DENND5B cDNA ORF clone, Bos taurus(cattle)

The following DENND5B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DENND5B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa234371 XM_024991732.1
Latest version!
Bos taurus DENN domain containing 5B (DENND5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa37603 NM_001205488.1
Latest version!
Bos taurus DENN domain containing 5B (DENND5B), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa19593 XM_005206831.4
Latest version!
Bos taurus DENN domain containing 5B (DENND5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa90783 XM_015471173.1
Latest version!
Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90784 XM_015471174.1
Latest version!
Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90785 XM_015471175.1
Latest version!
Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa90786 XM_015471176.2
Latest version!
Bos taurus DENN domain containing 5B (DENND5B), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBa19593 XM_005206831.3 Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa90786 XM_015471176.1 Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa234371
    Clone ID Related Accession (Same CDS sequence) XM_024991732.1
    Accession Version XM_024991732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3912bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GGTGAACGCT TTAGGCAGAG GCTATCTGCT GCTGATATTG TGGCACCAGA ACCTCATGAT
    TTCTTTGCAG AAAGACCACA AGTTGCTACC CAGTCAATTG AGCAGCGACT GATGAAAATG
    GATCACACCG CAATCCACCC ACATCTACTT GATATGAAAA TTGGTCAAGG CAAATACGAG
    CAAGGATTTT TTCCAAAGTT ACAGTCTGAT GTCTTGGCAA CAGGACCAAC TAATAACAAT
    CGCTGGGTGA GTCGGAGTGC CACTGCACAG CGCAGGAAAG ACCGCCTTCG CCAGCATTCC
    GACCACGTTG GGCTGGACAA CGACTTGAGG GAGAAATACA TGCAAGAGGC ACGAAGTTTA
    GGAAAAAACC TGAGGCAACC CAAATTGTCA GACCTCTCTC CTGCGGTGAT TGCACAAACC
    AACTGGAAAT TTGTAGAAGG CTTATTAAAA GAATGTAGAA TGAAGACGAA ACGCATGTTG
    GTGGAGAAGA TGGGACACGA AGCAGTGGAA CTGGGCCATG GAGAAGCCAA CATAACTGGC
    TTGGAGGAGA ACACCCTGAT TGCCAGCCTC TGTGACCTGT TGGAAAGGAT ATGGAGCCAC
    GGCTTGCAAG TCAAGCAGGG GAAGTCAGCT TTGTGGTCAC ATTTAATTCA GTTTCAGGAC
    AGAGAAGAGA AACAAGAGCA CCTTGCAGAG TCACCAGTTG CCCTTGGACC AGAAAGAAGA
    AAATCTGACT CAGGAGTTAT GTTGCCAACC CTCAGGGTCT CTCTTATCCA AGACATGAGG
    CATATTCAAA ACATGAGTGA GATCAAGACT GATGTCGGAC GAGCTCGAGC ATGGATAAGA
    TTGTCTCTAG AAAAGAAGGT CTTGTCCCAG CATCTCAAGC AGTTGCTCTC TAACCAGCTG
    CTCACAAAGA AGCTTTATAA ACGTTATGCT TTTCTCCGTT GTGAAGAAGA AAGAGAGCAG
    TTTCTTTACC ATCTTCTTTC CCTCAATGCT GTGGACTATT TCTGCTTCAC CAGTGTCTTC
    ACCACTATCA TGATTCCATA TAGGTCAGTG ATCATTCCCA TCAAAAAGCT GAGCAACGCG
    ATCATCACGT CAAACCCTTG GATCTGTGTA TCAGGAGAAC TAGGAGACAC GGGAGTAATG
    CAGATTCCCA AAAACCTCCT TGAAATGACT TTTGAATGCC AGAATCTGGG GAAGCTGACG
    ACTGTCCAGA TCGGTCACGA TAACTCAGGA CTGTTAGCCA AATGGCTAGT GGATTGTGTC
    ATGGTCAGAA ATGAAATCAC AGGACATACA TATAGGTTCC CATGTGGGCG GTGGCTGGGG
    AAAGGCATTG ATGATGGAAG TCTGGAGAGA ATTCTCATTG GAGAATTGAT GACATCAGCA
    GCAGATGAGG ATCTAGTAAA GCAGTGTCGG ACTCCACCCC AGCAGAAATC ACCCACTGTG
    GCCAGGAGAC TGAGCATCAC ATCACTGACA GGAAAAACTA CCAAACCCAA TGCAGGACAG
    ATCCAAGAAG GAATTGGAGA AGCTGTGAAC AACATTGTGA AACATTTTCA CAAACCTGAA
    AAAGAGAGAG GAAGCCTCAC TGTGCTGCTG TGTGGAGAGA GCGGCTTAGT TGCGGCGCTT
    GAGCAAGTTT TCCACCATGG CTTCAAATCA GCTCGCATCT TTCACAAGAA TGTCTTCATC
    TGGGACTTCA TAGAGAGAGC AGTTGCTTAT TTTGAAACCA CTGACCAGAT TCTAGATAAT
    GAAGATGATG TCCTCATTCA GAAATCATCC TGCAAAACCT TCTGCCACTA TGTAAATGCT
    ATTAATACTG CACCCCGGAA TATTGGGAAG GATGGCAAAT TCCAGATCTT AGTTTGCCTT
    GGAACACGAG ATCGCCTGCT CCCACAGTGG ATTCCTCTGT TAGCCGAGTG TCCTGCCATC
    ACCCGAATGT ATGAGGAGAG CGCCCTCCTG CGAGATCACA TGACGGTCAA CTCCCTTATC
    CGCATTCTGC AGACCATCCA GGACTTCACC ATAGTCTTAG AAGGCTCGCT CATCAAAGGA
    GTGGATGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847500.1
    CDS287..4198
    Translation

    Target ORF information:

    RefSeq Version XM_024991732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5B (DENND5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GGTGAACGCT TTAGGCAGAG GCTATCTGCT GCTGATATTG TGGCACCAGA ACCTCATGAT
    TTCTTTGCAG AAAGACCACA AGTTGCTACC CAGTCAATTG AGCAGCGACT GATGAAAATG
    GATCACACCG CAATCCACCC ACATCTACTT GATATGAAAA TTGGTCAAGG CAAATACGAG
    CAAGGATTTT TTCCAAAGTT ACAGTCTGAT GTCTTGGCAA CAGGACCAAC TAATAACAAT
    CGCTGGGTGA GTCGGAGTGC CACTGCACAG CGCAGGAAAG ACCGCCTTCG CCAGCATTCC
    GACCACGTTG GGCTGGACAA CGACTTGAGG GAGAAATACA TGCAAGAGGC ACGAAGTTTA
    GGAAAAAACC TGAGGCAACC CAAATTGTCA GACCTCTCTC CTGCGGTGAT TGCACAAACC
    AACTGGAAAT TTGTAGAAGG CTTATTAAAA GAATGTAGAA TGAAGACGAA ACGCATGTTG
    GTGGAGAAGA TGGGACACGA AGCAGTGGAA CTGGGCCATG GAGAAGCCAA CATAACTGGC
    TTGGAGGAGA ACACCCTGAT TGCCAGCCTC TGTGACCTGT TGGAAAGGAT ATGGAGCCAC
    GGCTTGCAAG TCAAGCAGGG GAAGTCAGCT TTGTGGTCAC ATTTAATTCA GTTTCAGGAC
    AGAGAAGAGA AACAAGAGCA CCTTGCAGAG TCACCAGTTG CCCTTGGACC AGAAAGAAGA
    AAATCTGACT CAGGAGTTAT GTTGCCAACC CTCAGGGTCT CTCTTATCCA AGACATGAGG
    CATATTCAAA ACATGAGTGA GATCAAGACT GATGTCGGAC GAGCTCGAGC ATGGATAAGA
    TTGTCTCTAG AAAAGAAGGT CTTGTCCCAG CATCTCAAGC AGTTGCTCTC TAACCAGCTG
    CTCACAAAGA AGCTTTATAA ACGTTATGCT TTTCTCCGTT GTGAAGAAGA AAGAGAGCAG
    TTTCTTTACC ATCTTCTTTC CCTCAATGCT GTGGACTATT TCTGCTTCAC CAGTGTCTTC
    ACCACTATCA TGATTCCATA TAGGTCAGTG ATCATTCCCA TCAAAAAGCT GAGCAACGCG
    ATCATCACGT CAAACCCTTG GATCTGTGTA TCAGGAGAAC TAGGAGACAC GGGAGTAATG
    CAGATTCCCA AAAACCTCCT TGAAATGACT TTTGAATGCC AGAATCTGGG GAAGCTGACG
    ACTGTCCAGA TCGGTCACGA TAACTCAGGA CTGTTAGCCA AATGGCTAGT GGATTGTGTC
    ATGGTCAGAA ATGAAATCAC AGGACATACA TATAGGTTCC CATGTGGGCG GTGGCTGGGG
    AAAGGCATTG ATGATGGAAG TCTGGAGAGA ATTCTCATTG GAGAATTGAT GACATCAGCA
    GCAGATGAGG ATCTAGTAAA GCAGTGTCGG ACTCCACCCC AGCAGAAATC ACCCACTGTG
    GCCAGGAGAC TGAGCATCAC ATCACTGACA GGAAAAACTA CCAAACCCAA TGCAGGACAG
    ATCCAAGAAG GAATTGGAGA AGCTGTGAAC AACATTGTGA AACATTTTCA CAAACCTGAA
    AAAGAGAGAG GAAGCCTCAC TGTGCTGCTG TGTGGAGAGA GCGGCTTAGT TGCGGCGCTT
    GAGCAAGTTT TCCACCATGG CTTCAAATCA GCTCGCATCT TTCACAAGAA TGTCTTCATC
    TGGGACTTCA TAGAGAGAGC AGTTGCTTAT TTTGAAACCA CTGACCAGAT TCTAGATAAT
    GAAGATGATG TCCTCATTCA GAAATCATCC TGCAAAACCT TCTGCCACTA TGTAAATGCT
    ATTAATACTG CACCCCGGAA TATTGGGAAG GATGGCAAAT TCCAGATCTT AGTTTGCCTT
    GGAACACGAG ATCGCCTGCT CCCACAGTGG ATTCCTCTGT TAGCCGAGTG TCCTGCCATC
    ACCCGAATGT ATGAGGAGAG CGCCCTCCTG CGAGATCACA TGACGGTCAA CTCCCTTATC
    CGCATTCTGC AGACCATCCA GGACTTCACC ATAGTCTTAG AAGGCTCGCT CATCAAAGGA
    GTGGATGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa37603
    Clone ID Related Accession (Same CDS sequence) NM_001205488.1
    Accession Version NM_001205488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3825bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000005.1. On Apr 20, 2011 this sequence version replaced XM_001789332.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145431, SAMN03145432 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GCCCAGTCAA TTGAGCAGCG ACTGATGAAA ATGGATCACA CCGCAATCCA CCCACATCTA
    CTTGATATGA AAATTGGTCA AGGCAAATAC GAGCAAGGAT TTTTTCCAAA GTTACAGTCT
    GATGTCTTGG CAACAGGACC AACTAATAAC AATCGCTGGG TGAGTCGGAG TGCCACTGCA
    CAGCGCAGGA AAGACCGCCT TCGCCAGCAT TCCGACCACG TTGGGCTGGA CAACGACTTG
    AGGGAGAAAT ACATGCAAGA GGCACGAAGT TTAGGAAAAA ACCTGAGGCA ACCCAAATTG
    TCAGACCTCT CTCCTGCGGT GATTGCACAA ACCAACTGGA AATTTGTAGA AGGCTTATTA
    AAAGAATGTA GAATGAAGAC GAAACGCATG TTGGTGGAGA AGATGGGACA CGAAGCAGTG
    GAACTGGGCC ATGGAGAAGC CAACATAACT GGCTTGGAGG AGAACACCCT GATTGCCAGC
    CTCTGTGACC TGTTGGAAAG GATATGGAGC CACGGCTTGC AAGTCAAGCA GGGGAAGTCA
    GCTTTGTGGT CACATTTAAT TCAGTTTCAG GACAGAGAAG AGAAACAAGA GCACCTTGCA
    GAGTCACCAG TTGCCCTTGG ACCAGAAAGA AGAAAATCTG ACTCAGGAGT TATGTTGCCA
    ACCCTCAGGG TCTCTCTTAT CCAAGACATG AGGCATATTC AAAACATGAG TGAGATCAAG
    ACTGATGTCG GACGAGCTCG AGCATGGATA AGATTGTCTC TAGAAAAGAA GGTCTTGTCC
    CAGCATCTCA AGCAGTTGCT CTCTAACCAG CTGCTCACAA AGAAGCTTTA TAAACGTTAT
    GCTTTTCTCC GTTGTGAAGA AGAAAGAGAG CAGTTTCTTT ACCATCTTCT TTCCCTCAAT
    GCTGTGGACT ATTTCTGCTT CACCAGTGTC TTCACCACTA TCATGATTCC ATATAGGTCA
    GTGATCATTC CCATCAAAAA GCTGAGCAAC GCGATCATCA CGTCAAACCC TTGGATCTGT
    GTATCAGGAG AACTAGGAGA CACGGGAGTA ATGCAGATTC CCAAAAACCT CCTTGAAATG
    ACTTTTGAAT GCCAGAATCT GGGGAAGCTG ACGACTGTCC AGATCGGTCA CGATAACTCA
    GGACTGTTAG CCAAATGGCT AGTGGATTGT GTCATGGTCA GAAATGAAAT CACAGGACAT
    ACATATAGGT TCCCATGTGG GCGGTGGCTG GGGAAAGGCA TTGATGATGG AAGTCTGGAG
    AGAATTCTCA TTGGAGAATT GATGACATCA GCAGCAGATG AGGATCTAGT AAAGCAGTGT
    CGGACTCCAC CCCAGCAGAA ATCACCCACT GTGGCCAGGA GACTGAGCAT CACATCACTG
    ACAGGAAAAA CTACCAAACC CAATGCAGGA CAGATCCAAG AAGGAATTGG AGAAGCTGTG
    AACAACATTG TGAAACATTT TCACAAACCT GAAAAAGAGA GAGGAAGCCT CACTGTGCTG
    CTGTGTGGAG AGAGCGGCTT AGTTGCGGCG CTTGAGCAAG TTTTCCACCA TGGCTTCAAA
    TCAGCTCGCA TCTTTCACAA GAATGTCTTC ATCTGGGACT TCATAGAGAG AGCAGTTGCT
    TATTTTGAAA CCACTGACCA GATTCTAGAT AATGAAGATG ATGTCCTCAT TCAGAAATCA
    TCCTGCAAAA CCTTCTGCCA CTATGTAAAT GCTATTAATA CTGCACCCCG GAATATTGGG
    AAGGATGGCA AATTCCAGAT CTTAGTTTGC CTTGGAACAC GAGATCGCCT GCTCCCACAG
    TGGATTCCTC TGTTAGCCGA GTGTCCTGCC ATCACCCGAA TGTATGAGGA GAGCGCCCTC
    CTGCGAGATC ACATGACGGT CAACTCCCTT ATCCGCATTC TGCAGACCAT CCAGGACTTC
    ACCATAGTCT TAGAAGGCTC GCTCATCAAA GGAGTGGATG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192417.1
    CDS1..3825
    Translation

    Target ORF information:

    RefSeq Version NM_001205488.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5B (DENND5B), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GCCCAGTCAA TTGAGCAGCG ACTGATGAAA ATGGATCACA CCGCAATCCA CCCACATCTA
    CTTGATATGA AAATTGGTCA AGGCAAATAC GAGCAAGGAT TTTTTCCAAA GTTACAGTCT
    GATGTCTTGG CAACAGGACC AACTAATAAC AATCGCTGGG TGAGTCGGAG TGCCACTGCA
    CAGCGCAGGA AAGACCGCCT TCGCCAGCAT TCCGACCACG TTGGGCTGGA CAACGACTTG
    AGGGAGAAAT ACATGCAAGA GGCACGAAGT TTAGGAAAAA ACCTGAGGCA ACCCAAATTG
    TCAGACCTCT CTCCTGCGGT GATTGCACAA ACCAACTGGA AATTTGTAGA AGGCTTATTA
    AAAGAATGTA GAATGAAGAC GAAACGCATG TTGGTGGAGA AGATGGGACA CGAAGCAGTG
    GAACTGGGCC ATGGAGAAGC CAACATAACT GGCTTGGAGG AGAACACCCT GATTGCCAGC
    CTCTGTGACC TGTTGGAAAG GATATGGAGC CACGGCTTGC AAGTCAAGCA GGGGAAGTCA
    GCTTTGTGGT CACATTTAAT TCAGTTTCAG GACAGAGAAG AGAAACAAGA GCACCTTGCA
    GAGTCACCAG TTGCCCTTGG ACCAGAAAGA AGAAAATCTG ACTCAGGAGT TATGTTGCCA
    ACCCTCAGGG TCTCTCTTAT CCAAGACATG AGGCATATTC AAAACATGAG TGAGATCAAG
    ACTGATGTCG GACGAGCTCG AGCATGGATA AGATTGTCTC TAGAAAAGAA GGTCTTGTCC
    CAGCATCTCA AGCAGTTGCT CTCTAACCAG CTGCTCACAA AGAAGCTTTA TAAACGTTAT
    GCTTTTCTCC GTTGTGAAGA AGAAAGAGAG CAGTTTCTTT ACCATCTTCT TTCCCTCAAT
    GCTGTGGACT ATTTCTGCTT CACCAGTGTC TTCACCACTA TCATGATTCC ATATAGGTCA
    GTGATCATTC CCATCAAAAA GCTGAGCAAC GCGATCATCA CGTCAAACCC TTGGATCTGT
    GTATCAGGAG AACTAGGAGA CACGGGAGTA ATGCAGATTC CCAAAAACCT CCTTGAAATG
    ACTTTTGAAT GCCAGAATCT GGGGAAGCTG ACGACTGTCC AGATCGGTCA CGATAACTCA
    GGACTGTTAG CCAAATGGCT AGTGGATTGT GTCATGGTCA GAAATGAAAT CACAGGACAT
    ACATATAGGT TCCCATGTGG GCGGTGGCTG GGGAAAGGCA TTGATGATGG AAGTCTGGAG
    AGAATTCTCA TTGGAGAATT GATGACATCA GCAGCAGATG AGGATCTAGT AAAGCAGTGT
    CGGACTCCAC CCCAGCAGAA ATCACCCACT GTGGCCAGGA GACTGAGCAT CACATCACTG
    ACAGGAAAAA CTACCAAACC CAATGCAGGA CAGATCCAAG AAGGAATTGG AGAAGCTGTG
    AACAACATTG TGAAACATTT TCACAAACCT GAAAAAGAGA GAGGAAGCCT CACTGTGCTG
    CTGTGTGGAG AGAGCGGCTT AGTTGCGGCG CTTGAGCAAG TTTTCCACCA TGGCTTCAAA
    TCAGCTCGCA TCTTTCACAA GAATGTCTTC ATCTGGGACT TCATAGAGAG AGCAGTTGCT
    TATTTTGAAA CCACTGACCA GATTCTAGAT AATGAAGATG ATGTCCTCAT TCAGAAATCA
    TCCTGCAAAA CCTTCTGCCA CTATGTAAAT GCTATTAATA CTGCACCCCG GAATATTGGG
    AAGGATGGCA AATTCCAGAT CTTAGTTTGC CTTGGAACAC GAGATCGCCT GCTCCCACAG
    TGGATTCCTC TGTTAGCCGA GTGTCCTGCC ATCACCCGAA TGTATGAGGA GAGCGCCCTC
    CTGCGAGATC ACATGACGGT CAACTCCCTT ATCCGCATTC TGCAGACCAT CCAGGACTTC
    ACCATAGTCT TAGAAGGCTC GCTCATCAAA GGAGTGGATG TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19593
    Clone ID Related Accession (Same CDS sequence) XM_005206831.4 , XM_005206831.3
    Accession Version XM_005206831.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206831.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGAAATACAT GCAAGAGGCA CGAAGTTTAG GAAAAAACCT GAGGCAACCC
    AAATTGTCAG ACCTCTCTCC TGCGGTGATT GCACAAACCA ACTGGAAATT TGTAGAAGGC
    TTATTAAAAG AATGTAGAAT GAAGACGAAA CGCATGTTGG TGGAGAAGAT GGGACACGAA
    GCAGTGGAAC TGGGCCATGG AGAAGCCAAC ATAACTGGCT TGGAGGAGAA CACCCTGATT
    GCCAGCCTCT GTGACCTGTT GGAAAGGATA TGGAGCCACG GCTTGCAAGT CAAGCAGGGG
    AAGTCAGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAGAA ACAAGAGCAC
    CTTGCAGAGT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG
    TTGCCAACCC TCAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG
    ATCAAGACTG ATGTCGGACG AGCTCGAGCA TGGATAAGAT TGTCTCTAGA AAAGAAGGTC
    TTGTCCCAGC ATCTCAAGCA GTTGCTCTCT AACCAGCTGC TCACAAAGAA GCTTTATAAA
    CGTTATGCTT TTCTCCGTTG TGAAGAAGAA AGAGAGCAGT TTCTTTACCA TCTTCTTTCC
    CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTCTTCA CCACTATCAT GATTCCATAT
    AGGTCAGTGA TCATTCCCAT CAAAAAGCTG AGCAACGCGA TCATCACGTC AAACCCTTGG
    ATCTGTGTAT CAGGAGAACT AGGAGACACG GGAGTAATGC AGATTCCCAA AAACCTCCTT
    GAAATGACTT TTGAATGCCA GAATCTGGGG AAGCTGACGA CTGTCCAGAT CGGTCACGAT
    AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA
    GGACATACAT ATAGGTTCCC ATGTGGGCGG TGGCTGGGGA AAGGCATTGA TGATGGAAGT
    CTGGAGAGAA TTCTCATTGG AGAATTGATG ACATCAGCAG CAGATGAGGA TCTAGTAAAG
    CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACTGTGG CCAGGAGACT GAGCATCACA
    TCACTGACAG GAAAAACTAC CAAACCCAAT GCAGGACAGA TCCAAGAAGG AATTGGAGAA
    GCTGTGAACA ACATTGTGAA ACATTTTCAC AAACCTGAAA AAGAGAGAGG AAGCCTCACT
    GTGCTGCTGT GTGGAGAGAG CGGCTTAGTT GCGGCGCTTG AGCAAGTTTT CCACCATGGC
    TTCAAATCAG CTCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTCAT AGAGAGAGCA
    GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGATAATG AAGATGATGT CCTCATTCAG
    AAATCATCCT GCAAAACCTT CTGCCACTAT GTAAATGCTA TTAATACTGC ACCCCGGAAT
    ATTGGGAAGG ATGGCAAATT CCAGATCTTA GTTTGCCTTG GAACACGAGA TCGCCTGCTC
    CCACAGTGGA TTCCTCTGTT AGCCGAGTGT CCTGCCATCA CCCGAATGTA TGAGGAGAGC
    GCCCTCCTGC GAGATCACAT GACGGTCAAC TCCCTTATCC GCATTCTGCA GACCATCCAG
    GACTTCACCA TAGTCTTAGA AGGCTCGCTC ATCAAAGGAG TGGATGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206888.1
    CDS286..4176
    Translation

    Target ORF information:

    RefSeq Version XM_005206831.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5B (DENND5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206831.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGAAATACAT GCAAGAGGCA CGAAGTTTAG GAAAAAACCT GAGGCAACCC
    AAATTGTCAG ACCTCTCTCC TGCGGTGATT GCACAAACCA ACTGGAAATT TGTAGAAGGC
    TTATTAAAAG AATGTAGAAT GAAGACGAAA CGCATGTTGG TGGAGAAGAT GGGACACGAA
    GCAGTGGAAC TGGGCCATGG AGAAGCCAAC ATAACTGGCT TGGAGGAGAA CACCCTGATT
    GCCAGCCTCT GTGACCTGTT GGAAAGGATA TGGAGCCACG GCTTGCAAGT CAAGCAGGGG
    AAGTCAGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAGAA ACAAGAGCAC
    CTTGCAGAGT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG
    TTGCCAACCC TCAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG
    ATCAAGACTG ATGTCGGACG AGCTCGAGCA TGGATAAGAT TGTCTCTAGA AAAGAAGGTC
    TTGTCCCAGC ATCTCAAGCA GTTGCTCTCT AACCAGCTGC TCACAAAGAA GCTTTATAAA
    CGTTATGCTT TTCTCCGTTG TGAAGAAGAA AGAGAGCAGT TTCTTTACCA TCTTCTTTCC
    CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTCTTCA CCACTATCAT GATTCCATAT
    AGGTCAGTGA TCATTCCCAT CAAAAAGCTG AGCAACGCGA TCATCACGTC AAACCCTTGG
    ATCTGTGTAT CAGGAGAACT AGGAGACACG GGAGTAATGC AGATTCCCAA AAACCTCCTT
    GAAATGACTT TTGAATGCCA GAATCTGGGG AAGCTGACGA CTGTCCAGAT CGGTCACGAT
    AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA
    GGACATACAT ATAGGTTCCC ATGTGGGCGG TGGCTGGGGA AAGGCATTGA TGATGGAAGT
    CTGGAGAGAA TTCTCATTGG AGAATTGATG ACATCAGCAG CAGATGAGGA TCTAGTAAAG
    CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACTGTGG CCAGGAGACT GAGCATCACA
    TCACTGACAG GAAAAACTAC CAAACCCAAT GCAGGACAGA TCCAAGAAGG AATTGGAGAA
    GCTGTGAACA ACATTGTGAA ACATTTTCAC AAACCTGAAA AAGAGAGAGG AAGCCTCACT
    GTGCTGCTGT GTGGAGAGAG CGGCTTAGTT GCGGCGCTTG AGCAAGTTTT CCACCATGGC
    TTCAAATCAG CTCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTCAT AGAGAGAGCA
    GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGATAATG AAGATGATGT CCTCATTCAG
    AAATCATCCT GCAAAACCTT CTGCCACTAT GTAAATGCTA TTAATACTGC ACCCCGGAAT
    ATTGGGAAGG ATGGCAAATT CCAGATCTTA GTTTGCCTTG GAACACGAGA TCGCCTGCTC
    CCACAGTGGA TTCCTCTGTT AGCCGAGTGT CCTGCCATCA CCCGAATGTA TGAGGAGAGC
    GCCCTCCTGC GAGATCACAT GACGGTCAAC TCCCTTATCC GCATTCTGCA GACCATCCAG
    GACTTCACCA TAGTCTTAGA AGGCTCGCTC ATCAAAGGAG TGGATGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19593
    Clone ID Related Accession (Same CDS sequence) XM_005206831.4 , XM_005206831.3
    Accession Version XM_005206831.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGAAATACAT GCAAGAGGCA CGAAGTTTAG GAAAAAACCT GAGGCAACCC
    AAATTGTCAG ACCTCTCTCC TGCGGTGATT GCACAAACCA ACTGGAAATT TGTAGAAGGC
    TTATTAAAAG AATGTAGAAT GAAGACGAAA CGCATGTTGG TGGAGAAGAT GGGACACGAA
    GCAGTGGAAC TGGGCCATGG AGAAGCCAAC ATAACTGGCT TGGAGGAGAA CACCCTGATT
    GCCAGCCTCT GTGACCTGTT GGAAAGGATA TGGAGCCACG GCTTGCAAGT CAAGCAGGGG
    AAGTCAGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAGAA ACAAGAGCAC
    CTTGCAGAGT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG
    TTGCCAACCC TCAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG
    ATCAAGACTG ATGTCGGACG AGCTCGAGCA TGGATAAGAT TGTCTCTAGA AAAGAAGGTC
    TTGTCCCAGC ATCTCAAGCA GTTGCTCTCT AACCAGCTGC TCACAAAGAA GCTTTATAAA
    CGTTATGCTT TTCTCCGTTG TGAAGAAGAA AGAGAGCAGT TTCTTTACCA TCTTCTTTCC
    CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTCTTCA CCACTATCAT GATTCCATAT
    AGGTCAGTGA TCATTCCCAT CAAAAAGCTG AGCAACGCGA TCATCACGTC AAACCCTTGG
    ATCTGTGTAT CAGGAGAACT AGGAGACACG GGAGTAATGC AGATTCCCAA AAACCTCCTT
    GAAATGACTT TTGAATGCCA GAATCTGGGG AAGCTGACGA CTGTCCAGAT CGGTCACGAT
    AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA
    GGACATACAT ATAGGTTCCC ATGTGGGCGG TGGCTGGGGA AAGGCATTGA TGATGGAAGT
    CTGGAGAGAA TTCTCATTGG AGAATTGATG ACATCAGCAG CAGATGAGGA TCTAGTAAAG
    CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACTGTGG CCAGGAGACT GAGCATCACA
    TCACTGACAG GAAAAACTAC CAAACCCAAT GCAGGACAGA TCCAAGAAGG AATTGGAGAA
    GCTGTGAACA ACATTGTGAA ACATTTTCAC AAACCTGAAA AAGAGAGAGG AAGCCTCACT
    GTGCTGCTGT GTGGAGAGAG CGGCTTAGTT GCGGCGCTTG AGCAAGTTTT CCACCATGGC
    TTCAAATCAG CTCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTCAT AGAGAGAGCA
    GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGATAATG AAGATGATGT CCTCATTCAG
    AAATCATCCT GCAAAACCTT CTGCCACTAT GTAAATGCTA TTAATACTGC ACCCCGGAAT
    ATTGGGAAGG ATGGCAAATT CCAGATCTTA GTTTGCCTTG GAACACGAGA TCGCCTGCTC
    CCACAGTGGA TTCCTCTGTT AGCCGAGTGT CCTGCCATCA CCCGAATGTA TGAGGAGAGC
    GCCCTCCTGC GAGATCACAT GACGGTCAAC TCCCTTATCC GCATTCTGCA GACCATCCAG
    GACTTCACCA TAGTCTTAGA AGGCTCGCTC ATCAAAGGAG TGGATGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206888.1
    CDS286..4176
    Translation

    Target ORF information:

    RefSeq Version XM_005206831.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206831.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGAAATACAT GCAAGAGGCA CGAAGTTTAG GAAAAAACCT GAGGCAACCC
    AAATTGTCAG ACCTCTCTCC TGCGGTGATT GCACAAACCA ACTGGAAATT TGTAGAAGGC
    TTATTAAAAG AATGTAGAAT GAAGACGAAA CGCATGTTGG TGGAGAAGAT GGGACACGAA
    GCAGTGGAAC TGGGCCATGG AGAAGCCAAC ATAACTGGCT TGGAGGAGAA CACCCTGATT
    GCCAGCCTCT GTGACCTGTT GGAAAGGATA TGGAGCCACG GCTTGCAAGT CAAGCAGGGG
    AAGTCAGCTT TGTGGTCACA TTTAATTCAG TTTCAGGACA GAGAAGAGAA ACAAGAGCAC
    CTTGCAGAGT CACCAGTTGC CCTTGGACCA GAAAGAAGAA AATCTGACTC AGGAGTTATG
    TTGCCAACCC TCAGGGTCTC TCTTATCCAA GACATGAGGC ATATTCAAAA CATGAGTGAG
    ATCAAGACTG ATGTCGGACG AGCTCGAGCA TGGATAAGAT TGTCTCTAGA AAAGAAGGTC
    TTGTCCCAGC ATCTCAAGCA GTTGCTCTCT AACCAGCTGC TCACAAAGAA GCTTTATAAA
    CGTTATGCTT TTCTCCGTTG TGAAGAAGAA AGAGAGCAGT TTCTTTACCA TCTTCTTTCC
    CTCAATGCTG TGGACTATTT CTGCTTCACC AGTGTCTTCA CCACTATCAT GATTCCATAT
    AGGTCAGTGA TCATTCCCAT CAAAAAGCTG AGCAACGCGA TCATCACGTC AAACCCTTGG
    ATCTGTGTAT CAGGAGAACT AGGAGACACG GGAGTAATGC AGATTCCCAA AAACCTCCTT
    GAAATGACTT TTGAATGCCA GAATCTGGGG AAGCTGACGA CTGTCCAGAT CGGTCACGAT
    AACTCAGGAC TGTTAGCCAA ATGGCTAGTG GATTGTGTCA TGGTCAGAAA TGAAATCACA
    GGACATACAT ATAGGTTCCC ATGTGGGCGG TGGCTGGGGA AAGGCATTGA TGATGGAAGT
    CTGGAGAGAA TTCTCATTGG AGAATTGATG ACATCAGCAG CAGATGAGGA TCTAGTAAAG
    CAGTGTCGGA CTCCACCCCA GCAGAAATCA CCCACTGTGG CCAGGAGACT GAGCATCACA
    TCACTGACAG GAAAAACTAC CAAACCCAAT GCAGGACAGA TCCAAGAAGG AATTGGAGAA
    GCTGTGAACA ACATTGTGAA ACATTTTCAC AAACCTGAAA AAGAGAGAGG AAGCCTCACT
    GTGCTGCTGT GTGGAGAGAG CGGCTTAGTT GCGGCGCTTG AGCAAGTTTT CCACCATGGC
    TTCAAATCAG CTCGCATCTT TCACAAGAAT GTCTTCATCT GGGACTTCAT AGAGAGAGCA
    GTTGCTTATT TTGAAACCAC TGACCAGATT CTAGATAATG AAGATGATGT CCTCATTCAG
    AAATCATCCT GCAAAACCTT CTGCCACTAT GTAAATGCTA TTAATACTGC ACCCCGGAAT
    ATTGGGAAGG ATGGCAAATT CCAGATCTTA GTTTGCCTTG GAACACGAGA TCGCCTGCTC
    CCACAGTGGA TTCCTCTGTT AGCCGAGTGT CCTGCCATCA CCCGAATGTA TGAGGAGAGC
    GCCCTCCTGC GAGATCACAT GACGGTCAAC TCCCTTATCC GCATTCTGCA GACCATCCAG
    GACTTCACCA TAGTCTTAGA AGGCTCGCTC ATCAAAGGAG TGGATGTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90783
    Clone ID Related Accession (Same CDS sequence) XM_015471173.1
    Accession Version XM_015471173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4089bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGC CTTCCGGGGA ACTTGTGGTC
    TGTCAGAACT CCATGGCATT CTGGGAGTGG GATCAGGGTC CACCTCCTCC TGCACGACTT
    CCTAAAAAAT CCTTCTCTGA GTGCTGCCTG AAATACATGC AAGAGGCACG AAGTTTAGGA
    AAAAACCTGA GGCAACCCAA ATTGTCAGAC CTCTCTCCTG CGGTGATTGC ACAAACCAAC
    TGGAAATTTG TAGAAGGCTT ATTAAAAGAA TGTAGAATGA AGACGAAACG CATGTTGGTG
    GAGAAGATGG GACACGAAGC AGTGGAACTG GGCCATGGAG AAGCCAACAT AACTGGCTTG
    GAGGAGAACA CCCTGATTGC CAGCCTCTGT GACCTGTTGG AAAGGATATG GAGCCACGGC
    TTGCAAGTCA AGCAGGGGAA GTCAGCTTTG TGGTCACATT TAATTCAGTT TCAGGACAGA
    GAAGAGAAAC AAGAGCACCT TGCAGAGTCA CCAGTTGCCC TTGGACCAGA AAGAAGAAAA
    TCTGACTCAG GAGTTATGTT GCCAACCCTC AGGGTCTCTC TTATCCAAGA CATGAGGCAT
    ATTCAAAACA TGAGTGAGAT CAAGACTGAT GTCGGACGAG CTCGAGCATG GATAAGATTG
    TCTCTAGAAA AGAAGGTCTT GTCCCAGCAT CTCAAGCAGT TGCTCTCTAA CCAGCTGCTC
    ACAAAGAAGC TTTATAAACG TTATGCTTTT CTCCGTTGTG AAGAAGAAAG AGAGCAGTTT
    CTTTACCATC TTCTTTCCCT CAATGCTGTG GACTATTTCT GCTTCACCAG TGTCTTCACC
    ACTATCATGA TTCCATATAG GTCAGTGATC ATTCCCATCA AAAAGCTGAG CAACGCGATC
    ATCACGTCAA ACCCTTGGAT CTGTGTATCA GGAGAACTAG GAGACACGGG AGTAATGCAG
    ATTCCCAAAA ACCTCCTTGA AATGACTTTT GAATGCCAGA ATCTGGGGAA GCTGACGACT
    GTCCAGATCG GTCACGATAA CTCAGGACTG TTAGCCAAAT GGCTAGTGGA TTGTGTCATG
    GTCAGAAATG AAATCACAGG ACATACATAT AGGTTCCCAT GTGGGCGGTG GCTGGGGAAA
    GGCATTGATG ATGGAAGTCT GGAGAGAATT CTCATTGGAG AATTGATGAC ATCAGCAGCA
    GATGAGGATC TAGTAAAGCA GTGTCGGACT CCACCCCAGC AGAAATCACC CACTGTGGCC
    AGGAGACTGA GCATCACATC ACTGACAGGA AAAACTACCA AACCCAATGC AGGACAGATC
    CAAGAAGGAA TTGGAGAAGC TGTGAACAAC ATTGTGAAAC ATTTTCACAA ACCTGAAAAA
    GAGAGAGGAA GCCTCACTGT GCTGCTGTGT GGAGAGAGCG GCTTAGTTGC GGCGCTTGAG
    CAAGTTTTCC ACCATGGCTT CAAATCAGCT CGCATCTTTC ACAAGAATGT CTTCATCTGG
    GACTTCATAG AGAGAGCAGT TGCTTATTTT GAAACCACTG ACCAGATTCT AGATAATGAA
    GATGATGTCC TCATTCAGAA ATCATCCTGC AAAACCTTCT GCCACTATGT AAATGCTATT
    AATACTGCAC CCCGGAATAT TGGGAAGGAT GGCAAATTCC AGATCTTAGT TTGCCTTGGA
    ACACGAGATC GCCTGCTCCC ACAGTGGATT CCTCTGTTAG CCGAGTGTCC TGCCATCACC
    CGAATGTATG AGGAGAGCGC CCTCCTGCGA GATCACATGA CGGTCAACTC CCTTATCCGC
    ATTCTGCAGA CCATCCAGGA CTTCACCATA GTCTTAGAAG GCTCGCTCAT CAAAGGAGTG
    GATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326659.1
    CDS290..4378
    Translation

    Target ORF information:

    RefSeq Version XM_015471173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGC CTTCCGGGGA ACTTGTGGTC
    TGTCAGAACT CCATGGCATT CTGGGAGTGG GATCAGGGTC CACCTCCTCC TGCACGACTT
    CCTAAAAAAT CCTTCTCTGA GTGCTGCCTG AAATACATGC AAGAGGCACG AAGTTTAGGA
    AAAAACCTGA GGCAACCCAA ATTGTCAGAC CTCTCTCCTG CGGTGATTGC ACAAACCAAC
    TGGAAATTTG TAGAAGGCTT ATTAAAAGAA TGTAGAATGA AGACGAAACG CATGTTGGTG
    GAGAAGATGG GACACGAAGC AGTGGAACTG GGCCATGGAG AAGCCAACAT AACTGGCTTG
    GAGGAGAACA CCCTGATTGC CAGCCTCTGT GACCTGTTGG AAAGGATATG GAGCCACGGC
    TTGCAAGTCA AGCAGGGGAA GTCAGCTTTG TGGTCACATT TAATTCAGTT TCAGGACAGA
    GAAGAGAAAC AAGAGCACCT TGCAGAGTCA CCAGTTGCCC TTGGACCAGA AAGAAGAAAA
    TCTGACTCAG GAGTTATGTT GCCAACCCTC AGGGTCTCTC TTATCCAAGA CATGAGGCAT
    ATTCAAAACA TGAGTGAGAT CAAGACTGAT GTCGGACGAG CTCGAGCATG GATAAGATTG
    TCTCTAGAAA AGAAGGTCTT GTCCCAGCAT CTCAAGCAGT TGCTCTCTAA CCAGCTGCTC
    ACAAAGAAGC TTTATAAACG TTATGCTTTT CTCCGTTGTG AAGAAGAAAG AGAGCAGTTT
    CTTTACCATC TTCTTTCCCT CAATGCTGTG GACTATTTCT GCTTCACCAG TGTCTTCACC
    ACTATCATGA TTCCATATAG GTCAGTGATC ATTCCCATCA AAAAGCTGAG CAACGCGATC
    ATCACGTCAA ACCCTTGGAT CTGTGTATCA GGAGAACTAG GAGACACGGG AGTAATGCAG
    ATTCCCAAAA ACCTCCTTGA AATGACTTTT GAATGCCAGA ATCTGGGGAA GCTGACGACT
    GTCCAGATCG GTCACGATAA CTCAGGACTG TTAGCCAAAT GGCTAGTGGA TTGTGTCATG
    GTCAGAAATG AAATCACAGG ACATACATAT AGGTTCCCAT GTGGGCGGTG GCTGGGGAAA
    GGCATTGATG ATGGAAGTCT GGAGAGAATT CTCATTGGAG AATTGATGAC ATCAGCAGCA
    GATGAGGATC TAGTAAAGCA GTGTCGGACT CCACCCCAGC AGAAATCACC CACTGTGGCC
    AGGAGACTGA GCATCACATC ACTGACAGGA AAAACTACCA AACCCAATGC AGGACAGATC
    CAAGAAGGAA TTGGAGAAGC TGTGAACAAC ATTGTGAAAC ATTTTCACAA ACCTGAAAAA
    GAGAGAGGAA GCCTCACTGT GCTGCTGTGT GGAGAGAGCG GCTTAGTTGC GGCGCTTGAG
    CAAGTTTTCC ACCATGGCTT CAAATCAGCT CGCATCTTTC ACAAGAATGT CTTCATCTGG
    GACTTCATAG AGAGAGCAGT TGCTTATTTT GAAACCACTG ACCAGATTCT AGATAATGAA
    GATGATGTCC TCATTCAGAA ATCATCCTGC AAAACCTTCT GCCACTATGT AAATGCTATT
    AATACTGCAC CCCGGAATAT TGGGAAGGAT GGCAAATTCC AGATCTTAGT TTGCCTTGGA
    ACACGAGATC GCCTGCTCCC ACAGTGGATT CCTCTGTTAG CCGAGTGTCC TGCCATCACC
    CGAATGTATG AGGAGAGCGC CCTCCTGCGA GATCACATGA CGGTCAACTC CCTTATCCGC
    ATTCTGCAGA CCATCCAGGA CTTCACCATA GTCTTAGAAG GCTCGCTCAT CAAAGGAGTG
    GATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90784
    Clone ID Related Accession (Same CDS sequence) XM_015471174.1
    Accession Version XM_015471174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4023bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GGTGAACGCT TTAGGCAGAG GCTATCTGCT GCTGATATTG TGGCACCAGA ACCTCATGAT
    TTCTTTGCAG AAAGACCACA AGTTGCTACC CAGTCAATTG AGCAGCGACT GATGAAAATG
    GATCACACCG CAATCCACCC ACATCTACTT GATATGAAAA TTGGTCAAGG CAAATACGAG
    CAAGGATTTT TTCCAAAGTT ACAGTCTGAT GTCTTGGCAA CAGGACCAAC TAATAACAAT
    CGCTGGGTGA GTCGGAGTGC CACTGCACAG CGCAGGAAAG ACCGCCTTCG CCAGCATTCC
    GACCACGTTG GGCTGGACAA CGACTTGAGG GAGCCTTCCG GGGAACTTGT GGTCTGTCAG
    AACTCCATGG CATTCTGGGA GTGGGATCAG GGTCCACCTC CTCCTGCACG ACTTCCTAAA
    AAATCCTTCT CTGAGTGCTG CCTGAAATAC ATGCAAGAGG CACGAAGTTT AGGAAAAAAC
    CTGAGGCAAC CCAAATTGTC AGACCTCTCT CCTGCGGTGA TTGCACAAAC CAACTGGAAA
    TTTGTAGAAG GCTTATTAAA AGAATGTAGA ATGAAGACGA AACGCATGTT GGTGGAGAAG
    ATGGGACACG AAGCAGTGGA ACTGGGCCAT GGAGAAGCCA ACATAACTGG CTTGGAGGAG
    AACACCCTGA TTGCCAGCCT CTGTGACCTG TTGGAAAGGA TATGGAGCCA CGGCTTGCAA
    GTCAAGCAGG GGAAGTCAGC TTTGTGGTCA CATTTAATTC AGTTTCAGGA CAGAGAAGAG
    AAACAAGAGC ACCTTGCAGA GTCACCAGTT GCCCTTGGAC CAGAAAGAAG AAAATCTGAC
    TCAGGAGTTA TGTTGCCAAC CCTCAGGGTC TCTCTTATCC AAGACATGAG GCATATTCAA
    AACATGAGTG AGATCAAGAC TGATGTCGGA CGAGCTCGAG CATGGATAAG ATTGTCTCTA
    GAAAAGAAGG TCTTGTCCCA GCATCTCAAG CAGTTGCTCT CTAACCAGCT GCTCACAAAG
    AAGCTTTATA AACGTTATGC TTTTCTCCGT TGTGAAGAAG AAAGAGAGCA GTTTCTTTAC
    CATCTTCTTT CCCTCAATGC TGTGGACTAT TTCTGCTTCA CCAGTGTCTT CACCACTATC
    ATGATTCCAT ATAGGTCAGT GATCATTCCC ATCAAAAAGC TGAGCAACGC GATCATCACG
    TCAAACCCTT GGATCTGTGT ATCAGGAGAA CTAGGAGACA CGGGAGTAAT GCAGATTCCC
    AAAAACCTCC TTGAAATGAC TTTTGAATGC CAGAATCTGG GGAAGCTGAC GACTGTCCAG
    ATCGGTCACG ATAACTCAGG ACTGTTAGCC AAATGGCTAG TGGATTGTGT CATGGTCAGA
    AATGAAATCA CAGGACATAC ATATAGGTTC CCATGTGGGC GGTGGCTGGG GAAAGGCATT
    GATGATGGAA GTCTGGAGAG AATTCTCATT GGAGAATTGA TGACATCAGC AGCAGATGAG
    GATCTAGTAA AGCAGTGTCG GACTCCACCC CAGCAGAAAT CACCCACTGT GGCCAGGAGA
    CTGAGCATCA CATCACTGAC AGGAAAAACT ACCAAACCCA ATGCAGGACA GATCCAAGAA
    GGAATTGGAG AAGCTGTGAA CAACATTGTG AAACATTTTC ACAAACCTGA AAAAGAGAGA
    GGAAGCCTCA CTGTGCTGCT GTGTGGAGAG AGCGGCTTAG TTGCGGCGCT TGAGCAAGTT
    TTCCACCATG GCTTCAAATC AGCTCGCATC TTTCACAAGA ATGTCTTCAT CTGGGACTTC
    ATAGAGAGAG CAGTTGCTTA TTTTGAAACC ACTGACCAGA TTCTAGATAA TGAAGATGAT
    GTCCTCATTC AGAAATCATC CTGCAAAACC TTCTGCCACT ATGTAAATGC TATTAATACT
    GCACCCCGGA ATATTGGGAA GGATGGCAAA TTCCAGATCT TAGTTTGCCT TGGAACACGA
    GATCGCCTGC TCCCACAGTG GATTCCTCTG TTAGCCGAGT GTCCTGCCAT CACCCGAATG
    TATGAGGAGA GCGCCCTCCT GCGAGATCAC ATGACGGTCA ACTCCCTTAT CCGCATTCTG
    CAGACCATCC AGGACTTCAC CATAGTCTTA GAAGGCTCGC TCATCAAAGG AGTGGATGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326660.1
    CDS289..4311
    Translation

    Target ORF information:

    RefSeq Version XM_015471174.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGGTG AAAATTTTGA CCAGAGTCCT TTGAGAAGAA CATTCAAATC CAAAGTTCTG
    GCCCACTATC CTCAGAATAT AGAATGGAAC CCTTTCGATC AAGATGCAGT AAACATGTTG
    TGCATGCCTA AAGGGCTGTC TTTCAGAACA CAAACTGACA ACAAAGACCC TCAGTTCCAC
    TCATTTATAA TTACCCGGGA AGATGGATCT CGTACCTACG GTTTTGTTCT CACTTTTTAT
    GAAGAAGTTA CAAGTAAGCA AATCTGCACA GCAATGCAGA CACTCTACCA GATGCACAAT
    GCAGAGCATT ACAGCAGCGT GTACGCTTCA TCCTCCTGCA GCATGGATTC ACTGGCGAGC
    AGTATTGACG AGGGAGATAC AACTTCCCTT TTGAAACTCC AGCGATACAA CTCCTATGAC
    ATCAGCAGAG ACACCCTGTA TGTTTCAAAA AGTATATGTT TGATCACACC ACTGCCTTTC
    ATGCAGGCCT GCAAGAAATT CCTCATCCAA CTTTACAAGG CAGTTACCTC ACAGCAGCCA
    CCACCTTTGC CTCTTGAGAG CTACATCCAT AATATTCTCT ATGAGGTGCC CCTTCCACCT
    CCAGGAAGAT CACTGAAATT TTATGGTGTT TATGAACCTG TCATCTGCCA GAGGCCGGGG
    CTCAATGAAC TCCCCCTCTC CGATTACCCT CTCCGAGAGG TCTTTGAGCT CCTGGGCCTA
    GAGAACCTGG TGCAGGTATT TACCTGTGTT CTTCTGGAGA TGCAGATCCT TCTCTATTCA
    CAAGATTATC AGCGCCTGAT GACTGTGGCT GAAGGCATCA CCACACTTCT GTTCCCATTT
    CAGTGGCAGC ATGTTTATGT GCCCATCCTC CCTGCTTCTC TGCTCCACTT TCTTGATGCT
    CCTGTCCCTT ATCTGATGGG CCTTCAGTCA AAAGAAGGAA CTGACCGTTC TAAGCTAGAA
    CTTCCTCAAG AGGCTAACTT GTGTTTTGTA GATATTGACA ACCATTTTAT TGAGTTGCCT
    GAAGAATTTC CACAGTTCCC CAATAAAGTG GATTTTATCC AGGAACTCTC TGAAGTTCTT
    CTTCAGTTTG GGATCCCTCC TGAGGGCAGT CTACATTGCA GTGAGAGTGC CACCAAGCTC
    AAGAATCTCG TTCTGAAAGA CTTGGTCAAT GACAAGAAGA ATGGCAATGT CTCTGCAAAT
    AGCATCAGCA TGTATGAGTT ACTGAAGGGC AACGAAACCA TAGCTCGTCT CCAGGCTCTG
    GCCAAGCGGA CTGGTGTGAC TATGGAAAAA ATGGACCTCT CTGCCTCTCT GAGTGAAAAA
    GAAAAAGACT TAAAACTGCA GTGTGAAGAC GCAGAACTAA GGGACTACCA GCTCAATGTT
    CAGCTCCGAG AGGTCTTTGC CAACCGCTTT ACACAGATGT TTGCAGATTA TGAAGCGTTT
    GTGATTCAGA CTGCCCAGGA CCTGGAATCC TGGCTGACCA ACCGGGAACA GATGCAAAAC
    TTTGATAAAG CTTCCTTCCT GTCTGACCAG CCAGAGCCGT ACCTGCCATT TCTTTCACGC
    TTCATTGAAA CACAGATGTT TGCCACCTTT ATTGATAACA AAATTATGTC TCAATGGGAG
    GAGAAAGATC CTTTGCTTCG AGTCTTTGAC TCTCGGATTG AGAAGATAAG ACTGTATAAT
    GTAAGGGCAC CCACCTTGAG GACATCTATT TACCAGAAAT GCAGCACTTT AAAAGAAGCA
    GGTGAACGCT TTAGGCAGAG GCTATCTGCT GCTGATATTG TGGCACCAGA ACCTCATGAT
    TTCTTTGCAG AAAGACCACA AGTTGCTACC CAGTCAATTG AGCAGCGACT GATGAAAATG
    GATCACACCG CAATCCACCC ACATCTACTT GATATGAAAA TTGGTCAAGG CAAATACGAG
    CAAGGATTTT TTCCAAAGTT ACAGTCTGAT GTCTTGGCAA CAGGACCAAC TAATAACAAT
    CGCTGGGTGA GTCGGAGTGC CACTGCACAG CGCAGGAAAG ACCGCCTTCG CCAGCATTCC
    GACCACGTTG GGCTGGACAA CGACTTGAGG GAGCCTTCCG GGGAACTTGT GGTCTGTCAG
    AACTCCATGG CATTCTGGGA GTGGGATCAG GGTCCACCTC CTCCTGCACG ACTTCCTAAA
    AAATCCTTCT CTGAGTGCTG CCTGAAATAC ATGCAAGAGG CACGAAGTTT AGGAAAAAAC
    CTGAGGCAAC CCAAATTGTC AGACCTCTCT CCTGCGGTGA TTGCACAAAC CAACTGGAAA
    TTTGTAGAAG GCTTATTAAA AGAATGTAGA ATGAAGACGA AACGCATGTT GGTGGAGAAG
    ATGGGACACG AAGCAGTGGA ACTGGGCCAT GGAGAAGCCA ACATAACTGG CTTGGAGGAG
    AACACCCTGA TTGCCAGCCT CTGTGACCTG TTGGAAAGGA TATGGAGCCA CGGCTTGCAA
    GTCAAGCAGG GGAAGTCAGC TTTGTGGTCA CATTTAATTC AGTTTCAGGA CAGAGAAGAG
    AAACAAGAGC ACCTTGCAGA GTCACCAGTT GCCCTTGGAC CAGAAAGAAG AAAATCTGAC
    TCAGGAGTTA TGTTGCCAAC CCTCAGGGTC TCTCTTATCC AAGACATGAG GCATATTCAA
    AACATGAGTG AGATCAAGAC TGATGTCGGA CGAGCTCGAG CATGGATAAG ATTGTCTCTA
    GAAAAGAAGG TCTTGTCCCA GCATCTCAAG CAGTTGCTCT CTAACCAGCT GCTCACAAAG
    AAGCTTTATA AACGTTATGC TTTTCTCCGT TGTGAAGAAG AAAGAGAGCA GTTTCTTTAC
    CATCTTCTTT CCCTCAATGC TGTGGACTAT TTCTGCTTCA CCAGTGTCTT CACCACTATC
    ATGATTCCAT ATAGGTCAGT GATCATTCCC ATCAAAAAGC TGAGCAACGC GATCATCACG
    TCAAACCCTT GGATCTGTGT ATCAGGAGAA CTAGGAGACA CGGGAGTAAT GCAGATTCCC
    AAAAACCTCC TTGAAATGAC TTTTGAATGC CAGAATCTGG GGAAGCTGAC GACTGTCCAG
    ATCGGTCACG ATAACTCAGG ACTGTTAGCC AAATGGCTAG TGGATTGTGT CATGGTCAGA
    AATGAAATCA CAGGACATAC ATATAGGTTC CCATGTGGGC GGTGGCTGGG GAAAGGCATT
    GATGATGGAA GTCTGGAGAG AATTCTCATT GGAGAATTGA TGACATCAGC AGCAGATGAG
    GATCTAGTAA AGCAGTGTCG GACTCCACCC CAGCAGAAAT CACCCACTGT GGCCAGGAGA
    CTGAGCATCA CATCACTGAC AGGAAAAACT ACCAAACCCA ATGCAGGACA GATCCAAGAA
    GGAATTGGAG AAGCTGTGAA CAACATTGTG AAACATTTTC ACAAACCTGA AAAAGAGAGA
    GGAAGCCTCA CTGTGCTGCT GTGTGGAGAG AGCGGCTTAG TTGCGGCGCT TGAGCAAGTT
    TTCCACCATG GCTTCAAATC AGCTCGCATC TTTCACAAGA ATGTCTTCAT CTGGGACTTC
    ATAGAGAGAG CAGTTGCTTA TTTTGAAACC ACTGACCAGA TTCTAGATAA TGAAGATGAT
    GTCCTCATTC AGAAATCATC CTGCAAAACC TTCTGCCACT ATGTAAATGC TATTAATACT
    GCACCCCGGA ATATTGGGAA GGATGGCAAA TTCCAGATCT TAGTTTGCCT TGGAACACGA
    GATCGCCTGC TCCCACAGTG GATTCCTCTG TTAGCCGAGT GTCCTGCCAT CACCCGAATG
    TATGAGGAGA GCGCCCTCCT GCGAGATCAC ATGACGGTCA ACTCCCTTAT CCGCATTCTG
    CAGACCATCC AGGACTTCAC CATAGTCTTA GAAGGCTCGC TCATCAAAGG AGTGGATGTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90785
    Clone ID Related Accession (Same CDS sequence) XM_015471175.1
    Accession Version XM_015471175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4002bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGCCTTCCGG GGAACTTGTG GTCTGTCAGA ACTCCATGGC ATTCTGGGAG
    TGGGATCAGG GTCCACCTCC TCCTGCACGA CTTCCTAAAA AATCCTTCTC TGAGTGCTGC
    CTGAAATACA TGCAAGAGGC ACGAAGTTTA GGAAAAAACC TGAGGCAACC CAAATTGTCA
    GACCTCTCTC CTGCGGTGAT TGCACAAACC AACTGGAAAT TTGTAGAAGG CTTATTAAAA
    GAATGTAGAA TGAAGACGAA ACGCATGTTG GTGGAGAAGA TGGGACACGA AGCAGTGGAA
    CTGGGCCATG GAGAAGCCAA CATAACTGGC TTGGAGGAGA ACACCCTGAT TGCCAGCCTC
    TGTGACCTGT TGGAAAGGAT ATGGAGCCAC GGCTTGCAAG TCAAGCAGGG GAAGTCAGCT
    TTGTGGTCAC ATTTAATTCA GTTTCAGGAC AGAGAAGAGA AACAAGAGCA CCTTGCAGAG
    TCACCAGTTG CCCTTGGACC AGAAAGAAGA AAATCTGACT CAGGAGTTAT GTTGCCAACC
    CTCAGGGTCT CTCTTATCCA AGACATGAGG CATATTCAAA ACATGAGTGA GATCAAGACT
    GATGTCGGAC GAGCTCGAGC ATGGATAAGA TTGTCTCTAG AAAAGAAGGT CTTGTCCCAG
    CATCTCAAGC AGTTGCTCTC TAACCAGCTG CTCACAAAGA AGCTTTATAA ACGTTATGCT
    TTTCTCCGTT GTGAAGAAGA AAGAGAGCAG TTTCTTTACC ATCTTCTTTC CCTCAATGCT
    GTGGACTATT TCTGCTTCAC CAGTGTCTTC ACCACTATCA TGATTCCATA TAGGTCAGTG
    ATCATTCCCA TCAAAAAGCT GAGCAACGCG ATCATCACGT CAAACCCTTG GATCTGTGTA
    TCAGGAGAAC TAGGAGACAC GGGAGTAATG CAGATTCCCA AAAACCTCCT TGAAATGACT
    TTTGAATGCC AGAATCTGGG GAAGCTGACG ACTGTCCAGA TCGGTCACGA TAACTCAGGA
    CTGTTAGCCA AATGGCTAGT GGATTGTGTC ATGGTCAGAA ATGAAATCAC AGGACATACA
    TATAGGTTCC CATGTGGGCG GTGGCTGGGG AAAGGCATTG ATGATGGAAG TCTGGAGAGA
    ATTCTCATTG GAGAATTGAT GACATCAGCA GCAGATGAGG ATCTAGTAAA GCAGTGTCGG
    ACTCCACCCC AGCAGAAATC ACCCACTGTG GCCAGGAGAC TGAGCATCAC ATCACTGACA
    GGAAAAACTA CCAAACCCAA TGCAGGACAG ATCCAAGAAG GAATTGGAGA AGCTGTGAAC
    AACATTGTGA AACATTTTCA CAAACCTGAA AAAGAGAGAG GAAGCCTCAC TGTGCTGCTG
    TGTGGAGAGA GCGGCTTAGT TGCGGCGCTT GAGCAAGTTT TCCACCATGG CTTCAAATCA
    GCTCGCATCT TTCACAAGAA TGTCTTCATC TGGGACTTCA TAGAGAGAGC AGTTGCTTAT
    TTTGAAACCA CTGACCAGAT TCTAGATAAT GAAGATGATG TCCTCATTCA GAAATCATCC
    TGCAAAACCT TCTGCCACTA TGTAAATGCT ATTAATACTG CACCCCGGAA TATTGGGAAG
    GATGGCAAAT TCCAGATCTT AGTTTGCCTT GGAACACGAG ATCGCCTGCT CCCACAGTGG
    ATTCCTCTGT TAGCCGAGTG TCCTGCCATC ACCCGAATGT ATGAGGAGAG CGCCCTCCTG
    CGAGATCACA TGACGGTCAA CTCCCTTATC CGCATTCTGC AGACCATCCA GGACTTCACC
    ATAGTCTTAG AAGGCTCGCT CATCAAAGGA GTGGATGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326661.1
    CDS288..4289
    Translation

    Target ORF information:

    RefSeq Version XM_015471175.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGCCC AGTCAATTGA GCAGCGACTG ATGAAAATGG ATCACACCGC AATCCACCCA
    CATCTACTTG ATATGAAAAT TGGTCAAGGC AAATACGAGC AAGGATTTTT TCCAAAGTTA
    CAGTCTGATG TCTTGGCAAC AGGACCAACT AATAACAATC GCTGGGTGAG TCGGAGTGCC
    ACTGCACAGC GCAGGAAAGA CCGCCTTCGC CAGCATTCCG ACCACGTTGG GCTGGACAAC
    GACTTGAGGG AGCCTTCCGG GGAACTTGTG GTCTGTCAGA ACTCCATGGC ATTCTGGGAG
    TGGGATCAGG GTCCACCTCC TCCTGCACGA CTTCCTAAAA AATCCTTCTC TGAGTGCTGC
    CTGAAATACA TGCAAGAGGC ACGAAGTTTA GGAAAAAACC TGAGGCAACC CAAATTGTCA
    GACCTCTCTC CTGCGGTGAT TGCACAAACC AACTGGAAAT TTGTAGAAGG CTTATTAAAA
    GAATGTAGAA TGAAGACGAA ACGCATGTTG GTGGAGAAGA TGGGACACGA AGCAGTGGAA
    CTGGGCCATG GAGAAGCCAA CATAACTGGC TTGGAGGAGA ACACCCTGAT TGCCAGCCTC
    TGTGACCTGT TGGAAAGGAT ATGGAGCCAC GGCTTGCAAG TCAAGCAGGG GAAGTCAGCT
    TTGTGGTCAC ATTTAATTCA GTTTCAGGAC AGAGAAGAGA AACAAGAGCA CCTTGCAGAG
    TCACCAGTTG CCCTTGGACC AGAAAGAAGA AAATCTGACT CAGGAGTTAT GTTGCCAACC
    CTCAGGGTCT CTCTTATCCA AGACATGAGG CATATTCAAA ACATGAGTGA GATCAAGACT
    GATGTCGGAC GAGCTCGAGC ATGGATAAGA TTGTCTCTAG AAAAGAAGGT CTTGTCCCAG
    CATCTCAAGC AGTTGCTCTC TAACCAGCTG CTCACAAAGA AGCTTTATAA ACGTTATGCT
    TTTCTCCGTT GTGAAGAAGA AAGAGAGCAG TTTCTTTACC ATCTTCTTTC CCTCAATGCT
    GTGGACTATT TCTGCTTCAC CAGTGTCTTC ACCACTATCA TGATTCCATA TAGGTCAGTG
    ATCATTCCCA TCAAAAAGCT GAGCAACGCG ATCATCACGT CAAACCCTTG GATCTGTGTA
    TCAGGAGAAC TAGGAGACAC GGGAGTAATG CAGATTCCCA AAAACCTCCT TGAAATGACT
    TTTGAATGCC AGAATCTGGG GAAGCTGACG ACTGTCCAGA TCGGTCACGA TAACTCAGGA
    CTGTTAGCCA AATGGCTAGT GGATTGTGTC ATGGTCAGAA ATGAAATCAC AGGACATACA
    TATAGGTTCC CATGTGGGCG GTGGCTGGGG AAAGGCATTG ATGATGGAAG TCTGGAGAGA
    ATTCTCATTG GAGAATTGAT GACATCAGCA GCAGATGAGG ATCTAGTAAA GCAGTGTCGG
    ACTCCACCCC AGCAGAAATC ACCCACTGTG GCCAGGAGAC TGAGCATCAC ATCACTGACA
    GGAAAAACTA CCAAACCCAA TGCAGGACAG ATCCAAGAAG GAATTGGAGA AGCTGTGAAC
    AACATTGTGA AACATTTTCA CAAACCTGAA AAAGAGAGAG GAAGCCTCAC TGTGCTGCTG
    TGTGGAGAGA GCGGCTTAGT TGCGGCGCTT GAGCAAGTTT TCCACCATGG CTTCAAATCA
    GCTCGCATCT TTCACAAGAA TGTCTTCATC TGGGACTTCA TAGAGAGAGC AGTTGCTTAT
    TTTGAAACCA CTGACCAGAT TCTAGATAAT GAAGATGATG TCCTCATTCA GAAATCATCC
    TGCAAAACCT TCTGCCACTA TGTAAATGCT ATTAATACTG CACCCCGGAA TATTGGGAAG
    GATGGCAAAT TCCAGATCTT AGTTTGCCTT GGAACACGAG ATCGCCTGCT CCCACAGTGG
    ATTCCTCTGT TAGCCGAGTG TCCTGCCATC ACCCGAATGT ATGAGGAGAG CGCCCTCCTG
    CGAGATCACA TGACGGTCAA CTCCCTTATC CGCATTCTGC AGACCATCCA GGACTTCACC
    ATAGTCTTAG AAGGCTCGCT CATCAAAGGA GTGGATGTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90786
    Clone ID Related Accession (Same CDS sequence) XM_015471176.1 , XM_015471176.2
    Accession Version XM_015471176.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103930.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGA AATACATGCA AGAGGCACGA
    AGTTTAGGAA AAAACCTGAG GCAACCCAAA TTGTCAGACC TCTCTCCTGC GGTGATTGCA
    CAAACCAACT GGAAATTTGT AGAAGGCTTA TTAAAAGAAT GTAGAATGAA GACGAAACGC
    ATGTTGGTGG AGAAGATGGG ACACGAAGCA GTGGAACTGG GCCATGGAGA AGCCAACATA
    ACTGGCTTGG AGGAGAACAC CCTGATTGCC AGCCTCTGTG ACCTGTTGGA AAGGATATGG
    AGCCACGGCT TGCAAGTCAA GCAGGGGAAG TCAGCTTTGT GGTCACATTT AATTCAGTTT
    CAGGACAGAG AAGAGAAACA AGAGCACCTT GCAGAGTCAC CAGTTGCCCT TGGACCAGAA
    AGAAGAAAAT CTGACTCAGG AGTTATGTTG CCAACCCTCA GGGTCTCTCT TATCCAAGAC
    ATGAGGCATA TTCAAAACAT GAGTGAGATC AAGACTGATG TCGGACGAGC TCGAGCATGG
    ATAAGATTGT CTCTAGAAAA GAAGGTCTTG TCCCAGCATC TCAAGCAGTT GCTCTCTAAC
    CAGCTGCTCA CAAAGAAGCT TTATAAACGT TATGCTTTTC TCCGTTGTGA AGAAGAAAGA
    GAGCAGTTTC TTTACCATCT TCTTTCCCTC AATGCTGTGG ACTATTTCTG CTTCACCAGT
    GTCTTCACCA CTATCATGAT TCCATATAGG TCAGTGATCA TTCCCATCAA AAAGCTGAGC
    AACGCGATCA TCACGTCAAA CCCTTGGATC TGTGTATCAG GAGAACTAGG AGACACGGGA
    GTAATGCAGA TTCCCAAAAA CCTCCTTGAA ATGACTTTTG AATGCCAGAA TCTGGGGAAG
    CTGACGACTG TCCAGATCGG TCACGATAAC TCAGGACTGT TAGCCAAATG GCTAGTGGAT
    TGTGTCATGG TCAGAAATGA AATCACAGGA CATACATATA GGTTCCCATG TGGGCGGTGG
    CTGGGGAAAG GCATTGATGA TGGAAGTCTG GAGAGAATTC TCATTGGAGA ATTGATGACA
    TCAGCAGCAG ATGAGGATCT AGTAAAGCAG TGTCGGACTC CACCCCAGCA GAAATCACCC
    ACTGTGGCCA GGAGACTGAG CATCACATCA CTGACAGGAA AAACTACCAA ACCCAATGCA
    GGACAGATCC AAGAAGGAAT TGGAGAAGCT GTGAACAACA TTGTGAAACA TTTTCACAAA
    CCTGAAAAAG AGAGAGGAAG CCTCACTGTG CTGCTGTGTG GAGAGAGCGG CTTAGTTGCG
    GCGCTTGAGC AAGTTTTCCA CCATGGCTTC AAATCAGCTC GCATCTTTCA CAAGAATGTC
    TTCATCTGGG ACTTCATAGA GAGAGCAGTT GCTTATTTTG AAACCACTGA CCAGATTCTA
    GATAATGAAG ATGATGTCCT CATTCAGAAA TCATCCTGCA AAACCTTCTG CCACTATGTA
    AATGCTATTA ATACTGCACC CCGGAATATT GGGAAGGATG GCAAATTCCA GATCTTAGTT
    TGCCTTGGAA CACGAGATCG CCTGCTCCCA CAGTGGATTC CTCTGTTAGC CGAGTGTCCT
    GCCATCACCC GAATGTATGA GGAGAGCGCC CTCCTGCGAG ATCACATGAC GGTCAACTCC
    CTTATCCGCA TTCTGCAGAC CATCCAGGAC TTCACCATAG TCTTAGAAGG CTCGCTCATC
    AAAGGAGTGG ATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326662.1
    CDS288..4265
    Translation

    Target ORF information:

    RefSeq Version XM_015471176.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN/MADD domain containing 5B (DENND5B), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGA AATACATGCA AGAGGCACGA
    AGTTTAGGAA AAAACCTGAG GCAACCCAAA TTGTCAGACC TCTCTCCTGC GGTGATTGCA
    CAAACCAACT GGAAATTTGT AGAAGGCTTA TTAAAAGAAT GTAGAATGAA GACGAAACGC
    ATGTTGGTGG AGAAGATGGG ACACGAAGCA GTGGAACTGG GCCATGGAGA AGCCAACATA
    ACTGGCTTGG AGGAGAACAC CCTGATTGCC AGCCTCTGTG ACCTGTTGGA AAGGATATGG
    AGCCACGGCT TGCAAGTCAA GCAGGGGAAG TCAGCTTTGT GGTCACATTT AATTCAGTTT
    CAGGACAGAG AAGAGAAACA AGAGCACCTT GCAGAGTCAC CAGTTGCCCT TGGACCAGAA
    AGAAGAAAAT CTGACTCAGG AGTTATGTTG CCAACCCTCA GGGTCTCTCT TATCCAAGAC
    ATGAGGCATA TTCAAAACAT GAGTGAGATC AAGACTGATG TCGGACGAGC TCGAGCATGG
    ATAAGATTGT CTCTAGAAAA GAAGGTCTTG TCCCAGCATC TCAAGCAGTT GCTCTCTAAC
    CAGCTGCTCA CAAAGAAGCT TTATAAACGT TATGCTTTTC TCCGTTGTGA AGAAGAAAGA
    GAGCAGTTTC TTTACCATCT TCTTTCCCTC AATGCTGTGG ACTATTTCTG CTTCACCAGT
    GTCTTCACCA CTATCATGAT TCCATATAGG TCAGTGATCA TTCCCATCAA AAAGCTGAGC
    AACGCGATCA TCACGTCAAA CCCTTGGATC TGTGTATCAG GAGAACTAGG AGACACGGGA
    GTAATGCAGA TTCCCAAAAA CCTCCTTGAA ATGACTTTTG AATGCCAGAA TCTGGGGAAG
    CTGACGACTG TCCAGATCGG TCACGATAAC TCAGGACTGT TAGCCAAATG GCTAGTGGAT
    TGTGTCATGG TCAGAAATGA AATCACAGGA CATACATATA GGTTCCCATG TGGGCGGTGG
    CTGGGGAAAG GCATTGATGA TGGAAGTCTG GAGAGAATTC TCATTGGAGA ATTGATGACA
    TCAGCAGCAG ATGAGGATCT AGTAAAGCAG TGTCGGACTC CACCCCAGCA GAAATCACCC
    ACTGTGGCCA GGAGACTGAG CATCACATCA CTGACAGGAA AAACTACCAA ACCCAATGCA
    GGACAGATCC AAGAAGGAAT TGGAGAAGCT GTGAACAACA TTGTGAAACA TTTTCACAAA
    CCTGAAAAAG AGAGAGGAAG CCTCACTGTG CTGCTGTGTG GAGAGAGCGG CTTAGTTGCG
    GCGCTTGAGC AAGTTTTCCA CCATGGCTTC AAATCAGCTC GCATCTTTCA CAAGAATGTC
    TTCATCTGGG ACTTCATAGA GAGAGCAGTT GCTTATTTTG AAACCACTGA CCAGATTCTA
    GATAATGAAG ATGATGTCCT CATTCAGAAA TCATCCTGCA AAACCTTCTG CCACTATGTA
    AATGCTATTA ATACTGCACC CCGGAATATT GGGAAGGATG GCAAATTCCA GATCTTAGTT
    TGCCTTGGAA CACGAGATCG CCTGCTCCCA CAGTGGATTC CTCTGTTAGC CGAGTGTCCT
    GCCATCACCC GAATGTATGA GGAGAGCGCC CTCCTGCGAG ATCACATGAC GGTCAACTCC
    CTTATCCGCA TTCTGCAGAC CATCCAGGAC TTCACCATAG TCTTAGAAGG CTCGCTCATC
    AAAGGAGTGG ATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90786
    Clone ID Related Accession (Same CDS sequence) XM_015471176.1 , XM_015471176.2
    Accession Version XM_015471176.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3978bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DENN domain-containing protein 5B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471176.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGA AATACATGCA AGAGGCACGA
    AGTTTAGGAA AAAACCTGAG GCAACCCAAA TTGTCAGACC TCTCTCCTGC GGTGATTGCA
    CAAACCAACT GGAAATTTGT AGAAGGCTTA TTAAAAGAAT GTAGAATGAA GACGAAACGC
    ATGTTGGTGG AGAAGATGGG ACACGAAGCA GTGGAACTGG GCCATGGAGA AGCCAACATA
    ACTGGCTTGG AGGAGAACAC CCTGATTGCC AGCCTCTGTG ACCTGTTGGA AAGGATATGG
    AGCCACGGCT TGCAAGTCAA GCAGGGGAAG TCAGCTTTGT GGTCACATTT AATTCAGTTT
    CAGGACAGAG AAGAGAAACA AGAGCACCTT GCAGAGTCAC CAGTTGCCCT TGGACCAGAA
    AGAAGAAAAT CTGACTCAGG AGTTATGTTG CCAACCCTCA GGGTCTCTCT TATCCAAGAC
    ATGAGGCATA TTCAAAACAT GAGTGAGATC AAGACTGATG TCGGACGAGC TCGAGCATGG
    ATAAGATTGT CTCTAGAAAA GAAGGTCTTG TCCCAGCATC TCAAGCAGTT GCTCTCTAAC
    CAGCTGCTCA CAAAGAAGCT TTATAAACGT TATGCTTTTC TCCGTTGTGA AGAAGAAAGA
    GAGCAGTTTC TTTACCATCT TCTTTCCCTC AATGCTGTGG ACTATTTCTG CTTCACCAGT
    GTCTTCACCA CTATCATGAT TCCATATAGG TCAGTGATCA TTCCCATCAA AAAGCTGAGC
    AACGCGATCA TCACGTCAAA CCCTTGGATC TGTGTATCAG GAGAACTAGG AGACACGGGA
    GTAATGCAGA TTCCCAAAAA CCTCCTTGAA ATGACTTTTG AATGCCAGAA TCTGGGGAAG
    CTGACGACTG TCCAGATCGG TCACGATAAC TCAGGACTGT TAGCCAAATG GCTAGTGGAT
    TGTGTCATGG TCAGAAATGA AATCACAGGA CATACATATA GGTTCCCATG TGGGCGGTGG
    CTGGGGAAAG GCATTGATGA TGGAAGTCTG GAGAGAATTC TCATTGGAGA ATTGATGACA
    TCAGCAGCAG ATGAGGATCT AGTAAAGCAG TGTCGGACTC CACCCCAGCA GAAATCACCC
    ACTGTGGCCA GGAGACTGAG CATCACATCA CTGACAGGAA AAACTACCAA ACCCAATGCA
    GGACAGATCC AAGAAGGAAT TGGAGAAGCT GTGAACAACA TTGTGAAACA TTTTCACAAA
    CCTGAAAAAG AGAGAGGAAG CCTCACTGTG CTGCTGTGTG GAGAGAGCGG CTTAGTTGCG
    GCGCTTGAGC AAGTTTTCCA CCATGGCTTC AAATCAGCTC GCATCTTTCA CAAGAATGTC
    TTCATCTGGG ACTTCATAGA GAGAGCAGTT GCTTATTTTG AAACCACTGA CCAGATTCTA
    GATAATGAAG ATGATGTCCT CATTCAGAAA TCATCCTGCA AAACCTTCTG CCACTATGTA
    AATGCTATTA ATACTGCACC CCGGAATATT GGGAAGGATG GCAAATTCCA GATCTTAGTT
    TGCCTTGGAA CACGAGATCG CCTGCTCCCA CAGTGGATTC CTCTGTTAGC CGAGTGTCCT
    GCCATCACCC GAATGTATGA GGAGAGCGCC CTCCTGCGAG ATCACATGAC GGTCAACTCC
    CTTATCCGCA TTCTGCAGAC CATCCAGGAC TTCACCATAG TCTTAGAAGG CTCGCTCATC
    AAAGGAGTGG ATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326662.1
    CDS288..4265
    Translation

    Target ORF information:

    RefSeq Version XM_015471176.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DENN domain containing 5B (DENND5B), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471176.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCGGGA GCGGCGCGGC GCCCGGCCCG GCCTCGGGCT CCTCCCCGGC CGCCTGCCGC 
    TTTGCGCACT ACTTCGTGCT GTGCGGGATC GACGCGGACA GCGGGCTGGA GCCGGACGAG
    CTGGCGGTTT TGTACCAATG GCTAGAAGCA GATCGTCATG GCAAGAGCCA AGATGCTGCA
    AATACGACTT CAGGTGAAAA TTTTGACCAG AGTCCTTTGA GAAGAACATT CAAATCCAAA
    GTTCTGGCCC ACTATCCTCA GAATATAGAA TGGAACCCTT TCGATCAAGA TGCAGTAAAC
    ATGTTGTGCA TGCCTAAAGG GCTGTCTTTC AGAACACAAA CTGACAACAA AGACCCTCAG
    TTCCACTCAT TTATAATTAC CCGGGAAGAT GGATCTCGTA CCTACGGTTT TGTTCTCACT
    TTTTATGAAG AAGTTACAAG TAAGCAAATC TGCACAGCAA TGCAGACACT CTACCAGATG
    CACAATGCAG AGCATTACAG CAGCGTGTAC GCTTCATCCT CCTGCAGCAT GGATTCACTG
    GCGAGCAGTA TTGACGAGGG AGATACAACT TCCCTTTTGA AACTCCAGCG ATACAACTCC
    TATGACATCA GCAGAGACAC CCTGTATGTT TCAAAAAGTA TATGTTTGAT CACACCACTG
    CCTTTCATGC AGGCCTGCAA GAAATTCCTC ATCCAACTTT ACAAGGCAGT TACCTCACAG
    CAGCCACCAC CTTTGCCTCT TGAGAGCTAC ATCCATAATA TTCTCTATGA GGTGCCCCTT
    CCACCTCCAG GAAGATCACT GAAATTTTAT GGTGTTTATG AACCTGTCAT CTGCCAGAGG
    CCGGGGCTCA ATGAACTCCC CCTCTCCGAT TACCCTCTCC GAGAGGTCTT TGAGCTCCTG
    GGCCTAGAGA ACCTGGTGCA GGTATTTACC TGTGTTCTTC TGGAGATGCA GATCCTTCTC
    TATTCACAAG ATTATCAGCG CCTGATGACT GTGGCTGAAG GCATCACCAC ACTTCTGTTC
    CCATTTCAGT GGCAGCATGT TTATGTGCCC ATCCTCCCTG CTTCTCTGCT CCACTTTCTT
    GATGCTCCTG TCCCTTATCT GATGGGCCTT CAGTCAAAAG AAGGAACTGA CCGTTCTAAG
    CTAGAACTTC CTCAAGAGGC TAACTTGTGT TTTGTAGATA TTGACAACCA TTTTATTGAG
    TTGCCTGAAG AATTTCCACA GTTCCCCAAT AAAGTGGATT TTATCCAGGA ACTCTCTGAA
    GTTCTTCTTC AGTTTGGGAT CCCTCCTGAG GGCAGTCTAC ATTGCAGTGA GAGTGCCACC
    AAGCTCAAGA ATCTCGTTCT GAAAGACTTG GTCAATGACA AGAAGAATGG CAATGTCTCT
    GCAAATAGCA TCAGCATGTA TGAGTTACTG AAGGGCAACG AAACCATAGC TCGTCTCCAG
    GCTCTGGCCA AGCGGACTGG TGTGACTATG GAAAAAATGG ACCTCTCTGC CTCTCTGAGT
    GAAAAAGAAA AAGACTTAAA ACTGCAGTGT GAAGACGCAG AACTAAGGGA CTACCAGCTC
    AATGTTCAGC TCCGAGAGGT CTTTGCCAAC CGCTTTACAC AGATGTTTGC AGATTATGAA
    GCGTTTGTGA TTCAGACTGC CCAGGACCTG GAATCCTGGC TGACCAACCG GGAACAGATG
    CAAAACTTTG ATAAAGCTTC CTTCCTGTCT GACCAGCCAG AGCCGTACCT GCCATTTCTT
    TCACGCTTCA TTGAAACACA GATGTTTGCC ACCTTTATTG ATAACAAAAT TATGTCTCAA
    TGGGAGGAGA AAGATCCTTT GCTTCGAGTC TTTGACTCTC GGATTGAGAA GATAAGACTG
    TATAATGTAA GGGCACCCAC CTTGAGGACA TCTATTTACC AGAAATGCAG CACTTTAAAA
    GAAGCAGGTG AACGCTTTAG GCAGAGGCTA TCTGCTGCTG ATATTGTGGC ACCAGAACCT
    CATGATTTCT TTGCAGAAAG ACCACAAGTT GCTACCCAGT CAATTGAGCA GCGACTGATG
    AAAATGGATC ACACCGCAAT CCACCCACAT CTACTTGATA TGAAAATTGG TCAAGGCAAA
    TACGAGCAAG GATTTTTTCC AAAGTTACAG TCTGATGTCT TGGCAACAGG ACCAACTAAT
    AACAATCGCT GGGTGAGTCG GAGTGCCACT GCACAGCGCA GGAAAGACCG CCTTCGCCAG
    CATTCCGACC ACGTTGGGCT GGACAACGAC TTGAGGGAGA AATACATGCA AGAGGCACGA
    AGTTTAGGAA AAAACCTGAG GCAACCCAAA TTGTCAGACC TCTCTCCTGC GGTGATTGCA
    CAAACCAACT GGAAATTTGT AGAAGGCTTA TTAAAAGAAT GTAGAATGAA GACGAAACGC
    ATGTTGGTGG AGAAGATGGG ACACGAAGCA GTGGAACTGG GCCATGGAGA AGCCAACATA
    ACTGGCTTGG AGGAGAACAC CCTGATTGCC AGCCTCTGTG ACCTGTTGGA AAGGATATGG
    AGCCACGGCT TGCAAGTCAA GCAGGGGAAG TCAGCTTTGT GGTCACATTT AATTCAGTTT
    CAGGACAGAG AAGAGAAACA AGAGCACCTT GCAGAGTCAC CAGTTGCCCT TGGACCAGAA
    AGAAGAAAAT CTGACTCAGG AGTTATGTTG CCAACCCTCA GGGTCTCTCT TATCCAAGAC
    ATGAGGCATA TTCAAAACAT GAGTGAGATC AAGACTGATG TCGGACGAGC TCGAGCATGG
    ATAAGATTGT CTCTAGAAAA GAAGGTCTTG TCCCAGCATC TCAAGCAGTT GCTCTCTAAC
    CAGCTGCTCA CAAAGAAGCT TTATAAACGT TATGCTTTTC TCCGTTGTGA AGAAGAAAGA
    GAGCAGTTTC TTTACCATCT TCTTTCCCTC AATGCTGTGG ACTATTTCTG CTTCACCAGT
    GTCTTCACCA CTATCATGAT TCCATATAGG TCAGTGATCA TTCCCATCAA AAAGCTGAGC
    AACGCGATCA TCACGTCAAA CCCTTGGATC TGTGTATCAG GAGAACTAGG AGACACGGGA
    GTAATGCAGA TTCCCAAAAA CCTCCTTGAA ATGACTTTTG AATGCCAGAA TCTGGGGAAG
    CTGACGACTG TCCAGATCGG TCACGATAAC TCAGGACTGT TAGCCAAATG GCTAGTGGAT
    TGTGTCATGG TCAGAAATGA AATCACAGGA CATACATATA GGTTCCCATG TGGGCGGTGG
    CTGGGGAAAG GCATTGATGA TGGAAGTCTG GAGAGAATTC TCATTGGAGA ATTGATGACA
    TCAGCAGCAG ATGAGGATCT AGTAAAGCAG TGTCGGACTC CACCCCAGCA GAAATCACCC
    ACTGTGGCCA GGAGACTGAG CATCACATCA CTGACAGGAA AAACTACCAA ACCCAATGCA
    GGACAGATCC AAGAAGGAAT TGGAGAAGCT GTGAACAACA TTGTGAAACA TTTTCACAAA
    CCTGAAAAAG AGAGAGGAAG CCTCACTGTG CTGCTGTGTG GAGAGAGCGG CTTAGTTGCG
    GCGCTTGAGC AAGTTTTCCA CCATGGCTTC AAATCAGCTC GCATCTTTCA CAAGAATGTC
    TTCATCTGGG ACTTCATAGA GAGAGCAGTT GCTTATTTTG AAACCACTGA CCAGATTCTA
    GATAATGAAG ATGATGTCCT CATTCAGAAA TCATCCTGCA AAACCTTCTG CCACTATGTA
    AATGCTATTA ATACTGCACC CCGGAATATT GGGAAGGATG GCAAATTCCA GATCTTAGTT
    TGCCTTGGAA CACGAGATCG CCTGCTCCCA CAGTGGATTC CTCTGTTAGC CGAGTGTCCT
    GCCATCACCC GAATGTATGA GGAGAGCGCC CTCCTGCGAG ATCACATGAC GGTCAACTCC
    CTTATCCGCA TTCTGCAGAC CATCCAGGAC TTCACCATAG TCTTAGAAGG CTCGCTCATC
    AAAGGAGTGG ATGTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.