RNF20 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	RNF20
Entrez Gene ID	513326
Full Name	ring finger protein 20
General protein information	Preferred Names ring finger protein 20 Names E3 ubiquitin-protein ligase BRE1A BRE1-A RING-type E3 ubiquitin transferase BRE1A ring finger protein 20, E3 ubiquitin protein ligase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010808208.2	XP_010806510.1	E3 ubiquitin-protein ligase BRE1A isoform X1
XM_010808206.2	XP_010806508.1	E3 ubiquitin-protein ligase BRE1A isoform X1
XM_024995676.1	XP_024851444.1	E3 ubiquitin-protein ligase BRE1A isoform X2
NM_001081587.1	NP_001075056.1	E3 ubiquitin-protein ligase BRE1A
XM_024995675.1	XP_024851443.1	E3 ubiquitin-protein ligase BRE1A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	RNF20	XP_005626937.1
Rattus norvegicus (Norway rat)	Rnf20	NP_001101399.2
Xenopus tropicalis (tropical clawed frog)	rnf20	XP_002941911.1
Mus musculus (house mouse)	Rnf20	NP_001156735.1
Gallus gallus (chicken)	RNF20	NP_001026605.1
Pan troglodytes (chimpanzee)	RNF20	XP_520159.2
	RNF20	XP_001110807.1
Bos taurus (cattle)	RNF20	NP_001075056.1
Danio rerio (zebrafish)	rnf20	NP_001243104.1
Homo sapiens (human)	RNF20	NP_062538.5

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following RNF20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RNF20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa237049	XM_024995676.1 Latest version!	Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa43024	NM_001081587.1 Latest version!	Bos taurus ring finger protein 20 (RNF20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa22327	XM_010808206.2 Latest version!	Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa22327	XM_010808208.2 Latest version!	Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa22327	XM_024995675.1 Latest version!	Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RNF20 ( XM_024995676.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851444.1 Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237049
Clone ID Related Accession (Same CDS sequence)	XM_024995676.1
Accession Version	XM_024995676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase BRE1A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851444.1
CDS	267..3014
Translation	MLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQEKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDSEDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_024995676.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995676.1

ORF Insert Sequence:

ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( NM_001081587.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075056.1 Bos taurus ring finger protein 20 (RNF20), mRNA.

Price & Availability↑

CloneID	OBa43024
Clone ID Related Accession (Same CDS sequence)	NM_001081587.1
Accession Version	NM_001081587.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase BRE1A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133334.1. On Feb 20, 2007 this sequence version replaced XM_590991.3. ##Evidence-Data-START## Transcript exon combination :: BC133334.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END##

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ATGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075056.1
CDS	86..3013
Translation	MSGIGSKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQEKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDSEDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	NM_001081587.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 20 (RNF20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081587.1

ORF Insert Sequence:

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGATACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ATGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_010808208.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806510.1 Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa22327
Clone ID Related Accession (Same CDS sequence)	XM_010808208.2 , XM_010808206.2 , XM_024995675.1
Accession Version	XM_010808208.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104141.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808208.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806510.1
CDS	294..3221
Translation	MSGIGSKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQEKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDSEDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_010808208.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 20 (RNF20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808208.2

ORF Insert Sequence:

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_010808206.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806508.1 Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa22327
Clone ID Related Accession (Same CDS sequence)	XM_010808208.2 , XM_010808206.2 , XM_024995675.1
Accession Version	XM_010808206.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104141.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808206.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806508.1
CDS	312..3239
Translation	MSGIGSKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQEKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDSEDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_010808206.2
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808206.2

ORF Insert Sequence:

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RNF20 ( XM_024995675.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024851443.1 Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.

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CloneID	OBa22327
Clone ID Related Accession (Same CDS sequence)	XM_010808208.2 , XM_010808206.2 , XM_024995675.1
Accession Version	XM_024995675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2928bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	E3 ubiquitin-protein ligase BRE1A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024851443.1
CDS	252..3179
Translation	MSGIGSKRAAGEPGTSVPPEKKTAVEDSGTTVETIKLGGVSSTEELDIRTLQTKNRKLAEMLDQRQAIEDELREHIEKLERRQATDDASLLIVNRYWSQFDENIRIILKRYDLEQGLGDLLTERKALVVPEPEPDSDSNQERKDDRERGEGQEPAFSFLATLASSSSEEMESQLQERVESSRRAVSQIVTVYDKLQEKVELLSRKLNSGDSLMVEEAVQELNSFLAQENTRLQELTDLLQEKHCTMSQEFSKLQSKVETAESRVSVLESMIDDLQWDIDKIRKREQRLNRHLAEVLERVNSKGYKVYGAGSSLYGGTITINARKFEEMNAELEENKELAQNRHCELEKLRQDFEEVTSQNEKLKVELRSAVEEVVKETPEYRCMQSQFSVLYNESLQLKAHLDEARTLLHGTRGTHQRQVELIERDEVSLHKKLRTEVIQLEDTLAQVRKEYEMLRIEFEQTLAANEQAGPINREMRHLISSLQNHNHQLKGEVLRYKRKLREAQSDLNKTRLRSGSALLQSQSSTEDPKDEPAELKQDSEDLATQSAASKASQEEVNEIKSKRDEEERERERREKEREREREREKEKEREREKQKLKESEKERESAKDKEKGKHDDGRKKEAEIIKQLKIELKKAQESQKEMKLLLDMYRSAPKEQRDKVQLMAAEKKSKAELEDLRQRLKDLEDKEKKENKKMADEDALRKIRAVEEQIEYLQKKLAMAKQEEEALLSEMDVTGQAFEDMQEQNIRLMQQLREKDDANFKLMSERIKSNQIHKLLKEEKEELADQVLTLKTQVDAQLQVVRKLEEKEHLLQSNIGTGEKELGLRTQALEMNKRKAMEAAQLADDLKAQLEMAQKKLHDFQDEIVENSVTKEKDMFNFKRAQEDISRLRRKLETTKKPDNVPKCDEILMEEIKDYKARLTCPCCNMRKKDAVLTKCFHVFCFECVKTRYDTRQRKCPKCNAAFGANDFHRIYIG

Target ORF information:

RefSeq Version	XM_024995675.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ring finger protein 20 (RNF20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024995675.1

ORF Insert Sequence:

ATGTCAGGAA TTGGAAGTAA AAGAGCAGCT GGAGAGCCTG GCACATCTGT GCCTCCAGAG 
AAGAAGACAG CTGTTGAAGA TTCAGGGACC ACAGTGGAAA CAATTAAACT TGGGGGTGTC 
TCTTCAACGG AGGAGTTAGA CATTCGAACA CTGCAAACCA AAAATCGCAA ACTGGCAGAA 
ATGCTGGATC AACGGCAAGC CATCGAAGAT GAACTCCGTG AACACATTGA AAAACTGGAA 
CGAAGACAGG CCACTGATGA TGCCTCACTG TTGATTGTCA ACCGCTACTG GAGTCAGTTT 
GATGAAAACA TCCGTATCAT CCTTAAGCGT TATGATCTGG AGCAAGGTTT GGGAGACCTA 
CTCACAGAGA GAAAAGCCCT TGTTGTGCCC GAGCCAGAAC CAGACTCTGA TAGCAATCAA 
GAACGGAAAG ATGACCGAGA GAGAGGGGAA GGGCAAGAGC CAGCTTTCTC TTTCCTTGCT 
ACTTTGGCCA GCAGTTCCAG TGAAGAGATG GAATCTCAGC TGCAGGAGCG CGTGGAGTCC 
TCCCGCCGAG CGGTGTCCCA GATTGTGACC GTCTATGATA AACTGCAAGA AAAGGTGGAG 
CTCTTATCAC GGAAGCTGAA CAGTGGAGAC AGTCTGATGG TAGAGGAAGC AGTGCAGGAG 
CTGAATTCCT TCCTTGCACA AGAGAACACA AGGCTGCAAG AACTGACAGA CCTCCTTCAG 
GAGAAGCATT GCACCATGTC TCAGGAGTTT TCCAAGTTGC AGAGTAAAGT GGAGACAGCT 
GAGTCACGAG TGTCTGTCCT GGAGTCCATG ATCGATGACC TGCAGTGGGA TATTGACAAA 
ATTCGAAAGC GGGAACAGCG ACTTAACCGA CACTTAGCAG AAGTCTTAGA ACGGGTAAAT 
TCAAAAGGTT ATAAAGTGTA CGGTGCAGGG AGCAGCCTCT ACGGTGGTAC AATCACCATC 
AACGCCCGGA AGTTTGAGGA AATGAATGCG GAGCTTGAAG AGAACAAAGA GTTGGCTCAG 
AACCGTCACT GTGAGCTGGA GAAACTTCGG CAAGACTTCG AGGAGGTCAC TTCACAAAAT 
GAAAAACTGA AGGTGGAATT ACGGAGCGCA GTGGAGGAAG TGGTCAAGGA GACTCCAGAA 
TATCGCTGCA TGCAGTCACA GTTCTCCGTG CTGTACAACG AGAGTCTGCA GTTGAAAGCA 
CACTTGGATG AGGCTCGGAC CCTGCTTCAT GGCACTAGGG GAACCCACCA ACGTCAGGTG 
GAGCTCATTG AGCGAGATGA GGTTAGTCTT CATAAGAAGC TGAGGACGGA AGTAATCCAG 
CTGGAAGATA CACTGGCCCA GGTCCGCAAG GAGTATGAGA TGCTGAGGAT AGAATTCGAG 
CAGACCCTTG CTGCCAATGA ACAAGCAGGC CCCATAAACC GGGAGATGCG CCACCTCATC 
AGTAGCCTCC AGAATCACAA CCACCAGCTG AAAGGGGAGG TCCTAAGGTA TAAGCGGAAA 
CTGAGAGAAG CCCAGTCTGA CCTGAACAAG ACTCGGCTGC GCAGTGGCAG TGCCCTCCTG 
CAGTCTCAGT CCAGTACTGA GGACCCTAAG GACGAGCCTG CAGAGCTAAA ACAGGATTCT 
GAGGACTTAG CCACCCAGTC TGCTGCATCG AAGGCATCTC AGGAGGAGGT CAACGAAATT 
AAGTCCAAAC GCGATGAGGA AGAGCGAGAA CGAGAAAGGA GGGAGAAAGA GAGGGAGCGA 
GAAAGAGAGC GGGAGAAGGA GAAGGAGCGA GAACGAGAGA AGCAGAAACT AAAAGAGTCA 
GAAAAAGAGA GAGAGTCTGC TAAGGATAAA GAGAAAGGGA AACATGATGA TGGAAGGAAA 
AAGGAAGCGG AAATTATCAA ACAATTGAAG ATTGAACTCA AGAAAGCACA GGAGAGCCAA 
AAGGAGATGA AACTGTTGCT GGATATGTAC CGCTCTGCCC CAAAGGAGCA GAGAGACAAG 
GTTCAGCTGA TGGCAGCAGA GAAGAAGTCT AAGGCAGAGT TGGAAGATTT AAGACAAAGA 
CTCAAGGATC TGGAGGATAA GGAGAAAAAA GAGAATAAGA AAATGGCTGA TGAAGATGCC 
TTAAGAAAGA TCCGGGCAGT AGAGGAGCAG ATAGAATACC TCCAGAAGAA GCTGGCCATG 
GCCAAGCAGG AAGAAGAAGC TCTCCTCTCT GAGATGGACG TCACAGGCCA GGCCTTTGAA 
GATATGCAGG AGCAAAATAT CCGTTTGATG CAGCAGCTGA GGGAGAAGGA TGATGCAAAC 
TTCAAGCTCA TGTCAGAGCG TATCAAATCC AATCAGATCC ATAAGCTGCT GAAAGAAGAG 
AAGGAGGAGC TGGCAGACCA AGTTTTGACT CTGAAGACTC AGGTTGATGC CCAGTTACAG 
GTAGTGAGGA AACTGGAAGA GAAGGAGCAC CTGTTGCAGA GCAACATCGG CACAGGGGAG 
AAGGAGCTGG GTCTTAGGAC CCAGGCCTTG GAGATGAATA AACGCAAGGC AATGGAGGCA 
GCCCAGCTTG CAGATGACCT CAAAGCACAG TTGGAGATGG CTCAGAAGAA GCTACATGAT 
TTTCAGGATG AGATTGTAGA GAACAGTGTC ACCAAAGAAA AAGATATGTT CAATTTCAAA 
CGGGCCCAGG AGGACATCTC TAGACTTCGA AGGAAGCTGG AGACCACGAA GAAACCAGAC 
AATGTACCCA AATGTGATGA GATTCTGATG GAGGAGATTA AGGACTACAA GGCACGCCTG 
ACCTGTCCGT GTTGCAACAT GCGTAAAAAG GATGCTGTGC TTACCAAGTG TTTTCATGTT 
TTCTGCTTTG AGTGTGTTAA GACCCGCTAT GATACCCGCC AGCGCAAATG TCCCAAGTGT 
AATGCTGCCT TTGGTGCCAA CGATTTCCAT CGCATCTACA TAGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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RNF20 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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