MYOF cDNA ORF clone, Bos taurus(cattle)

The following MYOF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYOF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa230835 XM_005225523.4
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa33557 XM_005225523.3
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa48677 XM_010819886.1
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa216187 NM_001193212.2
Latest version!
Bos taurus myoferlin (MYOF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1161.30
$1659.00
OBa239152 NM_001193212.3
Latest version!
Bos taurus myoferlin (MYOF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa230831 XM_024985587.1
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa230832 XM_024985588.1
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa230833 XM_024985589.1
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa230834 XM_024985591.1
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa40769 NM_001193212.1
Latest version!
Bos taurus myoferlin (MYOF), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa33555 XM_005225521.3
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa48674 XM_010819883.2
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa48675 XM_010819884.2
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa48676 XM_010819885.2
Latest version!
Bos taurus myoferlin (MYOF), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa230835
    Clone ID Related Accession (Same CDS sequence) XM_005225523.4
    Accession Version XM_005225523.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005225523.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225580.2
    CDS251..5443
    Translation

    Target ORF information:

    RefSeq Version XM_005225523.4
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225523.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33557
    Clone ID Related Accession (Same CDS sequence) XM_005225523.3
    Accession Version XM_005225523.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5193bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225523.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225580.1
    CDS248..5440
    Translation

    Target ORF information:

    RefSeq Version XM_005225523.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225523.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGGTGTC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48677
    Clone ID Related Accession (Same CDS sequence) XM_010819886.1
    Accession Version XM_010819886.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGTGACC AGAGCAGATC ACTGCCCTAC AAACTGGTCT CCCTGCTAAA CGAAAGGGGA 
    CAAGAGACTG GGGCCACAAT TGACCTGGTG ATTGGCTATC ATCCACCTTC AGCTCCACAT
    CCAAATGACC CCGGTGGGAC CAGCGTGCCA GGCACAGGAG AAGAAGAGGA AGATGGAGGT
    GATGAGGACA TGTTGGACAG TACGGTCAGG GGCCCAGGGC CCAGGGGGCC AGGAGGGACC
    GTGCCGGAAG CTCACCTTGC CCGGAGGCTC ACGAAAGCGA AGAGCAGCCG CCGCATGCTG
    TCTAACAAGC CACAGGACTT CCAGATCCGG GTCCGAGTGA TCGAGGGCCG GCAGTTGAGT
    GGCAACAACA TCAAGCCTGT GGTCAAAGTC CACATCTGTG GCCAGACGCA TCGAACAAGA
    ATCAAGCGAG GGAACAATCC ATTTTTTGAT GAGTTATTTT TCTACAATGT CCACCTGACT
    CCTTCTGAAC TGATGGATGA GATCATCAGC ATTCGGGTTT ACAATTCCCA TTCTCTGCGG
    GCAGATTCTC TGATGGGGGA GTTTAAGATC GATGTTGGAT TTGTTTATGA TGAACCTGGT
    CATGCTGTCA TGAGGAAATG GCTTCTCCTC AATGACCCTG AAGATACCAG CTCGGGTGCT
    AAAGGCTACC TGAAAGTCAG CATGTTCGTC CTGGGAACTG GAGACGAGCC TCCTCCTGAG
    AAACAAGATC GTGATAATGA CAGTGATGAC GTGGAAAGTA ACTTGTTACT GCCTGCTGGC
    ATTGCCCTGC GGTGGGTGAC CTTCCTGCTG AAGATCTACA GAGCGGAGGA CATCCCCCAG
    ATGGATGATG CCTTCTCACA GACAGTGAAG GAAATATTTG GAGGCGTCGC TGATAAGAAG
    AACCTAGTGG ACCCTTTTGT AGAAGTTTCC TTTGCCGGAA AAAAGGTTTG CACGAACATA
    ATTGAGAAAA ATGCAAACCC TGAGTGGAAT CAAGTCGTTA ATCTTCAGAT CAAGTTTCCT
    TCAGTGTGTG AAAAAATAAA ACTGACAATA TATGACTGGG ACCGTCTTAC CAAAAATGAT
    GTTGTTGGAA CAACGTATCT GCACCTCTCT AAAATTGCTG CCTCTGGTGG GGAAGTGGAA
    GATTTCTCAT CCTCGGGATC TGGGGCTGCA TCGTATACAG CGAATACAGG AGAAACAGAG
    GTGGGCTTTG TTCCAACTTT TGGACCTTGT TATCTGAACC TTTATGGAAG CCCCCGAGAG
    TACACGGGAT TCCCAGATCC CTATGATGAG CTAAATTCTG GAAAGGGGGA AGGAGTTGCC
    TACAGAGGCA GAATCTTGGT TGAATTAAGC ACTTTACTTG AAAAGACACC ACCAGATAAA
    AAGCTTGAGT CCATTTCGCA TGATGACCTG CTGGTTGTTG AGAAATATCA GCGAAGGCGG
    AAGTACAGCC TGTCTGCTGT GTTCCATTCA GCCACCATGT TGCAAGACAT TGGCGAGGCC
    ATTCAGTTTG AAGTCAGCAT CGGGAACTAC GGCAACAAGT TTGACACGAC TTGTAAGCCT
    CTGGCATCAA CCACTCAGTA TAGCCGTGCT GTATTTGATG GAAACTACTA TTACTACTTG
    CCTTGGGCCC ACACGAAGCC AGTTGTTACA CTGACTTCCT ACTGGGAGGA TATTAGTCAT
    CGCCTGGATG CTGTGAACAC GCTCCTAGTC ATGGCAGAAA GGCTGCAATC AAACATAGAG
    GCTCTAAAAG CAGGGATACA AGGTAAAATT CCTTCAAACG AACTGGCAAA AATGTGGCAT
    AAGCTCATGG ATGAAGTTAT CGAAGACACA AGATACACGT TACCTCTCAC AGAAGGAAAA
    GCTAATGTCA CGGTTCTCGA TACTCAGATC CGAAAGTTGC GATCCAGAGC TCTCTCTCAG
    ATACATGAGG CAGCTGTGAG GATGCGGTCT GAGGTCCCAG ACGTCAAGTC CACATTGGTG
    GAAATTGAGG ACTGGCTCGA TAAATTAATG CAGCTGACTG AGGAGCCTCA GAACAGCATG
    CCTGACATCA TCATCTGGAT GATCCGGGGA GAGAAGAGAT TGGCCTACGC ACGCATTCCC
    GCGCATCAGG TTTTATACTC CACCAGCGGC GGGGATGCGT CTGGGAAGTA CTGTGGGAAA
    ACCCAGACCA TCTTTCTGAA GTATCCACAA GAAAAAAACA ACGGACCAAA GGTGCCCGTG
    GAGTTGCGTG TGAACATCTG GCTGGGCTTA AGTGCTGTTG AGAAGAAGTT CAATAGCTTC
    GCAGAAGGAA CATTCACCGT CTTTGCTGAA ATGTATGAAA ATCAAGCTCT CATGTTTGGA
    AAATGGGGCA CTTCCGGATT AGTGGGTCGT CATAAATTTT CCGATGTCAC AGGAAAAATA
    AAACTCAAGA GGGAATTCTT TTTGCCTCCA AAAGGCTGGG AATGGGAAGG AGACTGGATA
    GTTGATCCTG AAAGAAGCTT GCTGACCGAG GCGGATGCGG GTCACACAGA GTTCACAGAT
    GAAGTGTACC AGAACGAGAG TCGCTACCCC GGGGGCGAGT GGAAGCCGGC GGAGGACACC
    TACACCGATG CGAACGGCGA TAAAGCAGCA TCACCAAGCG AGCTCACTTG CCCTCCAGGC
    TGGGAATGGG AGGATGATGC ATGGGTCTAT GACATTAATC GTGCAGTGGA TGAGAGAGGC
    TGGGAATATG GAATTACCAT TCCTCCTGAT AATAAGCCCA AATCCTGGGT TGCAGCAGAA
    AAAATGTATC ATACTCATAG ACGCCGAAGG CTGGTCCGAA AACGCAAGAA AGATTTAACA
    CAATCCACTT CGAGTACCGC AAGGGCCATG GCAGAATTTG AAGACCGGGA GGGCTGGGAA
    TACGCTTCTC TAATTGGCTG GAAATTTCAC TGGAAACAGC GTAGTTCAGA TACGTTTCGC
    CGCAGACGCT GGAGGAGAAA AATGGCTCCT TCAGAAACAC ATGGTGCAGC TGCCATCTTT
    AAACTTGAAG GGGCCCTTGG CGCAGACACT ACGGACGATG GAGAGGAAAA GAGCGCAGAA
    AAGCAGAAGC ACAGCGCCAC CACTGTATTT GGAGCAAACA CTCCCATTGT TTCCTGTAAT
    TTTGACAGAG TCTACATTTA CCACCTGCGC TGCTACATCT ATCAAGCCAG GAACCTCATG
    GCTCTAGACA AGGATAGCTT TTCAGATCCA TATGCTCATG TCTCCTTCCT TCATCGAAGT
    AAAACCACCG AGATCATCCA CTCAACTCTG AATCCGACAT GGGACCAAAC GATTATATTC
    GATGAAATTG AAATTTATGG GGAACCCCAA ACCATCCTAC AGAACCCACC CAAGGTTATC
    ATCGAACTTT TTGACAATGA CCAAGTGGGC AAAGATGAAT TTTTAGGACG AAGCATGTGC
    TCTCCTCTGG TGAAACTAGA CTCAGAAATG GACATCACTC CCAAACTTCT CTGGTACCCA
    GTGATGAATG GAGACAAAAC CTGTGGGGAT GTCCTTGTAA CTGCAGAGTT GATTCTGAGG
    CACAAGGATG GCTCCAACCT TCCCATTCTT CCCTCGCAAA GGGCACCAAA TCTGTACATG
    GTCCCCCAGG GCATCAGGCC TGTGGTTCAG CTCACTGCTA TTGAGATTCT AGCTTGGGGC
    TTAAGAAATA TGAAGAATTA CCAGATGGCT TCTGTCACCT CCCCCAGTCT CATTGTGGAG
    TGTGGAGGGG AAAGGGTCGA GTCAGTGGTG ATCAAAAACC TGAAGAAGAC ACCCAACTTC
    CCAAGTTCTG TTCTCTTCAT GAGAGTGTTC TTGCCCAAGG AGGAACTGTA CATGCCTCCA
    CTGGTGATCA AGGTCATCGA CCACCGGCAG TTCGGGCGCA AGCCCGTTGT GGGCCAGTGC
    ACCATTGATC GCCTGGACCG CTTTCGCTGT GACCCTTATG CAGGAAAGGA GGACATCGTG
    CCACAGCTAA AAGCCTCCTT TTTGTCTACC CCACCCCGCC GGGACATTGT TATTGAAATA
    GAGGACACCA AGCCATTACT AGCTTCAAAG TGCTTAAGCA GTATGTCAAC AGCACTCAGC
    AAAATGGCTT CTCCAAGGAC AGTGCATCTA ACAGAAAAGG AGGAAGAAAT TGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGGGAA CATGAAAAAT GTGGACAATA TATTCAGAAA
    GGTTATTCCA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAGAGGA ATTCGAGGGC
    CTGACGGACT TCTCAGATAC TTTCAAGCTG TTTCGAGGCA AATCAGATGA GAATGAAGAC
    CCTTCTGTGG TAGGGGAGTT TAAGGGCTCC TTCCGAATCT ACCCACTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCCG GCAGTTTCGG GAGTTACCTG ACAGCGTCCC GCAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTAA TTGAAGACCG AGACAACTAC
    ATCCCCAACA CTCTCAACCC GGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAGG ACCTGAAAAT TTCTGTCTAC GATTATGACA CCTTTACCCG TGATGAAAAA
    GTAGGAGAAA CGATCATTGA CCTAGAAAAC CGATTCCTTT CCCGGTTTGG GTCCCACTGT
    GGCATTCCTG AGCAGTACTG CGTTTCTGGA GTAAACACCT GGCGAGATCA ATTGAAACCA
    ACACAGTTGC TTCAAAATGT GGCCAGATTC AAAGGCTTCC CACCGCCCGT TCTTTCTGAA
    GATGGAAGTA GCATCAGATA TGGAGGAAAA GACTACGGCC TAGATGAATT TGAAGCAAAC
    AAAATCCTGC ACCAGCACCT TGGGCCCCCT GAGGAGCGCC TTGCCCTCTA CATCCTCAGG
    ACTCAGGGGC TGGTTCCTGA ACACGTGGAA ACGAGGACTT TGCACAGCAG CTTCCAGCCT
    AACATCTCCC AGGGAAGACT TCAGATGTGG GTGGATGTTT TCCCCAAGAA TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATT
    ATCTGGAACA CCAAGGATGT TGTCCTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGC
    GACATCTATG TGAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGATGG CGTAGGGAAC TTTAACTGGA GATTCGTCTT CCCTTTTGAC
    TACCTCCCAG CTGAACAGCT CTGTCTCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG GTCATTCAGA TATGGGACAA TGACAAGTTT
    TCTCTGGATG ACTACTTGGG TTTCCTGGAA CTTGATTTGC ATCATACCAT CATTCCAGCA
    AAGTCACCAG AGAAATGCAG TTTGAACATG ATTCCAGACC TCAAAGCCAT GAACCCCCTG
    AAAGTGAAAA CTGCCTCTCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCTTGCTAC
    ACAGAGAAAG ATGGCTCCCG TGTGATGGCT GGGAAAGTGG AGATGACGCT GGAAGTCCTC
    AATGAGAAGG AGGCCGACGA GAGGCCAGCT GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCGTGC
    AAGACCATGA AGTTCATCGT GTGGCGCCGA TTTAAGTGGG TCATCATTGC CCTGCTGATC
    CTGCTGATCC TGCTGTTCTT TGTGGCTGTG TTTCTGTATT CCTTACCGAA CTATTTGTCA
    ATGAAGATTG TGAAACCAAA TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818188.1
    CDS228..6194
    Translation

    Target ORF information:

    RefSeq Version XM_010819886.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819886.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGGTGACC AGAGCAGATC ACTGCCCTAC AAACTGGTCT CCCTGCTAAA CGAAAGGGGA 
    CAAGAGACTG GGGCCACAAT TGACCTGGTG ATTGGCTATC ATCCACCTTC AGCTCCACAT
    CCAAATGACC CCGGTGGGAC CAGCGTGCCA GGCACAGGAG AAGAAGAGGA AGATGGAGGT
    GATGAGGACA TGTTGGACAG TACGGTCAGG GGCCCAGGGC CCAGGGGGCC AGGAGGGACC
    GTGCCGGAAG CTCACCTTGC CCGGAGGCTC ACGAAAGCGA AGAGCAGCCG CCGCATGCTG
    TCTAACAAGC CACAGGACTT CCAGATCCGG GTCCGAGTGA TCGAGGGCCG GCAGTTGAGT
    GGCAACAACA TCAAGCCTGT GGTCAAAGTC CACATCTGTG GCCAGACGCA TCGAACAAGA
    ATCAAGCGAG GGAACAATCC ATTTTTTGAT GAGTTATTTT TCTACAATGT CCACCTGACT
    CCTTCTGAAC TGATGGATGA GATCATCAGC ATTCGGGTTT ACAATTCCCA TTCTCTGCGG
    GCAGATTCTC TGATGGGGGA GTTTAAGATC GATGTTGGAT TTGTTTATGA TGAACCTGGT
    CATGCTGTCA TGAGGAAATG GCTTCTCCTC AATGACCCTG AAGATACCAG CTCGGGTGCT
    AAAGGCTACC TGAAAGTCAG CATGTTCGTC CTGGGAACTG GAGACGAGCC TCCTCCTGAG
    AAACAAGATC GTGATAATGA CAGTGATGAC GTGGAAAGTA ACTTGTTACT GCCTGCTGGC
    ATTGCCCTGC GGTGGGTGAC CTTCCTGCTG AAGATCTACA GAGCGGAGGA CATCCCCCAG
    ATGGATGATG CCTTCTCACA GACAGTGAAG GAAATATTTG GAGGCGTCGC TGATAAGAAG
    AACCTAGTGG ACCCTTTTGT AGAAGTTTCC TTTGCCGGAA AAAAGGTTTG CACGAACATA
    ATTGAGAAAA ATGCAAACCC TGAGTGGAAT CAAGTCGTTA ATCTTCAGAT CAAGTTTCCT
    TCAGTGTGTG AAAAAATAAA ACTGACAATA TATGACTGGG ACCGTCTTAC CAAAAATGAT
    GTTGTTGGAA CAACGTATCT GCACCTCTCT AAAATTGCTG CCTCTGGTGG GGAAGTGGAA
    GATTTCTCAT CCTCGGGATC TGGGGCTGCA TCGTATACAG CGAATACAGG AGAAACAGAG
    GTGGGCTTTG TTCCAACTTT TGGACCTTGT TATCTGAACC TTTATGGAAG CCCCCGAGAG
    TACACGGGAT TCCCAGATCC CTATGATGAG CTAAATTCTG GAAAGGGGGA AGGAGTTGCC
    TACAGAGGCA GAATCTTGGT TGAATTAAGC ACTTTACTTG AAAAGACACC ACCAGATAAA
    AAGCTTGAGT CCATTTCGCA TGATGACCTG CTGGTTGTTG AGAAATATCA GCGAAGGCGG
    AAGTACAGCC TGTCTGCTGT GTTCCATTCA GCCACCATGT TGCAAGACAT TGGCGAGGCC
    ATTCAGTTTG AAGTCAGCAT CGGGAACTAC GGCAACAAGT TTGACACGAC TTGTAAGCCT
    CTGGCATCAA CCACTCAGTA TAGCCGTGCT GTATTTGATG GAAACTACTA TTACTACTTG
    CCTTGGGCCC ACACGAAGCC AGTTGTTACA CTGACTTCCT ACTGGGAGGA TATTAGTCAT
    CGCCTGGATG CTGTGAACAC GCTCCTAGTC ATGGCAGAAA GGCTGCAATC AAACATAGAG
    GCTCTAAAAG CAGGGATACA AGGTAAAATT CCTTCAAACG AACTGGCAAA AATGTGGCAT
    AAGCTCATGG ATGAAGTTAT CGAAGACACA AGATACACGT TACCTCTCAC AGAAGGAAAA
    GCTAATGTCA CGGTTCTCGA TACTCAGATC CGAAAGTTGC GATCCAGAGC TCTCTCTCAG
    ATACATGAGG CAGCTGTGAG GATGCGGTCT GAGGTCCCAG ACGTCAAGTC CACATTGGTG
    GAAATTGAGG ACTGGCTCGA TAAATTAATG CAGCTGACTG AGGAGCCTCA GAACAGCATG
    CCTGACATCA TCATCTGGAT GATCCGGGGA GAGAAGAGAT TGGCCTACGC ACGCATTCCC
    GCGCATCAGG TTTTATACTC CACCAGCGGC GGGGATGCGT CTGGGAAGTA CTGTGGGAAA
    ACCCAGACCA TCTTTCTGAA GTATCCACAA GAAAAAAACA ACGGACCAAA GGTGCCCGTG
    GAGTTGCGTG TGAACATCTG GCTGGGCTTA AGTGCTGTTG AGAAGAAGTT CAATAGCTTC
    GCAGAAGGAA CATTCACCGT CTTTGCTGAA ATGTATGAAA ATCAAGCTCT CATGTTTGGA
    AAATGGGGCA CTTCCGGATT AGTGGGTCGT CATAAATTTT CCGATGTCAC AGGAAAAATA
    AAACTCAAGA GGGAATTCTT TTTGCCTCCA AAAGGCTGGG AATGGGAAGG AGACTGGATA
    GTTGATCCTG AAAGAAGCTT GCTGACCGAG GCGGATGCGG GTCACACAGA GTTCACAGAT
    GAAGTGTACC AGAACGAGAG TCGCTACCCC GGGGGCGAGT GGAAGCCGGC GGAGGACACC
    TACACCGATG CGAACGGCGA TAAAGCAGCA TCACCAAGCG AGCTCACTTG CCCTCCAGGC
    TGGGAATGGG AGGATGATGC ATGGGTCTAT GACATTAATC GTGCAGTGGA TGAGAGAGGC
    TGGGAATATG GAATTACCAT TCCTCCTGAT AATAAGCCCA AATCCTGGGT TGCAGCAGAA
    AAAATGTATC ATACTCATAG ACGCCGAAGG CTGGTCCGAA AACGCAAGAA AGATTTAACA
    CAATCCACTT CGAGTACCGC AAGGGCCATG GCAGAATTTG AAGACCGGGA GGGCTGGGAA
    TACGCTTCTC TAATTGGCTG GAAATTTCAC TGGAAACAGC GTAGTTCAGA TACGTTTCGC
    CGCAGACGCT GGAGGAGAAA AATGGCTCCT TCAGAAACAC ATGGTGCAGC TGCCATCTTT
    AAACTTGAAG GGGCCCTTGG CGCAGACACT ACGGACGATG GAGAGGAAAA GAGCGCAGAA
    AAGCAGAAGC ACAGCGCCAC CACTGTATTT GGAGCAAACA CTCCCATTGT TTCCTGTAAT
    TTTGACAGAG TCTACATTTA CCACCTGCGC TGCTACATCT ATCAAGCCAG GAACCTCATG
    GCTCTAGACA AGGATAGCTT TTCAGATCCA TATGCTCATG TCTCCTTCCT TCATCGAAGT
    AAAACCACCG AGATCATCCA CTCAACTCTG AATCCGACAT GGGACCAAAC GATTATATTC
    GATGAAATTG AAATTTATGG GGAACCCCAA ACCATCCTAC AGAACCCACC CAAGGTTATC
    ATCGAACTTT TTGACAATGA CCAAGTGGGC AAAGATGAAT TTTTAGGACG AAGCATGTGC
    TCTCCTCTGG TGAAACTAGA CTCAGAAATG GACATCACTC CCAAACTTCT CTGGTACCCA
    GTGATGAATG GAGACAAAAC CTGTGGGGAT GTCCTTGTAA CTGCAGAGTT GATTCTGAGG
    CACAAGGATG GCTCCAACCT TCCCATTCTT CCCTCGCAAA GGGCACCAAA TCTGTACATG
    GTCCCCCAGG GCATCAGGCC TGTGGTTCAG CTCACTGCTA TTGAGATTCT AGCTTGGGGC
    TTAAGAAATA TGAAGAATTA CCAGATGGCT TCTGTCACCT CCCCCAGTCT CATTGTGGAG
    TGTGGAGGGG AAAGGGTCGA GTCAGTGGTG ATCAAAAACC TGAAGAAGAC ACCCAACTTC
    CCAAGTTCTG TTCTCTTCAT GAGAGTGTTC TTGCCCAAGG AGGAACTGTA CATGCCTCCA
    CTGGTGATCA AGGTCATCGA CCACCGGCAG TTCGGGCGCA AGCCCGTTGT GGGCCAGTGC
    ACCATTGATC GCCTGGACCG CTTTCGCTGT GACCCTTATG CAGGAAAGGA GGACATCGTG
    CCACAGCTAA AAGCCTCCTT TTTGTCTACC CCACCCCGCC GGGACATTGT TATTGAAATA
    GAGGACACCA AGCCATTACT AGCTTCAAAG TGCTTAAGCA GTATGTCAAC AGCACTCAGC
    AAAATGGCTT CTCCAAGGAC AGTGCATCTA ACAGAAAAGG AGGAAGAAAT TGTTGACTGG
    TGGAGTAAAT TTTATGCTTC CACAGGGGAA CATGAAAAAT GTGGACAATA TATTCAGAAA
    GGTTATTCCA AACTCAAGAT ATATGATTGT GAACTAGAGG ATGTAGAGGA ATTCGAGGGC
    CTGACGGACT TCTCAGATAC TTTCAAGCTG TTTCGAGGCA AATCAGATGA GAATGAAGAC
    CCTTCTGTGG TAGGGGAGTT TAAGGGCTCC TTCCGAATCT ACCCACTGCC GGATGACCCC
    AGCGTGCCGG CCCCTCCCCG GCAGTTTCGG GAGTTACCTG ACAGCGTCCC GCAGGAATGC
    ACGGTTAGGA TTTACATTGT TCGAGGCTTA GAGCTCCAGC CCCAGGACAA CAATGGCCTG
    TGTGACCCTT ACATAAAAAT AACACTGGGC AAAAAAGTAA TTGAAGACCG AGACAACTAC
    ATCCCCAACA CTCTCAACCC GGTGTTCGGC AGAATGTATG AACTGAGCTG CTACTTACCT
    CAAGAAAAGG ACCTGAAAAT TTCTGTCTAC GATTATGACA CCTTTACCCG TGATGAAAAA
    GTAGGAGAAA CGATCATTGA CCTAGAAAAC CGATTCCTTT CCCGGTTTGG GTCCCACTGT
    GGCATTCCTG AGCAGTACTG CGTTTCTGGA GTAAACACCT GGCGAGATCA ATTGAAACCA
    ACACAGTTGC TTCAAAATGT GGCCAGATTC AAAGGCTTCC CACCGCCCGT TCTTTCTGAA
    GATGGAAGTA GCATCAGATA TGGAGGAAAA GACTACGGCC TAGATGAATT TGAAGCAAAC
    AAAATCCTGC ACCAGCACCT TGGGCCCCCT GAGGAGCGCC TTGCCCTCTA CATCCTCAGG
    ACTCAGGGGC TGGTTCCTGA ACACGTGGAA ACGAGGACTT TGCACAGCAG CTTCCAGCCT
    AACATCTCCC AGGGAAGACT TCAGATGTGG GTGGATGTTT TCCCCAAGAA TTTGGGGCCA
    CCAGGCCCTC CTTTCAACAT CACACCCCGG AAAGCCAAGA AATACTACCT GCGTGTGATT
    ATCTGGAACA CCAAGGATGT TGTCCTGGAC GAGAAAAGCA TCACAGGAGA GGAAATGAGC
    GACATCTATG TGAAAGGCTG GATTCCTGGC AATGAAGAAA ACAAACAGAA AACAGATGTC
    CATTACAGGT CCTTGGATGG CGTAGGGAAC TTTAACTGGA GATTCGTCTT CCCTTTTGAC
    TACCTCCCAG CTGAACAGCT CTGTCTCGTT GCTAAAAAAG AGCATTTCTG GAGTATTGAC
    CAAACTGAAT TTCGGGTCCC ACCCAGACTG GTCATTCAGA TATGGGACAA TGACAAGTTT
    TCTCTGGATG ACTACTTGGG TTTCCTGGAA CTTGATTTGC ATCATACCAT CATTCCAGCA
    AAGTCACCAG AGAAATGCAG TTTGAACATG ATTCCAGACC TCAAAGCCAT GAACCCCCTG
    AAAGTGAAAA CTGCCTCTCT CTTTGAGCAG AAGTCCATGA AAGGATGGTG GCCTTGCTAC
    ACAGAGAAAG ATGGCTCCCG TGTGATGGCT GGGAAAGTGG AGATGACGCT GGAAGTCCTC
    AATGAGAAGG AGGCCGACGA GAGGCCAGCT GGGAAGGGAC GGGATGAACC CAACATGAAC
    CCCAAGCTGG ACCCACCAAA CCGACCAGAA ACCTCCTTCC TCTGGTTCAC CAACCCGTGC
    AAGACCATGA AGTTCATCGT GTGGCGCCGA TTTAAGTGGG TCATCATTGC CCTGCTGATC
    CTGCTGATCC TGCTGTTCTT TGTGGCTGTG TTTCTGTATT CCTTACCGAA CTATTTGTCA
    ATGAAGATTG TGAAACCAAA TGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216187
    Clone ID Related Accession (Same CDS sequence) NM_001193212.2
    Accession Version NM_001193212.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product myoferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02058830.1, DAAA02058829.1 and DAAA02058828.1. On Jun 30, 2017 this sequence version replaced NM_001193212.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180141.1
    CDS90..6275
    Translation

    Target ORF information:

    RefSeq Version NM_001193212.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193212.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239152
    Clone ID Related Accession (Same CDS sequence) NM_001193212.3
    Accession Version NM_001193212.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-17
    Organism Bos taurus(cattle)
    Product myoferlin
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000026.1, EV684499.1 and CB419566.1. On Jun 18, 2018 this sequence version replaced NM_001193212.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGACCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGTGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTCT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180141.2
    CDS90..6275
    Translation

    Target ORF information:

    RefSeq Version NM_001193212.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193212.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGACCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGTGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAAGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCTG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTCT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACCTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230831
    Clone ID Related Accession (Same CDS sequence) XM_024985587.1
    Accession Version XM_024985587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGAGGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC
    CCCGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG
    GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG
    TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC
    CGGAAAGCCA AGAAATACTA CTTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG
    GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT
    GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG
    AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC
    GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA
    CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG
    GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC
    ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG
    CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG
    GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA
    GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA
    GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC
    CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT
    GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841355.1
    CDS248..6487
    Translation

    Target ORF information:

    RefSeq Version XM_024985587.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGAGGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC
    CCCGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG
    GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG
    TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC
    CGGAAAGCCA AGAAATACTA CTTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG
    GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT
    GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG
    AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC
    GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA
    CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG
    GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC
    ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG
    CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG
    GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA
    GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA
    GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC
    CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT
    GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230832
    Clone ID Related Accession (Same CDS sequence) XM_024985588.1
    Accession Version XM_024985588.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG
    GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT
    AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT
    TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG
    GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT
    GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG
    CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT
    AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC
    CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC
    AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA
    AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA
    GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT
    CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG
    TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA
    AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAGGC AAACAAAATC
    CTGCACCAGC ACCTTGGGCC CCCCGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG
    GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC
    TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC
    CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACTTGCGTGT GATTATCTGG
    AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC
    TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC
    AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC
    CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT
    GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG
    GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA
    CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG
    AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG
    AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG
    AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG
    CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC
    ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG
    ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG
    ATTGTGAAAC CAAATGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841356.1
    CDS249..6449
    Translation

    Target ORF information:

    RefSeq Version XM_024985588.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985588.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG
    GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT
    AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT
    TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG
    GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT
    GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG
    CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT
    AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC
    CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC
    AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA
    AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA
    GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT
    CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG
    TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA
    AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAGGC AAACAAAATC
    CTGCACCAGC ACCTTGGGCC CCCCGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG
    GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC
    TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC
    CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACTTGCGTGT GATTATCTGG
    AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC
    TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC
    AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC
    CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT
    GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG
    GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA
    CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG
    AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG
    AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG
    AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG
    CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC
    ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG
    ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG
    ATTGTGAAAC CAAATGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230833
    Clone ID Related Accession (Same CDS sequence) XM_024985589.1
    Accession Version XM_024985589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA
    GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT
    GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC
    AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG
    GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG
    TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA
    GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA
    CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG
    TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT
    GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA
    GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT
    TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC
    AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA
    GGAAAAGACT ACGGCCTAGA TGAATTTGAG GCAAACAAAA TCCTGCACCA GCACCTTGGG
    CCCCCCGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC
    GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG
    ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA
    CCCCGGAAAG CCAAGAAATA CTACTTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC
    CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT
    CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA
    GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT
    CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC
    AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC
    CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG
    AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT
    GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG
    ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG
    CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA
    CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG
    CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG
    GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841357.1
    CDS248..6430
    Translation

    Target ORF information:

    RefSeq Version XM_024985589.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA
    GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT
    GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC
    AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG
    GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG
    TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA
    GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA
    CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG
    TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT
    GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA
    GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT
    TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC
    AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA
    GGAAAAGACT ACGGCCTAGA TGAATTTGAG GCAAACAAAA TCCTGCACCA GCACCTTGGG
    CCCCCCGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC
    GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG
    ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA
    CCCCGGAAAG CCAAGAAATA CTACTTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC
    CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT
    CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA
    GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT
    CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC
    AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC
    CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG
    AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT
    GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG
    ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG
    CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA
    CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG
    CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG
    GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa230834
    Clone ID Related Accession (Same CDS sequence) XM_024985591.1
    Accession Version XM_024985591.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product myoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037353.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG
    AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT
    TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG
    ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT
    TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC
    GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG
    GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT
    GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC
    CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA
    GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA
    GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC
    ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA
    CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT
    GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA GGCAAACAAA
    ATCCTGCACC AGCACCTTGG GCCCCCCGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT
    CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC
    ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA
    GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACTTGCG TGTGATTATC
    TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC
    ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT
    TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC
    CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA
    ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT
    CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG
    TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA
    GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA
    GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT
    GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC
    AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG
    ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG
    CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG
    AAGATTGTGA AACCAAATGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841359.1
    CDS248..6391
    Translation

    Target ORF information:

    RefSeq Version XM_024985591.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024985591.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG ACCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG TGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG
    AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT
    TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG
    ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT
    TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC
    GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG
    GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT
    GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC
    CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA
    GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA
    GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC
    ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA
    CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT
    GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA GGCAAACAAA
    ATCCTGCACC AGCACCTTGG GCCCCCCGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT
    CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC
    ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA
    GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACTTGCG TGTGATTATC
    TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC
    ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT
    TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC
    CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA
    ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT
    CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG
    TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA
    GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA
    GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT
    GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC
    AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG
    ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG
    CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG
    AAGATTGTGA AACCAAATGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40769
    Clone ID Related Accession (Same CDS sequence) NM_001193212.1
    Accession Version NM_001193212.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product myoferlin
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03042346.1, AAFC03125080.1, AAFC03073515.1, AAFC02005930.1, AAFC02115478.1, AAFC03073516.1 and AAFC03117136.1. On Jul 17, 2010 this sequence version replaced XM_873525.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAGGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCCG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACTTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001180141.1
    CDS90..6275
    Translation

    Target ORF information:

    RefSeq Version NM_001193212.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus myoferlin (MYOF), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001193212.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGGAGACAA AGAGGAAGAT GGAGGTGATG AGGACATGTT GGACAGTACG
    GTCAGGGGCC CAGGGCCCAG GGGGCCAGGA GGGACCGTGC CGGAAGCTCA CCTTGCCCGG
    AGGCTCACGA AAGCGAAGAG CAGCCGCCGC ATGCTGTCTA ACAAGCCACA GGACTTCCAG
    ATCCGGGTCC GAGTGATCGA GGGCCGGCAG TTGAGTGGCA ACAACATCAA GCCTGTGGTC
    AAAGTCCACA TCTGTGGCCA GACGCATCGA ACAAGAATCA AGCGAGGGAA CAATCCATTT
    TTTGATGAGT TATTTTTCTA CAATGTCCAC CTGACTCCTT CTGAACTGAT GGATGAGATC
    ATCAGCATTC GGGTTTACAA TTCCCATTCT CTGCGGGCAG ATTCTCTGAT GGGGGAGTTT
    AAGATCGATG TTGGATTTGT TTATGATGAA CCTGGTCATG CTGTCATGAG GAAATGGCTT
    CTCCTCAATG ACCCTGAAGA TACCAGCTCG GGTGCTAAAG GCTACCTGAA AGTCAGCATG
    TTCGTCCTGG GAACTGGAGA CGAGCCTCCT CCTGAGAAAC AAGATCGTGA TAATGACAGT
    GATGACGTGG AAAGTAACTT GTTACTGCCT GCTGGCATTG CCCTGCGGTG GGTGACCTTC
    CTGCTGAAGA TCTACAGAGC GGAGGACATC CCCCAGATGG ATGATGCCTT CTCACAGACA
    GTGAAGGAAA TATTTGGAGG CGTCGCTGAT AAGAAGAACC TAGTGGACCC TTTTGTAGAA
    GTTTCCTTTG CCGGAAAAAA GGTTTGCACG AACATAATTG AGAAAAATGC AAACCCTGAG
    TGGAATCAAG TCGTTAATCT TCAGATCAAG TTTCCTTCAG TGTGTGAAAA AATAAAACTG
    ACAATATATG ACTGGGACCG TCTTACCAAA AATGATGTTG TTGGAACAAC GTATCTGCAC
    CTCTCTAAAA TTGCTGCCTC TGGTGGGGAA GTGGAAGATT TCTCATCCTC GGGATCTGGG
    GCTGCATCGT ATACAGCGAA TACAGGAGAA ACAGAGGTGG GCTTTGTTCC AACTTTTGGA
    CCTTGTTATC TGAACCTTTA TGGAAGCCCC CGAGAGTACA CGGGATTCCC AGATCCCTAT
    GATGAGCTAA ATTCTGGAAA GGGGGAAGGA GTTGCCTACA GAGGCAGAAT CTTGGTTGAA
    TTAAGCACTT TACTTGAAAA GACACCACCA GATAAAAAGC TTGAGTCCAT TTCGCATGAT
    GACCTGCTGG TTGTTGAGAA ATATCAGCGA AGGCGGAAGT ACAGCCTGTC TGCTGTGTTC
    CATTCAGCCA CCATGTTGCA AGACATTGGC GAGGCCATTC AGTTTGAAGT CAGCATCGGG
    AACTACGGCA ACAAGTTTGA CACGACTTGT AAGCCTCTGG CATCAACCAC TCAGTATAGC
    CGTGCTGTAT TTGATGGAAA CTACTATTAC TACTTGCCTT GGGCCCACAC GAAGCCAGTT
    GTTACACTGA CTTCCTACTG GGAGGATATT AGTCATCGCC TGGATGCTGT GAACACGCTC
    CTAGTCATGG CAGAAAGGCT GCAATCAAAC ATAGAGGCTC TAAAAGCAGG GATACAAGGT
    AAAATTCCTT CAAACGAACT GGCAAAAATG TGGCATAAGC TCATGGATGA AGTTATCGAA
    GACACAAGAT ACACGTTACC TCTCACAGAA GGAAAAGCTA ATGTCACGGT TCTCGATACT
    CAGATCCGAA AGTTGCGATC CAGAGCTCTC TCTCAGATAC ATGAGGCAGC TGTGAGGATG
    CGGTCTGAGG TCCCAGACGT CAAGTCCACA TTGGTGGAAA TTGAGGACTG GCTCGATAAA
    TTAATGCAGC TGACTGAGGA GCCTCAGAAC AGCATGCCTG ACATCATCAT CTGGATGATC
    CGGGGAGAGA AGAGATTGGC CTACGCACGC ATTCCCGCGC ATCAGGTTTT ATACTCCACC
    AGCGGCGGGG ATGCGTCTGG GAAGTACTGT GGGAAAACCC AGACCATCTT TCTGAAGTAT
    CCACAAGAAA AAAACAACGG ACCAAAGGTG CCCGTGGAGT TGCGTGTGAA CATCTGGCTG
    GGCTTAAGTG CTGTTGAGAA GAAGTTCAAT AGCTTCGCAG AAGGAACATT CACCGTCTTT
    GCTGAAATGT ATGAAAATCA AGCTCTCATG TTTGGAAAAT GGGGCACTTC CGGATTAGTG
    GGTCGTCATA AATTTTCCGA TGTCACAGGA AAAATAAAAC TCAAGAGGGA ATTCTTTTTG
    CCTCCAAAAG GCTGGGAATG GGAAGGAGAC TGGATAGTTG ATCCTGAAAG AAGCTTGCTG
    ACCGAGGCGG ATGCGGGTCA CACAGAGTTC ACAGATGAAG TGTACCAGAA CGAGAGTCGC
    TACCCCGGGG GCGAGTGGAA GCCGGCGGAG GACACCTACA CCGATGCGAA CGGCGATAAA
    GCAGCATCAC CAAGCGAGCT CACTTGCCCT CCAGGCTGGG AATGGGAGGA TGATGCATGG
    GTCTATGACA TTAATCGTGC AGTGGATGAG AGAGGCTGGG AATATGGAAT TACCATTCCT
    CCTGATAATA AGCCCAAATC CTGGGTTGCA GCAGAAAAAA TGTATCATAC TCATAGACGC
    CGAAGGCTGG TCCGAAAACG CAAGAAAGAT TTAACACAAT CCACTTCGAG TACCGCAAGG
    GCCATGGCAG AATTTGAAGA CCGGGAGGGC TGGGAATACG CTTCTCTAAT TGGCTGGAAA
    TTTCACTGGA AACAGCGTAG TTCAGATACG TTTCGCCGCA GACGCTGGAG GAGAAAAATG
    GCTCCTTCAG AAACACATGG TGCAGCTGCC ATCTTTAAAC TTGAAGGGGC CCTTGGCGCA
    GACACTACGG ACGATGGAGA GGAAAAGAGC GCAGAAAAGC AGAAGCACAG CGCCACCACT
    GTATTTGGAG CAAACACTCC CATTGTTTCC TGTAATTTTG ACAGAGTCTA CATTTACCAC
    CTGCGCTGCT ACATCTATCA AGCCAGGAAC CTCATGGCTC TAGACAAGGA TAGCTTTTCA
    GATCCATATG CTCATGTCTC CTTCCTTCAT CGAAGTAAAA CCACCGAGAT CATCCACTCA
    ACTCTGAATC CGACATGGGA CCAAACGATT ATATTCGATG AAATTGAAAT TTATGGGGAA
    CCCCAAACCA TCCTACAGAA CCCACCCAAG GTTATCATCG AACTTTTTGA CAATGACCAA
    GTGGGCAAAG ATGAATTTTT AGGACGAAGC ATGTGCTCTC CTCTGGTGAA ACTAGACTCA
    GAAATGGACA TCACTCCCAA ACTTCTCTGG TACCCAGTGA TGAATGGAGA CAAAACCTGT
    GGGGATGTCC TTGTAACTGC AGAGTTGATT CTGAGGCACA AGGATGGCTC CAACCTTCCC
    ATTCTTCCCT CGCAAAGGGC ACCAAATCTG TACATGGTCC CCCAGGGCAT CAGGCCTGTG
    GTTCAGCTCA CTGCTATTGA GATTCTAGCT TGGGGCTTAA GAAATATGAA GAATTACCAG
    ATGGCTTCTG TCACCTCCCC CAGTCTCATT GTGGAGTGTG GAGGGGAAAG GGTCGAGTCA
    GTGGTGATCA AAAACCTGAA GAAGACACCC AACTTCCCAA GTTCTGTTCT CTTCATGAGA
    GTGTTCTTGC CCAAGGAGGA ACTGTACATG CCTCCACTGG TGATCAAGGT CATCGACCAC
    CGGCAGTTCG GGCGCAAGCC CGTTGTGGGC CAGTGCACCA TTGATCGCCT GGACCGCTTT
    CGCTGTGACC CTTATGCAGG AAAGGAGGAC ATCGTGCCAC AGCTAAAAGC CTCCTTTTTG
    TCTACCCCAC CCCGCCGGGA CATTGTTATT GAAATAGAGG ACACCAAGCC ATTACTAGCT
    TCAAAGCTAA CAGAAAAGGA GGAAGAAATT GTTGACTGGT GGAGTAAATT TTATGCTTCC
    ACAGGGGAAC ATGAAAAATG TGGACAATAT ATTCAGAAAG GTTATTCCAA ACTCAAGATA
    TATGATTGTG AACTAGAGGA TGTAGAGGAA TTCGAGGGCC TGACGGACTT CTCAGATACT
    TTCAAGCTGT TTCGAGGCAA ATCAGATGAG AATGAAGACC CTTCTGTGGT AGGGGAGTTT
    AAGGGCTCCT TCCGAATCTA CCCACTGCCG GATGACCCCA GCGTGCCGGC CCCTCCCCGG
    CAGTTTCGGG AGTTACCTGA CAGCGTCCCG CAGGAATGCA CGGTTAGGAT TTACATTGTT
    CGAGGCTTAG AGCTCCAGCC CCAGGACAAC AATGGCCTGT GTGACCCTTA CATAAAAATA
    ACACTGGGCA AAAAAGTAAT TGAAGACCGA GACAACTACA TCCCCAACAC TCTCAACCCG
    GTGTTCGGCA GAATGTATGA ACTGAGCTGC TACTTACCTC AAGAAAAGGA CCTGAAAATT
    TCTGTCTACG ATTATGACAC CTTTACCCGT GATGAAAAAG TAGGAGAAAC GATCATTGAC
    CTAGAAAACC GATTCCTTTC CCGGTTTGGG TCCCACTGTG GCATTCCTGA GCAGTACTGC
    GTTTCTGGAG TAAACACCTG GCGAGATCAA TTGAAACCAA CACAGTTGCT TCAAAATGTG
    GCCAGATTCA AAGGCTTCCC ACCGCCCGTT CTTTCTGAAG ATGGAAGTAG CATCAGATAT
    GGAGGAAAAG ACTACGGCCT AGATGAATTT GAGGCAAACA AAATCCTGCA CCAGCACCTT
    GGGCCCCCCG AGGAGCGCCT TGCCCTCTAC ATCCTCAGGA CTCAGGGGCT GGTTCCTGAA
    CACGTGGAAA CGAGGACTTT GCACAGCAGC TTCCAGCCTA ACATCTCCCA GGGAAGACTT
    CAGATGTGGG TGGATGTTTT CCCCAAGAAT TTGGGGCCAC CAGGCCCTCC TTTCAACATC
    ACACCCCGGA AAGCCAAGAA ATACTACTTG CGTGTGATTA TCTGGAACAC CAAGGATGTT
    GTCCTGGACG AGAAAAGCAT CACAGGAGAG GAAATGAGCG ACATCTATGT GAAAGGCTGG
    ATTCCTGGCA ATGAAGAAAA CAAACAGAAA ACAGATGTCC ATTACAGGTC CTTGGATGGC
    GTAGGGAACT TTAACTGGAG ATTCGTCTTC CCTTTTGACT ACCTCCCAGC TGAACAGCTC
    TGTCTCGTTG CTAAAAAAGA GCATTTCTGG AGTATTGACC AAACTGAATT TCGGGTCCCA
    CCCAGACTGG TCATTCAGAT ATGGGACAAT GACAAGTTTT CTCTGGATGA CTACTTGGGT
    TTCCTGGAAC TTGATTTGCA TCATACCATC ATTCCAGCAA AGTCACCAGA GAAATGCAGT
    TTGAACATGA TTCCAGACCT CAAAGCCATG AACCCCCTGA AAGTGAAAAC TGCCTCTCTC
    TTTGAGCAGA AGTCCATGAA AGGATGGTGG CCTTGCTACA CAGAGAAAGA TGGCTCCCGT
    GTGATGGCTG GGAAAGTGGA GATGACGCTG GAAGTCCTCA ATGAGAAGGA GGCCGACGAG
    AGGCCAGCTG GGAAGGGACG GGATGAACCC AACATGAACC CCAAGCTGGA CCCACCAAAC
    CGACCAGAAA CCTCCTTCCT CTGGTTCACC AACCCGTGCA AGACCATGAA GTTCATCGTG
    TGGCGCCGAT TTAAGTGGGT CATCATTGCC CTGCTGATCC TGCTGATCCT GCTGTTCTTT
    GTGGCTGTGT TTCTGTATTC CTTACCGAAC TATTTGTCAA TGAAGATTGT GAAACCAAAT
    GCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa33555
    Clone ID Related Accession (Same CDS sequence) XM_005225521.3
    Accession Version XM_005225521.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005225521.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGAAGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC
    CCTGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG
    GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG
    TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC
    CGGAAAGCCA AGAAATACTA CCTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG
    GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT
    GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG
    AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC
    GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA
    CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG
    GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC
    ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG
    CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG
    GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA
    GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA
    GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC
    CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT
    GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005225578.1
    CDS240..6479
    Translation

    Target ORF information:

    RefSeq Version XM_005225521.3
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005225521.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGTGCTTAA GCAGTATGTC AACAGCACTC AGCAAAATGG CTTCTCCAAG GACAGTGCAT
    CTAACAGAAA AGGAGGAAGA AATTGTTGAC TGGTGGAGTA AATTTTATGC TTCCACAGGG
    GAACATGAAA AATGTGGACA ATATATTCAG AAAGGTTATT CCAAACTCAA GATATATGAT
    TGTGAACTAG AGGATGTAGA GGAATTCGAG GGCCTGACGG ACTTCTCAGA TACTTTCAAG
    CTGTTTCGAG GCAAATCAGA TGAGAATGAA GACCCTTCTG TGGTAGGGGA GTTTAAGGGC
    TCCTTCCGAA TCTACCCACT GCCGGATGAC CCCAGCGTGC CGGCCCCTCC CCGGCAGTTT
    CGGGAGTTAC CTGACAGCGT CCCGCAGGAA TGCACGGTTA GGATTTACAT TGTTCGAGGC
    TTAGAGCTCC AGCCCCAGGA CAACAATGGC CTGTGTGACC CTTACATAAA AATAACACTG
    GGCAAAAAAG TAATTGAAGA CCGAGACAAC TACATCCCCA ACACTCTCAA CCCGGTGTTC
    GGCAGAATGT ATGAACTGAG CTGCTACTTA CCTCAAGAAA AGGACCTGAA AATTTCTGTC
    TACGATTATG ACACCTTTAC CCGTGATGAA AAAGTAGGAG AAACGATCAT TGACCTAGAA
    AACCGATTCC TTTCCCGGTT TGGGTCCCAC TGTGGCATTC CTGAGCAGTA CTGCGTTTCT
    GGAGTAAACA CCTGGCGAGA TCAATTGAAA CCAACACAGT TGCTTCAAAA TGTGGCCAGA
    TTCAAAGGCT TCCCACCGCC CGTTCTTTCT GAAGATGGAA GTAGCATCAG ATATGGAGGA
    AAAGACTACG GCCTAGATGA ATTTGAAGCA AACAAAATCC TGCACCAGCA CCTTGGGCCC
    CCTGAGGAGC GCCTTGCCCT CTACATCCTC AGGACTCAGG GGCTGGTTCC TGAACACGTG
    GAAACGAGGA CTTTGCACAG CAGCTTCCAG CCTAACATCT CCCAGGGAAG ACTTCAGATG
    TGGGTGGATG TTTTCCCCAA GAATTTGGGG CCACCAGGCC CTCCTTTCAA CATCACACCC
    CGGAAAGCCA AGAAATACTA CCTGCGTGTG ATTATCTGGA ACACCAAGGA TGTTGTCCTG
    GACGAGAAAA GCATCACAGG AGAGGAAATG AGCGACATCT ATGTGAAAGG CTGGATTCCT
    GGCAATGAAG AAAACAAACA GAAAACAGAT GTCCATTACA GGTCCTTGGA TGGCGTAGGG
    AACTTTAACT GGAGATTCGT CTTCCCTTTT GACTACCTCC CAGCTGAACA GCTCTGTCTC
    GTTGCTAAAA AAGAGCATTT CTGGAGTATT GACCAAACTG AATTTCGGGT CCCACCCAGA
    CTGGTCATTC AGATATGGGA CAATGACAAG TTTTCTCTGG ATGACTACTT GGGTTTCCTG
    GAACTTGATT TGCATCATAC CATCATTCCA GCAAAGTCAC CAGAGAAATG CAGTTTGAAC
    ATGATTCCAG ACCTCAAAGC CATGAACCCC CTGAAAGTGA AAACTGCCTC TCTCTTTGAG
    CAGAAGTCCA TGAAAGGATG GTGGCCTTGC TACACAGAGA AAGATGGCTC CCGTGTGATG
    GCTGGGAAAG TGGAGATGAC GCTGGAAGTC CTCAATGAGA AGGAGGCCGA CGAGAGGCCA
    GCTGGGAAGG GACGGGATGA ACCCAACATG AACCCCAAGC TGGACCCACC AAACCGACCA
    GAAACCTCCT TCCTCTGGTT CACCAACCCG TGCAAGACCA TGAAGTTCAT CGTGTGGCGC
    CGATTTAAGT GGGTCATCAT TGCCCTGCTG ATCCTGCTGA TCCTGCTGTT CTTTGTGGCT
    GTGTTTCTGT ATTCCTTACC GAACTATTTG TCAATGAAGA TTGTGAAACC AAATGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48674
    Clone ID Related Accession (Same CDS sequence) XM_010819883.2
    Accession Version XM_010819883.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6201bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819883.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG
    GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT
    AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT
    TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG
    GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT
    GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG
    CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT
    AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC
    CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC
    AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA
    AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA
    GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT
    CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG
    TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA
    AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAAGC AAACAAAATC
    CTGCACCAGC ACCTTGGGCC CCCTGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG
    GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC
    TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC
    CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACCTGCGTGT GATTATCTGG
    AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC
    TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC
    AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC
    CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT
    GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG
    GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA
    CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG
    AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG
    AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG
    AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG
    CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC
    ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG
    ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG
    ATTGTGAAAC CAAATGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818185.1
    CDS240..6440
    Translation

    Target ORF information:

    RefSeq Version XM_010819883.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819883.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGTGCTTA AGCAGTATGT CAACAGCACT CAGCAAAATG
    GCTTCTCCAA GGACAGTGCA TCTAACAGAA AAGGAGGAAG AAATTGTTGA CTGGTGGAGT
    AAATTTTATG CTTCCACAGG GGAACATGAA AAATGTGGAC AATATATTCA GAAAGGTTAT
    TCCAAACTCA AGATATATGA TTGTGAACTA GAGGATGTAG AGGAATTCGA GGGCCTGACG
    GACTTCTCAG ATACTTTCAA GCTGTTTCGA GGCAAATCAG ATGAGAATGA AGACCCTTCT
    GTGGTAGGGG AGTTTAAGGG CTCCTTCCGA ATCTACCCAC TGCCGGATGA CCCCAGCGTG
    CCGGCCCCTC CCCGGCAGTT TCGGGAGTTA CCTGACAGCG TCCCGCAGGA ATGCACGGTT
    AGGATTTACA TTGTTCGAGG CTTAGAGCTC CAGCCCCAGG ACAACAATGG CCTGTGTGAC
    CCTTACATAA AAATAACACT GGGCAAAAAA GTAATTGAAG ACCGAGACAA CTACATCCCC
    AACACTCTCA ACCCGGTGTT CGGCAGAATG TATGAACTGA GCTGCTACTT ACCTCAAGAA
    AAGGACCTGA AAATTTCTGT CTACGATTAT GACACCTTTA CCCGTGATGA AAAAGTAGGA
    GAAACGATCA TTGACCTAGA AAACCGATTC CTTTCCCGGT TTGGGTCCCA CTGTGGCATT
    CCTGAGCAGT ACTGCGTTTC TGGAGTAAAC ACCTGGCGAG ATCAATTGAA ACCAACACAG
    TTGCTTCAAA ATGTGGCCAG ATTCAAAGGC TTCCCACCGC CCGTTCTTTC TGAAGATGGA
    AGTAGCATCA GATATGGAGG AAAAGACTAC GGCCTAGATG AATTTGAAGC AAACAAAATC
    CTGCACCAGC ACCTTGGGCC CCCTGAGGAG CGCCTTGCCC TCTACATCCT CAGGACTCAG
    GGGCTGGTTC CTGAACACGT GGAAACGAGG ACTTTGCACA GCAGCTTCCA GCCTAACATC
    TCCCAGGGAA GACTTCAGAT GTGGGTGGAT GTTTTCCCCA AGAATTTGGG GCCACCAGGC
    CCTCCTTTCA ACATCACACC CCGGAAAGCC AAGAAATACT ACCTGCGTGT GATTATCTGG
    AACACCAAGG ATGTTGTCCT GGACGAGAAA AGCATCACAG GAGAGGAAAT GAGCGACATC
    TATGTGAAAG GCTGGATTCC TGGCAATGAA GAAAACAAAC AGAAAACAGA TGTCCATTAC
    AGGTCCTTGG ATGGCGTAGG GAACTTTAAC TGGAGATTCG TCTTCCCTTT TGACTACCTC
    CCAGCTGAAC AGCTCTGTCT CGTTGCTAAA AAAGAGCATT TCTGGAGTAT TGACCAAACT
    GAATTTCGGG TCCCACCCAG ACTGGTCATT CAGATATGGG ACAATGACAA GTTTTCTCTG
    GATGACTACT TGGGTTTCCT GGAACTTGAT TTGCATCATA CCATCATTCC AGCAAAGTCA
    CCAGAGAAAT GCAGTTTGAA CATGATTCCA GACCTCAAAG CCATGAACCC CCTGAAAGTG
    AAAACTGCCT CTCTCTTTGA GCAGAAGTCC ATGAAAGGAT GGTGGCCTTG CTACACAGAG
    AAAGATGGCT CCCGTGTGAT GGCTGGGAAA GTGGAGATGA CGCTGGAAGT CCTCAATGAG
    AAGGAGGCCG ACGAGAGGCC AGCTGGGAAG GGACGGGATG AACCCAACAT GAACCCCAAG
    CTGGACCCAC CAAACCGACC AGAAACCTCC TTCCTCTGGT TCACCAACCC GTGCAAGACC
    ATGAAGTTCA TCGTGTGGCG CCGATTTAAG TGGGTCATCA TTGCCCTGCT GATCCTGCTG
    ATCCTGCTGT TCTTTGTGGC TGTGTTTCTG TATTCCTTAC CGAACTATTT GTCAATGAAG
    ATTGTGAAAC CAAATGCATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48675
    Clone ID Related Accession (Same CDS sequence) XM_010819884.2
    Accession Version XM_010819884.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6183bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819884.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA
    GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT
    GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC
    AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG
    GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG
    TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA
    GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA
    CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG
    TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT
    GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA
    GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT
    TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC
    AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA
    GGAAAAGACT ACGGCCTAGA TGAATTTGAA GCAAACAAAA TCCTGCACCA GCACCTTGGG
    CCCCCTGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC
    GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG
    ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA
    CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC
    CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT
    CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA
    GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT
    CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC
    AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC
    CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG
    AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT
    GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG
    ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG
    CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA
    CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG
    CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG
    GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818186.1
    CDS240..6422
    Translation

    Target ORF information:

    RefSeq Version XM_010819884.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819884.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGATTTCT CATCCTCGGG ATCTGGGGCT
    GCATCGTATA CAGCGAATAC AGGAGAAACA GAGGTGGGCT TTGTTCCAAC TTTTGGACCT
    TGTTATCTGA ACCTTTATGG AAGCCCCCGA GAGTACACGG GATTCCCAGA TCCCTATGAT
    GAGCTAAATT CTGGAAAGGG GGAAGGAGTT GCCTACAGAG GCAGAATCTT GGTTGAATTA
    AGCACTTTAC TTGAAAAGAC ACCACCAGAT AAAAAGCTTG AGTCCATTTC GCATGATGAC
    CTGCTGGTTG TTGAGAAATA TCAGCGAAGG CGGAAGTACA GCCTGTCTGC TGTGTTCCAT
    TCAGCCACCA TGTTGCAAGA CATTGGCGAG GCCATTCAGT TTGAAGTCAG CATCGGGAAC
    TACGGCAACA AGTTTGACAC GACTTGTAAG CCTCTGGCAT CAACCACTCA GTATAGCCGT
    GCTGTATTTG ATGGAAACTA CTATTACTAC TTGCCTTGGG CCCACACGAA GCCAGTTGTT
    ACACTGACTT CCTACTGGGA GGATATTAGT CATCGCCTGG ATGCTGTGAA CACGCTCCTA
    GTCATGGCAG AAAGGCTGCA ATCAAACATA GAGGCTCTAA AAGCAGGGAT ACAAGGTAAA
    ATTCCTTCAA ACGAACTGGC AAAAATGTGG CATAAGCTCA TGGATGAAGT TATCGAAGAC
    ACAAGATACA CGTTACCTCT CACAGAAGGA AAAGCTAATG TCACGGTTCT CGATACTCAG
    ATCCGAAAGT TGCGATCCAG AGCTCTCTCT CAGATACATG AGGCAGCTGT GAGGATGCGG
    TCTGAGGTCC CAGACGTCAA GTCCACATTG GTGGAAATTG AGGACTGGCT CGATAAATTA
    ATGCAGCTGA CTGAGGAGCC TCAGAACAGC ATGCCTGACA TCATCATCTG GATGATCCGG
    GGAGAGAAGA GATTGGCCTA CGCACGCATT CCCGCGCATC AGGTTTTATA CTCCACCAGC
    GGCGGGGATG CGTCTGGGAA GTACTGTGGG AAAACCCAGA CCATCTTTCT GAAGTATCCA
    CAAGAAAAAA ACAACGGACC AAAGGTGCCC GTGGAGTTGC GTGTGAACAT CTGGCTGGGC
    TTAAGTGCTG TTGAGAAGAA GTTCAATAGC TTCGCAGAAG GAACATTCAC CGTCTTTGCT
    GAAATGTATG AAAATCAAGC TCTCATGTTT GGAAAATGGG GCACTTCCGG ATTAGTGGGT
    CGTCATAAAT TTTCCGATGT CACAGGAAAA ATAAAACTCA AGAGGGAATT CTTTTTGCCT
    CCAAAAGGCT GGGAATGGGA AGGAGACTGG ATAGTTGATC CTGAAAGAAG CTTGCTGACC
    GAGGCGGATG CGGGTCACAC AGAGTTCACA GATGAAGTGT ACCAGAACGA GAGTCGCTAC
    CCCGGGGGCG AGTGGAAGCC GGCGGAGGAC ACCTACACCG ATGCGAACGG CGATAAAGCA
    GCATCACCAA GCGAGCTCAC TTGCCCTCCA GGCTGGGAAT GGGAGGATGA TGCATGGGTC
    TATGACATTA ATCGTGCAGT GGATGAGAGA GGCTGGGAAT ATGGAATTAC CATTCCTCCT
    GATAATAAGC CCAAATCCTG GGTTGCAGCA GAAAAAATGT ATCATACTCA TAGACGCCGA
    AGGCTGGTCC GAAAACGCAA GAAAGATTTA ACACAATCCA CTTCGAGTAC CGCAAGGGCC
    ATGGCAGAAT TTGAAGACCG GGAGGGCTGG GAATACGCTT CTCTAATTGG CTGGAAATTT
    CACTGGAAAC AGCGTAGTTC AGATACGTTT CGCCGCAGAC GCTGGAGGAG AAAAATGGCT
    CCTTCAGAAA CACATGGTGC AGCTGCCATC TTTAAACTTG AAGGGGCCCT TGGCGCAGAC
    ACTACGGACG ATGGAGAGGA AAAGAGCGCA GAAAAGCAGA AGCACAGCGC CACCACTGTA
    TTTGGAGCAA ACACTCCCAT TGTTTCCTGT AATTTTGACA GAGTCTACAT TTACCACCTG
    CGCTGCTACA TCTATCAAGC CAGGAACCTC ATGGCTCTAG ACAAGGATAG CTTTTCAGAT
    CCATATGCTC ATGTCTCCTT CCTTCATCGA AGTAAAACCA CCGAGATCAT CCACTCAACT
    CTGAATCCGA CATGGGACCA AACGATTATA TTCGATGAAA TTGAAATTTA TGGGGAACCC
    CAAACCATCC TACAGAACCC ACCCAAGGTT ATCATCGAAC TTTTTGACAA TGACCAAGTG
    GGCAAAGATG AATTTTTAGG ACGAAGCATG TGCTCTCCTC TGGTGAAACT AGACTCAGAA
    ATGGACATCA CTCCCAAACT TCTCTGGTAC CCAGTGATGA ATGGAGACAA AACCTGTGGG
    GATGTCCTTG TAACTGCAGA GTTGATTCTG AGGCACAAGG ATGGCTCCAA CCTTCCCATT
    CTTCCCTCGC AAAGGGCACC AAATCTGTAC ATGGTCCCCC AGGGCATCAG GCCTGTGGTT
    CAGCTCACTG CTATTGAGAT TCTAGCTTGG GGCTTAAGAA ATATGAAGAA TTACCAGATG
    GCTTCTGTCA CCTCCCCCAG TCTCATTGTG GAGTGTGGAG GGGAAAGGGT CGAGTCAGTG
    GTGATCAAAA ACCTGAAGAA GACACCCAAC TTCCCAAGTT CTGTTCTCTT CATGAGAGTG
    TTCTTGCCCA AGGAGGAACT GTACATGCCT CCACTGGTGA TCAAGGTCAT CGACCACCGG
    CAGTTCGGGC GCAAGCCCGT TGTGGGCCAG TGCACCATTG ATCGCCTGGA CCGCTTTCGC
    TGTGACCCTT ATGCAGGAAA GGAGGACATC GTGCCACAGC TAAAAGCCTC CTTTTTGTCT
    ACCCCACCCC GCCGGGACAT TGTTATTGAA ATAGAGGACA CCAAGCCATT ACTAGCTTCA
    AAGCTAACAG AAAAGGAGGA AGAAATTGTT GACTGGTGGA GTAAATTTTA TGCTTCCACA
    GGGGAACATG AAAAATGTGG ACAATATATT CAGAAAGGTT ATTCCAAACT CAAGATATAT
    GATTGTGAAC TAGAGGATGT AGAGGAATTC GAGGGCCTGA CGGACTTCTC AGATACTTTC
    AAGCTGTTTC GAGGCAAATC AGATGAGAAT GAAGACCCTT CTGTGGTAGG GGAGTTTAAG
    GGCTCCTTCC GAATCTACCC ACTGCCGGAT GACCCCAGCG TGCCGGCCCC TCCCCGGCAG
    TTTCGGGAGT TACCTGACAG CGTCCCGCAG GAATGCACGG TTAGGATTTA CATTGTTCGA
    GGCTTAGAGC TCCAGCCCCA GGACAACAAT GGCCTGTGTG ACCCTTACAT AAAAATAACA
    CTGGGCAAAA AAGTAATTGA AGACCGAGAC AACTACATCC CCAACACTCT CAACCCGGTG
    TTCGGCAGAA TGTATGAACT GAGCTGCTAC TTACCTCAAG AAAAGGACCT GAAAATTTCT
    GTCTACGATT ATGACACCTT TACCCGTGAT GAAAAAGTAG GAGAAACGAT CATTGACCTA
    GAAAACCGAT TCCTTTCCCG GTTTGGGTCC CACTGTGGCA TTCCTGAGCA GTACTGCGTT
    TCTGGAGTAA ACACCTGGCG AGATCAATTG AAACCAACAC AGTTGCTTCA AAATGTGGCC
    AGATTCAAAG GCTTCCCACC GCCCGTTCTT TCTGAAGATG GAAGTAGCAT CAGATATGGA
    GGAAAAGACT ACGGCCTAGA TGAATTTGAA GCAAACAAAA TCCTGCACCA GCACCTTGGG
    CCCCCTGAGG AGCGCCTTGC CCTCTACATC CTCAGGACTC AGGGGCTGGT TCCTGAACAC
    GTGGAAACGA GGACTTTGCA CAGCAGCTTC CAGCCTAACA TCTCCCAGGG AAGACTTCAG
    ATGTGGGTGG ATGTTTTCCC CAAGAATTTG GGGCCACCAG GCCCTCCTTT CAACATCACA
    CCCCGGAAAG CCAAGAAATA CTACCTGCGT GTGATTATCT GGAACACCAA GGATGTTGTC
    CTGGACGAGA AAAGCATCAC AGGAGAGGAA ATGAGCGACA TCTATGTGAA AGGCTGGATT
    CCTGGCAATG AAGAAAACAA ACAGAAAACA GATGTCCATT ACAGGTCCTT GGATGGCGTA
    GGGAACTTTA ACTGGAGATT CGTCTTCCCT TTTGACTACC TCCCAGCTGA ACAGCTCTGT
    CTCGTTGCTA AAAAAGAGCA TTTCTGGAGT ATTGACCAAA CTGAATTTCG GGTCCCACCC
    AGACTGGTCA TTCAGATATG GGACAATGAC AAGTTTTCTC TGGATGACTA CTTGGGTTTC
    CTGGAACTTG ATTTGCATCA TACCATCATT CCAGCAAAGT CACCAGAGAA ATGCAGTTTG
    AACATGATTC CAGACCTCAA AGCCATGAAC CCCCTGAAAG TGAAAACTGC CTCTCTCTTT
    GAGCAGAAGT CCATGAAAGG ATGGTGGCCT TGCTACACAG AGAAAGATGG CTCCCGTGTG
    ATGGCTGGGA AAGTGGAGAT GACGCTGGAA GTCCTCAATG AGAAGGAGGC CGACGAGAGG
    CCAGCTGGGA AGGGACGGGA TGAACCCAAC ATGAACCCCA AGCTGGACCC ACCAAACCGA
    CCAGAAACCT CCTTCCTCTG GTTCACCAAC CCGTGCAAGA CCATGAAGTT CATCGTGTGG
    CGCCGATTTA AGTGGGTCAT CATTGCCCTG CTGATCCTGC TGATCCTGCT GTTCTTTGTG
    GCTGTGTTTC TGTATTCCTT ACCGAACTAT TTGTCAATGA AGATTGTGAA ACCAAATGCA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48676
    Clone ID Related Accession (Same CDS sequence) XM_010819885.2
    Accession Version XM_010819885.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6144bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product myoferlin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104582.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819885.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG
    AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT
    TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG
    ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT
    TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC
    GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG
    GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT
    GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC
    CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA
    GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA
    GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC
    ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA
    CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT
    GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA AGCAAACAAA
    ATCCTGCACC AGCACCTTGG GCCCCCTGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT
    CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC
    ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA
    GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATTATC
    TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC
    ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT
    TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC
    CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA
    ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT
    CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG
    TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA
    GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA
    GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT
    GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC
    AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG
    ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG
    CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG
    AAGATTGTGA AACCAAATGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010818187.1
    CDS240..6383
    Translation

    Target ORF information:

    RefSeq Version XM_010819885.2
    Organism Bos taurus(cattle)
    Definition Bos taurus myoferlin (MYOF), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819885.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGCTTCGAG TGATTGTGGA ATCTGCCAGC AATATCCCTA AGACGAAATT TGGGAAACCG 
    GATCCTATTG TTTCTGTTAT TTTTAAGGAT GAGAAAAAGA AAACAAAGAA AGTCGATAAT
    GAACTCAACC CTGTCTGGAA TGAGATTTTG GAGTTTGACT TGCGGGGTAT ACCGCTGGAT
    TTTTCATCAT CCCTTGAGAT TGTTGTGAAA GATTTTGAGA CAATTGGACA AAATAAGTTA
    ATTGGCACGG CCACCATAGC CCTGAAGGAC CTGATGGGTG ACCAGAGCAG ATCACTGCCC
    TACAAACTGG TCTCCCTGCT AAACGAAAGG GGACAAGAGA CTGGGGCCAC AATTGACCTG
    GTGATTGGCT ATCATCCACC TTCAGCTCCA CATCCAAATG ACCCCGGTGG GACCAGCGTG
    CCAGGCACAG GAGAAGAAGA GGAAGATGGA GGTGATGAGG ACATGTTGGA CAGTACGGTC
    AGGGGCCCAG GGCCCAGGGG GCCAGGAGGG ACCGTGCCGG AAGCTCACCT TGCCCGGAGG
    CTCACGAAAG CGAAGAGCAG CCGCCGCATG CTGTCTAACA AGCCACAGGA CTTCCAGATC
    CGGGTCCGAG TGATCGAGGG CCGGCAGTTG AGTGGCAACA ACATCAAGCC TGTGGTCAAA
    GTCCACATCT GTGGCCAGAC GCATCGAACA AGAATCAAGC GAGGGAACAA TCCATTTTTT
    GATGAGTTAT TTTTCTACAA TGTCCACCTG ACTCCTTCTG AACTGATGGA TGAGATCATC
    AGCATTCGGG TTTACAATTC CCATTCTCTG CGGGCAGATT CTCTGATGGG GGAGTTTAAG
    ATCGATGTTG GATTTGTTTA TGATGAACCT GGTCATGCTG TCATGAGGAA ATGGCTTCTC
    CTCAATGACC CTGAAGATAC CAGCTCGGGT GCTAAAGGCT ACCTGAAAGT CAGCATGTTC
    GTCCTGGGAA CTGGAGACGA GCCTCCTCCT GAGAAACAAG ATCGTGATAA TGACAGTGAT
    GACGTGGAAA GTAACTTGTT ACTGCCTGCT GGCATTGCCC TGCGGTGGGT GACCTTCCTG
    CTGAAGATCT ACAGAGCGGA GGACATCCCC CAGATGGATG ATGCCTTCTC ACAGACAGTG
    AAGGAAATAT TTGGAGGCGT CGCTGATAAG AAGAACCTAG TGGACCCTTT TGTAGAAGTT
    TCCTTTGCCG GAAAAAAGGT TTGCACGAAC ATAATTGAGA AAAATGCAAA CCCTGAGTGG
    AATCAAGTCG TTAATCTTCA GATCAAGTTT CCTTCAGTGT GTGAAAAAAT AAAACTGACA
    ATATATGACT GGGACCGTCT TACCAAAAAT GATGTTGTTG GAACAACGTA TCTGCACCTC
    TCTAAAATTG CTGCCTCTGG TGGGGAAGTG GAAGCGAATA CAGGAGAAAC AGAGGTGGGC
    TTTGTTCCAA CTTTTGGACC TTGTTATCTG AACCTTTATG GAAGCCCCCG AGAGTACACG
    GGATTCCCAG ATCCCTATGA TGAGCTAAAT TCTGGAAAGG GGGAAGGAGT TGCCTACAGA
    GGCAGAATCT TGGTTGAATT AAGCACTTTA CTTGAAAAGA CACCACCAGA TAAAAAGCTT
    GAGTCCATTT CGCATGATGA CCTGCTGGTT GTTGAGAAAT ATCAGCGAAG GCGGAAGTAC
    AGCCTGTCTG CTGTGTTCCA TTCAGCCACC ATGTTGCAAG ACATTGGCGA GGCCATTCAG
    TTTGAAGTCA GCATCGGGAA CTACGGCAAC AAGTTTGACA CGACTTGTAA GCCTCTGGCA
    TCAACCACTC AGTATAGCCG TGCTGTATTT GATGGAAACT ACTATTACTA CTTGCCTTGG
    GCCCACACGA AGCCAGTTGT TACACTGACT TCCTACTGGG AGGATATTAG TCATCGCCTG
    GATGCTGTGA ACACGCTCCT AGTCATGGCA GAAAGGCTGC AATCAAACAT AGAGGCTCTA
    AAAGCAGGGA TACAAGGTAA AATTCCTTCA AACGAACTGG CAAAAATGTG GCATAAGCTC
    ATGGATGAAG TTATCGAAGA CACAAGATAC ACGTTACCTC TCACAGAAGG AAAAGCTAAT
    GTCACGGTTC TCGATACTCA GATCCGAAAG TTGCGATCCA GAGCTCTCTC TCAGATACAT
    GAGGCAGCTG TGAGGATGCG GTCTGAGGTC CCAGACGTCA AGTCCACATT GGTGGAAATT
    GAGGACTGGC TCGATAAATT AATGCAGCTG ACTGAGGAGC CTCAGAACAG CATGCCTGAC
    ATCATCATCT GGATGATCCG GGGAGAGAAG AGATTGGCCT ACGCACGCAT TCCCGCGCAT
    CAGGTTTTAT ACTCCACCAG CGGCGGGGAT GCGTCTGGGA AGTACTGTGG GAAAACCCAG
    ACCATCTTTC TGAAGTATCC ACAAGAAAAA AACAACGGAC CAAAGGTGCC CGTGGAGTTG
    CGTGTGAACA TCTGGCTGGG CTTAAGTGCT GTTGAGAAGA AGTTCAATAG CTTCGCAGAA
    GGAACATTCA CCGTCTTTGC TGAAATGTAT GAAAATCAAG CTCTCATGTT TGGAAAATGG
    GGCACTTCCG GATTAGTGGG TCGTCATAAA TTTTCCGATG TCACAGGAAA AATAAAACTC
    AAGAGGGAAT TCTTTTTGCC TCCAAAAGGC TGGGAATGGG AAGGAGACTG GATAGTTGAT
    CCTGAAAGAA GCTTGCTGAC CGAGGCGGAT GCGGGTCACA CAGAGTTCAC AGATGAAGTG
    TACCAGAACG AGAGTCGCTA CCCCGGGGGC GAGTGGAAGC CGGCGGAGGA CACCTACACC
    GATGCGAACG GCGATAAAGC AGCATCACCA AGCGAGCTCA CTTGCCCTCC AGGCTGGGAA
    TGGGAGGATG ATGCATGGGT CTATGACATT AATCGTGCAG TGGATGAGAG AGGCTGGGAA
    TATGGAATTA CCATTCCTCC TGATAATAAG CCCAAATCCT GGGTTGCAGC AGAAAAAATG
    TATCATACTC ATAGACGCCG AAGGCTGGTC CGAAAACGCA AGAAAGATTT AACACAATCC
    ACTTCGAGTA CCGCAAGGGC CATGGCAGAA TTTGAAGACC GGGAGGGCTG GGAATACGCT
    TCTCTAATTG GCTGGAAATT TCACTGGAAA CAGCGTAGTT CAGATACGTT TCGCCGCAGA
    CGCTGGAGGA GAAAAATGGC TCCTTCAGAA ACACATGGTG CAGCTGCCAT CTTTAAACTT
    GAAGGGGCCC TTGGCGCAGA CACTACGGAC GATGGAGAGG AAAAGAGCGC AGAAAAGCAG
    AAGCACAGCG CCACCACTGT ATTTGGAGCA AACACTCCCA TTGTTTCCTG TAATTTTGAC
    AGAGTCTACA TTTACCACCT GCGCTGCTAC ATCTATCAAG CCAGGAACCT CATGGCTCTA
    GACAAGGATA GCTTTTCAGA TCCATATGCT CATGTCTCCT TCCTTCATCG AAGTAAAACC
    ACCGAGATCA TCCACTCAAC TCTGAATCCG ACATGGGACC AAACGATTAT ATTCGATGAA
    ATTGAAATTT ATGGGGAACC CCAAACCATC CTACAGAACC CACCCAAGGT TATCATCGAA
    CTTTTTGACA ATGACCAAGT GGGCAAAGAT GAATTTTTAG GACGAAGCAT GTGCTCTCCT
    CTGGTGAAAC TAGACTCAGA AATGGACATC ACTCCCAAAC TTCTCTGGTA CCCAGTGATG
    AATGGAGACA AAACCTGTGG GGATGTCCTT GTAACTGCAG AGTTGATTCT GAGGCACAAG
    GATGGCTCCA ACCTTCCCAT TCTTCCCTCG CAAAGGGCAC CAAATCTGTA CATGGTCCCC
    CAGGGCATCA GGCCTGTGGT TCAGCTCACT GCTATTGAGA TTCTAGCTTG GGGCTTAAGA
    AATATGAAGA ATTACCAGAT GGCTTCTGTC ACCTCCCCCA GTCTCATTGT GGAGTGTGGA
    GGGGAAAGGG TCGAGTCAGT GGTGATCAAA AACCTGAAGA AGACACCCAA CTTCCCAAGT
    TCTGTTCTCT TCATGAGAGT GTTCTTGCCC AAGGAGGAAC TGTACATGCC TCCACTGGTG
    ATCAAGGTCA TCGACCACCG GCAGTTCGGG CGCAAGCCCG TTGTGGGCCA GTGCACCATT
    GATCGCCTGG ACCGCTTTCG CTGTGACCCT TATGCAGGAA AGGAGGACAT CGTGCCACAG
    CTAAAAGCCT CCTTTTTGTC TACCCCACCC CGCCGGGACA TTGTTATTGA AATAGAGGAC
    ACCAAGCCAT TACTAGCTTC AAAGCTAACA GAAAAGGAGG AAGAAATTGT TGACTGGTGG
    AGTAAATTTT ATGCTTCCAC AGGGGAACAT GAAAAATGTG GACAATATAT TCAGAAAGGT
    TATTCCAAAC TCAAGATATA TGATTGTGAA CTAGAGGATG TAGAGGAATT CGAGGGCCTG
    ACGGACTTCT CAGATACTTT CAAGCTGTTT CGAGGCAAAT CAGATGAGAA TGAAGACCCT
    TCTGTGGTAG GGGAGTTTAA GGGCTCCTTC CGAATCTACC CACTGCCGGA TGACCCCAGC
    GTGCCGGCCC CTCCCCGGCA GTTTCGGGAG TTACCTGACA GCGTCCCGCA GGAATGCACG
    GTTAGGATTT ACATTGTTCG AGGCTTAGAG CTCCAGCCCC AGGACAACAA TGGCCTGTGT
    GACCCTTACA TAAAAATAAC ACTGGGCAAA AAAGTAATTG AAGACCGAGA CAACTACATC
    CCCAACACTC TCAACCCGGT GTTCGGCAGA ATGTATGAAC TGAGCTGCTA CTTACCTCAA
    GAAAAGGACC TGAAAATTTC TGTCTACGAT TATGACACCT TTACCCGTGA TGAAAAAGTA
    GGAGAAACGA TCATTGACCT AGAAAACCGA TTCCTTTCCC GGTTTGGGTC CCACTGTGGC
    ATTCCTGAGC AGTACTGCGT TTCTGGAGTA AACACCTGGC GAGATCAATT GAAACCAACA
    CAGTTGCTTC AAAATGTGGC CAGATTCAAA GGCTTCCCAC CGCCCGTTCT TTCTGAAGAT
    GGAAGTAGCA TCAGATATGG AGGAAAAGAC TACGGCCTAG ATGAATTTGA AGCAAACAAA
    ATCCTGCACC AGCACCTTGG GCCCCCTGAG GAGCGCCTTG CCCTCTACAT CCTCAGGACT
    CAGGGGCTGG TTCCTGAACA CGTGGAAACG AGGACTTTGC ACAGCAGCTT CCAGCCTAAC
    ATCTCCCAGG GAAGACTTCA GATGTGGGTG GATGTTTTCC CCAAGAATTT GGGGCCACCA
    GGCCCTCCTT TCAACATCAC ACCCCGGAAA GCCAAGAAAT ACTACCTGCG TGTGATTATC
    TGGAACACCA AGGATGTTGT CCTGGACGAG AAAAGCATCA CAGGAGAGGA AATGAGCGAC
    ATCTATGTGA AAGGCTGGAT TCCTGGCAAT GAAGAAAACA AACAGAAAAC AGATGTCCAT
    TACAGGTCCT TGGATGGCGT AGGGAACTTT AACTGGAGAT TCGTCTTCCC TTTTGACTAC
    CTCCCAGCTG AACAGCTCTG TCTCGTTGCT AAAAAAGAGC ATTTCTGGAG TATTGACCAA
    ACTGAATTTC GGGTCCCACC CAGACTGGTC ATTCAGATAT GGGACAATGA CAAGTTTTCT
    CTGGATGACT ACTTGGGTTT CCTGGAACTT GATTTGCATC ATACCATCAT TCCAGCAAAG
    TCACCAGAGA AATGCAGTTT GAACATGATT CCAGACCTCA AAGCCATGAA CCCCCTGAAA
    GTGAAAACTG CCTCTCTCTT TGAGCAGAAG TCCATGAAAG GATGGTGGCC TTGCTACACA
    GAGAAAGATG GCTCCCGTGT GATGGCTGGG AAAGTGGAGA TGACGCTGGA AGTCCTCAAT
    GAGAAGGAGG CCGACGAGAG GCCAGCTGGG AAGGGACGGG ATGAACCCAA CATGAACCCC
    AAGCTGGACC CACCAAACCG ACCAGAAACC TCCTTCCTCT GGTTCACCAA CCCGTGCAAG
    ACCATGAAGT TCATCGTGTG GCGCCGATTT AAGTGGGTCA TCATTGCCCT GCTGATCCTG
    CTGATCCTGC TGTTCTTTGT GGCTGTGTTT CTGTATTCCT TACCGAACTA TTTGTCAATG
    AAGATTGTGA AACCAAATGC ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.