GCLC cDNA ORF clone, Bos taurus(cattle)

The following GCLC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GCLC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39035 NM_001083674.1
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa31873 XM_005223236.4
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa31874 XM_005223237.4
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa229467 XM_024983546.1
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa229468 XM_024983547.1
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
OBa229469 XM_024983548.1
Latest version!
Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00
View Old Accession Versions >>
OBa31873 XM_005223236.3 Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa31874 XM_005223237.3 Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39035
    Clone ID Related Accession (Same CDS sequence) NM_001083674.1
    Accession Version NM_001083674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY957499.1. On Apr 5, 2007 this sequence version replaced XM_865715.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAC TCCCCACCTG CCCAAGGATC ACCCGGGCCC AGCCCAGGTA
    ACGTTCCAAG CCTGCAGCAT ATCTGAGGCC AGATACCTTT ACGATCAGTT GGCTACCATC
    TGCCCAATTG TCATGGCTTT GAGTGCTGCG TCGCCTTTTT ACCGAGGCTA CGTGTCAGAC
    ATTGATTGTC GCTGGGGCGT GATTTCCGCG TCTGTAGATG ATAGAACTCG GGAGGAGAGA
    GGACTGGAGC CACTGAAGAA CAACCACTAT AGGATCAGTA AGTCCCGATA CGATTCGATA
    GACAGTTACT TGTCTGAGTG TGGTGAGAAA TACAATGACA TTGACCTGAC AAAAGACAAA
    GAAATCTACG AACAGCTGTT GCAGGAAGGC CTCGACCACC TGCTGGCCCA GCACGTGGCG
    CATCTCTTCA TCAGAGACCC GCTGACTCTG TTTGAAGAGA AAATCCACCT GGATGATGCC
    AACGAGTCCG ACCACTTTGA GAATATTCAG TCCACAAATT GGCAGACAAT GCGATTCAAG
    CCTCCTCCTC CAAACTCTGA CATTGGGTGG AGAGTGGAAT TCCGGCCCAT GGAGGTTCAG
    CTGACAGACT TCGAGAACTC CGCGTATGTG GTGTTCGTGG TGCTGCTCAC CAGGGTGATC
    CTGTCCTACA AACTGGACTT TCTCATCCCG CTGTCCAAGG TTGATGAGAA CATGAAGGTC
    GCCCAGAAGC GAGATGCCGT CCTGCAGGAA CTGTTTTATT TCAGGAAAGA CATTTGCAAA
    GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG
    GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC
    GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG
    TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA
    GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA
    ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG
    TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA
    ACCGACTCTT CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001077143.1
    CDS1..1818
    Translation

    Target ORF information:

    RefSeq Version NM_001083674.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001083674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAC TCCCCACCTG CCCAAGGATC ACCCGGGCCC AGCCCAGGTA
    ACGTTCCAAG CCTGCAGCAT ATCTGAGGCC AGATACCTTT ACGATCAGTT GGCTACCATC
    TGCCCAATTG TCATGGCTTT GAGTGCTGCG TCGCCTTTTT ACCGAGGCTA CGTGTCAGAC
    ATTGATTGTC GCTGGGGCGT GATTTCCGCG TCTGTAGATG ATAGAACTCG GGAGGAGAGA
    GGACTGGAGC CACTGAAGAA CAACCACTAT AGGATCAGTA AGTCCCGATA CGATTCGATA
    GACAGTTACT TGTCTGAGTG TGGTGAGAAA TACAATGACA TTGACCTGAC AAAAGACAAA
    GAAATCTACG AACAGCTGTT GCAGGAAGGC CTCGACCACC TGCTGGCCCA GCACGTGGCG
    CATCTCTTCA TCAGAGACCC GCTGACTCTG TTTGAAGAGA AAATCCACCT GGATGATGCC
    AACGAGTCCG ACCACTTTGA GAATATTCAG TCCACAAATT GGCAGACAAT GCGATTCAAG
    CCTCCTCCTC CAAACTCTGA CATTGGGTGG AGAGTGGAAT TCCGGCCCAT GGAGGTTCAG
    CTGACAGACT TCGAGAACTC CGCGTATGTG GTGTTCGTGG TGCTGCTCAC CAGGGTGATC
    CTGTCCTACA AACTGGACTT TCTCATCCCG CTGTCCAAGG TTGATGAGAA CATGAAGGTC
    GCCCAGAAGC GAGATGCCGT CCTGCAGGAA CTGTTTTATT TCAGGAAAGA CATTTGCAAA
    GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG
    GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC
    GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG
    TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA
    GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA
    ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG
    TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA
    ACCGACTCTT CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31873
    Clone ID Related Accession (Same CDS sequence) XM_005223236.4 , XM_005223236.3
    Accession Version XM_005223236.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223236.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC
    GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC
    ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC
    TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG
    AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG
    TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT
    AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC
    GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223293.1
    CDS29..1945
    Translation

    Target ORF information:

    RefSeq Version XM_005223236.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223236.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC
    GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC
    ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC
    TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG
    AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG
    TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT
    AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC
    GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31873
    Clone ID Related Accession (Same CDS sequence) XM_005223236.4 , XM_005223236.3
    Accession Version XM_005223236.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1917bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223236.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC
    GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC
    ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC
    TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG
    AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG
    TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT
    AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC
    GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223293.1
    CDS448..2364
    Translation

    Target ORF information:

    RefSeq Version XM_005223236.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223236.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGCAG GTGGCAACGC CGTGGTGGAC
    GGCTGCGGCA AGGCCGCGGG CAGGGCGGAG CCAGCGGCGG AGGAGTACGC GCTCATGACC
    ATCGACACCA TCATCAACGG CAAGGAAGGC GTGTTCCCCG GGCTGATCCC CGTCCTGAAC
    TCCTACCTGG AGAGCATGGA GGTGGACGTG GACACCAGGT GCAGCATTCT GAACTACCTG
    AAGCTCATCA AGAAGAGGGC GTCCGGAGAA TTAATGACAG TTGCCAAATG GATGAGGGAG
    TTTATCGCAA AGCATCCCGA CTACAAGCAG GACAGTGTAA TAACTGACCA GATGAACTAT
    AGCCTTCTTC TAAAGTGTAA TCAAATCGCA AACGAATCGT GTGAGTGCCC AGAGTTACTC
    GGACCAGCGT TTAGGAAAGT CAGGTACAGT GGAAGTAAAA CCGACTCTTC CACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31874
    Clone ID Related Accession (Same CDS sequence) XM_005223237.4 , XM_005223237.3
    Accession Version XM_005223237.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005223237.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC
    TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC
    GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC
    TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG
    CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT
    ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC
    CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA
    CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223294.1
    CDS1..1914
    Translation

    Target ORF information:

    RefSeq Version XM_005223237.4
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223237.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC
    TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC
    GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC
    TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG
    CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT
    ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC
    CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA
    CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa31874
    Clone ID Related Accession (Same CDS sequence) XM_005223237.4 , XM_005223237.3
    Accession Version XM_005223237.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1914bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104548.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005223237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC
    TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC
    GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC
    TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG
    CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT
    ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC
    CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA
    CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005223294.1
    CDS448..2361
    Translation

    Target ORF information:

    RefSeq Version XM_005223237.3
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase, catalytic subunit (GCLC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005223237.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGATTA GGCTGCCCTG GGTTCACGCT GCCCGAGTTC
    AAACCCAACC CGGTGGAAGG AGGAGCTTCC AAGTCCCTTT TCTTTCCTGA TGAAGCCATC
    AACAAGCACC CGCGCTTCAG CACGCTCACC AGGAACATCC GGCACCGGAG GGGCGAGAAA
    GTGGTCATCA ACGTGCCCAT CTTTAAGGAC AAGAACACCC CAGCTCCATT TATAGAGACT
    TTCCCTGAGG ACGAGGAGGC CACGAAGGCC TCTAAGCCCG ACCACATTTA CATGGACGCC
    ATGGGCTTCG GGATGGGCAA CTGCTGCCTA CAGGTAACGT TCCAAGCCTG CAGCATATCT
    GAGGCCAGAT ACCTTTACGA TCAGTTGGCT ACCATCTGCC CAATTGTCAT GGCTTTGAGT
    GCTGCGTCGC CTTTTTACCG AGGCTACGTG TCAGACATTG ATTGTCGCTG GGGCGTGATT
    TCCGCGTCTG TAGATGATAG AACTCGGGAG GAGAGAGGAC TGGAGCCACT GAAGAACAAC
    CACTATAGGA TCAGTAAGTC CCGATACGAT TCGATAGACA GTTACTTGTC TGAGTGTGGT
    GAGAAATACA ATGACATTGA CCTGACAAAA GACAAAGAAA TCTACGAACA GCTGTTGCAG
    GAAGGCCTCG ACCACCTGCT GGCCCAGCAC GTGGCGCATC TCTTCATCAG AGACCCGCTG
    ACTCTGTTTG AAGAGAAAAT CCACCTGGAT GATGCCAACG AGTCCGACCA CTTTGAGAAT
    ATTCAGTCCA CAAATTGGCA GACAATGCGA TTCAAGCCTC CTCCTCCAAA CTCTGACATT
    GGGTGGAGAG TGGAATTCCG GCCCATGGAG GTTCAGCTGA CAGACTTCGA GAACTCCGCG
    TATGTGGTGT TCGTGGTGCT GCTCACCAGG GTGATCCTGT CCTACAAACT GGACTTTCTC
    ATCCCGCTGT CCAAGGTTGA TGAGAACATG AAGGTCGCCC AGAAGCGAGA TGCCGTCCTG
    CAGGAACTGT TTTATTTCAG GAAAGACATT TGCAAAGGTG GCAACGCCGT GGTGGACGGC
    TGCGGCAAGG CCGCGGGCAG GGCGGAGCCA GCGGCGGAGG AGTACGCGCT CATGACCATC
    GACACCATCA TCAACGGCAA GGAAGGCGTG TTCCCCGGGC TGATCCCCGT CCTGAACTCC
    TACCTGGAGA GCATGGAGGT GGACGTGGAC ACCAGGTGCA GCATTCTGAA CTACCTGAAG
    CTCATCAAGA AGAGGGCGTC CGGAGAATTA ATGACAGTTG CCAAATGGAT GAGGGAGTTT
    ATCGCAAAGC ATCCCGACTA CAAGCAGGAC AGTGTAATAA CTGACCAGAT GAACTATAGC
    CTTCTTCTAA AGTGTAATCA AATCGCAAAC GAATCGTGTG AGTGCCCAGA GTTACTCGGA
    CCAGCGTTTA GGAAAGTCAG GTACAGTGGA AGTAAAACCG ACTCTTCCAC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229467
    Clone ID Related Accession (Same CDS sequence) XM_024983546.1
    Accession Version XM_024983546.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1935bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT
    TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG
    GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT
    TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC
    TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC
    TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG
    TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT
    CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA
    GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC
    TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG
    AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGCAGGT
    GGCAACGCCG TGGTGGACGG CTGCGGCAAG GCCGCGGGCA GGGCGGAGCC AGCGGCGGAG
    GAGTACGCGC TCATGACCAT CGACACCATC ATCAACGGCA AGGAAGGCGT GTTCCCCGGG
    CTGATCCCCG TCCTGAACTC CTACCTGGAG AGCATGGAGG TGGACGTGGA CACCAGGTGC
    AGCATTCTGA ACTACCTGAA GCTCATCAAG AAGAGGGCGT CCGGAGAATT AATGACAGTT
    GCCAAATGGA TGAGGGAGTT TATCGCAAAG CATCCCGACT ACAAGCAGGA CAGTGTAATA
    ACTGACCAGA TGAACTATAG CCTTCTTCTA AAGTGTAATC AAATCGCAAA CGAATCGTGT
    GAGTGCCCAG AGTTACTCGG ACCAGCGTTT AGGAAAGTCA GGTACAGTGG AAGTAAAACC
    GACTCTTCCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839314.1
    CDS473..2407
    Translation

    Target ORF information:

    RefSeq Version XM_024983546.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983546.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT
    TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG
    GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT
    TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC
    TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC
    TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG
    TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT
    CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA
    GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC
    TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG
    AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGCAGGT
    GGCAACGCCG TGGTGGACGG CTGCGGCAAG GCCGCGGGCA GGGCGGAGCC AGCGGCGGAG
    GAGTACGCGC TCATGACCAT CGACACCATC ATCAACGGCA AGGAAGGCGT GTTCCCCGGG
    CTGATCCCCG TCCTGAACTC CTACCTGGAG AGCATGGAGG TGGACGTGGA CACCAGGTGC
    AGCATTCTGA ACTACCTGAA GCTCATCAAG AAGAGGGCGT CCGGAGAATT AATGACAGTT
    GCCAAATGGA TGAGGGAGTT TATCGCAAAG CATCCCGACT ACAAGCAGGA CAGTGTAATA
    ACTGACCAGA TGAACTATAG CCTTCTTCTA AAGTGTAATC AAATCGCAAA CGAATCGTGT
    GAGTGCCCAG AGTTACTCGG ACCAGCGTTT AGGAAAGTCA GGTACAGTGG AAGTAAAACC
    GACTCTTCCA CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229468
    Clone ID Related Accession (Same CDS sequence) XM_024983547.1
    Accession Version XM_024983547.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1932bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT
    TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG
    GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT
    TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC
    TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC
    TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG
    TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT
    CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA
    GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC
    TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG
    AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGGTGGC
    AACGCCGTGG TGGACGGCTG CGGCAAGGCC GCGGGCAGGG CGGAGCCAGC GGCGGAGGAG
    TACGCGCTCA TGACCATCGA CACCATCATC AACGGCAAGG AAGGCGTGTT CCCCGGGCTG
    ATCCCCGTCC TGAACTCCTA CCTGGAGAGC ATGGAGGTGG ACGTGGACAC CAGGTGCAGC
    ATTCTGAACT ACCTGAAGCT CATCAAGAAG AGGGCGTCCG GAGAATTAAT GACAGTTGCC
    AAATGGATGA GGGAGTTTAT CGCAAAGCAT CCCGACTACA AGCAGGACAG TGTAATAACT
    GACCAGATGA ACTATAGCCT TCTTCTAAAG TGTAATCAAA TCGCAAACGA ATCGTGTGAG
    TGCCCAGAGT TACTCGGACC AGCGTTTAGG AAAGTCAGGT ACAGTGGAAG TAAAACCGAC
    TCTTCCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839315.1
    CDS473..2404
    Translation

    Target ORF information:

    RefSeq Version XM_024983547.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983547.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCATGG CTTTGAGTGC TGCGTCGCCT TTTTACCGAG GCTACGTGTC AGACATTGAT
    TGTCGCTGGG GCGTGATTTC CGCGTCTGTA GATGATAGAA CTCGGGAGGA GAGAGGACTG
    GAGCCACTGA AGAACAACCA CTATAGGATC AGTAAGTCCC GATACGATTC GATAGACAGT
    TACTTGTCTG AGTGTGGTGA GAAATACAAT GACATTGACC TGACAAAAGA CAAAGAAATC
    TACGAACAGC TGTTGCAGGA AGGCCTCGAC CACCTGCTGG CCCAGCACGT GGCGCATCTC
    TTCATCAGAG ACCCGCTGAC TCTGTTTGAA GAGAAAATCC ACCTGGATGA TGCCAACGAG
    TCCGACCACT TTGAGAATAT TCAGTCCACA AATTGGCAGA CAATGCGATT CAAGCCTCCT
    CCTCCAAACT CTGACATTGG GTGGAGAGTG GAATTCCGGC CCATGGAGGT TCAGCTGACA
    GACTTCGAGA ACTCCGCGTA TGTGGTGTTC GTGGTGCTGC TCACCAGGGT GATCCTGTCC
    TACAAACTGG ACTTTCTCAT CCCGCTGTCC AAGGTTGATG AGAACATGAA GGTCGCCCAG
    AAGCGAGATG CCGTCCTGCA GGAACTGTTT TATTTCAGGA AAGACATTTG CAAAGGTGGC
    AACGCCGTGG TGGACGGCTG CGGCAAGGCC GCGGGCAGGG CGGAGCCAGC GGCGGAGGAG
    TACGCGCTCA TGACCATCGA CACCATCATC AACGGCAAGG AAGGCGTGTT CCCCGGGCTG
    ATCCCCGTCC TGAACTCCTA CCTGGAGAGC ATGGAGGTGG ACGTGGACAC CAGGTGCAGC
    ATTCTGAACT ACCTGAAGCT CATCAAGAAG AGGGCGTCCG GAGAATTAAT GACAGTTGCC
    AAATGGATGA GGGAGTTTAT CGCAAAGCAT CCCGACTACA AGCAGGACAG TGTAATAACT
    GACCAGATGA ACTATAGCCT TCTTCTAAAG TGTAATCAAA TCGCAAACGA ATCGTGTGAG
    TGCCCAGAGT TACTCGGACC AGCGTTTAGG AAAGTCAGGT ACAGTGGAAG TAAAACCGAC
    TCTTCCACTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa229469
    Clone ID Related Accession (Same CDS sequence) XM_024983548.1
    Accession Version XM_024983548.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1818bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product glutamate--cysteine ligase catalytic subunit isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCCCAC TGAAGAACAA CCACTATAGG ATCAGTAAGT CCCGATACGA TTCGATAGAC
    AGTTACTTGT CTGAGTGTGG TGAGAAATAC AATGACATTG ACCTGACAAA AGACAAAGAA
    ATCTACGAAC AGCTGTTGCA GGAAGGCCTC GACCACCTGC TGGCCCAGCA CGTGGCGCAT
    CTCTTCATCA GAGACCCGCT GACTCTGTTT GAAGAGAAAA TCCACCTGGA TGATGCCAAC
    GAGTCCGACC ACTTTGAGAA TATTCAGTCC ACAAATTGGC AGACAATGCG ATTCAAGCCT
    CCTCCTCCAA ACTCTGACAT TGGGTGGAGA GTGGAATTCC GGCCCATGGA GGTTCAGCTG
    ACAGACTTCG AGAACTCCGC GTATGTGGTG TTCGTGGTGC TGCTCACCAG GGTGATCCTG
    TCCTACAAAC TGGACTTTCT CATCCCGCTG TCCAAGGTTG ATGAGAACAT GAAGGTCGCC
    CAGAAGCGAG ATGCCGTCCT GCAGGAACTG TTTTATTTCA GGAAAGACAT TTGCAAAGCA
    GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG
    GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC
    GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG
    TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA
    GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA
    ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG
    TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA
    ACCGACTCTT CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024839316.1
    CDS474..2291
    Translation

    Target ORF information:

    RefSeq Version XM_024983548.1
    Organism Bos taurus(cattle)
    Definition Bos taurus glutamate-cysteine ligase catalytic subunit (GCLC), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024983548.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGGGCTGC TGTCCCAGGG CTCGCCGCTG AGCTGGGAGG AGACCCAGCG CCACGCCGAC 
    CACGTGCGGC GGCACGGGAT CCTCCAGTTC CTGCACATCT ACCACGCCGT CAAGGACCGG
    CACAAGGACG TGCTCAAGTG GGGCGATGAG GTGGAGTACA TGTTGGTGTC TTTTGATCAT
    GAAAAGAAAA AAGCCCAACT GGTCCTGTCT GGAGAGGAAC TTCTTGAAAC TCTGCAAGAA
    AAGGGGGAAA GGACGAACCC AAACCATCCA ACCCTTTGGA GACCAGAATA TGGGAGCTAC
    ATGATCGAGG GGACACCCGG GCAGCCGTAC GGTGGGACCA TGTCTGAATT CAACACAGTT
    GAGGACAACA TGAGGAAACG GCGCAAAGAA GCCACATCTC TCCTGGGGGA AAACCAGGCC
    CTCTGCACGA TCACTTCCTT TCCCAGTTCT TCAATTTGTT GTAGATTAGG CTGCCCTGGG
    TTCACGCTGC CCGAGTTCAA ACCCAACCCG GTGGAAGGAG GAGCTTCCAA GTCCCTTTTC
    TTTCCTGATG AAGCCATCAA CAAGCACCCG CGCTTCAGCA CGCTCACCAG GAACATCCGG
    CACCGGAGGG GCGAGAAAGT GGTCATCAAC GTGCCCATCT TTAAGGACAA GAACACCCCA
    GCTCCATTTA TAGAGACTTT CCCTGAGGAC GAGGAGGCCA CGAAGGCCTC TAAGCCCGAC
    CACATTTACA TGGACGCCAT GGGCTTCGGG ATGGGCAACT GCTGCCTACA GGTAACGTTC
    CAAGCCTGCA GCATATCTGA GGCCAGATAC CTTTACGATC AGTTGGCTAC CATCTGCCCA
    ATTGTCCCAC TGAAGAACAA CCACTATAGG ATCAGTAAGT CCCGATACGA TTCGATAGAC
    AGTTACTTGT CTGAGTGTGG TGAGAAATAC AATGACATTG ACCTGACAAA AGACAAAGAA
    ATCTACGAAC AGCTGTTGCA GGAAGGCCTC GACCACCTGC TGGCCCAGCA CGTGGCGCAT
    CTCTTCATCA GAGACCCGCT GACTCTGTTT GAAGAGAAAA TCCACCTGGA TGATGCCAAC
    GAGTCCGACC ACTTTGAGAA TATTCAGTCC ACAAATTGGC AGACAATGCG ATTCAAGCCT
    CCTCCTCCAA ACTCTGACAT TGGGTGGAGA GTGGAATTCC GGCCCATGGA GGTTCAGCTG
    ACAGACTTCG AGAACTCCGC GTATGTGGTG TTCGTGGTGC TGCTCACCAG GGTGATCCTG
    TCCTACAAAC TGGACTTTCT CATCCCGCTG TCCAAGGTTG ATGAGAACAT GAAGGTCGCC
    CAGAAGCGAG ATGCCGTCCT GCAGGAACTG TTTTATTTCA GGAAAGACAT TTGCAAAGCA
    GGTGGCAACG CCGTGGTGGA CGGCTGCGGC AAGGCCGCGG GCAGGGCGGA GCCAGCGGCG
    GAGGAGTACG CGCTCATGAC CATCGACACC ATCATCAACG GCAAGGAAGG CGTGTTCCCC
    GGGCTGATCC CCGTCCTGAA CTCCTACCTG GAGAGCATGG AGGTGGACGT GGACACCAGG
    TGCAGCATTC TGAACTACCT GAAGCTCATC AAGAAGAGGG CGTCCGGAGA ATTAATGACA
    GTTGCCAAAT GGATGAGGGA GTTTATCGCA AAGCATCCCG ACTACAAGCA GGACAGTGTA
    ATAACTGACC AGATGAACTA TAGCCTTCTT CTAAAGTGTA ATCAAATCGC AAACGAATCG
    TGTGAGTGCC CAGAGTTACT CGGACCAGCG TTTAGGAAAG TCAGGTACAG TGGAAGTAAA
    ACCGACTCTT CCACTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.