACGTGG ACATCTCCCA GGGTCGAGGG AAGAAGTGGT GGACCCTCCG CAGGGCCTGC 
TTCAAGATCG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TCTTCATGAT CCTGCTCAGC 
AGTGGGGCCT TGGCCTTCGA GGACATCCAC ATTGAGCAGC GGCGAGTCAT CCGCACCATC 
CTGGAGTACG CCGACAAGGT CTTCACCTAC ATCTTCATCC TGGAGATGCT ACTCAAGTGG 
GTGGCCTACG GCTTCAAGGT GTACTTCACC AATGCCTGGT GCTGGCTCGA CTTCCTCATC 
GTGGACGTCT CCATCATCAG CCTGGTGGCC AACTGGCTGG GCTACTCGGA GCTGGGACCC 
ATCAAATCCC TGCGGACGCT GCGGGCCCTG CGCCCCCTGA GGGCACTGTC TCGATTCGAG 
GGCATGAGGG TGGTGGTGAA TGCCCTCCTG GGAGCCATCC CCTCCATCAT GAACGTGCTG 
CTCGTCTGCC TCATCTTCTG GCTCATCTTC AGCATCATGG GTGTCAACCT GTTTGCCGGC 
AAGTTCTACT ACTGCATCAA CACCACCACC TCCGAGAGAT TCGACATCAC CGAGGTCAAC 
AACAAGTCCG AGTGCGAGAG CCTGATGCAC ACGGGCCAGG TCCGCTGGCT CAACGTCAAG 
GTCAACTACG ACAACGTGGG CCTCGGCTAC CTCTCGCTCT TGCAGGTGGC CACCTTCAAG 
GGCTGGATGG ACATCATGTA TGCTGCCGTG GACTCCCGGG AGAAGGAAGA GCAGCCACAG 
TATGAGGTGA ACCTCTACAT GTACCTCTAT TTCGTCATCT TCATCATCTT CGGCTCCTTC 
TTCACCCTCA ACCTCTTCAT TGGCGTCATC ATCGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGGA AAGACATCTT CATGACAGAG GAACAGAAGA AATACTATAA CGCCATGAAG 
AAGCTTGGCT CCAAGAAGCC TCAGAAGCCA ATTCCCCGGC CCCAGAACAA GGTCCAGGGC 
ATGGTGTACG ACCTGGTGAC CAAGCAGGCG TTCGACATCA CCATCATGAT CCTCATTTGC 
CTCAACATGG TCACCATGAT GGTGGAGACG GACGACCAGA GCCAGCTCAA GGTGGACATC 
CTGTACAACA TCAACATGGT CTTCATCATC ATCTTCACGG GCGAGTGCGT GCTCAAGATG 
CTGGCCCTGC GCCAGTACTA CTTCACCGTC GGCTGGAATA TCTTCGACTT TGTGGTCGTC 
ATCCTGTCCA TTGTGGGCCT GGCGCTCTCC GACCTGATCC AGAAATACTT CGTCTCACCC 
ACGCTGTTCC GTGTGATCCG CCTGGCGAGG ATCGGGCGGG TCCTGCGGCT GATCCGCGGG 
GCCAAGGGCA TCCGGACGCT GCTCTTCGCG CTCATGATGT CGCTGCCCGC CCTCTTCAAC 
ATCGGCCTCC TCCTCTTCCT GGTCATGTTC ATCTACTCCA TCTTCGGCAT GTCCAACTTC 
GCCTACGTCA AGAAGGAGTC GGGCATCGAC GACATGTTTA ACTTCGAGAC CTTTGGCAAC 
AGCATCATCT GCCTCTTTGA GATCACCACG TCGGCCGGCT GGGATGGGCT CCTCAACCCC 
ATCCTCAACA GCGGGCCCCC TGACTGTGAC CCCAACCTGG AGAACCCGGG CACCAGCATC 
CGGGGCGACT GTGGCAACCC CTCCATCGGC ATCTGCTTCT TCTGCAGCTA CATCATCATT 
TCCTTCCTCA TCGTGGTCAA CATGTACATC GCCATCATCC TGGAGAACTT CAACGTGGCC 
ACGGAGGAGA GCAGCGAGCC TCTTGGGGAG GATGACTTCG AGATGTTCTA CGAGACATGG 
GAGAAGTTCG ACCCCGACGC CACGCAGTTC ATCGACTACA GCCGCCTCTC TGACTTCGTG 
GACACCCTGC AGGAGCCGCT GAGGATCGCC AAGCCCAACA AGATCAAGCT CATCACGATG 
GACCTGCCCA TGGTGCCGGG GGACAAGATC CACTGCCTGG ACATCCTCTT TGCCCTGACC 
AAAGAGGTCC TGGGTGACTC CGGGGAGATG GACGCCCTCA AGGAGACCAT GGAGGAGAAG 
TTCATGGCCG CCAACCCCTC CAAGGTCTCC TATGAGCCCA TCACCACCAC CCTCAAGAGG 
AAGCACGAAG AAGTGTGCGC CATCAAGATC CAGAGGGCCT ACCGCCGGCA TCTGCTGCAG 
CGCTCCGTGA AGCAGGCCTC TTACATGTAC CGCCACAACC ATGACGGCGG CAGGGGCGGG 
GCCCCCGAGA AGGAGGGGCT GATCGCTGAC ACCATGAGCA AGGTGTACGG CCCCGACAAC 
GGGAACAGCG GTGTGCCGAG CAAAGAGGAG GAGAGGGGCT CCACAGGGGA CCCCGGACCT 
GCCATGGGGC TCAGGCCCAT CAGCCCCTCT GACGCACCCC TCCCTCCCGC CCCACCCCTG 
GGGCAGACGG TGCGCCCAGG GGTCAAAGAG TCTCTCGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SCN4A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

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