MROH1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MROH1
Entrez Gene ID	515055
Full Name	maestro heat like repeat family member 1
Synonyms	HEATR7A
General protein information	Preferred Names maestro heat like repeat family member 1 Names maestro heat-like repeat-containing protein family member 1 HEAT repeat containing 7A HEAT repeat-containing protein 7A
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010811842.2	XP_010810144.1	maestro heat-like repeat-containing protein family member 1 isoform X3
XM_010811846.2	XP_010810148.1	maestro heat-like repeat-containing protein family member 1 isoform X7
XM_005215189.3	XP_005215246.2	maestro heat-like repeat-containing protein family member 1 isoform X8
XM_005215190.3	XP_005215247.2	maestro heat-like repeat-containing protein family member 1 isoform X9
XM_010811847.2	XP_010810149.1	maestro heat-like repeat-containing protein family member 1 isoform X10
XM_025001535.1	XP_024857303.1	maestro heat-like repeat-containing protein family member 1 isoform X7
XM_025001536.1	XP_024857304.1	maestro heat-like repeat-containing protein family member 1 isoform X8
XM_025001539.1	XP_024857307.1	maestro heat-like repeat-containing protein family member 1 isoform X10
XM_025001544.1	XP_024857312.1	maestro heat-like repeat-containing protein family member 1 isoform X15
XM_010811844.2	XP_010810146.1	maestro heat-like repeat-containing protein family member 1 isoform X5
XM_010811840.2	XP_010810142.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_025001530.1	XP_024857298.1	maestro heat-like repeat-containing protein family member 1 isoform X2
XM_025001531.1	XP_024857299.1	maestro heat-like repeat-containing protein family member 1 isoform X3
NM_001102018.1	NP_001095488.1	maestro heat-like repeat-containing protein family member 1
XM_010811837.2	XP_010810139.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_010811841.2	XP_010810143.1	maestro heat-like repeat-containing protein family member 1 isoform X2
XM_010811845.2	XP_010810147.1	maestro heat-like repeat-containing protein family member 1 isoform X6
XM_025001532.1	XP_024857300.1	maestro heat-like repeat-containing protein family member 1 isoform X4
XM_025001533.1	XP_024857301.1	maestro heat-like repeat-containing protein family member 1 isoform X5
XM_025001534.1	XP_024857302.1	maestro heat-like repeat-containing protein family member 1 isoform X6
XM_025001542.1	XP_024857310.1	maestro heat-like repeat-containing protein family member 1 isoform X13
XM_005215189.4	XP_005215246.2	maestro heat-like repeat-containing protein family member 1 isoform X16
XM_025001545.1	XP_024857313.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_025001547.1	XP_024857315.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_010811843.2	XP_010810145.1	maestro heat-like repeat-containing protein family member 1 isoform X4
XM_010811839.2	XP_010810141.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_025001529.1	XP_024857297.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_025001540.1	XP_024857308.1	maestro heat-like repeat-containing protein family member 1 isoform X11
XM_025001541.1	XP_024857309.1	maestro heat-like repeat-containing protein family member 1 isoform X12
XM_025001543.1	XP_024857311.1	maestro heat-like repeat-containing protein family member 1 isoform X14
XM_025001548.1	XP_024857316.1	maestro heat-like repeat-containing protein family member 1 isoform X18
XM_010811838.2	XP_010810140.1	maestro heat-like repeat-containing protein family member 1 isoform X1
XM_025001538.1	XP_024857306.1	maestro heat-like repeat-containing protein family member 1 isoform X9
XM_005215190.4	XP_005215247.2	maestro heat-like repeat-containing protein family member 1 isoform X17
XM_025001546.1	XP_024857314.1	maestro heat-like repeat-containing protein family member 1 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	mroh1	XP_005158232.1
Drosophila melanogaster (fruit fly)	c11.1	NP_652606.1
Xenopus tropicalis (tropical clawed frog)	LOC100489995	XP_002942928.2
Pan troglodytes (chimpanzee)	LOC100609789	XP_003951430.1
	LOC713971	XP_002808538.1
Bos taurus (cattle)	MROH1	NP_001095488.1
Homo sapiens (human)	MROH1	NP_115826.2
Canis lupus familiaris (dog)	HEATR7A	XP_005628368.1
Mus musculus (house mouse)	Mroh1	NP_780666.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000273	XP_001237319.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Assessment of the gene content of the chromosomal regions flanking bovine DGAT1.
Winter A, Alzinger A, Fries R
Genomics83(1)172-80(2004 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MROH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MROH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa222284	XM_025001548.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa222269	XM_025001546.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222269	XM_025001545.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222269	XM_025001547.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222269	XM_025001529.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222270	XM_025001530.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222271	XM_025001531.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222272	XM_025001532.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222273	XM_025001533.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222274	XM_025001534.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222275	XM_025001535.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222276	XM_025001536.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222277	XM_025001538.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222278	XM_025001539.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222279	XM_025001540.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222280	XM_025001541.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222281	XM_025001542.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa222282	XM_025001543.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa222283	XM_025001544.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OBa43997	NM_001102018.1 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa49177	XM_010811839.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49177	XM_010811838.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49177	XM_010811840.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49177	XM_010811837.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49178	XM_010811841.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49179	XM_010811842.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49180	XM_010811843.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49181	XM_010811844.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49182	XM_010811845.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49183	XM_010811846.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa49184	XM_005215189.4 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa49185	XM_005215190.4 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa49186	XM_010811847.2 Latest version!	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
OBa49184	XM_005215189.3	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OBa49185	XM_005215190.3	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MROH1 ( XM_025001548.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857316.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OBa222284
Clone ID Related Accession (Same CDS sequence)	XM_025001548.1
Accession Version	XM_025001548.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4233bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857316.1
CDS	161..4393
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAE

Target ORF information:

RefSeq Version	XM_025001548.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001548.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001545.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857313.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X18, mRNA.

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CloneID	OBa222269
Clone ID Related Accession (Same CDS sequence)	XM_025001545.1 , XM_025001547.1 , XM_025001529.1 , XM_025001546.1
Accession Version	XM_025001545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857313.1
CDS	163..5355
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001545.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001545.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001547.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857315.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X20, mRNA.

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CloneID	OBa222269
Clone ID Related Accession (Same CDS sequence)	XM_025001545.1 , XM_025001547.1 , XM_025001529.1 , XM_025001546.1
Accession Version	XM_025001547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857315.1
CDS	307..5499
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001547.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001547.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001529.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857297.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.

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CloneID	OBa222269
Clone ID Related Accession (Same CDS sequence)	XM_025001545.1 , XM_025001547.1 , XM_025001529.1 , XM_025001546.1
Accession Version	XM_025001529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857297.1
CDS	161..5353
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001529.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001529.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001546.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857314.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa222269
Clone ID Related Accession (Same CDS sequence)	XM_025001545.1 , XM_025001547.1 , XM_025001529.1 , XM_025001546.1
Accession Version	XM_025001546.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5193bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857314.1
CDS	371..5563
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001546.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001546.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001530.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857298.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.

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CloneID	OBa222270
Clone ID Related Accession (Same CDS sequence)	XM_025001530.1
Accession Version	XM_025001530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GTCCCTGTAC CTGGACACCG TGCAGGTCCT CAAGGACTTG 
CTGACGAGCC TTCTTCTGTG GAACATGACG CCCCTGGGTC TGCAGGTCAT GTTGGAGCAC 
CTGAGCCCAT GGATCAAGTC CCCGAGGGGC CACGAGCGGG TGCGGGCGGT CGGCCTGAGT 
GCCTGCCTGC TGCAGTTCTT CCTTCAACAC CTGCAGATCA GCGCCCTGGT GCCCTTCCAC 
AACCTGGGCC TCCTCATTGG CCTCTTCTCC CCACGGTGCG CCGACCTCTG GCCTGCCACT 
CGCCGAGAGG CTGTGAGCTG CATCCACTCC CTGCTATACC TGCAGCTGAC CTGTGTGGGC 
TTCTCGCGAG ACTACCAGGA CGACGTGGCT GAGCAGCTCC TCAGCCTCAA AGATGGCCTG 
GTGCACCCTG ACCCTGCCAT TCTCTTCCAT ACCTGCCACA GCATTGCCCA GCTTATTGCC 
AAGCGCCTCC CTCCAGATCA GCTCATCAAC CTCTTGCTAA CCTTGTTTGA GAGCCTGGGA 
GATCCCGACA AGAACTGCTC GAGAGCTGCC AGCGTCATGA TCAACTGCCT GCTGAAGGAA 
CGGGGCAACA TGCTGCAGGA GAAGGTGCCC GAGATTGTGA GTGTGCTGCG CTCCAAGCTG 
CAGGAGACCC AAGAGGAGCA CATCCTACAG GCCGCACAGC ACAGCGTGTT TGCCCTGGCC 
ACCCACCACT GTGCCTCTGT GGTGTCCAAC CTTCTGGGCA GCCCCCTGCC CTTTGACAGC 
CACACCTGCA CCCTGTGGCG AGCACTGGCC TTGGAGCCCG GCCTCGCTGC ACAGGTCCTG 
GGGCTGCTCC TGGAGAAGAT AAGCAAGGAC GTCCTGTTTG AGGAGAGCCA GGCCTTCCTG 
CTGAGCAGCA CGCCGGACCG CGTGGCCACC CTGCTGCCCC TCGCAGCCAC CTGTGCACTG 
CACGAGGTCG CATCTGCCCC GGCGTCCGGG CCAGCAGTGC TGGAGCTCTA CCCCCAGCTG 
TTTGTGGCAC TGCTGCTGCG GGTCAGCTGC ACCGTGGGTG TCCAGCCGCC CAGGCAGCTG 
CAGGCCAAGG AGAGGAGGAG CGCCAGCTCG GGCCTGGCCT CACGGAGCCT CGAGCCTTGC 
AGCTCTGCGG TGGACGCGCT GCAGGCCGTG CTTGTCCGCG GTGGCAATGA GGATGTGGTC 
CAGTGCATGG AGCTGGACGG GGGCTGGCAG TTGCTCAGGA CCTCGGCTGG GCACGAGGAA 
GGTGTCACCC GGCTGGCCAG TGCCATGGCA AAGTTCGCAG GCCCCCGGCT GCCCCTGGTG 
ATGAAGCTGC TCTTCACCAC ACAGAGTAGC ATGTATGAGG TCCAGAGGGT CACCTCCACA 
GCCTTCCTGG CTGAGCCATG TCCCCTCAGT GACCAGGGGC CGGTGCTGCC CGGTGCACTT 
GGGCGGGTGT GGTGGTCAGC ACGCCTCTGC CCCCAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857298.1
CDS	161..5338
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001530.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001530.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GTCCCTGTAC CTGGACACCG TGCAGGTCCT CAAGGACTTG 
CTGACGAGCC TTCTTCTGTG GAACATGACG CCCCTGGGTC TGCAGGTCAT GTTGGAGCAC 
CTGAGCCCAT GGATCAAGTC CCCGAGGGGC CACGAGCGGG TGCGGGCGGT CGGCCTGAGT 
GCCTGCCTGC TGCAGTTCTT CCTTCAACAC CTGCAGATCA GCGCCCTGGT GCCCTTCCAC 
AACCTGGGCC TCCTCATTGG CCTCTTCTCC CCACGGTGCG CCGACCTCTG GCCTGCCACT 
CGCCGAGAGG CTGTGAGCTG CATCCACTCC CTGCTATACC TGCAGCTGAC CTGTGTGGGC 
TTCTCGCGAG ACTACCAGGA CGACGTGGCT GAGCAGCTCC TCAGCCTCAA AGATGGCCTG 
GTGCACCCTG ACCCTGCCAT TCTCTTCCAT ACCTGCCACA GCATTGCCCA GCTTATTGCC 
AAGCGCCTCC CTCCAGATCA GCTCATCAAC CTCTTGCTAA CCTTGTTTGA GAGCCTGGGA 
GATCCCGACA AGAACTGCTC GAGAGCTGCC AGCGTCATGA TCAACTGCCT GCTGAAGGAA 
CGGGGCAACA TGCTGCAGGA GAAGGTGCCC GAGATTGTGA GTGTGCTGCG CTCCAAGCTG 
CAGGAGACCC AAGAGGAGCA CATCCTACAG GCCGCACAGC ACAGCGTGTT TGCCCTGGCC 
ACCCACCACT GTGCCTCTGT GGTGTCCAAC CTTCTGGGCA GCCCCCTGCC CTTTGACAGC 
CACACCTGCA CCCTGTGGCG AGCACTGGCC TTGGAGCCCG GCCTCGCTGC ACAGGTCCTG 
GGGCTGCTCC TGGAGAAGAT AAGCAAGGAC GTCCTGTTTG AGGAGAGCCA GGCCTTCCTG 
CTGAGCAGCA CGCCGGACCG CGTGGCCACC CTGCTGCCCC TCGCAGCCAC CTGTGCACTG 
CACGAGGTCG CATCTGCCCC GGCGTCCGGG CCAGCAGTGC TGGAGCTCTA CCCCCAGCTG 
TTTGTGGCAC TGCTGCTGCG GGTCAGCTGC ACCGTGGGTG TCCAGCCGCC CAGGCAGCTG 
CAGGCCAAGG AGAGGAGGAG CGCCAGCTCG GGCCTGGCCT CACGGAGCCT CGAGCCTTGC 
AGCTCTGCGG TGGACGCGCT GCAGGCCGTG CTTGTCCGCG GTGGCAATGA GGATGTGGTC 
CAGTGCATGG AGCTGGACGG GGGCTGGCAG TTGCTCAGGA CCTCGGCTGG GCACGAGGAA 
GGTGTCACCC GGCTGGCCAG TGCCATGGCA AAGTTCGCAG GCCCCCGGCT GCCCCTGGTG 
ATGAAGCTGC TCTTCACCAC ACAGAGTAGC ATGTATGAGG TCCAGAGGGT CACCTCCACA 
GCCTTCCTGG CTGAGCCATG TCCCCTCAGT GACCAGGGGC CGGTGCTGCC CGGTGCACTT 
GGGCGGGTGT GGTGGTCAGC ACGCCTCTGC CCCCAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001531.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857299.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.

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CloneID	OBa222271
Clone ID Related Accession (Same CDS sequence)	XM_025001531.1
Accession Version	XM_025001531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GACCCGGCCC TGAGACTGAG CCTGGTGCAA AGTGTGTGCA TGGCCACCCA GGCCATCTGC 
AGCAGTGCCC ACAGCAGCTC CTTCCACCTC TCGAGGAAGG CGGAGCTGGT GGCGCAGATG 
GTGGAGTTCA TCAGAGCGGA GCCCCCAGAC TCGCTGAAGA CGCCCATTTG GAAGAAGGCC 
ATGCTTGCCT GCACGTACCT GGTTACCCTG GAGCCGGCCC TGGAGGAGCA GGTGCAGGCG 
GACCTGATTC ACAGCTGCCT GCACCGTGTC ATGGCCGTGC TGCCGGAGCC AGAGGAGGGG 
GACAGCCCCC AGGAGGTATC AGCGGCCCCC ACAGTGGGGG GGGACCCCCA GGAGCTTCTC 
TTGTTCCAGT CCCTGTACCT GGACACCGTG CAGGTCCTCA AGGACTTGCT GACGAGCCTT 
CTTCTGTGGA ACATGACGCC CCTGGGTCTG CAGGTCATGT TGGAGCACCT GAGCCCATGG 
ATCAAGTCCC CGAGGGGCCA CGAGCGGGTG CGGGCGGTCG GCCTGAGTGC CTGCCTGCTG 
CAGTTCTTCC TTCAACACCT GCAGATCAGC GCCCTGGTGC CCTTCCACAA CCTGGGCCTC 
CTCATTGGCC TCTTCTCCCC ACGGTGCGCC GACCTCTGGC CTGCCACTCG CCGAGAGGCT 
GTGAGCTGCA TCCACTCCCT GCTATACCTG CAGCTGACCT GTGTGGGCTT CTCGCGAGAC 
TACCAGGACG ACGTGGCTGA GCAGCTCCTC AGCCTCAAAG ATGGCCTGGT GCACCCTGAC 
CCTGCCATTC TCTTCCATAC CTGCCACAGC ATTGCCCAGC TTATTGCCAA GCGCCTCCCT 
CCAGATCAGC TCATCAACCT CTTGCTAACC TTGTTTGAGA GCCTGGGAGA TCCCGACAAG 
AACTGCTCGA GAGCTGCCAG CGTCATGATC AACTGCCTGC TGAAGGAACG GGGCAACATG 
CTGCAGGAGA AGGTGCCCGA GATTGTGAGT GTGCTGCGCT CCAAGCTGCA GGAGACCCAA 
GAGGAGCACA TCCTACAGGC CGCACAGCAC AGCGTGTTTG CCCTGGCCAC CCACCACTGT 
GCCTCTGTGG TGTCCAACCT TCTGGGCAGC CCCCTGCCCT TTGACAGCCA CACCTGCACC 
CTGTGGCGAG CACTGGCCTT GGAGCCCGGC CTCGCTGCAC AGGTCCTGGG GCTGCTCCTG 
GAGAAGATAA GCAAGGACGT CCTGTTTGAG GAGAGCCAGG CCTTCCTGCT GAGCAGCACG 
CCGGACCGCG TGGCCACCCT GCTGCCCCTC GCAGCCACCT GTGCACTGCA CGAGGTCGCA 
TCTGCCCCGG CGTCCGGGCC AGCAGTGCTG GAGCTCTACC CCCAGCTGTT TGTGGCACTG 
CTGCTGCGGG TCAGCTGCAC CGTGGGTGTC CAGCCGCCCA GGCAGCTGCA GGCCAAGGAG 
AGGAGGAGCG CCAGCTCGGG CCTGGCCTCA CGGAGCCTCG AGCCTTGCAG CTCTGCGGTG 
GACGCGCTGC AGGCCGTGCT TGTCCGCGGT GGCAATGAGG ATGTGGTCCA GTGCATGGAG 
CTGGACGGGG GCTGGCAGTT GCTCAGGACC TCGGCTGGGC ACGAGGAAGG TGTCACCCGG 
CTGGCCAGTG CCATGGCAAA GTTCGCAGGC CCCCGGCTGC CCCTGGTGAT GAAGCTGCTC 
TTCACCACAC AGAGTAGCAT GTATGAGGTC CAGAGGGTCA CCTCCACAGC CTTCCTGGCT 
GAGCCATGTC CCCTCAGTGA CCAGGGGCCG GTGCTGCCCG GTGCACTTGG GCGGGTGTGG 
TGGTCAGCAC GCCTCTGCCC CCAGCTGCTC AGCAGCAATG TGGTGAACGA CCTGATGCTC 
CTGGAGTCAC TGCTGTACAA CCTGATGGCA CGGCAGAAGG ACACGAGCGC CCGCGTGCGG 
AGGCTGGTGC TCCACGGCCT GGCCAACATC ACCTTGGGCT CCCCAGATAA GGTACAGACC 
CACAGCCCCC AACTCCTGAC AGCCATGATC GGTGGGCTGG ACGACGGGGA CGACCCACAC 
AGCCTGGTGG CGCTGGAGGC CATGGTGGGC CTGGCAAGGC TGATGGACCT GGTGGACGCC 
TGGGACCTAC ATGCAGTGCT GCTGCACATT GCTGTCCGCA TTCGGCCCTT CTTCGACAGC 
GAAAGGATGG AGTTACGCTC AGTGTCCATT GGCCTCTTCG GGCACCTCAA CAAGGCCTGC 
CGTGGGGACT GCAAGGACGT GTTCCTGGAG CAGGTTGTGG GCGGCCTGGT GCCCCTTCTG 
CTGCACCTGC GTGACCCCCA CGCACCCGTG GTCACGGCCT GCAGGTTCGC CTTGCGCATG 
TGCGGCCCCA ACCTGGAGTG TGAGGAGCTG GCAGCTGTCT TCCAGAGGCA CCTGCAGGAA 
GGCCATGACC TGCACTTTGG AGAGTTCCTC AACACCACCT GCAAGCACCT GATGCGCCAC 
TTCCCCGACC TGCTGGGCCG CTTGCTGAGC ACCAGTCTGT TCTACTATAA GAGCAGCTGG 
GAGGATGTCC GTGCTGCTGC CCCCATGCTC ACAGGGTTCT TGGTGCTGCA CATGGAGGCT 
GAGCAGCGGC CACAGGTGGA CCTGGAGCAG CTCCTCACGG CACTCCAGCT GCTGCTCAAG 
GACCCAGCCC TTAAGGTGCG CTTGAAGGCT GTGAAGACTC TGGGCCGCTT GGTGAAGTTC 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857299.1
CDS	161..5326
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001531.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001531.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GACCCGGCCC TGAGACTGAG CCTGGTGCAA AGTGTGTGCA TGGCCACCCA GGCCATCTGC 
AGCAGTGCCC ACAGCAGCTC CTTCCACCTC TCGAGGAAGG CGGAGCTGGT GGCGCAGATG 
GTGGAGTTCA TCAGAGCGGA GCCCCCAGAC TCGCTGAAGA CGCCCATTTG GAAGAAGGCC 
ATGCTTGCCT GCACGTACCT GGTTACCCTG GAGCCGGCCC TGGAGGAGCA GGTGCAGGCG 
GACCTGATTC ACAGCTGCCT GCACCGTGTC ATGGCCGTGC TGCCGGAGCC AGAGGAGGGG 
GACAGCCCCC AGGAGGTATC AGCGGCCCCC ACAGTGGGGG GGGACCCCCA GGAGCTTCTC 
TTGTTCCAGT CCCTGTACCT GGACACCGTG CAGGTCCTCA AGGACTTGCT GACGAGCCTT 
CTTCTGTGGA ACATGACGCC CCTGGGTCTG CAGGTCATGT TGGAGCACCT GAGCCCATGG 
ATCAAGTCCC CGAGGGGCCA CGAGCGGGTG CGGGCGGTCG GCCTGAGTGC CTGCCTGCTG 
CAGTTCTTCC TTCAACACCT GCAGATCAGC GCCCTGGTGC CCTTCCACAA CCTGGGCCTC 
CTCATTGGCC TCTTCTCCCC ACGGTGCGCC GACCTCTGGC CTGCCACTCG CCGAGAGGCT 
GTGAGCTGCA TCCACTCCCT GCTATACCTG CAGCTGACCT GTGTGGGCTT CTCGCGAGAC 
TACCAGGACG ACGTGGCTGA GCAGCTCCTC AGCCTCAAAG ATGGCCTGGT GCACCCTGAC 
CCTGCCATTC TCTTCCATAC CTGCCACAGC ATTGCCCAGC TTATTGCCAA GCGCCTCCCT 
CCAGATCAGC TCATCAACCT CTTGCTAACC TTGTTTGAGA GCCTGGGAGA TCCCGACAAG 
AACTGCTCGA GAGCTGCCAG CGTCATGATC AACTGCCTGC TGAAGGAACG GGGCAACATG 
CTGCAGGAGA AGGTGCCCGA GATTGTGAGT GTGCTGCGCT CCAAGCTGCA GGAGACCCAA 
GAGGAGCACA TCCTACAGGC CGCACAGCAC AGCGTGTTTG CCCTGGCCAC CCACCACTGT 
GCCTCTGTGG TGTCCAACCT TCTGGGCAGC CCCCTGCCCT TTGACAGCCA CACCTGCACC 
CTGTGGCGAG CACTGGCCTT GGAGCCCGGC CTCGCTGCAC AGGTCCTGGG GCTGCTCCTG 
GAGAAGATAA GCAAGGACGT CCTGTTTGAG GAGAGCCAGG CCTTCCTGCT GAGCAGCACG 
CCGGACCGCG TGGCCACCCT GCTGCCCCTC GCAGCCACCT GTGCACTGCA CGAGGTCGCA 
TCTGCCCCGG CGTCCGGGCC AGCAGTGCTG GAGCTCTACC CCCAGCTGTT TGTGGCACTG 
CTGCTGCGGG TCAGCTGCAC CGTGGGTGTC CAGCCGCCCA GGCAGCTGCA GGCCAAGGAG 
AGGAGGAGCG CCAGCTCGGG CCTGGCCTCA CGGAGCCTCG AGCCTTGCAG CTCTGCGGTG 
GACGCGCTGC AGGCCGTGCT TGTCCGCGGT GGCAATGAGG ATGTGGTCCA GTGCATGGAG 
CTGGACGGGG GCTGGCAGTT GCTCAGGACC TCGGCTGGGC ACGAGGAAGG TGTCACCCGG 
CTGGCCAGTG CCATGGCAAA GTTCGCAGGC CCCCGGCTGC CCCTGGTGAT GAAGCTGCTC 
TTCACCACAC AGAGTAGCAT GTATGAGGTC CAGAGGGTCA CCTCCACAGC CTTCCTGGCT 
GAGCCATGTC CCCTCAGTGA CCAGGGGCCG GTGCTGCCCG GTGCACTTGG GCGGGTGTGG 
TGGTCAGCAC GCCTCTGCCC CCAGCTGCTC AGCAGCAATG TGGTGAACGA CCTGATGCTC 
CTGGAGTCAC TGCTGTACAA CCTGATGGCA CGGCAGAAGG ACACGAGCGC CCGCGTGCGG 
AGGCTGGTGC TCCACGGCCT GGCCAACATC ACCTTGGGCT CCCCAGATAA GGTACAGACC 
CACAGCCCCC AACTCCTGAC AGCCATGATC GGTGGGCTGG ACGACGGGGA CGACCCACAC 
AGCCTGGTGG CGCTGGAGGC CATGGTGGGC CTGGCAAGGC TGATGGACCT GGTGGACGCC 
TGGGACCTAC ATGCAGTGCT GCTGCACATT GCTGTCCGCA TTCGGCCCTT CTTCGACAGC 
GAAAGGATGG AGTTACGCTC AGTGTCCATT GGCCTCTTCG GGCACCTCAA CAAGGCCTGC 
CGTGGGGACT GCAAGGACGT GTTCCTGGAG CAGGTTGTGG GCGGCCTGGT GCCCCTTCTG 
CTGCACCTGC GTGACCCCCA CGCACCCGTG GTCACGGCCT GCAGGTTCGC CTTGCGCATG 
TGCGGCCCCA ACCTGGAGTG TGAGGAGCTG GCAGCTGTCT TCCAGAGGCA CCTGCAGGAA 
GGCCATGACC TGCACTTTGG AGAGTTCCTC AACACCACCT GCAAGCACCT GATGCGCCAC 
TTCCCCGACC TGCTGGGCCG CTTGCTGAGC ACCAGTCTGT TCTACTATAA GAGCAGCTGG 
GAGGATGTCC GTGCTGCTGC CCCCATGCTC ACAGGGTTCT TGGTGCTGCA CATGGAGGCT 
GAGCAGCGGC CACAGGTGGA CCTGGAGCAG CTCCTCACGG CACTCCAGCT GCTGCTCAAG 
GACCCAGCCC TTAAGGTGCG CTTGAAGGCT GTGAAGACTC TGGGCCGCTT GGTGAAGTTC 
GCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001532.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857300.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.

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CloneID	OBa222272
Clone ID Related Accession (Same CDS sequence)	XM_025001532.1
Accession Version	XM_025001532.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5154bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGCTTCTCTT GTTCCAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCCATGTCCC 
CTCAGTGACC AGGGGCCGGT GCTGCCCGGT GCACTTGGGC GGGTGTGGTG GTCAGCACGC 
CTCTGCCCCC AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857300.1
CDS	161..5314
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001532.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001532.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGCTTCTCTT GTTCCAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCCATGTCCC 
CTCAGTGACC AGGGGCCGGT GCTGCCCGGT GCACTTGGGC GGGTGTGGTG GTCAGCACGC 
CTCTGCCCCC AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001533.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857301.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.

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CloneID	OBa222273
Clone ID Related Accession (Same CDS sequence)	XM_025001533.1
Accession Version	XM_025001533.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCCAT GTCCCCTCAG TGACCAGGGG 
CCGGTGCTGC CCGGTGCACT TGGGCGGGTG TGGTGGTCAG CACGCCTCTG CCCCCAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857301.1
CDS	161..5299
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001533.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001533.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCCAT GTCCCCTCAG TGACCAGGGG 
CCGGTGCTGC CCGGTGCACT TGGGCGGGTG TGGTGGTCAG CACGCCTCTG CCCCCAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001534.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857302.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.

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CloneID	OBa222274
Clone ID Related Accession (Same CDS sequence)	XM_025001534.1
Accession Version	XM_025001534.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGTTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857302.1
CDS	161..5293
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001534.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001534.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGTTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CCATGTCCCC TCAGTGACCA GGGGCCGGTG 
CTGCCCGGTG CACTTGGGCG GGTGTGGTGG TCAGCACGCC TCTGCCCCCA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001535.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857303.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.

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CloneID	OBa222275
Clone ID Related Accession (Same CDS sequence)	XM_025001535.1
Accession Version	XM_025001535.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5115bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGCTTCTCT TGTTCCAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCCATGTCC CCTCAGTGAC CAGGGGCCGG TGCTGCCCGG TGCACTTGGG 
CGGGTGTGGT GGTCAGCACG CCTCTGCCCC CAGCTGCTCA GCAGCAATGT GGTGAACGAC 
CTGATGCTCC TGGAGTCACT GCTGTACAAC CTGATGGCAC GGCAGAAGGA CACGAGCGCC 
CGCGTGCGGA GGCTGGTGCT CCACGGCCTG GCCAACATCA CCTTGGGCTC CCCAGATAAG 
GTACAGACCC ACAGCCCCCA ACTCCTGACA GCCATGATCG GTGGGCTGGA CGACGGGGAC 
GACCCACACA GCCTGGTGGC GCTGGAGGCC ATGGTGGGCC TGGCAAGGCT GATGGACCTG 
GTGGACGCCT GGGACCTACA TGCAGTGCTG CTGCACATTG CTGTCCGCAT TCGGCCCTTC 
TTCGACAGCG AAAGGATGGA GTTACGCTCA GTGTCCATTG GCCTCTTCGG GCACCTCAAC 
AAGGCCTGCC GTGGGGACTG CAAGGACGTG TTCCTGGAGC AGGTTGTGGG CGGCCTGGTG 
CCCCTTCTGC TGCACCTGCG TGACCCCCAC GCACCCGTGG TCACGGCCTG CAGGTTCGCC 
TTGCGCATGT GCGGCCCCAA CCTGGAGTGT GAGGAGCTGG CAGCTGTCTT CCAGAGGCAC 
CTGCAGGAAG GCCATGACCT GCACTTTGGA GAGTTCCTCA ACACCACCTG CAAGCACCTG 
ATGCGCCACT TCCCCGACCT GCTGGGCCGC TTGCTGAGCA CCAGTCTGTT CTACTATAAG 
AGCAGCTGGG AGGATGTCCG TGCTGCTGCC CCCATGCTCA CAGGGTTCTT GGTGCTGCAC 
ATGGAGGCTG AGCAGCGGCC ACAGGTGGAC CTGGAGCAGC TCCTCACGGC ACTCCAGCTG 
CTGCTCAAGG ACCCAGCCCT TAAGGTGCGC TTGAAGGCTG TGAAGACTCT GGGCCGCTTG 
GTGAAGTTCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857303.1
CDS	161..5275
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001535.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001535.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGCTTCTCT TGTTCCAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCCATGTCC CCTCAGTGAC CAGGGGCCGG TGCTGCCCGG TGCACTTGGG 
CGGGTGTGGT GGTCAGCACG CCTCTGCCCC CAGCTGCTCA GCAGCAATGT GGTGAACGAC 
CTGATGCTCC TGGAGTCACT GCTGTACAAC CTGATGGCAC GGCAGAAGGA CACGAGCGCC 
CGCGTGCGGA GGCTGGTGCT CCACGGCCTG GCCAACATCA CCTTGGGCTC CCCAGATAAG 
GTACAGACCC ACAGCCCCCA ACTCCTGACA GCCATGATCG GTGGGCTGGA CGACGGGGAC 
GACCCACACA GCCTGGTGGC GCTGGAGGCC ATGGTGGGCC TGGCAAGGCT GATGGACCTG 
GTGGACGCCT GGGACCTACA TGCAGTGCTG CTGCACATTG CTGTCCGCAT TCGGCCCTTC 
TTCGACAGCG AAAGGATGGA GTTACGCTCA GTGTCCATTG GCCTCTTCGG GCACCTCAAC 
AAGGCCTGCC GTGGGGACTG CAAGGACGTG TTCCTGGAGC AGGTTGTGGG CGGCCTGGTG 
CCCCTTCTGC TGCACCTGCG TGACCCCCAC GCACCCGTGG TCACGGCCTG CAGGTTCGCC 
TTGCGCATGT GCGGCCCCAA CCTGGAGTGT GAGGAGCTGG CAGCTGTCTT CCAGAGGCAC 
CTGCAGGAAG GCCATGACCT GCACTTTGGA GAGTTCCTCA ACACCACCTG CAAGCACCTG 
ATGCGCCACT TCCCCGACCT GCTGGGCCGC TTGCTGAGCA CCAGTCTGTT CTACTATAAG 
AGCAGCTGGG AGGATGTCCG TGCTGCTGCC CCCATGCTCA CAGGGTTCTT GGTGCTGCAC 
ATGGAGGCTG AGCAGCGGCC ACAGGTGGAC CTGGAGCAGC TCCTCACGGC ACTCCAGCTG 
CTGCTCAAGG ACCCAGCCCT TAAGGTGCGC TTGAAGGCTG TGAAGACTCT GGGCCGCTTG 
GTGAAGTTCG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001536.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857304.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OBa222276
Clone ID Related Accession (Same CDS sequence)	XM_025001536.1
Accession Version	XM_025001536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CTGCTCAGCA GCAATGTGGT GAACGACCTG 
ATGCTCCTGG AGTCACTGCT GTACAACCTG ATGGCACGGC AGAAGGACAC GAGCGCCCGC 
GTGCGGAGGC TGGTGCTCCA CGGCCTGGCC AACATCACCT TGGGCTCCCC AGATAAGGTA 
CAGACCCACA GCCCCCAACT CCTGACAGCC ATGATCGGTG GGCTGGACGA CGGGGACGAC 
CCACACAGCC TGGTGGCGCT GGAGGCCATG GTGGGCCTGG CAAGGCTGAT GGACCTGGTG 
GACGCCTGGG ACCTACATGC AGTGCTGCTG CACATTGCTG TCCGCATTCG GCCCTTCTTC 
GACAGCGAAA GGATGGAGTT ACGCTCAGTG TCCATTGGCC TCTTCGGGCA CCTCAACAAG 
GCCTGCCGTG GGGACTGCAA GGACGTGTTC CTGGAGCAGG TTGTGGGCGG CCTGGTGCCC 
CTTCTGCTGC ACCTGCGTGA CCCCCACGCA CCCGTGGTCA CGGCCTGCAG GTTCGCCTTG 
CGCATGTGCG GCCCCAACCT GGAGTGTGAG GAGCTGGCAG CTGTCTTCCA GAGGCACCTG 
CAGGAAGGCC ATGACCTGCA CTTTGGAGAG TTCCTCAACA CCACCTGCAA GCACCTGATG 
CGCCACTTCC CCGACCTGCT GGGCCGCTTG CTGAGCACCA GTCTGTTCTA CTATAAGAGC 
AGCTGGGAGG ATGTCCGTGC TGCTGCCCCC ATGCTCACAG GGTTCTTGGT GCTGCACATG 
GAGGCTGAGC AGCGGCCACA GGTGGACCTG GAGCAGCTCC TCACGGCACT CCAGCTGCTG 
CTCAAGGACC CAGCCCTTAA GGTGCGCTTG AAGGCTGTGA AGACTCTGGG CCGCTTGGTG 
AAGTTCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857304.1
CDS	161..5272
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001536.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001536.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GCTTCTCTTG TTCCAGTCCC TGTACCTGGA CACCGTGCAG 
GTCCTCAAGG ACTTGCTGAC GAGCCTTCTT CTGTGGAACA TGACGCCCCT GGGTCTGCAG 
GTCATGTTGG AGCACCTGAG CCCATGGATC AAGTCCCCGA GGGGCCACGA GCGGGTGCGG 
GCGGTCGGCC TGAGTGCCTG CCTGCTGCAG TTCTTCCTTC AACACCTGCA GATCAGCGCC 
CTGGTGCCCT TCCACAACCT GGGCCTCCTC ATTGGCCTCT TCTCCCCACG GTGCGCCGAC 
CTCTGGCCTG CCACTCGCCG AGAGGCTGTG AGCTGCATCC ACTCCCTGCT ATACCTGCAG 
CTGACCTGTG TGGGCTTCTC GCGAGACTAC CAGGACGACG TGGCTGAGCA GCTCCTCAGC 
CTCAAAGATG GCCTGGTGCA CCCTGACCCT GCCATTCTCT TCCATACCTG CCACAGCATT 
GCCCAGCTTA TTGCCAAGCG CCTCCCTCCA GATCAGCTCA TCAACCTCTT GCTAACCTTG 
TTTGAGAGCC TGGGAGATCC CGACAAGAAC TGCTCGAGAG CTGCCAGCGT CATGATCAAC 
TGCCTGCTGA AGGAACGGGG CAACATGCTG CAGGAGAAGG TGCCCGAGAT TGTGAGTGTG 
CTGCGCTCCA AGCTGCAGGA GACCCAAGAG GAGCACATCC TACAGGCCGC ACAGCACAGC 
GTGTTTGCCC TGGCCACCCA CCACTGTGCC TCTGTGGTGT CCAACCTTCT GGGCAGCCCC 
CTGCCCTTTG ACAGCCACAC CTGCACCCTG TGGCGAGCAC TGGCCTTGGA GCCCGGCCTC 
GCTGCACAGG TCCTGGGGCT GCTCCTGGAG AAGATAAGCA AGGACGTCCT GTTTGAGGAG 
AGCCAGGCCT TCCTGCTGAG CAGCACGCCG GACCGCGTGG CCACCCTGCT GCCCCTCGCA 
GCCACCTGTG CACTGCACGA GGTCGCATCT GCCCCGGCGT CCGGGCCAGC AGTGCTGGAG 
CTCTACCCCC AGCTGTTTGT GGCACTGCTG CTGCGGGTCA GCTGCACCGT GGGTGTCCAG 
CCGCCCAGGC AGCTGCAGGC CAAGGAGAGG AGGAGCGCCA GCTCGGGCCT GGCCTCACGG 
AGCCTCGAGC CTTGCAGCTC TGCGGTGGAC GCGCTGCAGG CCGTGCTTGT CCGCGGTGGC 
AATGAGGATG TGGTCCAGTG CATGGAGCTG GACGGGGGCT GGCAGTTGCT CAGGACCTCG 
GCTGGGCACG AGGAAGGTGT CACCCGGCTG GCCAGTGCCA TGGCAAAGTT CGCAGGCCCC 
CGGCTGCCCC TGGTGATGAA GCTGCTCTTC ACCACACAGA GTAGCATGTA TGAGGTCCAG 
AGGGTCACCT CCACAGCCTT CCTGGCTGAG CTGCTCAGCA GCAATGTGGT GAACGACCTG 
ATGCTCCTGG AGTCACTGCT GTACAACCTG ATGGCACGGC AGAAGGACAC GAGCGCCCGC 
GTGCGGAGGC TGGTGCTCCA CGGCCTGGCC AACATCACCT TGGGCTCCCC AGATAAGGTA 
CAGACCCACA GCCCCCAACT CCTGACAGCC ATGATCGGTG GGCTGGACGA CGGGGACGAC 
CCACACAGCC TGGTGGCGCT GGAGGCCATG GTGGGCCTGG CAAGGCTGAT GGACCTGGTG 
GACGCCTGGG ACCTACATGC AGTGCTGCTG CACATTGCTG TCCGCATTCG GCCCTTCTTC 
GACAGCGAAA GGATGGAGTT ACGCTCAGTG TCCATTGGCC TCTTCGGGCA CCTCAACAAG 
GCCTGCCGTG GGGACTGCAA GGACGTGTTC CTGGAGCAGG TTGTGGGCGG CCTGGTGCCC 
CTTCTGCTGC ACCTGCGTGA CCCCCACGCA CCCGTGGTCA CGGCCTGCAG GTTCGCCTTG 
CGCATGTGCG GCCCCAACCT GGAGTGTGAG GAGCTGGCAG CTGTCTTCCA GAGGCACCTG 
CAGGAAGGCC ATGACCTGCA CTTTGGAGAG TTCCTCAACA CCACCTGCAA GCACCTGATG 
CGCCACTTCC CCGACCTGCT GGGCCGCTTG CTGAGCACCA GTCTGTTCTA CTATAAGAGC 
AGCTGGGAGG ATGTCCGTGC TGCTGCCCCC ATGCTCACAG GGTTCTTGGT GCTGCACATG 
GAGGCTGAGC AGCGGCCACA GGTGGACCTG GAGCAGCTCC TCACGGCACT CCAGCTGCTG 
CTCAAGGACC CAGCCCTTAA GGTGCGCTTG AAGGCTGTGA AGACTCTGGG CCGCTTGGTG 
AAGTTCGCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001538.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857306.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa222277
Clone ID Related Accession (Same CDS sequence)	XM_025001538.1
Accession Version	XM_025001538.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GTCCCTGTAC CTGGACACCG TGCAGGTCCT CAAGGACTTG 
CTGACGAGCC TTCTTCTGTG GAACATGACG CCCCTGGGTC TGCAGGTCAT GTTGGAGCAC 
CTGAGCCCAT GGATCAAGTC CCCGAGGGGC CACGAGCGGG TGCGGGCGGT CGGCCTGAGT 
GCCTGCCTGC TGCAGTTCTT CCTTCAACAC CTGCAGATCA GCGCCCTGGT GCCCTTCCAC 
AACCTGGGCC TCCTCATTGG CCTCTTCTCC CCACGGTGCG CCGACCTCTG GCCTGCCACT 
CGCCGAGAGG CTGTGAGCTG CATCCACTCC CTGCTATACC TGCAGCTGAC CTGTGTGGGC 
TTCTCGCGAG ACTACCAGGA CGACGTGGCT GAGCAGCTCC TCAGCCTCAA AGATGGCCTG 
GTGCACCCTG ACCCTGCCAT TCTCTTCCAT ACCTGCCACA GCATTGCCCA GCTTATTGCC 
AAGCGCCTCC CTCCAGATCA GCTCATCAAC CTCTTGCTAA CCTTGTTTGA GAGCCTGGGA 
GATCCCGACA AGAACTGCTC GAGAGCTGCC AGCGTCATGA TCAACTGCCT GCTGAAGGAA 
CGGGGCAACA TGCTGCAGGA GAAGGTGCCC GAGATTGTGA GTGTGCTGCG CTCCAAGCTG 
CAGGAGACCC AAGAGGAGCA CATCCTACAG GCCGCACAGC ACAGCGTGTT TGCCCTGGCC 
ACCCACCACT GTGCCTCTGT GGTGTCCAAC CTTCTGGGCA GCCCCCTGCC CTTTGACAGC 
CACACCTGCA CCCTGTGGCG AGCACTGGCC TTGGAGCCCG GCCTCGCTGC ACAGGTCCTG 
GGGCTGCTCC TGGAGAAGAT AAGCAAGGAC GTCCTGTTTG AGGAGAGCCA GGCCTTCCTG 
CTGAGCAGCA CGCCGGACCG CGTGGCCACC CTGCTGCCCC TCGCAGCCAC CTGTGCACTG 
CACGAGGTCG CATCTGCCCC GGCGTCCGGG CCAGCAGTGC TGGAGCTCTA CCCCCAGCTG 
TTTGTGGCAC TGCTGCTGCG GGTCAGCTGC ACCGTGGGTG TCCAGCCGCC CAGGCAGCTG 
CAGGCCAAGG AGAGGAGGAG CGCCAGCTCG GGCCTGGCCT CACGGAGCCT CGAGCCTTGC 
AGCTCTGCGG TGGACGCGCT GCAGGCCGTG CTTGTCCGCG GTGGCAATGA GGATGTGGTC 
CAGTGCATGG AGCTGGACGG GGGCTGGCAG TTGCTCAGGA CCTCGGCTGG GCACGAGGAA 
GGTGTCACCC GGCTGGCCAG TGCCATGGCA AAGTTCGCAG GCCCCCGGCT GCCCCTGGTG 
ATGAAGCTGC TCTTCACCAC ACAGAGTAGC ATGTATGAGG TCCAGAGGGT CACCTCCACA 
GCCTTCCTGG CTGAGCTGCT CAGCAGCAAT GTGGTGAACG ACCTGATGCT CCTGGAGTCA 
CTGCTGTACA ACCTGATGGC ACGGCAGAAG GACACGAGCG CCCGCGTGCG GAGGCTGGTG 
CTCCACGGCC TGGCCAACAT CACCTTGGGC TCCCCAGATA AGGTACAGAC CCACAGCCCC 
CAACTCCTGA CAGCCATGAT CGGTGGGCTG GACGACGGGG ACGACCCACA CAGCCTGGTG 
GCGCTGGAGG CCATGGTGGG CCTGGCAAGG CTGATGGACC TGGTGGACGC CTGGGACCTA 
CATGCAGTGC TGCTGCACAT TGCTGTCCGC ATTCGGCCCT TCTTCGACAG CGAAAGGATG 
GAGTTACGCT CAGTGTCCAT TGGCCTCTTC GGGCACCTCA ACAAGGCCTG CCGTGGGGAC 
TGCAAGGACG TGTTCCTGGA GCAGGTTGTG GGCGGCCTGG TGCCCCTTCT GCTGCACCTG 
CGTGACCCCC ACGCACCCGT GGTCACGGCC TGCAGGTTCG CCTTGCGCAT GTGCGGCCCC 
AACCTGGAGT GTGAGGAGCT GGCAGCTGTC TTCCAGAGGC ACCTGCAGGA AGGCCATGAC 
CTGCACTTTG GAGAGTTCCT CAACACCACC TGCAAGCACC TGATGCGCCA CTTCCCCGAC 
CTGCTGGGCC GCTTGCTGAG CACCAGTCTG TTCTACTATA AGAGCAGCTG GGAGGATGTC 
CGTGCTGCTG CCCCCATGCT CACAGGGTTC TTGGTGCTGC ACATGGAGGC TGAGCAGCGG 
CCACAGGTGG ACCTGGAGCA GCTCCTCACG GCACTCCAGC TGCTGCTCAA GGACCCAGCC 
CTTAAGGTGC GCTTGAAGGC TGTGAAGACT CTGGGCCGCT TGGTGAAGTT CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857306.1
CDS	161..5257
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001538.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001538.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGGTATCAGC GGCCCCCACA 
GTGGGGGGGG ACCCCCAGGA GTCCCTGTAC CTGGACACCG TGCAGGTCCT CAAGGACTTG 
CTGACGAGCC TTCTTCTGTG GAACATGACG CCCCTGGGTC TGCAGGTCAT GTTGGAGCAC 
CTGAGCCCAT GGATCAAGTC CCCGAGGGGC CACGAGCGGG TGCGGGCGGT CGGCCTGAGT 
GCCTGCCTGC TGCAGTTCTT CCTTCAACAC CTGCAGATCA GCGCCCTGGT GCCCTTCCAC 
AACCTGGGCC TCCTCATTGG CCTCTTCTCC CCACGGTGCG CCGACCTCTG GCCTGCCACT 
CGCCGAGAGG CTGTGAGCTG CATCCACTCC CTGCTATACC TGCAGCTGAC CTGTGTGGGC 
TTCTCGCGAG ACTACCAGGA CGACGTGGCT GAGCAGCTCC TCAGCCTCAA AGATGGCCTG 
GTGCACCCTG ACCCTGCCAT TCTCTTCCAT ACCTGCCACA GCATTGCCCA GCTTATTGCC 
AAGCGCCTCC CTCCAGATCA GCTCATCAAC CTCTTGCTAA CCTTGTTTGA GAGCCTGGGA 
GATCCCGACA AGAACTGCTC GAGAGCTGCC AGCGTCATGA TCAACTGCCT GCTGAAGGAA 
CGGGGCAACA TGCTGCAGGA GAAGGTGCCC GAGATTGTGA GTGTGCTGCG CTCCAAGCTG 
CAGGAGACCC AAGAGGAGCA CATCCTACAG GCCGCACAGC ACAGCGTGTT TGCCCTGGCC 
ACCCACCACT GTGCCTCTGT GGTGTCCAAC CTTCTGGGCA GCCCCCTGCC CTTTGACAGC 
CACACCTGCA CCCTGTGGCG AGCACTGGCC TTGGAGCCCG GCCTCGCTGC ACAGGTCCTG 
GGGCTGCTCC TGGAGAAGAT AAGCAAGGAC GTCCTGTTTG AGGAGAGCCA GGCCTTCCTG 
CTGAGCAGCA CGCCGGACCG CGTGGCCACC CTGCTGCCCC TCGCAGCCAC CTGTGCACTG 
CACGAGGTCG CATCTGCCCC GGCGTCCGGG CCAGCAGTGC TGGAGCTCTA CCCCCAGCTG 
TTTGTGGCAC TGCTGCTGCG GGTCAGCTGC ACCGTGGGTG TCCAGCCGCC CAGGCAGCTG 
CAGGCCAAGG AGAGGAGGAG CGCCAGCTCG GGCCTGGCCT CACGGAGCCT CGAGCCTTGC 
AGCTCTGCGG TGGACGCGCT GCAGGCCGTG CTTGTCCGCG GTGGCAATGA GGATGTGGTC 
CAGTGCATGG AGCTGGACGG GGGCTGGCAG TTGCTCAGGA CCTCGGCTGG GCACGAGGAA 
GGTGTCACCC GGCTGGCCAG TGCCATGGCA AAGTTCGCAG GCCCCCGGCT GCCCCTGGTG 
ATGAAGCTGC TCTTCACCAC ACAGAGTAGC ATGTATGAGG TCCAGAGGGT CACCTCCACA 
GCCTTCCTGG CTGAGCTGCT CAGCAGCAAT GTGGTGAACG ACCTGATGCT CCTGGAGTCA 
CTGCTGTACA ACCTGATGGC ACGGCAGAAG GACACGAGCG CCCGCGTGCG GAGGCTGGTG 
CTCCACGGCC TGGCCAACAT CACCTTGGGC TCCCCAGATA AGGTACAGAC CCACAGCCCC 
CAACTCCTGA CAGCCATGAT CGGTGGGCTG GACGACGGGG ACGACCCACA CAGCCTGGTG 
GCGCTGGAGG CCATGGTGGG CCTGGCAAGG CTGATGGACC TGGTGGACGC CTGGGACCTA 
CATGCAGTGC TGCTGCACAT TGCTGTCCGC ATTCGGCCCT TCTTCGACAG CGAAAGGATG 
GAGTTACGCT CAGTGTCCAT TGGCCTCTTC GGGCACCTCA ACAAGGCCTG CCGTGGGGAC 
TGCAAGGACG TGTTCCTGGA GCAGGTTGTG GGCGGCCTGG TGCCCCTTCT GCTGCACCTG 
CGTGACCCCC ACGCACCCGT GGTCACGGCC TGCAGGTTCG CCTTGCGCAT GTGCGGCCCC 
AACCTGGAGT GTGAGGAGCT GGCAGCTGTC TTCCAGAGGC ACCTGCAGGA AGGCCATGAC 
CTGCACTTTG GAGAGTTCCT CAACACCACC TGCAAGCACC TGATGCGCCA CTTCCCCGAC 
CTGCTGGGCC GCTTGCTGAG CACCAGTCTG TTCTACTATA AGAGCAGCTG GGAGGATGTC 
CGTGCTGCTG CCCCCATGCT CACAGGGTTC TTGGTGCTGC ACATGGAGGC TGAGCAGCGG 
CCACAGGTGG ACCTGGAGCA GCTCCTCACG GCACTCCAGC TGCTGCTCAA GGACCCAGCC 
CTTAAGGTGC GCTTGAAGGC TGTGAAGACT CTGGGCCGCT TGGTGAAGTT CGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001539.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857307.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.

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CloneID	OBa222278
Clone ID Related Accession (Same CDS sequence)	XM_025001539.1
Accession Version	XM_025001539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG AATCGAAGTG AGAGTGAGGC CAACAAAGTG 
AGGAGTGCGC TGATCCTGTG CTATGGGCAT GTGGCGGCCG GGGCCCCGCG CGAGCTGCTG 
CTGGCCAGGG TGGAGGCGGA CTTGTTCTGG AACATGTCCG AGTGCTTCAG CACCAAGGTT 
CTGGGGATAA AGGTAGAAAC CCAGGACCCG GCCCTGAGAC TGAGCCTGGT GCAAAGTGTG 
TGCATGGCCA CCCAGGCCAT CTGCAGCAGT GCCCACAGCA GCTCCTTCCA CCTCTCGAGG 
AAGGCGGAGC TGGTGGCGCA GATGGTGGAG TTCATCAGAG CGGAGCCCCC AGACTCGCTG 
AAGACGCCCA TTTGGAAGAA GGCCATGCTT GCCTGCACGT ACCTGGTTAC CCTGGAGCCG 
GCCCTGGAGG AGCAGGTGCA GGCGGACCTG ATTCACAGCT GCCTGCACCG TGTCATGGCC 
GTGCTGCCGG AGCCAGAGGA GGGGGACAGC CCCCAGGAGG TATCAGCGGC CCCCACAGTG 
GGGGGGGACC CCCAGGAGCT TCTCTTGTTC CAGTCCCTGT ACCTGGACAC CGTGCAGGTC 
CTCAAGGACT TGCTGACGAG CCTTCTTCTG TGGAACATGA CGCCCCTGGG TCTGCAGGTC 
ATGTTGGAGC ACCTGAGCCC ATGGATCAAG TCCCCGAGGG GCCACGAGCG GGTGCGGGCG 
GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG 
GTGCCCTTCC ACAACCTGGG CCTCCTCATT GGCCTCTTCT CCCCACGGTG CGCCGACCTC 
TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC TGCATCCACT CCCTGCTATA CCTGCAGCTG 
ACCTGTGTGG GCTTCTCGCG AGACTACCAG GACGACGTGG CTGAGCAGCT CCTCAGCCTC 
AAAGATGGCC TGGTGCACCC TGACCCTGCC ATTCTCTTCC ATACCTGCCA CAGCATTGCC 
CAGCTTATTG CCAAGCGCCT CCCTCCAGAT CAGCTCATCA ACCTCTTGCT AACCTTGTTT 
GAGAGCCTGG GAGATCCCGA CAAGAACTGC TCGAGAGCTG CCAGCGTCAT GATCAACTGC 
CTGCTGAAGG AACGGGGCAA CATGCTGCAG GAGAAGGTGC CCGAGATTGT GAGTGTGCTG 
CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG CACATCCTAC AGGCCGCACA GCACAGCGTG 
TTTGCCCTGG CCACCCACCA CTGTGCCTCT GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG 
CCCTTTGACA GCCACACCTG CACCCTGTGG CGAGCACTGG CCTTGGAGCC CGGCCTCGCT 
GCACAGGTCC TGGGGCTGCT CCTGGAGAAG ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC 
CAGGCCTTCC TGCTGAGCAG CACGCCGGAC CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC 
ACCTGTGCAC TGCACGAGGT CGCATCTGCC CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC 
TACCCCCAGC TGTTTGTGGC ACTGCTGCTG CGGGTCAGCT GCACCGTGGG TGTCCAGCCG 
CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG AGCGCCAGCT CGGGCCTGGC CTCACGGAGC 
CTCGAGCCTT GCAGCTCTGC GGTGGACGCG CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT 
GAGGATGTGG TCCAGTGCAT GGAGCTGGAC GGGGGCTGGC AGTTGCTCAG GACCTCGGCT 
GGGCACGAGG AAGGTGTCAC CCGGCTGGCC AGTGCCATGG CAAAGTTCGC AGGCCCCCGG 
CTGCCCCTGG TGATGAAGCT GCTCTTCACC ACACAGAGTA GCATGTATGA GGTCCAGAGG 
GTCACCTCCA CAGCCTTCCT GGCTGAGCCA TGTCCCCTCA GTGACCAGGG GCCGGTGCTG 
CCCGGTGCAC TTGGGCGGGT GTGGTGGTCA GCACGCCTCT GCCCCCAGCT GCTCAGCAGC 
AATGTGGTGA ACGACCTGAT GCTCCTGGAG TCACTGCTGT ACAACCTGAT GGCACGGCAG 
AAGGACACGA GCGCCCGCGT GCGGAGGCTG GTGCTCCACG GCCTGGCCAA CATCACCTTG 
GGCTCCCCAG ATAAGGTACA GACCCACAGC CCCCAACTCC TGACAGCCAT GATCGGTGGG 
CTGGACGACG GGGACGACCC ACACAGCCTG GTGGCGCTGG AGGCCATGGT GGGCCTGGCA 
AGGCTGATGG ACCTGGTGGA CGCCTGGGAC CTACATGCAG TGCTGCTGCA CATTGCTGTC 
CGCATTCGGC CCTTCTTCGA CAGCGAAAGG ATGGAGTTAC GCTCAGTGTC CATTGGCCTC 
TTCGGGCACC TCAACAAGGC CTGCCGTGGG GACTGCAAGG ACGTGTTCCT GGAGCAGGTT 
GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC CTGCGTGACC CCCACGCACC CGTGGTCACG 
GCCTGCAGGT TCGCCTTGCG CATGTGCGGC CCCAACCTGG AGTGTGAGGA GCTGGCAGCT 
GTCTTCCAGA GGCACCTGCA GGAAGGCCAT GACCTGCACT TTGGAGAGTT CCTCAACACC 
ACCTGCAAGC ACCTGATGCG CCACTTCCCC GACCTGCTGG GCCGCTTGCT GAGCACCAGT 
CTGTTCTACT ATAAGAGCAG CTGGGAGGAT GTCCGTGCTG CTGCCCCCAT GCTCACAGGG 
TTCTTGGTGC TGCACATGGA GGCTGAGCAG CGGCCACAGG TGGACCTGGA GCAGCTCCTC 
ACGGCACTCC AGCTGCTGCT CAAGGACCCA GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG 
ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857307.1
CDS	161..5230
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHENRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001539.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001539.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG AATCGAAGTG AGAGTGAGGC CAACAAAGTG 
AGGAGTGCGC TGATCCTGTG CTATGGGCAT GTGGCGGCCG GGGCCCCGCG CGAGCTGCTG 
CTGGCCAGGG TGGAGGCGGA CTTGTTCTGG AACATGTCCG AGTGCTTCAG CACCAAGGTT 
CTGGGGATAA AGGTAGAAAC CCAGGACCCG GCCCTGAGAC TGAGCCTGGT GCAAAGTGTG 
TGCATGGCCA CCCAGGCCAT CTGCAGCAGT GCCCACAGCA GCTCCTTCCA CCTCTCGAGG 
AAGGCGGAGC TGGTGGCGCA GATGGTGGAG TTCATCAGAG CGGAGCCCCC AGACTCGCTG 
AAGACGCCCA TTTGGAAGAA GGCCATGCTT GCCTGCACGT ACCTGGTTAC CCTGGAGCCG 
GCCCTGGAGG AGCAGGTGCA GGCGGACCTG ATTCACAGCT GCCTGCACCG TGTCATGGCC 
GTGCTGCCGG AGCCAGAGGA GGGGGACAGC CCCCAGGAGG TATCAGCGGC CCCCACAGTG 
GGGGGGGACC CCCAGGAGCT TCTCTTGTTC CAGTCCCTGT ACCTGGACAC CGTGCAGGTC 
CTCAAGGACT TGCTGACGAG CCTTCTTCTG TGGAACATGA CGCCCCTGGG TCTGCAGGTC 
ATGTTGGAGC ACCTGAGCCC ATGGATCAAG TCCCCGAGGG GCCACGAGCG GGTGCGGGCG 
GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG 
GTGCCCTTCC ACAACCTGGG CCTCCTCATT GGCCTCTTCT CCCCACGGTG CGCCGACCTC 
TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC TGCATCCACT CCCTGCTATA CCTGCAGCTG 
ACCTGTGTGG GCTTCTCGCG AGACTACCAG GACGACGTGG CTGAGCAGCT CCTCAGCCTC 
AAAGATGGCC TGGTGCACCC TGACCCTGCC ATTCTCTTCC ATACCTGCCA CAGCATTGCC 
CAGCTTATTG CCAAGCGCCT CCCTCCAGAT CAGCTCATCA ACCTCTTGCT AACCTTGTTT 
GAGAGCCTGG GAGATCCCGA CAAGAACTGC TCGAGAGCTG CCAGCGTCAT GATCAACTGC 
CTGCTGAAGG AACGGGGCAA CATGCTGCAG GAGAAGGTGC CCGAGATTGT GAGTGTGCTG 
CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG CACATCCTAC AGGCCGCACA GCACAGCGTG 
TTTGCCCTGG CCACCCACCA CTGTGCCTCT GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG 
CCCTTTGACA GCCACACCTG CACCCTGTGG CGAGCACTGG CCTTGGAGCC CGGCCTCGCT 
GCACAGGTCC TGGGGCTGCT CCTGGAGAAG ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC 
CAGGCCTTCC TGCTGAGCAG CACGCCGGAC CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC 
ACCTGTGCAC TGCACGAGGT CGCATCTGCC CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC 
TACCCCCAGC TGTTTGTGGC ACTGCTGCTG CGGGTCAGCT GCACCGTGGG TGTCCAGCCG 
CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG AGCGCCAGCT CGGGCCTGGC CTCACGGAGC 
CTCGAGCCTT GCAGCTCTGC GGTGGACGCG CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT 
GAGGATGTGG TCCAGTGCAT GGAGCTGGAC GGGGGCTGGC AGTTGCTCAG GACCTCGGCT 
GGGCACGAGG AAGGTGTCAC CCGGCTGGCC AGTGCCATGG CAAAGTTCGC AGGCCCCCGG 
CTGCCCCTGG TGATGAAGCT GCTCTTCACC ACACAGAGTA GCATGTATGA GGTCCAGAGG 
GTCACCTCCA CAGCCTTCCT GGCTGAGCCA TGTCCCCTCA GTGACCAGGG GCCGGTGCTG 
CCCGGTGCAC TTGGGCGGGT GTGGTGGTCA GCACGCCTCT GCCCCCAGCT GCTCAGCAGC 
AATGTGGTGA ACGACCTGAT GCTCCTGGAG TCACTGCTGT ACAACCTGAT GGCACGGCAG 
AAGGACACGA GCGCCCGCGT GCGGAGGCTG GTGCTCCACG GCCTGGCCAA CATCACCTTG 
GGCTCCCCAG ATAAGGTACA GACCCACAGC CCCCAACTCC TGACAGCCAT GATCGGTGGG 
CTGGACGACG GGGACGACCC ACACAGCCTG GTGGCGCTGG AGGCCATGGT GGGCCTGGCA 
AGGCTGATGG ACCTGGTGGA CGCCTGGGAC CTACATGCAG TGCTGCTGCA CATTGCTGTC 
CGCATTCGGC CCTTCTTCGA CAGCGAAAGG ATGGAGTTAC GCTCAGTGTC CATTGGCCTC 
TTCGGGCACC TCAACAAGGC CTGCCGTGGG GACTGCAAGG ACGTGTTCCT GGAGCAGGTT 
GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC CTGCGTGACC CCCACGCACC CGTGGTCACG 
GCCTGCAGGT TCGCCTTGCG CATGTGCGGC CCCAACCTGG AGTGTGAGGA GCTGGCAGCT 
GTCTTCCAGA GGCACCTGCA GGAAGGCCAT GACCTGCACT TTGGAGAGTT CCTCAACACC 
ACCTGCAAGC ACCTGATGCG CCACTTCCCC GACCTGCTGG GCCGCTTGCT GAGCACCAGT 
CTGTTCTACT ATAAGAGCAG CTGGGAGGAT GTCCGTGCTG CTGCCCCCAT GCTCACAGGG 
TTCTTGGTGC TGCACATGGA GGCTGAGCAG CGGCCACAGG TGGACCTGGA GCAGCTCCTC 
ACGGCACTCC AGCTGCTGCT CAAGGACCCA GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG 
ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001540.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857308.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.

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CloneID	OBa222279
Clone ID Related Accession (Same CDS sequence)	XM_025001540.1
Accession Version	XM_025001540.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857308.1
CDS	161..5218
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001540.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001540.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001541.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857309.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.

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CloneID	OBa222280
Clone ID Related Accession (Same CDS sequence)	XM_025001541.1
Accession Version	XM_025001541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5031bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GACCCGGCCC TGAGACTGAG CCTGGTGCAA AGTGTGTGCA TGGCCACCCA GGCCATCTGC 
AGCAGTGCCC ACAGCAGCTC CTTCCACCTC TCGAGGAAGG CGGAGCTGGT GGCGCAGATG 
GTGGAGTTCA TCAGAGCGGA GCCCCCAGAC TCGCTGAAGA CGCCCATTTG GAAGAAGGCC 
ATGCTTGCCT GCACGTACCT GGTTACCCTG GAGCCGGCCC TGGAGGAGCA GGTGCAGGCG 
GACCTGATTC ACAGCTGCCT GCACCGTGTC ATGGCCGTGC TGCCGGAGCC AGAGGAGGGG 
GACAGCCCCC AGGAGTCCCT GTACCTGGAC ACCGTGCAGG TCCTCAAGGA CTTGCTGACG 
AGCCTTCTTC TGTGGAACAT GACGCCCCTG GGTCTGCAGG TCATGTTGGA GCACCTGAGC 
CCATGGATCA AGTCCCCGAG GGGCCACGAG CGGGTGCGGG CGGTCGGCCT GAGTGCCTGC 
CTGCTGCAGT TCTTCCTTCA ACACCTGCAG ATCAGCGCCC TGGTGCCCTT CCACAACCTG 
GGCCTCCTCA TTGGCCTCTT CTCCCCACGG TGCGCCGACC TCTGGCCTGC CACTCGCCGA 
GAGGCTGTGA GCTGCATCCA CTCCCTGCTA TACCTGCAGC TGACCTGTGT GGGCTTCTCG 
CGAGACTACC AGGACGACGT GGCTGAGCAG CTCCTCAGCC TCAAAGATGG CCTGGTGCAC 
CCTGACCCTG CCATTCTCTT CCATACCTGC CACAGCATTG CCCAGCTTAT TGCCAAGCGC 
CTCCCTCCAG ATCAGCTCAT CAACCTCTTG CTAACCTTGT TTGAGAGCCT GGGAGATCCC 
GACAAGAACT GCTCGAGAGC TGCCAGCGTC ATGATCAACT GCCTGCTGAA GGAACGGGGC 
AACATGCTGC AGGAGAAGGT GCCCGAGATT GTGAGTGTGC TGCGCTCCAA GCTGCAGGAG 
ACCCAAGAGG AGCACATCCT ACAGGCCGCA CAGCACAGCG TGTTTGCCCT GGCCACCCAC 
CACTGTGCCT CTGTGGTGTC CAACCTTCTG GGCAGCCCCC TGCCCTTTGA CAGCCACACC 
TGCACCCTGT GGCGAGCACT GGCCTTGGAG CCCGGCCTCG CTGCACAGGT CCTGGGGCTG 
CTCCTGGAGA AGATAAGCAA GGACGTCCTG TTTGAGGAGA GCCAGGCCTT CCTGCTGAGC 
AGCACGCCGG ACCGCGTGGC CACCCTGCTG CCCCTCGCAG CCACCTGTGC ACTGCACGAG 
GTCGCATCTG CCCCGGCGTC CGGGCCAGCA GTGCTGGAGC TCTACCCCCA GCTGTTTGTG 
GCACTGCTGC TGCGGGTCAG CTGCACCGTG GGTGTCCAGC CGCCCAGGCA GCTGCAGGCC 
AAGGAGAGGA GGAGCGCCAG CTCGGGCCTG GCCTCACGGA GCCTCGAGCC TTGCAGCTCT 
GCGGTGGACG CGCTGCAGGC CGTGCTTGTC CGCGGTGGCA ATGAGGATGT GGTCCAGTGC 
ATGGAGCTGG ACGGGGGCTG GCAGTTGCTC AGGACCTCGG CTGGGCACGA GGAAGGTGTC 
ACCCGGCTGG CCAGTGCCAT GGCAAAGTTC GCAGGCCCCC GGCTGCCCCT GGTGATGAAG 
CTGCTCTTCA CCACACAGAG TAGCATGTAT GAGGTCCAGA GGGTCACCTC CACAGCCTTC 
CTGGCTGAGC TGCTCAGCAG CAATGTGGTG AACGACCTGA TGCTCCTGGA GTCACTGCTG 
TACAACCTGA TGGCACGGCA GAAGGACACG AGCGCCCGCG TGCGGAGGCT GGTGCTCCAC 
GGCCTGGCCA ACATCACCTT GGGCTCCCCA GATAAGGTAC AGACCCACAG CCCCCAACTC 
CTGACAGCCA TGATCGGTGG GCTGGACGAC GGGGACGACC CACACAGCCT GGTGGCGCTG 
GAGGCCATGG TGGGCCTGGC AAGGCTGATG GACCTGGTGG ACGCCTGGGA CCTACATGCA 
GTGCTGCTGC ACATTGCTGT CCGCATTCGG CCCTTCTTCG ACAGCGAAAG GATGGAGTTA 
CGCTCAGTGT CCATTGGCCT CTTCGGGCAC CTCAACAAGG CCTGCCGTGG GGACTGCAAG 
GACGTGTTCC TGGAGCAGGT TGTGGGCGGC CTGGTGCCCC TTCTGCTGCA CCTGCGTGAC 
CCCCACGCAC CCGTGGTCAC GGCCTGCAGG TTCGCCTTGC GCATGTGCGG CCCCAACCTG 
GAGTGTGAGG AGCTGGCAGC TGTCTTCCAG AGGCACCTGC AGGAAGGCCA TGACCTGCAC 
TTTGGAGAGT TCCTCAACAC CACCTGCAAG CACCTGATGC GCCACTTCCC CGACCTGCTG 
GGCCGCTTGC TGAGCACCAG TCTGTTCTAC TATAAGAGCA GCTGGGAGGA TGTCCGTGCT 
GCTGCCCCCA TGCTCACAGG GTTCTTGGTG CTGCACATGG AGGCTGAGCA GCGGCCACAG 
GTGGACCTGG AGCAGCTCCT CACGGCACTC CAGCTGCTGC TCAAGGACCC AGCCCTTAAG 
GTGCGCTTGA AGGCTGTGAA GACTCTGGGC CGCTTGGTGA AGTTCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857309.1
CDS	161..5191
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001541.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001541.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGGTGAG AGAAGAGGAG 
AAGACGAGAG TTAGCCTGCT TTGGGAACAG GAGGAGAAGA CGAGATTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GACCCGGCCC TGAGACTGAG CCTGGTGCAA AGTGTGTGCA TGGCCACCCA GGCCATCTGC 
AGCAGTGCCC ACAGCAGCTC CTTCCACCTC TCGAGGAAGG CGGAGCTGGT GGCGCAGATG 
GTGGAGTTCA TCAGAGCGGA GCCCCCAGAC TCGCTGAAGA CGCCCATTTG GAAGAAGGCC 
ATGCTTGCCT GCACGTACCT GGTTACCCTG GAGCCGGCCC TGGAGGAGCA GGTGCAGGCG 
GACCTGATTC ACAGCTGCCT GCACCGTGTC ATGGCCGTGC TGCCGGAGCC AGAGGAGGGG 
GACAGCCCCC AGGAGTCCCT GTACCTGGAC ACCGTGCAGG TCCTCAAGGA CTTGCTGACG 
AGCCTTCTTC TGTGGAACAT GACGCCCCTG GGTCTGCAGG TCATGTTGGA GCACCTGAGC 
CCATGGATCA AGTCCCCGAG GGGCCACGAG CGGGTGCGGG CGGTCGGCCT GAGTGCCTGC 
CTGCTGCAGT TCTTCCTTCA ACACCTGCAG ATCAGCGCCC TGGTGCCCTT CCACAACCTG 
GGCCTCCTCA TTGGCCTCTT CTCCCCACGG TGCGCCGACC TCTGGCCTGC CACTCGCCGA 
GAGGCTGTGA GCTGCATCCA CTCCCTGCTA TACCTGCAGC TGACCTGTGT GGGCTTCTCG 
CGAGACTACC AGGACGACGT GGCTGAGCAG CTCCTCAGCC TCAAAGATGG CCTGGTGCAC 
CCTGACCCTG CCATTCTCTT CCATACCTGC CACAGCATTG CCCAGCTTAT TGCCAAGCGC 
CTCCCTCCAG ATCAGCTCAT CAACCTCTTG CTAACCTTGT TTGAGAGCCT GGGAGATCCC 
GACAAGAACT GCTCGAGAGC TGCCAGCGTC ATGATCAACT GCCTGCTGAA GGAACGGGGC 
AACATGCTGC AGGAGAAGGT GCCCGAGATT GTGAGTGTGC TGCGCTCCAA GCTGCAGGAG 
ACCCAAGAGG AGCACATCCT ACAGGCCGCA CAGCACAGCG TGTTTGCCCT GGCCACCCAC 
CACTGTGCCT CTGTGGTGTC CAACCTTCTG GGCAGCCCCC TGCCCTTTGA CAGCCACACC 
TGCACCCTGT GGCGAGCACT GGCCTTGGAG CCCGGCCTCG CTGCACAGGT CCTGGGGCTG 
CTCCTGGAGA AGATAAGCAA GGACGTCCTG TTTGAGGAGA GCCAGGCCTT CCTGCTGAGC 
AGCACGCCGG ACCGCGTGGC CACCCTGCTG CCCCTCGCAG CCACCTGTGC ACTGCACGAG 
GTCGCATCTG CCCCGGCGTC CGGGCCAGCA GTGCTGGAGC TCTACCCCCA GCTGTTTGTG 
GCACTGCTGC TGCGGGTCAG CTGCACCGTG GGTGTCCAGC CGCCCAGGCA GCTGCAGGCC 
AAGGAGAGGA GGAGCGCCAG CTCGGGCCTG GCCTCACGGA GCCTCGAGCC TTGCAGCTCT 
GCGGTGGACG CGCTGCAGGC CGTGCTTGTC CGCGGTGGCA ATGAGGATGT GGTCCAGTGC 
ATGGAGCTGG ACGGGGGCTG GCAGTTGCTC AGGACCTCGG CTGGGCACGA GGAAGGTGTC 
ACCCGGCTGG CCAGTGCCAT GGCAAAGTTC GCAGGCCCCC GGCTGCCCCT GGTGATGAAG 
CTGCTCTTCA CCACACAGAG TAGCATGTAT GAGGTCCAGA GGGTCACCTC CACAGCCTTC 
CTGGCTGAGC TGCTCAGCAG CAATGTGGTG AACGACCTGA TGCTCCTGGA GTCACTGCTG 
TACAACCTGA TGGCACGGCA GAAGGACACG AGCGCCCGCG TGCGGAGGCT GGTGCTCCAC 
GGCCTGGCCA ACATCACCTT GGGCTCCCCA GATAAGGTAC AGACCCACAG CCCCCAACTC 
CTGACAGCCA TGATCGGTGG GCTGGACGAC GGGGACGACC CACACAGCCT GGTGGCGCTG 
GAGGCCATGG TGGGCCTGGC AAGGCTGATG GACCTGGTGG ACGCCTGGGA CCTACATGCA 
GTGCTGCTGC ACATTGCTGT CCGCATTCGG CCCTTCTTCG ACAGCGAAAG GATGGAGTTA 
CGCTCAGTGT CCATTGGCCT CTTCGGGCAC CTCAACAAGG CCTGCCGTGG GGACTGCAAG 
GACGTGTTCC TGGAGCAGGT TGTGGGCGGC CTGGTGCCCC TTCTGCTGCA CCTGCGTGAC 
CCCCACGCAC CCGTGGTCAC GGCCTGCAGG TTCGCCTTGC GCATGTGCGG CCCCAACCTG 
GAGTGTGAGG AGCTGGCAGC TGTCTTCCAG AGGCACCTGC AGGAAGGCCA TGACCTGCAC 
TTTGGAGAGT TCCTCAACAC CACCTGCAAG CACCTGATGC GCCACTTCCC CGACCTGCTG 
GGCCGCTTGC TGAGCACCAG TCTGTTCTAC TATAAGAGCA GCTGGGAGGA TGTCCGTGCT 
GCTGCCCCCA TGCTCACAGG GTTCTTGGTG CTGCACATGG AGGCTGAGCA GCGGCCACAG 
GTGGACCTGG AGCAGCTCCT CACGGCACTC CAGCTGCTGC TCAAGGACCC AGCCCTTAAG 
GTGCGCTTGA AGGCTGTGAA GACTCTGGGC CGCTTGGTGA AGTTCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001542.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857310.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.

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CloneID	OBa222281
Clone ID Related Accession (Same CDS sequence)	XM_025001542.1
Accession Version	XM_025001542.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5019bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGTCCCTGT ACCTGGACAC CGTGCAGGTC CTCAAGGACT TGCTGACGAG CCTTCTTCTG 
TGGAACATGA CGCCCCTGGG TCTGCAGGTC ATGTTGGAGC ACCTGAGCCC ATGGATCAAG 
TCCCCGAGGG GCCACGAGCG GGTGCGGGCG GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC 
TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG GTGCCCTTCC ACAACCTGGG CCTCCTCATT 
GGCCTCTTCT CCCCACGGTG CGCCGACCTC TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC 
TGCATCCACT CCCTGCTATA CCTGCAGCTG ACCTGTGTGG GCTTCTCGCG AGACTACCAG 
GACGACGTGG CTGAGCAGCT CCTCAGCCTC AAAGATGGCC TGGTGCACCC TGACCCTGCC 
ATTCTCTTCC ATACCTGCCA CAGCATTGCC CAGCTTATTG CCAAGCGCCT CCCTCCAGAT 
CAGCTCATCA ACCTCTTGCT AACCTTGTTT GAGAGCCTGG GAGATCCCGA CAAGAACTGC 
TCGAGAGCTG CCAGCGTCAT GATCAACTGC CTGCTGAAGG AACGGGGCAA CATGCTGCAG 
GAGAAGGTGC CCGAGATTGT GAGTGTGCTG CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG 
CACATCCTAC AGGCCGCACA GCACAGCGTG TTTGCCCTGG CCACCCACCA CTGTGCCTCT 
GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG CCCTTTGACA GCCACACCTG CACCCTGTGG 
CGAGCACTGG CCTTGGAGCC CGGCCTCGCT GCACAGGTCC TGGGGCTGCT CCTGGAGAAG 
ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC CAGGCCTTCC TGCTGAGCAG CACGCCGGAC 
CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC ACCTGTGCAC TGCACGAGGT CGCATCTGCC 
CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC TACCCCCAGC TGTTTGTGGC ACTGCTGCTG 
CGGGTCAGCT GCACCGTGGG TGTCCAGCCG CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG 
AGCGCCAGCT CGGGCCTGGC CTCACGGAGC CTCGAGCCTT GCAGCTCTGC GGTGGACGCG 
CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT GAGGATGTGG TCCAGTGCAT GGAGCTGGAC 
GGGGGCTGGC AGTTGCTCAG GACCTCGGCT GGGCACGAGG AAGGTGTCAC CCGGCTGGCC 
AGTGCCATGG CAAAGTTCGC AGGCCCCCGG CTGCCCCTGG TGATGAAGCT GCTCTTCACC 
ACACAGAGTA GCATGTATGA GGTCCAGAGG GTCACCTCCA CAGCCTTCCT GGCTGAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857310.1
CDS	161..5179
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001542.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001542.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGTCCCTGT ACCTGGACAC CGTGCAGGTC CTCAAGGACT TGCTGACGAG CCTTCTTCTG 
TGGAACATGA CGCCCCTGGG TCTGCAGGTC ATGTTGGAGC ACCTGAGCCC ATGGATCAAG 
TCCCCGAGGG GCCACGAGCG GGTGCGGGCG GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC 
TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG GTGCCCTTCC ACAACCTGGG CCTCCTCATT 
GGCCTCTTCT CCCCACGGTG CGCCGACCTC TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC 
TGCATCCACT CCCTGCTATA CCTGCAGCTG ACCTGTGTGG GCTTCTCGCG AGACTACCAG 
GACGACGTGG CTGAGCAGCT CCTCAGCCTC AAAGATGGCC TGGTGCACCC TGACCCTGCC 
ATTCTCTTCC ATACCTGCCA CAGCATTGCC CAGCTTATTG CCAAGCGCCT CCCTCCAGAT 
CAGCTCATCA ACCTCTTGCT AACCTTGTTT GAGAGCCTGG GAGATCCCGA CAAGAACTGC 
TCGAGAGCTG CCAGCGTCAT GATCAACTGC CTGCTGAAGG AACGGGGCAA CATGCTGCAG 
GAGAAGGTGC CCGAGATTGT GAGTGTGCTG CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG 
CACATCCTAC AGGCCGCACA GCACAGCGTG TTTGCCCTGG CCACCCACCA CTGTGCCTCT 
GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG CCCTTTGACA GCCACACCTG CACCCTGTGG 
CGAGCACTGG CCTTGGAGCC CGGCCTCGCT GCACAGGTCC TGGGGCTGCT CCTGGAGAAG 
ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC CAGGCCTTCC TGCTGAGCAG CACGCCGGAC 
CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC ACCTGTGCAC TGCACGAGGT CGCATCTGCC 
CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC TACCCCCAGC TGTTTGTGGC ACTGCTGCTG 
CGGGTCAGCT GCACCGTGGG TGTCCAGCCG CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG 
AGCGCCAGCT CGGGCCTGGC CTCACGGAGC CTCGAGCCTT GCAGCTCTGC GGTGGACGCG 
CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT GAGGATGTGG TCCAGTGCAT GGAGCTGGAC 
GGGGGCTGGC AGTTGCTCAG GACCTCGGCT GGGCACGAGG AAGGTGTCAC CCGGCTGGCC 
AGTGCCATGG CAAAGTTCGC AGGCCCCCGG CTGCCCCTGG TGATGAAGCT GCTCTTCACC 
ACACAGAGTA GCATGTATGA GGTCCAGAGG GTCACCTCCA CAGCCTTCCT GGCTGAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001543.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857311.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBa222282
Clone ID Related Accession (Same CDS sequence)	XM_025001543.1
Accession Version	XM_025001543.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGTTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857311.1
CDS	161..5158
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEAVSSGPEPTTGDLQSRVHSILPSRPQIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001543.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001543.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGGC CGTGTCCTCA GGGCCAGAGC CCACGACTGG TGACCTGCAG 
AGCAGAGTGC ACAGCATCCT GCCCTCCCGC CCCCAGATTC AGAAGCTGGG TGCCGACAGT 
GAGGCCCTGG CGGCCGATAG CGTGAGGGCC ATCAGCGTCA GCACTCTCTA CCTGGTCAGC 
ACCACTGTGG ACAGGATGGG TGAGGTCCTC TGGCCGTACC TGCTTGAGTT CCTGGTCCCC 
ATTCGCTTCA CCAGGGCGCT GAGCCCACTC TGCAGGAGCC TTGTGCACTT GGCCCAGAAG 
AGGCAGGAGG CGGGGGCCCA TGCTCCCCTC ATCCAGTACA ACGGCAACGT GCACCTCCCG 
TCTCCCTACG CCATCACCAC CAGACTCCTG GCCGTGTCTT CCCATCCCTA TGTGGGGGAC 
GGTCGCGGGG CAGCCTCGCT GCGCCTCTTG AATGTCTTGC ATCAGGACAT CCACCCGGCC 
CTGGGTCAGC GGTGGGTGAC CGCCATCCCC CTGCTGCTGG AGCACCTGGA CGAATACAGT 
GAAGAAACGC TGTCACAGAA GGAGTGGGAA GAAAAGCTGC TGGTGTTTCT CCGGGATTCC 
CTGGCTGTCG TGTCTGACAA CACCTGGGTC TGCCACCTGA CCCTGGAAAT GTGCAAGCAG 
CTACCCAACT ACAACGGGAC GCCCCTGGAG AAGAACTTCC TGTACAAATG TGTCGGGACC 
ACCCTGGGTG CCGCTTCAAG TAAGGTGGTG AGGAAGCACC TGCGGGAGCT GCTGGAGACG 
GCCCGATACC AGGAGGAGAG GGAGCACGAG GGCCTTGCCT GTTGCTTTGG GATCTGTGCC 
ATCTCCCACC TGGATGACAC CTTGGCCCAG CTGGACGACT TTGTGAAGTC AGACGTACTC 
AGAAAATCTG CTGGCATTTT CAACCTTTTT AAGAATCGAA GTGAGAGTGA GGCCAACAAA 
GTGAGGAGTG CGCTGATCCT GTGCTATGGG CATGTGGCGG CCGGGGCCCC GCGCGAGCTG 
CTGCTGGCCA GGGTGGAGGC GGACTTGTTC TGGAACATGT CCGAGTGCTT CAGCACCAAG 
GTTCTGGGGA TAAAGGTAGA AACCCAGGAC CCGGCCCTGA GACTGAGCCT GGTGCAAAGT 
GTGTGCATGG CCACCCAGGC CATCTGCAGC AGTGCCCACA GCAGCTCCTT CCACCTCTCG 
AGGAAGGCGG AGCTGGTGGC GCAGATGGTG GAGTTCATCA GAGCGGAGCC CCCAGACTCG 
CTGAAGACGC CCATTTGGAA GAAGGCCATG CTTGCCTGCA CGTACCTGGT TACCCTGGAG 
CCGGCCCTGG AGGAGCAGGT GCAGGCGGAC CTGATTCACA GCTGCCTGCA CCGTGTCATG 
GCCGTGCTGC CGGAGCCAGA GGAGGGGGAC AGCCCCCAGG AGTCCCTGTA CCTGGACACC 
GTGCAGGTCC TCAAGGACTT GCTGACGAGC CTTCTTCTGT GGAACATGAC GCCCCTGGGT 
CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA TGGATCAAGT CCCCGAGGGG CCACGAGCGG 
GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG CTGCAGTTCT TCCTTCAACA CCTGCAGATC 
AGCGCCCTGG TGCCCTTCCA CAACCTGGGC CTCCTCATTG GCCTCTTCTC CCCACGGTGC 
GCCGACCTCT GGCCTGCCAC TCGCCGAGAG GCTGTGAGCT GCATCCACTC CCTGCTATAC 
CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA GACTACCAGG ACGACGTGGC TGAGCAGCTC 
CTCAGCCTCA AAGATGGCCT GGTGCACCCT GACCCTGCCA TTCTCTTCCA TACCTGCCAC 
AGCATTGCCC AGCTTATTGC CAAGCGCCTC CCTCCAGATC AGCTCATCAA CCTCTTGCTA 
ACCTTGTTTG AGAGCCTGGG AGATCCCGAC AAGAACTGCT CGAGAGCTGC CAGCGTCATG 
ATCAACTGCC TGCTGAAGGA ACGGGGCAAC ATGCTGCAGG AGAAGGTGCC CGAGATTGTG 
AGTGTGCTGC GCTCCAAGCT GCAGGAGACC CAAGAGGAGC ACATCCTACA GGCCGCACAG 
CACAGCGTGT TTGCCCTGGC CACCCACCAC TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC 
AGCCCCCTGC CCTTTGACAG CCACACCTGC ACCCTGTGGC GAGCACTGGC CTTGGAGCCC 
GGCCTCGCTG CACAGGTCCT GGGGCTGCTC CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT 
GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC ACGCCGGACC GCGTGGCCAC CCTGCTGCCC 
CTCGCAGCCA CCTGTGCACT GCACGAGGTC GCATCTGCCC CGGCGTCCGG GCCAGCAGTG 
CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA CTGCTGCTGC GGGTCAGCTG CACCGTGGGT 
GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC 
TCACGGAGCC TCGAGCCTTG CAGCTCTGCG GTGGACGCGC TGCAGGCCGT GCTTGTCCGC 
GGTGGCAATG AGGATGTGGT CCAGTGCATG GAGCTGGACG GGGGCTGGCA GTTGCTCAGG 
ACCTCGGCTG GGCACGAGGA AGGTGTCACC CGGCTGGCCA GTGCCATGGC AAAGTTCGCA 
GGCCCCCGGC TGCCCCTGGT GATGAAGCTG CTCTTCACCA CACAGAGTAG CATGTATGAG 
GTCCAGAGGG TCACCTCCAC AGCCTTCCTG GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC 
GACCTGATGC TCCTGGAGTC ACTGCTGTAC AACCTGATGG CACGGCAGAA GGACACGAGC 
GCCCGCGTGC GGAGGCTGGT GCTCCACGGC CTGGCCAACA TCACCTTGGG CTCCCCAGAT 
AAGGTACAGA CCCACAGCCC CCAACTCCTG ACAGCCATGA TCGGTGGGCT GGACGACGGG 
GACGACCCAC ACAGCCTGGT GGCGCTGGAG GCCATGGTGG GCCTGGCAAG GCTGATGGAC 
CTGGTGGACG CCTGGGACCT ACATGCAGTG CTGCTGCACA TTGCTGTCCG CATTCGGCCC 
TTCTTCGACA GCGAAAGGAT GGAGTTACGC TCAGTGTCCA TTGGCCTCTT CGGGCACCTC 
AACAAGGCCT GCCGTGGGGA CTGCAAGGAC GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG 
GTGCCCCTTC TGCTGCACCT GCGTGACCCC CACGCACCCG TGGTCACGGC CTGCAGGTTC 
GCCTTGCGCA TGTGCGGCCC CAACCTGGAG TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG 
CACCTGCAGG AAGGCCATGA CCTGCACTTT GGAGAGTTCC TCAACACCAC CTGCAAGCAC 
CTGATGCGCC ACTTCCCCGA CCTGCTGGGC CGCTTGCTGA GCACCAGTCT GTTCTACTAT 
AAGAGCAGCT GGGAGGATGT CCGTGCTGCT GCCCCCATGC TCACAGGGTT CTTGGTGCTG 
CACATGGAGG CTGAGCAGCG GCCACAGGTG GACCTGGAGC AGCTCCTCAC GGCACTCCAG 
CTGCTGCTCA AGGACCCAGC CCTTAAGGTG CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC 
TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_025001544.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024857312.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBa222283
Clone ID Related Accession (Same CDS sequence)	XM_025001544.1
Accession Version	XM_025001544.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4974bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCCATGTCCC 
CTCAGTGACC AGGGGCCGGT GCTGCCCGGT GCACTTGGGC GGGTGTGGTG GTCAGCACGC 
CTCTGCCCCC AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024857312.1
CDS	161..5134
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAEPCPLSDQGPVLPGALGRVWWSARLCPQLLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_025001544.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025001544.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCCATGTCCC 
CTCAGTGACC AGGGGCCGGT GCTGCCCGGT GCACTTGGGC GGGTGTGGTG GTCAGCACGC 
CTCTGCCCCC AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( NM_001102018.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095488.1 Bos taurus maestro heat like repeat family member 1 (MROH1), mRNA.

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CloneID	OBa43997
Clone ID Related Accession (Same CDS sequence)	NM_001102018.1
Accession Version	NM_001102018.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4959bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151617.1. On Aug 21, 2007 this sequence version replaced XM_593002.3. ##Evidence-Data-START## Transcript exon combination :: BC151617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGTCCCTGT ACCTGGACAC CGTGCAGGTC CTCAAGGACT TGCTGACGAG CCTTCTTCTG 
TGGAACATGA CGCCCCTGGG TCTGCAGGTC ATGTTGGAGC ACCTGAGCCC ATGGATCAAG 
TCCCCGAGGG GCCACGAGCG GGTGCGGGCG GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC 
TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG GTGCCCTTCC ACAACCTGGG CCTCCTCATT 
GGCCTCTTCT CCCCACGGTG CGCCGACCTC TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC 
TGCATCCACT CCCTGCTATA CCTGCAGCTG ACCTGTGTGG GCTTCTCGCG AGACTACCAG 
GACGACGTGG CTGAGCAGCT CCTCAGCCTC AAAGATGGCC TGGTGCACCC TGACCCTGCC 
ATTCTCTTCC ATACCTGCCA CAGCATTGCC CAGCTTATTG CCAAGCGCCT CCCTCCAGAT 
CATCTCATCA ACCTCTTGCT AACCTTGTTT GAGAGCCTGG GAGATCCCGA CAAGAACTGC 
TCGAGAGCTG CCAGCGTCAT GATCAACTGC CTGCTGAAGG AACGGGGCAA CATGCTGCAG 
GAGAAGGTGC CCGAGATTGT GAGTGTGCTG CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG 
CACATCCTAC AGGCCGCACA GCACAGCGTG TTTGCCCTGG CCACCCACCA CTGTGCCTCT 
GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG CCCTTTGACA GCCACACCTG CACCCTGTGG 
CGAGCACTGG CCTTGGAGCC CGGCCTCGCT GCACAGGTCC TGGGGCTGCT CCTGGAGAAG 
ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC CAGGCCTTCC TGCTGAGCAG CACGCCGGAC 
CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC ACCTGTGCAC TGCACGAGGT CGCATCTGCC 
CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC TACCCCCAGC TGTTTGTGGC ACTGCTGCTG 
CGGGTCAGCT GCACCGTGGG TGTCCAGCCG CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG 
AGCGCCAGCT CGGGCCTGGC CTCACGGAGC CTCGAGCCTT GCAGCTCTGC GGTGGACGCG 
CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT GAGGATGTGG TCCAGTGCAT GGAGCTGGAC 
GGGGGCTGGC AGTTGCTCAG GACCTCGGCT GGGCACGAGG AAGGTGTCAC CCGGCTGGCC 
AGTGCCATGG CAAAGTTCGC AGGCCCCCGG CTGCCCCTGG TGATGAAGCT GCTCTTCACC 
ACACAGAGTA GCATGTATGA GGTCCAGAGG GTCACCTCCA CAGCCTTCCT GGCTGAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095488.1
CDS	116..5074
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDHLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	NM_001102018.1
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001102018.1

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGTCCCTGT ACCTGGACAC CGTGCAGGTC CTCAAGGACT TGCTGACGAG CCTTCTTCTG 
TGGAACATGA CGCCCCTGGG TCTGCAGGTC ATGTTGGAGC ACCTGAGCCC ATGGATCAAG 
TCCCCGAGGG GCCACGAGCG GGTGCGGGCG GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC 
TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG GTGCCCTTCC ACAACCTGGG CCTCCTCATT 
GGCCTCTTCT CCCCACGGTG CGCCGACCTC TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC 
TGCATCCACT CCCTGCTATA CCTGCAGCTG ACCTGTGTGG GCTTCTCGCG AGACTACCAG 
GACGACGTGG CTGAGCAGCT CCTCAGCCTC AAAGATGGCC TGGTGCACCC TGACCCTGCC 
ATTCTCTTCC ATACCTGCCA CAGCATTGCC CAGCTTATTG CCAAGCGCCT CCCTCCAGAT 
CATCTCATCA ACCTCTTGCT AACCTTGTTT GAGAGCCTGG GAGATCCCGA CAAGAACTGC 
TCGAGAGCTG CCAGCGTCAT GATCAACTGC CTGCTGAAGG AACGGGGCAA CATGCTGCAG 
GAGAAGGTGC CCGAGATTGT GAGTGTGCTG CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG 
CACATCCTAC AGGCCGCACA GCACAGCGTG TTTGCCCTGG CCACCCACCA CTGTGCCTCT 
GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG CCCTTTGACA GCCACACCTG CACCCTGTGG 
CGAGCACTGG CCTTGGAGCC CGGCCTCGCT GCACAGGTCC TGGGGCTGCT CCTGGAGAAG 
ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC CAGGCCTTCC TGCTGAGCAG CACGCCGGAC 
CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC ACCTGTGCAC TGCACGAGGT CGCATCTGCC 
CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC TACCCCCAGC TGTTTGTGGC ACTGCTGCTG 
CGGGTCAGCT GCACCGTGGG TGTCCAGCCG CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG 
AGCGCCAGCT CGGGCCTGGC CTCACGGAGC CTCGAGCCTT GCAGCTCTGC GGTGGACGCG 
CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT GAGGATGTGG TCCAGTGCAT GGAGCTGGAC 
GGGGGCTGGC AGTTGCTCAG GACCTCGGCT GGGCACGAGG AAGGTGTCAC CCGGCTGGCC 
AGTGCCATGG CAAAGTTCGC AGGCCCCCGG CTGCCCCTGG TGATGAAGCT GCTCTTCACC 
ACACAGAGTA GCATGTATGA GGTCCAGAGG GTCACCTCCA CAGCCTTCCT GGCTGAGCTG 
CTCAGCAGCA ATGTGGTGAA CGACCTGATG CTCCTGGAGT CACTGCTGTA CAACCTGATG 
GCACGGCAGA AGGACACGAG CGCCCGCGTG CGGAGGCTGG TGCTCCACGG CCTGGCCAAC 
ATCACCTTGG GCTCCCCAGA TAAGGTACAG ACCCACAGCC CCCAACTCCT GACAGCCATG 
ATCGGTGGGC TGGACGACGG GGACGACCCA CACAGCCTGG TGGCGCTGGA GGCCATGGTG 
GGCCTGGCAA GGCTGATGGA CCTGGTGGAC GCCTGGGACC TACATGCAGT GCTGCTGCAC 
ATTGCTGTCC GCATTCGGCC CTTCTTCGAC AGCGAAAGGA TGGAGTTACG CTCAGTGTCC 
ATTGGCCTCT TCGGGCACCT CAACAAGGCC TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG 
GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT CTGCTGCACC TGCGTGACCC CCACGCACCC 
GTGGTCACGG CCTGCAGGTT CGCCTTGCGC ATGTGCGGCC CCAACCTGGA GTGTGAGGAG 
CTGGCAGCTG TCTTCCAGAG GCACCTGCAG GAAGGCCATG ACCTGCACTT TGGAGAGTTC 
CTCAACACCA CCTGCAAGCA CCTGATGCGC CACTTCCCCG ACCTGCTGGG CCGCTTGCTG 
AGCACCAGTC TGTTCTACTA TAAGAGCAGC TGGGAGGATG TCCGTGCTGC TGCCCCCATG 
CTCACAGGGT TCTTGGTGCT GCACATGGAG GCTGAGCAGC GGCCACAGGT GGACCTGGAG 
CAGCTCCTCA CGGCACTCCA GCTGCTGCTC AAGGACCCAG CCCTTAAGGT GCGCTTGAAG 
GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811840.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810142.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.

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CloneID	OBa49177
Clone ID Related Accession (Same CDS sequence)	XM_010811840.2 , XM_010811837.2 , XM_010811839.2 , XM_010811838.2
Accession Version	XM_010811840.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811840.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810142.1
CDS	163..5250
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811840.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811840.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811837.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810139.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.

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CloneID	OBa49177
Clone ID Related Accession (Same CDS sequence)	XM_010811840.2 , XM_010811837.2 , XM_010811839.2 , XM_010811838.2
Accession Version	XM_010811837.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810139.1
CDS	163..5250
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811837.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811837.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811839.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810141.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.

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CloneID	OBa49177
Clone ID Related Accession (Same CDS sequence)	XM_010811840.2 , XM_010811837.2 , XM_010811839.2 , XM_010811838.2
Accession Version	XM_010811839.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810141.1
CDS	316..5403
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811839.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811839.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811838.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810140.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.

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CloneID	OBa49177
Clone ID Related Accession (Same CDS sequence)	XM_010811840.2 , XM_010811837.2 , XM_010811839.2 , XM_010811838.2
Accession Version	XM_010811838.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811838.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810140.1
CDS	380..5467
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811838.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811838.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811841.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810143.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.

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CloneID	OBa49178
Clone ID Related Accession (Same CDS sequence)	XM_010811841.2
Accession Version	XM_010811841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811841.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810143.1
CDS	163..5235
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811841.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811841.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811842.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810144.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa49179
Clone ID Related Accession (Same CDS sequence)	XM_010811842.2
Accession Version	XM_010811842.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811842.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGACC CGGCCCTGAG ACTGAGCCTG 
GTGCAAAGTG TGTGCATGGC CACCCAGGCC ATCTGCAGCA GTGCCCACAG CAGCTCCTTC 
CACCTCTCGA GGAAGGCGGA GCTGGTGGCG CAGATGGTGG AGTTCATCAG AGCGGAGCCC 
CCAGACTCGC TGAAGACGCC CATTTGGAAG AAGGCCATGC TTGCCTGCAC GTACCTGGTT 
ACCCTGGAGC CGGCCCTGGA GGAGCAGGTG CAGGCGGACC TGATTCACAG CTGCCTGCAC 
CGTGTCATGG CCGTGCTGCC GGAGCCAGAG GAGGGGGACA GCCCCCAGGA GGTATCAGCG 
GCCCCCACAG TGGGGGGGGA CCCCCAGGAG CTTCTCTTGT TCCAGTCCCT GTACCTGGAC 
ACCGTGCAGG TCCTCAAGGA CTTGCTGACG AGCCTTCTTC TGTGGAACAT GACGCCCCTG 
GGTCTGCAGG TCATGTTGGA GCACCTGAGC CCATGGATCA AGTCCCCGAG GGGCCACGAG 
CGGGTGCGGG CGGTCGGCCT GAGTGCCTGC CTGCTGCAGT TCTTCCTTCA ACACCTGCAG 
ATCAGCGCCC TGGTGCCCTT CCACAACCTG GGCCTCCTCA TTGGCCTCTT CTCCCCACGG 
TGCGCCGACC TCTGGCCTGC CACTCGCCGA GAGGCTGTGA GCTGCATCCA CTCCCTGCTA 
TACCTGCAGC TGACCTGTGT GGGCTTCTCG CGAGACTACC AGGACGACGT GGCTGAGCAG 
CTCCTCAGCC TCAAAGATGG CCTGGTGCAC CCTGACCCTG CCATTCTCTT CCATACCTGC 
CACAGCATTG CCCAGCTTAT TGCCAAGCGC CTCCCTCCAG ATCAGCTCAT CAACCTCTTG 
CTAACCTTGT TTGAGAGCCT GGGAGATCCC GACAAGAACT GCTCGAGAGC TGCCAGCGTC 
ATGATCAACT GCCTGCTGAA GGAACGGGGC AACATGCTGC AGGAGAAGGT GCCCGAGATT 
GTGAGTGTGC TGCGCTCCAA GCTGCAGGAG ACCCAAGAGG AGCACATCCT ACAGGCCGCA 
CAGCACAGCG TGTTTGCCCT GGCCACCCAC CACTGTGCCT CTGTGGTGTC CAACCTTCTG 
GGCAGCCCCC TGCCCTTTGA CAGCCACACC TGCACCCTGT GGCGAGCACT GGCCTTGGAG 
CCCGGCCTCG CTGCACAGGT CCTGGGGCTG CTCCTGGAGA AGATAAGCAA GGACGTCCTG 
TTTGAGGAGA GCCAGGCCTT CCTGCTGAGC AGCACGCCGG ACCGCGTGGC CACCCTGCTG 
CCCCTCGCAG CCACCTGTGC ACTGCACGAG GTCGCATCTG CCCCGGCGTC CGGGCCAGCA 
GTGCTGGAGC TCTACCCCCA GCTGTTTGTG GCACTGCTGC TGCGGGTCAG CTGCACCGTG 
GGTGTCCAGC CGCCCAGGCA GCTGCAGGCC AAGGAGAGGA GGAGCGCCAG CTCGGGCCTG 
GCCTCACGGA GCCTCGAGCC TTGCAGCTCT GCGGTGGACG CGCTGCAGGC CGTGCTTGTC 
CGCGGTGGCA ATGAGGATGT GGTCCAGTGC ATGGAGCTGG ACGGGGGCTG GCAGTTGCTC 
AGGACCTCGG CTGGGCACGA GGAAGGTGTC ACCCGGCTGG CCAGTGCCAT GGCAAAGTTC 
GCAGGCCCCC GGCTGCCCCT GGTGATGAAG CTGCTCTTCA CCACACAGAG TAGCATGTAT 
GAGGTCCAGA GGGTCACCTC CACAGCCTTC CTGGCTGAGC TGCTCAGCAG CAATGTGGTG 
AACGACCTGA TGCTCCTGGA GTCACTGCTG TACAACCTGA TGGCACGGCA GAAGGACACG 
AGCGCCCGCG TGCGGAGGCT GGTGCTCCAC GGCCTGGCCA ACATCACCTT GGGCTCCCCA 
GATAAGGTAC AGACCCACAG CCCCCAACTC CTGACAGCCA TGATCGGTGG GCTGGACGAC 
GGGGACGACC CACACAGCCT GGTGGCGCTG GAGGCCATGG TGGGCCTGGC AAGGCTGATG 
GACCTGGTGG ACGCCTGGGA CCTACATGCA GTGCTGCTGC ACATTGCTGT CCGCATTCGG 
CCCTTCTTCG ACAGCGAAAG GATGGAGTTA CGCTCAGTGT CCATTGGCCT CTTCGGGCAC 
CTCAACAAGG CCTGCCGTGG GGACTGCAAG GACGTGTTCC TGGAGCAGGT TGTGGGCGGC 
CTGGTGCCCC TTCTGCTGCA CCTGCGTGAC CCCCACGCAC CCGTGGTCAC GGCCTGCAGG 
TTCGCCTTGC GCATGTGCGG CCCCAACCTG GAGTGTGAGG AGCTGGCAGC TGTCTTCCAG 
AGGCACCTGC AGGAAGGCCA TGACCTGCAC TTTGGAGAGT TCCTCAACAC CACCTGCAAG 
CACCTGATGC GCCACTTCCC CGACCTGCTG GGCCGCTTGC TGAGCACCAG TCTGTTCTAC 
TATAAGAGCA GCTGGGAGGA TGTCCGTGCT GCTGCCCCCA TGCTCACAGG GTTCTTGGTG 
CTGCACATGG AGGCTGAGCA GCGGCCACAG GTGGACCTGG AGCAGCTCCT CACGGCACTC 
CAGCTGCTGC TCAAGGACCC AGCCCTTAAG GTGCGCTTGA AGGCTGTGAA GACTCTGGGC 
CGCTTGGTGA AGTTCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810144.1
CDS	163..5223
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811842.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811842.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGACC CGGCCCTGAG ACTGAGCCTG 
GTGCAAAGTG TGTGCATGGC CACCCAGGCC ATCTGCAGCA GTGCCCACAG CAGCTCCTTC 
CACCTCTCGA GGAAGGCGGA GCTGGTGGCG CAGATGGTGG AGTTCATCAG AGCGGAGCCC 
CCAGACTCGC TGAAGACGCC CATTTGGAAG AAGGCCATGC TTGCCTGCAC GTACCTGGTT 
ACCCTGGAGC CGGCCCTGGA GGAGCAGGTG CAGGCGGACC TGATTCACAG CTGCCTGCAC 
CGTGTCATGG CCGTGCTGCC GGAGCCAGAG GAGGGGGACA GCCCCCAGGA GGTATCAGCG 
GCCCCCACAG TGGGGGGGGA CCCCCAGGAG CTTCTCTTGT TCCAGTCCCT GTACCTGGAC 
ACCGTGCAGG TCCTCAAGGA CTTGCTGACG AGCCTTCTTC TGTGGAACAT GACGCCCCTG 
GGTCTGCAGG TCATGTTGGA GCACCTGAGC CCATGGATCA AGTCCCCGAG GGGCCACGAG 
CGGGTGCGGG CGGTCGGCCT GAGTGCCTGC CTGCTGCAGT TCTTCCTTCA ACACCTGCAG 
ATCAGCGCCC TGGTGCCCTT CCACAACCTG GGCCTCCTCA TTGGCCTCTT CTCCCCACGG 
TGCGCCGACC TCTGGCCTGC CACTCGCCGA GAGGCTGTGA GCTGCATCCA CTCCCTGCTA 
TACCTGCAGC TGACCTGTGT GGGCTTCTCG CGAGACTACC AGGACGACGT GGCTGAGCAG 
CTCCTCAGCC TCAAAGATGG CCTGGTGCAC CCTGACCCTG CCATTCTCTT CCATACCTGC 
CACAGCATTG CCCAGCTTAT TGCCAAGCGC CTCCCTCCAG ATCAGCTCAT CAACCTCTTG 
CTAACCTTGT TTGAGAGCCT GGGAGATCCC GACAAGAACT GCTCGAGAGC TGCCAGCGTC 
ATGATCAACT GCCTGCTGAA GGAACGGGGC AACATGCTGC AGGAGAAGGT GCCCGAGATT 
GTGAGTGTGC TGCGCTCCAA GCTGCAGGAG ACCCAAGAGG AGCACATCCT ACAGGCCGCA 
CAGCACAGCG TGTTTGCCCT GGCCACCCAC CACTGTGCCT CTGTGGTGTC CAACCTTCTG 
GGCAGCCCCC TGCCCTTTGA CAGCCACACC TGCACCCTGT GGCGAGCACT GGCCTTGGAG 
CCCGGCCTCG CTGCACAGGT CCTGGGGCTG CTCCTGGAGA AGATAAGCAA GGACGTCCTG 
TTTGAGGAGA GCCAGGCCTT CCTGCTGAGC AGCACGCCGG ACCGCGTGGC CACCCTGCTG 
CCCCTCGCAG CCACCTGTGC ACTGCACGAG GTCGCATCTG CCCCGGCGTC CGGGCCAGCA 
GTGCTGGAGC TCTACCCCCA GCTGTTTGTG GCACTGCTGC TGCGGGTCAG CTGCACCGTG 
GGTGTCCAGC CGCCCAGGCA GCTGCAGGCC AAGGAGAGGA GGAGCGCCAG CTCGGGCCTG 
GCCTCACGGA GCCTCGAGCC TTGCAGCTCT GCGGTGGACG CGCTGCAGGC CGTGCTTGTC 
CGCGGTGGCA ATGAGGATGT GGTCCAGTGC ATGGAGCTGG ACGGGGGCTG GCAGTTGCTC 
AGGACCTCGG CTGGGCACGA GGAAGGTGTC ACCCGGCTGG CCAGTGCCAT GGCAAAGTTC 
GCAGGCCCCC GGCTGCCCCT GGTGATGAAG CTGCTCTTCA CCACACAGAG TAGCATGTAT 
GAGGTCCAGA GGGTCACCTC CACAGCCTTC CTGGCTGAGC TGCTCAGCAG CAATGTGGTG 
AACGACCTGA TGCTCCTGGA GTCACTGCTG TACAACCTGA TGGCACGGCA GAAGGACACG 
AGCGCCCGCG TGCGGAGGCT GGTGCTCCAC GGCCTGGCCA ACATCACCTT GGGCTCCCCA 
GATAAGGTAC AGACCCACAG CCCCCAACTC CTGACAGCCA TGATCGGTGG GCTGGACGAC 
GGGGACGACC CACACAGCCT GGTGGCGCTG GAGGCCATGG TGGGCCTGGC AAGGCTGATG 
GACCTGGTGG ACGCCTGGGA CCTACATGCA GTGCTGCTGC ACATTGCTGT CCGCATTCGG 
CCCTTCTTCG ACAGCGAAAG GATGGAGTTA CGCTCAGTGT CCATTGGCCT CTTCGGGCAC 
CTCAACAAGG CCTGCCGTGG GGACTGCAAG GACGTGTTCC TGGAGCAGGT TGTGGGCGGC 
CTGGTGCCCC TTCTGCTGCA CCTGCGTGAC CCCCACGCAC CCGTGGTCAC GGCCTGCAGG 
TTCGCCTTGC GCATGTGCGG CCCCAACCTG GAGTGTGAGG AGCTGGCAGC TGTCTTCCAG 
AGGCACCTGC AGGAAGGCCA TGACCTGCAC TTTGGAGAGT TCCTCAACAC CACCTGCAAG 
CACCTGATGC GCCACTTCCC CGACCTGCTG GGCCGCTTGC TGAGCACCAG TCTGTTCTAC 
TATAAGAGCA GCTGGGAGGA TGTCCGTGCT GCTGCCCCCA TGCTCACAGG GTTCTTGGTG 
CTGCACATGG AGGCTGAGCA GCGGCCACAG GTGGACCTGG AGCAGCTCCT CACGGCACTC 
CAGCTGCTGC TCAAGGACCC AGCCCTTAAG GTGCGCTTGA AGGCTGTGAA GACTCTGGGC 
CGCTTGGTGA AGTTCGCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811843.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810145.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.

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CloneID	OBa49180
Clone ID Related Accession (Same CDS sequence)	XM_010811843.2
Accession Version	XM_010811843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGCT TCTCTTGTTC CAGTCCCTGT ACCTGGACAC CGTGCAGGTC 
CTCAAGGACT TGCTGACGAG CCTTCTTCTG TGGAACATGA CGCCCCTGGG TCTGCAGGTC 
ATGTTGGAGC ACCTGAGCCC ATGGATCAAG TCCCCGAGGG GCCACGAGCG GGTGCGGGCG 
GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG 
GTGCCCTTCC ACAACCTGGG CCTCCTCATT GGCCTCTTCT CCCCACGGTG CGCCGACCTC 
TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC TGCATCCACT CCCTGCTATA CCTGCAGCTG 
ACCTGTGTGG GCTTCTCGCG AGACTACCAG GACGACGTGG CTGAGCAGCT CCTCAGCCTC 
AAAGATGGCC TGGTGCACCC TGACCCTGCC ATTCTCTTCC ATACCTGCCA CAGCATTGCC 
CAGCTTATTG CCAAGCGCCT CCCTCCAGAT CAGCTCATCA ACCTCTTGCT AACCTTGTTT 
GAGAGCCTGG GAGATCCCGA CAAGAACTGC TCGAGAGCTG CCAGCGTCAT GATCAACTGC 
CTGCTGAAGG AACGGGGCAA CATGCTGCAG GAGAAGGTGC CCGAGATTGT GAGTGTGCTG 
CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG CACATCCTAC AGGCCGCACA GCACAGCGTG 
TTTGCCCTGG CCACCCACCA CTGTGCCTCT GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG 
CCCTTTGACA GCCACACCTG CACCCTGTGG CGAGCACTGG CCTTGGAGCC CGGCCTCGCT 
GCACAGGTCC TGGGGCTGCT CCTGGAGAAG ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC 
CAGGCCTTCC TGCTGAGCAG CACGCCGGAC CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC 
ACCTGTGCAC TGCACGAGGT CGCATCTGCC CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC 
TACCCCCAGC TGTTTGTGGC ACTGCTGCTG CGGGTCAGCT GCACCGTGGG TGTCCAGCCG 
CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG AGCGCCAGCT CGGGCCTGGC CTCACGGAGC 
CTCGAGCCTT GCAGCTCTGC GGTGGACGCG CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT 
GAGGATGTGG TCCAGTGCAT GGAGCTGGAC GGGGGCTGGC AGTTGCTCAG GACCTCGGCT 
GGGCACGAGG AAGGTGTCAC CCGGCTGGCC AGTGCCATGG CAAAGTTCGC AGGCCCCCGG 
CTGCCCCTGG TGATGAAGCT GCTCTTCACC ACACAGAGTA GCATGTATGA GGTCCAGAGG 
GTCACCTCCA CAGCCTTCCT GGCTGAGCTG CTCAGCAGCA ATGTGGTGAA CGACCTGATG 
CTCCTGGAGT CACTGCTGTA CAACCTGATG GCACGGCAGA AGGACACGAG CGCCCGCGTG 
CGGAGGCTGG TGCTCCACGG CCTGGCCAAC ATCACCTTGG GCTCCCCAGA TAAGGTACAG 
ACCCACAGCC CCCAACTCCT GACAGCCATG ATCGGTGGGC TGGACGACGG GGACGACCCA 
CACAGCCTGG TGGCGCTGGA GGCCATGGTG GGCCTGGCAA GGCTGATGGA CCTGGTGGAC 
GCCTGGGACC TACATGCAGT GCTGCTGCAC ATTGCTGTCC GCATTCGGCC CTTCTTCGAC 
AGCGAAAGGA TGGAGTTACG CTCAGTGTCC ATTGGCCTCT TCGGGCACCT CAACAAGGCC 
TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT 
CTGCTGCACC TGCGTGACCC CCACGCACCC GTGGTCACGG CCTGCAGGTT CGCCTTGCGC 
ATGTGCGGCC CCAACCTGGA GTGTGAGGAG CTGGCAGCTG TCTTCCAGAG GCACCTGCAG 
GAAGGCCATG ACCTGCACTT TGGAGAGTTC CTCAACACCA CCTGCAAGCA CCTGATGCGC 
CACTTCCCCG ACCTGCTGGG CCGCTTGCTG AGCACCAGTC TGTTCTACTA TAAGAGCAGC 
TGGGAGGATG TCCGTGCTGC TGCCCCCATG CTCACAGGGT TCTTGGTGCT GCACATGGAG 
GCTGAGCAGC GGCCACAGGT GGACCTGGAG CAGCTCCTCA CGGCACTCCA GCTGCTGCTC 
AAGGACCCAG CCCTTAAGGT GCGCTTGAAG GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG 
TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810145.1
CDS	163..5211
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811843.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811843.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGCT TCTCTTGTTC CAGTCCCTGT ACCTGGACAC CGTGCAGGTC 
CTCAAGGACT TGCTGACGAG CCTTCTTCTG TGGAACATGA CGCCCCTGGG TCTGCAGGTC 
ATGTTGGAGC ACCTGAGCCC ATGGATCAAG TCCCCGAGGG GCCACGAGCG GGTGCGGGCG 
GTCGGCCTGA GTGCCTGCCT GCTGCAGTTC TTCCTTCAAC ACCTGCAGAT CAGCGCCCTG 
GTGCCCTTCC ACAACCTGGG CCTCCTCATT GGCCTCTTCT CCCCACGGTG CGCCGACCTC 
TGGCCTGCCA CTCGCCGAGA GGCTGTGAGC TGCATCCACT CCCTGCTATA CCTGCAGCTG 
ACCTGTGTGG GCTTCTCGCG AGACTACCAG GACGACGTGG CTGAGCAGCT CCTCAGCCTC 
AAAGATGGCC TGGTGCACCC TGACCCTGCC ATTCTCTTCC ATACCTGCCA CAGCATTGCC 
CAGCTTATTG CCAAGCGCCT CCCTCCAGAT CAGCTCATCA ACCTCTTGCT AACCTTGTTT 
GAGAGCCTGG GAGATCCCGA CAAGAACTGC TCGAGAGCTG CCAGCGTCAT GATCAACTGC 
CTGCTGAAGG AACGGGGCAA CATGCTGCAG GAGAAGGTGC CCGAGATTGT GAGTGTGCTG 
CGCTCCAAGC TGCAGGAGAC CCAAGAGGAG CACATCCTAC AGGCCGCACA GCACAGCGTG 
TTTGCCCTGG CCACCCACCA CTGTGCCTCT GTGGTGTCCA ACCTTCTGGG CAGCCCCCTG 
CCCTTTGACA GCCACACCTG CACCCTGTGG CGAGCACTGG CCTTGGAGCC CGGCCTCGCT 
GCACAGGTCC TGGGGCTGCT CCTGGAGAAG ATAAGCAAGG ACGTCCTGTT TGAGGAGAGC 
CAGGCCTTCC TGCTGAGCAG CACGCCGGAC CGCGTGGCCA CCCTGCTGCC CCTCGCAGCC 
ACCTGTGCAC TGCACGAGGT CGCATCTGCC CCGGCGTCCG GGCCAGCAGT GCTGGAGCTC 
TACCCCCAGC TGTTTGTGGC ACTGCTGCTG CGGGTCAGCT GCACCGTGGG TGTCCAGCCG 
CCCAGGCAGC TGCAGGCCAA GGAGAGGAGG AGCGCCAGCT CGGGCCTGGC CTCACGGAGC 
CTCGAGCCTT GCAGCTCTGC GGTGGACGCG CTGCAGGCCG TGCTTGTCCG CGGTGGCAAT 
GAGGATGTGG TCCAGTGCAT GGAGCTGGAC GGGGGCTGGC AGTTGCTCAG GACCTCGGCT 
GGGCACGAGG AAGGTGTCAC CCGGCTGGCC AGTGCCATGG CAAAGTTCGC AGGCCCCCGG 
CTGCCCCTGG TGATGAAGCT GCTCTTCACC ACACAGAGTA GCATGTATGA GGTCCAGAGG 
GTCACCTCCA CAGCCTTCCT GGCTGAGCTG CTCAGCAGCA ATGTGGTGAA CGACCTGATG 
CTCCTGGAGT CACTGCTGTA CAACCTGATG GCACGGCAGA AGGACACGAG CGCCCGCGTG 
CGGAGGCTGG TGCTCCACGG CCTGGCCAAC ATCACCTTGG GCTCCCCAGA TAAGGTACAG 
ACCCACAGCC CCCAACTCCT GACAGCCATG ATCGGTGGGC TGGACGACGG GGACGACCCA 
CACAGCCTGG TGGCGCTGGA GGCCATGGTG GGCCTGGCAA GGCTGATGGA CCTGGTGGAC 
GCCTGGGACC TACATGCAGT GCTGCTGCAC ATTGCTGTCC GCATTCGGCC CTTCTTCGAC 
AGCGAAAGGA TGGAGTTACG CTCAGTGTCC ATTGGCCTCT TCGGGCACCT CAACAAGGCC 
TGCCGTGGGG ACTGCAAGGA CGTGTTCCTG GAGCAGGTTG TGGGCGGCCT GGTGCCCCTT 
CTGCTGCACC TGCGTGACCC CCACGCACCC GTGGTCACGG CCTGCAGGTT CGCCTTGCGC 
ATGTGCGGCC CCAACCTGGA GTGTGAGGAG CTGGCAGCTG TCTTCCAGAG GCACCTGCAG 
GAAGGCCATG ACCTGCACTT TGGAGAGTTC CTCAACACCA CCTGCAAGCA CCTGATGCGC 
CACTTCCCCG ACCTGCTGGG CCGCTTGCTG AGCACCAGTC TGTTCTACTA TAAGAGCAGC 
TGGGAGGATG TCCGTGCTGC TGCCCCCATG CTCACAGGGT TCTTGGTGCT GCACATGGAG 
GCTGAGCAGC GGCCACAGGT GGACCTGGAG CAGCTCCTCA CGGCACTCCA GCTGCTGCTC 
AAGGACCCAG CCCTTAAGGT GCGCTTGAAG GCTGTGAAGA CTCTGGGCCG CTTGGTGAAG 
TTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811844.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810146.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.

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CloneID	OBa49181
Clone ID Related Accession (Same CDS sequence)	XM_010811844.2
Accession Version	XM_010811844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGCTTCTCT TGTTCCAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810146.1
CDS	163..5196
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811844.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811844.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGGTG AGAGAAGAGG AGAAGACGAG AGTTAGCCTG 
CTTTGGGAAC AGGAGGAGAA GACGAGATTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGGTATCA GCGGCCCCCA CAGTGGGGGG GGACCCCCAG 
GAGCTTCTCT TGTTCCAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811845.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810147.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.

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CloneID	OBa49182
Clone ID Related Accession (Same CDS sequence)	XM_010811845.2
Accession Version	XM_010811845.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811845.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810147.1
CDS	163..5196
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811845.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811845.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGTC CCTGTACCTG GACACCGTGC AGGTCCTCAA GGACTTGCTG 
ACGAGCCTTC TTCTGTGGAA CATGACGCCC CTGGGTCTGC AGGTCATGTT GGAGCACCTG 
AGCCCATGGA TCAAGTCCCC GAGGGGCCAC GAGCGGGTGC GGGCGGTCGG CCTGAGTGCC 
TGCCTGCTGC AGTTCTTCCT TCAACACCTG CAGATCAGCG CCCTGGTGCC CTTCCACAAC 
CTGGGCCTCC TCATTGGCCT CTTCTCCCCA CGGTGCGCCG ACCTCTGGCC TGCCACTCGC 
CGAGAGGCTG TGAGCTGCAT CCACTCCCTG CTATACCTGC AGCTGACCTG TGTGGGCTTC 
TCGCGAGACT ACCAGGACGA CGTGGCTGAG CAGCTCCTCA GCCTCAAAGA TGGCCTGGTG 
CACCCTGACC CTGCCATTCT CTTCCATACC TGCCACAGCA TTGCCCAGCT TATTGCCAAG 
CGCCTCCCTC CAGATCAGCT CATCAACCTC TTGCTAACCT TGTTTGAGAG CCTGGGAGAT 
CCCGACAAGA ACTGCTCGAG AGCTGCCAGC GTCATGATCA ACTGCCTGCT GAAGGAACGG 
GGCAACATGC TGCAGGAGAA GGTGCCCGAG ATTGTGAGTG TGCTGCGCTC CAAGCTGCAG 
GAGACCCAAG AGGAGCACAT CCTACAGGCC GCACAGCACA GCGTGTTTGC CCTGGCCACC 
CACCACTGTG CCTCTGTGGT GTCCAACCTT CTGGGCAGCC CCCTGCCCTT TGACAGCCAC 
ACCTGCACCC TGTGGCGAGC ACTGGCCTTG GAGCCCGGCC TCGCTGCACA GGTCCTGGGG 
CTGCTCCTGG AGAAGATAAG CAAGGACGTC CTGTTTGAGG AGAGCCAGGC CTTCCTGCTG 
AGCAGCACGC CGGACCGCGT GGCCACCCTG CTGCCCCTCG CAGCCACCTG TGCACTGCAC 
GAGGTCGCAT CTGCCCCGGC GTCCGGGCCA GCAGTGCTGG AGCTCTACCC CCAGCTGTTT 
GTGGCACTGC TGCTGCGGGT CAGCTGCACC GTGGGTGTCC AGCCGCCCAG GCAGCTGCAG 
GCCAAGGAGA GGAGGAGCGC CAGCTCGGGC CTGGCCTCAC GGAGCCTCGA GCCTTGCAGC 
TCTGCGGTGG ACGCGCTGCA GGCCGTGCTT GTCCGCGGTG GCAATGAGGA TGTGGTCCAG 
TGCATGGAGC TGGACGGGGG CTGGCAGTTG CTCAGGACCT CGGCTGGGCA CGAGGAAGGT 
GTCACCCGGC TGGCCAGTGC CATGGCAAAG TTCGCAGGCC CCCGGCTGCC CCTGGTGATG 
AAGCTGCTCT TCACCACACA GAGTAGCATG TATGAGGTCC AGAGGGTCAC CTCCACAGCC 
TTCCTGGCTG AGCTGCTCAG CAGCAATGTG GTGAACGACC TGATGCTCCT GGAGTCACTG 
CTGTACAACC TGATGGCACG GCAGAAGGAC ACGAGCGCCC GCGTGCGGAG GCTGGTGCTC 
CACGGCCTGG CCAACATCAC CTTGGGCTCC CCAGATAAGG TACAGACCCA CAGCCCCCAA 
CTCCTGACAG CCATGATCGG TGGGCTGGAC GACGGGGACG ACCCACACAG CCTGGTGGCG 
CTGGAGGCCA TGGTGGGCCT GGCAAGGCTG ATGGACCTGG TGGACGCCTG GGACCTACAT 
GCAGTGCTGC TGCACATTGC TGTCCGCATT CGGCCCTTCT TCGACAGCGA AAGGATGGAG 
TTACGCTCAG TGTCCATTGG CCTCTTCGGG CACCTCAACA AGGCCTGCCG TGGGGACTGC 
AAGGACGTGT TCCTGGAGCA GGTTGTGGGC GGCCTGGTGC CCCTTCTGCT GCACCTGCGT 
GACCCCCACG CACCCGTGGT CACGGCCTGC AGGTTCGCCT TGCGCATGTG CGGCCCCAAC 
CTGGAGTGTG AGGAGCTGGC AGCTGTCTTC CAGAGGCACC TGCAGGAAGG CCATGACCTG 
CACTTTGGAG AGTTCCTCAA CACCACCTGC AAGCACCTGA TGCGCCACTT CCCCGACCTG 
CTGGGCCGCT TGCTGAGCAC CAGTCTGTTC TACTATAAGA GCAGCTGGGA GGATGTCCGT 
GCTGCTGCCC CCATGCTCAC AGGGTTCTTG GTGCTGCACA TGGAGGCTGA GCAGCGGCCA 
CAGGTGGACC TGGAGCAGCT CCTCACGGCA CTCCAGCTGC TGCTCAAGGA CCCAGCCCTT 
AAGGTGCGCT TGAAGGCTGT GAAGACTCTG GGCCGCTTGG TGAAGTTCGC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811846.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810148.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.

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CloneID	OBa49183
Clone ID Related Accession (Same CDS sequence)	XM_010811846.2
Accession Version	XM_010811846.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5028bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811846.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GTTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810148.1
CDS	163..5190
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_010811846.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811846.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GTTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGCTGC TCAGCAGCAA TGTGGTGAAC GACCTGATGC TCCTGGAGTC ACTGCTGTAC 
AACCTGATGG CACGGCAGAA GGACACGAGC GCCCGCGTGC GGAGGCTGGT GCTCCACGGC 
CTGGCCAACA TCACCTTGGG CTCCCCAGAT AAGGTACAGA CCCACAGCCC CCAACTCCTG 
ACAGCCATGA TCGGTGGGCT GGACGACGGG GACGACCCAC ACAGCCTGGT GGCGCTGGAG 
GCCATGGTGG GCCTGGCAAG GCTGATGGAC CTGGTGGACG CCTGGGACCT ACATGCAGTG 
CTGCTGCACA TTGCTGTCCG CATTCGGCCC TTCTTCGACA GCGAAAGGAT GGAGTTACGC 
TCAGTGTCCA TTGGCCTCTT CGGGCACCTC AACAAGGCCT GCCGTGGGGA CTGCAAGGAC 
GTGTTCCTGG AGCAGGTTGT GGGCGGCCTG GTGCCCCTTC TGCTGCACCT GCGTGACCCC 
CACGCACCCG TGGTCACGGC CTGCAGGTTC GCCTTGCGCA TGTGCGGCCC CAACCTGGAG 
TGTGAGGAGC TGGCAGCTGT CTTCCAGAGG CACCTGCAGG AAGGCCATGA CCTGCACTTT 
GGAGAGTTCC TCAACACCAC CTGCAAGCAC CTGATGCGCC ACTTCCCCGA CCTGCTGGGC 
CGCTTGCTGA GCACCAGTCT GTTCTACTAT AAGAGCAGCT GGGAGGATGT CCGTGCTGCT 
GCCCCCATGC TCACAGGGTT CTTGGTGCTG CACATGGAGG CTGAGCAGCG GCCACAGGTG 
GACCTGGAGC AGCTCCTCAC GGCACTCCAG CTGCTGCTCA AGGACCCAGC CCTTAAGGTG 
CGCTTGAAGG CTGTGAAGAC TCTGGGCCGC TTGGTGAAGT TCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_005215189.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215246.2 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.

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CloneID	OBa49184
Clone ID Related Accession (Same CDS sequence)	XM_005215189.3 , XM_005215189.4
Accession Version	XM_005215189.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGTCCCTG TACCTGGACA CCGTGCAGGT CCTCAAGGAC 
TTGCTGACGA GCCTTCTTCT GTGGAACATG ACGCCCCTGG GTCTGCAGGT CATGTTGGAG 
CACCTGAGCC CATGGATCAA GTCCCCGAGG GGCCACGAGC GGGTGCGGGC GGTCGGCCTG 
AGTGCCTGCC TGCTGCAGTT CTTCCTTCAA CACCTGCAGA TCAGCGCCCT GGTGCCCTTC 
CACAACCTGG GCCTCCTCAT TGGCCTCTTC TCCCCACGGT GCGCCGACCT CTGGCCTGCC 
ACTCGCCGAG AGGCTGTGAG CTGCATCCAC TCCCTGCTAT ACCTGCAGCT GACCTGTGTG 
GGCTTCTCGC GAGACTACCA GGACGACGTG GCTGAGCAGC TCCTCAGCCT CAAAGATGGC 
CTGGTGCACC CTGACCCTGC CATTCTCTTC CATACCTGCC ACAGCATTGC CCAGCTTATT 
GCCAAGCGCC TCCCTCCAGA TCAGCTCATC AACCTCTTGC TAACCTTGTT TGAGAGCCTG 
GGAGATCCCG ACAAGAACTG CTCGAGAGCT GCCAGCGTCA TGATCAACTG CCTGCTGAAG 
GAACGGGGCA ACATGCTGCA GGAGAAGGTG CCCGAGATTG TGAGTGTGCT GCGCTCCAAG 
CTGCAGGAGA CCCAAGAGGA GCACATCCTA CAGGCCGCAC AGCACAGCGT GTTTGCCCTG 
GCCACCCACC ACTGTGCCTC TGTGGTGTCC AACCTTCTGG GCAGCCCCCT GCCCTTTGAC 
AGCCACACCT GCACCCTGTG GCGAGCACTG GCCTTGGAGC CCGGCCTCGC TGCACAGGTC 
CTGGGGCTGC TCCTGGAGAA GATAAGCAAG GACGTCCTGT TTGAGGAGAG CCAGGCCTTC 
CTGCTGAGCA GCACGCCGGA CCGCGTGGCC ACCCTGCTGC CCCTCGCAGC CACCTGTGCA 
CTGCACGAGG TCGCATCTGC CCCGGCGTCC GGGCCAGCAG TGCTGGAGCT CTACCCCCAG 
CTGTTTGTGG CACTGCTGCT GCGGGTCAGC TGCACCGTGG GTGTCCAGCC GCCCAGGCAG 
CTGCAGGCCA AGGAGAGGAG GAGCGCCAGC TCGGGCCTGG CCTCACGGAG CCTCGAGCCT 
TGCAGCTCTG CGGTGGACGC GCTGCAGGCC GTGCTTGTCC GCGGTGGCAA TGAGGATGTG 
GTCCAGTGCA TGGAGCTGGA CGGGGGCTGG CAGTTGCTCA GGACCTCGGC TGGGCACGAG 
GAAGGTGTCA CCCGGCTGGC CAGTGCCATG GCAAAGTTCG CAGGCCCCCG GCTGCCCCTG 
GTGATGAAGC TGCTCTTCAC CACACAGAGT AGCATGTATG AGGTCCAGAG GGTCACCTCC 
ACAGCCTTCC TGGCTGAGCT GCTCAGCAGC AATGTGGTGA ACGACCTGAT GCTCCTGGAG 
TCACTGCTGT ACAACCTGAT GGCACGGCAG AAGGACACGA GCGCCCGCGT GCGGAGGCTG 
GTGCTCCACG GCCTGGCCAA CATCACCTTG GGCTCCCCAG ATAAGGTACA GACCCACAGC 
CCCCAACTCC TGACAGCCAT GATCGGTGGG CTGGACGACG GGGACGACCC ACACAGCCTG 
GTGGCGCTGG AGGCCATGGT GGGCCTGGCA AGGCTGATGG ACCTGGTGGA CGCCTGGGAC 
CTACATGCAG TGCTGCTGCA CATTGCTGTC CGCATTCGGC CCTTCTTCGA CAGCGAAAGG 
ATGGAGTTAC GCTCAGTGTC CATTGGCCTC TTCGGGCACC TCAACAAGGC CTGCCGTGGG 
GACTGCAAGG ACGTGTTCCT GGAGCAGGTT GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC 
CTGCGTGACC CCCACGCACC CGTGGTCACG GCCTGCAGGT TCGCCTTGCG CATGTGCGGC 
CCCAACCTGG AGTGTGAGGA GCTGGCAGCT GTCTTCCAGA GGCACCTGCA GGAAGGCCAT 
GACCTGCACT TTGGAGAGTT CCTCAACACC ACCTGCAAGC ACCTGATGCG CCACTTCCCC 
GACCTGCTGG GCCGCTTGCT GAGCACCAGT CTGTTCTACT ATAAGAGCAG CTGGGAGGAT 
GTCCGTGCTG CTGCCCCCAT GCTCACAGGG TTCTTGGTGC TGCACATGGA GGCTGAGCAG 
CGGCCACAGG TGGACCTGGA GCAGCTCCTC ACGGCACTCC AGCTGCTGCT CAAGGACCCA 
GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215246.2
CDS	163..5082
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_005215189.3
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215189.3

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGTCCCTG TACCTGGACA CCGTGCAGGT CCTCAAGGAC 
TTGCTGACGA GCCTTCTTCT GTGGAACATG ACGCCCCTGG GTCTGCAGGT CATGTTGGAG 
CACCTGAGCC CATGGATCAA GTCCCCGAGG GGCCACGAGC GGGTGCGGGC GGTCGGCCTG 
AGTGCCTGCC TGCTGCAGTT CTTCCTTCAA CACCTGCAGA TCAGCGCCCT GGTGCCCTTC 
CACAACCTGG GCCTCCTCAT TGGCCTCTTC TCCCCACGGT GCGCCGACCT CTGGCCTGCC 
ACTCGCCGAG AGGCTGTGAG CTGCATCCAC TCCCTGCTAT ACCTGCAGCT GACCTGTGTG 
GGCTTCTCGC GAGACTACCA GGACGACGTG GCTGAGCAGC TCCTCAGCCT CAAAGATGGC 
CTGGTGCACC CTGACCCTGC CATTCTCTTC CATACCTGCC ACAGCATTGC CCAGCTTATT 
GCCAAGCGCC TCCCTCCAGA TCAGCTCATC AACCTCTTGC TAACCTTGTT TGAGAGCCTG 
GGAGATCCCG ACAAGAACTG CTCGAGAGCT GCCAGCGTCA TGATCAACTG CCTGCTGAAG 
GAACGGGGCA ACATGCTGCA GGAGAAGGTG CCCGAGATTG TGAGTGTGCT GCGCTCCAAG 
CTGCAGGAGA CCCAAGAGGA GCACATCCTA CAGGCCGCAC AGCACAGCGT GTTTGCCCTG 
GCCACCCACC ACTGTGCCTC TGTGGTGTCC AACCTTCTGG GCAGCCCCCT GCCCTTTGAC 
AGCCACACCT GCACCCTGTG GCGAGCACTG GCCTTGGAGC CCGGCCTCGC TGCACAGGTC 
CTGGGGCTGC TCCTGGAGAA GATAAGCAAG GACGTCCTGT TTGAGGAGAG CCAGGCCTTC 
CTGCTGAGCA GCACGCCGGA CCGCGTGGCC ACCCTGCTGC CCCTCGCAGC CACCTGTGCA 
CTGCACGAGG TCGCATCTGC CCCGGCGTCC GGGCCAGCAG TGCTGGAGCT CTACCCCCAG 
CTGTTTGTGG CACTGCTGCT GCGGGTCAGC TGCACCGTGG GTGTCCAGCC GCCCAGGCAG 
CTGCAGGCCA AGGAGAGGAG GAGCGCCAGC TCGGGCCTGG CCTCACGGAG CCTCGAGCCT 
TGCAGCTCTG CGGTGGACGC GCTGCAGGCC GTGCTTGTCC GCGGTGGCAA TGAGGATGTG 
GTCCAGTGCA TGGAGCTGGA CGGGGGCTGG CAGTTGCTCA GGACCTCGGC TGGGCACGAG 
GAAGGTGTCA CCCGGCTGGC CAGTGCCATG GCAAAGTTCG CAGGCCCCCG GCTGCCCCTG 
GTGATGAAGC TGCTCTTCAC CACACAGAGT AGCATGTATG AGGTCCAGAG GGTCACCTCC 
ACAGCCTTCC TGGCTGAGCT GCTCAGCAGC AATGTGGTGA ACGACCTGAT GCTCCTGGAG 
TCACTGCTGT ACAACCTGAT GGCACGGCAG AAGGACACGA GCGCCCGCGT GCGGAGGCTG 
GTGCTCCACG GCCTGGCCAA CATCACCTTG GGCTCCCCAG ATAAGGTACA GACCCACAGC 
CCCCAACTCC TGACAGCCAT GATCGGTGGG CTGGACGACG GGGACGACCC ACACAGCCTG 
GTGGCGCTGG AGGCCATGGT GGGCCTGGCA AGGCTGATGG ACCTGGTGGA CGCCTGGGAC 
CTACATGCAG TGCTGCTGCA CATTGCTGTC CGCATTCGGC CCTTCTTCGA CAGCGAAAGG 
ATGGAGTTAC GCTCAGTGTC CATTGGCCTC TTCGGGCACC TCAACAAGGC CTGCCGTGGG 
GACTGCAAGG ACGTGTTCCT GGAGCAGGTT GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC 
CTGCGTGACC CCCACGCACC CGTGGTCACG GCCTGCAGGT TCGCCTTGCG CATGTGCGGC 
CCCAACCTGG AGTGTGAGGA GCTGGCAGCT GTCTTCCAGA GGCACCTGCA GGAAGGCCAT 
GACCTGCACT TTGGAGAGTT CCTCAACACC ACCTGCAAGC ACCTGATGCG CCACTTCCCC 
GACCTGCTGG GCCGCTTGCT GAGCACCAGT CTGTTCTACT ATAAGAGCAG CTGGGAGGAT 
GTCCGTGCTG CTGCCCCCAT GCTCACAGGG TTCTTGGTGC TGCACATGGA GGCTGAGCAG 
CGGCCACAGG TGGACCTGGA GCAGCTCCTC ACGGCACTCC AGCTGCTGCT CAAGGACCCA 
GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_005215189.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215246.2 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa49184
Clone ID Related Accession (Same CDS sequence)	XM_005215189.3 , XM_005215189.4
Accession Version	XM_005215189.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4920bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215189.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGTCCCTG TACCTGGACA CCGTGCAGGT CCTCAAGGAC 
TTGCTGACGA GCCTTCTTCT GTGGAACATG ACGCCCCTGG GTCTGCAGGT CATGTTGGAG 
CACCTGAGCC CATGGATCAA GTCCCCGAGG GGCCACGAGC GGGTGCGGGC GGTCGGCCTG 
AGTGCCTGCC TGCTGCAGTT CTTCCTTCAA CACCTGCAGA TCAGCGCCCT GGTGCCCTTC 
CACAACCTGG GCCTCCTCAT TGGCCTCTTC TCCCCACGGT GCGCCGACCT CTGGCCTGCC 
ACTCGCCGAG AGGCTGTGAG CTGCATCCAC TCCCTGCTAT ACCTGCAGCT GACCTGTGTG 
GGCTTCTCGC GAGACTACCA GGACGACGTG GCTGAGCAGC TCCTCAGCCT CAAAGATGGC 
CTGGTGCACC CTGACCCTGC CATTCTCTTC CATACCTGCC ACAGCATTGC CCAGCTTATT 
GCCAAGCGCC TCCCTCCAGA TCAGCTCATC AACCTCTTGC TAACCTTGTT TGAGAGCCTG 
GGAGATCCCG ACAAGAACTG CTCGAGAGCT GCCAGCGTCA TGATCAACTG CCTGCTGAAG 
GAACGGGGCA ACATGCTGCA GGAGAAGGTG CCCGAGATTG TGAGTGTGCT GCGCTCCAAG 
CTGCAGGAGA CCCAAGAGGA GCACATCCTA CAGGCCGCAC AGCACAGCGT GTTTGCCCTG 
GCCACCCACC ACTGTGCCTC TGTGGTGTCC AACCTTCTGG GCAGCCCCCT GCCCTTTGAC 
AGCCACACCT GCACCCTGTG GCGAGCACTG GCCTTGGAGC CCGGCCTCGC TGCACAGGTC 
CTGGGGCTGC TCCTGGAGAA GATAAGCAAG GACGTCCTGT TTGAGGAGAG CCAGGCCTTC 
CTGCTGAGCA GCACGCCGGA CCGCGTGGCC ACCCTGCTGC CCCTCGCAGC CACCTGTGCA 
CTGCACGAGG TCGCATCTGC CCCGGCGTCC GGGCCAGCAG TGCTGGAGCT CTACCCCCAG 
CTGTTTGTGG CACTGCTGCT GCGGGTCAGC TGCACCGTGG GTGTCCAGCC GCCCAGGCAG 
CTGCAGGCCA AGGAGAGGAG GAGCGCCAGC TCGGGCCTGG CCTCACGGAG CCTCGAGCCT 
TGCAGCTCTG CGGTGGACGC GCTGCAGGCC GTGCTTGTCC GCGGTGGCAA TGAGGATGTG 
GTCCAGTGCA TGGAGCTGGA CGGGGGCTGG CAGTTGCTCA GGACCTCGGC TGGGCACGAG 
GAAGGTGTCA CCCGGCTGGC CAGTGCCATG GCAAAGTTCG CAGGCCCCCG GCTGCCCCTG 
GTGATGAAGC TGCTCTTCAC CACACAGAGT AGCATGTATG AGGTCCAGAG GGTCACCTCC 
ACAGCCTTCC TGGCTGAGCT GCTCAGCAGC AATGTGGTGA ACGACCTGAT GCTCCTGGAG 
TCACTGCTGT ACAACCTGAT GGCACGGCAG AAGGACACGA GCGCCCGCGT GCGGAGGCTG 
GTGCTCCACG GCCTGGCCAA CATCACCTTG GGCTCCCCAG ATAAGGTACA GACCCACAGC 
CCCCAACTCC TGACAGCCAT GATCGGTGGG CTGGACGACG GGGACGACCC ACACAGCCTG 
GTGGCGCTGG AGGCCATGGT GGGCCTGGCA AGGCTGATGG ACCTGGTGGA CGCCTGGGAC 
CTACATGCAG TGCTGCTGCA CATTGCTGTC CGCATTCGGC CCTTCTTCGA CAGCGAAAGG 
ATGGAGTTAC GCTCAGTGTC CATTGGCCTC TTCGGGCACC TCAACAAGGC CTGCCGTGGG 
GACTGCAAGG ACGTGTTCCT GGAGCAGGTT GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC 
CTGCGTGACC CCCACGCACC CGTGGTCACG GCCTGCAGGT TCGCCTTGCG CATGTGCGGC 
CCCAACCTGG AGTGTGAGGA GCTGGCAGCT GTCTTCCAGA GGCACCTGCA GGAAGGCCAT 
GACCTGCACT TTGGAGAGTT CCTCAACACC ACCTGCAAGC ACCTGATGCG CCACTTCCCC 
GACCTGCTGG GCCGCTTGCT GAGCACCAGT CTGTTCTACT ATAAGAGCAG CTGGGAGGAT 
GTCCGTGCTG CTGCCCCCAT GCTCACAGGG TTCTTGGTGC TGCACATGGA GGCTGAGCAG 
CGGCCACAGG TGGACCTGGA GCAGCTCCTC ACGGCACTCC AGCTGCTGCT CAAGGACCCA 
GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215246.2
CDS	161..5080
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_005215189.4
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215189.4

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGTTCTGGG GATAAAGGTA 
GAAACCCAGG ACCCGGCCCT GAGACTGAGC CTGGTGCAAA GTGTGTGCAT GGCCACCCAG 
GCCATCTGCA GCAGTGCCCA CAGCAGCTCC TTCCACCTCT CGAGGAAGGC GGAGCTGGTG 
GCGCAGATGG TGGAGTTCAT CAGAGCGGAG CCCCCAGACT CGCTGAAGAC GCCCATTTGG 
AAGAAGGCCA TGCTTGCCTG CACGTACCTG GTTACCCTGG AGCCGGCCCT GGAGGAGCAG 
GTGCAGGCGG ACCTGATTCA CAGCTGCCTG CACCGTGTCA TGGCCGTGCT GCCGGAGCCA 
GAGGAGGGGG ACAGCCCCCA GGAGTCCCTG TACCTGGACA CCGTGCAGGT CCTCAAGGAC 
TTGCTGACGA GCCTTCTTCT GTGGAACATG ACGCCCCTGG GTCTGCAGGT CATGTTGGAG 
CACCTGAGCC CATGGATCAA GTCCCCGAGG GGCCACGAGC GGGTGCGGGC GGTCGGCCTG 
AGTGCCTGCC TGCTGCAGTT CTTCCTTCAA CACCTGCAGA TCAGCGCCCT GGTGCCCTTC 
CACAACCTGG GCCTCCTCAT TGGCCTCTTC TCCCCACGGT GCGCCGACCT CTGGCCTGCC 
ACTCGCCGAG AGGCTGTGAG CTGCATCCAC TCCCTGCTAT ACCTGCAGCT GACCTGTGTG 
GGCTTCTCGC GAGACTACCA GGACGACGTG GCTGAGCAGC TCCTCAGCCT CAAAGATGGC 
CTGGTGCACC CTGACCCTGC CATTCTCTTC CATACCTGCC ACAGCATTGC CCAGCTTATT 
GCCAAGCGCC TCCCTCCAGA TCAGCTCATC AACCTCTTGC TAACCTTGTT TGAGAGCCTG 
GGAGATCCCG ACAAGAACTG CTCGAGAGCT GCCAGCGTCA TGATCAACTG CCTGCTGAAG 
GAACGGGGCA ACATGCTGCA GGAGAAGGTG CCCGAGATTG TGAGTGTGCT GCGCTCCAAG 
CTGCAGGAGA CCCAAGAGGA GCACATCCTA CAGGCCGCAC AGCACAGCGT GTTTGCCCTG 
GCCACCCACC ACTGTGCCTC TGTGGTGTCC AACCTTCTGG GCAGCCCCCT GCCCTTTGAC 
AGCCACACCT GCACCCTGTG GCGAGCACTG GCCTTGGAGC CCGGCCTCGC TGCACAGGTC 
CTGGGGCTGC TCCTGGAGAA GATAAGCAAG GACGTCCTGT TTGAGGAGAG CCAGGCCTTC 
CTGCTGAGCA GCACGCCGGA CCGCGTGGCC ACCCTGCTGC CCCTCGCAGC CACCTGTGCA 
CTGCACGAGG TCGCATCTGC CCCGGCGTCC GGGCCAGCAG TGCTGGAGCT CTACCCCCAG 
CTGTTTGTGG CACTGCTGCT GCGGGTCAGC TGCACCGTGG GTGTCCAGCC GCCCAGGCAG 
CTGCAGGCCA AGGAGAGGAG GAGCGCCAGC TCGGGCCTGG CCTCACGGAG CCTCGAGCCT 
TGCAGCTCTG CGGTGGACGC GCTGCAGGCC GTGCTTGTCC GCGGTGGCAA TGAGGATGTG 
GTCCAGTGCA TGGAGCTGGA CGGGGGCTGG CAGTTGCTCA GGACCTCGGC TGGGCACGAG 
GAAGGTGTCA CCCGGCTGGC CAGTGCCATG GCAAAGTTCG CAGGCCCCCG GCTGCCCCTG 
GTGATGAAGC TGCTCTTCAC CACACAGAGT AGCATGTATG AGGTCCAGAG GGTCACCTCC 
ACAGCCTTCC TGGCTGAGCT GCTCAGCAGC AATGTGGTGA ACGACCTGAT GCTCCTGGAG 
TCACTGCTGT ACAACCTGAT GGCACGGCAG AAGGACACGA GCGCCCGCGT GCGGAGGCTG 
GTGCTCCACG GCCTGGCCAA CATCACCTTG GGCTCCCCAG ATAAGGTACA GACCCACAGC 
CCCCAACTCC TGACAGCCAT GATCGGTGGG CTGGACGACG GGGACGACCC ACACAGCCTG 
GTGGCGCTGG AGGCCATGGT GGGCCTGGCA AGGCTGATGG ACCTGGTGGA CGCCTGGGAC 
CTACATGCAG TGCTGCTGCA CATTGCTGTC CGCATTCGGC CCTTCTTCGA CAGCGAAAGG 
ATGGAGTTAC GCTCAGTGTC CATTGGCCTC TTCGGGCACC TCAACAAGGC CTGCCGTGGG 
GACTGCAAGG ACGTGTTCCT GGAGCAGGTT GTGGGCGGCC TGGTGCCCCT TCTGCTGCAC 
CTGCGTGACC CCCACGCACC CGTGGTCACG GCCTGCAGGT TCGCCTTGCG CATGTGCGGC 
CCCAACCTGG AGTGTGAGGA GCTGGCAGCT GTCTTCCAGA GGCACCTGCA GGAAGGCCAT 
GACCTGCACT TTGGAGAGTT CCTCAACACC ACCTGCAAGC ACCTGATGCG CCACTTCCCC 
GACCTGCTGG GCCGCTTGCT GAGCACCAGT CTGTTCTACT ATAAGAGCAG CTGGGAGGAT 
GTCCGTGCTG CTGCCCCCAT GCTCACAGGG TTCTTGGTGC TGCACATGGA GGCTGAGCAG 
CGGCCACAGG TGGACCTGGA GCAGCTCCTC ACGGCACTCC AGCTGCTGCT CAAGGACCCA 
GCCCTTAAGG TGCGCTTGAA GGCTGTGAAG ACTCTGGGCC GCTTGGTGAA GTTCGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_005215190.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215247.2 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.

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CloneID	OBa49185
Clone ID Related Accession (Same CDS sequence)	XM_005215190.3 , XM_005215190.4
Accession Version	XM_005215190.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215190.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215247.2
CDS	163..5055
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_005215190.3
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215190.3

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_005215190.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005215247.2 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X17, mRNA.

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CloneID	OBa49185
Clone ID Related Accession (Same CDS sequence)	XM_005215190.3 , XM_005215190.4
Accession Version	XM_005215190.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005215190.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005215247.2
CDS	161..5053
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQESLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAELLSSNVVNDLMLLESLLYNLMARQKDTSARVRRLVLHGLANITLGSPDKVQTHSPQLLTAMIGGLDDGDDPHSLVALEAMVGLARLMDLVDAWDLHAVLLHIAVRIRPFFDSERMELRSVSIGLFGHLNKACRGDCKDVFLEQVVGGLVPLLLHLRDPHAPVVTACRFALRMCGPNLECEELAAVFQRHLQEGHDLHFGEFLNTTCKHLMRHFPDLLGRLLSTSLFYYKSSWEDVRAAAPMLTGFLVLHMEAEQRPQVDLEQLLTALQLLLKDPALKVRLKAVKTLGRLVKFA

Target ORF information:

RefSeq Version	XM_005215190.4
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005215190.4

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGCCGTGTC TTCCCATCCC TATGTGGGGG ACGGTCGCGG GGCAGCCTCG 
CTGCGCCTCT TGAATGTCTT GCATCAGGAC ATCCACCCGG CCCTGGGTCA GCGGTGGGTG 
ACCGCCATCC CCCTGCTGCT GGAGCACCTG GACGAATACA GTGAAGAAAC GCTGTCACAG 
AAGGAGTGGG AAGAAAAGCT GCTGGTGTTT CTCCGGGATT CCCTGGCTGT CGTGTCTGAC 
AACACCTGGG TCTGCCACCT GACCCTGGAA ATGTGCAAGC AGCTACCCAA CTACAACGGG 
ACGCCCCTGG AGAAGAACTT CCTGTACAAA TGTGTCGGGA CCACCCTGGG TGCCGCTTCA 
AGTAAGGTGG TGAGGAAGCA CCTGCGGGAG CTGCTGGAGA CGGCCCGATA CCAGGAGGAG 
AGGGAGCACG AGGGCCTTGC CTGTTGCTTT GGGATCTGTG CCATCTCCCA CCTGGATGAC 
ACCTTGGCCC AGCTGGACGA CTTTGTGAAG TCAGACGTAC TCAGAAAATC TGCTGGCATT 
TTCAACCTTT TTAAGAATCG AAGTGAGAGT GAGGCCAACA AAGTGAGGAG TGCGCTGATC 
CTGTGCTATG GGCATGTGGC GGCCGGGGCC CCGCGCGAGC TGCTGCTGGC CAGGGTGGAG 
GCGGACTTGT TCTGGAACAT GTCCGAGTGC TTCAGCACCA AGGACCCGGC CCTGAGACTG 
AGCCTGGTGC AAAGTGTGTG CATGGCCACC CAGGCCATCT GCAGCAGTGC CCACAGCAGC 
TCCTTCCACC TCTCGAGGAA GGCGGAGCTG GTGGCGCAGA TGGTGGAGTT CATCAGAGCG 
GAGCCCCCAG ACTCGCTGAA GACGCCCATT TGGAAGAAGG CCATGCTTGC CTGCACGTAC 
CTGGTTACCC TGGAGCCGGC CCTGGAGGAG CAGGTGCAGG CGGACCTGAT TCACAGCTGC 
CTGCACCGTG TCATGGCCGT GCTGCCGGAG CCAGAGGAGG GGGACAGCCC CCAGGAGTCC 
CTGTACCTGG ACACCGTGCA GGTCCTCAAG GACTTGCTGA CGAGCCTTCT TCTGTGGAAC 
ATGACGCCCC TGGGTCTGCA GGTCATGTTG GAGCACCTGA GCCCATGGAT CAAGTCCCCG 
AGGGGCCACG AGCGGGTGCG GGCGGTCGGC CTGAGTGCCT GCCTGCTGCA GTTCTTCCTT 
CAACACCTGC AGATCAGCGC CCTGGTGCCC TTCCACAACC TGGGCCTCCT CATTGGCCTC 
TTCTCCCCAC GGTGCGCCGA CCTCTGGCCT GCCACTCGCC GAGAGGCTGT GAGCTGCATC 
CACTCCCTGC TATACCTGCA GCTGACCTGT GTGGGCTTCT CGCGAGACTA CCAGGACGAC 
GTGGCTGAGC AGCTCCTCAG CCTCAAAGAT GGCCTGGTGC ACCCTGACCC TGCCATTCTC 
TTCCATACCT GCCACAGCAT TGCCCAGCTT ATTGCCAAGC GCCTCCCTCC AGATCAGCTC 
ATCAACCTCT TGCTAACCTT GTTTGAGAGC CTGGGAGATC CCGACAAGAA CTGCTCGAGA 
GCTGCCAGCG TCATGATCAA CTGCCTGCTG AAGGAACGGG GCAACATGCT GCAGGAGAAG 
GTGCCCGAGA TTGTGAGTGT GCTGCGCTCC AAGCTGCAGG AGACCCAAGA GGAGCACATC 
CTACAGGCCG CACAGCACAG CGTGTTTGCC CTGGCCACCC ACCACTGTGC CTCTGTGGTG 
TCCAACCTTC TGGGCAGCCC CCTGCCCTTT GACAGCCACA CCTGCACCCT GTGGCGAGCA 
CTGGCCTTGG AGCCCGGCCT CGCTGCACAG GTCCTGGGGC TGCTCCTGGA GAAGATAAGC 
AAGGACGTCC TGTTTGAGGA GAGCCAGGCC TTCCTGCTGA GCAGCACGCC GGACCGCGTG 
GCCACCCTGC TGCCCCTCGC AGCCACCTGT GCACTGCACG AGGTCGCATC TGCCCCGGCG 
TCCGGGCCAG CAGTGCTGGA GCTCTACCCC CAGCTGTTTG TGGCACTGCT GCTGCGGGTC 
AGCTGCACCG TGGGTGTCCA GCCGCCCAGG CAGCTGCAGG CCAAGGAGAG GAGGAGCGCC 
AGCTCGGGCC TGGCCTCACG GAGCCTCGAG CCTTGCAGCT CTGCGGTGGA CGCGCTGCAG 
GCCGTGCTTG TCCGCGGTGG CAATGAGGAT GTGGTCCAGT GCATGGAGCT GGACGGGGGC 
TGGCAGTTGC TCAGGACCTC GGCTGGGCAC GAGGAAGGTG TCACCCGGCT GGCCAGTGCC 
ATGGCAAAGT TCGCAGGCCC CCGGCTGCCC CTGGTGATGA AGCTGCTCTT CACCACACAG 
AGTAGCATGT ATGAGGTCCA GAGGGTCACC TCCACAGCCT TCCTGGCTGA GCTGCTCAGC 
AGCAATGTGG TGAACGACCT GATGCTCCTG GAGTCACTGC TGTACAACCT GATGGCACGG 
CAGAAGGACA CGAGCGCCCG CGTGCGGAGG CTGGTGCTCC ACGGCCTGGC CAACATCACC 
TTGGGCTCCC CAGATAAGGT ACAGACCCAC AGCCCCCAAC TCCTGACAGC CATGATCGGT 
GGGCTGGACG ACGGGGACGA CCCACACAGC CTGGTGGCGC TGGAGGCCAT GGTGGGCCTG 
GCAAGGCTGA TGGACCTGGT GGACGCCTGG GACCTACATG CAGTGCTGCT GCACATTGCT 
GTCCGCATTC GGCCCTTCTT CGACAGCGAA AGGATGGAGT TACGCTCAGT GTCCATTGGC 
CTCTTCGGGC ACCTCAACAA GGCCTGCCGT GGGGACTGCA AGGACGTGTT CCTGGAGCAG 
GTTGTGGGCG GCCTGGTGCC CCTTCTGCTG CACCTGCGTG ACCCCCACGC ACCCGTGGTC 
ACGGCCTGCA GGTTCGCCTT GCGCATGTGC GGCCCCAACC TGGAGTGTGA GGAGCTGGCA 
GCTGTCTTCC AGAGGCACCT GCAGGAAGGC CATGACCTGC ACTTTGGAGA GTTCCTCAAC 
ACCACCTGCA AGCACCTGAT GCGCCACTTC CCCGACCTGC TGGGCCGCTT GCTGAGCACC 
AGTCTGTTCT ACTATAAGAG CAGCTGGGAG GATGTCCGTG CTGCTGCCCC CATGCTCACA 
GGGTTCTTGG TGCTGCACAT GGAGGCTGAG CAGCGGCCAC AGGTGGACCT GGAGCAGCTC 
CTCACGGCAC TCCAGCTGCT GCTCAAGGAC CCAGCCCTTA AGGTGCGCTT GAAGGCTGTG 
AAGACTCTGG GCCGCTTGGT GAAGTTCGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MROH1 ( XM_010811847.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010810149.1 Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.

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CloneID	OBa49186
Clone ID Related Accession (Same CDS sequence)	XM_010811847.2
Accession Version	XM_010811847.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	maestro heat-like repeat-containing protein family member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811847.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010810149.1
CDS	163..4371
Translation	MSETYAKRLSAILLDAVTDKDPQMQEQVCGALCDFGESKPAEVLSACEEHLRLHEKLAHPYRTIILRAMEIVVSNHISELDKDTARAIILLASNEMTKVKELVSDWQQAASSVLVAVGRRFISSVMEELLSKFQPGLLPHPCTVHTLASLSVSNVFGVVPFLTSILSTMLPMLGAAKHDSMKVVFCCALQRFSESILEYLANLDQAPDPTVRKDAFASDIFGAYDILFHQWLQSGGAKLRLAVVEALGPMSHLLPSEKLEEQLPKLLPRVLAFYKKHTETVHVSKSLGQILEAAVSVGSRTLDIHLNSLLAALHAQICVPVESSSPLVMSNQKEVLRCFTVLACCSPDRLLAFLLPKLDTSNERTRVGTLQVLRHVINAAAAQMEVKKPVILSSMKLPLLDTNNKVKRAVVQVVSAMAHHGYLEQPGGKAMVEYIVQQCALPPEAEIQKLGADSEALAADSVRAISVSTLYLVSTTVDRMGEVLWPYLLEFLVPIRFTRALSPLCRSLVHLAQKRQEAGAHAPLIQYNGNVHLPSPYAITTRLLVEHCGPGRWWRPQLCLLQAVSSHPYVGDGRGAASLRLLNVLHQDIHPALGQRWVTAIPLLLEHLDEYSEETLSQKEWEEKLLVVREEEKTRVSLLWEQEEKTRFLRDSLAVVSDNTWVCHLTLEMCKQLPNYNGTPLEKNFLYKCVGTTLGAASSKVVRKHLRELLETARYQEEREHEGLACCFGICAISHLDDTLAQLDDFVKSDVLRKSAGIFNLFKNRSESEANKVRSALILCYGHVAAGAPRELLLARVEADLFWNMSECFSTKVLGIKVETQDPALRLSLVQSVCMATQAICSSAHSSSFHLSRKAELVAQMVEFIRAEPPDSLKTPIWKKAMLACTYLVTLEPALEEQVQADLIHSCLHRVMAVLPEPEEGDSPQEVSAAPTVGGDPQELLLFQSLYLDTVQVLKDLLTSLLLWNMTPLGLQVMLEHLSPWIKSPRGHERVRAVGLSACLLQFFLQHLQISALVPFHNLGLLIGLFSPRCADLWPATRREAVSCIHSLLYLQLTCVGFSRDYQDDVAEQLLSLKDGLVHPDPAILFHTCHSIAQLIAKRLPPDQLINLLLTLFESLGDPDKNCSRAASVMINCLLKERGNMLQEKVPEIVSVLRSKLQETQEEHILQAAQHSVFALATHHCASVVSNLLGSPLPFDSHTCTLWRALALEPGLAAQVLGLLLEKISKDVLFEESQAFLLSSTPDRVATLLPLAATCALHEVASAPASGPAVLELYPQLFVALLLRVSCTVGVQPPRQLQAKERRSASSGLASRSLEPCSSAVDALQAVLVRGGNEDVVQCMELDGGWQLLRTSAGHEEGVTRLASAMAKFAGPRLPLVMKLLFTTQSSMYEVQRVTSTAFLAE

Target ORF information:

RefSeq Version	XM_010811847.2
Organism	Bos taurus(cattle)
Definition	Bos taurus maestro heat like repeat family member 1 (MROH1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010811847.2

ORF Insert Sequence:

ATGAGCGAGA CCTACGCGAA GAGGCTGTCC GCCATCCTGC TGGACGCTGT CACTGACAAG 
GACCCTCAGA TGCAGGAGCA GGTATGTGGC GCCCTGTGCG ACTTCGGAGA GTCGAAGCCA 
GCGGAGGTTC TTAGCGCCTG CGAGGAGCAC CTGCGGCTGC ACGAAAAGCT AGCTCATCCA 
TACCGGACGA TAATCCTAAG GGCCATGGAG ATAGTCGTGA GCAATCACAT CAGCGAGCTG 
GACAAGGACA CGGCCAGGGC CATCATCCTC CTGGCCTCCA ATGAGATGAC CAAAGTGAAG 
GAGCTGGTCT CTGATTGGCA GCAAGCCGCC AGCAGCGTCC TGGTGGCGGT GGGAAGGCGG 
TTCATCAGCA GCGTGATGGA GGAGCTGCTG AGCAAGTTCC AGCCTGGGCT TCTGCCACAC 
CCCTGCACCG TGCACACGCT CGCCAGCCTC TCTGTCTCCA ATGTGTTTGG CGTGGTGCCC 
TTCCTGACGT CCATCCTCAG CACCATGCTG CCCATGCTGG GCGCAGCCAA GCACGACTCA 
ATGAAAGTAG TGTTCTGCTG TGCCCTGCAG CGCTTCAGTG AGAGTATCCT AGAATACTTG 
GCCAACCTGG ATCAGGCCCC AGACCCCACA GTCAGAAAGG ACGCCTTTGC CTCAGACATC 
TTTGGTGCTT ACGACATCCT TTTCCACCAG TGGCTACAGA GTGGGGGAGC GAAGCTGCGG 
CTTGCAGTGG TGGAGGCCCT GGGGCCCATG AGCCACCTGC TCCCCAGCGA GAAGCTGGAG 
GAGCAGCTCC CCAAGCTGCT GCCCAGGGTT CTTGCCTTCT ACAAGAAGCA CACCGAGACC 
GTCCATGTGT CCAAGAGCCT TGGCCAGATC CTGGAGGCAG CCGTGAGTGT GGGCAGCCGC 
ACTCTGGACA TCCACCTCAA CTCTCTCCTT GCCGCTCTGC ATGCTCAGAT CTGTGTGCCC 
GTGGAGTCGT CCAGCCCACT GGTGATGAGC AACCAGAAGG AGGTGCTGCG CTGCTTCACC 
GTGCTGGCCT GCTGCTCGCC GGACCGCCTG CTGGCCTTCC TGCTGCCCAA GCTGGACACC 
AGCAATGAGA GGACCCGTGT GGGCACCCTG CAGGTTCTGA GGCACGTCAT CAACGCGGCG 
GCTGCTCAGA TGGAAGTTAA GAAACCCGTC ATTCTCTCTT CCATGAAGCT CCCTCTTCTG 
GACACCAACA ACAAGGTGAA GCGGGCGGTG GTGCAGGTGG TCAGTGCCAT GGCCCACCAC 
GGCTACCTGG AGCAGCCTGG CGGCAAGGCC ATGGTCGAGT ACATCGTGCA GCAGTGCGCT 
CTGCCCCCCG AGGCTGAGAT TCAGAAGCTG GGTGCCGACA GTGAGGCCCT GGCGGCCGAT 
AGCGTGAGGG CCATCAGCGT CAGCACTCTC TACCTGGTCA GCACCACTGT GGACAGGATG 
GGTGAGGTCC TCTGGCCGTA CCTGCTTGAG TTCCTGGTCC CCATTCGCTT CACCAGGGCG 
CTGAGCCCAC TCTGCAGGAG CCTTGTGCAC TTGGCCCAGA AGAGGCAGGA GGCGGGGGCC 
CATGCTCCCC TCATCCAGTA CAACGGCAAC GTGCACCTCC CGTCTCCCTA CGCCATCACC 
ACCAGACTCC TGGTGGAGCA CTGTGGCCCA GGACGCTGGT GGAGGCCCCA ACTCTGCCTT 
CTGCAGGCCG TGTCTTCCCA TCCCTATGTG GGGGACGGTC GCGGGGCAGC CTCGCTGCGC 
CTCTTGAATG TCTTGCATCA GGACATCCAC CCGGCCCTGG GTCAGCGGTG GGTGACCGCC 
ATCCCCCTGC TGCTGGAGCA CCTGGACGAA TACAGTGAAG AAACGCTGTC ACAGAAGGAG 
TGGGAAGAAA AGCTGCTGGT GGTGAGAGAA GAGGAGAAGA CGAGAGTTAG CCTGCTTTGG 
GAACAGGAGG AGAAGACGAG ATTTCTCCGG GATTCCCTGG CTGTCGTGTC TGACAACACC 
TGGGTCTGCC ACCTGACCCT GGAAATGTGC AAGCAGCTAC CCAACTACAA CGGGACGCCC 
CTGGAGAAGA ACTTCCTGTA CAAATGTGTC GGGACCACCC TGGGTGCCGC TTCAAGTAAG 
GTGGTGAGGA AGCACCTGCG GGAGCTGCTG GAGACGGCCC GATACCAGGA GGAGAGGGAG 
CACGAGGGCC TTGCCTGTTG CTTTGGGATC TGTGCCATCT CCCACCTGGA TGACACCTTG 
GCCCAGCTGG ACGACTTTGT GAAGTCAGAC GTACTCAGAA AATCTGCTGG CATTTTCAAC 
CTTTTTAAGA ATCGAAGTGA GAGTGAGGCC AACAAAGTGA GGAGTGCGCT GATCCTGTGC 
TATGGGCATG TGGCGGCCGG GGCCCCGCGC GAGCTGCTGC TGGCCAGGGT GGAGGCGGAC 
TTGTTCTGGA ACATGTCCGA GTGCTTCAGC ACCAAGGTTC TGGGGATAAA GGTAGAAACC 
CAGGACCCGG CCCTGAGACT GAGCCTGGTG CAAAGTGTGT GCATGGCCAC CCAGGCCATC 
TGCAGCAGTG CCCACAGCAG CTCCTTCCAC CTCTCGAGGA AGGCGGAGCT GGTGGCGCAG 
ATGGTGGAGT TCATCAGAGC GGAGCCCCCA GACTCGCTGA AGACGCCCAT TTGGAAGAAG 
GCCATGCTTG CCTGCACGTA CCTGGTTACC CTGGAGCCGG CCCTGGAGGA GCAGGTGCAG 
GCGGACCTGA TTCACAGCTG CCTGCACCGT GTCATGGCCG TGCTGCCGGA GCCAGAGGAG 
GGGGACAGCC CCCAGGAGGT ATCAGCGGCC CCCACAGTGG GGGGGGACCC CCAGGAGCTT 
CTCTTGTTCC AGTCCCTGTA CCTGGACACC GTGCAGGTCC TCAAGGACTT GCTGACGAGC 
CTTCTTCTGT GGAACATGAC GCCCCTGGGT CTGCAGGTCA TGTTGGAGCA CCTGAGCCCA 
TGGATCAAGT CCCCGAGGGG CCACGAGCGG GTGCGGGCGG TCGGCCTGAG TGCCTGCCTG 
CTGCAGTTCT TCCTTCAACA CCTGCAGATC AGCGCCCTGG TGCCCTTCCA CAACCTGGGC 
CTCCTCATTG GCCTCTTCTC CCCACGGTGC GCCGACCTCT GGCCTGCCAC TCGCCGAGAG 
GCTGTGAGCT GCATCCACTC CCTGCTATAC CTGCAGCTGA CCTGTGTGGG CTTCTCGCGA 
GACTACCAGG ACGACGTGGC TGAGCAGCTC CTCAGCCTCA AAGATGGCCT GGTGCACCCT 
GACCCTGCCA TTCTCTTCCA TACCTGCCAC AGCATTGCCC AGCTTATTGC CAAGCGCCTC 
CCTCCAGATC AGCTCATCAA CCTCTTGCTA ACCTTGTTTG AGAGCCTGGG AGATCCCGAC 
AAGAACTGCT CGAGAGCTGC CAGCGTCATG ATCAACTGCC TGCTGAAGGA ACGGGGCAAC 
ATGCTGCAGG AGAAGGTGCC CGAGATTGTG AGTGTGCTGC GCTCCAAGCT GCAGGAGACC 
CAAGAGGAGC ACATCCTACA GGCCGCACAG CACAGCGTGT TTGCCCTGGC CACCCACCAC 
TGTGCCTCTG TGGTGTCCAA CCTTCTGGGC AGCCCCCTGC CCTTTGACAG CCACACCTGC 
ACCCTGTGGC GAGCACTGGC CTTGGAGCCC GGCCTCGCTG CACAGGTCCT GGGGCTGCTC 
CTGGAGAAGA TAAGCAAGGA CGTCCTGTTT GAGGAGAGCC AGGCCTTCCT GCTGAGCAGC 
ACGCCGGACC GCGTGGCCAC CCTGCTGCCC CTCGCAGCCA CCTGTGCACT GCACGAGGTC 
GCATCTGCCC CGGCGTCCGG GCCAGCAGTG CTGGAGCTCT ACCCCCAGCT GTTTGTGGCA 
CTGCTGCTGC GGGTCAGCTG CACCGTGGGT GTCCAGCCGC CCAGGCAGCT GCAGGCCAAG 
GAGAGGAGGA GCGCCAGCTC GGGCCTGGCC TCACGGAGCC TCGAGCCTTG CAGCTCTGCG 
GTGGACGCGC TGCAGGCCGT GCTTGTCCGC GGTGGCAATG AGGATGTGGT CCAGTGCATG 
GAGCTGGACG GGGGCTGGCA GTTGCTCAGG ACCTCGGCTG GGCACGAGGA AGGTGTCACC 
CGGCTGGCCA GTGCCATGGC AAAGTTCGCA GGCCCCCGGC TGCCCCTGGT GATGAAGCTG 
CTCTTCACCA CACAGAGTAG CATGTATGAG GTCCAGAGGG TCACCTCCAC AGCCTTCCTG 
GCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Assessment of the gene content of the chromosomal regions flanking bovine DGAT1.
Genomics83(1)172-80(2004 Jan)
Winter A,Alzinger A,Fries R

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MROH1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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