EMILIN2 cDNA ORF clone, Bos taurus(cattle)

The following EMILIN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMILIN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa48904 XM_010818828.2
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa238985 NM_001143869.3
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa229878 XM_005224159.4
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa229879 XM_005224160.4
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa216402 NM_001143869.2
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa39599 NM_001143869.1
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32530 XM_005224159.3
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OBa32531 XM_005224160.3
Latest version!
Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa48904
    Clone ID Related Accession (Same CDS sequence) XM_010818828.2
    Accession Version XM_010818828.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EMILIN-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818828.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCCGCC CCATGCCTGC TCGGCCCCGC AACAGCATGA AGAAACCCAC AGACAATGAA 
    CCAAGCCGAG TCTCAGAGCC TAAGAACACT GTGTCACCCA CTCCTGCAGC AGACCCAAGT
    CAGACAAGAG ATCCAAAACA GGGGCCCCAG GAACTTCAGG AAAAGAAAAT TCAGGTGCTG
    GAGGAGAAGG TTCTCCGGCT CACCAGGACG GTTCTGGACC TTCAGTCTTC CGTTGCTGGA
    GTGAGTGAAA ACCTCAAACA CGCCGTCCAG GACGATGCCA GCAAGATGCT GGCCTCATGG
    CTGAGCAACC TGCACCCCCG GCCAACGCCC ATCAGCGCTG TGGGTGGAGA ACCAGAAACA
    GTTCAGCCCC CCGGCATCCT CAGCAATAAG GAGTCAGGCA TGAAGGACAT CAAGTCTGAA
    CTGGCTGAAG TCAAGGATGC CCTGAAGACC AAGAGTGACA AGCTGGAGGA GCTGGACGGG
    AAAGTGAAGG GCTATGAAGG GCAGCTCAGA CAGCTCCAGG AGGCCGCCCA GGGCCCAACG
    GTCACCATGA CGACCAGCGA GCTCTTCCAA GCCTACGTGG ACAGTAAGAT CGATGCCCTG
    AGGGAGGAGC TTATGGAAGG GATGGACCGA AAGCTGGCTG ACCTGAAGAA CTCGTGTGAG
    TACAAGTTGA TCGGTTTCCA GCAGCACTGT GATGACTATG GGAGCAGCTA CCTGGGAGTG
    ATCGAGCTGA TAGGAGAGAA GGAGGCCAAG CTGAGAAAAG AAATCAGTGA CCTTCAGGCC
    CGGGTCCAAG ACCCTCCAGC CCATGCAAAC TGCTGTGACG GTACCAAGCC TGGTGACTTG
    GGGCCACAGA TCAAGATGTT AGACCAGAAA ATCGAAAGAG TTTCTGAAGC GACAAGAATG
    CTGAATGCAC GACTAGACAA TGAGTTTGAC CGCCTCGAGG CTCCAGAGCC GGATGTGGAC
    TTCGATGCCA GATGGCATGA ACTGGATGCA AGGATCAACG TGACAGAGAG GAATGCAGAA
    GAGCACTGCT TTTACATTGA AGAAACCCTC CGGGGGACCA TTAGTGGGGA GGTGGACGGC
    CTGAGGCAGC TGCTTGATCA GAAAATACAG TCCCTGGAGG AGCGGCTGGG GAGCGCCCTC
    CTGGAGATGG TGAACGGCTC AGAGATGGTA CCTGCTCCCC CAGTGGCAGC CCCGCCCACT
    GTGTCGGGGG CTGGGCCCCC GCAGGTCATG ATGGAGTTGA AGCGCCTGAA GGACCAAGTG
    CGGGTGGTGG AGGACATGTG CCTGCAGAAC TTCCGAGGAG AGCCTCGAGG GATGGAAGAC
    ACTTTGCGGA ACGGGGGCGA GATGGTGAGC CTTTTGAGAT CTCTAAACGA CACGATGCAG
    GGGAAGTTTC AGGACACTGA ACGCAGCATC CAGCGACTTC AGCAGGACTT CAGTTTTCTT
    TATTCTTGGC TCAACCACAC AGATGACCAC TTGAGACGTC TACAGAACGA GCTGAGTGGT
    GGTAGAGGAG GGAAAAAGGC TGCAGGGGGT GGGTGGTCCA AGGTGGGTGA GCCAGGGAGG
    ACGGAGGCGC CCCTGCCGTC TCCCCAGGCC CCCCCAGCGC ATTGCTGCGA CCAGCTGGAG
    GGCAGGTGGC AGCGGCTGCA GGACCTCGTC CTCAGCGAGC TGGACACTTG TAAGGAGAAG
    ACGCACGGAG TCCAGAGGGA GGTGTCTGTG GTAGAGGACA GGGTGTCTCA CGTGGAGAAG
    ACCTGTAGCA AGCTGGACTC CATCTCTGGG AGCCTTCAGA AGATCAAGGA GGGGCTCAAC
    AAGCACGTGA ACAGCCTCTG GAGCTGCATC CGGCAGATGA ACGGGACGCT CCGGACACAC
    TCCAGAGAGA TCTCCAGCCT GAAGAATTCC GTCCAGCAGT TCTACAGCCA CGTCTTCCAG
    ATTTCTGCTG ACCTGCAAGA CCTGATCCGG TTTCAGCCAT CGGCAACAGA GGACCCCTCA
    GAAACGCCTC AGCCTCCACC CCGGGGGAAG ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC
    CCGGCAGAGG CCCCCACGGA GCTGTCCACT CCAGTGCTGA CCCCACTGCT GCCGCCCAGC
    ACTCCCTCGC CTCCGAGGGA CCCAGGACAG AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA
    CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC TTCTTGCCTG GCTCCACGGG GGTCATCATG
    GAGACTGGAG AGGCCGGCCC CCCAGGCAGG ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG
    GGTGTGGATG GCCAGACCGG GCAGCGGCCA ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT
    CCAGGGTACC CCAAATCACC GCCTGCAGCC TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG
    TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC
    AACAAGGTGC TGGTGAACGA CGGGGACGTG TACGACCCCA GCACCGGGGT CTTCACGGCT
    CCGTACGACG GGCGCTACCT GATCACGGCC ACCCTCACAC CCGAGAGGGA TGCGTATGTG
    GAGGCCGTCC TGTCAGTGGC CAACGCCAGC GTGGCCCAGC TGCACACGGC CGGCTACCGG
    AGGGAGTTCC TGGAGTACCA CCGGCCACAG GGGGCCCCAC ACACCTGCGG GGGCCCGGGC
    GCCTTCCACC TCATCGTGCA CCTCAAGGCG GGGGACGGCG TCAACATCGT GGTCACGGGG
    GGCCGGCTGG CCCACACGGA CTTTGACGAG ATGTACTCCA CCTTCAGCGG GGTCTTCTTG
    TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817130.1
    CDS298..3141
    Translation

    Target ORF information:

    RefSeq Version XM_010818828.2
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818828.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGCCCCGCC CCATGCCTGC TCGGCCCCGC AACAGCATGA AGAAACCCAC AGACAATGAA 
    CCAAGCCGAG TCTCAGAGCC TAAGAACACT GTGTCACCCA CTCCTGCAGC AGACCCAAGT
    CAGACAAGAG ATCCAAAACA GGGGCCCCAG GAACTTCAGG AAAAGAAAAT TCAGGTGCTG
    GAGGAGAAGG TTCTCCGGCT CACCAGGACG GTTCTGGACC TTCAGTCTTC CGTTGCTGGA
    GTGAGTGAAA ACCTCAAACA CGCCGTCCAG GACGATGCCA GCAAGATGCT GGCCTCATGG
    CTGAGCAACC TGCACCCCCG GCCAACGCCC ATCAGCGCTG TGGGTGGAGA ACCAGAAACA
    GTTCAGCCCC CCGGCATCCT CAGCAATAAG GAGTCAGGCA TGAAGGACAT CAAGTCTGAA
    CTGGCTGAAG TCAAGGATGC CCTGAAGACC AAGAGTGACA AGCTGGAGGA GCTGGACGGG
    AAAGTGAAGG GCTATGAAGG GCAGCTCAGA CAGCTCCAGG AGGCCGCCCA GGGCCCAACG
    GTCACCATGA CGACCAGCGA GCTCTTCCAA GCCTACGTGG ACAGTAAGAT CGATGCCCTG
    AGGGAGGAGC TTATGGAAGG GATGGACCGA AAGCTGGCTG ACCTGAAGAA CTCGTGTGAG
    TACAAGTTGA TCGGTTTCCA GCAGCACTGT GATGACTATG GGAGCAGCTA CCTGGGAGTG
    ATCGAGCTGA TAGGAGAGAA GGAGGCCAAG CTGAGAAAAG AAATCAGTGA CCTTCAGGCC
    CGGGTCCAAG ACCCTCCAGC CCATGCAAAC TGCTGTGACG GTACCAAGCC TGGTGACTTG
    GGGCCACAGA TCAAGATGTT AGACCAGAAA ATCGAAAGAG TTTCTGAAGC GACAAGAATG
    CTGAATGCAC GACTAGACAA TGAGTTTGAC CGCCTCGAGG CTCCAGAGCC GGATGTGGAC
    TTCGATGCCA GATGGCATGA ACTGGATGCA AGGATCAACG TGACAGAGAG GAATGCAGAA
    GAGCACTGCT TTTACATTGA AGAAACCCTC CGGGGGACCA TTAGTGGGGA GGTGGACGGC
    CTGAGGCAGC TGCTTGATCA GAAAATACAG TCCCTGGAGG AGCGGCTGGG GAGCGCCCTC
    CTGGAGATGG TGAACGGCTC AGAGATGGTA CCTGCTCCCC CAGTGGCAGC CCCGCCCACT
    GTGTCGGGGG CTGGGCCCCC GCAGGTCATG ATGGAGTTGA AGCGCCTGAA GGACCAAGTG
    CGGGTGGTGG AGGACATGTG CCTGCAGAAC TTCCGAGGAG AGCCTCGAGG GATGGAAGAC
    ACTTTGCGGA ACGGGGGCGA GATGGTGAGC CTTTTGAGAT CTCTAAACGA CACGATGCAG
    GGGAAGTTTC AGGACACTGA ACGCAGCATC CAGCGACTTC AGCAGGACTT CAGTTTTCTT
    TATTCTTGGC TCAACCACAC AGATGACCAC TTGAGACGTC TACAGAACGA GCTGAGTGGT
    GGTAGAGGAG GGAAAAAGGC TGCAGGGGGT GGGTGGTCCA AGGTGGGTGA GCCAGGGAGG
    ACGGAGGCGC CCCTGCCGTC TCCCCAGGCC CCCCCAGCGC ATTGCTGCGA CCAGCTGGAG
    GGCAGGTGGC AGCGGCTGCA GGACCTCGTC CTCAGCGAGC TGGACACTTG TAAGGAGAAG
    ACGCACGGAG TCCAGAGGGA GGTGTCTGTG GTAGAGGACA GGGTGTCTCA CGTGGAGAAG
    ACCTGTAGCA AGCTGGACTC CATCTCTGGG AGCCTTCAGA AGATCAAGGA GGGGCTCAAC
    AAGCACGTGA ACAGCCTCTG GAGCTGCATC CGGCAGATGA ACGGGACGCT CCGGACACAC
    TCCAGAGAGA TCTCCAGCCT GAAGAATTCC GTCCAGCAGT TCTACAGCCA CGTCTTCCAG
    ATTTCTGCTG ACCTGCAAGA CCTGATCCGG TTTCAGCCAT CGGCAACAGA GGACCCCTCA
    GAAACGCCTC AGCCTCCACC CCGGGGGAAG ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC
    CCGGCAGAGG CCCCCACGGA GCTGTCCACT CCAGTGCTGA CCCCACTGCT GCCGCCCAGC
    ACTCCCTCGC CTCCGAGGGA CCCAGGACAG AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA
    CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC TTCTTGCCTG GCTCCACGGG GGTCATCATG
    GAGACTGGAG AGGCCGGCCC CCCAGGCAGG ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG
    GGTGTGGATG GCCAGACCGG GCAGCGGCCA ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT
    CCAGGGTACC CCAAATCACC GCCTGCAGCC TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG
    TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC
    AACAAGGTGC TGGTGAACGA CGGGGACGTG TACGACCCCA GCACCGGGGT CTTCACGGCT
    CCGTACGACG GGCGCTACCT GATCACGGCC ACCCTCACAC CCGAGAGGGA TGCGTATGTG
    GAGGCCGTCC TGTCAGTGGC CAACGCCAGC GTGGCCCAGC TGCACACGGC CGGCTACCGG
    AGGGAGTTCC TGGAGTACCA CCGGCCACAG GGGGCCCCAC ACACCTGCGG GGGCCCGGGC
    GCCTTCCACC TCATCGTGCA CCTCAAGGCG GGGGACGGCG TCAACATCGT GGTCACGGGG
    GGCCGGCTGG CCCACACGGA CTTTGACGAG ATGTACTCCA CCTTCAGCGG GGTCTTCTTG
    TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa238985
    Clone ID Related Accession (Same CDS sequence) NM_001143869.3
    Accession Version NM_001143869.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-28
    Organism Bos taurus(cattle)
    Product EMILIN-2 precursor
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from NKLS02000024.1. On May 29, 2018 this sequence version replaced NM_001143869.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGGC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCCGCG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137341.2
    CDS1..3222
    Translation

    Target ORF information:

    RefSeq Version NM_001143869.3
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143869.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGGC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCCGCG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229878
    Clone ID Related Accession (Same CDS sequence) XM_005224159.4
    Accession Version XM_005224159.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product EMILIN-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224159.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG
    AAGGCCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC
    ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA
    CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG
    CCCTTCTTGC CTGGCTCCAC AGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC
    AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG
    CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA
    GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGCGGGGCT CACCCGGAAG
    CCCTTCCCCA GCGACGCCGG CGTAGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC
    GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG
    GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC
    AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA
    CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG
    GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC
    GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224216.2
    CDS151..3387
    Translation

    Target ORF information:

    RefSeq Version XM_005224159.4
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224159.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG
    AAGGCCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC
    ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA
    CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG
    CCCTTCTTGC CTGGCTCCAC AGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC
    AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG
    CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA
    GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGCGGGGCT CACCCGGAAG
    CCCTTCCCCA GCGACGCCGG CGTAGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC
    GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG
    GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC
    AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA
    CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG
    GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC
    GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229879
    Clone ID Related Accession (Same CDS sequence) XM_005224160.4
    Accession Version XM_005224160.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product EMILIN-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037351.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224160.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG
    GCCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT
    CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG
    AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC
    TTCTTGCCTG GCTCCACAGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG
    ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA
    ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC
    TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG CGGGGCTCAC CCGGAAGCCC
    TTCCCCAGCG ACGCCGGCGT AGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG
    TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC
    ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC
    GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG
    GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG
    GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG
    ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224217.2
    CDS151..3384
    Translation

    Target ORF information:

    RefSeq Version XM_005224160.4
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224160.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCGG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG
    GCCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT
    CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG
    AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC
    TTCTTGCCTG GCTCCACAGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG
    ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA
    ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC
    TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG CGGGGCTCAC CCGGAAGCCC
    TTCCCCAGCG ACGCCGGCGT AGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG
    TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC
    ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC
    GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG
    GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG
    GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG
    ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216402
    Clone ID Related Accession (Same CDS sequence) NM_001143869.2
    Accession Version NM_001143869.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product EMILIN-2 precursor
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from DAAA02056716.1, DAAA02056717.1 and DAAA02056719.1. On Jun 30, 2017 this sequence version replaced NM_001143869.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02403657, SAMN02822088 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACGGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCCGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTGG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137341.1
    CDS1..3222
    Translation

    Target ORF information:

    RefSeq Version NM_001143869.2
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143869.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACGGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGCGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCCGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTGG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCACAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGCGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39599
    Clone ID Related Accession (Same CDS sequence) NM_001143869.1
    Accession Version NM_001143869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product EMILIN-2 precursor
    Comment INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from AAFC03019644.1, AAFC03064819.1 and AAFC03064821.1. On Jan 8, 2009 this sequence version replaced XM_592120.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN01093727 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGTGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCTGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCGCAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGTGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137341.1
    CDS1..3222
    Translation

    Target ORF information:

    RefSeq Version NM_001143869.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA AACGCCTCAG CCTCCACCCC GGGGGAAGAC CCCGGAGGAC
    GTAGGAAAGC CCTCAAGCCC GGCAGAGGCC CCCACGGAGC TGTCCACTCC AGTGCTGACC
    CCACTGCTGC CGCCCAGCAC TCCCTCGCCT CCGAGGGACC CAGGACAGAA GCCCGTCCTG
    CCCAAGAGAC GCCCCCCACC CCTGCGGCCC GGCTGGACAG GGCTGCCCTT CTTGCCTGGC
    TCCACAGGGG TCATCATGGA GACTGGAGAG GCCGGCCCCC CAGGCAGGAT GGGGGTGTCA
    GGGCGGGGCC TGCCCCGGGG TGTGGATGGC CAGACCGGGC AGCGGCCAAT CCCCAGTGCC
    GAGGGCTACG CAGGAGCTCC AGGGTACCCC AAATCACCGC CTGCAGCCTC CCCAGGGGCT
    CCGGTGCCAT CTCTCGTGTC CTTCTCTGTG GGGCTCACCC GGAAGCCCTT CCCCAGCGAC
    GCCGGCGTAG TCCTGTTCAA CAAGGTGCTG GTGAACGACG GGGACGTGTA CGACCCCAGC
    ACCGGGGTCT TCACGGCTCC GTACGACGGG CGCTACCTGA TCACGGCCAC CCTCACACCC
    GAGAGGGATG CGTATGTGGA GGCCGTCCTG TCAGTGGCCA ACGCCAGCGT GGCCCAGCTG
    CACACGGCCG GCTACCGGAG GGAGTTCCTG GAGTACCACC GGCCGCAGGG GGCCCCACAC
    ACCTGCGGGG GCCCGGGCGC CTTCCACCTC ATCGTGCACC TCAAGGCGGG GGACGGTGTC
    AACATCGTGG TCACGGGGGG CCGGCTGGCC CACACGGACT TTGACGAGAT GTACTCCACC
    TTCAGCGGGG TCTTCTTGTA CCCTTTTCTT TCCCACCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32530
    Clone ID Related Accession (Same CDS sequence) XM_005224159.3
    Accession Version XM_005224159.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EMILIN-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224159.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG
    AAGACCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC
    ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA
    CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG
    CCCTTCTTGC CTGGCTCCAC GGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC
    AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG
    CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA
    GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGTGGGGCT CACCCGGAAG
    CCCTTCCCCA GCGACGCCGG CGTGGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC
    GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG
    GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC
    AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA
    CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG
    GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC
    GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224216.1
    CDS421..3657
    Translation

    Target ORF information:

    RefSeq Version XM_005224159.3
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224159.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAC AGAGGACCCC TCAGAAACGC CTCAGCCTCC ACCCCGGGGG
    AAGACCCCGG AGGACGTAGG AAAGCCCTCA AGCCCGGCAG AGGCCCCCAC GGAGCTGTCC
    ACTCCAGTGC TGACCCCACT GCTGCCGCCC AGCACTCCCT CGCCTCCGAG GGACCCAGGA
    CAGAAGCCCG TCCTGCCCAA GAGACGCCCC CCACCCCTGC GGCCCGGCTG GACAGGGCTG
    CCCTTCTTGC CTGGCTCCAC GGGGGTCATC ATGGAGACTG GAGAGGCCGG CCCCCCAGGC
    AGGATGGGGG TGTCAGGGCG GGGCCTGCCC CGGGGTGTGG ATGGCCAGAC CGGGCAGCGG
    CCAATCCCCA GCGCCGAGGG CTACGCAGGA GCTCCAGGGT ACCCCAAATC ACCGCCTGCA
    GCCTCCCCAG GGGCTCCGGT GCCATCTCTC GTGTCCTTCT CCGTGGGGCT CACCCGGAAG
    CCCTTCCCCA GCGACGCCGG CGTGGTCCTG TTCAACAAGG TGCTGGTGAA CGACGGGGAC
    GTGTACGACC CCAGCACCGG GGTCTTCACG GCTCCGTACG ACGGGCGCTA CCTGATCACG
    GCCACCCTCA CACCCGAGAG GGATGCGTAT GTGGAGGCCG TCCTGTCAGT GGCCAACGCC
    AGCGTGGCCC AGCTGCACAC GGCCGGCTAC CGGAGGGAGT TCCTGGAGTA CCACCGGCCA
    CAGGGGGCCC CACACACCTG CGGGGGCCCG GGCGCCTTCC ACCTCATCGT GCACCTCAAG
    GCGGGGGACG GCGTCAACAT CGTGGTCACG GGGGGCCGGC TGGCCCACAC GGACTTTGAC
    GAGATGTACT CCACCTTCAG CGGGGTCTTC TTGTACCCTT TTCTTTCCCA CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32531
    Clone ID Related Accession (Same CDS sequence) XM_005224160.3
    Accession Version XM_005224160.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product EMILIN-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104569.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224160.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG
    ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT
    CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG
    AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC
    TTCTTGCCTG GCTCCACGGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG
    ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA
    ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC
    TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC
    TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG
    TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC
    ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC
    GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG
    GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG
    GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG
    ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224217.1
    CDS1..3234
    Translation

    Target ORF information:

    RefSeq Version XM_005224160.3
    Organism Bos taurus(cattle)
    Definition Bos taurus elastin microfibril interfacer 2 (EMILIN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224160.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTGGCCGC CGACATGGCC CCGGCCCCGG CCCCGGCCCC GCGCGCCCTG GCGCTGGGCG 
    CTGGCACTGC TGGCCCTGGG TGGCGCGGGG CTGTGCCACG CCAGCCCGCA GCCTGGGCAC
    CCCGCACGGC CCAGCGCCAG GAACAAGAAC TGGTGCGCCT ACATCGTGAA CAAGAACGTG
    AGCTGCTCGG TGCTCGAGGG AAGCGAGACT TTTATCCAAG CTCAGTACAA CTGTGGCTGG
    AACCAGAAGC CCTGTCCGTC TGCGCTCATG TATCGCGTGA ATGTCAGACC CAGGTACGTC
    ACCAGGTACA AGACGGTGAC GCAGTTGGAA TGGAGGTGCT GCCCTGGCTT CAGGGGGGGA
    AACTGCCAAG AAGGTCCCAG AGACCCTGGG AGGATGCCCC GCCCCATGCC TGCTCGGCCC
    CGCAACAGCA TGAAGAAACC CACAGACAAT GAACCAAGCC GAGTCTCAGA GCCTAAGAAC
    ACTGTGTCAC CCACTCCTGC AGCAGACCCA AGTCAGACAA GAGATCCAAA ACAGGGGCCC
    CAGGAACTTC AGGAAAAGAA AATTCAGGTG CTGGAGGAGA AGGTTCTCCG GCTCACCAGG
    ACGGTTCTGG ACCTTCAGTC TTCCGTTGCT GGAGTGAGTG AAAACCTCAA ACACGCCGTC
    CAGGACGATG CCAGCAAGAT GCTGGCCTCA TGGCTGAGCA ACCTGCACCC CCGGCCAACG
    CCCATCAGCG CTGTGGGTGG AGAACCAGAA ACAGTTCAGC CCCCCGGCAT CCTCAGCAAT
    AAGGAGTCAG GCATGAAGGA CATCAAGTCT GAACTGGCTG AAGTCAAGGA TGCCCTGAAG
    ACCAAGAGTG ACAAGCTGGA GGAGCTGGAC GGGAAAGTGA AGGGCTATGA AGGGCAGCTC
    AGACAGCTCC AGGAGGCCGC CCAGGGCCCA ACGGTCACCA TGACGACCAG CGAGCTCTTC
    CAAGCCTACG TGGACAGTAA GATCGATGCC CTGAGGGAGG AGCTTATGGA AGGGATGGAC
    CGAAAGCTGG CTGACCTGAA GAACTCGTGT GAGTACAAGT TGATCGGTTT CCAGCAGCAC
    TGTGATGACT ATGGGAGCAG CTACCTGGGA GTGATCGAGC TGATAGGAGA GAAGGAGGCC
    AAGCTGAGAA AAGAAATCAG TGACCTTCAG GCCCGGGTCC AAGACCCTCC AGCCCATGCA
    AACTGCTGTG ACGGTACCAA GCCTGGTGAC TTGGGGCCAC AGATCAAGAT GTTAGACCAG
    AAAATCGAAA GAGTTTCTGA AGCGACAAGA ATGCTGAATG CACGACTAGA CAATGAGTTT
    GACCGCCTCG AGGCTCCAGA GCCGGATGTG GACTTCGATG CCAGATGGCA TGAACTGGAT
    GCAAGGATCA ACGTGACAGA GAGGAATGCA GAAGAGCACT GCTTTTACAT TGAAGAAACC
    CTCCGGGGGA CCATTAGTGG GGAGGTGGAC GGCCTGAGGC AGCTGCTTGA TCAGAAAATA
    CAGTCCCTGG AGGAGCGGCT GGGGAGCGCC CTCCTGGAGA TGGTGAACGG CTCAGAGATG
    GTACCTGCTC CCCCAGTGGC AGCCCCGCCC ACTGTGTCGG GGGCTGGGCC CCCGCAGGTC
    ATGATGGAGT TGAAGCGCCT GAAGGACCAA GTGCGGGTGG TGGAGGACAT GTGCCTGCAG
    AACTTCCGAG GAGAGCCTCG AGGGATGGAA GACACTTTGC GGAACGGGGG CGAGATGGTG
    AGCCTTTTGA GATCTCTAAA CGACACGATG CAGGGGAAGT TTCAGGACAC TGAACGCAGC
    ATCCAGCGAC TTCAGCAGGA CTTCAGTTTT CTTTATTCTT GGCTCAACCA CACAGATGAC
    CACTTGAGAC GTCTACAGAA CGAGCTGAGT GGTGGTAGAG GAGGGAAAAA GGCTGCAGGG
    GGTGGGTGGT CCAAGGTGGG TGAGCCAGGG AGGACGGAGG CGCCCCTGCC GTCTCCCCAG
    GCCCCCCCAG CGCATTGCTG CGACCAGCTG GAGGGCAGGT GGCAGCGGCT GCAGGACCTC
    GTCCTCAGCG AGCTGGACAC TTGTAAGGAG AAGACGCACG GAGTCCAGAG GGAGGTGTCT
    GTGGTAGAGG ACAGGGTGTC TCACGTGGAG AAGACCTGTA GCAAGCTGGA CTCCATCTCT
    GGGAGCCTTC AGAAGATCAA GGAGGGGCTC AACAAGCACG TGAACAGCCT CTGGAGCTGC
    ATCCGGCAGA TGAACGGGAC GCTCCGGACA CACTCCAGAG AGATCTCCAG CCTGAAGAAT
    TCCGTCCAGC AGTTCTACAG CCACGTCTTC CAGATTTCTG CTGACCTGCA AGACCTGATC
    CGGTTTCAGC CATCGGCAAA GGACCCCTCA GAAACGCCTC AGCCTCCACC CCGGGGGAAG
    ACCCCGGAGG ACGTAGGAAA GCCCTCAAGC CCGGCAGAGG CCCCCACGGA GCTGTCCACT
    CCAGTGCTGA CCCCACTGCT GCCGCCCAGC ACTCCCTCGC CTCCGAGGGA CCCAGGACAG
    AAGCCCGTCC TGCCCAAGAG ACGCCCCCCA CCCCTGCGGC CCGGCTGGAC AGGGCTGCCC
    TTCTTGCCTG GCTCCACGGG GGTCATCATG GAGACTGGAG AGGCCGGCCC CCCAGGCAGG
    ATGGGGGTGT CAGGGCGGGG CCTGCCCCGG GGTGTGGATG GCCAGACCGG GCAGCGGCCA
    ATCCCCAGCG CCGAGGGCTA CGCAGGAGCT CCAGGGTACC CCAAATCACC GCCTGCAGCC
    TCCCCAGGGG CTCCGGTGCC ATCTCTCGTG TCCTTCTCCG TGGGGCTCAC CCGGAAGCCC
    TTCCCCAGCG ACGCCGGCGT GGTCCTGTTC AACAAGGTGC TGGTGAACGA CGGGGACGTG
    TACGACCCCA GCACCGGGGT CTTCACGGCT CCGTACGACG GGCGCTACCT GATCACGGCC
    ACCCTCACAC CCGAGAGGGA TGCGTATGTG GAGGCCGTCC TGTCAGTGGC CAACGCCAGC
    GTGGCCCAGC TGCACACGGC CGGCTACCGG AGGGAGTTCC TGGAGTACCA CCGGCCACAG
    GGGGCCCCAC ACACCTGCGG GGGCCCGGGC GCCTTCCACC TCATCGTGCA CCTCAAGGCG
    GGGGACGGCG TCAACATCGT GGTCACGGGG GGCCGGCTGG CCCACACGGA CTTTGACGAG
    ATGTACTCCA CCTTCAGCGG GGTCTTCTTG TACCCTTTTC TTTCCCACCT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.