EMILIN2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMILIN2
Entrez Gene ID	514291
Full Name	elastin microfibril interfacer 2
General protein information	Preferred Names elastin microfibril interfacer 2 Names EMILIN-2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005224159.3	XP_005224216.1	EMILIN-2 isoform X1
XM_005224160.3	XP_005224217.1	EMILIN-2 isoform X2
XM_005224160.4	XP_005224217.2	EMILIN-2 isoform X2
XM_005224159.4	XP_005224216.2	EMILIN-2 isoform X1
NM_001143869.1	NP_001137341.1	EMILIN-2 precursor
XM_010818828.2	XP_010817130.1	EMILIN-2 isoform X3
NM_001143869.3	NP_001137341.2	EMILIN-2 precursor
NM_001143869.2	NP_001137341.1	EMILIN-2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EMILIN2	XP_003315909.1
Mus musculus (house mouse)	Emilin2	NP_660140.1
Gallus gallus (chicken)	EMILIN2	XP_419145.4
Danio rerio (zebrafish)	LOC100001725	XP_001341669.4
Canis lupus familiaris (dog)	EMILIN2	XP_005623089.1
Xenopus tropicalis (tropical clawed frog)	emilin2	XP_002938956.2
	EMILIN2	XP_001086149.1
Bos taurus (cattle)	EMILIN2	NP_001137341.1
Homo sapiens (human)	EMILIN2	NP_114437.2
Rattus norvegicus (Norway rat)	Emilin2	XP_006245732.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EMILIN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMILIN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa48904	XM_010818828.2 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa238985	NM_001143869.3 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa229878	XM_005224159.4 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa229879	XM_005224160.4 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa216402	NM_001143869.2 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa39599	NM_001143869.1 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa32530	XM_005224159.3 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00
OBa32531	XM_005224160.3 Latest version!	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$629.30 $899.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMILIN2 ( XM_010818828.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010817130.1 Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa48904
Clone ID Related Accession (Same CDS sequence)	XM_010818828.2
Accession Version	XM_010818828.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EMILIN-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCGCC CCATGCCTGC TCGGCCCCGC AACAGCATGA AGAAACCCAC AGACAATGAA 
CCAAGCCGAG TCTCAGAGCC TAAGAACACT GTGTCACCCA CTCCTGCAGC AGACCCAAGT 
CAGACAAGAG ATCCAAAACA GGGGCCCCAG GAACTTCAGG AAAAGAAAAT TCAGGTGCTG 
GAGGAGAAGG TTCTCCGGCT CACCAGGACG GTTCTGGACC TTCAGTCTTC CGTTGCTGGA 
GTGAGTGAAA ACCTCAAACA CGCCGTCCAG GACGATGCCA GCAAGATGCT GGCCTCATGG 
CTGAGCAACC TGCACCCCCG GCCAACGCCC ATCAGCGCTG TGGGTGGAGA ACCAGAAACA 
GTTCAGCCCC CCGGCATCCT CAGCAATAAG GAGTCAGGCA TGAAGGACAT CAAGTCTGAA 
CTGGCTGAAG TCAAGGATGC CCTGAAGACC AAGAGTGACA AGCTGGAGGA GCTGGACGGG 
AAAGTGAAGG GCTATGAAGG GCAGCTCAGA CAGCTCCAGG AGGCCGCCCA GGGCCCAACG 
GTCACCATGA CGACCAGCGA GCTCTTCCAA GCCTACGTGG ACAGTAAGAT CGATGCCCTG 
AGGGAGGAGC TTATGGAAGG GATGGACCGA AAGCTGGCTG ACCTGAAGAA CTCGTGTGAG 
TACAAGTTGA TCGGTTTCCA GCAGCACTGT GATGACTATG GGAGCAGCTA CCTGGGAGTG 
ATCGAGCTGA TAGGAGAGAA GGAGGCCAAG CTGAGAAAAG AAATCAGTGA CCTTCAGGCC 
CGGGTCCAAG ACCCTCCAGC CCATGCAAAC TGCTGTGACG GTACCAAGCC TGGTGACTTG 
GGGCCACAGA TCAAGATGTT AGACCAGAAA ATCGAAAGAG TTTCTGAAGC GACAAGAATG 
CTGAATGCAC GACTAGACAA TGAGTTTGAC CGCCTCGAGG CTCCAGAGCC GGATGTGGAC 
TTCGATGCCA GATGGCATGA ACTGGATGCA AGGATCAACG TGACAGAGAG GAATGCAGAA 
GAGCACTGCT TTTACATTGA AGAAACCCTC CGGGGGACCA TTAGTGGGGA GGTGGACGGC 
CTGAGGCAGC TGCTTGATCA GAAAATACAG TCCCTGGAGG AGCGGCTGGG GAGCGCCCTC 
CTGGAGATGG TGAACGGCTC AGAGATGGTA CCTGCTCCCC CAGTGGCAGC CCCGCCCACT 
GTGTCGGGGG CTGGGCCCCC GCAGGTCATG ATGGAGTTGA AGCGCCTGAA GGACCAAGTG 
CGGGTGGTGG AGGACATGTG CCTGCAGAAC TTCCGAGGAG AGCCTCGAGG GATGGAAGAC 
ACTTTGCGGA ACGGGGGCGA GATGGTGAGC CTTTTGAGAT CTCTAAACGA CACGATGCAG 
GGGAAGTTTC AGGACACTGA ACGCAGCATC CAGCGACTTC AGCAGGACTT CAGTTTTCTT 
TATTCTTGGC TCAACCACAC AGATGACCAC TTGAGACGTC TACAGAACGA GCTGAGTGGT 
GGTAGAGGAG GGAAAAAGGC TGCAGGGGGT GGGTGGTCCA AGGTGGGTGA GCCAGGGAGG 
ACGGAGGCGC CCCTGCCGTC TCCCCAGGCC CCCCCAGCGC ATTGCTGCGA CCAGCTGGAG 
GGCAGGTGGC AGCGGCTGCA GGACCTCGTC CTCAGCGAGC TGGACACTTG TAAGGAGAAG 
ACGCACGGAG TCCAGAGGGA GGTGTCTGTG GTAGAGGACA GGGTGTCTCA CGTGGAGAAG 
ACCTGTAGCA AGCTGGACTC CATCTCTGGG AGCCTTCAGA AGATCAAGGA GGGGCTCAAC 
AAGCACGTGA ACAGCCTCTG GAGCTGCATC CGGCAGATGA ACGGGACGCT CCGGACACAC 
TCCAGAGAGA TCTCCAGCCT GAAGAATTCC GTCCAGCAGT TCTACAGCCA CGTCTTCCAG 
ATTTCTGCTG ACCTGCAAGA CCTGATCCGG TTTCAGCCAT CGGCAACAGA GGACCCCTCA 
GAAACGCCTC AGCCTCCACC CCGGGGGAAG ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC 
CCGGCAGAGG CCCCCACGGA GCTGTCCACT CCAGTGCTGA CCCCACTGCT GCCGCCCAGC 
ACTCCCTCGC CTCCGAGGGA CCCAGGACAG AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA 
CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC TTCTTGCCTG GCTCCACGGG GGTCATCATG 
GAGACTGGAG AGGCCGGCCC CCCAGGCAGG ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG 
GGTGTGGATG GCCAGACCGG GCAGCGGCCA ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT 
CCAGGGTACC CCAAATCACC GCCTGCAGCC TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG 
TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC 
AACAAGGTGC TGGTGAACGA CGGGGACGTG TACGACCCCA GCACCGGGGT CTTCACGGCT 
CCGTACGACG GGCGCTACCT GATCACGGCC ACCCTCACAC CCGAGAGGGA TGCGTATGTG 
GAGGCCGTCC TGTCAGTGGC CAACGCCAGC GTGGCCCAGC TGCACACGGC CGGCTACCGG 
AGGGAGTTCC TGGAGTACCA CCGGCCACAG GGGGCCCCAC ACACCTGCGG GGGCCCGGGC 
GCCTTCCACC TCATCGTGCA CCTCAAGGCG GGGGACGGCG TCAACATCGT GGTCACGGGG 
GGCCGGCTGG CCCACACGGA CTTTGACGAG ATGTACTCCA CCTTCAGCGG GGTCTTCTTG 
TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010817130.1
CDS	298..3141
Translation	MPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISSLKNSVQQFYSHVFQISADLQDLIRFQPSATEDPSETPQPPPRGKTPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSVGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_010818828.2
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010818828.2

ORF Insert Sequence:

ATGCCCCGCC CCATGCCTGC TCGGCCCCGC AACAGCATGA AGAAACCCAC AGACAATGAA 
CCAAGCCGAG TCTCAGAGCC TAAGAACACT GTGTCACCCA CTCCTGCAGC AGACCCAAGT 
CAGACAAGAG ATCCAAAACA GGGGCCCCAG GAACTTCAGG AAAAGAAAAT TCAGGTGCTG 
GAGGAGAAGG TTCTCCGGCT CACCAGGACG GTTCTGGACC TTCAGTCTTC CGTTGCTGGA 
GTGAGTGAAA ACCTCAAACA CGCCGTCCAG GACGATGCCA GCAAGATGCT GGCCTCATGG 
CTGAGCAACC TGCACCCCCG GCCAACGCCC ATCAGCGCTG TGGGTGGAGA ACCAGAAACA 
GTTCAGCCCC CCGGCATCCT CAGCAATAAG GAGTCAGGCA TGAAGGACAT CAAGTCTGAA 
CTGGCTGAAG TCAAGGATGC CCTGAAGACC AAGAGTGACA AGCTGGAGGA GCTGGACGGG 
AAAGTGAAGG GCTATGAAGG GCAGCTCAGA CAGCTCCAGG AGGCCGCCCA GGGCCCAACG 
GTCACCATGA CGACCAGCGA GCTCTTCCAA GCCTACGTGG ACAGTAAGAT CGATGCCCTG 
AGGGAGGAGC TTATGGAAGG GATGGACCGA AAGCTGGCTG ACCTGAAGAA CTCGTGTGAG 
TACAAGTTGA TCGGTTTCCA GCAGCACTGT GATGACTATG GGAGCAGCTA CCTGGGAGTG 
ATCGAGCTGA TAGGAGAGAA GGAGGCCAAG CTGAGAAAAG AAATCAGTGA CCTTCAGGCC 
CGGGTCCAAG ACCCTCCAGC CCATGCAAAC TGCTGTGACG GTACCAAGCC TGGTGACTTG 
GGGCCACAGA TCAAGATGTT AGACCAGAAA ATCGAAAGAG TTTCTGAAGC GACAAGAATG 
CTGAATGCAC GACTAGACAA TGAGTTTGAC CGCCTCGAGG CTCCAGAGCC GGATGTGGAC 
TTCGATGCCA GATGGCATGA ACTGGATGCA AGGATCAACG TGACAGAGAG GAATGCAGAA 
GAGCACTGCT TTTACATTGA AGAAACCCTC CGGGGGACCA TTAGTGGGGA GGTGGACGGC 
CTGAGGCAGC TGCTTGATCA GAAAATACAG TCCCTGGAGG AGCGGCTGGG GAGCGCCCTC 
CTGGAGATGG TGAACGGCTC AGAGATGGTA CCTGCTCCCC CAGTGGCAGC CCCGCCCACT 
GTGTCGGGGG CTGGGCCCCC GCAGGTCATG ATGGAGTTGA AGCGCCTGAA GGACCAAGTG 
CGGGTGGTGG AGGACATGTG CCTGCAGAAC TTCCGAGGAG AGCCTCGAGG GATGGAAGAC 
ACTTTGCGGA ACGGGGGCGA GATGGTGAGC CTTTTGAGAT CTCTAAACGA CACGATGCAG 
GGGAAGTTTC AGGACACTGA ACGCAGCATC CAGCGACTTC AGCAGGACTT CAGTTTTCTT 
TATTCTTGGC TCAACCACAC AGATGACCAC TTGAGACGTC TACAGAACGA GCTGAGTGGT 
GGTAGAGGAG GGAAAAAGGC TGCAGGGGGT GGGTGGTCCA AGGTGGGTGA GCCAGGGAGG 
ACGGAGGCGC CCCTGCCGTC TCCCCAGGCC CCCCCAGCGC ATTGCTGCGA CCAGCTGGAG 
GGCAGGTGGC AGCGGCTGCA GGACCTCGTC CTCAGCGAGC TGGACACTTG TAAGGAGAAG 
ACGCACGGAG TCCAGAGGGA GGTGTCTGTG GTAGAGGACA GGGTGTCTCA CGTGGAGAAG 
ACCTGTAGCA AGCTGGACTC CATCTCTGGG AGCCTTCAGA AGATCAAGGA GGGGCTCAAC 
AAGCACGTGA ACAGCCTCTG GAGCTGCATC CGGCAGATGA ACGGGACGCT CCGGACACAC 
TCCAGAGAGA TCTCCAGCCT GAAGAATTCC GTCCAGCAGT TCTACAGCCA CGTCTTCCAG 
ATTTCTGCTG ACCTGCAAGA CCTGATCCGG TTTCAGCCAT CGGCAACAGA GGACCCCTCA 
GAAACGCCTC AGCCTCCACC CCGGGGGAAG ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC 
CCGGCAGAGG CCCCCACGGA GCTGTCCACT CCAGTGCTGA CCCCACTGCT GCCGCCCAGC 
ACTCCCTCGC CTCCGAGGGA CCCAGGACAG AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA 
CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC TTCTTGCCTG GCTCCACGGG GGTCATCATG 
GAGACTGGAG AGGCCGGCCC CCCAGGCAGG ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG 
GGTGTGGATG GCCAGACCGG GCAGCGGCCA ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT 
CCAGGGTACC CCAAATCACC GCCTGCAGCC TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG 
TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC 
AACAAGGTGC TGGTGAACGA CGGGGACGTG TACGACCCCA GCACCGGGGT CTTCACGGCT 
CCGTACGACG GGCGCTACCT GATCACGGCC ACCCTCACAC CCGAGAGGGA TGCGTATGTG 
GAGGCCGTCC TGTCAGTGGC CAACGCCAGC GTGGCCCAGC TGCACACGGC CGGCTACCGG 
AGGGAGTTCC TGGAGTACCA CCGGCCACAG GGGGCCCCAC ACACCTGCGG GGGCCCGGGC 
GCCTTCCACC TCATCGTGCA CCTCAAGGCG GGGGACGGCG TCAACATCGT GGTCACGGGG 
GGCCGGCTGG CCCACACGGA CTTTGACGAG ATGTACTCCA CCTTCAGCGG GGTCTTCTTG 
TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( NM_001143869.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137341.2 Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

Price & Availability↑

CloneID	OBa238985
Clone ID Related Accession (Same CDS sequence)	NM_001143869.3
Accession Version	NM_001143869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	EMILIN-2 precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000024.1. On May 29, 2018 this sequence version replaced NM_001143869.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGGC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCCGCG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137341.2
CDS	1..3222
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISGLKNSVQQFYSHVFQISADLQDLIRFQPSAKTPQPPPRGKAPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSAGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001143869.3
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143869.3

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGGC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCCGCG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_005224159.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224216.2 Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa229878
Clone ID Related Accession (Same CDS sequence)	XM_005224159.4
Accession Version	XM_005224159.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EMILIN-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224159.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG 
AAGGCCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC 
ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA 
CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG 
CCCTTCTTGC CTGGCTCCAC AGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC 
AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG 
CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA 
GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGCGGGGCT CACCCGGAAG 
CCCTTCCCCA GCGACGCCGG CGTAGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC 
GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG 
GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC 
AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA 
CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG 
GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC 
GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224216.2
CDS	151..3387
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISGLKNSVQQFYSHVFQISADLQDLIRFQPSATEDPSETPQPPPRGKAPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSAGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_005224159.4
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224159.4

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG 
AAGGCCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC 
ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA 
CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG 
CCCTTCTTGC CTGGCTCCAC AGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC 
AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG 
CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA 
GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGCGGGGCT CACCCGGAAG 
CCCTTCCCCA GCGACGCCGG CGTAGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC 
GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG 
GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC 
AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA 
CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG 
GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC 
GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_005224160.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224217.2 Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229879
Clone ID Related Accession (Same CDS sequence)	XM_005224160.4
Accession Version	XM_005224160.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	EMILIN-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224160.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG 
GCCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT 
CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG 
AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC 
TTCTTGCCTG GCTCCACAGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG 
ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA 
ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC 
TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG CGGGGCTCAC CCGGAAGCCC 
TTCCCCAGCG ACGCCGGCGT AGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG 
TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC 
ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC 
GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG 
GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG 
GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG 
ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224217.2
CDS	151..3384
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISGLKNSVQQFYSHVFQISADLQDLIRFQPSAKDPSETPQPPPRGKAPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSAGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_005224160.4
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224160.4

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG 
GCCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT 
CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG 
AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC 
TTCTTGCCTG GCTCCACAGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG 
ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA 
ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC 
TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG CGGGGCTCAC CCGGAAGCCC 
TTCCCCAGCG ACGCCGGCGT AGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG 
TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC 
ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC 
GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG 
GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG 
GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG 
ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( NM_001143869.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137341.1 Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

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CloneID	OBa216402
Clone ID Related Accession (Same CDS sequence)	NM_001143869.2
Accession Version	NM_001143869.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	EMILIN-2 precursor
Comment	Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02056716.1, DAAA02056717.1 and DAAA02056719.1. On Jun 30, 2017 this sequence version replaced NM_001143869.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403657, SAMN02822088 [ECO:0000350] ##Evidence-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACGGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCCGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTGG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137341.1
CDS	1..3222
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISSLKNSVQQFYSHVFQISADLQDLIRFQPSAKTPQPPPRGKTPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSVGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001143869.2
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143869.2

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACGGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCCGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTGG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( NM_001143869.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137341.1 Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

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CloneID	OBa39599
Clone ID Related Accession (Same CDS sequence)	NM_001143869.1
Accession Version	NM_001143869.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	EMILIN-2 precursor
Comment	INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03019644.1, AAFC03064819.1 and AAFC03064821.1. On Jan 8, 2009 this sequence version replaced XM_592120.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN01093727 [ECO:0000350] ##Evidence-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGTGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCTGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCGCAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGTGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137341.1
CDS	1..3222
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISSLKNSVQQFYSHVFQISADLQDLIRFQPSAKTPQPPPRGKTPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSVGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	NM_001143869.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143869.1

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC 
GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC 
CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG 
CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC 
TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA 
GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGTGCC 
GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT 
CCGGTGCCAT CTCTCGTGTC CTTCTCTGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC 
GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC 
ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC 
GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG 
CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCGCAGGG GGCCCCACAC 
ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGTGTC 
AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC 
TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_005224159.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224216.1 Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa32530
Clone ID Related Accession (Same CDS sequence)	XM_005224159.3
Accession Version	XM_005224159.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EMILIN-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG 
AAGACCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC 
ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA 
CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG 
CCCTTCTTGC CTGGCTCCAC GGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC 
AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG 
CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA 
GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGTGGGGCT CACCCGGAAG 
CCCTTCCCCA GCGACGCCGG CGTGGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC 
GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG 
GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC 
AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA 
CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG 
GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC 
GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224216.1
CDS	421..3657
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISSLKNSVQQFYSHVFQISADLQDLIRFQPSATEDPSETPQPPPRGKTPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSVGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_005224159.3
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224159.3

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG 
AAGACCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC 
ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA 
CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG 
CCCTTCTTGC CTGGCTCCAC GGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC 
AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG 
CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA 
GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGTGGGGCT CACCCGGAAG 
CCCTTCCCCA GCGACGCCGG CGTGGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC 
GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG 
GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC 
AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA 
CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG 
GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC 
GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMILIN2 ( XM_005224160.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005224217.1 Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

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CloneID	OBa32531
Clone ID Related Accession (Same CDS sequence)	XM_005224160.3
Accession Version	XM_005224160.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	EMILIN-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG 
ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT 
CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG 
AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC 
TTCTTGCCTG GCTCCACGGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG 
ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA 
ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC 
TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC 
TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG 
TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC 
ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC 
GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG 
GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG 
GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG 
ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005224217.1
CDS	1..3234
Translation	MWPPTWPRPRPRPRAPWRWALALLALGGAGLCHASPQPGHPARPSARNKNWCAYIVNKNVSCSVLEGSETFIQAQYNCGWNQKPCPSALMYRVNVRPRYVTRYKTVTQLEWRCCPGFRGGNCQEGPRDPGRMPRPMPARPRNSMKKPTDNEPSRVSEPKNTVSPTPAADPSQTRDPKQGPQELQEKKIQVLEEKVLRLTRTVLDLQSSVAGVSENLKHAVQDDASKMLASWLSNLHPRPTPISAVGGEPETVQPPGILSNKESGMKDIKSELAEVKDALKTKSDKLEELDGKVKGYEGQLRQLQEAAQGPTVTMTTSELFQAYVDSKIDALREELMEGMDRKLADLKNSCEYKLIGFQQHCDDYGSSYLGVIELIGEKEAKLRKEISDLQARVQDPPAHANCCDGTKPGDLGPQIKMLDQKIERVSEATRMLNARLDNEFDRLEAPEPDVDFDARWHELDARINVTERNAEEHCFYIEETLRGTISGEVDGLRQLLDQKIQSLEERLGSALLEMVNGSEMVPAPPVAAPPTVSGAGPPQVMMELKRLKDQVRVVEDMCLQNFRGEPRGMEDTLRNGGEMVSLLRSLNDTMQGKFQDTERSIQRLQQDFSFLYSWLNHTDDHLRRLQNELSGGRGGKKAAGGGWSKVGEPGRTEAPLPSPQAPPAHCCDQLEGRWQRLQDLVLSELDTCKEKTHGVQREVSVVEDRVSHVEKTCSKLDSISGSLQKIKEGLNKHVNSLWSCIRQMNGTLRTHSREISSLKNSVQQFYSHVFQISADLQDLIRFQPSAKDPSETPQPPPRGKTPEDVGKPSSPAEAPTELSTPVLTPLLPPSTPSPPRDPGQKPVLPKRRPPPLRPGWTGLPFLPGSTGVIMETGEAGPPGRMGVSGRGLPRGVDGQTGQRPIPSAEGYAGAPGYPKSPPAASPGAPVPSLVSFSVGLTRKPFPSDAGVVLFNKVLVNDGDVYDPSTGVFTAPYDGRYLITATLTPERDAYVEAVLSVANASVAQLHTAGYRREFLEYHRPQGAPHTCGGPGAFHLIVHLKAGDGVNIVVTGGRLAHTDFDEMYSTFSGVFLYPFLSHL

Target ORF information:

RefSeq Version	XM_005224160.3
Organism	Bos taurus(cattle)
Definition	Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005224160.3

ORF Insert Sequence:

ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC 
CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG 
AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG 
AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC 
ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA 
AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC 
CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC 
ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC 
CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG 
ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC 
CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG 
CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT 
AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG 
ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC 
AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC 
CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC 
CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC 
TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC 
AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA 
AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG 
AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT 
GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT 
GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC 
CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA 
CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG 
GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC 
ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG 
AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG 
AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC 
ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC 
CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG 
GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG 
GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC 
GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT 
GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT 
GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC 
ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT 
TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC 
CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG 
ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT 
CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG 
AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC 
TTCTTGCCTG GCTCCACGGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG 
ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA 
ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC 
TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC 
TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG 
TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC 
ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC 
GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG 
GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG 
GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG 
ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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EMILIN2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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