WWP1 cDNA ORF clone, Bos taurus(cattle)

The following WWP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WWP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa41015 NM_001037463.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa41015 XM_025001522.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa222257 XM_025001519.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222257 XM_025001520.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222258 XM_025001521.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222259 XM_025001523.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222260 XM_025001524.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa222261 XM_025001525.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa222262 XM_025001526.1
Latest version!
Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa41015
    Clone ID Related Accession (Same CDS sequence) XM_025001522.1 , NM_001037463.1
    Accession Version NM_001037463.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC108130.1. On or before Nov 27, 2005 this sequence version replaced XM_865812.1, XM_877106.1, XM_877154.1, XM_877206.1, XM_877254.1, XM_877298.1, XM_877348.1, XM_591536.2, XM_877442.1, XM_877492.1. ##Evidence-Data-START## Transcript exon combination :: BC108130.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001032540.1
    CDS72..2837
    Translation

    Target ORF information:

    RefSeq Version NM_001037463.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001037463.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41015
    Clone ID Related Accession (Same CDS sequence) XM_025001522.1 , NM_001037463.1
    Accession Version XM_025001522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857290.1
    CDS348..3113
    Translation

    Target ORF information:

    RefSeq Version XM_025001522.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222257
    Clone ID Related Accession (Same CDS sequence) XM_025001519.1 , XM_025001520.1
    Accession Version XM_025001519.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGGTCTT TGAATATTTT TTCTGTTCCA
    TTTGTTCCAT TTTTCTTCGG GAACATCAGT CATGGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857287.1
    CDS303..3128
    Translation

    Target ORF information:

    RefSeq Version XM_025001519.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001519.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGGTCTT TGAATATTTT TTCTGTTCCA
    TTTGTTCCAT TTTTCTTCGG GAACATCAGT CATGGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222257
    Clone ID Related Accession (Same CDS sequence) XM_025001519.1 , XM_025001520.1
    Accession Version XM_025001520.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGGTCTT TGAATATTTT TTCTGTTCCA
    TTTGTTCCAT TTTTCTTCGG GAACATCAGT CATGGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857288.1
    CDS41..2866
    Translation

    Target ORF information:

    RefSeq Version XM_025001520.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001520.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CATAGAAGTA
    CAGCAAAATG GTGATGCCTT ACATGAAGAC AGAGAGCCTT CAGCAAGGAC GACCACCAGA
    TTGGCTGCTG AAGATACTAA CGGGATAGAT AATTGTGTAC CTACAAACAC TGTAGTACAA
    AACTCATGCT GTTCATATGT AGTTAATGGG GACAACACAC CATCTCCATC TCAGGTTGCT
    GCCAGACCCA AACACACACC AGCTCCAAAA CCACTCCCAT CTGAGCCTGC CAATGACACT
    GTTAATGGAG ACTCACCCTC ATCTGTACCG ACTGATAATG CTTCCAGCAC GGGTACTGCA
    ATAGGATCTG GAGAACCCGC CTTGTCTTCA AATTGCACTA GTACTCCTGT TGAAGACCCT
    CCAGTTCAAG AAACACTGAC CTCCTCGGAA AACAATGAAT GTATTGTCTC TAACAGTGCA
    GCCTTTGGAT CTGAAGCTCA AAGTACATTA GATCCTTCCA CCTCCAGTTC TGCAAGTAGC
    TCTGCTTTTG AAGCAGCCAA ATTAAGACAG CCCGACGGAT GTGTGGAACC TGTCCGGCAG
    CAGTCTGGAG GTGCCAACAC AGAAACGCTG CCATCAGGGT GGGAACAGAG AAAAGATCCT
    CATGGTAGAA CCTACTACGT GGATCATAAC ACACGGACGA CCACGTGGGA GAGGCCGCAG
    CCTCTGCCTC CAGGTTGGGA AAGAAGAGTC GATGACCGGG GCAGAGTGTA TTACGTGGAC
    CATAACACCA GGACAACCAC CTGGCAGCGG CCCACCATGG AGTCGGTGCG GAACTTTGAG
    CAGTGGCAGT CCCAGCGGAG CCAGCTGCAG GGAGCTATGC AGCAGTTCAA CCAGCGATAC
    CTCTACTCGG CTTCAATGTT AGCGGCAGAA AATGACCCAT ACGGGCCTTT GCCACCAGGC
    TGGGAAAAAA GAGTGGATTC AACAGACAGA GTTTACTTCG TGAATCATAA CACAAAAACA
    ACCCAGTGGG AAGACCCCAG AACTCAAGGT TTGCAGAATG AAGAACCCCT GCCAGAAGGT
    TGGGAAATTA GGTATACCCG GGAAGGTGTG CGGTACTTTG TTGATCATAA TACAAGAACA
    ACAACATTCA AAGATCCTCG CAATGGGAAG TCATCTGTAA CTAAGGGTGG TCCACAGATT
    GCCTATGAAC GCAGCTTCAG GTGGAAACTC GCTCACTTCC GTTATTTGTG CCAGTCTAAT
    GCTTTACCCA GTCACGTAAA GATCAATGTG TCCCGGCAGA CGTTGTTCGA AGATTCCTTC
    CAACAGATTA TGGCATTAAA ACCCTATGAC TTGAGGAGGC GATTATATGT AATATTTAGA
    GGAGAAGAAG GACTTGATTA TGGTGGCCTA GCAAGGTCTT TGAATATTTT TTCTGTTCCA
    TTTGTTCCAT TTTTCTTCGG GAACATCAGT CATGGAGAAT GGTTTTTCTT GCTTTCACAT
    GAAGTTTTGA ACCCCATGTA TTGCTTATTT GAGTATGCTG GCAAGAACAA CTATTGTCTA
    CAGATAAATC CAGCATCAAC TATTAATCCA GACCATCTTT CATACTTTTG TTTCATTGGT
    CGCTTTATTG CTATGGCACT CTTTCATGGG AAGTTTATTG ATACTGGTTT CTCGTTACCC
    TTCTACAAGC GTATGCTAAG TAAAAAACTG ACTATTAAGG ATTTGGAATC GATCGATACT
    GAGTTTTATA ATTCCCTTAT CTGGATAAGA GACAACAACA TTGAGGAATG TGGCTTAGAA
    ATGTACTTTT CTGTTGACAT GGAGATTTTG GGGAAGGTTA CGTCACATGA CCTGAAGTTG
    GGAGGTTCCA ATATCCTAGT GACAGAGGAG AACAAAGATG AATATATTGG TTTAATGACT
    GAGTGGCGTT TTTCTCGAGG AGTACAAGAA CAGACCAAAG CTTTCCTTGA TGGCTTTAAT
    GAGGTTGTTC CCCTTCAGTG GCTGCAGTAC TTTGATGAAA AAGAATTGGA GGTTATGTTG
    TGTGGCATGC AGGAGGTCGA CCTGGCAGAT TGGCAGAGAA ACACTGTGTA TCGACATTAT
    ACCAGAAACA GCAAGCAAAT CATTTGGTTT TGGCAGTTTG TGAAGGAGAC AGACAATGAA
    GTGAGAATGC GACTACTGCA GTTTGTCACT GGAACCTGCC GTTTACCTCT AGGAGGATTT
    GCTGAACTCA TGGGAAGTAA TGGGCCTCAA AAATTTTGCA TTGAAAAAGT TGGCAAAGAT
    ACCTGGTTAC CAAGAAGCCA CACATGTTTT AATCGCTTGG ATCTACCACC ATATAAGAGT
    TATGAACAAC TAAAGGAAAA ACTTCTTTTT GCAATAGAAG AAACAGAGGG ATTTGGACAA
    GAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222258
    Clone ID Related Accession (Same CDS sequence) XM_025001521.1
    Accession Version XM_025001521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CAAAGTACAG
    CAAAATGGTG ATGCCTTACA TGAAGACAGA GAGCCTTCAG CAAGGACGAC CACCAGATTG
    GCTGCTGAAG ATACTAACGG GATAGATAAT TGTGTACCTA CAAACACTGT AGTACAAAAC
    TCATGCTGTT CATATGTAGT TAATGGGGAC AACACACCAT CTCCATCTCA GGTTGCTGCC
    AGACCCAAAC ACACACCAGC TCCAAAACCA CTCCCATCTG AGCCTGCCAA TGACACTGTT
    AATGGAGACT CACCCTCATC TGTACCGACT GATAATGCTT CCAGCACGGG TACTGCAATA
    GGATCTGGAG AACCCGCCTT GTCTTCAAAT TGCACTAGTA CTCCTGTTGA AGACCCTCCA
    GTTCAAGAAA CACTGACCTC CTCGGAAAAC AATGAATGTA TTGTCTCTAA CAGTGCAGCC
    TTTGGATCTG AAGCTCAAAG TACATTAGAT CCTTCCACCT CCAGTTCTGC AAGTAGCTCT
    GCTTTTGAAG CAGCCAAATT AAGACAGCCC GACGGATGTG TGGAACCTGT CCGGCAGCAG
    TCTGGAGGTG CCAACACAGA AACGCTGCCA TCAGGGTGGG AACAGAGAAA AGATCCTCAT
    GGTAGAACCT ACTACGTGGA TCATAACACA CGGACGACCA CGTGGGAGAG GCCGCAGCCT
    CTGCCTCCAG GTTGGGAAAG AAGAGTCGAT GACCGGGGCA GAGTGTATTA CGTGGACCAT
    AACACCAGGA CAACCACCTG GCAGCGGCCC ACCATGGAGT CGGTGCGGAA CTTTGAGCAG
    TGGCAGTCCC AGCGGAGCCA GCTGCAGGGA GCTATGCAGC AGTTCAACCA GCGATACCTC
    TACTCGGCTT CAATGTTAGC GGCAGAAAAT GACCCATACG GGCCTTTGCC ACCAGGCTGG
    GAAAAAAGAG TGGATTCAAC AGACAGAGTT TACTTCGTGA ATCATAACAC AAAAACAACC
    CAGTGGGAAG ACCCCAGAAC TCAAGGTTTG CAGAATGAAG AACCCCTGCC AGAAGGTTGG
    GAAATTAGGT ATACCCGGGA AGGTGTGCGG TACTTTGTTG ATCATAATAC AAGAACAACA
    ACATTCAAAG ATCCTCGCAA TGGGAAGTCA TCTGTAACTA AGGGTGGTCC ACAGATTGCC
    TATGAACGCA GCTTCAGGTG GAAACTCGCT CACTTCCGTT ATTTGTGCCA GTCTAATGCT
    TTACCCAGTC ACGTAAAGAT CAATGTGTCC CGGCAGACGT TGTTCGAAGA TTCCTTCCAA
    CAGATTATGG CATTAAAACC CTATGACTTG AGGAGGCGAT TATATGTAAT ATTTAGAGGA
    GAAGAAGGAC TTGATTATGG TGGCCTAGCA AGGTCTTTGA ATATTTTTTC TGTTCCATTT
    GTTCCATTTT TCTTCGGGAA CATCAGTCAT GGAGAATGGT TTTTCTTGCT TTCACATGAA
    GTTTTGAACC CCATGTATTG CTTATTTGAG TATGCTGGCA AGAACAACTA TTGTCTACAG
    ATAAATCCAG CATCAACTAT TAATCCAGAC CATCTTTCAT ACTTTTGTTT CATTGGTCGC
    TTTATTGCTA TGGCACTCTT TCATGGGAAG TTTATTGATA CTGGTTTCTC GTTACCCTTC
    TACAAGCGTA TGCTAAGTAA AAAACTGACT ATTAAGGATT TGGAATCGAT CGATACTGAG
    TTTTATAATT CCCTTATCTG GATAAGAGAC AACAACATTG AGGAATGTGG CTTAGAAATG
    TACTTTTCTG TTGACATGGA GATTTTGGGG AAGGTTACGT CACATGACCT GAAGTTGGGA
    GGTTCCAATA TCCTAGTGAC AGAGGAGAAC AAAGATGAAT ATATTGGTTT AATGACTGAG
    TGGCGTTTTT CTCGAGGAGT ACAAGAACAG ACCAAAGCTT TCCTTGATGG CTTTAATGAG
    GTTGTTCCCC TTCAGTGGCT GCAGTACTTT GATGAAAAAG AATTGGAGGT TATGTTGTGT
    GGCATGCAGG AGGTCGACCT GGCAGATTGG CAGAGAAACA CTGTGTATCG ACATTATACC
    AGAAACAGCA AGCAAATCAT TTGGTTTTGG CAGTTTGTGA AGGAGACAGA CAATGAAGTG
    AGAATGCGAC TACTGCAGTT TGTCACTGGA ACCTGCCGTT TACCTCTAGG AGGATTTGCT
    GAACTCATGG GAAGTAATGG GCCTCAAAAA TTTTGCATTG AAAAAGTTGG CAAAGATACC
    TGGTTACCAA GAAGCCACAC ATGTTTTAAT CGCTTGGATC TACCACCATA TAAGAGTTAT
    GAACAACTAA AGGAAAAACT TCTTTTTGCA ATAGAAGAAA CAGAGGGATT TGGACAAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857289.1
    CDS303..3125
    Translation

    Target ORF information:

    RefSeq Version XM_025001521.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CAAAGTACAG
    CAAAATGGTG ATGCCTTACA TGAAGACAGA GAGCCTTCAG CAAGGACGAC CACCAGATTG
    GCTGCTGAAG ATACTAACGG GATAGATAAT TGTGTACCTA CAAACACTGT AGTACAAAAC
    TCATGCTGTT CATATGTAGT TAATGGGGAC AACACACCAT CTCCATCTCA GGTTGCTGCC
    AGACCCAAAC ACACACCAGC TCCAAAACCA CTCCCATCTG AGCCTGCCAA TGACACTGTT
    AATGGAGACT CACCCTCATC TGTACCGACT GATAATGCTT CCAGCACGGG TACTGCAATA
    GGATCTGGAG AACCCGCCTT GTCTTCAAAT TGCACTAGTA CTCCTGTTGA AGACCCTCCA
    GTTCAAGAAA CACTGACCTC CTCGGAAAAC AATGAATGTA TTGTCTCTAA CAGTGCAGCC
    TTTGGATCTG AAGCTCAAAG TACATTAGAT CCTTCCACCT CCAGTTCTGC AAGTAGCTCT
    GCTTTTGAAG CAGCCAAATT AAGACAGCCC GACGGATGTG TGGAACCTGT CCGGCAGCAG
    TCTGGAGGTG CCAACACAGA AACGCTGCCA TCAGGGTGGG AACAGAGAAA AGATCCTCAT
    GGTAGAACCT ACTACGTGGA TCATAACACA CGGACGACCA CGTGGGAGAG GCCGCAGCCT
    CTGCCTCCAG GTTGGGAAAG AAGAGTCGAT GACCGGGGCA GAGTGTATTA CGTGGACCAT
    AACACCAGGA CAACCACCTG GCAGCGGCCC ACCATGGAGT CGGTGCGGAA CTTTGAGCAG
    TGGCAGTCCC AGCGGAGCCA GCTGCAGGGA GCTATGCAGC AGTTCAACCA GCGATACCTC
    TACTCGGCTT CAATGTTAGC GGCAGAAAAT GACCCATACG GGCCTTTGCC ACCAGGCTGG
    GAAAAAAGAG TGGATTCAAC AGACAGAGTT TACTTCGTGA ATCATAACAC AAAAACAACC
    CAGTGGGAAG ACCCCAGAAC TCAAGGTTTG CAGAATGAAG AACCCCTGCC AGAAGGTTGG
    GAAATTAGGT ATACCCGGGA AGGTGTGCGG TACTTTGTTG ATCATAATAC AAGAACAACA
    ACATTCAAAG ATCCTCGCAA TGGGAAGTCA TCTGTAACTA AGGGTGGTCC ACAGATTGCC
    TATGAACGCA GCTTCAGGTG GAAACTCGCT CACTTCCGTT ATTTGTGCCA GTCTAATGCT
    TTACCCAGTC ACGTAAAGAT CAATGTGTCC CGGCAGACGT TGTTCGAAGA TTCCTTCCAA
    CAGATTATGG CATTAAAACC CTATGACTTG AGGAGGCGAT TATATGTAAT ATTTAGAGGA
    GAAGAAGGAC TTGATTATGG TGGCCTAGCA AGGTCTTTGA ATATTTTTTC TGTTCCATTT
    GTTCCATTTT TCTTCGGGAA CATCAGTCAT GGAGAATGGT TTTTCTTGCT TTCACATGAA
    GTTTTGAACC CCATGTATTG CTTATTTGAG TATGCTGGCA AGAACAACTA TTGTCTACAG
    ATAAATCCAG CATCAACTAT TAATCCAGAC CATCTTTCAT ACTTTTGTTT CATTGGTCGC
    TTTATTGCTA TGGCACTCTT TCATGGGAAG TTTATTGATA CTGGTTTCTC GTTACCCTTC
    TACAAGCGTA TGCTAAGTAA AAAACTGACT ATTAAGGATT TGGAATCGAT CGATACTGAG
    TTTTATAATT CCCTTATCTG GATAAGAGAC AACAACATTG AGGAATGTGG CTTAGAAATG
    TACTTTTCTG TTGACATGGA GATTTTGGGG AAGGTTACGT CACATGACCT GAAGTTGGGA
    GGTTCCAATA TCCTAGTGAC AGAGGAGAAC AAAGATGAAT ATATTGGTTT AATGACTGAG
    TGGCGTTTTT CTCGAGGAGT ACAAGAACAG ACCAAAGCTT TCCTTGATGG CTTTAATGAG
    GTTGTTCCCC TTCAGTGGCT GCAGTACTTT GATGAAAAAG AATTGGAGGT TATGTTGTGT
    GGCATGCAGG AGGTCGACCT GGCAGATTGG CAGAGAAACA CTGTGTATCG ACATTATACC
    AGAAACAGCA AGCAAATCAT TTGGTTTTGG CAGTTTGTGA AGGAGACAGA CAATGAAGTG
    AGAATGCGAC TACTGCAGTT TGTCACTGGA ACCTGCCGTT TACCTCTAGG AGGATTTGCT
    GAACTCATGG GAAGTAATGG GCCTCAAAAA TTTTGCATTG AAAAAGTTGG CAAAGATACC
    TGGTTACCAA GAAGCCACAC ATGTTTTAAT CGCTTGGATC TACCACCATA TAAGAGTTAT
    GAACAACTAA AGGAAAAACT TCTTTTTGCA ATAGAAGAAA CAGAGGGATT TGGACAAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222259
    Clone ID Related Accession (Same CDS sequence) XM_025001523.1
    Accession Version XM_025001523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2763bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CAAAGTACAG
    CAAAATGGTG ATGCCTTACA TGAAGACAGA GAGCCTTCAG CAAGGACGAC CACCAGATTG
    GCTGCTGAAG ATACTAACGG GATAGATAAT TGTGTACCTA CAAACACTGT AGTACAAAAC
    TCATGCTGTT CATATGTAGT TAATGGGGAC AACACACCAT CTCCATCTCA GGTTGCTGCC
    AGACCCAAAC ACACACCAGC TCCAAAACCA CTCCCATCTG AGCCTGCCAA TGACACTGTT
    AATGGAGACT CACCCTCATC TGTACCGACT GATAATGCTT CCAGCACGGG TACTGCAATA
    GGATCTGGAG AACCCGCCTT GTCTTCAAAT TGCACTAGTA CTCCTGTTGA AGACCCTCCA
    GTTCAAGAAA CACTGACCTC CTCGGAAAAC AATGAATGTA TTGTCTCTAA CAGTGCAGCC
    TTTGGATCTG AAGCTCAAAG TACATTAGAT CCTTCCACCT CCAGTTCTGC AAGTAGCTCT
    GCTTTTGAAG CAGCCAAATT AAGACAGCCC GACGGATGTG TGGAACCTGT CCGGCAGCAG
    TCTGGAGGTG CCAACACAGA AACGCTGCCA TCAGGGTGGG AACAGAGAAA AGATCCTCAT
    GGTAGAACCT ACTACGTGGA TCATAACACA CGGACGACCA CGTGGGAGAG GCCGCAGCCT
    CTGCCTCCAG GTTGGGAAAG AAGAGTCGAT GACCGGGGCA GAGTGTATTA CGTGGACCAT
    AACACCAGGA CAACCACCTG GCAGCGGCCC ACCATGGAGT CGGTGCGGAA CTTTGAGCAG
    TGGCAGTCCC AGCGGAGCCA GCTGCAGGGA GCTATGCAGC AGTTCAACCA GCGATACCTC
    TACTCGGCTT CAATGTTAGC GGCAGAAAAT GACCCATACG GGCCTTTGCC ACCAGGCTGG
    GAAAAAAGAG TGGATTCAAC AGACAGAGTT TACTTCGTGA ATCATAACAC AAAAACAACC
    CAGTGGGAAG ACCCCAGAAC TCAAGGTTTG CAGAATGAAG AACCCCTGCC AGAAGGTTGG
    GAAATTAGGT ATACCCGGGA AGGTGTGCGG TACTTTGTTG ATCATAATAC AAGAACAACA
    ACATTCAAAG ATCCTCGCAA TGGGAAGTCA TCTGTAACTA AGGGTGGTCC ACAGATTGCC
    TATGAACGCA GCTTCAGGTG GAAACTCGCT CACTTCCGTT ATTTGTGCCA GTCTAATGCT
    TTACCCAGTC ACGTAAAGAT CAATGTGTCC CGGCAGACGT TGTTCGAAGA TTCCTTCCAA
    CAGATTATGG CATTAAAACC CTATGACTTG AGGAGGCGAT TATATGTAAT ATTTAGAGGA
    GAAGAAGGAC TTGATTATGG TGGCCTAGCA AGAGAATGGT TTTTCTTGCT TTCACATGAA
    GTTTTGAACC CCATGTATTG CTTATTTGAG TATGCTGGCA AGAACAACTA TTGTCTACAG
    ATAAATCCAG CATCAACTAT TAATCCAGAC CATCTTTCAT ACTTTTGTTT CATTGGTCGC
    TTTATTGCTA TGGCACTCTT TCATGGGAAG TTTATTGATA CTGGTTTCTC GTTACCCTTC
    TACAAGCGTA TGCTAAGTAA AAAACTGACT ATTAAGGATT TGGAATCGAT CGATACTGAG
    TTTTATAATT CCCTTATCTG GATAAGAGAC AACAACATTG AGGAATGTGG CTTAGAAATG
    TACTTTTCTG TTGACATGGA GATTTTGGGG AAGGTTACGT CACATGACCT GAAGTTGGGA
    GGTTCCAATA TCCTAGTGAC AGAGGAGAAC AAAGATGAAT ATATTGGTTT AATGACTGAG
    TGGCGTTTTT CTCGAGGAGT ACAAGAACAG ACCAAAGCTT TCCTTGATGG CTTTAATGAG
    GTTGTTCCCC TTCAGTGGCT GCAGTACTTT GATGAAAAAG AATTGGAGGT TATGTTGTGT
    GGCATGCAGG AGGTCGACCT GGCAGATTGG CAGAGAAACA CTGTGTATCG ACATTATACC
    AGAAACAGCA AGCAAATCAT TTGGTTTTGG CAGTTTGTGA AGGAGACAGA CAATGAAGTG
    AGAATGCGAC TACTGCAGTT TGTCACTGGA ACCTGCCGTT TACCTCTAGG AGGATTTGCT
    GAACTCATGG GAAGTAATGG GCCTCAAAAA TTTTGCATTG AAAAAGTTGG CAAAGATACC
    TGGTTACCAA GAAGCCACAC ATGTTTTAAT CGCTTGGATC TACCACCATA TAAGAGTTAT
    GAACAACTAA AGGAAAAACT TCTTTTTGCA ATAGAAGAAA CAGAGGGATT TGGACAAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857291.1
    CDS303..3065
    Translation

    Target ORF information:

    RefSeq Version XM_025001523.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTGGAAA GAGTGAAAGA ACAATTAAAA
    CTTTGCTTGG AAACCAAGAA TGGTGTCGTG CAAACTGGTG AATTAACAGT TGTGCTTGAT
    GGACTGGTGA TTGAGCAAGA AAATATAACA AACGGCAGCT CATCTCCAGC CAAAGTACAG
    CAAAATGGTG ATGCCTTACA TGAAGACAGA GAGCCTTCAG CAAGGACGAC CACCAGATTG
    GCTGCTGAAG ATACTAACGG GATAGATAAT TGTGTACCTA CAAACACTGT AGTACAAAAC
    TCATGCTGTT CATATGTAGT TAATGGGGAC AACACACCAT CTCCATCTCA GGTTGCTGCC
    AGACCCAAAC ACACACCAGC TCCAAAACCA CTCCCATCTG AGCCTGCCAA TGACACTGTT
    AATGGAGACT CACCCTCATC TGTACCGACT GATAATGCTT CCAGCACGGG TACTGCAATA
    GGATCTGGAG AACCCGCCTT GTCTTCAAAT TGCACTAGTA CTCCTGTTGA AGACCCTCCA
    GTTCAAGAAA CACTGACCTC CTCGGAAAAC AATGAATGTA TTGTCTCTAA CAGTGCAGCC
    TTTGGATCTG AAGCTCAAAG TACATTAGAT CCTTCCACCT CCAGTTCTGC AAGTAGCTCT
    GCTTTTGAAG CAGCCAAATT AAGACAGCCC GACGGATGTG TGGAACCTGT CCGGCAGCAG
    TCTGGAGGTG CCAACACAGA AACGCTGCCA TCAGGGTGGG AACAGAGAAA AGATCCTCAT
    GGTAGAACCT ACTACGTGGA TCATAACACA CGGACGACCA CGTGGGAGAG GCCGCAGCCT
    CTGCCTCCAG GTTGGGAAAG AAGAGTCGAT GACCGGGGCA GAGTGTATTA CGTGGACCAT
    AACACCAGGA CAACCACCTG GCAGCGGCCC ACCATGGAGT CGGTGCGGAA CTTTGAGCAG
    TGGCAGTCCC AGCGGAGCCA GCTGCAGGGA GCTATGCAGC AGTTCAACCA GCGATACCTC
    TACTCGGCTT CAATGTTAGC GGCAGAAAAT GACCCATACG GGCCTTTGCC ACCAGGCTGG
    GAAAAAAGAG TGGATTCAAC AGACAGAGTT TACTTCGTGA ATCATAACAC AAAAACAACC
    CAGTGGGAAG ACCCCAGAAC TCAAGGTTTG CAGAATGAAG AACCCCTGCC AGAAGGTTGG
    GAAATTAGGT ATACCCGGGA AGGTGTGCGG TACTTTGTTG ATCATAATAC AAGAACAACA
    ACATTCAAAG ATCCTCGCAA TGGGAAGTCA TCTGTAACTA AGGGTGGTCC ACAGATTGCC
    TATGAACGCA GCTTCAGGTG GAAACTCGCT CACTTCCGTT ATTTGTGCCA GTCTAATGCT
    TTACCCAGTC ACGTAAAGAT CAATGTGTCC CGGCAGACGT TGTTCGAAGA TTCCTTCCAA
    CAGATTATGG CATTAAAACC CTATGACTTG AGGAGGCGAT TATATGTAAT ATTTAGAGGA
    GAAGAAGGAC TTGATTATGG TGGCCTAGCA AGAGAATGGT TTTTCTTGCT TTCACATGAA
    GTTTTGAACC CCATGTATTG CTTATTTGAG TATGCTGGCA AGAACAACTA TTGTCTACAG
    ATAAATCCAG CATCAACTAT TAATCCAGAC CATCTTTCAT ACTTTTGTTT CATTGGTCGC
    TTTATTGCTA TGGCACTCTT TCATGGGAAG TTTATTGATA CTGGTTTCTC GTTACCCTTC
    TACAAGCGTA TGCTAAGTAA AAAACTGACT ATTAAGGATT TGGAATCGAT CGATACTGAG
    TTTTATAATT CCCTTATCTG GATAAGAGAC AACAACATTG AGGAATGTGG CTTAGAAATG
    TACTTTTCTG TTGACATGGA GATTTTGGGG AAGGTTACGT CACATGACCT GAAGTTGGGA
    GGTTCCAATA TCCTAGTGAC AGAGGAGAAC AAAGATGAAT ATATTGGTTT AATGACTGAG
    TGGCGTTTTT CTCGAGGAGT ACAAGAACAG ACCAAAGCTT TCCTTGATGG CTTTAATGAG
    GTTGTTCCCC TTCAGTGGCT GCAGTACTTT GATGAAAAAG AATTGGAGGT TATGTTGTGT
    GGCATGCAGG AGGTCGACCT GGCAGATTGG CAGAGAAACA CTGTGTATCG ACATTATACC
    AGAAACAGCA AGCAAATCAT TTGGTTTTGG CAGTTTGTGA AGGAGACAGA CAATGAAGTG
    AGAATGCGAC TACTGCAGTT TGTCACTGGA ACCTGCCGTT TACCTCTAGG AGGATTTGCT
    GAACTCATGG GAAGTAATGG GCCTCAAAAA TTTTGCATTG AAAAAGTTGG CAAAGATACC
    TGGTTACCAA GAAGCCACAC ATGTTTTAAT CGCTTGGATC TACCACCATA TAAGAGTTAT
    GAACAACTAA AGGAAAAACT TCTTTTTGCA ATAGAAGAAA CAGAGGGATT TGGACAAGAA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222260
    Clone ID Related Accession (Same CDS sequence) XM_025001524.1
    Accession Version XM_025001524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTAGAAG TACAGCAAAA TGGTGATGCC
    TTACATGAAG ACAGAGAGCC TTCAGCAAGG ACGACCACCA GATTGGCTGC TGAAGATACT
    AACGGGATAG ATAATTGTGT ACCTACAAAC ACTGTAGTAC AAAACTCATG CTGTTCATAT
    GTAGTTAATG GGGACAACAC ACCATCTCCA TCTCAGGTTG CTGCCAGACC CAAACACACA
    CCAGCTCCAA AACCACTCCC ATCTGAGCCT GCCAATGACA CTGTTAATGG AGACTCACCC
    TCATCTGTAC CGACTGATAA TGCTTCCAGC ACGGGTACTG CAATAGGATC TGGAGAACCC
    GCCTTGTCTT CAAATTGCAC TAGTACTCCT GTTGAAGACC CTCCAGTTCA AGAAACACTG
    ACCTCCTCGG AAAACAATGA ATGTATTGTC TCTAACAGTG CAGCCTTTGG ATCTGAAGCT
    CAAAGTACAT TAGATCCTTC CACCTCCAGT TCTGCAAGTA GCTCTGCTTT TGAAGCAGCC
    AAATTAAGAC AGCCCGACGG ATGTGTGGAA CCTGTCCGGC AGCAGTCTGG AGGTGCCAAC
    ACAGAAACGC TGCCATCAGG GTGGGAACAG AGAAAAGATC CTCATGGTAG AACCTACTAC
    GTGGATCATA ACACACGGAC GACCACGTGG GAGAGGCCGC AGCCTCTGCC TCCAGGTTGG
    GAAAGAAGAG TCGATGACCG GGGCAGAGTG TATTACGTGG ACCATAACAC CAGGACAACC
    ACCTGGCAGC GGCCCACCAT GGAGTCGGTG CGGAACTTTG AGCAGTGGCA GTCCCAGCGG
    AGCCAGCTGC AGGGAGCTAT GCAGCAGTTC AACCAGCGAT ACCTCTACTC GGCTTCAATG
    TTAGCGGCAG AAAATGACCC ATACGGGCCT TTGCCACCAG GCTGGGAAAA AAGAGTGGAT
    TCAACAGACA GAGTTTACTT CGTGAATCAT AACACAAAAA CAACCCAGTG GGAAGACCCC
    AGAACTCAAG GTTTGCAGAA TGAAGAACCC CTGCCAGAAG GTTGGGAAAT TAGGTATACC
    CGGGAAGGTG TGCGGTACTT TGTTGATCAT AATACAAGAA CAACAACATT CAAAGATCCT
    CGCAATGGGA AGTCATCTGT AACTAAGGGT GGTCCACAGA TTGCCTATGA ACGCAGCTTC
    AGGTGGAAAC TCGCTCACTT CCGTTATTTG TGCCAGTCTA ATGCTTTACC CAGTCACGTA
    AAGATCAATG TGTCCCGGCA GACGTTGTTC GAAGATTCCT TCCAACAGAT TATGGCATTA
    AAACCCTATG ACTTGAGGAG GCGATTATAT GTAATATTTA GAGGAGAAGA AGGACTTGAT
    TATGGTGGCC TAGCAAGGTC TTTGAATATT TTTTCTGTTC CATTTGTTCC ATTTTTCTTC
    GGGAACATCA GTCATGGAGA ATGGTTTTTC TTGCTTTCAC ATGAAGTTTT GAACCCCATG
    TATTGCTTAT TTGAGTATGC TGGCAAGAAC AACTATTGTC TACAGATAAA TCCAGCATCA
    ACTATTAATC CAGACCATCT TTCATACTTT TGTTTCATTG GTCGCTTTAT TGCTATGGCA
    CTCTTTCATG GGAAGTTTAT TGATACTGGT TTCTCGTTAC CCTTCTACAA GCGTATGCTA
    AGTAAAAAAC TGACTATTAA GGATTTGGAA TCGATCGATA CTGAGTTTTA TAATTCCCTT
    ATCTGGATAA GAGACAACAA CATTGAGGAA TGTGGCTTAG AAATGTACTT TTCTGTTGAC
    ATGGAGATTT TGGGGAAGGT TACGTCACAT GACCTGAAGT TGGGAGGTTC CAATATCCTA
    GTGACAGAGG AGAACAAAGA TGAATATATT GGTTTAATGA CTGAGTGGCG TTTTTCTCGA
    GGAGTACAAG AACAGACCAA AGCTTTCCTT GATGGCTTTA ATGAGGTTGT TCCCCTTCAG
    TGGCTGCAGT ACTTTGATGA AAAAGAATTG GAGGTTATGT TGTGTGGCAT GCAGGAGGTC
    GACCTGGCAG ATTGGCAGAG AAACACTGTG TATCGACATT ATACCAGAAA CAGCAAGCAA
    ATCATTTGGT TTTGGCAGTT TGTGAAGGAG ACAGACAATG AAGTGAGAAT GCGACTACTG
    CAGTTTGTCA CTGGAACCTG CCGTTTACCT CTAGGAGGAT TTGCTGAACT CATGGGAAGT
    AATGGGCCTC AAAAATTTTG CATTGAAAAA GTTGGCAAAG ATACCTGGTT ACCAAGAAGC
    CACACATGTT TTAATCGCTT GGATCTACCA CCATATAAGA GTTATGAACA ACTAAAGGAA
    AAACTTCTTT TTGCAATAGA AGAAACAGAG GGATTTGGAC AAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857292.1
    CDS303..2990
    Translation

    Target ORF information:

    RefSeq Version XM_025001524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGCCACTG CTTCACCAAG GTCTGATACT AGTAATAACC ACAGTGGAAG GTTGCAGTTA 
    CAGGTAACTG TTTCTAGTGC CAAACTAAAA AGAAAAAAGA ACTGGTTTGG AACAGCAATA
    TATACAGAAG TAGCTGCAGA TGGAGAAAGT AAGAAAACAG CAAAATCCAG TAGTTCTTCT
    AATCCAAAAT GGGACGAACA GCTAACTGTA AATGTGACCC CACAGACTAC ATTGGAATTT
    CGAGTTTGGA GTCATCATAC TATAAAAGCA GATGCTTTGT TAGGAAGAGC AACAGTAGAT
    TTGAAACAAG CTCTGTTAAT ACACAATAGA AAATTAGAAG TACAGCAAAA TGGTGATGCC
    TTACATGAAG ACAGAGAGCC TTCAGCAAGG ACGACCACCA GATTGGCTGC TGAAGATACT
    AACGGGATAG ATAATTGTGT ACCTACAAAC ACTGTAGTAC AAAACTCATG CTGTTCATAT
    GTAGTTAATG GGGACAACAC ACCATCTCCA TCTCAGGTTG CTGCCAGACC CAAACACACA
    CCAGCTCCAA AACCACTCCC ATCTGAGCCT GCCAATGACA CTGTTAATGG AGACTCACCC
    TCATCTGTAC CGACTGATAA TGCTTCCAGC ACGGGTACTG CAATAGGATC TGGAGAACCC
    GCCTTGTCTT CAAATTGCAC TAGTACTCCT GTTGAAGACC CTCCAGTTCA AGAAACACTG
    ACCTCCTCGG AAAACAATGA ATGTATTGTC TCTAACAGTG CAGCCTTTGG ATCTGAAGCT
    CAAAGTACAT TAGATCCTTC CACCTCCAGT TCTGCAAGTA GCTCTGCTTT TGAAGCAGCC
    AAATTAAGAC AGCCCGACGG ATGTGTGGAA CCTGTCCGGC AGCAGTCTGG AGGTGCCAAC
    ACAGAAACGC TGCCATCAGG GTGGGAACAG AGAAAAGATC CTCATGGTAG AACCTACTAC
    GTGGATCATA ACACACGGAC GACCACGTGG GAGAGGCCGC AGCCTCTGCC TCCAGGTTGG
    GAAAGAAGAG TCGATGACCG GGGCAGAGTG TATTACGTGG ACCATAACAC CAGGACAACC
    ACCTGGCAGC GGCCCACCAT GGAGTCGGTG CGGAACTTTG AGCAGTGGCA GTCCCAGCGG
    AGCCAGCTGC AGGGAGCTAT GCAGCAGTTC AACCAGCGAT ACCTCTACTC GGCTTCAATG
    TTAGCGGCAG AAAATGACCC ATACGGGCCT TTGCCACCAG GCTGGGAAAA AAGAGTGGAT
    TCAACAGACA GAGTTTACTT CGTGAATCAT AACACAAAAA CAACCCAGTG GGAAGACCCC
    AGAACTCAAG GTTTGCAGAA TGAAGAACCC CTGCCAGAAG GTTGGGAAAT TAGGTATACC
    CGGGAAGGTG TGCGGTACTT TGTTGATCAT AATACAAGAA CAACAACATT CAAAGATCCT
    CGCAATGGGA AGTCATCTGT AACTAAGGGT GGTCCACAGA TTGCCTATGA ACGCAGCTTC
    AGGTGGAAAC TCGCTCACTT CCGTTATTTG TGCCAGTCTA ATGCTTTACC CAGTCACGTA
    AAGATCAATG TGTCCCGGCA GACGTTGTTC GAAGATTCCT TCCAACAGAT TATGGCATTA
    AAACCCTATG ACTTGAGGAG GCGATTATAT GTAATATTTA GAGGAGAAGA AGGACTTGAT
    TATGGTGGCC TAGCAAGGTC TTTGAATATT TTTTCTGTTC CATTTGTTCC ATTTTTCTTC
    GGGAACATCA GTCATGGAGA ATGGTTTTTC TTGCTTTCAC ATGAAGTTTT GAACCCCATG
    TATTGCTTAT TTGAGTATGC TGGCAAGAAC AACTATTGTC TACAGATAAA TCCAGCATCA
    ACTATTAATC CAGACCATCT TTCATACTTT TGTTTCATTG GTCGCTTTAT TGCTATGGCA
    CTCTTTCATG GGAAGTTTAT TGATACTGGT TTCTCGTTAC CCTTCTACAA GCGTATGCTA
    AGTAAAAAAC TGACTATTAA GGATTTGGAA TCGATCGATA CTGAGTTTTA TAATTCCCTT
    ATCTGGATAA GAGACAACAA CATTGAGGAA TGTGGCTTAG AAATGTACTT TTCTGTTGAC
    ATGGAGATTT TGGGGAAGGT TACGTCACAT GACCTGAAGT TGGGAGGTTC CAATATCCTA
    GTGACAGAGG AGAACAAAGA TGAATATATT GGTTTAATGA CTGAGTGGCG TTTTTCTCGA
    GGAGTACAAG AACAGACCAA AGCTTTCCTT GATGGCTTTA ATGAGGTTGT TCCCCTTCAG
    TGGCTGCAGT ACTTTGATGA AAAAGAATTG GAGGTTATGT TGTGTGGCAT GCAGGAGGTC
    GACCTGGCAG ATTGGCAGAG AAACACTGTG TATCGACATT ATACCAGAAA CAGCAAGCAA
    ATCATTTGGT TTTGGCAGTT TGTGAAGGAG ACAGACAATG AAGTGAGAAT GCGACTACTG
    CAGTTTGTCA CTGGAACCTG CCGTTTACCT CTAGGAGGAT TTGCTGAACT CATGGGAAGT
    AATGGGCCTC AAAAATTTTG CATTGAAAAA GTTGGCAAAG ATACCTGGTT ACCAAGAAGC
    CACACATGTT TTAATCGCTT GGATCTACCA CCATATAAGA GTTATGAACA ACTAAAGGAA
    AAACTTCTTT TTGCAATAGA AGAAACAGAG GGATTTGGAC AAGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222261
    Clone ID Related Accession (Same CDS sequence) XM_025001525.1
    Accession Version XM_025001525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2115bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCCATG TTAATGGAGA CTCACCCTCA TCTGTACCGA CTGATAATGC TTCCAGCACG 
    GGTACTGCAA TAGGATCTGG AGAACCCGCC TTGTCTTCAA ATTGCACTAG TACTCCTGTT
    GAAGACCCTC CAGTTCAAGA AACACTGACC TCCTCGGAAA ACAATGAATG TATTGTCTCT
    AACAGTGCAG CCTTTGGATC TGAAGCTCAA AGTACATTAG ATCCTTCCAC CTCCAGTTCT
    GCAAGTAGCT CTGCTTTTGA AGCAGCCAAA TTAAGACAGC CCGACGGATG TGTGGAACCT
    GTCCGGCAGC AGTCTGGAGG TGCCAACACA GAAACGCTGC CATCAGGGTG GGAACAGAGA
    AAAGATCCTC ATGGTAGAAC CTACTACGTG GATCATAACA CACGGACGAC CACGTGGGAG
    AGGCCGCAGC CTCTGCCTCC AGGTTGGGAA AGAAGAGTCG ATGACCGGGG CAGAGTGTAT
    TACGTGGACC ATAACACCAG GACAACCACC TGGCAGCGGC CCACCATGGA GTCGGTGCGG
    AACTTTGAGC AGTGGCAGTC CCAGCGGAGC CAGCTGCAGG GAGCTATGCA GCAGTTCAAC
    CAGCGATACC TCTACTCGGC TTCAATGTTA GCGGCAGAAA ATGACCCATA CGGGCCTTTG
    CCACCAGGCT GGGAAAAAAG AGTGGATTCA ACAGACAGAG TTTACTTCGT GAATCATAAC
    ACAAAAACAA CCCAGTGGGA AGACCCCAGA ACTCAAGGTT TGCAGAATGA AGAACCCCTG
    CCAGAAGGTT GGGAAATTAG GTATACCCGG GAAGGTGTGC GGTACTTTGT TGATCATAAT
    ACAAGAACAA CAACATTCAA AGATCCTCGC AATGGGAAGT CATCTGTAAC TAAGGGTGGT
    CCACAGATTG CCTATGAACG CAGCTTCAGG TGGAAACTCG CTCACTTCCG TTATTTGTGC
    CAGTCTAATG CTTTACCCAG TCACGTAAAG ATCAATGTGT CCCGGCAGAC GTTGTTCGAA
    GATTCCTTCC AACAGATTAT GGCATTAAAA CCCTATGACT TGAGGAGGCG ATTATATGTA
    ATATTTAGAG GAGAAGAAGG ACTTGATTAT GGTGGCCTAG CAAGGTCTTT GAATATTTTT
    TCTGTTCCAT TTGTTCCATT TTTCTTCGGG AACATCAGTC ATGGAGAATG GTTTTTCTTG
    CTTTCACATG AAGTTTTGAA CCCCATGTAT TGCTTATTTG AGTATGCTGG CAAGAACAAC
    TATTGTCTAC AGATAAATCC AGCATCAACT ATTAATCCAG ACCATCTTTC ATACTTTTGT
    TTCATTGGTC GCTTTATTGC TATGGCACTC TTTCATGGGA AGTTTATTGA TACTGGTTTC
    TCGTTACCCT TCTACAAGCG TATGCTAAGT AAAAAACTGA CTATTAAGGA TTTGGAATCG
    ATCGATACTG AGTTTTATAA TTCCCTTATC TGGATAAGAG ACAACAACAT TGAGGAATGT
    GGCTTAGAAA TGTACTTTTC TGTTGACATG GAGATTTTGG GGAAGGTTAC GTCACATGAC
    CTGAAGTTGG GAGGTTCCAA TATCCTAGTG ACAGAGGAGA ACAAAGATGA ATATATTGGT
    TTAATGACTG AGTGGCGTTT TTCTCGAGGA GTACAAGAAC AGACCAAAGC TTTCCTTGAT
    GGCTTTAATG AGGTTGTTCC CCTTCAGTGG CTGCAGTACT TTGATGAAAA AGAATTGGAG
    GTTATGTTGT GTGGCATGCA GGAGGTCGAC CTGGCAGATT GGCAGAGAAA CACTGTGTAT
    CGACATTATA CCAGAAACAG CAAGCAAATC ATTTGGTTTT GGCAGTTTGT GAAGGAGACA
    GACAATGAAG TGAGAATGCG ACTACTGCAG TTTGTCACTG GAACCTGCCG TTTACCTCTA
    GGAGGATTTG CTGAACTCAT GGGAAGTAAT GGGCCTCAAA AATTTTGCAT TGAAAAAGTT
    GGCAAAGATA CCTGGTTACC AAGAAGCCAC ACATGTTTTA ATCGCTTGGA TCTACCACCA
    TATAAGAGTT ATGAACAACT AAAGGAAAAA CTTCTTTTTG CAATAGAAGA AACAGAGGGA
    TTTGGACAAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857293.1
    CDS16..2130
    Translation

    Target ORF information:

    RefSeq Version XM_025001525.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGAGCCATG TTAATGGAGA CTCACCCTCA TCTGTACCGA CTGATAATGC TTCCAGCACG 
    GGTACTGCAA TAGGATCTGG AGAACCCGCC TTGTCTTCAA ATTGCACTAG TACTCCTGTT
    GAAGACCCTC CAGTTCAAGA AACACTGACC TCCTCGGAAA ACAATGAATG TATTGTCTCT
    AACAGTGCAG CCTTTGGATC TGAAGCTCAA AGTACATTAG ATCCTTCCAC CTCCAGTTCT
    GCAAGTAGCT CTGCTTTTGA AGCAGCCAAA TTAAGACAGC CCGACGGATG TGTGGAACCT
    GTCCGGCAGC AGTCTGGAGG TGCCAACACA GAAACGCTGC CATCAGGGTG GGAACAGAGA
    AAAGATCCTC ATGGTAGAAC CTACTACGTG GATCATAACA CACGGACGAC CACGTGGGAG
    AGGCCGCAGC CTCTGCCTCC AGGTTGGGAA AGAAGAGTCG ATGACCGGGG CAGAGTGTAT
    TACGTGGACC ATAACACCAG GACAACCACC TGGCAGCGGC CCACCATGGA GTCGGTGCGG
    AACTTTGAGC AGTGGCAGTC CCAGCGGAGC CAGCTGCAGG GAGCTATGCA GCAGTTCAAC
    CAGCGATACC TCTACTCGGC TTCAATGTTA GCGGCAGAAA ATGACCCATA CGGGCCTTTG
    CCACCAGGCT GGGAAAAAAG AGTGGATTCA ACAGACAGAG TTTACTTCGT GAATCATAAC
    ACAAAAACAA CCCAGTGGGA AGACCCCAGA ACTCAAGGTT TGCAGAATGA AGAACCCCTG
    CCAGAAGGTT GGGAAATTAG GTATACCCGG GAAGGTGTGC GGTACTTTGT TGATCATAAT
    ACAAGAACAA CAACATTCAA AGATCCTCGC AATGGGAAGT CATCTGTAAC TAAGGGTGGT
    CCACAGATTG CCTATGAACG CAGCTTCAGG TGGAAACTCG CTCACTTCCG TTATTTGTGC
    CAGTCTAATG CTTTACCCAG TCACGTAAAG ATCAATGTGT CCCGGCAGAC GTTGTTCGAA
    GATTCCTTCC AACAGATTAT GGCATTAAAA CCCTATGACT TGAGGAGGCG ATTATATGTA
    ATATTTAGAG GAGAAGAAGG ACTTGATTAT GGTGGCCTAG CAAGGTCTTT GAATATTTTT
    TCTGTTCCAT TTGTTCCATT TTTCTTCGGG AACATCAGTC ATGGAGAATG GTTTTTCTTG
    CTTTCACATG AAGTTTTGAA CCCCATGTAT TGCTTATTTG AGTATGCTGG CAAGAACAAC
    TATTGTCTAC AGATAAATCC AGCATCAACT ATTAATCCAG ACCATCTTTC ATACTTTTGT
    TTCATTGGTC GCTTTATTGC TATGGCACTC TTTCATGGGA AGTTTATTGA TACTGGTTTC
    TCGTTACCCT TCTACAAGCG TATGCTAAGT AAAAAACTGA CTATTAAGGA TTTGGAATCG
    ATCGATACTG AGTTTTATAA TTCCCTTATC TGGATAAGAG ACAACAACAT TGAGGAATGT
    GGCTTAGAAA TGTACTTTTC TGTTGACATG GAGATTTTGG GGAAGGTTAC GTCACATGAC
    CTGAAGTTGG GAGGTTCCAA TATCCTAGTG ACAGAGGAGA ACAAAGATGA ATATATTGGT
    TTAATGACTG AGTGGCGTTT TTCTCGAGGA GTACAAGAAC AGACCAAAGC TTTCCTTGAT
    GGCTTTAATG AGGTTGTTCC CCTTCAGTGG CTGCAGTACT TTGATGAAAA AGAATTGGAG
    GTTATGTTGT GTGGCATGCA GGAGGTCGAC CTGGCAGATT GGCAGAGAAA CACTGTGTAT
    CGACATTATA CCAGAAACAG CAAGCAAATC ATTTGGTTTT GGCAGTTTGT GAAGGAGACA
    GACAATGAAG TGAGAATGCG ACTACTGCAG TTTGTCACTG GAACCTGCCG TTTACCTCTA
    GGAGGATTTG CTGAACTCAT GGGAAGTAAT GGGCCTCAAA AATTTTGCAT TGAAAAAGTT
    GGCAAAGATA CCTGGTTACC AAGAAGCCAC ACATGTTTTA ATCGCTTGGA TCTACCACCA
    TATAAGAGTT ATGAACAACT AAAGGAAAAA CTTCTTTTTG CAATAGAAGA AACAGAGGGA
    TTTGGACAAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222262
    Clone ID Related Accession (Same CDS sequence) XM_025001526.1
    Accession Version XM_025001526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product NEDD4-like E3 ubiquitin-protein ligase WWP1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATTCAG TTAATGGAGA CTCACCCTCA TCTGTACCGA CTGATAATGC TTCCAGCACG 
    GGTACTGCAA TAGGATCTGG AGAACCCGCC TTGTCTTCAA ATTGCACTAG TACTCCTGTT
    GAAGACCCTC CAGTTCAAGA AACACTGACC TCCTCGGAAA ACAATGAATG TATTGTCTCT
    AACAGTGCAG CCTTTGGATC TGAAGCTCAA AGTACATTAG ATCCTTCCAC CTCCAGTTCT
    GCAAGTAGCT CTGCTTTTGA AGCAGCCAAA TTAAGACAGC CCGACGGATG TGTGGAACCT
    GTCCGGCAGC AGTCTGGAGG TGCCAACACA GAAACGCTGC CATCAGGGTG GGAACAGAGA
    AAAGATCCTC ATGGTAGAAC CTACTACGTG GATCATAACA CACGGACGAC CACGTGGGAG
    AGGCCGCAGC CTCTGCCTCC AGGTTGGGAA AGAAGAGTCG ATGACCGGGG CAGAGTGTAT
    TACGTGGACC ATAACACCAG GACAACCACC TGGCAGCGGC CCACCATGGA GTCGGTGCGG
    AACTTTGAGC AGTGGCAGTC CCAGCGGAGC CAGCTGCAGG GAGCTATGCA GCAGTTCAAC
    CAGCGATACC TCTACTCGGC TTCAATGTTA GCGGCAGAAA ATGACCCATA CGGGCCTTTG
    CCACCAGGCT GGGAAAAAAG AGTGGATTCA ACAGACAGAG TTTACTTCGT GAATCATAAC
    ACAAAAACAA CCCAGTGGGA AGACCCCAGA ACTCAAGGTT TGCAGAATGA AGAACCCCTG
    CCAGAAGGTT GGGAAATTAG GTATACCCGG GAAGGTGTGC GGTACTTTGT TGATCATAAT
    ACAAGAACAA CAACATTCAA AGATCCTCGC AATGGGAAGT CATCTGTAAC TAAGGGTGGT
    CCACAGATTG CCTATGAACG CAGCTTCAGG TGGAAACTCG CTCACTTCCG TTATTTGTGC
    CAGTCTAATG CTTTACCCAG TCACGTAAAG ATCAATGTGT CCCGGCAGAC GTTGTTCGAA
    GATTCCTTCC AACAGATTAT GGCATTAAAA CCCTATGACT TGAGGAGGCG ATTATATGTA
    ATATTTAGAG GAGAAGAAGG ACTTGATTAT GGTGGCCTAG CAAGAGAATG GTTTTTCTTG
    CTTTCACATG AAGTTTTGAA CCCCATGTAT TGCTTATTTG AGTATGCTGG CAAGAACAAC
    TATTGTCTAC AGATAAATCC AGCATCAACT ATTAATCCAG ACCATCTTTC ATACTTTTGT
    TTCATTGGTC GCTTTATTGC TATGGCACTC TTTCATGGGA AGTTTATTGA TACTGGTTTC
    TCGTTACCCT TCTACAAGCG TATGCTAAGT AAAAAACTGA CTATTAAGGA TTTGGAATCG
    ATCGATACTG AGTTTTATAA TTCCCTTATC TGGATAAGAG ACAACAACAT TGAGGAATGT
    GGCTTAGAAA TGTACTTTTC TGTTGACATG GAGATTTTGG GGAAGGTTAC GTCACATGAC
    CTGAAGTTGG GAGGTTCCAA TATCCTAGTG ACAGAGGAGA ACAAAGATGA ATATATTGGT
    TTAATGACTG AGTGGCGTTT TTCTCGAGGA GTACAAGAAC AGACCAAAGC TTTCCTTGAT
    GGCTTTAATG AGGTTGTTCC CCTTCAGTGG CTGCAGTACT TTGATGAAAA AGAATTGGAG
    GTTATGTTGT GTGGCATGCA GGAGGTCGAC CTGGCAGATT GGCAGAGAAA CACTGTGTAT
    CGACATTATA CCAGAAACAG CAAGCAAATC ATTTGGTTTT GGCAGTTTGT GAAGGAGACA
    GACAATGAAG TGAGAATGCG ACTACTGCAG TTTGTCACTG GAACCTGCCG TTTACCTCTA
    GGAGGATTTG CTGAACTCAT GGGAAGTAAT GGGCCTCAAA AATTTTGCAT TGAAAAAGTT
    GGCAAAGATA CCTGGTTACC AAGAAGCCAC ACATGTTTTA ATCGCTTGGA TCTACCACCA
    TATAAGAGTT ATGAACAACT AAAGGAAAAA CTTCTTTTTG CAATAGAAGA AACAGAGGGA
    TTTGGACAAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857294.1
    CDS65..2119
    Translation

    Target ORF information:

    RefSeq Version XM_025001526.1
    Organism Bos taurus(cattle)
    Definition Bos taurus WW domain containing E3 ubiquitin protein ligase 1 (WWP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGAATTCAG TTAATGGAGA CTCACCCTCA TCTGTACCGA CTGATAATGC TTCCAGCACG 
    GGTACTGCAA TAGGATCTGG AGAACCCGCC TTGTCTTCAA ATTGCACTAG TACTCCTGTT
    GAAGACCCTC CAGTTCAAGA AACACTGACC TCCTCGGAAA ACAATGAATG TATTGTCTCT
    AACAGTGCAG CCTTTGGATC TGAAGCTCAA AGTACATTAG ATCCTTCCAC CTCCAGTTCT
    GCAAGTAGCT CTGCTTTTGA AGCAGCCAAA TTAAGACAGC CCGACGGATG TGTGGAACCT
    GTCCGGCAGC AGTCTGGAGG TGCCAACACA GAAACGCTGC CATCAGGGTG GGAACAGAGA
    AAAGATCCTC ATGGTAGAAC CTACTACGTG GATCATAACA CACGGACGAC CACGTGGGAG
    AGGCCGCAGC CTCTGCCTCC AGGTTGGGAA AGAAGAGTCG ATGACCGGGG CAGAGTGTAT
    TACGTGGACC ATAACACCAG GACAACCACC TGGCAGCGGC CCACCATGGA GTCGGTGCGG
    AACTTTGAGC AGTGGCAGTC CCAGCGGAGC CAGCTGCAGG GAGCTATGCA GCAGTTCAAC
    CAGCGATACC TCTACTCGGC TTCAATGTTA GCGGCAGAAA ATGACCCATA CGGGCCTTTG
    CCACCAGGCT GGGAAAAAAG AGTGGATTCA ACAGACAGAG TTTACTTCGT GAATCATAAC
    ACAAAAACAA CCCAGTGGGA AGACCCCAGA ACTCAAGGTT TGCAGAATGA AGAACCCCTG
    CCAGAAGGTT GGGAAATTAG GTATACCCGG GAAGGTGTGC GGTACTTTGT TGATCATAAT
    ACAAGAACAA CAACATTCAA AGATCCTCGC AATGGGAAGT CATCTGTAAC TAAGGGTGGT
    CCACAGATTG CCTATGAACG CAGCTTCAGG TGGAAACTCG CTCACTTCCG TTATTTGTGC
    CAGTCTAATG CTTTACCCAG TCACGTAAAG ATCAATGTGT CCCGGCAGAC GTTGTTCGAA
    GATTCCTTCC AACAGATTAT GGCATTAAAA CCCTATGACT TGAGGAGGCG ATTATATGTA
    ATATTTAGAG GAGAAGAAGG ACTTGATTAT GGTGGCCTAG CAAGAGAATG GTTTTTCTTG
    CTTTCACATG AAGTTTTGAA CCCCATGTAT TGCTTATTTG AGTATGCTGG CAAGAACAAC
    TATTGTCTAC AGATAAATCC AGCATCAACT ATTAATCCAG ACCATCTTTC ATACTTTTGT
    TTCATTGGTC GCTTTATTGC TATGGCACTC TTTCATGGGA AGTTTATTGA TACTGGTTTC
    TCGTTACCCT TCTACAAGCG TATGCTAAGT AAAAAACTGA CTATTAAGGA TTTGGAATCG
    ATCGATACTG AGTTTTATAA TTCCCTTATC TGGATAAGAG ACAACAACAT TGAGGAATGT
    GGCTTAGAAA TGTACTTTTC TGTTGACATG GAGATTTTGG GGAAGGTTAC GTCACATGAC
    CTGAAGTTGG GAGGTTCCAA TATCCTAGTG ACAGAGGAGA ACAAAGATGA ATATATTGGT
    TTAATGACTG AGTGGCGTTT TTCTCGAGGA GTACAAGAAC AGACCAAAGC TTTCCTTGAT
    GGCTTTAATG AGGTTGTTCC CCTTCAGTGG CTGCAGTACT TTGATGAAAA AGAATTGGAG
    GTTATGTTGT GTGGCATGCA GGAGGTCGAC CTGGCAGATT GGCAGAGAAA CACTGTGTAT
    CGACATTATA CCAGAAACAG CAAGCAAATC ATTTGGTTTT GGCAGTTTGT GAAGGAGACA
    GACAATGAAG TGAGAATGCG ACTACTGCAG TTTGTCACTG GAACCTGCCG TTTACCTCTA
    GGAGGATTTG CTGAACTCAT GGGAAGTAAT GGGCCTCAAA AATTTTGCAT TGAAAAAGTT
    GGCAAAGATA CCTGGTTACC AAGAAGCCAC ACATGTTTTA ATCGCTTGGA TCTACCACCA
    TATAAGAGTT ATGAACAACT AAAGGAAAAA CTTCTTTTTG CAATAGAAGA AACAGAGGGA
    TTTGGACAAG AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.