SSH2 cDNA ORF clone, Bos taurus(cattle)

The following SSH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SSH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa10027 XM_005220145.4
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa10029 XM_005220147.4
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa10030 XM_002695677.6
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa226719 XM_015458587.2
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226720 XM_024980694.1
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa226721 XM_015458589.2
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa93790 XM_015467983.1
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93790 XM_015458587.1
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa93791 XM_015458589.1
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa93791 XM_015467984.1
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa29558 XM_005220145.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29558 XM_005195611.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29560 XM_005195613.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29560 XM_005220147.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29561 XM_002695677.5
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29561 XM_590201.8
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29562 XM_005195614.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa29562 XM_005220148.3
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa48385 XM_010824015.2
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa48385 XM_010815867.2
Latest version!
Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa10027
    Clone ID Related Accession (Same CDS sequence) XM_005220145.4
    Accession Version XM_005220145.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220145.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATTGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAACTGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220202.2
    CDS1..4290
    Translation

    Target ORF information:

    RefSeq Version XM_005220145.4
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220145.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATTGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAACTGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10029
    Clone ID Related Accession (Same CDS sequence) XM_005220147.4
    Accession Version XM_005220147.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005220147.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATTGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAACTGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220204.2
    CDS213..4442
    Translation

    Target ORF information:

    RefSeq Version XM_005220147.4
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220147.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATTGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAACTGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa10030
    Clone ID Related Accession (Same CDS sequence) XM_002695677.6
    Accession Version XM_002695677.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002695677.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATTGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAACTGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695723.3
    CDS31..4239
    Translation

    Target ORF information:

    RefSeq Version XM_002695677.6
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695677.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATTGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAACTGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226719
    Clone ID Related Accession (Same CDS sequence) XM_015458587.2
    Accession Version XM_015458587.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TTGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAACTGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314073.2
    CDS41..4327
    Translation

    Target ORF information:

    RefSeq Version XM_015458587.2
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458587.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TTGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAACTGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226720
    Clone ID Related Accession (Same CDS sequence) XM_024980694.1
    Accession Version XM_024980694.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TTGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAACTGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836462.1
    CDS184..4410
    Translation

    Target ORF information:

    RefSeq Version XM_024980694.1
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024980694.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TTGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAACTGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa226721
    Clone ID Related Accession (Same CDS sequence) XM_015458589.2
    Accession Version XM_015458589.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015458589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATTGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAC TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314075.2
    CDS174..3806
    Translation

    Target ORF information:

    RefSeq Version XM_015458589.2
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458589.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATTGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAC TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93790
    Clone ID Related Accession (Same CDS sequence) XM_015458587.1 , XM_015467983.1
    Accession Version XM_015458587.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314073.1
    CDS71..4357
    Translation

    Target ORF information:

    RefSeq Version XM_015458587.1
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458587.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93790
    Clone ID Related Accession (Same CDS sequence) XM_015458587.1 , XM_015467983.1
    Accession Version XM_015467983.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4287bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323469.1
    CDS71..4357
    Translation

    Target ORF information:

    RefSeq Version XM_015467983.1
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467983.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93791
    Clone ID Related Accession (Same CDS sequence) XM_015467984.1 , XM_015458589.1
    Accession Version XM_015467984.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATCGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAA TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015323470.1
    CDS151..3783
    Translation

    Target ORF information:

    RefSeq Version XM_015467984.1
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015467984.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATCGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAA TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa93791
    Clone ID Related Accession (Same CDS sequence) XM_015467984.1 , XM_015458589.1
    Accession Version XM_015458589.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATCGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAA TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314075.1
    CDS151..3783
    Translation

    Target ORF information:

    RefSeq Version XM_015458589.1
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458589.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCATAACT ACTACCCCGG CAGCCTGTTT CTCACCTGGG TGAGTTACTA TGAGAGCCAT 
    ATCAACTCAG ACCAGTCCTC GGTCAACGAA TGGAATGCTA TGCAGGATGT ACAGTCCCAC
    CGACCTGACT CTCCTGCTCT CTTCACAGAC ATACCAACTG AACGTGAGCG GACAGAGAGG
    CTAATTAAAA CCAGATTAAG GGAGATTATG ATGCAAAAGG ATTTGGAGAA TATCACATCG
    AAAGAGATAC GAACTGAACT AGAAATGCAG ATGGTGTGCA ACTTGCGAGA ATTCAAGGAA
    TTTATAGATA ATGAAATGAT AGTGATCCTT GGTCAGATGG ATAGCCCTAC ACAGATATTC
    GAGCACGTGT TCTTGGGCTC AGAATGGAAT GCCTCCAACT TAGAGGATTT ACAGAACCGA
    GGGGTTCGCT ATATCTTGAA TGTCACTCGA GAGATAGACA ACTTCTTCCC GGGAGTCTTT
    GAGTATCACA ACATTCGGGT GTATGATGAA GAGGCAACCG ACCTCCTGGC TTACTGGAAT
    GACACTTACA AATTCATCTC TAAAGCAAAG AAACATGGAT CTAAATGCCT CGTGCACTGC
    AAAATGGGGG TGAGTCGCTC GGCCTCCACT GTGATCGCCT ATGCGATGAA GGAGTATGGC
    TGGAACTTGG ACCGAGCCTA TGACTATGTG AAGGAAAGAC GGACAGTGAC CAAGCCCAAC
    CCCAGCTTCA TGAGACAGCT GGAAGAGTAC CAGGGGATCT TGCTGGCAAG CAAACAGCGG
    CACAACAAAC TCTGGAGATC GCATTCAGAT AGCGACCTCT CGGACCACCA CGAACCCATC
    TGCAAACCAG GACTGGAACT CAACAAAAAG GAGATTACCA CCTCAGCAGA CCAGATTGCC
    GAGGTGAAGA CCATGGAGAG CCACCCACCC ATCCCTCCCG TCTTTGTGGA ACATGTGGTC
    CCGCAGGATG AGAATCAGAA AGCCCTGTGC ACCAAAGAAA GAATGATCTG CTTGGAGTTT
    ACATCTCAGG AATTTCACGC CGGACAGATT GAGGATGAAT TAAACCTAAA TGACATCAAT
    GGATGCTCAT CAGGGTGTTG TCTCAATGAA CCGAAATTCC CTCTTGACAA CTGCCATGCA
    TCCAAAGCCT TAATCCAACC TGGAAAGGAC CCAGAAATGG CCCACAAGTT CCCAGACTTA
    ACTGTGGAAG ATCTGGAGAC GGACGCGCTG AAAGCAGACA TGAATGTCCA CCTGCTGCCC
    ATGGAAGAGC TGACATCCCG CCTGAAAGAT CTCCCCATGT CCCCGGATCC TGAGTCACCA
    AGCCCCCAAC CCAGCTGCCA GGCCACAGTC TCAGATTTCA GTGGAGATCG CATTGACTTT
    TTCAGCGCTC TGGAGAAGTT TGTAGAGCTT TCCCAAGAAA CCCGGTCCCG ATCTTTTTCT
    CATTCAAGGA TGGAGGAAGT GGGTGGAGGA AGGAATGAGA GCTGTCGACT GTCAGTGGTG
    GAAGCAGCCC CTTCTGAAGC GACCACTGAT GACCAGAGAA GCAGCTCTTT GAGTAATACT
    CCCCACGCAT CTGAGGAATC TTCAGTAGAT GAGGAGCAGT CAAAGGCAAT CTCAGAACAG
    GTCAGCCCGG ACATCTTCAT GCAGCCGCAC TCAGAAAATG CGATGTCAGT CAAAGAAATC
    GTCACTGAGA TTGAATCCAT CAGTCAAGGA GTTGGACAGA TTCAAGTGAA AGGAGACGTG
    CTGTCCAACC CATGCCACAC ACCAAAGAAG CACATCGTCC ACGAGCTGCC CCTGGAGAGG
    GCCCAGGCCG CCGAGAACAG ACCTGGAAAC CTGGAGCAGA GTGAAGGTTC CTGCCCAGCC
    CAGCCTGAAC CAGCCAAAGA CTCAGGGAAG GGGGACCCCG AGGGGTGCCC AGGGGCACAC
    TCCTCCACCG CAGAGGTAGA AGAAGAGGAA CCAGTGGAGG GGGAACAAGA GCTCTGGGGC
    CCCGGGATGC CCCCGGGTGC CAAGTGGTAC CCCGGGTCCG TGAGGCGAGC CACCTTGGAG
    TTTGAGGAGC GCCTGCGGCA AGAACAGGAG CACCATGGTG CTGCCCCTGC GGGGACCTCG
    CTGTCCACTC GCAAGAATTC CAAGAACGAT TCTTCAGTGG CAGACCTGGC AGCGAAAGGG
    AAGAGTGACG AAGCCGCCCC AGAACTGTCA TATGTCCCCA AGGAGCCAGA GACGGGCAGG
    GGCCAAGGGC GATGCGGTGG GTCTGAGTCT GGCTCTTTAC TGCGTCCTGA GCAGCTTGCC
    ATCGTCCCGG CCCCTGAGCT GCGGGAGTCT CACCCAGCGC CAGCAACTCA GGATGGCCCA
    CGGCATGAAG GTATCCTGCG GGAGCCGAGG ACTGTGGTCT CCGGCCAGGG GCCCGAGACA
    CCAGCGGCCC CTGCTGCCTT TCCCAAGAAG ATAGAAATCA TTGAATACAC CTACACAGCC
    ACATCCCCTG ATCCCGGGCC AGGGTGGGAA AGAGCCAGCA GTGAGAAGAG TGGGGCGCAG
    GGACTGAGGA CAGTGAAGGT GGAAGAGACC CTCACTGTCC TCTGTGCTCT GGATGAAAAT
    CTGAACCGGA CGCTGAGCCC CGACCAGGCT CCTCTGCACC CCAGAGTGCT ACCTCCGCCT
    CATTCTTCCT CTCCCCAGCA TGAGCACAGC AGGCCAGTCG ACCTGCCCCC CACCCTGAGC
    AGCCCAGCAG CGCCTATTGT CAGTCCCACG ACCCAGCCAT GTGGCGCCAG CCCGGATCAC
    CTGCACCCCC AGACGGTGGT TCACCTGGAA GGCTTCACAG GGCAGAGCAG CACCACAGAC
    AGCGAGCCCT CCACGGAGCA GGGCAGCTGG GAAGAAAGTC AGGAGGGCCC CCTCTCCAGG
    GCCAGTGAAG TGCCATATCA GGGCTCCCAG TTCAGTAGCG AAGACCTGCA TTTAATCAGC
    CAACTGGGTA ATAATATCGG GGAGCAACCA GAAAAACTGG ACCCAACATC TGTAGCCCAT
    CAGCTCCCAC ACAGCTCCAG TAGCGACAGT ATAGAGAGTC CCAGTCAGAG CCCCAGGGTG
    GTGAAGGGAC GCGCTAAAGA AATCGAGTCC CGAGCAACTT CCCAGGTAGG GCTCCCCAAG
    CCACCCCAAA TGAGGCGTTC AGCTTCCCTT GCCAAGTTAG GTTACTTGGA CCTCTGTAAA
    GACTGTTCAC CAGAGAGGGA GCCTGTCTCC TCTGAGTCCC CTCATCTCAA ACTGCTTCAG
    CCCTTCCTCA GAACGGACTC GGGCATGGAG GCCCAGGAGC CCCCAGAAAA CCCAGACACT
    CCCCAGAACC GAGAGCCCAC CAAGTATTTG ATAGAGCAGC TCAGAACCAC AGAGTGCATC
    GCGCAGAGCA GGCCAGTGGA GAGGCCCCTT GCACAGTATG CCAAAGAGTT CGGTTCCAGT
    CAGCAGTGTT TGCTCCCCAG GGCAGGACCT GGATTGACTA GTTCGGAAGG AGGCCTTCCT
    TCGGTACCGA CCCAGGGACT GCAGGACACC AGCCCGGCCC CGGGGCTGGC TGTGGCGCCC
    CGAGAGCAGC ACGGCAGAAC TCACCCCCTT AGGAGACTGA GAAAAGCAAA TGATAAAAAA
    CGGACAACCA ACCCCTTCTA TAATACCATG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29558
    Clone ID Related Accession (Same CDS sequence) XM_005195611.3 , XM_005220145.3
    Accession Version XM_005195611.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195611.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195668.2
    CDS70..4359
    Translation

    Target ORF information:

    RefSeq Version XM_005195611.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195611.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29558
    Clone ID Related Accession (Same CDS sequence) XM_005195611.3 , XM_005220145.3
    Accession Version XM_005220145.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220145.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220202.1
    CDS70..4359
    Translation

    Target ORF information:

    RefSeq Version XM_005220145.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220145.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGAGCTCTC ACCTGGAAGA CAGTGAATCC GCAGCATTAC TTTGCTGTGA ATGTGAAGAC
    TCAGAAATCT TTAGTGATTC CAATGAGGCA GACAGTGGGG AGGAGGAATG CCGATCACAG
    CCCAGGAGCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29560
    Clone ID Related Accession (Same CDS sequence) XM_005195613.3 , XM_005220147.3
    Accession Version XM_005195613.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195670.2
    CDS179..4408
    Translation

    Target ORF information:

    RefSeq Version XM_005195613.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195613.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29560
    Clone ID Related Accession (Same CDS sequence) XM_005195613.3 , XM_005220147.3
    Accession Version XM_005220147.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220204.1
    CDS179..4408
    Translation

    Target ORF information:

    RefSeq Version XM_005220147.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220147.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGACCCTGA GCACGTTGGC CCGCGAGAAG AAGGCGCCCC TCGCCTGCAC CTGCAGCCTC 
    GGTGGCCCCG ACATGATCCC CTACTTCTCC GCCAACGCGG TCATCTCGCA GAACGCCATC
    AACCAGCTCA TCAGCGAGAG CTTTCTAACT GTCAAAGGTG CTGCCCTTTT TCTACCCCGG
    GGAAATGGCT CATCCACACC AAGAGTCAGC CACAGACGCA ACAAGCATGC AGGTGATCTC
    CAACAGCATC TCCAAGCAAT GTTCATACTA CTTCGCCCAG AAGACAACAT CAGGCTGGCT
    GTAAGACTAG AAAGTACTTA CCAGAATCGA ACACGCTATA TGGTAGTGGT TTCAACTAAT
    GGTAGACAAG ACACTGAAGA AAGCATCGTC CTAGGAATGG ATTTCTCCTC TAATGACAGT
    AGCACTTGTA CCATGGGCTT AGTCCTGCCT CTCTGGAGTG ACACCCTAAT CCATTTGGAT
    GGTGATGGTG GGTTCAGCGT CTCGACAGAT AACAGAGTTC ACATATTCAA ACCTGTATCT
    GTGCAGGCAA TGTGGTCTGC ACTGCAGAGT TTACACAAGG CTTGTGAAGT GGCCAGAATG
    CATAACTACT ACCCCGGCAG CCTGTTTCTC ACCTGGGTGA GTTACTATGA GAGCCATATC
    AACTCAGACC AGTCCTCGGT CAACGAATGG AATGCTATGC AGGATGTACA GTCCCACCGA
    CCTGACTCTC CTGCTCTCTT CACAGACATA CCAACTGAAC GTGAGCGGAC AGAGAGGCTA
    ATTAAAACCA GATTAAGGGA GATTATGATG CAAAAGGATT TGGAGAATAT CACATCGAAA
    GAGATACGAA CTGAACTAGA AATGCAGATG GTGTGCAACT TGCGAGAATT CAAGGAATTT
    ATAGATAATG AAATGATAGT GATCCTTGGT CAGATGGATA GCCCTACACA GATATTCGAG
    CACGTGTTCT TGGGCTCAGA ATGGAATGCC TCCAACTTAG AGGATTTACA GAACCGAGGG
    GTTCGCTATA TCTTGAATGT CACTCGAGAG ATAGACAACT TCTTCCCGGG AGTCTTTGAG
    TATCACAACA TTCGGGTGTA TGATGAAGAG GCAACCGACC TCCTGGCTTA CTGGAATGAC
    ACTTACAAAT TCATCTCTAA AGCAAAGAAA CATGGATCTA AATGCCTCGT GCACTGCAAA
    ATGGGGGTGA GTCGCTCGGC CTCCACTGTG ATCGCCTATG CGATGAAGGA GTATGGCTGG
    AACTTGGACC GAGCCTATGA CTATGTGAAG GAAAGACGGA CAGTGACCAA GCCCAACCCC
    AGCTTCATGA GACAGCTGGA AGAGTACCAG GGGATCTTGC TGGCAAGCAA ACAGCGGCAC
    AACAAACTCT GGAGATCGCA TTCAGATAGC GACCTCTCGG ACCACCACGA ACCCATCTGC
    AAACCAGGAC TGGAACTCAA CAAAAAGGAG ATTACCACCT CAGCAGACCA GATTGCCGAG
    GTGAAGACCA TGGAGAGCCA CCCACCCATC CCTCCCGTCT TTGTGGAACA TGTGGTCCCG
    CAGGATGAGA ATCAGAAAGC CCTGTGCACC AAAGAAAGAA TGATCTGCTT GGAGTTTACA
    TCTCAGGAAT TTCACGCCGG ACAGATTGAG GATGAATTAA ACCTAAATGA CATCAATGGA
    TGCTCATCAG GGTGTTGTCT CAATGAACCG AAATTCCCTC TTGACAACTG CCATGCATCC
    AAAGCCTTAA TCCAACCTGG AAAGGACCCA GAAATGGCCC ACAAGTTCCC AGACTTAACT
    GTGGAAGATC TGGAGACGGA CGCGCTGAAA GCAGACATGA ATGTCCACCT GCTGCCCATG
    GAAGAGCTGA CATCCCGCCT GAAAGATCTC CCCATGTCCC CGGATCCTGA GTCACCAAGC
    CCCCAACCCA GCTGCCAGGC CACAGTCTCA GATTTCAGTG GAGATCGCAT TGACTTTTTC
    AGCGCTCTGG AGAAGTTTGT AGAGCTTTCC CAAGAAACCC GGTCCCGATC TTTTTCTCAT
    TCAAGGATGG AGGAAGTGGG TGGAGGAAGG AATGAGAGCT GTCGACTGTC AGTGGTGGAA
    GCAGCCCCTT CTGAAGCGAC CACTGATGAC CAGAGAAGCA GCTCTTTGAG TAATACTCCC
    CACGCATCTG AGGAATCTTC AGTAGATGAG GAGCAGTCAA AGGCAATCTC AGAACAGGTC
    AGCCCGGACA TCTTCATGCA GCCGCACTCA GAAAATGCGA TGTCAGTCAA AGAAATCGTC
    ACTGAGATTG AATCCATCAG TCAAGGAGTT GGACAGATTC AAGTGAAAGG AGACGTGCTG
    TCCAACCCAT GCCACACACC AAAGAAGCAC ATCGTCCACG AGCTGCCCCT GGAGAGGGCC
    CAGGCCGCCG AGAACAGACC TGGAAACCTG GAGCAGAGTG AAGGTTCCTG CCCAGCCCAG
    CCTGAACCAG CCAAAGACTC AGGGAAGGGG GACCCCGAGG GGTGCCCAGG GGCACACTCC
    TCCACCGCAG AGGTAGAAGA AGAGGAACCA GTGGAGGGGG AACAAGAGCT CTGGGGCCCC
    GGGATGCCCC CGGGTGCCAA GTGGTACCCC GGGTCCGTGA GGCGAGCCAC CTTGGAGTTT
    GAGGAGCGCC TGCGGCAAGA ACAGGAGCAC CATGGTGCTG CCCCTGCGGG GACCTCGCTG
    TCCACTCGCA AGAATTCCAA GAACGATTCT TCAGTGGCAG ACCTGGCAGC GAAAGGGAAG
    AGTGACGAAG CCGCCCCAGA ACTGTCATAT GTCCCCAAGG AGCCAGAGAC GGGCAGGGGC
    CAAGGGCGAT GCGGTGGGTC TGAGTCTGGC TCTTTACTGC GTCCTGAGCA GCTTGCCATC
    GTCCCGGCCC CTGAGCTGCG GGAGTCTCAC CCAGCGCCAG CAACTCAGGA TGGCCCACGG
    CATGAAGGTA TCCTGCGGGA GCCGAGGACT GTGGTCTCCG GCCAGGGGCC CGAGACACCA
    GCGGCCCCTG CTGCCTTTCC CAAGAAGATA GAAATCATTG AATACACCTA CACAGCCACA
    TCCCCTGATC CCGGGCCAGG GTGGGAAAGA GCCAGCAGTG AGAAGAGTGG GGCGCAGGGA
    CTGAGGACAG TGAAGGTGGA AGAGACCCTC ACTGTCCTCT GTGCTCTGGA TGAAAATCTG
    AACCGGACGC TGAGCCCCGA CCAGGCTCCT CTGCACCCCA GAGTGCTACC TCCGCCTCAT
    TCTTCCTCTC CCCAGCATGA GCACAGCAGG CCAGTCGACC TGCCCCCCAC CCTGAGCAGC
    CCAGCAGCGC CTATTGTCAG TCCCACGACC CAGCCATGTG GCGCCAGCCC GGATCACCTG
    CACCCCCAGA CGGTGGTTCA CCTGGAAGGC TTCACAGGGC AGAGCAGCAC CACAGACAGC
    GAGCCCTCCA CGGAGCAGGG CAGCTGGGAA GAAAGTCAGG AGGGCCCCCT CTCCAGGGCC
    AGTGAAGTGC CATATCAGGG CTCCCAGTTC AGTAGCGAAG ACCTGCATTT AATCAGCCAA
    CTGGGTAATA ATATCGGGGA GCAACCAGAA AAACTGGACC CAACATCTGT AGCCCATCAG
    CTCCCACACA GCTCCAGTAG CGACAGTATA GAGAGTCCCA GTCAGAGCCC CAGGGTGGTG
    AAGGGACGCG CTAAAGAAAT CGAGTCCCGA GCAACTTCCC AGGTAGGGCT CCCCAAGCCA
    CCCCAAATGA GGCGTTCAGC TTCCCTTGCC AAGTTAGGTT ACTTGGACCT CTGTAAAGAC
    TGTTCACCAG AGAGGGAGCC TGTCTCCTCT GAGTCCCCTC ATCTCAAACT GCTTCAGCCC
    TTCCTCAGAA CGGACTCGGG CATGGAGGCC CAGGAGCCCC CAGAAAACCC AGACACTCCC
    CAGAACCGAG AGCCCACCAA GTATTTGATA GAGCAGCTCA GAACCACAGA GTGCATCGCG
    CAGAGCAGGC CAGTGGAGAG GCCCCTTGCA CAGTATGCCA AAGAGTTCGG TTCCAGTCAG
    CAGTGTTTGC TCCCCAGGGC AGGACCTGGA TTGACTAGTT CGGAAGGAGG CCTTCCTTCG
    GTACCGACCC AGGGACTGCA GGACACCAGC CCGGCCCCGG GGCTGGCTGT GGCGCCCCGA
    GAGCAGCACG GCAGAACTCA CCCCCTTAGG AGACTGAGAA AAGCAAATGA TAAAAAACGG
    ACAACCAACC CCTTCTATAA TACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29561
    Clone ID Related Accession (Same CDS sequence) XM_590201.8 , XM_002695677.5
    Accession Version XM_590201.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_590201.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_590201.6
    CDS69..4277
    Translation

    Target ORF information:

    RefSeq Version XM_590201.8
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_590201.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29561
    Clone ID Related Accession (Same CDS sequence) XM_590201.8 , XM_002695677.5
    Accession Version XM_002695677.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4209bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002695677.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002695723.2
    CDS69..4277
    Translation

    Target ORF information:

    RefSeq Version XM_002695677.5
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002695677.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTTGG TCACGGTCCA GCGGTCGCCT ACCCCCAGCA CTACCTCCAG CCCCTGCGCC 
    TCGGAGGCAG ACAGTGGGGA GGAGGAATGC CGATCACAGC CCAGGAGCAT CAGCGAGAGC
    TTTCTAACTG TCAAAGGTGC TGCCCTTTTT CTACCCCGGG GAAATGGCTC ATCCACACCA
    AGAGTCAGCC ACAGACGCAA CAAGCATGCA GGTGATCTCC AACAGCATCT CCAAGCAATG
    TTCATACTAC TTCGCCCAGA AGACAACATC AGGCTGGCTG TAAGACTAGA AAGTACTTAC
    CAGAATCGAA CACGCTATAT GGTAGTGGTT TCAACTAATG GTAGACAAGA CACTGAAGAA
    AGCATCGTCC TAGGAATGGA TTTCTCCTCT AATGACAGTA GCACTTGTAC CATGGGCTTA
    GTCCTGCCTC TCTGGAGTGA CACCCTAATC CATTTGGATG GTGATGGTGG GTTCAGCGTC
    TCGACAGATA ACAGAGTTCA CATATTCAAA CCTGTATCTG TGCAGGCAAT GTGGTCTGCA
    CTGCAGAGTT TACACAAGGC TTGTGAAGTG GCCAGAATGC ATAACTACTA CCCCGGCAGC
    CTGTTTCTCA CCTGGGTGAG TTACTATGAG AGCCATATCA ACTCAGACCA GTCCTCGGTC
    AACGAATGGA ATGCTATGCA GGATGTACAG TCCCACCGAC CTGACTCTCC TGCTCTCTTC
    ACAGACATAC CAACTGAACG TGAGCGGACA GAGAGGCTAA TTAAAACCAG ATTAAGGGAG
    ATTATGATGC AAAAGGATTT GGAGAATATC ACATCGAAAG AGATACGAAC TGAACTAGAA
    ATGCAGATGG TGTGCAACTT GCGAGAATTC AAGGAATTTA TAGATAATGA AATGATAGTG
    ATCCTTGGTC AGATGGATAG CCCTACACAG ATATTCGAGC ACGTGTTCTT GGGCTCAGAA
    TGGAATGCCT CCAACTTAGA GGATTTACAG AACCGAGGGG TTCGCTATAT CTTGAATGTC
    ACTCGAGAGA TAGACAACTT CTTCCCGGGA GTCTTTGAGT ATCACAACAT TCGGGTGTAT
    GATGAAGAGG CAACCGACCT CCTGGCTTAC TGGAATGACA CTTACAAATT CATCTCTAAA
    GCAAAGAAAC ATGGATCTAA ATGCCTCGTG CACTGCAAAA TGGGGGTGAG TCGCTCGGCC
    TCCACTGTGA TCGCCTATGC GATGAAGGAG TATGGCTGGA ACTTGGACCG AGCCTATGAC
    TATGTGAAGG AAAGACGGAC AGTGACCAAG CCCAACCCCA GCTTCATGAG ACAGCTGGAA
    GAGTACCAGG GGATCTTGCT GGCAAGCAAA CAGCGGCACA ACAAACTCTG GAGATCGCAT
    TCAGATAGCG ACCTCTCGGA CCACCACGAA CCCATCTGCA AACCAGGACT GGAACTCAAC
    AAAAAGGAGA TTACCACCTC AGCAGACCAG ATTGCCGAGG TGAAGACCAT GGAGAGCCAC
    CCACCCATCC CTCCCGTCTT TGTGGAACAT GTGGTCCCGC AGGATGAGAA TCAGAAAGCC
    CTGTGCACCA AAGAAAGAAT GATCTGCTTG GAGTTTACAT CTCAGGAATT TCACGCCGGA
    CAGATTGAGG ATGAATTAAA CCTAAATGAC ATCAATGGAT GCTCATCAGG GTGTTGTCTC
    AATGAACCGA AATTCCCTCT TGACAACTGC CATGCATCCA AAGCCTTAAT CCAACCTGGA
    AAGGACCCAG AAATGGCCCA CAAGTTCCCA GACTTAACTG TGGAAGATCT GGAGACGGAC
    GCGCTGAAAG CAGACATGAA TGTCCACCTG CTGCCCATGG AAGAGCTGAC ATCCCGCCTG
    AAAGATCTCC CCATGTCCCC GGATCCTGAG TCACCAAGCC CCCAACCCAG CTGCCAGGCC
    ACAGTCTCAG ATTTCAGTGG AGATCGCATT GACTTTTTCA GCGCTCTGGA GAAGTTTGTA
    GAGCTTTCCC AAGAAACCCG GTCCCGATCT TTTTCTCATT CAAGGATGGA GGAAGTGGGT
    GGAGGAAGGA ATGAGAGCTG TCGACTGTCA GTGGTGGAAG CAGCCCCTTC TGAAGCGACC
    ACTGATGACC AGAGAAGCAG CTCTTTGAGT AATACTCCCC ACGCATCTGA GGAATCTTCA
    GTAGATGAGG AGCAGTCAAA GGCAATCTCA GAACAGGTCA GCCCGGACAT CTTCATGCAG
    CCGCACTCAG AAAATGCGAT GTCAGTCAAA GAAATCGTCA CTGAGATTGA ATCCATCAGT
    CAAGGAGTTG GACAGATTCA AGTGAAAGGA GACGTGCTGT CCAACCCATG CCACACACCA
    AAGAAGCACA TCGTCCACGA GCTGCCCCTG GAGAGGGCCC AGGCCGCCGA GAACAGACCT
    GGAAACCTGG AGCAGAGTGA AGGTTCCTGC CCAGCCCAGC CTGAACCAGC CAAAGACTCA
    GGGAAGGGGG ACCCCGAGGG GTGCCCAGGG GCACACTCCT CCACCGCAGA GGTAGAAGAA
    GAGGAACCAG TGGAGGGGGA ACAAGAGCTC TGGGGCCCCG GGATGCCCCC GGGTGCCAAG
    TGGTACCCCG GGTCCGTGAG GCGAGCCACC TTGGAGTTTG AGGAGCGCCT GCGGCAAGAA
    CAGGAGCACC ATGGTGCTGC CCCTGCGGGG ACCTCGCTGT CCACTCGCAA GAATTCCAAG
    AACGATTCTT CAGTGGCAGA CCTGGCAGCG AAAGGGAAGA GTGACGAAGC CGCCCCAGAA
    CTGTCATATG TCCCCAAGGA GCCAGAGACG GGCAGGGGCC AAGGGCGATG CGGTGGGTCT
    GAGTCTGGCT CTTTACTGCG TCCTGAGCAG CTTGCCATCG TCCCGGCCCC TGAGCTGCGG
    GAGTCTCACC CAGCGCCAGC AACTCAGGAT GGCCCACGGC ATGAAGGTAT CCTGCGGGAG
    CCGAGGACTG TGGTCTCCGG CCAGGGGCCC GAGACACCAG CGGCCCCTGC TGCCTTTCCC
    AAGAAGATAG AAATCATTGA ATACACCTAC ACAGCCACAT CCCCTGATCC CGGGCCAGGG
    TGGGAAAGAG CCAGCAGTGA GAAGAGTGGG GCGCAGGGAC TGAGGACAGT GAAGGTGGAA
    GAGACCCTCA CTGTCCTCTG TGCTCTGGAT GAAAATCTGA ACCGGACGCT GAGCCCCGAC
    CAGGCTCCTC TGCACCCCAG AGTGCTACCT CCGCCTCATT CTTCCTCTCC CCAGCATGAG
    CACAGCAGGC CAGTCGACCT GCCCCCCACC CTGAGCAGCC CAGCAGCGCC TATTGTCAGT
    CCCACGACCC AGCCATGTGG CGCCAGCCCG GATCACCTGC ACCCCCAGAC GGTGGTTCAC
    CTGGAAGGCT TCACAGGGCA GAGCAGCACC ACAGACAGCG AGCCCTCCAC GGAGCAGGGC
    AGCTGGGAAG AAAGTCAGGA GGGCCCCCTC TCCAGGGCCA GTGAAGTGCC ATATCAGGGC
    TCCCAGTTCA GTAGCGAAGA CCTGCATTTA ATCAGCCAAC TGGGTAATAA TATCGGGGAG
    CAACCAGAAA AACTGGACCC AACATCTGTA GCCCATCAGC TCCCACACAG CTCCAGTAGC
    GACAGTATAG AGAGTCCCAG TCAGAGCCCC AGGGTGGTGA AGGGACGCGC TAAAGAAATC
    GAGTCCCGAG CAACTTCCCA GGTAGGGCTC CCCAAGCCAC CCCAAATGAG GCGTTCAGCT
    TCCCTTGCCA AGTTAGGTTA CTTGGACCTC TGTAAAGACT GTTCACCAGA GAGGGAGCCT
    GTCTCCTCTG AGTCCCCTCA TCTCAAACTG CTTCAGCCCT TCCTCAGAAC GGACTCGGGC
    ATGGAGGCCC AGGAGCCCCC AGAAAACCCA GACACTCCCC AGAACCGAGA GCCCACCAAG
    TATTTGATAG AGCAGCTCAG AACCACAGAG TGCATCGCGC AGAGCAGGCC AGTGGAGAGG
    CCCCTTGCAC AGTATGCCAA AGAGTTCGGT TCCAGTCAGC AGTGTTTGCT CCCCAGGGCA
    GGACCTGGAT TGACTAGTTC GGAAGGAGGC CTTCCTTCGG TACCGACCCA GGGACTGCAG
    GACACCAGCC CGGCCCCGGG GCTGGCTGTG GCGCCCCGAG AGCAGCACGG CAGAACTCAC
    CCCCTTAGGA GACTGAGAAA AGCAAATGAT AAAAAACGGA CAACCAACCC CTTCTATAAT
    ACCATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29562
    Clone ID Related Accession (Same CDS sequence) XM_005195614.3 , XM_005220148.3
    Accession Version XM_005195614.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005195614.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGCTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCC GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CGAAAGAGAT ACGAACTGAA CTAGAAATGC AGATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCACGT GTTCTTGGGC TCAGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAGATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAATTCATC TCTAAAGCAA AGAAACATGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTGATCGC CTATGCGATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCA
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAA CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GACCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAC CCATCCCTCC CGTCTTTGTG
    GAACATGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACATCTCA GGAATTTCAC GCCGGACAGA TTGAGGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAAGG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCGGAT
    CCTGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTGGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAA TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCGATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTTGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGCCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CGCCGAGAAC AGACCTGGAA ACCTGGAGCA GAGTGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGGGACCC CGAGGGGTGC
    CCAGGGGCAC ACTCCTCCAC CGCAGAGGTA GAAGAAGAGG AACCAGTGGA GGGGGAACAA
    GAGCTCTGGG GCCCCGGGAT GCCCCCGGGT GCCAAGTGGT ACCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGACCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCAGT GGCAGACCTG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACTGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAG CTGCGGGAGT CTCACCCAGC GCCAGCAACT
    CAGGATGGCC CACGGCATGA AGGTATCCTG CGGGAGCCGA GGACTGTGGT CTCCGGCCAG
    GGGCCCGAGA CACCAGCGGC CCCTGCTGCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGCGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCCCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CATGAGCACA GCAGGCCAGT CGACCTGCCC
    CCCACCCTGA GCAGCCCAGC AGCGCCTATT GTCAGTCCCA CGACCCAGCC ATGTGGCGCC
    AGCCCGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCAACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTCCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC ACCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAACGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TGATAGAGCA GCTCAGAACC
    ACAGAGTGCA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGCACAGTA TGCCAAAGAG
    TTCGGTTCCA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGGATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTCGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCGGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005195671.2
    CDS1028..5152
    Translation

    Target ORF information:

    RefSeq Version XM_005195614.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005195614.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGCTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCC GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CGAAAGAGAT ACGAACTGAA CTAGAAATGC AGATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCACGT GTTCTTGGGC TCAGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAGATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAATTCATC TCTAAAGCAA AGAAACATGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTGATCGC CTATGCGATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCA
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAA CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GACCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAC CCATCCCTCC CGTCTTTGTG
    GAACATGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACATCTCA GGAATTTCAC GCCGGACAGA TTGAGGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAAGG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCGGAT
    CCTGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTGGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAA TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCGATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTTGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGCCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CGCCGAGAAC AGACCTGGAA ACCTGGAGCA GAGTGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGGGACCC CGAGGGGTGC
    CCAGGGGCAC ACTCCTCCAC CGCAGAGGTA GAAGAAGAGG AACCAGTGGA GGGGGAACAA
    GAGCTCTGGG GCCCCGGGAT GCCCCCGGGT GCCAAGTGGT ACCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGACCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCAGT GGCAGACCTG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACTGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAG CTGCGGGAGT CTCACCCAGC GCCAGCAACT
    CAGGATGGCC CACGGCATGA AGGTATCCTG CGGGAGCCGA GGACTGTGGT CTCCGGCCAG
    GGGCCCGAGA CACCAGCGGC CCCTGCTGCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGCGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCCCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CATGAGCACA GCAGGCCAGT CGACCTGCCC
    CCCACCCTGA GCAGCCCAGC AGCGCCTATT GTCAGTCCCA CGACCCAGCC ATGTGGCGCC
    AGCCCGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCAACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTCCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC ACCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAACGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TGATAGAGCA GCTCAGAACC
    ACAGAGTGCA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGCACAGTA TGCCAAAGAG
    TTCGGTTCCA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGGATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTCGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCGGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa29562
    Clone ID Related Accession (Same CDS sequence) XM_005195614.3 , XM_005220148.3
    Accession Version XM_005220148.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4125bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005220148.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGCTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCC GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CGAAAGAGAT ACGAACTGAA CTAGAAATGC AGATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCACGT GTTCTTGGGC TCAGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAGATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAATTCATC TCTAAAGCAA AGAAACATGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTGATCGC CTATGCGATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCA
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAA CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GACCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAC CCATCCCTCC CGTCTTTGTG
    GAACATGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACATCTCA GGAATTTCAC GCCGGACAGA TTGAGGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAAGG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCGGAT
    CCTGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTGGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAA TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCGATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTTGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGCCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CGCCGAGAAC AGACCTGGAA ACCTGGAGCA GAGTGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGGGACCC CGAGGGGTGC
    CCAGGGGCAC ACTCCTCCAC CGCAGAGGTA GAAGAAGAGG AACCAGTGGA GGGGGAACAA
    GAGCTCTGGG GCCCCGGGAT GCCCCCGGGT GCCAAGTGGT ACCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGACCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCAGT GGCAGACCTG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACTGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAG CTGCGGGAGT CTCACCCAGC GCCAGCAACT
    CAGGATGGCC CACGGCATGA AGGTATCCTG CGGGAGCCGA GGACTGTGGT CTCCGGCCAG
    GGGCCCGAGA CACCAGCGGC CCCTGCTGCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGCGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCCCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CATGAGCACA GCAGGCCAGT CGACCTGCCC
    CCCACCCTGA GCAGCCCAGC AGCGCCTATT GTCAGTCCCA CGACCCAGCC ATGTGGCGCC
    AGCCCGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCAACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTCCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC ACCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAACGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TGATAGAGCA GCTCAGAACC
    ACAGAGTGCA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGCACAGTA TGCCAAAGAG
    TTCGGTTCCA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGGATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTCGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCGGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005220205.1
    CDS1028..5152
    Translation

    Target ORF information:

    RefSeq Version XM_005220148.3
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005220148.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAAACGTC AGATGAGCTT CAGCATCAGC GAGAGCTTTC TAACTGTCAA AGGTGCTGCC 
    CTTTTTCTAC CCCGGGGAAA TGGCTCATCC ACACCAAGAG TCAGCCACAG ACGCAACAAG
    CATGCAGGTG ATCTCCAACA GCATCTCCAA GCAATGTTCA TACTACTTCG CCCAGAAGAC
    AACATCAGGC TGGCTGTAAG ACTAGAAAGT ACTTACCAGA ATCGAACACG CTATATGGTA
    GTGGTTTCAA CTAATGGTAG ACAAGACACT GAAGAAAGCA TCGTCCTAGG AATGGATTTC
    TCCTCTAATG ACAGTAGCAC TTGTACCATG GGCTTAGTCC TGCCTCTCTG GAGTGACACC
    CTAATCCATT TGGATGGTGA TGGTGGGTTC AGCGTCTCGA CAGATAACAG AGTTCACATA
    TTCAAACCTG TATCTGTGCA GGCAATGTGG TCTGCACTGC AGAGTTTACA CAAGGCTTGT
    GAAGTGGCCA GAATGCATAA CTACTACCCC GGCAGCCTGT TTCTCACCTG GGTGAGTTAC
    TATGAGAGCC ATATCAACTC AGACCAGTCC TCGGTCAACG AATGGAATGC TATGCAGGAT
    GTACAGTCCC ACCGACCTGA CTCTCCTGCT CTCTTCACAG ACATACCAAC TGAACGTGAG
    CGGACAGAGA GGCTAATTAA AACCAGATTA AGGGAGATTA TGATGCAAAA GGATTTGGAG
    AATATCACAT CGAAAGAGAT ACGAACTGAA CTAGAAATGC AGATGGTGTG CAACTTGCGA
    GAATTCAAGG AATTTATAGA TAATGAAATG ATAGTGATCC TTGGTCAGAT GGATAGCCCT
    ACACAGATAT TCGAGCACGT GTTCTTGGGC TCAGAATGGA ATGCCTCCAA CTTAGAGGAT
    TTACAGAACC GAGGGGTTCG CTATATCTTG AATGTCACTC GAGAGATAGA CAACTTCTTC
    CCGGGAGTCT TTGAGTATCA CAACATTCGG GTGTATGATG AAGAGGCAAC CGACCTCCTG
    GCTTACTGGA ATGACACTTA CAAATTCATC TCTAAAGCAA AGAAACATGG ATCTAAATGC
    CTCGTGCACT GCAAAATGGG GGTGAGTCGC TCGGCCTCCA CTGTGATCGC CTATGCGATG
    AAGGAGTATG GCTGGAACTT GGACCGAGCC TATGACTATG TGAAGGAAAG ACGGACAGTG
    ACCAAGCCCA ACCCCAGCTT CATGAGACAG CTGGAAGAGT ACCAGGGGAT CTTGCTGGCA
    AGCAAACAGC GGCACAACAA ACTCTGGAGA TCGCATTCAG ATAGCGACCT CTCGGACCAC
    CACGAACCCA TCTGCAAACC AGGACTGGAA CTCAACAAAA AGGAGATTAC CACCTCAGCA
    GACCAGATTG CCGAGGTGAA GACCATGGAG AGCCACCCAC CCATCCCTCC CGTCTTTGTG
    GAACATGTGG TCCCGCAGGA TGAGAATCAG AAAGCCCTGT GCACCAAAGA AAGAATGATC
    TGCTTGGAGT TTACATCTCA GGAATTTCAC GCCGGACAGA TTGAGGATGA ATTAAACCTA
    AATGACATCA ATGGATGCTC ATCAGGGTGT TGTCTCAATG AACCGAAATT CCCTCTTGAC
    AACTGCCATG CATCCAAAGC CTTAATCCAA CCTGGAAAGG ACCCAGAAAT GGCCCACAAG
    TTCCCAGACT TAACTGTGGA AGATCTGGAG ACGGACGCGC TGAAAGCAGA CATGAATGTC
    CACCTGCTGC CCATGGAAGA GCTGACATCC CGCCTGAAAG ATCTCCCCAT GTCCCCGGAT
    CCTGAGTCAC CAAGCCCCCA ACCCAGCTGC CAGGCCACAG TCTCAGATTT CAGTGGAGAT
    CGCATTGACT TTTTCAGCGC TCTGGAGAAG TTTGTAGAGC TTTCCCAAGA AACCCGGTCC
    CGATCTTTTT CTCATTCAAG GATGGAGGAA GTGGGTGGAG GAAGGAATGA GAGCTGTCGA
    CTGTCAGTGG TGGAAGCAGC CCCTTCTGAA GCGACCACTG ATGACCAGAG AAGCAGCTCT
    TTGAGTAATA CTCCCCACGC ATCTGAGGAA TCTTCAGTAG ATGAGGAGCA GTCAAAGGCA
    ATCTCAGAAC AGGTCAGCCC GGACATCTTC ATGCAGCCGC ACTCAGAAAA TGCGATGTCA
    GTCAAAGAAA TCGTCACTGA GATTGAATCC ATCAGTCAAG GAGTTGGACA GATTCAAGTG
    AAAGGAGACG TGCTGTCCAA CCCATGCCAC ACACCAAAGA AGCACATCGT CCACGAGCTG
    CCCCTGGAGA GGGCCCAGGC CGCCGAGAAC AGACCTGGAA ACCTGGAGCA GAGTGAAGGT
    TCCTGCCCAG CCCAGCCTGA ACCAGCCAAA GACTCAGGGA AGGGGGACCC CGAGGGGTGC
    CCAGGGGCAC ACTCCTCCAC CGCAGAGGTA GAAGAAGAGG AACCAGTGGA GGGGGAACAA
    GAGCTCTGGG GCCCCGGGAT GCCCCCGGGT GCCAAGTGGT ACCCCGGGTC CGTGAGGCGA
    GCCACCTTGG AGTTTGAGGA GCGCCTGCGG CAAGAACAGG AGCACCATGG TGCTGCCCCT
    GCGGGGACCT CGCTGTCCAC TCGCAAGAAT TCCAAGAACG ATTCTTCAGT GGCAGACCTG
    GCAGCGAAAG GGAAGAGTGA CGAAGCCGCC CCAGAACTGT CATATGTCCC CAAGGAGCCA
    GAGACGGGCA GGGGCCAAGG GCGATGCGGT GGGTCTGAGT CTGGCTCTTT ACTGCGTCCT
    GAGCAGCTTG CCATCGTCCC GGCCCCTGAG CTGCGGGAGT CTCACCCAGC GCCAGCAACT
    CAGGATGGCC CACGGCATGA AGGTATCCTG CGGGAGCCGA GGACTGTGGT CTCCGGCCAG
    GGGCCCGAGA CACCAGCGGC CCCTGCTGCC TTTCCCAAGA AGATAGAAAT CATTGAATAC
    ACCTACACAG CCACATCCCC TGATCCCGGG CCAGGGTGGG AAAGAGCCAG CAGTGAGAAG
    AGTGGGGCGC AGGGACTGAG GACAGTGAAG GTGGAAGAGA CCCTCACTGT CCTCTGTGCT
    CTGGATGAAA ATCTGAACCG GACGCTGAGC CCCGACCAGG CTCCTCTGCA CCCCAGAGTG
    CTACCTCCGC CTCATTCTTC CTCTCCCCAG CATGAGCACA GCAGGCCAGT CGACCTGCCC
    CCCACCCTGA GCAGCCCAGC AGCGCCTATT GTCAGTCCCA CGACCCAGCC ATGTGGCGCC
    AGCCCGGATC ACCTGCACCC CCAGACGGTG GTTCACCTGG AAGGCTTCAC AGGGCAGAGC
    AGCACCACAG ACAGCGAGCC CTCCACGGAG CAGGGCAGCT GGGAAGAAAG TCAGGAGGGC
    CCCCTCTCCA GGGCCAGTGA AGTGCCATAT CAGGGCTCCC AGTTCAGTAG CGAAGACCTG
    CATTTAATCA GCCAACTGGG TAATAATATC GGGGAGCAAC CAGAAAAACT GGACCCAACA
    TCTGTAGCCC ATCAGCTCCC ACACAGCTCC AGTAGCGACA GTATAGAGAG TCCCAGTCAG
    AGCCCCAGGG TGGTGAAGGG ACGCGCTAAA GAAATCGAGT CCCGAGCAAC TTCCCAGGTA
    GGGCTCCCCA AGCCACCCCA AATGAGGCGT TCAGCTTCCC TTGCCAAGTT AGGTTACTTG
    GACCTCTGTA AAGACTGTTC ACCAGAGAGG GAGCCTGTCT CCTCTGAGTC CCCTCATCTC
    AAACTGCTTC AGCCCTTCCT CAGAACGGAC TCGGGCATGG AGGCCCAGGA GCCCCCAGAA
    AACCCAGACA CTCCCCAGAA CCGAGAGCCC ACCAAGTATT TGATAGAGCA GCTCAGAACC
    ACAGAGTGCA TCGCGCAGAG CAGGCCAGTG GAGAGGCCCC TTGCACAGTA TGCCAAAGAG
    TTCGGTTCCA GTCAGCAGTG TTTGCTCCCC AGGGCAGGAC CTGGATTGAC TAGTTCGGAA
    GGAGGCCTTC CTTCGGTACC GACCCAGGGA CTGCAGGACA CCAGCCCGGC CCCGGGGCTG
    GCTGTGGCGC CCCGAGAGCA GCACGGCAGA ACTCACCCCC TTAGGAGACT GAGAAAAGCA
    AATGATAAAA AACGGACAAC CAACCCCTTC TATAATACCA TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48385
    Clone ID Related Accession (Same CDS sequence) XM_010815867.2 , XM_010824015.2
    Accession Version XM_010815867.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104492.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010815867.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACATCA GCGAGAGCTT TCTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCCCGGGGA 
    AATGGCTCAT CCACACCAAG AGTCAGCCAC AGACGCAACA AGCATGCAGG TGATCTCCAA
    CAGCATCTCC AAGCAATGTT CATACTACTT CGCCCAGAAG ACAACATCAG GCTGGCTGTA
    AGACTAGAAA GTACTTACCA GAATCGAACA CGCTATATGG TAGTGGTTTC AACTAATGGT
    AGACAAGACA CTGAAGAAAG CATCGTCCTA GGAATGGATT TCTCCTCTAA TGACAGTAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010814169.1
    CDS1884..5990
    Translation

    Target ORF information:

    RefSeq Version XM_010815867.2
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010815867.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACATCA GCGAGAGCTT TCTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCCCGGGGA 
    AATGGCTCAT CCACACCAAG AGTCAGCCAC AGACGCAACA AGCATGCAGG TGATCTCCAA
    CAGCATCTCC AAGCAATGTT CATACTACTT CGCCCAGAAG ACAACATCAG GCTGGCTGTA
    AGACTAGAAA GTACTTACCA GAATCGAACA CGCTATATGG TAGTGGTTTC AACTAATGGT
    AGACAAGACA CTGAAGAAAG CATCGTCCTA GGAATGGATT TCTCCTCTAA TGACAGTAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48385
    Clone ID Related Accession (Same CDS sequence) XM_010815867.2 , XM_010824015.2
    Accession Version XM_010824015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4107bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product protein phosphatase Slingshot homolog 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646451.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACATCA GCGAGAGCTT TCTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCCCGGGGA 
    AATGGCTCAT CCACACCAAG AGTCAGCCAC AGACGCAACA AGCATGCAGG TGATCTCCAA
    CAGCATCTCC AAGCAATGTT CATACTACTT CGCCCAGAAG ACAACATCAG GCTGGCTGTA
    AGACTAGAAA GTACTTACCA GAATCGAACA CGCTATATGG TAGTGGTTTC AACTAATGGT
    AGACAAGACA CTGAAGAAAG CATCGTCCTA GGAATGGATT TCTCCTCTAA TGACAGTAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822317.2
    CDS1884..5990
    Translation

    Target ORF information:

    RefSeq Version XM_010824015.2
    Organism Bos taurus(cattle)
    Definition Bos taurus slingshot protein phosphatase 2 (SSH2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAACATCA GCGAGAGCTT TCTAACTGTC AAAGGTGCTG CCCTTTTTCT ACCCCGGGGA 
    AATGGCTCAT CCACACCAAG AGTCAGCCAC AGACGCAACA AGCATGCAGG TGATCTCCAA
    CAGCATCTCC AAGCAATGTT CATACTACTT CGCCCAGAAG ACAACATCAG GCTGGCTGTA
    AGACTAGAAA GTACTTACCA GAATCGAACA CGCTATATGG TAGTGGTTTC AACTAATGGT
    AGACAAGACA CTGAAGAAAG CATCGTCCTA GGAATGGATT TCTCCTCTAA TGACAGTAGC
    ACTTGTACCA TGGGCTTAGT CCTGCCTCTC TGGAGTGACA CCCTAATCCA TTTGGATGGT
    GATGGTGGGT TCAGCGTCTC GACAGATAAC AGAGTTCACA TATTCAAACC TGTATCTGTG
    CAGGCAATGT GGTCTGCACT GCAGAGTTTA CACAAGGCTT GTGAAGTGGC CAGAATGCAT
    AACTACTACC CCGGCAGCCT GTTTCTCACC TGGGTGAGTT ACTATGAGAG CCATATCAAC
    TCAGACCAGT CCTCGGTCAA CGAATGGAAT GCTATGCAGG ATGTACAGTC CCACCGACCT
    GACTCTCCTG CTCTCTTCAC AGACATACCA ACTGAACGTG AGCGGACAGA GAGGCTAATT
    AAAACCAGAT TAAGGGAGAT TATGATGCAA AAGGATTTGG AGAATATCAC ATCGAAAGAG
    ATACGAACTG AACTAGAAAT GCAGATGGTG TGCAACTTGC GAGAATTCAA GGAATTTATA
    GATAATGAAA TGATAGTGAT CCTTGGTCAG ATGGATAGCC CTACACAGAT ATTCGAGCAC
    GTGTTCTTGG GCTCAGAATG GAATGCCTCC AACTTAGAGG ATTTACAGAA CCGAGGGGTT
    CGCTATATCT TGAATGTCAC TCGAGAGATA GACAACTTCT TCCCGGGAGT CTTTGAGTAT
    CACAACATTC GGGTGTATGA TGAAGAGGCA ACCGACCTCC TGGCTTACTG GAATGACACT
    TACAAATTCA TCTCTAAAGC AAAGAAACAT GGATCTAAAT GCCTCGTGCA CTGCAAAATG
    GGGGTGAGTC GCTCGGCCTC CACTGTGATC GCCTATGCGA TGAAGGAGTA TGGCTGGAAC
    TTGGACCGAG CCTATGACTA TGTGAAGGAA AGACGGACAG TGACCAAGCC CAACCCCAGC
    TTCATGAGAC AGCTGGAAGA GTACCAGGGG ATCTTGCTGG CAAGCAAACA GCGGCACAAC
    AAACTCTGGA GATCGCATTC AGATAGCGAC CTCTCGGACC ACCACGAACC CATCTGCAAA
    CCAGGACTGG AACTCAACAA AAAGGAGATT ACCACCTCAG CAGACCAGAT TGCCGAGGTG
    AAGACCATGG AGAGCCACCC ACCCATCCCT CCCGTCTTTG TGGAACATGT GGTCCCGCAG
    GATGAGAATC AGAAAGCCCT GTGCACCAAA GAAAGAATGA TCTGCTTGGA GTTTACATCT
    CAGGAATTTC ACGCCGGACA GATTGAGGAT GAATTAAACC TAAATGACAT CAATGGATGC
    TCATCAGGGT GTTGTCTCAA TGAACCGAAA TTCCCTCTTG ACAACTGCCA TGCATCCAAA
    GCCTTAATCC AACCTGGAAA GGACCCAGAA ATGGCCCACA AGTTCCCAGA CTTAACTGTG
    GAAGATCTGG AGACGGACGC GCTGAAAGCA GACATGAATG TCCACCTGCT GCCCATGGAA
    GAGCTGACAT CCCGCCTGAA AGATCTCCCC ATGTCCCCGG ATCCTGAGTC ACCAAGCCCC
    CAACCCAGCT GCCAGGCCAC AGTCTCAGAT TTCAGTGGAG ATCGCATTGA CTTTTTCAGC
    GCTCTGGAGA AGTTTGTAGA GCTTTCCCAA GAAACCCGGT CCCGATCTTT TTCTCATTCA
    AGGATGGAGG AAGTGGGTGG AGGAAGGAAT GAGAGCTGTC GACTGTCAGT GGTGGAAGCA
    GCCCCTTCTG AAGCGACCAC TGATGACCAG AGAAGCAGCT CTTTGAGTAA TACTCCCCAC
    GCATCTGAGG AATCTTCAGT AGATGAGGAG CAGTCAAAGG CAATCTCAGA ACAGGTCAGC
    CCGGACATCT TCATGCAGCC GCACTCAGAA AATGCGATGT CAGTCAAAGA AATCGTCACT
    GAGATTGAAT CCATCAGTCA AGGAGTTGGA CAGATTCAAG TGAAAGGAGA CGTGCTGTCC
    AACCCATGCC ACACACCAAA GAAGCACATC GTCCACGAGC TGCCCCTGGA GAGGGCCCAG
    GCCGCCGAGA ACAGACCTGG AAACCTGGAG CAGAGTGAAG GTTCCTGCCC AGCCCAGCCT
    GAACCAGCCA AAGACTCAGG GAAGGGGGAC CCCGAGGGGT GCCCAGGGGC ACACTCCTCC
    ACCGCAGAGG TAGAAGAAGA GGAACCAGTG GAGGGGGAAC AAGAGCTCTG GGGCCCCGGG
    ATGCCCCCGG GTGCCAAGTG GTACCCCGGG TCCGTGAGGC GAGCCACCTT GGAGTTTGAG
    GAGCGCCTGC GGCAAGAACA GGAGCACCAT GGTGCTGCCC CTGCGGGGAC CTCGCTGTCC
    ACTCGCAAGA ATTCCAAGAA CGATTCTTCA GTGGCAGACC TGGCAGCGAA AGGGAAGAGT
    GACGAAGCCG CCCCAGAACT GTCATATGTC CCCAAGGAGC CAGAGACGGG CAGGGGCCAA
    GGGCGATGCG GTGGGTCTGA GTCTGGCTCT TTACTGCGTC CTGAGCAGCT TGCCATCGTC
    CCGGCCCCTG AGCTGCGGGA GTCTCACCCA GCGCCAGCAA CTCAGGATGG CCCACGGCAT
    GAAGGTATCC TGCGGGAGCC GAGGACTGTG GTCTCCGGCC AGGGGCCCGA GACACCAGCG
    GCCCCTGCTG CCTTTCCCAA GAAGATAGAA ATCATTGAAT ACACCTACAC AGCCACATCC
    CCTGATCCCG GGCCAGGGTG GGAAAGAGCC AGCAGTGAGA AGAGTGGGGC GCAGGGACTG
    AGGACAGTGA AGGTGGAAGA GACCCTCACT GTCCTCTGTG CTCTGGATGA AAATCTGAAC
    CGGACGCTGA GCCCCGACCA GGCTCCTCTG CACCCCAGAG TGCTACCTCC GCCTCATTCT
    TCCTCTCCCC AGCATGAGCA CAGCAGGCCA GTCGACCTGC CCCCCACCCT GAGCAGCCCA
    GCAGCGCCTA TTGTCAGTCC CACGACCCAG CCATGTGGCG CCAGCCCGGA TCACCTGCAC
    CCCCAGACGG TGGTTCACCT GGAAGGCTTC ACAGGGCAGA GCAGCACCAC AGACAGCGAG
    CCCTCCACGG AGCAGGGCAG CTGGGAAGAA AGTCAGGAGG GCCCCCTCTC CAGGGCCAGT
    GAAGTGCCAT ATCAGGGCTC CCAGTTCAGT AGCGAAGACC TGCATTTAAT CAGCCAACTG
    GGTAATAATA TCGGGGAGCA ACCAGAAAAA CTGGACCCAA CATCTGTAGC CCATCAGCTC
    CCACACAGCT CCAGTAGCGA CAGTATAGAG AGTCCCAGTC AGAGCCCCAG GGTGGTGAAG
    GGACGCGCTA AAGAAATCGA GTCCCGAGCA ACTTCCCAGG TAGGGCTCCC CAAGCCACCC
    CAAATGAGGC GTTCAGCTTC CCTTGCCAAG TTAGGTTACT TGGACCTCTG TAAAGACTGT
    TCACCAGAGA GGGAGCCTGT CTCCTCTGAG TCCCCTCATC TCAAACTGCT TCAGCCCTTC
    CTCAGAACGG ACTCGGGCAT GGAGGCCCAG GAGCCCCCAG AAAACCCAGA CACTCCCCAG
    AACCGAGAGC CCACCAAGTA TTTGATAGAG CAGCTCAGAA CCACAGAGTG CATCGCGCAG
    AGCAGGCCAG TGGAGAGGCC CCTTGCACAG TATGCCAAAG AGTTCGGTTC CAGTCAGCAG
    TGTTTGCTCC CCAGGGCAGG ACCTGGATTG ACTAGTTCGG AAGGAGGCCT TCCTTCGGTA
    CCGACCCAGG GACTGCAGGA CACCAGCCCG GCCCCGGGGC TGGCTGTGGC GCCCCGAGAG
    CAGCACGGCA GAACTCACCC CCTTAGGAGA CTGAGAAAAG CAAATGATAA AAAACGGACA
    ACCAACCCCT TCTATAATAC CATGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.