GIT2 cDNA ORF clone, Bos taurus(cattle)

The following GIT2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIT2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa27883 XM_005217841.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa49507 XM_010813922.3
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa49508 XM_010813923.3
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa224262 XM_024977224.1
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OBa38450 NM_001193101.1
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27875 XM_005217833.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27876 XM_005217834.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27877 XM_005217835.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27878 XM_005217836.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27879 XM_005217837.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27880 XM_005217838.4
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa224260 XM_024977222.1
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa224261 XM_024977223.1
Latest version!
Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa27877 XM_005217835.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27876 XM_005217834.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27875 XM_005217833.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27879 XM_005217837.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27878 XM_005217836.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa27880 XM_005217838.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa49507 XM_010813922.2 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa49508 XM_010813923.2 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa27883 XM_005217841.3 Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa27883
    Clone ID Related Accession (Same CDS sequence) XM_005217841.3 , XM_005217841.4
    Accession Version XM_005217841.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217898.1
    CDS149..1564
    Translation

    Target ORF information:

    RefSeq Version XM_005217841.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217841.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27883
    Clone ID Related Accession (Same CDS sequence) XM_005217841.3 , XM_005217841.4
    Accession Version XM_005217841.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1416bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217841.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217898.1
    CDS151..1566
    Translation

    Target ORF information:

    RefSeq Version XM_005217841.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217841.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGTTGCTTGG AAAAGATGCT AATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49507
    Clone ID Related Accession (Same CDS sequence) XM_010813922.2 , XM_010813922.3
    Accession Version XM_010813922.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813922.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGGCATCCCG GCTGGAGAAG CAGAATAGCA CACCTGAGAG TGACTACGAC
    AACACTCCCA ACGACAGGGA CCTGGATGAC ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG
    AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC CCAGACACGG CAGAGCCCCA TGTGGCCCCT
    AGCCCCATCC TCCCCAGCAC CGAAGACGTC ATCCGGAAGA CAGAGCAGAT CACCAAAAAC
    ATACAGGAGC TCCTAAGGGC AGCCCAAGAA AATAAACATG ACAGTTATAT TCCCTGCTCA
    GAGAGGATAC ATGTGGCTGT TACAGAAATG GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC
    GACATGGTGA GGACCTCCCT CCGCTTACTG ACGTCCAGTG CCTACCGACT GCAGTCAGAA
    TGCAAGAAGA CCCTCCCGGG GGACCCGGGC CCGCCCACGG ACATCCAGCT GGTCACGCAG
    CAGGTCATCC AGTGTGCCTA CGACATCGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812224.1
    CDS149..2089
    Translation

    Target ORF information:

    RefSeq Version XM_010813922.2
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813922.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGGCATCCCG GCTGGAGAAG CAGAATAGCA CACCTGAGAG TGACTACGAC
    AACACTCCCA ACGACAGGGA CCTGGATGAC ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG
    AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC CCAGACACGG CAGAGCCCCA TGTGGCCCCT
    AGCCCCATCC TCCCCAGCAC CGAAGACGTC ATCCGGAAGA CAGAGCAGAT CACCAAAAAC
    ATACAGGAGC TCCTAAGGGC AGCCCAAGAA AATAAACATG ACAGTTATAT TCCCTGCTCA
    GAGAGGATAC ATGTGGCTGT TACAGAAATG GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC
    GACATGGTGA GGACCTCCCT CCGCTTACTG ACGTCCAGTG CCTACCGACT GCAGTCAGAA
    TGCAAGAAGA CCCTCCCGGG GGACCCGGGC CCGCCCACGG ACATCCAGCT GGTCACGCAG
    CAGGTCATCC AGTGTGCCTA CGACATCGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49507
    Clone ID Related Accession (Same CDS sequence) XM_010813922.2 , XM_010813922.3
    Accession Version XM_010813922.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1941bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813922.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGGCATCCCG GCTGGAGAAG CAGAATAGCA CACCTGAGAG TGACTACGAC
    AACACTCCCA ACGACAGGGA CCTGGATGAC ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG
    AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC CCAGACACGG CAGAGCCCCA TGTGGCCCCT
    AGCCCCATCC TCCCCAGCAC CGAAGACGTC ATCCGGAAGA CAGAGCAGAT CACCAAAAAC
    ATACAGGAGC TCCTAAGGGC AGCCCAAGAA AATAAACATG ACAGTTATAT TCCCTGCTCA
    GAGAGGATAC ATGTGGCTGT TACAGAAATG GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC
    GACATGGTGA GGACCTCCCT CCGCTTACTG ACGTCCAGTG CCTACCGACT GCAGTCAGAA
    TGCAAGAAGA CCCTCCCGGG GGACCCGGGC CCGCCCACGG ACATCCAGCT GGTCACGCAG
    CAGGTCATCC AGTGTGCCTA CGACATCGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812224.1
    CDS147..2087
    Translation

    Target ORF information:

    RefSeq Version XM_010813922.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813922.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGGCATCCCG GCTGGAGAAG CAGAATAGCA CACCTGAGAG TGACTACGAC
    AACACTCCCA ACGACAGGGA CCTGGATGAC ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG
    AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC CCAGACACGG CAGAGCCCCA TGTGGCCCCT
    AGCCCCATCC TCCCCAGCAC CGAAGACGTC ATCCGGAAGA CAGAGCAGAT CACCAAAAAC
    ATACAGGAGC TCCTAAGGGC AGCCCAAGAA AATAAACATG ACAGTTATAT TCCCTGCTCA
    GAGAGGATAC ATGTGGCTGT TACAGAAATG GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC
    GACATGGTGA GGACCTCCCT CCGCTTACTG ACGTCCAGTG CCTACCGACT GCAGTCAGAA
    TGCAAGAAGA CCCTCCCGGG GGACCCGGGC CCGCCCACGG ACATCCAGCT GGTCACGCAG
    CAGGTCATCC AGTGTGCCTA CGACATCGCC AAGGCCGCCA AGCAGCTGGT CACCATCACC
    ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49508
    Clone ID Related Accession (Same CDS sequence) XM_010813923.2 , XM_010813923.3
    Accession Version XM_010813923.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGGCATCCCG GCTGGAGAAG
    CAGAATAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACAGGGA CCTGGATGAC
    ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC
    CCAGACACGG CAGAGCCCCA TGTGGCCCCT AGCCCCATCC TCCCCAGCAC CGAAGACGTC
    ATCCGGAAGA CAGAGCAGAT CACCAAAAAC ATACAGGAGC TCCTAAGGGC AGCCCAAGAA
    AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ATGTGGCTGT TACAGAAATG
    GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC GACATGGTGA GGACCTCCCT CCGCTTACTG
    ACGTCCAGTG CCTACCGACT GCAGTCAGAA TGCAAGAAGA CCCTCCCGGG GGACCCGGGC
    CCGCCCACGG ACATCCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCCTA CGACATCGCC
    AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812225.1
    CDS149..1939
    Translation

    Target ORF information:

    RefSeq Version XM_010813923.2
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813923.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGGCATCCCG GCTGGAGAAG
    CAGAATAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACAGGGA CCTGGATGAC
    ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC
    CCAGACACGG CAGAGCCCCA TGTGGCCCCT AGCCCCATCC TCCCCAGCAC CGAAGACGTC
    ATCCGGAAGA CAGAGCAGAT CACCAAAAAC ATACAGGAGC TCCTAAGGGC AGCCCAAGAA
    AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ATGTGGCTGT TACAGAAATG
    GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC GACATGGTGA GGACCTCCCT CCGCTTACTG
    ACGTCCAGTG CCTACCGACT GCAGTCAGAA TGCAAGAAGA CCCTCCCGGG GGACCCGGGC
    CCGCCCACGG ACATCCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCCTA CGACATCGCC
    AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49508
    Clone ID Related Accession (Same CDS sequence) XM_010813923.2 , XM_010813923.3
    Accession Version XM_010813923.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1791bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGGCATCCCG GCTGGAGAAG
    CAGAATAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACAGGGA CCTGGATGAC
    ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC
    CCAGACACGG CAGAGCCCCA TGTGGCCCCT AGCCCCATCC TCCCCAGCAC CGAAGACGTC
    ATCCGGAAGA CAGAGCAGAT CACCAAAAAC ATACAGGAGC TCCTAAGGGC AGCCCAAGAA
    AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ATGTGGCTGT TACAGAAATG
    GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC GACATGGTGA GGACCTCCCT CCGCTTACTG
    ACGTCCAGTG CCTACCGACT GCAGTCAGAA TGCAAGAAGA CCCTCCCGGG GGACCCGGGC
    CCGCCCACGG ACATCCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCCTA CGACATCGCC
    AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812225.1
    CDS147..1937
    Translation

    Target ORF information:

    RefSeq Version XM_010813923.3
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813923.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGGCATCCCG GCTGGAGAAG
    CAGAATAGCA CACCTGAGAG TGACTACGAC AACACTCCCA ACGACAGGGA CCTGGATGAC
    ATGGGGTCAA GCAGGAAAGG AAGGCAGAGG AGCTCGGTGT GGCAGAGTGA CAGCTCAGGC
    CCAGACACGG CAGAGCCCCA TGTGGCCCCT AGCCCCATCC TCCCCAGCAC CGAAGACGTC
    ATCCGGAAGA CAGAGCAGAT CACCAAAAAC ATACAGGAGC TCCTAAGGGC AGCCCAAGAA
    AATAAACATG ACAGTTATAT TCCCTGCTCA GAGAGGATAC ATGTGGCTGT TACAGAAATG
    GCAGCGTTAT TCCCCAAAAA ACCCAAGTCC GACATGGTGA GGACCTCCCT CCGCTTACTG
    ACGTCCAGTG CCTACCGACT GCAGTCAGAA TGCAAGAAGA CCCTCCCGGG GGACCCGGGC
    CCGCCCACGG ACATCCAGCT GGTCACGCAG CAGGTCATCC AGTGTGCCTA CGACATCGCC
    AAGGCCGCCA AGCAGCTGGT CACCATCACC ACCAAAGAGA ACAACAACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224262
    Clone ID Related Accession (Same CDS sequence) XM_024977224.1
    Accession Version XM_024977224.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1371bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832992.1
    CDS145..1515
    Translation

    Target ORF information:

    RefSeq Version XM_024977224.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977224.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGTTG CTTGGAAAAG ATGCTAATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa38450
    Clone ID Related Accession (Same CDS sequence) NM_001193101.1
    Accession Version NM_001193101.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000017.1. On Jul 17, 2010 this sequence version replaced XM_868170.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571259.34670.1, SRR5571256.159.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CATCGGGAGG AGTGCGTTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCAACACT GTCCTGGTCG AGGGACGAGA GCACCCGAAG GGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180030.1
    CDS102..2381
    Translation

    Target ORF information:

    RefSeq Version NM_001193101.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193101.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CATCGGGAGG AGTGCGTTTG TGACCTCCTC TTCATCTCTG
    CCTTCCTTCC CCTCAACACT GTCCTGGTCG AGGGACGAGA GCACCCGAAG GGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27875
    Clone ID Related Accession (Same CDS sequence) XM_005217833.4 , XM_005217833.3
    Accession Version XM_005217833.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217833.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGA GCCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTTTG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AACAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC AGGATCGGGT
    CAGAAACCCT ATCTCCCAAT GGGAGAAGTG AACCATCCCG AAGAGAGCAG GACGAGACTC
    CAGCCTTTCC CGGCGCACAT CGGGAGGAGT GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CAACACTGTC CTGGTCGAGG GACGAGAGCA CCCGAAGGGC ATCCCGGCTG
    GAGAAGCAGA ATAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CAGGGACCTG
    GATGACATGG GGTCAAGCAG GAAAGGAAGG CAGAGGAGCT CGGTGTGGCA GAGTGACAGC
    TCAGGCCCAG ACACGGCAGA GCCCCATGTG GCCCCTAGCC CCATCCTCCC CAGCACCGAA
    GACGTCATCC GGAAGACAGA GCAGATCACC AAAAACATAC AGGAGCTCCT AAGGGCAGCC
    CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACATGT GGCTGTTACA
    GAAATGGCAG CGTTATTCCC CAAAAAACCC AAGTCCGACA TGGTGAGGAC CTCCCTCCGC
    TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAATGCA AGAAGACCCT CCCGGGGGAC
    CCGGGCCCGC CCACGGACAT CCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCCTACGAC
    ATCGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217890.1
    CDS148..2424
    Translation

    Target ORF information:

    RefSeq Version XM_005217833.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217833.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGA GCCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTTTG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AACAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC AGGATCGGGT
    CAGAAACCCT ATCTCCCAAT GGGAGAAGTG AACCATCCCG AAGAGAGCAG GACGAGACTC
    CAGCCTTTCC CGGCGCACAT CGGGAGGAGT GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CAACACTGTC CTGGTCGAGG GACGAGAGCA CCCGAAGGGC ATCCCGGCTG
    GAGAAGCAGA ATAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CAGGGACCTG
    GATGACATGG GGTCAAGCAG GAAAGGAAGG CAGAGGAGCT CGGTGTGGCA GAGTGACAGC
    TCAGGCCCAG ACACGGCAGA GCCCCATGTG GCCCCTAGCC CCATCCTCCC CAGCACCGAA
    GACGTCATCC GGAAGACAGA GCAGATCACC AAAAACATAC AGGAGCTCCT AAGGGCAGCC
    CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACATGT GGCTGTTACA
    GAAATGGCAG CGTTATTCCC CAAAAAACCC AAGTCCGACA TGGTGAGGAC CTCCCTCCGC
    TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAATGCA AGAAGACCCT CCCGGGGGAC
    CCGGGCCCGC CCACGGACAT CCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCCTACGAC
    ATCGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27875
    Clone ID Related Accession (Same CDS sequence) XM_005217833.4 , XM_005217833.3
    Accession Version XM_005217833.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2277bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGA GCCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTTTG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AACAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC AGGATCGGGT
    CAGAAACCCT ATCTCCCAAT GGGAGAAGTG AACCATCCCG AAGAGAGCAG GACGAGACTC
    CAGCCTTTCC CGGCGCACAT CGGGAGGAGT GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CAACACTGTC CTGGTCGAGG GACGAGAGCA CCCGAAGGGC ATCCCGGCTG
    GAGAAGCAGA ATAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CAGGGACCTG
    GATGACATGG GGTCAAGCAG GAAAGGAAGG CAGAGGAGCT CGGTGTGGCA GAGTGACAGC
    TCAGGCCCAG ACACGGCAGA GCCCCATGTG GCCCCTAGCC CCATCCTCCC CAGCACCGAA
    GACGTCATCC GGAAGACAGA GCAGATCACC AAAAACATAC AGGAGCTCCT AAGGGCAGCC
    CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACATGT GGCTGTTACA
    GAAATGGCAG CGTTATTCCC CAAAAAACCC AAGTCCGACA TGGTGAGGAC CTCCCTCCGC
    TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAATGCA AGAAGACCCT CCCGGGGGAC
    CCGGGCCCGC CCACGGACAT CCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCCTACGAC
    ATCGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217890.1
    CDS149..2425
    Translation

    Target ORF information:

    RefSeq Version XM_005217833.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217833.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGA GCCTAGATTC AGATTTATCA
    GATGGACCAG TCACTGTGCA GGAATTTTTG GAGGTCAAAA ACGCTCTAGT GGCTTCTGAG
    GCCAAGATAC AACAGCTAAT GAAGGTGAAT AACAACTTGA GTGACGAGCT GAGAATTATG
    CAGAAAAAGC TTCAAACACT CCAGAGTGAA AATTCGAACC TCAGGAAACA GGCCACAACC
    AATATATATC AGGTGCAAAC TGGTTCTGAG TACACAGACA CTTCCAACCA CTCTTCCTTA
    AAGCGACGTC CGTCTGCCCG GGGCAGTAGG CCCATGTCCA TGTACGAGAC AGGATCGGGT
    CAGAAACCCT ATCTCCCAAT GGGAGAAGTG AACCATCCCG AAGAGAGCAG GACGAGACTC
    CAGCCTTTCC CGGCGCACAT CGGGAGGAGT GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT
    TCCTTCCCCT CAACACTGTC CTGGTCGAGG GACGAGAGCA CCCGAAGGGC ATCCCGGCTG
    GAGAAGCAGA ATAGCACACC TGAGAGTGAC TACGACAACA CTCCCAACGA CAGGGACCTG
    GATGACATGG GGTCAAGCAG GAAAGGAAGG CAGAGGAGCT CGGTGTGGCA GAGTGACAGC
    TCAGGCCCAG ACACGGCAGA GCCCCATGTG GCCCCTAGCC CCATCCTCCC CAGCACCGAA
    GACGTCATCC GGAAGACAGA GCAGATCACC AAAAACATAC AGGAGCTCCT AAGGGCAGCC
    CAAGAAAATA AACATGACAG TTATATTCCC TGCTCAGAGA GGATACATGT GGCTGTTACA
    GAAATGGCAG CGTTATTCCC CAAAAAACCC AAGTCCGACA TGGTGAGGAC CTCCCTCCGC
    TTACTGACGT CCAGTGCCTA CCGACTGCAG TCAGAATGCA AGAAGACCCT CCCGGGGGAC
    CCGGGCCCGC CCACGGACAT CCAGCTGGTC ACGCAGCAGG TCATCCAGTG TGCCTACGAC
    ATCGCCAAGG CCGCCAAGCA GCTGGTCACC ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27876
    Clone ID Related Accession (Same CDS sequence) XM_005217834.3 , XM_005217834.4
    Accession Version XM_005217834.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC
    AGGAAACAGG CCACAACCAA TATATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT
    TCCAACCACT CTTCCTTAAA GCGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG
    TACGAGACAG GATCGGGTCA GAAACCCTAT CTCCCAATGG GAGAAGTGAA CCATCCCGAA
    GAGAGCAGGA CGAGACTCCA GCCTTTCCCG GCGCACATCG GGAGGAGTGC GTTTGTGACC
    TCCTCTTCAT CTCTGCCTTC CTTCCCCTCA ACACTGTCCT GGTCGAGGGA CGAGAGCACC
    CGAAGGGCAT CCCGGCTGGA GAAGCAGAAT AGCACACCTG AGAGTGACTA CGACAACACT
    CCCAACGACA GGGACCTGGA TGACATGGGG TCAAGCAGGA AAGGAAGGCA GAGGAGCTCG
    GTGTGGCAGA GTGACAGCTC AGGCCCAGAC ACGGCAGAGC CCCATGTGGC CCCTAGCCCC
    ATCCTCCCCA GCACCGAAGA CGTCATCCGG AAGACAGAGC AGATCACCAA AAACATACAG
    GAGCTCCTAA GGGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG
    ATACATGTGG CTGTTACAGA AATGGCAGCG TTATTCCCCA AAAAACCCAA GTCCGACATG
    GTGAGGACCT CCCTCCGCTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAATGCAAG
    AAGACCCTCC CGGGGGACCC GGGCCCGCCC ACGGACATCC AGCTGGTCAC GCAGCAGGTC
    ATCCAGTGTG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCAAA
    GAGAACAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217891.1
    CDS149..2383
    Translation

    Target ORF information:

    RefSeq Version XM_005217834.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217834.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC
    AGGAAACAGG CCACAACCAA TATATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT
    TCCAACCACT CTTCCTTAAA GCGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG
    TACGAGACAG GATCGGGTCA GAAACCCTAT CTCCCAATGG GAGAAGTGAA CCATCCCGAA
    GAGAGCAGGA CGAGACTCCA GCCTTTCCCG GCGCACATCG GGAGGAGTGC GTTTGTGACC
    TCCTCTTCAT CTCTGCCTTC CTTCCCCTCA ACACTGTCCT GGTCGAGGGA CGAGAGCACC
    CGAAGGGCAT CCCGGCTGGA GAAGCAGAAT AGCACACCTG AGAGTGACTA CGACAACACT
    CCCAACGACA GGGACCTGGA TGACATGGGG TCAAGCAGGA AAGGAAGGCA GAGGAGCTCG
    GTGTGGCAGA GTGACAGCTC AGGCCCAGAC ACGGCAGAGC CCCATGTGGC CCCTAGCCCC
    ATCCTCCCCA GCACCGAAGA CGTCATCCGG AAGACAGAGC AGATCACCAA AAACATACAG
    GAGCTCCTAA GGGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG
    ATACATGTGG CTGTTACAGA AATGGCAGCG TTATTCCCCA AAAAACCCAA GTCCGACATG
    GTGAGGACCT CCCTCCGCTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAATGCAAG
    AAGACCCTCC CGGGGGACCC GGGCCCGCCC ACGGACATCC AGCTGGTCAC GCAGCAGGTC
    ATCCAGTGTG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCAAA
    GAGAACAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27876
    Clone ID Related Accession (Same CDS sequence) XM_005217834.3 , XM_005217834.4
    Accession Version XM_005217834.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2235bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC
    AGGAAACAGG CCACAACCAA TATATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT
    TCCAACCACT CTTCCTTAAA GCGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG
    TACGAGACAG GATCGGGTCA GAAACCCTAT CTCCCAATGG GAGAAGTGAA CCATCCCGAA
    GAGAGCAGGA CGAGACTCCA GCCTTTCCCG GCGCACATCG GGAGGAGTGC GTTTGTGACC
    TCCTCTTCAT CTCTGCCTTC CTTCCCCTCA ACACTGTCCT GGTCGAGGGA CGAGAGCACC
    CGAAGGGCAT CCCGGCTGGA GAAGCAGAAT AGCACACCTG AGAGTGACTA CGACAACACT
    CCCAACGACA GGGACCTGGA TGACATGGGG TCAAGCAGGA AAGGAAGGCA GAGGAGCTCG
    GTGTGGCAGA GTGACAGCTC AGGCCCAGAC ACGGCAGAGC CCCATGTGGC CCCTAGCCCC
    ATCCTCCCCA GCACCGAAGA CGTCATCCGG AAGACAGAGC AGATCACCAA AAACATACAG
    GAGCTCCTAA GGGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG
    ATACATGTGG CTGTTACAGA AATGGCAGCG TTATTCCCCA AAAAACCCAA GTCCGACATG
    GTGAGGACCT CCCTCCGCTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAATGCAAG
    AAGACCCTCC CGGGGGACCC GGGCCCGCCC ACGGACATCC AGCTGGTCAC GCAGCAGGTC
    ATCCAGTGTG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCAAA
    GAGAACAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217891.1
    CDS148..2382
    Translation

    Target ORF information:

    RefSeq Version XM_005217834.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217834.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGCTT CAAACACTCC AGAGTGAAAA TTCGAACCTC
    AGGAAACAGG CCACAACCAA TATATATCAG GTGCAAACTG GTTCTGAGTA CACAGACACT
    TCCAACCACT CTTCCTTAAA GCGACGTCCG TCTGCCCGGG GCAGTAGGCC CATGTCCATG
    TACGAGACAG GATCGGGTCA GAAACCCTAT CTCCCAATGG GAGAAGTGAA CCATCCCGAA
    GAGAGCAGGA CGAGACTCCA GCCTTTCCCG GCGCACATCG GGAGGAGTGC GTTTGTGACC
    TCCTCTTCAT CTCTGCCTTC CTTCCCCTCA ACACTGTCCT GGTCGAGGGA CGAGAGCACC
    CGAAGGGCAT CCCGGCTGGA GAAGCAGAAT AGCACACCTG AGAGTGACTA CGACAACACT
    CCCAACGACA GGGACCTGGA TGACATGGGG TCAAGCAGGA AAGGAAGGCA GAGGAGCTCG
    GTGTGGCAGA GTGACAGCTC AGGCCCAGAC ACGGCAGAGC CCCATGTGGC CCCTAGCCCC
    ATCCTCCCCA GCACCGAAGA CGTCATCCGG AAGACAGAGC AGATCACCAA AAACATACAG
    GAGCTCCTAA GGGCAGCCCA AGAAAATAAA CATGACAGTT ATATTCCCTG CTCAGAGAGG
    ATACATGTGG CTGTTACAGA AATGGCAGCG TTATTCCCCA AAAAACCCAA GTCCGACATG
    GTGAGGACCT CCCTCCGCTT ACTGACGTCC AGTGCCTACC GACTGCAGTC AGAATGCAAG
    AAGACCCTCC CGGGGGACCC GGGCCCGCCC ACGGACATCC AGCTGGTCAC GCAGCAGGTC
    ATCCAGTGTG CCTACGACAT CGCCAAGGCC GCCAAGCAGC TGGTCACCAT CACCACCAAA
    GAGAACAACA ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27877
    Clone ID Related Accession (Same CDS sequence) XM_005217835.3 , XM_005217835.4
    Accession Version XM_005217835.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217892.1
    CDS149..2338
    Translation

    Target ORF information:

    RefSeq Version XM_005217835.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217835.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27877
    Clone ID Related Accession (Same CDS sequence) XM_005217835.3 , XM_005217835.4
    Accession Version XM_005217835.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217892.1
    CDS148..2337
    Translation

    Target ORF information:

    RefSeq Version XM_005217835.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217835.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGTG AATAACAACT TGAGTGACGA GCTGAGAATT
    ATGCAGAAAA AGCTTCAAAC ACTCCAGAGT GAAAATTCGA ACCTCAGGAA ACAGGCCACA
    ACCAATATAT ATCAGGTGCA AACTGGTTCT GAGTACACAG ACACTTCCAA CCACTCTTCC
    TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT AGGCCCATGT CCATGTACGA GACAGGATCG
    GGTCAGAAAC CCTATCTCCC AATGGGAGAA GTGAACCATC CCGAAGAGAG CAGGACGAGA
    CTCCAGCCTT TCCCGGCGCA CGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27878
    Clone ID Related Accession (Same CDS sequence) XM_005217836.3 , XM_005217836.4
    Accession Version XM_005217836.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217836.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CATCGGGAGG
    AGTGCGTTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCAACACT GTCCTGGTCG
    AGGGACGAGA GCACCCGAAG GGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217893.1
    CDS149..2278
    Translation

    Target ORF information:

    RefSeq Version XM_005217836.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217836.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CATCGGGAGG
    AGTGCGTTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCAACACT GTCCTGGTCG
    AGGGACGAGA GCACCCGAAG GGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27878
    Clone ID Related Accession (Same CDS sequence) XM_005217836.3 , XM_005217836.4
    Accession Version XM_005217836.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217836.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CATCGGGAGG
    AGTGCGTTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCAACACT GTCCTGGTCG
    AGGGACGAGA GCACCCGAAG GGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217893.1
    CDS148..2277
    Translation

    Target ORF information:

    RefSeq Version XM_005217836.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217836.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CATCGGGAGG
    AGTGCGTTTG TGACCTCCTC TTCATCTCTG CCTTCCTTCC CCTCAACACT GTCCTGGTCG
    AGGGACGAGA GCACCCGAAG GGCATCCCGG CTGGAGAAGC AGAATAGCAC ACCTGAGAGT
    GACTACGACA ACACTCCCAA CGACAGGGAC CTGGATGACA TGGGGTCAAG CAGGAAAGGA
    AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC AGCTCAGGCC CAGACACGGC AGAGCCCCAT
    GTGGCCCCTA GCCCCATCCT CCCCAGCACC GAAGACGTCA TCCGGAAGAC AGAGCAGATC
    ACCAAAAACA TACAGGAGCT CCTAAGGGCA GCCCAAGAAA ATAAACATGA CAGTTATATT
    CCCTGCTCAG AGAGGATACA TGTGGCTGTT ACAGAAATGG CAGCGTTATT CCCCAAAAAA
    CCCAAGTCCG ACATGGTGAG GACCTCCCTC CGCTTACTGA CGTCCAGTGC CTACCGACTG
    CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG GACCCGGGCC CGCCCACGGA CATCCAGCTG
    GTCACGCAGC AGGTCATCCA GTGTGCCTAC GACATCGCCA AGGCCGCCAA GCAGCTGGTC
    ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27879
    Clone ID Related Accession (Same CDS sequence) XM_005217837.4 , XM_005217837.3
    Accession Version XM_005217837.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217837.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC AGGATCGGGT CAGAAACCCT ATCTCCCAAT GGGAGAAGTG
    AACCATCCCG AAGAGAGCAG GACGAGACTC CAGCCTTTCC CGGCGCACAT CGGGAGGAGT
    GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CAACACTGTC CTGGTCGAGG
    GACGAGAGCA CCCGAAGGGC ATCCCGGCTG GAGAAGCAGA ATAGCACACC TGAGAGTGAC
    TACGACAACA CTCCCAACGA CAGGGACCTG GATGACATGG GGTCAAGCAG GAAAGGAAGG
    CAGAGGAGCT CGGTGTGGCA GAGTGACAGC TCAGGCCCAG ACACGGCAGA GCCCCATGTG
    GCCCCTAGCC CCATCCTCCC CAGCACCGAA GACGTCATCC GGAAGACAGA GCAGATCACC
    AAAAACATAC AGGAGCTCCT AAGGGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC
    TGCTCAGAGA GGATACATGT GGCTGTTACA GAAATGGCAG CGTTATTCCC CAAAAAACCC
    AAGTCCGACA TGGTGAGGAC CTCCCTCCGC TTACTGACGT CCAGTGCCTA CCGACTGCAG
    TCAGAATGCA AGAAGACCCT CCCGGGGGAC CCGGGCCCGC CCACGGACAT CCAGCTGGTC
    ACGCAGCAGG TCATCCAGTG TGCCTACGAC ATCGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217894.1
    CDS149..2275
    Translation

    Target ORF information:

    RefSeq Version XM_005217837.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217837.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC AGGATCGGGT CAGAAACCCT ATCTCCCAAT GGGAGAAGTG
    AACCATCCCG AAGAGAGCAG GACGAGACTC CAGCCTTTCC CGGCGCACAT CGGGAGGAGT
    GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CAACACTGTC CTGGTCGAGG
    GACGAGAGCA CCCGAAGGGC ATCCCGGCTG GAGAAGCAGA ATAGCACACC TGAGAGTGAC
    TACGACAACA CTCCCAACGA CAGGGACCTG GATGACATGG GGTCAAGCAG GAAAGGAAGG
    CAGAGGAGCT CGGTGTGGCA GAGTGACAGC TCAGGCCCAG ACACGGCAGA GCCCCATGTG
    GCCCCTAGCC CCATCCTCCC CAGCACCGAA GACGTCATCC GGAAGACAGA GCAGATCACC
    AAAAACATAC AGGAGCTCCT AAGGGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC
    TGCTCAGAGA GGATACATGT GGCTGTTACA GAAATGGCAG CGTTATTCCC CAAAAAACCC
    AAGTCCGACA TGGTGAGGAC CTCCCTCCGC TTACTGACGT CCAGTGCCTA CCGACTGCAG
    TCAGAATGCA AGAAGACCCT CCCGGGGGAC CCGGGCCCGC CCACGGACAT CCAGCTGGTC
    ACGCAGCAGG TCATCCAGTG TGCCTACGAC ATCGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27879
    Clone ID Related Accession (Same CDS sequence) XM_005217837.4 , XM_005217837.3
    Accession Version XM_005217837.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2127bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC AGGATCGGGT CAGAAACCCT ATCTCCCAAT GGGAGAAGTG
    AACCATCCCG AAGAGAGCAG GACGAGACTC CAGCCTTTCC CGGCGCACAT CGGGAGGAGT
    GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CAACACTGTC CTGGTCGAGG
    GACGAGAGCA CCCGAAGGGC ATCCCGGCTG GAGAAGCAGA ATAGCACACC TGAGAGTGAC
    TACGACAACA CTCCCAACGA CAGGGACCTG GATGACATGG GGTCAAGCAG GAAAGGAAGG
    CAGAGGAGCT CGGTGTGGCA GAGTGACAGC TCAGGCCCAG ACACGGCAGA GCCCCATGTG
    GCCCCTAGCC CCATCCTCCC CAGCACCGAA GACGTCATCC GGAAGACAGA GCAGATCACC
    AAAAACATAC AGGAGCTCCT AAGGGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC
    TGCTCAGAGA GGATACATGT GGCTGTTACA GAAATGGCAG CGTTATTCCC CAAAAAACCC
    AAGTCCGACA TGGTGAGGAC CTCCCTCCGC TTACTGACGT CCAGTGCCTA CCGACTGCAG
    TCAGAATGCA AGAAGACCCT CCCGGGGGAC CCGGGCCCGC CCACGGACAT CCAGCTGGTC
    ACGCAGCAGG TCATCCAGTG TGCCTACGAC ATCGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217894.1
    CDS149..2275
    Translation

    Target ORF information:

    RefSeq Version XM_005217837.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217837.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGC TTCAAACACT CCAGAGTGAA
    AATTCGAACC TCAGGAAACA GGCCACAACC AATATATATC AGGTGCAAAC TGGTTCTGAG
    TACACAGACA CTTCCAACCA CTCTTCCTTA AAGCGACGTC CGTCTGCCCG GGGCAGTAGG
    CCCATGTCCA TGTACGAGAC AGGATCGGGT CAGAAACCCT ATCTCCCAAT GGGAGAAGTG
    AACCATCCCG AAGAGAGCAG GACGAGACTC CAGCCTTTCC CGGCGCACAT CGGGAGGAGT
    GCGTTTGTGA CCTCCTCTTC ATCTCTGCCT TCCTTCCCCT CAACACTGTC CTGGTCGAGG
    GACGAGAGCA CCCGAAGGGC ATCCCGGCTG GAGAAGCAGA ATAGCACACC TGAGAGTGAC
    TACGACAACA CTCCCAACGA CAGGGACCTG GATGACATGG GGTCAAGCAG GAAAGGAAGG
    CAGAGGAGCT CGGTGTGGCA GAGTGACAGC TCAGGCCCAG ACACGGCAGA GCCCCATGTG
    GCCCCTAGCC CCATCCTCCC CAGCACCGAA GACGTCATCC GGAAGACAGA GCAGATCACC
    AAAAACATAC AGGAGCTCCT AAGGGCAGCC CAAGAAAATA AACATGACAG TTATATTCCC
    TGCTCAGAGA GGATACATGT GGCTGTTACA GAAATGGCAG CGTTATTCCC CAAAAAACCC
    AAGTCCGACA TGGTGAGGAC CTCCCTCCGC TTACTGACGT CCAGTGCCTA CCGACTGCAG
    TCAGAATGCA AGAAGACCCT CCCGGGGGAC CCGGGCCCGC CCACGGACAT CCAGCTGGTC
    ACGCAGCAGG TCATCCAGTG TGCCTACGAC ATCGCCAAGG CCGCCAAGCA GCTGGTCACC
    ATCACCACCA AAGAGAACAA CAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27880
    Clone ID Related Accession (Same CDS sequence) XM_005217838.3 , XM_005217838.4
    Accession Version XM_005217838.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217838.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217895.1
    CDS149..2188
    Translation

    Target ORF information:

    RefSeq Version XM_005217838.3
    Organism Bos taurus(cattle)
    Definition Bos taurus G protein-coupled receptor kinase interacting ArfGAP 2 (GIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217838.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27880
    Clone ID Related Accession (Same CDS sequence) XM_005217838.3 , XM_005217838.4
    Accession Version XM_005217838.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217838.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217895.1
    CDS148..2187
    Translation

    Target ORF information:

    RefSeq Version XM_005217838.4
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217838.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGCTTCAAAC ACTCCAGAGT
    GAAAATTCGA ACCTCAGGAA ACAGGCCACA ACCAATATAT ATCAGGTGCA AACTGGTTCT
    GAGTACACAG ACACTTCCAA CCACTCTTCC TTAAAGCGAC GTCCGTCTGC CCGGGGCAGT
    AGGCCCATGT CCATGTACGA GACAGGATCG GGTCAGAAAC CCTATCTCCC AATGGGAGAA
    GTGAACCATC CCGAAGAGAG CAGGACGAGA CTCCAGCCTT TCCCGGCGCA CGCATCCCGG
    CTGGAGAAGC AGAATAGCAC ACCTGAGAGT GACTACGACA ACACTCCCAA CGACAGGGAC
    CTGGATGACA TGGGGTCAAG CAGGAAAGGA AGGCAGAGGA GCTCGGTGTG GCAGAGTGAC
    AGCTCAGGCC CAGACACGGC AGAGCCCCAT GTGGCCCCTA GCCCCATCCT CCCCAGCACC
    GAAGACGTCA TCCGGAAGAC AGAGCAGATC ACCAAAAACA TACAGGAGCT CCTAAGGGCA
    GCCCAAGAAA ATAAACATGA CAGTTATATT CCCTGCTCAG AGAGGATACA TGTGGCTGTT
    ACAGAAATGG CAGCGTTATT CCCCAAAAAA CCCAAGTCCG ACATGGTGAG GACCTCCCTC
    CGCTTACTGA CGTCCAGTGC CTACCGACTG CAGTCAGAAT GCAAGAAGAC CCTCCCGGGG
    GACCCGGGCC CGCCCACGGA CATCCAGCTG GTCACGCAGC AGGTCATCCA GTGTGCCTAC
    GACATCGCCA AGGCCGCCAA GCAGCTGGTC ACCATCACCA CCAAAGAGAA CAACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224260
    Clone ID Related Accession (Same CDS sequence) XM_024977222.1
    Accession Version XM_024977222.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1896bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGCA TCCCGGCTGG AGAAGCAGAA TAGCACACCT
    GAGAGTGACT ACGACAACAC TCCCAACGAC AGGGACCTGG ATGACATGGG GTCAAGCAGG
    AAAGGAAGGC AGAGGAGCTC GGTGTGGCAG AGTGACAGCT CAGGCCCAGA CACGGCAGAG
    CCCCATGTGG CCCCTAGCCC CATCCTCCCC AGCACCGAAG ACGTCATCCG GAAGACAGAG
    CAGATCACCA AAAACATACA GGAGCTCCTA AGGGCAGCCC AAGAAAATAA ACATGACAGT
    TATATTCCCT GCTCAGAGAG GATACATGTG GCTGTTACAG AAATGGCAGC GTTATTCCCC
    AAAAAACCCA AGTCCGACAT GGTGAGGACC TCCCTCCGCT TACTGACGTC CAGTGCCTAC
    CGACTGCAGT CAGAATGCAA GAAGACCCTC CCGGGGGACC CGGGCCCGCC CACGGACATC
    CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCCTACGACA TCGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832990.1
    CDS147..2042
    Translation

    Target ORF information:

    RefSeq Version XM_024977222.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977222.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCAGCCT GGATTTGTCT
    GAATTGGCAA AAGCTGCTAA GAAGAAACTT CAGTCTCTAA GTAATCATTT GTTTGAAGAA
    CTTGCCATGG ATGTGTACGA TGAAGTTGAC AGGCGAGAGA CTGATGCAGT CTGGCTTGCC
    ACGCAGAACC ACAGCACCCT GGTGACCGAG ACAACCGTCG TCCCCTTCCT TCCAGTCAAT
    CCCGAGTACT CATCGACACG GAACCAGGGC AGACAGAAAC TAGCTCGGTT TAACGCCCAT
    GAGTTTGCCA CTCTGGTTAT CGACATTCTC AGTGATGCCA AGAGGAGACA GCAAGGCAGC
    CCTCTCTCTA GTTCAAAAGA CAATGTGGAA CTGATACTGA AAACAATTAG TAACCAGCAC
    AGTGTTGAGA GTCAGGACAA TGACCAGCCA GACTACGACA GTGTGGCGTC AGACGAAGAC
    ACGGATCCGG AAGCCACTGC AAGCAAGGCC CACAGGCAAA AGAGCCTAGA TTCAGATTTA
    TCAGATGGAC CAGTCACTGT GCAGGAATTT TTGGAGGTCA AAAACGCTCT AGTGGCTTCT
    GAGGCCAAGA TACAACAGCT AATGAAGGCA TCCCGGCTGG AGAAGCAGAA TAGCACACCT
    GAGAGTGACT ACGACAACAC TCCCAACGAC AGGGACCTGG ATGACATGGG GTCAAGCAGG
    AAAGGAAGGC AGAGGAGCTC GGTGTGGCAG AGTGACAGCT CAGGCCCAGA CACGGCAGAG
    CCCCATGTGG CCCCTAGCCC CATCCTCCCC AGCACCGAAG ACGTCATCCG GAAGACAGAG
    CAGATCACCA AAAACATACA GGAGCTCCTA AGGGCAGCCC AAGAAAATAA ACATGACAGT
    TATATTCCCT GCTCAGAGAG GATACATGTG GCTGTTACAG AAATGGCAGC GTTATTCCCC
    AAAAAACCCA AGTCCGACAT GGTGAGGACC TCCCTCCGCT TACTGACGTC CAGTGCCTAC
    CGACTGCAGT CAGAATGCAA GAAGACCCTC CCGGGGGACC CGGGCCCGCC CACGGACATC
    CAGCTGGTCA CGCAGCAGGT CATCCAGTGT GCCTACGACA TCGCCAAGGC CGCCAAGCAG
    CTGGTCACCA TCACCACCAA AGAGAACAAC AACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa224261
    Clone ID Related Accession (Same CDS sequence) XM_024977223.1
    Accession Version XM_024977223.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product ARF GTPase-activating protein GIT2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGG CATCCCGGCT GGAGAAGCAG
    AATAGCACAC CTGAGAGTGA CTACGACAAC ACTCCCAACG ACAGGGACCT GGATGACATG
    GGGTCAAGCA GGAAAGGAAG GCAGAGGAGC TCGGTGTGGC AGAGTGACAG CTCAGGCCCA
    GACACGGCAG AGCCCCATGT GGCCCCTAGC CCCATCCTCC CCAGCACCGA AGACGTCATC
    CGGAAGACAG AGCAGATCAC CAAAAACATA CAGGAGCTCC TAAGGGCAGC CCAAGAAAAT
    AAACATGACA GTTATATTCC CTGCTCAGAG AGGATACATG TGGCTGTTAC AGAAATGGCA
    GCGTTATTCC CCAAAAAACC CAAGTCCGAC ATGGTGAGGA CCTCCCTCCG CTTACTGACG
    TCCAGTGCCT ACCGACTGCA GTCAGAATGC AAGAAGACCC TCCCGGGGGA CCCGGGCCCG
    CCCACGGACA TCCAGCTGGT CACGCAGCAG GTCATCCAGT GTGCCTACGA CATCGCCAAG
    GCCGCCAAGC AGCTGGTCAC CATCACCACC AAAGAGAACA ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832991.1
    CDS147..1934
    Translation

    Target ORF information:

    RefSeq Version XM_024977223.1
    Organism Bos taurus(cattle)
    Definition Bos taurus GIT ArfGAP 2 (GIT2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977223.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTCGAAGC GGCTCCGGAG CAGCGAGGTG TGCGCTGACT GCAGCGGGCC GGATCCTTCC 
    TGGGCATCCG TAAATAGGGG AACGTTTATA TGCGATGAGT GCTGCAGTGT CCATCGGAGT
    CTGGGACGCC ATATCTCTCA AGTGAGGCAT CTTAAACACA CACCATGGCC TCCGACATTG
    CTTCAGATGG TTGAAACCTT GTATAATAAT GGTGCTAATT CTATATGGGA GCATTCGTTA
    CTGGACCCCA CCTCTATTAT GAGTGGAAGA CGCAAAGCTA ATCCACAGGA TAAAGTACAT
    CCTAATAAAG CAGAATTCAT CAGAGCCAAG TATCAGATGT TAGCGTTCGT CCACCGCTTG
    CCCTGCCGGG ATGACGACAG TGTGACCGCC AAAGATCTCA GTAAGCAACT CCATTCAAGC
    GTGAGAACAG GGAATCTTGA AACCTGTTTG AGGCTGTTAT CTTTAGGAGC ACAAGCCAAC
    TTTTTTCACC CTGAGAAAGG AAACACCCCC CTCCATGTGG CCTCCAAAGC AGGGCAGATT
    CTACAGGCAG AATTGTTGGC AGTATACGGA GCAGACCCAG GCACCCAGGA CTCCAGTGGG
    AAAACTCCTG TCGACTATGC CAGGCAAGGA GGGCACCACG AGCTGGCAGA GCGCCTTGTA
    GAAATACAGT ACGAGCTGAC TGACAGACTA GCCTTCTATC TCTGCGGCAG GAAACCAGAT
    CACAAAAATG GACAGCACTT TATAATACCT CAAATGGCGG ACAGCCTGGA TTTGTCTGAA
    TTGGCAAAAG CTGCTAAGAA GAAACTTCAG TCTCTAAGTA ATCATTTGTT TGAAGAACTT
    GCCATGGATG TGTACGATGA AGTTGACAGG CGAGAGACTG ATGCAGTCTG GCTTGCCACG
    CAGAACCACA GCACCCTGGT GACCGAGACA ACCGTCGTCC CCTTCCTTCC AGTCAATCCC
    GAGTACTCAT CGACACGGAA CCAGGGCAGA CAGAAACTAG CTCGGTTTAA CGCCCATGAG
    TTTGCCACTC TGGTTATCGA CATTCTCAGT GATGCCAAGA GGAGACAGCA AGGCAGCCCT
    CTCTCTAGTT CAAAAGACAA TGTGGAACTG ATACTGAAAA CAATTAGTAA CCAGCACAGT
    GTTGAGAGTC AGGACAATGA CCAGCCAGAC TACGACAGTG TGGCGTCAGA CGAAGACACG
    GATCCGGAAG CCACTGCAAG CAAGGCCCAC AGGCAAAAGG CATCCCGGCT GGAGAAGCAG
    AATAGCACAC CTGAGAGTGA CTACGACAAC ACTCCCAACG ACAGGGACCT GGATGACATG
    GGGTCAAGCA GGAAAGGAAG GCAGAGGAGC TCGGTGTGGC AGAGTGACAG CTCAGGCCCA
    GACACGGCAG AGCCCCATGT GGCCCCTAGC CCCATCCTCC CCAGCACCGA AGACGTCATC
    CGGAAGACAG AGCAGATCAC CAAAAACATA CAGGAGCTCC TAAGGGCAGC CCAAGAAAAT
    AAACATGACA GTTATATTCC CTGCTCAGAG AGGATACATG TGGCTGTTAC AGAAATGGCA
    GCGTTATTCC CCAAAAAACC CAAGTCCGAC ATGGTGAGGA CCTCCCTCCG CTTACTGACG
    TCCAGTGCCT ACCGACTGCA GTCAGAATGC AAGAAGACCC TCCCGGGGGA CCCGGGCCCG
    CCCACGGACA TCCAGCTGGT CACGCAGCAG GTCATCCAGT GTGCCTACGA CATCGCCAAG
    GCCGCCAAGC AGCTGGTCAC CATCACCACC AAAGAGAACA ACAACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.