E4F1 cDNA ORF clone, Bos taurus(cattle)

The following E4F1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the E4F1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216219 NM_001205825.2
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa40813 NM_001205825.1
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32738 XM_005224451.4
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32739 XM_005224452.4
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32740 XM_005224453.4
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32741 XM_005224454.4
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa49489 XM_010819096.3
Latest version!
Bos taurus E4F transcription factor 1 (E4F1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
View Old Accession Versions >>
OBa32740 XM_005224453.3 Bos taurus E4F transcription factor 1 (E4F1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32741 XM_005224454.3 Bos taurus E4F transcription factor 1 (E4F1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32738 XM_005224451.3 Bos taurus E4F transcription factor 1 (E4F1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa32739 XM_005224452.3 Bos taurus E4F transcription factor 1 (E4F1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa49489 XM_010819096.2 Bos taurus E4F transcription factor 1 (E4F1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216219
    Clone ID Related Accession (Same CDS sequence) NM_001205825.2
    Accession Version NM_001205825.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product transcription factor E4F1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Jun 30, 2017 this sequence version replaced NM_001205825.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145415, SAMN03145416 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGCAG
    GTGGCCGGCG AGGCCTCGTC TGTGCCCAGG ACCCACCCGT GCCCTCAGTG CAGTGAGACC
    TTCCCAACGG CGGCCACCCT GGAGGCCCAC AAAAGAGGCC ATGCAGGGCC GAGGCCCTTC
    ACGTGCCCGC AGTGTGGCAA GGCCTTCCCC AAGGCCTACC TGCTCAAGAA GCACCAGGAG
    GTGCACGTGC ACGAGCGCCG CTTCCGCTGC GGCGACTGTG GGAAGCTCTA CAAGACCATC
    GCCCACGTCC GCGGCCACCG GCGCGTCCAC TCGGACGAGC GGCCCTACCC CTGTCCTGAG
    TGCGGCAAGT GCTACAAGAC CAAGAACGCC CAGCAGGTGC ACTTCCGGAC GCACCTAGAG
    GAGAAGCCGC ATGTGTGCCC GTTCTGCAGC CGCGGCTTCC GGGAGAAGGG CTCGCTGGTG
    CGGCACGTGC GGCACCACAC GGGCGAGAAG CCCTTCAAGT GCTACAAGTG TGGCCGCGGC
    TTCGCCGAGC ACGGCACACT CAACCGGCAC CTGCGGACCA AAGGGGGCTG CCTGTTGGAG
    GTGGAGGAGC TGCTGGTGTC TGAAGAGAGC CCCACTGCGG CCGCCGCCGT CCTGACCGAG
    GACCCACACA CCGTGTTGGT CGAGTTCTCG TCTGTGGTAG CCGACACGCA GGAGTACATC
    ATAGAGGCCA CTGCAGATGA TGCAGAGACC AGCGAAGCCA CGGAGATCAT CGAGGGCACC
    CAGACGGAGG TGGACAGTCA CATCATGAAG GTGGTGCAGC AGATAGTGCA CCAGGCCAGC
    GCTGGGCACC AGATCATCGT GCAGAACGTG ACCATGGACC AGGAGGCGCG GCTGGGCCCA
    GAGGCGGCTG CAGCCGACAC CATCACCATC GCCACCCCCG AGAGCCTGAC GGAGCAGGTG
    GCCATGACGC TGGCCTCGGC CATCAGCGAG GGCACTGTGC TTACGGCCCG CTCGGGCACC
    GGTGGCGCTG AGCAGGCCAC TGTGACCATG GTTTCGTCGG AGGACATTGA GATCCTGGAG
    CATGCTGGCG AGCTAGTTAT CGCCTCCCCG GAGGGCCAGC TTGAGGTGCA GACGGTCATC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192754.1
    CDS316..2661
    Translation

    Target ORF information:

    RefSeq Version NM_001205825.2
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205825.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGCAG
    GTGGCCGGCG AGGCCTCGTC TGTGCCCAGG ACCCACCCGT GCCCTCAGTG CAGTGAGACC
    TTCCCAACGG CGGCCACCCT GGAGGCCCAC AAAAGAGGCC ATGCAGGGCC GAGGCCCTTC
    ACGTGCCCGC AGTGTGGCAA GGCCTTCCCC AAGGCCTACC TGCTCAAGAA GCACCAGGAG
    GTGCACGTGC ACGAGCGCCG CTTCCGCTGC GGCGACTGTG GGAAGCTCTA CAAGACCATC
    GCCCACGTCC GCGGCCACCG GCGCGTCCAC TCGGACGAGC GGCCCTACCC CTGTCCTGAG
    TGCGGCAAGT GCTACAAGAC CAAGAACGCC CAGCAGGTGC ACTTCCGGAC GCACCTAGAG
    GAGAAGCCGC ATGTGTGCCC GTTCTGCAGC CGCGGCTTCC GGGAGAAGGG CTCGCTGGTG
    CGGCACGTGC GGCACCACAC GGGCGAGAAG CCCTTCAAGT GCTACAAGTG TGGCCGCGGC
    TTCGCCGAGC ACGGCACACT CAACCGGCAC CTGCGGACCA AAGGGGGCTG CCTGTTGGAG
    GTGGAGGAGC TGCTGGTGTC TGAAGAGAGC CCCACTGCGG CCGCCGCCGT CCTGACCGAG
    GACCCACACA CCGTGTTGGT CGAGTTCTCG TCTGTGGTAG CCGACACGCA GGAGTACATC
    ATAGAGGCCA CTGCAGATGA TGCAGAGACC AGCGAAGCCA CGGAGATCAT CGAGGGCACC
    CAGACGGAGG TGGACAGTCA CATCATGAAG GTGGTGCAGC AGATAGTGCA CCAGGCCAGC
    GCTGGGCACC AGATCATCGT GCAGAACGTG ACCATGGACC AGGAGGCGCG GCTGGGCCCA
    GAGGCGGCTG CAGCCGACAC CATCACCATC GCCACCCCCG AGAGCCTGAC GGAGCAGGTG
    GCCATGACGC TGGCCTCGGC CATCAGCGAG GGCACTGTGC TTACGGCCCG CTCGGGCACC
    GGTGGCGCTG AGCAGGCCAC TGTGACCATG GTTTCGTCGG AGGACATTGA GATCCTGGAG
    CATGCTGGCG AGCTAGTTAT CGCCTCCCCG GAGGGCCAGC TTGAGGTGCA GACGGTCATC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40813
    Clone ID Related Accession (Same CDS sequence) NM_001205825.1
    Accession Version NM_001205825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product transcription factor E4F1
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03056638.1. On Apr 20, 2011 this sequence version replaced XM_594119.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01093735, SAMN01093745 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CGGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGCAG
    GTGGCCGGCG AGGCCTCGTC TGTGCCCAGG ACCCACCCGT GCCCTCAGTG CAGTGAGACC
    TTCCCAACGG CGGCCACCCT GGAGGCCCAC AAAAGAGGCC ATGCAGGGCC GAGGCCCTTC
    ACGTGCCCGC AGTGTGGCAA GGCCTTCCCC AAGGCCTACC TGCTCAAGAA GCACCAGGAG
    GTGCACGTGC ACGAGCGCCG CTTCCGCTGC GGCGACTGTG GGAAGCTCTA CAAGACCATC
    GCCCACGTCC GCGGCCACCG GCGCGTCCAC TCGGACGAGC GGCCCTACCC CTGTCCTGAG
    TGCGGCAAGT GCTACAAGAC CAAGAACGCC CAGCAGGTGC ACTTCCGGAC GCACCTAGAG
    GAGAAGCCGC ATGTGTGCCC GTTCTGCAGC CGCGGCTTCC GGGAGAAGGG CTCGCTGGTG
    CGGCACGTGC GGCACCACAC GGGCGAGAAG CCCTTCAAGT GCTACAAGTG TGGCCGCGGC
    TTCGCCGAGC ACGGCACACT CAACCGGCAC CTGCGGACCA AAGGGGGCTG CCTGTTGGAG
    GTGGAGGAGC TGCTGGTGTC TGAAGAGAGC CCCACTGCGG CCGCCGCCGT CCTGACCGAG
    GACCCACACA CCGTGTTGGT CGAGTTCTCG TCTGTGGTAG CCGACACGCA GGAGTACATC
    ATAGAGGCCA CTGCAGATGA TGCAGAGACC AGCGAAGCCA CGGAGATCAT CGAGGGCACC
    CAGACGGAGG TGGACAGTCA CATCATGAAG GTGGTGCAGC AGATAGTGCA CCAGGCCAGC
    GCTGGGCACC AGATCATCGT GCAGAACGTG ACCATGGACC AGGAGGCGCG GCTGGGCCCA
    GAGGCGGCTG CAGCCGACAC CATCACCATC GCCACCCCCG AGAGCCTGAC GGAGCAGGTG
    GCCATGACGC TGGCCTCGGC CATCAGCGAG GGCACTGTGC TTACGGCCCG CTCGGGCACC
    GGTGGCGCTG AGCAGGCCAC TGTGACCATG GTTTCGTCGG AGGACATTGA GATCCTGGAG
    CATGCTGGCG AGCTAGTTAT CGCCTCCCCG GAGGGCCAGC TTGAGGTGCA GACGGTCATC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192754.1
    CDS316..2661
    Translation

    Target ORF information:

    RefSeq Version NM_001205825.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CGGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGCAG
    GTGGCCGGCG AGGCCTCGTC TGTGCCCAGG ACCCACCCGT GCCCTCAGTG CAGTGAGACC
    TTCCCAACGG CGGCCACCCT GGAGGCCCAC AAAAGAGGCC ATGCAGGGCC GAGGCCCTTC
    ACGTGCCCGC AGTGTGGCAA GGCCTTCCCC AAGGCCTACC TGCTCAAGAA GCACCAGGAG
    GTGCACGTGC ACGAGCGCCG CTTCCGCTGC GGCGACTGTG GGAAGCTCTA CAAGACCATC
    GCCCACGTCC GCGGCCACCG GCGCGTCCAC TCGGACGAGC GGCCCTACCC CTGTCCTGAG
    TGCGGCAAGT GCTACAAGAC CAAGAACGCC CAGCAGGTGC ACTTCCGGAC GCACCTAGAG
    GAGAAGCCGC ATGTGTGCCC GTTCTGCAGC CGCGGCTTCC GGGAGAAGGG CTCGCTGGTG
    CGGCACGTGC GGCACCACAC GGGCGAGAAG CCCTTCAAGT GCTACAAGTG TGGCCGCGGC
    TTCGCCGAGC ACGGCACACT CAACCGGCAC CTGCGGACCA AAGGGGGCTG CCTGTTGGAG
    GTGGAGGAGC TGCTGGTGTC TGAAGAGAGC CCCACTGCGG CCGCCGCCGT CCTGACCGAG
    GACCCACACA CCGTGTTGGT CGAGTTCTCG TCTGTGGTAG CCGACACGCA GGAGTACATC
    ATAGAGGCCA CTGCAGATGA TGCAGAGACC AGCGAAGCCA CGGAGATCAT CGAGGGCACC
    CAGACGGAGG TGGACAGTCA CATCATGAAG GTGGTGCAGC AGATAGTGCA CCAGGCCAGC
    GCTGGGCACC AGATCATCGT GCAGAACGTG ACCATGGACC AGGAGGCGCG GCTGGGCCCA
    GAGGCGGCTG CAGCCGACAC CATCACCATC GCCACCCCCG AGAGCCTGAC GGAGCAGGTG
    GCCATGACGC TGGCCTCGGC CATCAGCGAG GGCACTGTGC TTACGGCCCG CTCGGGCACC
    GGTGGCGCTG AGCAGGCCAC TGTGACCATG GTTTCGTCGG AGGACATTGA GATCCTGGAG
    CATGCTGGCG AGCTAGTTAT CGCCTCCCCG GAGGGCCAGC TTGAGGTGCA GACGGTCATC
    GTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32738
    Clone ID Related Accession (Same CDS sequence) XM_005224451.3 , XM_005224451.4
    Accession Version XM_005224451.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGCAGGTGGC CGGCGAGGCC TCGTCTGTGC CCAGGACCCA CCCGTGCCCT
    CAGTGCAGTG AGACCTTCCC AACGGCGGCC ACCCTGGAGG CCCACAAAAG AGGCCATGCA
    GGGCCGAGGC CCTTCACGTG CCCGCAGTGT GGCAAGGCCT TCCCCAAGGC CTACCTGCTC
    AAGAAGCACC AGGAGGTGCA CGTGCACGAG CGCCGCTTCC GCTGCGGCGA CTGTGGGAAG
    CTCTACAAGA CCATCGCCCA CGTCCGCGGC CACCGGCGCG TCCACTCGGA CGAGCGGCCC
    TACCCCTGTC CTGAGTGCGG CAAGTGCTAC AAGACCAAGA ACGCCCAGCA GGTGCACTTC
    CGGACGCACC TAGAGGAGAA GCCGCATGTG TGCCCGTTCT GCAGCCGCGG CTTCCGGGAG
    AAGGGCTCGC TGGTGCGGCA CGTGCGGCAC CACACGGGCG AGAAGCCCTT CAAGTGCTAC
    AAGTGTGGCC GCGGCTTCGC CGAGCACGGC ACACTCAACC GGCACCTGCG GACCAAAGGG
    GGCTGCCTGT TGGAGGTGGA GGAGCTGCTG GTGTCTGAAG AGAGCCCCAC TGCGGCCGCC
    GCCGTCCTGA CCGAGGACCC ACACACCGTG TTGGTCGAGT TCTCGTCTGT GGTAGCCGAC
    ACGCAGGAGT ACATCATAGA GGCCACTGCA GATGATGCAG AGACCAGCGA AGCCACGGAG
    ATCATCGAGG GCACCCAGAC GGAGGTGGAC AGTCACATCA TGAAGGTGGT GCAGCAGATA
    GTGCACCAGG CCAGCGCTGG GCACCAGATC ATCGTGCAGA ACGTGACCAT GGACCAGGAG
    GCGCGGCTGG GCCCAGAGGC GGCTGCAGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACGGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTACG
    GCCCGCTCGG GCACCGGTGG CGCTGAGCAG GCCACTGTGA CCATGGTTTC GTCGGAGGAC
    ATTGAGATCC TGGAGCATGC TGGCGAGCTA GTTATCGCCT CCCCGGAGGG CCAGCTTGAG
    GTGCAGACGG TCATCGTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224508.1
    CDS310..2730
    Translation

    Target ORF information:

    RefSeq Version XM_005224451.3
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGCAGGTGGC CGGCGAGGCC TCGTCTGTGC CCAGGACCCA CCCGTGCCCT
    CAGTGCAGTG AGACCTTCCC AACGGCGGCC ACCCTGGAGG CCCACAAAAG AGGCCATGCA
    GGGCCGAGGC CCTTCACGTG CCCGCAGTGT GGCAAGGCCT TCCCCAAGGC CTACCTGCTC
    AAGAAGCACC AGGAGGTGCA CGTGCACGAG CGCCGCTTCC GCTGCGGCGA CTGTGGGAAG
    CTCTACAAGA CCATCGCCCA CGTCCGCGGC CACCGGCGCG TCCACTCGGA CGAGCGGCCC
    TACCCCTGTC CTGAGTGCGG CAAGTGCTAC AAGACCAAGA ACGCCCAGCA GGTGCACTTC
    CGGACGCACC TAGAGGAGAA GCCGCATGTG TGCCCGTTCT GCAGCCGCGG CTTCCGGGAG
    AAGGGCTCGC TGGTGCGGCA CGTGCGGCAC CACACGGGCG AGAAGCCCTT CAAGTGCTAC
    AAGTGTGGCC GCGGCTTCGC CGAGCACGGC ACACTCAACC GGCACCTGCG GACCAAAGGG
    GGCTGCCTGT TGGAGGTGGA GGAGCTGCTG GTGTCTGAAG AGAGCCCCAC TGCGGCCGCC
    GCCGTCCTGA CCGAGGACCC ACACACCGTG TTGGTCGAGT TCTCGTCTGT GGTAGCCGAC
    ACGCAGGAGT ACATCATAGA GGCCACTGCA GATGATGCAG AGACCAGCGA AGCCACGGAG
    ATCATCGAGG GCACCCAGAC GGAGGTGGAC AGTCACATCA TGAAGGTGGT GCAGCAGATA
    GTGCACCAGG CCAGCGCTGG GCACCAGATC ATCGTGCAGA ACGTGACCAT GGACCAGGAG
    GCGCGGCTGG GCCCAGAGGC GGCTGCAGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACGGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTACG
    GCCCGCTCGG GCACCGGTGG CGCTGAGCAG GCCACTGTGA CCATGGTTTC GTCGGAGGAC
    ATTGAGATCC TGGAGCATGC TGGCGAGCTA GTTATCGCCT CCCCGGAGGG CCAGCTTGAG
    GTGCAGACGG TCATCGTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32738
    Clone ID Related Accession (Same CDS sequence) XM_005224451.3 , XM_005224451.4
    Accession Version XM_005224451.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224451.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGCAGGTGGC CGGCGAGGCC TCGTCTGTGC CCAGGACCCA CCCGTGCCCT
    CAGTGCAGTG AGACCTTCCC AACGGCGGCC ACCCTGGAGG CCCACAAAAG AGGCCATGCA
    GGGCCGAGGC CCTTCACGTG CCCGCAGTGT GGCAAGGCCT TCCCCAAGGC CTACCTGCTC
    AAGAAGCACC AGGAGGTGCA CGTGCACGAG CGCCGCTTCC GCTGCGGCGA CTGTGGGAAG
    CTCTACAAGA CCATCGCCCA CGTCCGCGGC CACCGGCGCG TCCACTCGGA CGAGCGGCCC
    TACCCCTGTC CTGAGTGCGG CAAGTGCTAC AAGACCAAGA ACGCCCAGCA GGTGCACTTC
    CGGACGCACC TAGAGGAGAA GCCGCATGTG TGCCCGTTCT GCAGCCGCGG CTTCCGGGAG
    AAGGGCTCGC TGGTGCGGCA CGTGCGGCAC CACACGGGCG AGAAGCCCTT CAAGTGCTAC
    AAGTGTGGCC GCGGCTTCGC CGAGCACGGC ACACTCAACC GGCACCTGCG GACCAAAGGG
    GGCTGCCTGT TGGAGGTGGA GGAGCTGCTG GTGTCTGAAG AGAGCCCCAC TGCGGCCGCC
    GCCGTCCTGA CCGAGGACCC ACACACCGTG TTGGTCGAGT TCTCGTCTGT GGTAGCCGAC
    ACGCAGGAGT ACATCATAGA GGCCACTGCA GATGATGCAG AGACCAGCGA AGCCACGGAG
    ATCATCGAGG GCACCCAGAC GGAGGTGGAC AGTCACATCA TGAAGGTGGT GCAGCAGATA
    GTGCACCAGG CCAGCGCTGG GCACCAGATC ATCGTGCAGA ACGTGACCAT GGACCAGGAG
    GCGCGGCTGG GCCCAGAGGC GGCTGCAGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACGGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTACG
    GCCCGCTCGG GCACCGGTGG CGCTGAGCAG GCCACTGTGA CCATGGTTTC GTCGGAGGAC
    ATTGAGATCC TGGAGCATGC TGGCGAGCTA GTTATCGCCT CCCCGGAGGG CCAGCTTGAG
    GTGCAGACGG TCATCGTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224508.1
    CDS365..2785
    Translation

    Target ORF information:

    RefSeq Version XM_005224451.4
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224451.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGCAGGTGGC CGGCGAGGCC TCGTCTGTGC CCAGGACCCA CCCGTGCCCT
    CAGTGCAGTG AGACCTTCCC AACGGCGGCC ACCCTGGAGG CCCACAAAAG AGGCCATGCA
    GGGCCGAGGC CCTTCACGTG CCCGCAGTGT GGCAAGGCCT TCCCCAAGGC CTACCTGCTC
    AAGAAGCACC AGGAGGTGCA CGTGCACGAG CGCCGCTTCC GCTGCGGCGA CTGTGGGAAG
    CTCTACAAGA CCATCGCCCA CGTCCGCGGC CACCGGCGCG TCCACTCGGA CGAGCGGCCC
    TACCCCTGTC CTGAGTGCGG CAAGTGCTAC AAGACCAAGA ACGCCCAGCA GGTGCACTTC
    CGGACGCACC TAGAGGAGAA GCCGCATGTG TGCCCGTTCT GCAGCCGCGG CTTCCGGGAG
    AAGGGCTCGC TGGTGCGGCA CGTGCGGCAC CACACGGGCG AGAAGCCCTT CAAGTGCTAC
    AAGTGTGGCC GCGGCTTCGC CGAGCACGGC ACACTCAACC GGCACCTGCG GACCAAAGGG
    GGCTGCCTGT TGGAGGTGGA GGAGCTGCTG GTGTCTGAAG AGAGCCCCAC TGCGGCCGCC
    GCCGTCCTGA CCGAGGACCC ACACACCGTG TTGGTCGAGT TCTCGTCTGT GGTAGCCGAC
    ACGCAGGAGT ACATCATAGA GGCCACTGCA GATGATGCAG AGACCAGCGA AGCCACGGAG
    ATCATCGAGG GCACCCAGAC GGAGGTGGAC AGTCACATCA TGAAGGTGGT GCAGCAGATA
    GTGCACCAGG CCAGCGCTGG GCACCAGATC ATCGTGCAGA ACGTGACCAT GGACCAGGAG
    GCGCGGCTGG GCCCAGAGGC GGCTGCAGCC GACACCATCA CCATCGCCAC CCCCGAGAGC
    CTGACGGAGC AGGTGGCCAT GACGCTGGCC TCGGCCATCA GCGAGGGCAC TGTGCTTACG
    GCCCGCTCGG GCACCGGTGG CGCTGAGCAG GCCACTGTGA CCATGGTTTC GTCGGAGGAC
    ATTGAGATCC TGGAGCATGC TGGCGAGCTA GTTATCGCCT CCCCGGAGGG CCAGCTTGAG
    GTGCAGACGG TCATCGTCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32739
    Clone ID Related Accession (Same CDS sequence) XM_005224452.4 , XM_005224452.3
    Accession Version XM_005224452.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224452.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224509.1
    CDS366..2783
    Translation

    Target ORF information:

    RefSeq Version XM_005224452.4
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224452.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32739
    Clone ID Related Accession (Same CDS sequence) XM_005224452.4 , XM_005224452.3
    Accession Version XM_005224452.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224452.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224509.1
    CDS310..2727
    Translation

    Target ORF information:

    RefSeq Version XM_005224452.3
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224452.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGAGGCC
    TGGAGTTCCT CTGGGCTACC CCGTGGATTC GGAGCATCCC TTCCCGCCAT CCCTTCTGGC
    CGAGCAGGAG GCGGACACAT CAAAGAGGTC ATCGTGGCTT CAGAGGCAGA GCCGGGGGAC
    AGCGAGATGG CCGAGGGCCC AGGCAGCTCC AGTGAGCGGG GGCCCAGGCT CGCTGCAGAG
    GGCGAGCAGG CCCAGGTCAA ACTGCTGGTG AACAAGGACG GCCGCTACGT GTGCATGCTC
    TGCCACAAGA CCTTCAAGAC GGGCAGCATC CTCAAGGCCC ACATGGTCAC GCACAGCAGC
    CGCAAGGACC ACGAGTGCAA ACTGTGTGGG GCCTCTTTCC GGACCAAAGG CTCACTCATC
    CGGCACCACC GGAGGCACAC AGACGAGCGC CCCTATAAGT GTGCCAAGTG TGGGAAGAGC
    TTCCGGGAGT CGGGGGCACT GACCCGGCAC CTGAAGTCTC TCACCCCGTG TACAGAGAAA
    ATCCGCTTCA GCATGAGCAA AGACGTGGTC GTCGGCAAAG AGGACGTGCC TGCAGGGTCC
    GGTGCCTCCA CTGTGGGGCC TGTCACCCCG TCGTCGGCAG GTGAGCCCAT GGAGACGTCG
    CCGGTGATTC ACCTGGTGAC AGATGCCAAG GGCACCGTCA TCCATGAAGT CCACGTCCAG
    ATGCAAGAGC TTCCCCTGGG CATGAAGGCC CTCGCCCCGG AGCCCACCAG CCCCCAGGAG
    CTTCCCTGCT CCAGCGAGGG TGGCCGTGAG AACCTTCTGC ACCAAGCCAT GCAGAACTCC
    GGCATTGTCC TCGAGCGGGC CCCCGGGGAG GAGGGGGCCC TGGGGCCAGC CACCCCCACC
    GCGTCCAGTC CCCAGTCGCC AGGAGACGCT GCCCCGGAAC TGCCGCTGCT GGAGGTGGAG
    CAGGCGGAGA CGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32740
    Clone ID Related Accession (Same CDS sequence) XM_005224453.3 , XM_005224453.4
    Accession Version XM_005224453.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224453.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGGAGGC
    GGACACATCA AAGAGGTCAT CGTGGCTTCA GAGGCAGAGC CGGGGGACAG CGAGATGGCC
    GAGGGCCCAG GCAGCTCCAG TGAGCGGGGG CCCAGGCTCG CTGCAGAGGG CGAGCAGGCC
    CAGGTCAAAC TGCTGGTGAA CAAGGACGGC CGCTACGTGT GCATGCTCTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACGC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAAC TGTGTGGGGC CTCTTTCCGG ACCAAAGGCT CACTCATCCG GCACCACCGG
    AGGCACACAG ACGAGCGCCC CTATAAGTGT GCCAAGTGTG GGAAGAGCTT CCGGGAGTCG
    GGGGCACTGA CCCGGCACCT GAAGTCTCTC ACCCCGTGTA CAGAGAAAAT CCGCTTCAGC
    ATGAGCAAAG ACGTGGTCGT CGGCAAAGAG GACGTGCCTG CAGGGTCCGG TGCCTCCACT
    GTGGGGCCTG TCACCCCGTC GTCGGCAGGT GAGCCCATGG AGACGTCGCC GGTGATTCAC
    CTGGTGACAG ATGCCAAGGG CACCGTCATC CATGAAGTCC ACGTCCAGAT GCAAGAGCTT
    CCCCTGGGCA TGAAGGCCCT CGCCCCGGAG CCCACCAGCC CCCAGGAGCT TCCCTGCTCC
    AGCGAGGGTG GCCGTGAGAA CCTTCTGCAC CAAGCCATGC AGAACTCCGG CATTGTCCTC
    GAGCGGGCCC CCGGGGAGGA GGGGGCCCTG GGGCCAGCCA CCCCCACCGC GTCCAGTCCC
    CAGTCGCCAG GAGACGCTGC CCCGGAACTG CCGCTGCTGG AGGTGGAGCA GGCGGAGACG
    CAGGTGGCCG GCGAGGCCTC GTCTGTGCCC AGGACCCACC CGTGCCCTCA GTGCAGTGAG
    ACCTTCCCAA CGGCGGCCAC CCTGGAGGCC CACAAAAGAG GCCATGCAGG GCCGAGGCCC
    TTCACGTGCC CGCAGTGTGG CAAGGCCTTC CCCAAGGCCT ACCTGCTCAA GAAGCACCAG
    GAGGTGCACG TGCACGAGCG CCGCTTCCGC TGCGGCGACT GTGGGAAGCT CTACAAGACC
    ATCGCCCACG TCCGCGGCCA CCGGCGCGTC CACTCGGACG AGCGGCCCTA CCCCTGTCCT
    GAGTGCGGCA AGTGCTACAA GACCAAGAAC GCCCAGCAGG TGCACTTCCG GACGCACCTA
    GAGGAGAAGC CGCATGTGTG CCCGTTCTGC AGCCGCGGCT TCCGGGAGAA GGGCTCGCTG
    GTGCGGCACG TGCGGCACCA CACGGGCGAG AAGCCCTTCA AGTGCTACAA GTGTGGCCGC
    GGCTTCGCCG AGCACGGCAC ACTCAACCGG CACCTGCGGA CCAAAGGGGG CTGCCTGTTG
    GAGGTGGAGG AGCTGCTGGT GTCTGAAGAG AGCCCCACTG CGGCCGCCGC CGTCCTGACC
    GAGGACCCAC ACACCGTGTT GGTCGAGTTC TCGTCTGTGG TAGCCGACAC GCAGGAGTAC
    ATCATAGAGG CCACTGCAGA TGATGCAGAG ACCAGCGAAG CCACGGAGAT CATCGAGGGC
    ACCCAGACGG AGGTGGACAG TCACATCATG AAGGTGGTGC AGCAGATAGT GCACCAGGCC
    AGCGCTGGGC ACCAGATCAT CGTGCAGAAC GTGACCATGG ACCAGGAGGC GCGGCTGGGC
    CCAGAGGCGG CTGCAGCCGA CACCATCACC ATCGCCACCC CCGAGAGCCT GACGGAGCAG
    GTGGCCATGA CGCTGGCCTC GGCCATCAGC GAGGGCACTG TGCTTACGGC CCGCTCGGGC
    ACCGGTGGCG CTGAGCAGGC CACTGTGACC ATGGTTTCGT CGGAGGACAT TGAGATCCTG
    GAGCATGCTG GCGAGCTAGT TATCGCCTCC CCGGAGGGCC AGCTTGAGGT GCAGACGGTC
    ATCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224510.1
    CDS310..2658
    Translation

    Target ORF information:

    RefSeq Version XM_005224453.3
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224453.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGGAGGC
    GGACACATCA AAGAGGTCAT CGTGGCTTCA GAGGCAGAGC CGGGGGACAG CGAGATGGCC
    GAGGGCCCAG GCAGCTCCAG TGAGCGGGGG CCCAGGCTCG CTGCAGAGGG CGAGCAGGCC
    CAGGTCAAAC TGCTGGTGAA CAAGGACGGC CGCTACGTGT GCATGCTCTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACGC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAAC TGTGTGGGGC CTCTTTCCGG ACCAAAGGCT CACTCATCCG GCACCACCGG
    AGGCACACAG ACGAGCGCCC CTATAAGTGT GCCAAGTGTG GGAAGAGCTT CCGGGAGTCG
    GGGGCACTGA CCCGGCACCT GAAGTCTCTC ACCCCGTGTA CAGAGAAAAT CCGCTTCAGC
    ATGAGCAAAG ACGTGGTCGT CGGCAAAGAG GACGTGCCTG CAGGGTCCGG TGCCTCCACT
    GTGGGGCCTG TCACCCCGTC GTCGGCAGGT GAGCCCATGG AGACGTCGCC GGTGATTCAC
    CTGGTGACAG ATGCCAAGGG CACCGTCATC CATGAAGTCC ACGTCCAGAT GCAAGAGCTT
    CCCCTGGGCA TGAAGGCCCT CGCCCCGGAG CCCACCAGCC CCCAGGAGCT TCCCTGCTCC
    AGCGAGGGTG GCCGTGAGAA CCTTCTGCAC CAAGCCATGC AGAACTCCGG CATTGTCCTC
    GAGCGGGCCC CCGGGGAGGA GGGGGCCCTG GGGCCAGCCA CCCCCACCGC GTCCAGTCCC
    CAGTCGCCAG GAGACGCTGC CCCGGAACTG CCGCTGCTGG AGGTGGAGCA GGCGGAGACG
    CAGGTGGCCG GCGAGGCCTC GTCTGTGCCC AGGACCCACC CGTGCCCTCA GTGCAGTGAG
    ACCTTCCCAA CGGCGGCCAC CCTGGAGGCC CACAAAAGAG GCCATGCAGG GCCGAGGCCC
    TTCACGTGCC CGCAGTGTGG CAAGGCCTTC CCCAAGGCCT ACCTGCTCAA GAAGCACCAG
    GAGGTGCACG TGCACGAGCG CCGCTTCCGC TGCGGCGACT GTGGGAAGCT CTACAAGACC
    ATCGCCCACG TCCGCGGCCA CCGGCGCGTC CACTCGGACG AGCGGCCCTA CCCCTGTCCT
    GAGTGCGGCA AGTGCTACAA GACCAAGAAC GCCCAGCAGG TGCACTTCCG GACGCACCTA
    GAGGAGAAGC CGCATGTGTG CCCGTTCTGC AGCCGCGGCT TCCGGGAGAA GGGCTCGCTG
    GTGCGGCACG TGCGGCACCA CACGGGCGAG AAGCCCTTCA AGTGCTACAA GTGTGGCCGC
    GGCTTCGCCG AGCACGGCAC ACTCAACCGG CACCTGCGGA CCAAAGGGGG CTGCCTGTTG
    GAGGTGGAGG AGCTGCTGGT GTCTGAAGAG AGCCCCACTG CGGCCGCCGC CGTCCTGACC
    GAGGACCCAC ACACCGTGTT GGTCGAGTTC TCGTCTGTGG TAGCCGACAC GCAGGAGTAC
    ATCATAGAGG CCACTGCAGA TGATGCAGAG ACCAGCGAAG CCACGGAGAT CATCGAGGGC
    ACCCAGACGG AGGTGGACAG TCACATCATG AAGGTGGTGC AGCAGATAGT GCACCAGGCC
    AGCGCTGGGC ACCAGATCAT CGTGCAGAAC GTGACCATGG ACCAGGAGGC GCGGCTGGGC
    CCAGAGGCGG CTGCAGCCGA CACCATCACC ATCGCCACCC CCGAGAGCCT GACGGAGCAG
    GTGGCCATGA CGCTGGCCTC GGCCATCAGC GAGGGCACTG TGCTTACGGC CCGCTCGGGC
    ACCGGTGGCG CTGAGCAGGC CACTGTGACC ATGGTTTCGT CGGAGGACAT TGAGATCCTG
    GAGCATGCTG GCGAGCTAGT TATCGCCTCC CCGGAGGGCC AGCTTGAGGT GCAGACGGTC
    ATCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32740
    Clone ID Related Accession (Same CDS sequence) XM_005224453.3 , XM_005224453.4
    Accession Version XM_005224453.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224453.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGGAGGC
    GGACACATCA AAGAGGTCAT CGTGGCTTCA GAGGCAGAGC CGGGGGACAG CGAGATGGCC
    GAGGGCCCAG GCAGCTCCAG TGAGCGGGGG CCCAGGCTCG CTGCAGAGGG CGAGCAGGCC
    CAGGTCAAAC TGCTGGTGAA CAAGGACGGC CGCTACGTGT GCATGCTCTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACGC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAAC TGTGTGGGGC CTCTTTCCGG ACCAAAGGCT CACTCATCCG GCACCACCGG
    AGGCACACAG ACGAGCGCCC CTATAAGTGT GCCAAGTGTG GGAAGAGCTT CCGGGAGTCG
    GGGGCACTGA CCCGGCACCT GAAGTCTCTC ACCCCGTGTA CAGAGAAAAT CCGCTTCAGC
    ATGAGCAAAG ACGTGGTCGT CGGCAAAGAG GACGTGCCTG CAGGGTCCGG TGCCTCCACT
    GTGGGGCCTG TCACCCCGTC GTCGGCAGGT GAGCCCATGG AGACGTCGCC GGTGATTCAC
    CTGGTGACAG ATGCCAAGGG CACCGTCATC CATGAAGTCC ACGTCCAGAT GCAAGAGCTT
    CCCCTGGGCA TGAAGGCCCT CGCCCCGGAG CCCACCAGCC CCCAGGAGCT TCCCTGCTCC
    AGCGAGGGTG GCCGTGAGAA CCTTCTGCAC CAAGCCATGC AGAACTCCGG CATTGTCCTC
    GAGCGGGCCC CCGGGGAGGA GGGGGCCCTG GGGCCAGCCA CCCCCACCGC GTCCAGTCCC
    CAGTCGCCAG GAGACGCTGC CCCGGAACTG CCGCTGCTGG AGGTGGAGCA GGCGGAGACG
    CAGGTGGCCG GCGAGGCCTC GTCTGTGCCC AGGACCCACC CGTGCCCTCA GTGCAGTGAG
    ACCTTCCCAA CGGCGGCCAC CCTGGAGGCC CACAAAAGAG GCCATGCAGG GCCGAGGCCC
    TTCACGTGCC CGCAGTGTGG CAAGGCCTTC CCCAAGGCCT ACCTGCTCAA GAAGCACCAG
    GAGGTGCACG TGCACGAGCG CCGCTTCCGC TGCGGCGACT GTGGGAAGCT CTACAAGACC
    ATCGCCCACG TCCGCGGCCA CCGGCGCGTC CACTCGGACG AGCGGCCCTA CCCCTGTCCT
    GAGTGCGGCA AGTGCTACAA GACCAAGAAC GCCCAGCAGG TGCACTTCCG GACGCACCTA
    GAGGAGAAGC CGCATGTGTG CCCGTTCTGC AGCCGCGGCT TCCGGGAGAA GGGCTCGCTG
    GTGCGGCACG TGCGGCACCA CACGGGCGAG AAGCCCTTCA AGTGCTACAA GTGTGGCCGC
    GGCTTCGCCG AGCACGGCAC ACTCAACCGG CACCTGCGGA CCAAAGGGGG CTGCCTGTTG
    GAGGTGGAGG AGCTGCTGGT GTCTGAAGAG AGCCCCACTG CGGCCGCCGC CGTCCTGACC
    GAGGACCCAC ACACCGTGTT GGTCGAGTTC TCGTCTGTGG TAGCCGACAC GCAGGAGTAC
    ATCATAGAGG CCACTGCAGA TGATGCAGAG ACCAGCGAAG CCACGGAGAT CATCGAGGGC
    ACCCAGACGG AGGTGGACAG TCACATCATG AAGGTGGTGC AGCAGATAGT GCACCAGGCC
    AGCGCTGGGC ACCAGATCAT CGTGCAGAAC GTGACCATGG ACCAGGAGGC GCGGCTGGGC
    CCAGAGGCGG CTGCAGCCGA CACCATCACC ATCGCCACCC CCGAGAGCCT GACGGAGCAG
    GTGGCCATGA CGCTGGCCTC GGCCATCAGC GAGGGCACTG TGCTTACGGC CCGCTCGGGC
    ACCGGTGGCG CTGAGCAGGC CACTGTGACC ATGGTTTCGT CGGAGGACAT TGAGATCCTG
    GAGCATGCTG GCGAGCTAGT TATCGCCTCC CCGGAGGGCC AGCTTGAGGT GCAGACGGTC
    ATCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224510.1
    CDS362..2710
    Translation

    Target ORF information:

    RefSeq Version XM_005224453.4
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224453.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGCAGG TGGCACCAGG AGCAGCCAGC TCAGAGGAGC CCATCACTGT GGCCCACATC
    GTGGTGGAGG CGGCTGCGCT TGCAGGAGAC ATTAACCACT CGCCTGACAT AGTTGGAGGC
    GGACACATCA AAGAGGTCAT CGTGGCTTCA GAGGCAGAGC CGGGGGACAG CGAGATGGCC
    GAGGGCCCAG GCAGCTCCAG TGAGCGGGGG CCCAGGCTCG CTGCAGAGGG CGAGCAGGCC
    CAGGTCAAAC TGCTGGTGAA CAAGGACGGC CGCTACGTGT GCATGCTCTG CCACAAGACC
    TTCAAGACGG GCAGCATCCT CAAGGCCCAC ATGGTCACGC ACAGCAGCCG CAAGGACCAC
    GAGTGCAAAC TGTGTGGGGC CTCTTTCCGG ACCAAAGGCT CACTCATCCG GCACCACCGG
    AGGCACACAG ACGAGCGCCC CTATAAGTGT GCCAAGTGTG GGAAGAGCTT CCGGGAGTCG
    GGGGCACTGA CCCGGCACCT GAAGTCTCTC ACCCCGTGTA CAGAGAAAAT CCGCTTCAGC
    ATGAGCAAAG ACGTGGTCGT CGGCAAAGAG GACGTGCCTG CAGGGTCCGG TGCCTCCACT
    GTGGGGCCTG TCACCCCGTC GTCGGCAGGT GAGCCCATGG AGACGTCGCC GGTGATTCAC
    CTGGTGACAG ATGCCAAGGG CACCGTCATC CATGAAGTCC ACGTCCAGAT GCAAGAGCTT
    CCCCTGGGCA TGAAGGCCCT CGCCCCGGAG CCCACCAGCC CCCAGGAGCT TCCCTGCTCC
    AGCGAGGGTG GCCGTGAGAA CCTTCTGCAC CAAGCCATGC AGAACTCCGG CATTGTCCTC
    GAGCGGGCCC CCGGGGAGGA GGGGGCCCTG GGGCCAGCCA CCCCCACCGC GTCCAGTCCC
    CAGTCGCCAG GAGACGCTGC CCCGGAACTG CCGCTGCTGG AGGTGGAGCA GGCGGAGACG
    CAGGTGGCCG GCGAGGCCTC GTCTGTGCCC AGGACCCACC CGTGCCCTCA GTGCAGTGAG
    ACCTTCCCAA CGGCGGCCAC CCTGGAGGCC CACAAAAGAG GCCATGCAGG GCCGAGGCCC
    TTCACGTGCC CGCAGTGTGG CAAGGCCTTC CCCAAGGCCT ACCTGCTCAA GAAGCACCAG
    GAGGTGCACG TGCACGAGCG CCGCTTCCGC TGCGGCGACT GTGGGAAGCT CTACAAGACC
    ATCGCCCACG TCCGCGGCCA CCGGCGCGTC CACTCGGACG AGCGGCCCTA CCCCTGTCCT
    GAGTGCGGCA AGTGCTACAA GACCAAGAAC GCCCAGCAGG TGCACTTCCG GACGCACCTA
    GAGGAGAAGC CGCATGTGTG CCCGTTCTGC AGCCGCGGCT TCCGGGAGAA GGGCTCGCTG
    GTGCGGCACG TGCGGCACCA CACGGGCGAG AAGCCCTTCA AGTGCTACAA GTGTGGCCGC
    GGCTTCGCCG AGCACGGCAC ACTCAACCGG CACCTGCGGA CCAAAGGGGG CTGCCTGTTG
    GAGGTGGAGG AGCTGCTGGT GTCTGAAGAG AGCCCCACTG CGGCCGCCGC CGTCCTGACC
    GAGGACCCAC ACACCGTGTT GGTCGAGTTC TCGTCTGTGG TAGCCGACAC GCAGGAGTAC
    ATCATAGAGG CCACTGCAGA TGATGCAGAG ACCAGCGAAG CCACGGAGAT CATCGAGGGC
    ACCCAGACGG AGGTGGACAG TCACATCATG AAGGTGGTGC AGCAGATAGT GCACCAGGCC
    AGCGCTGGGC ACCAGATCAT CGTGCAGAAC GTGACCATGG ACCAGGAGGC GCGGCTGGGC
    CCAGAGGCGG CTGCAGCCGA CACCATCACC ATCGCCACCC CCGAGAGCCT GACGGAGCAG
    GTGGCCATGA CGCTGGCCTC GGCCATCAGC GAGGGCACTG TGCTTACGGC CCGCTCGGGC
    ACCGGTGGCG CTGAGCAGGC CACTGTGACC ATGGTTTCGT CGGAGGACAT TGAGATCCTG
    GAGCATGCTG GCGAGCTAGT TATCGCCTCC CCGGAGGGCC AGCTTGAGGT GCAGACGGTC
    ATCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32741
    Clone ID Related Accession (Same CDS sequence) XM_005224454.4 , XM_005224454.3
    Accession Version XM_005224454.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224454.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGGTG
    GCCGGCGAGG CCTCGTCTGT GCCCAGGACC CACCCGTGCC CTCAGTGCAG TGAGACCTTC
    CCAACGGCGG CCACCCTGGA GGCCCACAAA AGAGGCCATG CAGGGCCGAG GCCCTTCACG
    TGCCCGCAGT GTGGCAAGGC CTTCCCCAAG GCCTACCTGC TCAAGAAGCA CCAGGAGGTG
    CACGTGCACG AGCGCCGCTT CCGCTGCGGC GACTGTGGGA AGCTCTACAA GACCATCGCC
    CACGTCCGCG GCCACCGGCG CGTCCACTCG GACGAGCGGC CCTACCCCTG TCCTGAGTGC
    GGCAAGTGCT ACAAGACCAA GAACGCCCAG CAGGTGCACT TCCGGACGCA CCTAGAGGAG
    AAGCCGCATG TGTGCCCGTT CTGCAGCCGC GGCTTCCGGG AGAAGGGCTC GCTGGTGCGG
    CACGTGCGGC ACCACACGGG CGAGAAGCCC TTCAAGTGCT ACAAGTGTGG CCGCGGCTTC
    GCCGAGCACG GCACACTCAA CCGGCACCTG CGGACCAAAG GGGGCTGCCT GTTGGAGGTG
    GAGGAGCTGC TGGTGTCTGA AGAGAGCCCC ACTGCGGCCG CCGCCGTCCT GACCGAGGAC
    CCACACACCG TGTTGGTCGA GTTCTCGTCT GTGGTAGCCG ACACGCAGGA GTACATCATA
    GAGGCCACTG CAGATGATGC AGAGACCAGC GAAGCCACGG AGATCATCGA GGGCACCCAG
    ACGGAGGTGG ACAGTCACAT CATGAAGGTG GTGCAGCAGA TAGTGCACCA GGCCAGCGCT
    GGGCACCAGA TCATCGTGCA GAACGTGACC ATGGACCAGG AGGCGCGGCT GGGCCCAGAG
    GCGGCTGCAG CCGACACCAT CACCATCGCC ACCCCCGAGA GCCTGACGGA GCAGGTGGCC
    ATGACGCTGG CCTCGGCCAT CAGCGAGGGC ACTGTGCTTA CGGCCCGCTC GGGCACCGGT
    GGCGCTGAGC AGGCCACTGT GACCATGGTT TCGTCGGAGG ACATTGAGAT CCTGGAGCAT
    GCTGGCGAGC TAGTTATCGC CTCCCCGGAG GGCCAGCTTG AGGTGCAGAC GGTCATCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224511.1
    CDS364..2706
    Translation

    Target ORF information:

    RefSeq Version XM_005224454.4
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224454.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGGTG
    GCCGGCGAGG CCTCGTCTGT GCCCAGGACC CACCCGTGCC CTCAGTGCAG TGAGACCTTC
    CCAACGGCGG CCACCCTGGA GGCCCACAAA AGAGGCCATG CAGGGCCGAG GCCCTTCACG
    TGCCCGCAGT GTGGCAAGGC CTTCCCCAAG GCCTACCTGC TCAAGAAGCA CCAGGAGGTG
    CACGTGCACG AGCGCCGCTT CCGCTGCGGC GACTGTGGGA AGCTCTACAA GACCATCGCC
    CACGTCCGCG GCCACCGGCG CGTCCACTCG GACGAGCGGC CCTACCCCTG TCCTGAGTGC
    GGCAAGTGCT ACAAGACCAA GAACGCCCAG CAGGTGCACT TCCGGACGCA CCTAGAGGAG
    AAGCCGCATG TGTGCCCGTT CTGCAGCCGC GGCTTCCGGG AGAAGGGCTC GCTGGTGCGG
    CACGTGCGGC ACCACACGGG CGAGAAGCCC TTCAAGTGCT ACAAGTGTGG CCGCGGCTTC
    GCCGAGCACG GCACACTCAA CCGGCACCTG CGGACCAAAG GGGGCTGCCT GTTGGAGGTG
    GAGGAGCTGC TGGTGTCTGA AGAGAGCCCC ACTGCGGCCG CCGCCGTCCT GACCGAGGAC
    CCACACACCG TGTTGGTCGA GTTCTCGTCT GTGGTAGCCG ACACGCAGGA GTACATCATA
    GAGGCCACTG CAGATGATGC AGAGACCAGC GAAGCCACGG AGATCATCGA GGGCACCCAG
    ACGGAGGTGG ACAGTCACAT CATGAAGGTG GTGCAGCAGA TAGTGCACCA GGCCAGCGCT
    GGGCACCAGA TCATCGTGCA GAACGTGACC ATGGACCAGG AGGCGCGGCT GGGCCCAGAG
    GCGGCTGCAG CCGACACCAT CACCATCGCC ACCCCCGAGA GCCTGACGGA GCAGGTGGCC
    ATGACGCTGG CCTCGGCCAT CAGCGAGGGC ACTGTGCTTA CGGCCCGCTC GGGCACCGGT
    GGCGCTGAGC AGGCCACTGT GACCATGGTT TCGTCGGAGG ACATTGAGAT CCTGGAGCAT
    GCTGGCGAGC TAGTTATCGC CTCCCCGGAG GGCCAGCTTG AGGTGCAGAC GGTCATCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32741
    Clone ID Related Accession (Same CDS sequence) XM_005224454.4 , XM_005224454.3
    Accession Version XM_005224454.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2343bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGGTG
    GCCGGCGAGG CCTCGTCTGT GCCCAGGACC CACCCGTGCC CTCAGTGCAG TGAGACCTTC
    CCAACGGCGG CCACCCTGGA GGCCCACAAA AGAGGCCATG CAGGGCCGAG GCCCTTCACG
    TGCCCGCAGT GTGGCAAGGC CTTCCCCAAG GCCTACCTGC TCAAGAAGCA CCAGGAGGTG
    CACGTGCACG AGCGCCGCTT CCGCTGCGGC GACTGTGGGA AGCTCTACAA GACCATCGCC
    CACGTCCGCG GCCACCGGCG CGTCCACTCG GACGAGCGGC CCTACCCCTG TCCTGAGTGC
    GGCAAGTGCT ACAAGACCAA GAACGCCCAG CAGGTGCACT TCCGGACGCA CCTAGAGGAG
    AAGCCGCATG TGTGCCCGTT CTGCAGCCGC GGCTTCCGGG AGAAGGGCTC GCTGGTGCGG
    CACGTGCGGC ACCACACGGG CGAGAAGCCC TTCAAGTGCT ACAAGTGTGG CCGCGGCTTC
    GCCGAGCACG GCACACTCAA CCGGCACCTG CGGACCAAAG GGGGCTGCCT GTTGGAGGTG
    GAGGAGCTGC TGGTGTCTGA AGAGAGCCCC ACTGCGGCCG CCGCCGTCCT GACCGAGGAC
    CCACACACCG TGTTGGTCGA GTTCTCGTCT GTGGTAGCCG ACACGCAGGA GTACATCATA
    GAGGCCACTG CAGATGATGC AGAGACCAGC GAAGCCACGG AGATCATCGA GGGCACCCAG
    ACGGAGGTGG ACAGTCACAT CATGAAGGTG GTGCAGCAGA TAGTGCACCA GGCCAGCGCT
    GGGCACCAGA TCATCGTGCA GAACGTGACC ATGGACCAGG AGGCGCGGCT GGGCCCAGAG
    GCGGCTGCAG CCGACACCAT CACCATCGCC ACCCCCGAGA GCCTGACGGA GCAGGTGGCC
    ATGACGCTGG CCTCGGCCAT CAGCGAGGGC ACTGTGCTTA CGGCCCGCTC GGGCACCGGT
    GGCGCTGAGC AGGCCACTGT GACCATGGTT TCGTCGGAGG ACATTGAGAT CCTGGAGCAT
    GCTGGCGAGC TAGTTATCGC CTCCCCGGAG GGCCAGCTTG AGGTGCAGAC GGTCATCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224511.1
    CDS310..2652
    Translation

    Target ORF information:

    RefSeq Version XM_005224454.3
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224454.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGGAGGCGGA
    CACATCAAAG AGGTCATCGT GGCTTCAGAG GCAGAGCCGG GGGACAGCGA GATGGCCGAG
    GGCCCAGGCA GCTCCAGTGA GCGGGGGCCC AGGCTCGCTG CAGAGGGCGA GCAGGCCCAG
    GTCAAACTGC TGGTGAACAA GGACGGCCGC TACGTGTGCA TGCTCTGCCA CAAGACCTTC
    AAGACGGGCA GCATCCTCAA GGCCCACATG GTCACGCACA GCAGCCGCAA GGACCACGAG
    TGCAAACTGT GTGGGGCCTC TTTCCGGACC AAAGGCTCAC TCATCCGGCA CCACCGGAGG
    CACACAGACG AGCGCCCCTA TAAGTGTGCC AAGTGTGGGA AGAGCTTCCG GGAGTCGGGG
    GCACTGACCC GGCACCTGAA GTCTCTCACC CCGTGTACAG AGAAAATCCG CTTCAGCATG
    AGCAAAGACG TGGTCGTCGG CAAAGAGGAC GTGCCTGCAG GGTCCGGTGC CTCCACTGTG
    GGGCCTGTCA CCCCGTCGTC GGCAGGTGAG CCCATGGAGA CGTCGCCGGT GATTCACCTG
    GTGACAGATG CCAAGGGCAC CGTCATCCAT GAAGTCCACG TCCAGATGCA AGAGCTTCCC
    CTGGGCATGA AGGCCCTCGC CCCGGAGCCC ACCAGCCCCC AGGAGCTTCC CTGCTCCAGC
    GAGGGTGGCC GTGAGAACCT TCTGCACCAA GCCATGCAGA ACTCCGGCAT TGTCCTCGAG
    CGGGCCCCCG GGGAGGAGGG GGCCCTGGGG CCAGCCACCC CCACCGCGTC CAGTCCCCAG
    TCGCCAGGAG ACGCTGCCCC GGAACTGCCG CTGCTGGAGG TGGAGCAGGC GGAGACGGTG
    GCCGGCGAGG CCTCGTCTGT GCCCAGGACC CACCCGTGCC CTCAGTGCAG TGAGACCTTC
    CCAACGGCGG CCACCCTGGA GGCCCACAAA AGAGGCCATG CAGGGCCGAG GCCCTTCACG
    TGCCCGCAGT GTGGCAAGGC CTTCCCCAAG GCCTACCTGC TCAAGAAGCA CCAGGAGGTG
    CACGTGCACG AGCGCCGCTT CCGCTGCGGC GACTGTGGGA AGCTCTACAA GACCATCGCC
    CACGTCCGCG GCCACCGGCG CGTCCACTCG GACGAGCGGC CCTACCCCTG TCCTGAGTGC
    GGCAAGTGCT ACAAGACCAA GAACGCCCAG CAGGTGCACT TCCGGACGCA CCTAGAGGAG
    AAGCCGCATG TGTGCCCGTT CTGCAGCCGC GGCTTCCGGG AGAAGGGCTC GCTGGTGCGG
    CACGTGCGGC ACCACACGGG CGAGAAGCCC TTCAAGTGCT ACAAGTGTGG CCGCGGCTTC
    GCCGAGCACG GCACACTCAA CCGGCACCTG CGGACCAAAG GGGGCTGCCT GTTGGAGGTG
    GAGGAGCTGC TGGTGTCTGA AGAGAGCCCC ACTGCGGCCG CCGCCGTCCT GACCGAGGAC
    CCACACACCG TGTTGGTCGA GTTCTCGTCT GTGGTAGCCG ACACGCAGGA GTACATCATA
    GAGGCCACTG CAGATGATGC AGAGACCAGC GAAGCCACGG AGATCATCGA GGGCACCCAG
    ACGGAGGTGG ACAGTCACAT CATGAAGGTG GTGCAGCAGA TAGTGCACCA GGCCAGCGCT
    GGGCACCAGA TCATCGTGCA GAACGTGACC ATGGACCAGG AGGCGCGGCT GGGCCCAGAG
    GCGGCTGCAG CCGACACCAT CACCATCGCC ACCCCCGAGA GCCTGACGGA GCAGGTGGCC
    ATGACGCTGG CCTCGGCCAT CAGCGAGGGC ACTGTGCTTA CGGCCCGCTC GGGCACCGGT
    GGCGCTGAGC AGGCCACTGT GACCATGGTT TCGTCGGAGG ACATTGAGAT CCTGGAGCAT
    GCTGGCGAGC TAGTTATCGC CTCCCCGGAG GGCCAGCTTG AGGTGCAGAC GGTCATCGTC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49489
    Clone ID Related Accession (Same CDS sequence) XM_010819096.3 , XM_010819096.2
    Accession Version XM_010819096.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819096.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGAGGCCTGG
    AGTTCCTCTG GGCTACCCCG TGGATTCGGA GCATCCCTTC CCGCCATCCC TTCTGGCCGA
    GCAGGAGGCG GACACATCAA AGAGGTCATC GTGGCTTCAG AGGCAGAGCC GGGGGACAGC
    GAGATGGCCG AGGGCCCAGG CAGCTCCAGT GAGCGGGGGC CCAGGCTCGC TGCAGAGGGC
    GAGCAGGCCC AGGTCAAACT GCTGGTGAAC AAGGACGGCC GCTACGTGTG CATGCTCTGC
    CACAAGACCT TCAAGACGGG CAGCATCCTC AAGGCCCACA TGGTCACGCA CAGCAGCCGC
    AAGGACCACG AGTGCAAACT GTGTGGGGCC TCTTTCCGGA CCAAAGGCTC ACTCATCCGG
    CACCACCGGA GGCACACAGA CGAGCGCCCC TATAAGTGTG CCAAGTGTGG GAAGAGCTTC
    CGGGAGTCGG GGGCACTGAC CCGGCACCTG AAGTCTCTCA CCCCGTGTAC AGAGAAAATC
    CGCTTCAGCA TGAGCAAAGA CGTGGTCGTC GGCAAAGAGG ACGTGCCTGC AGGGTCCGGT
    GCCTCCACTG TGGGGCCTGT CACCCCGTCG TCGGCAGGTG AGCCCATGGA GACGTCGCCG
    GTGATTCACC TGGTGACAGA TGCCAAGGGC ACCGTCATCC ATGAAGTCCA CGTCCAGATG
    CAAGAGCTTC CCCTGGGCAT GAAGGCCCTC GCCCCGGAGC CCACCAGCCC CCAGGAGCTT
    CCCTGCTCCA GCGAGGGTGG CCGTGAGAAC CTTCTGCACC AAGCCATGCA GAACTCCGGC
    ATTGTCCTCG AGCGGGCCCC CGGGGAGGAG GGGGCCCTGG GGCCAGCCAC CCCCACCGCG
    TCCAGTCCCC AGTCGCCAGG AGACGCTGCC CCGGAACTGC CGCTGCTGGA GGTGGAGCAG
    GCGGAGACGC AGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817398.1
    CDS367..2784
    Translation

    Target ORF information:

    RefSeq Version XM_010819096.3
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819096.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGAGGCCTGG
    AGTTCCTCTG GGCTACCCCG TGGATTCGGA GCATCCCTTC CCGCCATCCC TTCTGGCCGA
    GCAGGAGGCG GACACATCAA AGAGGTCATC GTGGCTTCAG AGGCAGAGCC GGGGGACAGC
    GAGATGGCCG AGGGCCCAGG CAGCTCCAGT GAGCGGGGGC CCAGGCTCGC TGCAGAGGGC
    GAGCAGGCCC AGGTCAAACT GCTGGTGAAC AAGGACGGCC GCTACGTGTG CATGCTCTGC
    CACAAGACCT TCAAGACGGG CAGCATCCTC AAGGCCCACA TGGTCACGCA CAGCAGCCGC
    AAGGACCACG AGTGCAAACT GTGTGGGGCC TCTTTCCGGA CCAAAGGCTC ACTCATCCGG
    CACCACCGGA GGCACACAGA CGAGCGCCCC TATAAGTGTG CCAAGTGTGG GAAGAGCTTC
    CGGGAGTCGG GGGCACTGAC CCGGCACCTG AAGTCTCTCA CCCCGTGTAC AGAGAAAATC
    CGCTTCAGCA TGAGCAAAGA CGTGGTCGTC GGCAAAGAGG ACGTGCCTGC AGGGTCCGGT
    GCCTCCACTG TGGGGCCTGT CACCCCGTCG TCGGCAGGTG AGCCCATGGA GACGTCGCCG
    GTGATTCACC TGGTGACAGA TGCCAAGGGC ACCGTCATCC ATGAAGTCCA CGTCCAGATG
    CAAGAGCTTC CCCTGGGCAT GAAGGCCCTC GCCCCGGAGC CCACCAGCCC CCAGGAGCTT
    CCCTGCTCCA GCGAGGGTGG CCGTGAGAAC CTTCTGCACC AAGCCATGCA GAACTCCGGC
    ATTGTCCTCG AGCGGGCCCC CGGGGAGGAG GGGGCCCTGG GGCCAGCCAC CCCCACCGCG
    TCCAGTCCCC AGTCGCCAGG AGACGCTGCC CCGGAACTGC CGCTGCTGGA GGTGGAGCAG
    GCGGAGACGC AGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa49489
    Clone ID Related Accession (Same CDS sequence) XM_010819096.3 , XM_010819096.2
    Accession Version XM_010819096.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transcription factor E4F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104571.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819096.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGAGGCCTGG
    AGTTCCTCTG GGCTACCCCG TGGATTCGGA GCATCCCTTC CCGCCATCCC TTCTGGCCGA
    GCAGGAGGCG GACACATCAA AGAGGTCATC GTGGCTTCAG AGGCAGAGCC GGGGGACAGC
    GAGATGGCCG AGGGCCCAGG CAGCTCCAGT GAGCGGGGGC CCAGGCTCGC TGCAGAGGGC
    GAGCAGGCCC AGGTCAAACT GCTGGTGAAC AAGGACGGCC GCTACGTGTG CATGCTCTGC
    CACAAGACCT TCAAGACGGG CAGCATCCTC AAGGCCCACA TGGTCACGCA CAGCAGCCGC
    AAGGACCACG AGTGCAAACT GTGTGGGGCC TCTTTCCGGA CCAAAGGCTC ACTCATCCGG
    CACCACCGGA GGCACACAGA CGAGCGCCCC TATAAGTGTG CCAAGTGTGG GAAGAGCTTC
    CGGGAGTCGG GGGCACTGAC CCGGCACCTG AAGTCTCTCA CCCCGTGTAC AGAGAAAATC
    CGCTTCAGCA TGAGCAAAGA CGTGGTCGTC GGCAAAGAGG ACGTGCCTGC AGGGTCCGGT
    GCCTCCACTG TGGGGCCTGT CACCCCGTCG TCGGCAGGTG AGCCCATGGA GACGTCGCCG
    GTGATTCACC TGGTGACAGA TGCCAAGGGC ACCGTCATCC ATGAAGTCCA CGTCCAGATG
    CAAGAGCTTC CCCTGGGCAT GAAGGCCCTC GCCCCGGAGC CCACCAGCCC CCAGGAGCTT
    CCCTGCTCCA GCGAGGGTGG CCGTGAGAAC CTTCTGCACC AAGCCATGCA GAACTCCGGC
    ATTGTCCTCG AGCGGGCCCC CGGGGAGGAG GGGGCCCTGG GGCCAGCCAC CCCCACCGCG
    TCCAGTCCCC AGTCGCCAGG AGACGCTGCC CCGGAACTGC CGCTGCTGGA GGTGGAGCAG
    GCGGAGACGC AGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817398.1
    CDS310..2727
    Translation

    Target ORF information:

    RefSeq Version XM_010819096.2
    Organism Bos taurus(cattle)
    Definition Bos taurus E4F transcription factor 1 (E4F1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819096.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGAGGGCG CGATGGCAGT GCGGGTGACG GCCGCGCATA CGGCAGAAGC CCGGGCCGAA 
    GCCGGGCGGG AAGCGGGCGA GGGCGGGGTC GCGGCGGCGG CGGCGGCCTT GGCCCCTGGT
    GGCTTTCTCG GCCTCCCGGC GCCCTTCAGC GAGGAAGATG AGGACGACGT GCACAGATGC
    GGCCGCTGCC AGGCAGAGTT CACTGCCCTG GAGGACTTTG TTCAGCACAA GCTCCAGAAG
    GCCTGCCAGC GGGCGCCTCA GGAGGCCCTG CCCGCCACCC CGGCGGCTGC GCTGCTGGGT
    CGGGAGGTGG CACCAGGAGC AGCCAGCTCA GAGGAGCCCA TCACTGTGGC CCACATCGTG
    GTGGAGGCGG CTGCGCTTGC AGGAGACATT AACCACTCGC CTGACATAGT TGAGGCCTGG
    AGTTCCTCTG GGCTACCCCG TGGATTCGGA GCATCCCTTC CCGCCATCCC TTCTGGCCGA
    GCAGGAGGCG GACACATCAA AGAGGTCATC GTGGCTTCAG AGGCAGAGCC GGGGGACAGC
    GAGATGGCCG AGGGCCCAGG CAGCTCCAGT GAGCGGGGGC CCAGGCTCGC TGCAGAGGGC
    GAGCAGGCCC AGGTCAAACT GCTGGTGAAC AAGGACGGCC GCTACGTGTG CATGCTCTGC
    CACAAGACCT TCAAGACGGG CAGCATCCTC AAGGCCCACA TGGTCACGCA CAGCAGCCGC
    AAGGACCACG AGTGCAAACT GTGTGGGGCC TCTTTCCGGA CCAAAGGCTC ACTCATCCGG
    CACCACCGGA GGCACACAGA CGAGCGCCCC TATAAGTGTG CCAAGTGTGG GAAGAGCTTC
    CGGGAGTCGG GGGCACTGAC CCGGCACCTG AAGTCTCTCA CCCCGTGTAC AGAGAAAATC
    CGCTTCAGCA TGAGCAAAGA CGTGGTCGTC GGCAAAGAGG ACGTGCCTGC AGGGTCCGGT
    GCCTCCACTG TGGGGCCTGT CACCCCGTCG TCGGCAGGTG AGCCCATGGA GACGTCGCCG
    GTGATTCACC TGGTGACAGA TGCCAAGGGC ACCGTCATCC ATGAAGTCCA CGTCCAGATG
    CAAGAGCTTC CCCTGGGCAT GAAGGCCCTC GCCCCGGAGC CCACCAGCCC CCAGGAGCTT
    CCCTGCTCCA GCGAGGGTGG CCGTGAGAAC CTTCTGCACC AAGCCATGCA GAACTCCGGC
    ATTGTCCTCG AGCGGGCCCC CGGGGAGGAG GGGGCCCTGG GGCCAGCCAC CCCCACCGCG
    TCCAGTCCCC AGTCGCCAGG AGACGCTGCC CCGGAACTGC CGCTGCTGGA GGTGGAGCAG
    GCGGAGACGC AGGTGGCCGG CGAGGCCTCG TCTGTGCCCA GGACCCACCC GTGCCCTCAG
    TGCAGTGAGA CCTTCCCAAC GGCGGCCACC CTGGAGGCCC ACAAAAGAGG CCATGCAGGG
    CCGAGGCCCT TCACGTGCCC GCAGTGTGGC AAGGCCTTCC CCAAGGCCTA CCTGCTCAAG
    AAGCACCAGG AGGTGCACGT GCACGAGCGC CGCTTCCGCT GCGGCGACTG TGGGAAGCTC
    TACAAGACCA TCGCCCACGT CCGCGGCCAC CGGCGCGTCC ACTCGGACGA GCGGCCCTAC
    CCCTGTCCTG AGTGCGGCAA GTGCTACAAG ACCAAGAACG CCCAGCAGGT GCACTTCCGG
    ACGCACCTAG AGGAGAAGCC GCATGTGTGC CCGTTCTGCA GCCGCGGCTT CCGGGAGAAG
    GGCTCGCTGG TGCGGCACGT GCGGCACCAC ACGGGCGAGA AGCCCTTCAA GTGCTACAAG
    TGTGGCCGCG GCTTCGCCGA GCACGGCACA CTCAACCGGC ACCTGCGGAC CAAAGGGGGC
    TGCCTGTTGG AGGTGGAGGA GCTGCTGGTG TCTGAAGAGA GCCCCACTGC GGCCGCCGCC
    GTCCTGACCG AGGACCCACA CACCGTGTTG GTCGAGTTCT CGTCTGTGGT AGCCGACACG
    CAGGAGTACA TCATAGAGGC CACTGCAGAT GATGCAGAGA CCAGCGAAGC CACGGAGATC
    ATCGAGGGCA CCCAGACGGA GGTGGACAGT CACATCATGA AGGTGGTGCA GCAGATAGTG
    CACCAGGCCA GCGCTGGGCA CCAGATCATC GTGCAGAACG TGACCATGGA CCAGGAGGCG
    CGGCTGGGCC CAGAGGCGGC TGCAGCCGAC ACCATCACCA TCGCCACCCC CGAGAGCCTG
    ACGGAGCAGG TGGCCATGAC GCTGGCCTCG GCCATCAGCG AGGGCACTGT GCTTACGGCC
    CGCTCGGGCA CCGGTGGCGC TGAGCAGGCC ACTGTGACCA TGGTTTCGTC GGAGGACATT
    GAGATCCTGG AGCATGCTGG CGAGCTAGTT ATCGCCTCCC CGGAGGGCCA GCTTGAGGTG
    CAGACGGTCA TCGTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.