CARS cDNA ORF clone, Bos taurus(cattle)

The following CARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa231971 XM_024987144.1
Latest version!
Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa39791 NM_001103249.1
Latest version!
Bos taurus cysteinyl-tRNA synthetase 1 (CARS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00
OBa89150 XM_015461344.1
Latest version!
Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $426.30
$609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa231971
    Clone ID Related Accession (Same CDS sequence) XM_024987144.1
    Accession Version XM_024987144.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product cysteine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037356.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGGGT CTACCACGGA GCACGCAGGC GGAGGGCGGC GTGCACAGCC CACATGGTCT 
    CCTCCAGCTG GGTCTGAGCC ATGCCAGCTG CGCCTGTACA ACAGCCTGAC ACGGAGGAAG
    GACGTGTTTG TGCCGCAAGA CGGAAGGAGG GTGACATGGT ACTGCTGCGG GCCCACCGTC
    TACGACGCCT CGCACATGGG GCACGCCAGG TCCTACATCT CCTTCGATAT CCTGAGAAGA
    GTGCTGAGGG ATTACTTCAA ATTTGACGTC TTCTACTGCA TGAACATCAC GGACATTGAC
    GATAAGATCA TCAAGAGGGC CCGGCAGAAC TTCCTGTTGG AGCGGTACCG GGAGACGCAG
    CCCCAGGCAG CGCAGCTGCT GGAGGACGTG CGAGCCGCCC TGCAGCCGTT CTCCGCGAAG
    CTCCAGGAGA CTGTGGACCC GGACAAGAAG CAGATGCTGG AACGCCTGCA GCACGCGGTG
    GCGCAGGCCG CGGAGCCGCT GGAGGGCGCG CTGCGGGCCG GGCAGGCCGG GCAGGAGCTG
    GACGGCCGCG TGCAGGCGTT GCTGGAAGAA GCAAAGGATG TGCTTTCTGA CTGGCTGGAT
    TCCATGCACG GCAGTGAGGT GACGGACAAT TCTATTTTCT CTGAACTGCC CAAATTCTGG
    GAGGCGGAGT TCCACAAAGA CATGGAAGCC CTGAACGTCC TCCCTCCGGA CGTCCTGACC
    CGGGTTAGTG AGTATGTGCC AGAGATCGTG GACTTTGTCC AGAAGATCGT GGACAACGGT
    TACGGGTATG CTTCAAACGG GTCTGTGTAC TTCGACACAG TCAAGTTTGC CAACACTGAG
    GGGCACAGCT ACGGAAAGCT GGTGCCCGAG GCCGTCGGGG ACCAGAAAGC CCTGCACGAG
    GGGGAAGGCG ACCTGAGCGT CTCTGCAGAG CGCCTGAGTG AGAAGCGGTC CCCCAATGAC
    TTTGCGCTCT GGAAGGCCTC CAAGCCCGGG GAGCCGTCCT GGCCCTGCCC CTGGGGCAAG
    GGGCGCCCCG GATGGCACAT CGAGTGCTCG GCCATGGCGG GCACCCTCCT GGGAGCCTCG
    ATGGACATCC ACGGAGGGGG CTTCGACCTC CGCTTCCCTC ACCACGACAA CGAGCTGGCG
    CAGTCAGAGG CCTACTTTGA GAACGACTGC TGGGTCAGGT ACTTCCTGCA CACAGGCCAC
    CTGACCATCG CGGGCTGCAA GATGTCCAAG TCGCTCAAGA ACTTCATCAC CATTAAAGAC
    GCCCTGAAGA AGCACTCAGC CCGCCAGCTG CGCCTGGCCT TCCTCATGCA CTCGTGGAAG
    GACACGCTGG ACTACTCGGC CAACACCATG GAGTCGGCGC TGCAGTACGA GAGGCTCCTC
    AATGAGTTTT TCTTAAACGT GAAAGACCTC CTCCGAGCCC CCGTGGAGGT GGCCGCTCAG
    TTTGAGAAGT GGGAGGACGA GGAAACGGAG CTGAACAAGA GCTTTTACAA GAAGAAGGCC
    GCGGTTCACC AGGCGCTCTG TGACAACATC GACACGCGCA CAGTCCTGGA GGAGATGCGG
    GCTCTGGTCG GCCAGTGCAA CCTCTACATG GCGGCCCGCA AGGCCGCCCG GAGGAGGCCC
    CACCGCGTCC TGCTGGAGAG CATCGCCCAC TACCTCACAC ACATGCTGAA GATCTTCGGG
    GCCATAGAAG AGGAAAGCTC CTTGGGTTTC CCCATCGGAG GAGCTGGAAC CAGCCTCAAT
    CTGGAGTCCA CGGTCATGCC CTACCTGCAG GTGCTGTCGG AGTTCCGGGA AGGGGTGCGG
    CGGATCGCCC GTGAGCACAG AGTGCCTGAG GTCCTCCAGC TGAGCGACGC CCTGCGGGAC
    AACGTCCTGC CCGAGCTGGG GGTGCGGCTG GAAGACCACG AAGGTCTGCC CACCGTGGTC
    AAGCTGGTGG ACAGAGACGC CTTACTGAGA GAGAGAGAAG ACAAGAAAAG GGCGGAAGAG
    GAGAAAAGGA AGAAGAAAGA GGAGGCTACC AGGAAGAGAC AGGAGCAGGA GGCAGCAAAG
    CTGGCCAAGA TGAAGATCCC ACCCAGTGAG ATGTTCTTAT CGGAAAGCGA CAAATACTCC
    AAGTTCGACG AAAATGGTCT GCCCACGCAC GACACCGAAG GCAAAGAGCT GAGCAAGGGG
    CAGGCCAAGA AGCTGCGGAA GCTGTTCGAG GCGCAGGAGA AGCTGCACTT AGAGTACCTG
    CAACTGGTTC AGAACGGGGG CCCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842912.1
    CDS55..2304
    Translation

    Target ORF information:

    RefSeq Version XM_024987144.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024987144.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCAGGGT CTACCACGGA GCACGCAGGC GGAGGGCGGC GTGCACAGCC CACATGGTCT 
    CCTCCAGCTG GGTCTGAGCC ATGCCAGCTG CGCCTGTACA ACAGCCTGAC ACGGAGGAAG
    GACGTGTTTG TGCCGCAAGA CGGAAGGAGG GTGACATGGT ACTGCTGCGG GCCCACCGTC
    TACGACGCCT CGCACATGGG GCACGCCAGG TCCTACATCT CCTTCGATAT CCTGAGAAGA
    GTGCTGAGGG ATTACTTCAA ATTTGACGTC TTCTACTGCA TGAACATCAC GGACATTGAC
    GATAAGATCA TCAAGAGGGC CCGGCAGAAC TTCCTGTTGG AGCGGTACCG GGAGACGCAG
    CCCCAGGCAG CGCAGCTGCT GGAGGACGTG CGAGCCGCCC TGCAGCCGTT CTCCGCGAAG
    CTCCAGGAGA CTGTGGACCC GGACAAGAAG CAGATGCTGG AACGCCTGCA GCACGCGGTG
    GCGCAGGCCG CGGAGCCGCT GGAGGGCGCG CTGCGGGCCG GGCAGGCCGG GCAGGAGCTG
    GACGGCCGCG TGCAGGCGTT GCTGGAAGAA GCAAAGGATG TGCTTTCTGA CTGGCTGGAT
    TCCATGCACG GCAGTGAGGT GACGGACAAT TCTATTTTCT CTGAACTGCC CAAATTCTGG
    GAGGCGGAGT TCCACAAAGA CATGGAAGCC CTGAACGTCC TCCCTCCGGA CGTCCTGACC
    CGGGTTAGTG AGTATGTGCC AGAGATCGTG GACTTTGTCC AGAAGATCGT GGACAACGGT
    TACGGGTATG CTTCAAACGG GTCTGTGTAC TTCGACACAG TCAAGTTTGC CAACACTGAG
    GGGCACAGCT ACGGAAAGCT GGTGCCCGAG GCCGTCGGGG ACCAGAAAGC CCTGCACGAG
    GGGGAAGGCG ACCTGAGCGT CTCTGCAGAG CGCCTGAGTG AGAAGCGGTC CCCCAATGAC
    TTTGCGCTCT GGAAGGCCTC CAAGCCCGGG GAGCCGTCCT GGCCCTGCCC CTGGGGCAAG
    GGGCGCCCCG GATGGCACAT CGAGTGCTCG GCCATGGCGG GCACCCTCCT GGGAGCCTCG
    ATGGACATCC ACGGAGGGGG CTTCGACCTC CGCTTCCCTC ACCACGACAA CGAGCTGGCG
    CAGTCAGAGG CCTACTTTGA GAACGACTGC TGGGTCAGGT ACTTCCTGCA CACAGGCCAC
    CTGACCATCG CGGGCTGCAA GATGTCCAAG TCGCTCAAGA ACTTCATCAC CATTAAAGAC
    GCCCTGAAGA AGCACTCAGC CCGCCAGCTG CGCCTGGCCT TCCTCATGCA CTCGTGGAAG
    GACACGCTGG ACTACTCGGC CAACACCATG GAGTCGGCGC TGCAGTACGA GAGGCTCCTC
    AATGAGTTTT TCTTAAACGT GAAAGACCTC CTCCGAGCCC CCGTGGAGGT GGCCGCTCAG
    TTTGAGAAGT GGGAGGACGA GGAAACGGAG CTGAACAAGA GCTTTTACAA GAAGAAGGCC
    GCGGTTCACC AGGCGCTCTG TGACAACATC GACACGCGCA CAGTCCTGGA GGAGATGCGG
    GCTCTGGTCG GCCAGTGCAA CCTCTACATG GCGGCCCGCA AGGCCGCCCG GAGGAGGCCC
    CACCGCGTCC TGCTGGAGAG CATCGCCCAC TACCTCACAC ACATGCTGAA GATCTTCGGG
    GCCATAGAAG AGGAAAGCTC CTTGGGTTTC CCCATCGGAG GAGCTGGAAC CAGCCTCAAT
    CTGGAGTCCA CGGTCATGCC CTACCTGCAG GTGCTGTCGG AGTTCCGGGA AGGGGTGCGG
    CGGATCGCCC GTGAGCACAG AGTGCCTGAG GTCCTCCAGC TGAGCGACGC CCTGCGGGAC
    AACGTCCTGC CCGAGCTGGG GGTGCGGCTG GAAGACCACG AAGGTCTGCC CACCGTGGTC
    AAGCTGGTGG ACAGAGACGC CTTACTGAGA GAGAGAGAAG ACAAGAAAAG GGCGGAAGAG
    GAGAAAAGGA AGAAGAAAGA GGAGGCTACC AGGAAGAGAC AGGAGCAGGA GGCAGCAAAG
    CTGGCCAAGA TGAAGATCCC ACCCAGTGAG ATGTTCTTAT CGGAAAGCGA CAAATACTCC
    AAGTTCGACG AAAATGGTCT GCCCACGCAC GACACCGAAG GCAAAGAGCT GAGCAAGGGG
    CAGGCCAAGA AGCTGCGGAA GCTGTTCGAG GCGCAGGAGA AGCTGCACTT AGAGTACCTG
    CAACTGGTTC AGAACGGGGG CCCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39791
    Clone ID Related Accession (Same CDS sequence) NM_001103249.1
    Accession Version NM_001103249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Bos taurus(cattle)
    Product cysteine--tRNA ligase, cytoplasmic
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114705.1. On Sep 13, 2007 this sequence version replaced XR_028643.1. ##Evidence-Data-START## Transcript exon combination :: BC114705.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
    GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC
    TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC
    CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC
    CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAACAGGCG GAGGGCGGCG TGCACAGCCC
    ACATGGTCTC CTCCAGCTGG GTCTGAGCCA TGCCAGCTGC GCCTGTACAA CAGCCTGACA
    CGGAGGAAGG ACGTGTTTGT GCCGCAAGAC GGAAGGAGGG TGACATGGTA CTGCTGCGGG
    CCCACCGTCT ACGACGCCTC GCACATGGGG CACGCCAGGT CCTACATCTC CTTCGATATC
    TTGAGAAGAG TGCTGAGGGA TTACTTCAAA TTTGACGTCT TCTACTGCAT GAACATCACG
    GACATTGACG ATAAGATCAT CAAGAGGGCC CGGCAGAACT TCCTGTTGGA GCGGTACCGG
    GAGACGCAGC CCCAGGCAGC GCAGCTGCTG GAGGACGTGC GAGCCGCCCT GCAGCCGTTC
    TCCGCGAAGC TCCAGGAGAC TGTGGACCCG GACAAGAAGC AGATGCTGGA ACGCCTGCAG
    CACGCGGTGG CGCAGGCCGC GGAGCCGCTG GAGGGCGCGC TGCGGGCCGG GCAGGCCGGG
    CAGGAGCTGG ACGGCCGCGT GCAGGCGTTG CTGGAAGAAG CAAAGGATGT GCTTTCTGAC
    TGGCTGGATT CCATGCACGG CAGTGAGGTG ACGGACAATT CTATTTTCTC TGAACTGCCC
    AAATTCTGGG AGGCGGAGTT CCACAAAGAC ATGGAAGCCC TGAACGTCCT CCCTCCGGAC
    GTCCTGACCC GGGTTAGTGA GTATGTGCCA GAGATCGTGG ACTTTGTCCA GAAGATCGTG
    GACAACGGTT ACGGGTATGC TTCAAACGGG TCTGTGTACT TCGACACAGT CAAGTTTGCC
    AACACTGAGG GGCACAGCTA CGGAAAGCTG GTGCCCGAGG CCGTCGGGGA CCAGAAAGCC
    CTGCACGAGG GGGAAGGCGA CCTGAGCGTC TCTGCAGAGC GCCTGAGTGA GAAGCGGTCC
    CCCAATGACT TTGCGCTCTG GAAGGCCTCC AAGCCCGGGG AGCCGTCCTG GCCCTGCCCC
    TGGGGCAAGG GGCGCCCCGG ATGGCACATC GAGTGCTCGG CCATGGCGGG CACCCTCCTG
    GGAGCCTCGA TGGACATCCA CGGAGGGGGC TTCGACCTCC GCTTCCCTCA CCACGACAAC
    GAGCTGGCGC AGTCAGAGGC CTACTTTGAG AACGACTGCT GGGTCAGGTA CTTCCTGCAC
    ACAGGCCACC TGACCATCGC GGGCTGCAAG ATGTCCAAGT CGCTCAAGAA CTTCATCACC
    ATTAAAGACG CCCTGAAGAA GCACTCAGCC CGCCAGCTGC GCCTGGCCTT CCTCATGCAC
    TCGTGGAAGG ACACGCTGGA CTACTCGGCC AACACCATGG AGTCGGCGCT GCAGTACGAG
    AGGCTCCTCA ATGAGTTTTT CTTAAACGTG AAAGACCTCC TCCGAGCCCC CGTGGAGGTG
    GCCGCTCAGT TTGAGAAGTG GGAGGACGAG GAAACGGAGC TGAACAAGAG CTTTTACAAG
    AAGAAGGCCG CGGTTCACCA GGCGCTCTGT GACAACATCG ACACGCGCAC AGTCCTGGAG
    GAGATGCGGG CTCTGGTCGG CCAGTGCAAC CTCTACATGG CGGCCCGCAA GGCCGCCCGG
    AGGAGGCCCC ACCGCGTCCT GCTGGAGAGC ATCGCCCACT ACCTCACACA CATGCTGAAG
    ATCTTCGGGG CCATAGAAGA GGAAAGCTCC TTGGGTTTCC CCATCGGAGG AGCTGGAACC
    AGCCTCAATC TGGAGTCCAC GGTCATGCCC TACCTGCAGG TGCTGTCGGA GTTCCGGGAA
    GGGGTGCGGC GGATCGCCCG TGAGCACAGA GTGCCTGAGG TCCTCCAGCT GAGCGACGCC
    CTGCGGGACA ACGTCCTGCC CGAGCTGGGG GTGCGGCTGG AAGACCACGA AGGTCTGCCC
    ACCGTGGTCA AGCTGGTGGA CAGAGACGCC TTACTGAGAG AGAGAGAAGA CAAGAAAAGG
    GCGGAAGAGG AGAAAAGGAA GAAGAAAGAG GAGGCTACCA GGAAGAGACA GGAGCAGGAG
    GCAGCAAAGC TGGCCAAGAT GAAGATCCCA CCCAGTGAGA TGTTCTTATC GGAAAGCGAC
    AAATACTCCA AGTTCGACGA AAATGGTCTG CCCACGCACG ACACCGAAGG CAAAGAGCTG
    AGCAAGGGGC AGGCCAAGAA GCTGCGGAAG CTGTTCGAGG CGCAGGAGAA GCTGCACTTA
    GAGTACCTGC AACTGGTTCA GAACGGGGGC CCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096719.1
    CDS5..2503
    Translation

    Target ORF information:

    RefSeq Version NM_001103249.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cysteinyl-tRNA synthetase 1 (CARS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
    GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC
    TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC
    CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC
    CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAACAGGCG GAGGGCGGCG TGCACAGCCC
    ACATGGTCTC CTCCAGCTGG GTCTGAGCCA TGCCAGCTGC GCCTGTACAA CAGCCTGACA
    CGGAGGAAGG ACGTGTTTGT GCCGCAAGAC GGAAGGAGGG TGACATGGTA CTGCTGCGGG
    CCCACCGTCT ACGACGCCTC GCACATGGGG CACGCCAGGT CCTACATCTC CTTCGATATC
    TTGAGAAGAG TGCTGAGGGA TTACTTCAAA TTTGACGTCT TCTACTGCAT GAACATCACG
    GACATTGACG ATAAGATCAT CAAGAGGGCC CGGCAGAACT TCCTGTTGGA GCGGTACCGG
    GAGACGCAGC CCCAGGCAGC GCAGCTGCTG GAGGACGTGC GAGCCGCCCT GCAGCCGTTC
    TCCGCGAAGC TCCAGGAGAC TGTGGACCCG GACAAGAAGC AGATGCTGGA ACGCCTGCAG
    CACGCGGTGG CGCAGGCCGC GGAGCCGCTG GAGGGCGCGC TGCGGGCCGG GCAGGCCGGG
    CAGGAGCTGG ACGGCCGCGT GCAGGCGTTG CTGGAAGAAG CAAAGGATGT GCTTTCTGAC
    TGGCTGGATT CCATGCACGG CAGTGAGGTG ACGGACAATT CTATTTTCTC TGAACTGCCC
    AAATTCTGGG AGGCGGAGTT CCACAAAGAC ATGGAAGCCC TGAACGTCCT CCCTCCGGAC
    GTCCTGACCC GGGTTAGTGA GTATGTGCCA GAGATCGTGG ACTTTGTCCA GAAGATCGTG
    GACAACGGTT ACGGGTATGC TTCAAACGGG TCTGTGTACT TCGACACAGT CAAGTTTGCC
    AACACTGAGG GGCACAGCTA CGGAAAGCTG GTGCCCGAGG CCGTCGGGGA CCAGAAAGCC
    CTGCACGAGG GGGAAGGCGA CCTGAGCGTC TCTGCAGAGC GCCTGAGTGA GAAGCGGTCC
    CCCAATGACT TTGCGCTCTG GAAGGCCTCC AAGCCCGGGG AGCCGTCCTG GCCCTGCCCC
    TGGGGCAAGG GGCGCCCCGG ATGGCACATC GAGTGCTCGG CCATGGCGGG CACCCTCCTG
    GGAGCCTCGA TGGACATCCA CGGAGGGGGC TTCGACCTCC GCTTCCCTCA CCACGACAAC
    GAGCTGGCGC AGTCAGAGGC CTACTTTGAG AACGACTGCT GGGTCAGGTA CTTCCTGCAC
    ACAGGCCACC TGACCATCGC GGGCTGCAAG ATGTCCAAGT CGCTCAAGAA CTTCATCACC
    ATTAAAGACG CCCTGAAGAA GCACTCAGCC CGCCAGCTGC GCCTGGCCTT CCTCATGCAC
    TCGTGGAAGG ACACGCTGGA CTACTCGGCC AACACCATGG AGTCGGCGCT GCAGTACGAG
    AGGCTCCTCA ATGAGTTTTT CTTAAACGTG AAAGACCTCC TCCGAGCCCC CGTGGAGGTG
    GCCGCTCAGT TTGAGAAGTG GGAGGACGAG GAAACGGAGC TGAACAAGAG CTTTTACAAG
    AAGAAGGCCG CGGTTCACCA GGCGCTCTGT GACAACATCG ACACGCGCAC AGTCCTGGAG
    GAGATGCGGG CTCTGGTCGG CCAGTGCAAC CTCTACATGG CGGCCCGCAA GGCCGCCCGG
    AGGAGGCCCC ACCGCGTCCT GCTGGAGAGC ATCGCCCACT ACCTCACACA CATGCTGAAG
    ATCTTCGGGG CCATAGAAGA GGAAAGCTCC TTGGGTTTCC CCATCGGAGG AGCTGGAACC
    AGCCTCAATC TGGAGTCCAC GGTCATGCCC TACCTGCAGG TGCTGTCGGA GTTCCGGGAA
    GGGGTGCGGC GGATCGCCCG TGAGCACAGA GTGCCTGAGG TCCTCCAGCT GAGCGACGCC
    CTGCGGGACA ACGTCCTGCC CGAGCTGGGG GTGCGGCTGG AAGACCACGA AGGTCTGCCC
    ACCGTGGTCA AGCTGGTGGA CAGAGACGCC TTACTGAGAG AGAGAGAAGA CAAGAAAAGG
    GCGGAAGAGG AGAAAAGGAA GAAGAAAGAG GAGGCTACCA GGAAGAGACA GGAGCAGGAG
    GCAGCAAAGC TGGCCAAGAT GAAGATCCCA CCCAGTGAGA TGTTCTTATC GGAAAGCGAC
    AAATACTCCA AGTTCGACGA AAATGGTCTG CCCACGCACG ACACCGAAGG CAAAGAGCTG
    AGCAAGGGGC AGGCCAAGAA GCTGCGGAAG CTGTTCGAGG CGCAGGAGAA GCTGCACTTA
    GAGTACCTGC AACTGGTTCA GAACGGGGGC CCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89150
    Clone ID Related Accession (Same CDS sequence) XM_015461344.1
    Accession Version XM_015461344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product cysteine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104648.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
    GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC
    TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC
    CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC
    CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAAGCGGAG GGCGGCGTGC ACAGCCCACA
    TGGTCTCCTC CAGCTGGGTC TGAGCCATGC CAGCTGCGCC TGTACAACAG CCTGACACGG
    AGGAAGGACG TGTTTGTGCC GCAAGACGGA AGGAGGGTGA CATGGTACTG CTGCGGGCCC
    ACCGTCTACG ACGCCTCGCA CATGGGGCAC GCCAGGTCCT ACATCTCCTT CGATATCTTG
    AGAAGAGTGC TGAGGGATTA CTTCAAATTT GACGTCTTCT ACTGCATGAA CATCACGGAC
    ATTGACGATA AGATCATCAA GAGGGCCCGG CAGAACTTCC TGTTGGAGCG GTACCGGGAG
    ACGCAGCCCC AGGCAGCGCA GCTGCTGGAG GACGTGCGAG CCGCCCTGCA GCCGTTCTCC
    GCGAAGCTCC AGGAGACTGT GGACCCGGAC AAGAAGCAGA TGCTGGAACG CCTGCAGCAC
    GCGGTGGCGC AGGCCGCGGA GCCGCTGGAG GGCGCGCTGC GGGCCGGGCA GGCCGGGCAG
    GAGCTGGACG GCCGCGTGCA GGCGTTGCTG GAAGAAGCAA AGGATGTGCT TTCTGACTGG
    CTGGATTCCA TGCACGGCAG TGAGGTGACG GACAATTCTA TTTTCTCTGA ACTGCCCAAA
    TTCTGGGAGG CGGAGTTCCA CAAAGACATG GAAGCCCTGA ACGTCCTCCC TCCGGACGTC
    CTGACCCGGG TTAGTGAGTA TGTGCCAGAG ATCGTGGACT TTGTCCAGAA GATCGTGGAC
    AACGGTTACG GGTATGCTTC AAACGGGTCT GTGTACTTCG ACACAGTCAA GTTTGCCAAC
    ACTGAGGGGC ACAGCTACGG AAAGCTGGTG CCCGAGGCCG TCGGGGACCA GAAAGCCCTG
    CACGAGGGGG AAGGCGACCT GAGCGTCTCT GCAGAGCGCC TGAGTGAGAA GCGGTCCCCC
    AATGACTTTG CGCTCTGGAA GGCCTCCAAG CCCGGGGAGC CGTCCTGGCC CTGCCCCTGG
    GGCAAGGGGC GCCCCGGATG GCACATCGAG TGCTCGGCCA TGGCGGGCAC CCTCCTGGGA
    GCCTCGATGG ACATCCACGG AGGGGGCTTC GACCTCCGCT TCCCTCACCA CGACAACGAG
    CTGGCGCAGT CAGAGGCCTA CTTTGAGAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATCGCGGG CTGCAAGATG TCCAAGTCGC TCAAGAACTT CATCACCATT
    AAAGACGCCC TGAAGAAGCA CTCAGCCCGC CAGCTGCGCC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CGCTGGACTA CTCGGCCAAC ACCATGGAGT CGGCGCTGCA GTACGAGAGG
    CTCCTCAATG AGTTTTTCTT AAACGTGAAA GACCTCCTCC GAGCCCCCGT GGAGGTGGCC
    GCTCAGTTTG AGAAGTGGGA GGACGAGGAA ACGGAGCTGA ACAAGAGCTT TTACAAGAAG
    AAGGCCGCGG TTCACCAGGC GCTCTGTGAC AACATCGACA CGCGCACAGT CCTGGAGGAG
    ATGCGGGCTC TGGTCGGCCA GTGCAACCTC TACATGGCGG CCCGCAAGGC CGCCCGGAGG
    AGGCCCCACC GCGTCCTGCT GGAGAGCATC GCCCACTACC TCACACACAT GCTGAAGATC
    TTCGGGGCCA TAGAAGAGGA AAGCTCCTTG GGTTTCCCCA TCGGAGGAGC TGGAACCAGC
    CTCAATCTGG AGTCCACGGT CATGCCCTAC CTGCAGGTGC TGTCGGAGTT CCGGGAAGGG
    GTGCGGCGGA TCGCCCGTGA GCACAGAGTG CCTGAGGTCC TCCAGCTGAG CGACGCCCTG
    CGGGACAACG TCCTGCCCGA GCTGGGGGTG CGGCTGGAAG ACCACGAAGG TCTGCCCACC
    GTGGTCAAGC TGGTGGACAG AGACGCCTTA CTGAGAGAGA GAGAAGACAA GAAAAGGGCG
    GAAGAGGAGA AAAGGAAGAA GAAAGAGGAG GCTACCAGGA AGAGACAGGA GCAGGAGGCA
    GCAAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTATCGGA AAGCGACAAA
    TACTCCAAGT TCGACGAAAA TGGTCTGCCC ACGCACGACA CCGAAGGCAA AGAGCTGAGC
    AAGGGGCAGG CCAAGAAGCT GCGGAAGCTG TTCGAGGCGC AGGAGAAGCT GCACTTAGAG
    TACCTGCAAC TGGTTCAGAA CGGGGGCCCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015316830.1
    CDS83..2578
    Translation

    Target ORF information:

    RefSeq Version XM_015461344.1
    Organism Bos taurus(cattle)
    Definition Bos taurus cysteinyl-tRNA synthetase (CARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015461344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGGCAGGGT CTACCACGGA GCACGCTCCC GACTACACGG CCATCCTGGG CATTAGTGAC 
    GAGGCGGCCC GGTTGCGGGC GCTGGACGCG TACCTCAGCA CGCGTAGCTA CTTGCAGGGC
    TTTGCCCTGT CTCAGGTGGA CGTGGATGCC TTCAGGCAGC TCTCGGGCCC TCCGGCCGAC
    CCGCAGCTCT TCCACGTGGC CCGCTGGTTC CGGCATGTGA CCGCGATCCT GGGCAGCCCC
    CCCGCCGCAC GCCCGCCCTG CGGGCTCCAA GCAAGCGGAG GGCGGCGTGC ACAGCCCACA
    TGGTCTCCTC CAGCTGGGTC TGAGCCATGC CAGCTGCGCC TGTACAACAG CCTGACACGG
    AGGAAGGACG TGTTTGTGCC GCAAGACGGA AGGAGGGTGA CATGGTACTG CTGCGGGCCC
    ACCGTCTACG ACGCCTCGCA CATGGGGCAC GCCAGGTCCT ACATCTCCTT CGATATCTTG
    AGAAGAGTGC TGAGGGATTA CTTCAAATTT GACGTCTTCT ACTGCATGAA CATCACGGAC
    ATTGACGATA AGATCATCAA GAGGGCCCGG CAGAACTTCC TGTTGGAGCG GTACCGGGAG
    ACGCAGCCCC AGGCAGCGCA GCTGCTGGAG GACGTGCGAG CCGCCCTGCA GCCGTTCTCC
    GCGAAGCTCC AGGAGACTGT GGACCCGGAC AAGAAGCAGA TGCTGGAACG CCTGCAGCAC
    GCGGTGGCGC AGGCCGCGGA GCCGCTGGAG GGCGCGCTGC GGGCCGGGCA GGCCGGGCAG
    GAGCTGGACG GCCGCGTGCA GGCGTTGCTG GAAGAAGCAA AGGATGTGCT TTCTGACTGG
    CTGGATTCCA TGCACGGCAG TGAGGTGACG GACAATTCTA TTTTCTCTGA ACTGCCCAAA
    TTCTGGGAGG CGGAGTTCCA CAAAGACATG GAAGCCCTGA ACGTCCTCCC TCCGGACGTC
    CTGACCCGGG TTAGTGAGTA TGTGCCAGAG ATCGTGGACT TTGTCCAGAA GATCGTGGAC
    AACGGTTACG GGTATGCTTC AAACGGGTCT GTGTACTTCG ACACAGTCAA GTTTGCCAAC
    ACTGAGGGGC ACAGCTACGG AAAGCTGGTG CCCGAGGCCG TCGGGGACCA GAAAGCCCTG
    CACGAGGGGG AAGGCGACCT GAGCGTCTCT GCAGAGCGCC TGAGTGAGAA GCGGTCCCCC
    AATGACTTTG CGCTCTGGAA GGCCTCCAAG CCCGGGGAGC CGTCCTGGCC CTGCCCCTGG
    GGCAAGGGGC GCCCCGGATG GCACATCGAG TGCTCGGCCA TGGCGGGCAC CCTCCTGGGA
    GCCTCGATGG ACATCCACGG AGGGGGCTTC GACCTCCGCT TCCCTCACCA CGACAACGAG
    CTGGCGCAGT CAGAGGCCTA CTTTGAGAAC GACTGCTGGG TCAGGTACTT CCTGCACACA
    GGCCACCTGA CCATCGCGGG CTGCAAGATG TCCAAGTCGC TCAAGAACTT CATCACCATT
    AAAGACGCCC TGAAGAAGCA CTCAGCCCGC CAGCTGCGCC TGGCCTTCCT CATGCACTCG
    TGGAAGGACA CGCTGGACTA CTCGGCCAAC ACCATGGAGT CGGCGCTGCA GTACGAGAGG
    CTCCTCAATG AGTTTTTCTT AAACGTGAAA GACCTCCTCC GAGCCCCCGT GGAGGTGGCC
    GCTCAGTTTG AGAAGTGGGA GGACGAGGAA ACGGAGCTGA ACAAGAGCTT TTACAAGAAG
    AAGGCCGCGG TTCACCAGGC GCTCTGTGAC AACATCGACA CGCGCACAGT CCTGGAGGAG
    ATGCGGGCTC TGGTCGGCCA GTGCAACCTC TACATGGCGG CCCGCAAGGC CGCCCGGAGG
    AGGCCCCACC GCGTCCTGCT GGAGAGCATC GCCCACTACC TCACACACAT GCTGAAGATC
    TTCGGGGCCA TAGAAGAGGA AAGCTCCTTG GGTTTCCCCA TCGGAGGAGC TGGAACCAGC
    CTCAATCTGG AGTCCACGGT CATGCCCTAC CTGCAGGTGC TGTCGGAGTT CCGGGAAGGG
    GTGCGGCGGA TCGCCCGTGA GCACAGAGTG CCTGAGGTCC TCCAGCTGAG CGACGCCCTG
    CGGGACAACG TCCTGCCCGA GCTGGGGGTG CGGCTGGAAG ACCACGAAGG TCTGCCCACC
    GTGGTCAAGC TGGTGGACAG AGACGCCTTA CTGAGAGAGA GAGAAGACAA GAAAAGGGCG
    GAAGAGGAGA AAAGGAAGAA GAAAGAGGAG GCTACCAGGA AGAGACAGGA GCAGGAGGCA
    GCAAAGCTGG CCAAGATGAA GATCCCACCC AGTGAGATGT TCTTATCGGA AAGCGACAAA
    TACTCCAAGT TCGACGAAAA TGGTCTGCCC ACGCACGACA CCGAAGGCAA AGAGCTGAGC
    AAGGGGCAGG CCAAGAAGCT GCGGAAGCTG TTCGAGGCGC AGGAGAAGCT GCACTTAGAG
    TACCTGCAAC TGGTTCAGAA CGGGGGCCCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.