ADGRF1 cDNA ORF clone, Bos taurus(cattle)

The following ADGRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ADGRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa31991 NM_001192327.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa31991 XM_010818181.3
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa31991 XM_010818180.2
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa31991 XM_024983555.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa31991 XM_024983554.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48378 XM_010818183.2
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48379 XM_024983556.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48379 XM_010818184.3
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48379 XM_024983557.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa229470 XM_024983550.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa229470 XM_024983553.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa229470 XM_024983552.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa229470 XM_024983551.1
Latest version!
Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OBa31991 XM_010818181.2 Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa48379 XM_010818184.2 Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version NM_001192327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000023.1. On Jul 17, 2010 this sequence version replaced XM_590184.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179256.1
    CDS174..3002
    Translation

    Target ORF information:

    RefSeq Version NM_001192327.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version XM_010818181.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816483.1
    CDS285..3113
    Translation

    Target ORF information:

    RefSeq Version XM_010818181.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818181.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version XM_010818180.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816482.1
    CDS1550..4378
    Translation

    Target ORF information:

    RefSeq Version XM_010818180.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818180.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version XM_010818181.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816483.1
    CDS189..3017
    Translation

    Target ORF information:

    RefSeq Version XM_010818181.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818181.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version XM_024983554.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839322.1
    CDS329..3157
    Translation

    Target ORF information:

    RefSeq Version XM_024983554.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983554.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31991
    Clone ID Related Accession (Same CDS sequence) XM_010818181.2 , XM_010818180.2 , XM_010818181.3 , XM_024983554.1 , NM_001192327.1 , XM_024983555.1
    Accession Version XM_024983555.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839323.1
    CDS70..2898
    Translation

    Target ORF information:

    RefSeq Version XM_024983555.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983555.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48378
    Clone ID Related Accession (Same CDS sequence) XM_010818183.2
    Accession Version XM_010818183.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818183.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGAAGTTAT CAGATTCATC TGTCACACTC
    AGATTCGAAA AGCCTTTCAA CCCATTCCAG CAGAAAGGCT GCTATGTATT TTCATATACT
    AGCGAATCCT CACATGACAT CATGCTAACT CAGTTTTCAT CAGCTGAACA AAGCGGGGAA
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816485.1
    CDS267..3092
    Translation

    Target ORF information:

    RefSeq Version XM_010818183.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818183.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC 
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGAAGTTAT CAGATTCATC TGTCACACTC
    AGATTCGAAA AGCCTTTCAA CCCATTCCAG CAGAAAGGCT GCTATGTATT TTCATATACT
    AGCGAATCCT CACATGACAT CATGCTAACT CAGTTTTCAT CAGCTGAACA AAGCGGGGAA
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48379
    Clone ID Related Accession (Same CDS sequence) XM_010818184.3 , XM_024983556.1 , XM_010818184.2 , XM_024983557.1
    Accession Version XM_010818184.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010818184.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816486.1
    CDS322..3060
    Translation

    Target ORF information:

    RefSeq Version XM_010818184.3
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818184.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48379
    Clone ID Related Accession (Same CDS sequence) XM_010818184.3 , XM_024983556.1 , XM_010818184.2 , XM_024983557.1
    Accession Version XM_024983556.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839324.1
    CDS137..2875
    Translation

    Target ORF information:

    RefSeq Version XM_024983556.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983556.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48379
    Clone ID Related Accession (Same CDS sequence) XM_010818184.3 , XM_024983556.1 , XM_010818184.2 , XM_024983557.1
    Accession Version XM_010818184.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104551.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010818184.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010816486.1
    CDS319..3057
    Translation

    Target ORF information:

    RefSeq Version XM_010818184.2
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010818184.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48379
    Clone ID Related Accession (Same CDS sequence) XM_010818184.3 , XM_024983556.1 , XM_010818184.2 , XM_024983557.1
    Accession Version XM_024983557.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2739bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839325.1
    CDS123..2861
    Translation

    Target ORF information:

    RefSeq Version XM_024983557.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983557.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGAGATTTC TTCCTCTCTG GCTCTTCTCC TTCCTCACGA TCACCGAGGG CCGAGATGGC 
    TTCCTAGAGA GGGATGAAGG CATCAAAACA AAACAAGAAC TCATTGTGAA TAAGGAAAAG
    CCTCTAGGCT CAATCCAGGA ATATGAGCTG CTGCTTCAGG TGCACTATCG AAATTCCAAG
    GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG TTCTGTGCGC TTTCCTCATT TTTCTTACCT
    GAGCCAGTGA AGATAATCAG AGCAAAGGCC ACCACGTACT GTGGCTACCA GAACAGGGCC
    CTGCGCTGTG CCTGTGAAGA CAGCTACTCC TGGTTTCCTC CCCAGTGCCT CGATCCCCAG
    AACTGCTCCC TTCTCAAGGC AGGATCACCC CAGACCTGTG ACTGTCATTC CAGCAACCTC
    ACCCAGAGCG TCTATTTCTG TGAGAGAACA AAGGTTTGGG GTACTTTCAA AATTAATAAG
    AAATTTACAG AAGACCTTTT GGACTCATCT TCTGCCAAGT ACGCCAAATA TACCACTGGA
    ATTGAAATGC AGCTAAAGGA AGCATACAAA GGAATCCAAG GTTTTGAATC TGTTCGAGTC
    ATCCAGTTTC GAGATGGAAG CATCGTTGTT GGGTATGAAG TTGTTGGTTC CAGTGGCACA
    TCTGAGCTCC TGTCGGGCAT TGAGCAGATG GTTGAGAAAG CTAAGACATA TCTGCGCCAG
    CTGTATACAC TTGAAGAAAA TTCTTTCAGA GTGTTCGGAG AAGCCCAGTG TAACAGCATT
    GCCTTTGGAT TTGGGTCTGA GAATGACGAG TACACTCTTC CCTGCAGCCT CGGCTACATC
    GGAAACATCA CAGTCAGGTG CCAACCCTCT GGGTGGCATA TCCTTGAAGA GATGTGTGTG
    CTCTCTGAGC TGGAAGAACT GAAGAAGAAT TCACGTGAGC TGGCCCGCAA CAGTACTGAC
    GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG AAGCTTTCGA TGGTCATCCA GCAAAGCCCA
    TCAACCACAG CTGGGAACCT GGCTTCCGTG GTGTCAATTC TGGGCAATAT CTCATCTCTG
    AGAAAAAGCT TCACGGTGTC CAATTCAACA ATGGAGAATG TCATCAATAT AGCTGATCAC
    ATCCTTAATT CAGCTTCGCT AACCAACTGG ACAGTGTTAC TGAAGAAAGA AAAGAATGCC
    AGTTCCCAGT TACTAGAATC ACTGGAAAAC ATTAGCACTC TGGTACCTTC GACAGCTCTG
    CCTCTGCATT TTTCTCGAGA CTTCATTAAC TGGAAAGGGA TTCCAGTATC TGCAAATCTC
    AGCAATCAAG GTTACAATTA TGAAACTGAA TTCCCCCCCC AAAATACCCC CAGTCCCATC
    ACAGGCCATG TGTCTATTGG GCCAGGACAA TTCAAGAAGC ACCTTCCACA TACTATCATC
    AGCATGACCT CCTTGACCCT GGGGAACATT CTACCCATTG CTGAAAATAA AAATGCTCAG
    GTTAATGGGC CCTTGATATC CATTATTATC CCAAACTCTT CCATCCATGA AATTTCCCTG
    ACTTTTTCTA AGATGAAATT GAATCTGAGT CAACCTCAAT GTGTGTTTTG GGATTTCAGT
    CATTTGCAGT GGACAGATAC CGGCTGCCAC CTAGAGAATG AAACTGCAAA CTTGGTGACA
    TGCCTTTGTA CTCACCTGAC CTCCTACTCC GTGCTGATGT CCTCTATTGT CCCTCCTGCC
    ATCGTCCCCA TTGTGAAATG GATCACCTTT GTGGGACTGG GGGTCTCCAT CGGAAGTCTC
    ATCTTATGCC TGACCATCCA AGGTCTGTTT TGGAAGCAGG TCAACAAAAA CCAAACCTCA
    CACAGCCGTC ACATCTGCAT AGTGAACATA GCTCTGTGCC TCCTGATTGC AGACGTCTGG
    TTTATTGTTG CTGCCACGGT GGACACAAAC TCTGGCGTCT GCACAGCTGC CGTGTTCTTT
    GCACACCTTT TCTACCTCTC CTTGTTCTTC TGGATGCTTC TGCTCGGGTT CCTGCTGGCA
    TATCGGGTGA TCTTCGTGTT CCATCACATG GCCACACCTT TGATGGTGGC TGTCGGGTTC
    TGCTTGGGCT ACGGGTGCCC TCTCATTATA TCCATCGTCA CCTTTCTGGT CACGCAGCCC
    AGCGATGGCT ACAAAAGGAA GGATGCATGT TGGCTTAACT GGTCCGATGG GAGCAAGCCT
    CTCTTGGCCT TTGCGGTTCC TGCCCTGACT ATTGTGGCTG TAAATTTGGT GGTGGTGCTA
    TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT GTTGGAGAAA GGCTGGCCCA GGACAACAAG
    GCCACTGTCA AGCGCATAGG GAGGAGCCTC CTCGTCCTGA CCCCTCTTCT GGGGCTCACC
    TGGGGTTTCG GCATAGTAAC AATGGCGGAC GGCCAGAATC TGTCTTGGCA TGTTATTTTC
    GCATTTCTCA ATGCATTCCA GGGTCTTTTC ATCTTGTGTT TTGGAATTCT CTTGGATAGT
    AAGCTGCGAC AGCTGCTGCT CAACAAGTTG TCTCCTTTAA GCTGTTGGGA CCAGACATCA
    AAGCAGAAGT TATCAGATTC ATCTGTCACA CTCAGATTCG AAAAGCCTTT CAACCCATTC
    CAGCAGAAAG GCTGCTATGT ATTTTCATAT ACTAGCGAAT CCTCACATGA CATCATGCTA
    ACTCAGTTTT CATCAGCTGA ACAAAGCGGG GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229470
    Clone ID Related Accession (Same CDS sequence) XM_024983550.1 , XM_024983552.1 , XM_024983551.1 , XM_024983553.1
    Accession Version XM_024983550.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839318.1
    CDS2870..5818
    Translation

    Target ORF information:

    RefSeq Version XM_024983550.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983550.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229470
    Clone ID Related Accession (Same CDS sequence) XM_024983550.1 , XM_024983552.1 , XM_024983551.1 , XM_024983553.1
    Accession Version XM_024983552.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839320.1
    CDS2828..5776
    Translation

    Target ORF information:

    RefSeq Version XM_024983552.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983552.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229470
    Clone ID Related Accession (Same CDS sequence) XM_024983550.1 , XM_024983552.1 , XM_024983551.1 , XM_024983553.1
    Accession Version XM_024983551.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839319.1
    CDS2921..5869
    Translation

    Target ORF information:

    RefSeq Version XM_024983551.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229470
    Clone ID Related Accession (Same CDS sequence) XM_024983550.1 , XM_024983552.1 , XM_024983551.1 , XM_024983553.1
    Accession Version XM_024983553.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2949bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product adhesion G-protein coupled receptor F1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839321.1
    CDS227..3175
    Translation

    Target ORF information:

    RefSeq Version XM_024983553.1
    Organism Bos taurus(cattle)
    Definition Bos taurus adhesion G protein-coupled receptor F1 (ADGRF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983553.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTATGAAA GCCTGCTAGT CTATGTAATT CATCCATGCC AGAGACTAAA TCATGGGAAG 
    TTACCAAAGG AGATCACAGA GCAAAGCACA GGGCAGTGCT GTTGGGATGA CAAAGCGTGC
    ATGAAGTTCA AAGGGCACCC AGAGAGGAAG TCACAGACTG TGACTCCAGC TGTTTGTGTC
    CTGTCCAGGA CTAGGAGGGA CCAGCAGAAG ATGAGATTTC TTCCTCTCTG GCTCTTCTCC
    TTCCTCACGA TCACCGAGGG CCGAGATGGC TTCCTAGAGA GGGATGAAGG CATCAAAACA
    AAACAAGAAC TCATTGTGAA TAAGGAAAAG CCTCTAGGCT CAATCCAGGA ATATGAGCTG
    CTGCTTCAGG TGCACTATCG AAATTCCAAG GAGAAAAGAG AGCTGAAGGA ATTTCTGAAG
    TTCTGTGCGC TTTCCTCATT TTTCTTACCT GAGCCAGTGA AGATAATCAG AGCAAAGGCC
    ACCACGTACT GTGGCTACCA GAACAGGGCC CTGCGCTGTG CCTGTGAAGA CAGCTACTCC
    TGGTTTCCTC CCCAGTGCCT CGATCCCCAG AACTGCTCCC TTCTCAAGGC AGGATCACCC
    CAGACCTGTG ACTGTCATTC CAGCAACCTC ACCCAGAGCG TCTATTTCTG TGAGAGAACA
    AAGGTTTGGG GTACTTTCAA AATTAATAAG AAATTTACAG AAGACCTTTT GGACTCATCT
    TCTGCCAAGT ACGCCAAATA TACCACTGGA ATTGAAATGC AGCTAAAGGA AGCATACAAA
    GGAATCCAAG GTTTTGAATC TGTTCGAGTC ATCCAGTTTC GAGATGGAAG CATCGTTGTT
    GGGTATGAAG TTGTTGGTTC CAGTGGCACA TCTGAGCTCC TGTCGGGCAT TGAGCAGATG
    GTTGAGAAAG CTAAGACATA TCTGCGCCAG CTGTATACAC TTGAAGAAAA TTCTTTCAGA
    GTGTTCGGAG AAGCCCAGTG TAACAGCATT GCCTTTGGAT TTGGGTCTGA GAATGACGAG
    TACACTCTTC CCTGCAGCCT CGGCTACATC GGAAACATCA CAGTCAGGTG CCAACCCTCT
    GGGTGGCATA TCCTTGAAGA GATGTGTGTG CTCTCTGAGC TGGAAGAACT GAAGAAGAAT
    TCACGTGAGC TGGCCCGCAA CAGTACTGAC GCAGAGGCAG CTGTGTCGTC TCTGGTGCAG
    AAGCTTTCGA TGGTCATCCA GCAAAGCCCA TCAACCACAG CTGGGAACCT GGCTTCCGTG
    GTGTCAATTC TGGGCAATAT CTCATCTCTG AGAAAAAGCT TCACGGTGTC CAATTCAACA
    ATGGAGAATG TCATCAATAT AGCTGATCAC ATCCTTAATT CAGCTTCGCT AACCAACTGG
    ACAGTGTTAC TGAAGAAAGA AAAGAATGCC AGTTCCCAGT TACTAGAATC ACTGGAAAAC
    ATTAGCACTC TGGTACCTTC GACAGCTCTG CCTCTGCATT TTTCTCGAGA CTTCATTAAC
    TGGAAAGGGA TTCCAGTATC TGCAAATCTC AGCAATCAAG GTTACAATTA TGAAACTGAA
    TTCCCCCCCC AAAATACCCC CAGTCCCATC ACAGGCCATG TGTCTATTGG GCCAGGACAA
    TTCAAGAAGC ACCTTCCACA TACTATCATC AGCATGACCT CCTTGACCCT GGGGAACATT
    CTACCCATTG CTGAAAATAA AAATGCTCAG GTTAATGGGC CCTTGATATC CATTATTATC
    CCAAACTCTT CCATCCATGA AATTTCCCTG ACTTTTTCTA AGATGAAATT GAATCTGAGT
    CAACCTCAAT GTGTGTTTTG GGATTTCAGT CATTTGCAGT GGACAGATAC CGGCTGCCAC
    CTAGAGAATG AAACTGCAAA CTTGGTGACA TGCCTTTGTA CTCACCTGAC CTCCTACTCC
    GTGCTGATGT CCTCTATTGT CCCTCCTGCC ATCGTCCCCA TTGTGAAATG GATCACCTTT
    GTGGGACTGG GGGTCTCCAT CGGAAGTCTC ATCTTATGCC TGACCATCCA AGGTCTGTTT
    TGGAAGCAGG TCAACAAAAA CCAAACCTCA CACAGCCGTC ACATCTGCAT AGTGAACATA
    GCTCTGTGCC TCCTGATTGC AGACGTCTGG TTTATTGTTG CTGCCACGGT GGACACAAAC
    TCTGGCGTCT GCACAGCTGC CGTGTTCTTT GCACACCTTT TCTACCTCTC CTTGTTCTTC
    TGGATGCTTC TGCTCGGGTT CCTGCTGGCA TATCGGGTGA TCTTCGTGTT CCATCACATG
    GCCACACCTT TGATGGTGGC TGTCGGGTTC TGCTTGGGCT ACGGGTGCCC TCTCATTATA
    TCCATCGTCA CCTTTCTGGT CACGCAGCCC AGCGATGGCT ACAAAAGGAA GGATGCATGT
    TGGCTTAACT GGTCCGATGG GAGCAAGCCT CTCTTGGCCT TTGCGGTTCC TGCCCTGACT
    ATTGTGGCTG TAAATTTGGT GGTGGTGCTA TTAGTTCTCA CGAAGCTCTG GAGGCCAGCT
    GTTGGAGAAA GGCTGGCCCA GGACAACAAG GCCACTGTCA AGCGCATAGG GAGGAGCCTC
    CTCGTCCTGA CCCCTCTTCT GGGGCTCACC TGGGGTTTCG GCATAGTAAC AATGGCGGAC
    GGCCAGAATC TGTCTTGGCA TGTTATTTTC GCATTTCTCA ATGCATTCCA GGGTCTTTTC
    ATCTTGTGTT TTGGAATTCT CTTGGATAGT AAGCTGCGAC AGCTGCTGCT CAACAAGTTG
    TCTCCTTTAA GCTGTTGGGA CCAGACATCA AAGCAGAAGT TATCAGATTC ATCTGTCACA
    CTCAGATTCG AAAAGCCTTT CAACCCATTC CAGCAGAAAG GCTGCTATGT ATTTTCATAT
    ACTAGCGAAT CCTCACATGA CATCATGCTA ACTCAGTTTT CATCAGCTGA ACAAAGCGGG
    GAATCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.