OCLN cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	OCLN
Entrez Gene ID	512405
Full Name	occludin
General protein information	Preferred Names occludin Names occludin tight-junction protein
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221439.4	XP_005221496.1	occludin isoform X1
NM_001082433.2	NP_001075902.1	occludin
XM_005221439.3	XP_005221496.1	occludin isoform X1
XM_005221440.3	XP_005221497.1	occludin isoform X1
XM_024981258.1	XP_024837026.1	occludin isoform X1
XM_005221440.4	XP_005221497.1	occludin isoform X1

Pathways from BioSystems

Homologies

	OCLN	XP_001094789.1
Xenopus tropicalis (tropical clawed frog)	ocln	NP_001015948.1
Rattus norvegicus (Norway rat)	Ocln	NP_112619.2
Danio rerio (zebrafish)	oclna	NP_997997.2
Mus musculus (house mouse)	Ocln	NP_032782.1
Homo sapiens (human)	OCLN	NP_001192183.1
Pan troglodytes (chimpanzee)	OCLN	XP_001158455.1
Canis lupus familiaris (dog)	OCLN	NP_001003195.1
Bos taurus (cattle)	OCLN	NP_001075902.1

Gene Ontology
Bibliography

Related articles in PubMed

Effects of asymmetric dimethylarginine on bovine retinal capillary endothelial cell proliferation, reactive oxygen species production, permeability, intercellular adhesion molecule-1, and occludin expression.
Chen YH, Xu X, Sheng MJ, Zheng Z, Gu Q
Molecular vision17332-40(2011 Feb)

Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability.
Murakami T, Felinski EA, Antonetti DA
The Journal of biological chemistry284(31)21036-46(2009 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Cyclic strain-mediated regulation of vascular endothelial occludin and ZO-1: influence on intercellular tight junction assembly and function.
Collins NT, Cummins PM, Colgan OC, Ferguson G, Birney YA, Murphy RP, Meade G, Cahill PA
Arteriosclerosis, thrombosis, and vascular biology26(1)62-8(2006 Jan)

Hypoxia/aglycemia alters expression of occludin and actin in brain endothelial cells.
Brown RC, Davis TP
Biochemical and biophysical research communications327(4)1114-23(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ADMA has potent adverse effects on cell proliferation, intracellular ROS generation, cell permeability, levels of ICAM-1, and the tight-junction protein occludin
Title: Effects of asymmetric dimethylarginine on bovine retinal capillary endothelial cell proliferation, reactive oxygen species production, permeability, intercellular adhesion molecule-1, and occludin expression.

These data demonstrate a novel mechanism of VEGF-induced occludin phosphorylation and ubiquitination that contributes to TJ trafficking and subsequent vascular permeability.
Title: Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability.

cyclic strain modulates both the expression and phosphorylation state of occludin and ZO-1 in vascular endothelial cells, with putative consequences for endothelial tight junction assembly and barrier integrity
Title: Cyclic strain-mediated regulation of vascular endothelial occludin and ZO-1: influence on intercellular tight junction assembly and function.

the presence of occludin at the membrane is dependent in part on calcium-sensitive signaling cascades
Title: Hypoxia/aglycemia alters expression of occludin and actin in brain endothelial cells.

The following OCLN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OCLN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36443	NM_001082433.2 Latest version!	Bos taurus occludin (OCLN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa30567	XM_005221440.4 Latest version!	Bos taurus occludin (OCLN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa30567	XM_024981258.1 Latest version!	Bos taurus occludin (OCLN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa30567	XM_005221439.4 Latest version!	Bos taurus occludin (OCLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
View Old Accession Versions >>
OBa30567	XM_005221439.3	Bos taurus occludin (OCLN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
OBa30567	XM_005221440.3	Bos taurus occludin (OCLN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$342.30 $489.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OCLN ( NM_001082433.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075902.1 Bos taurus occludin (OCLN), mRNA.

Price & Availability↑

CloneID	OBa36443
Clone ID Related Accession (Same CDS sequence)	NM_001082433.2
Accession Version	NM_001082433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	occludin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133617.1. On Aug 30, 2012 this sequence version replaced NM_001082433.1. ##Evidence-Data-START## Transcript exon combination :: BC133617.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTACG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075902.1
CDS	235..1803
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	NM_001082433.2
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082433.2

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTACG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCLN ( XM_005221439.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221496.1 Bos taurus occludin (OCLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30567
Clone ID Related Accession (Same CDS sequence)	XM_005221439.4 , XM_005221439.3 , XM_005221440.3 , XM_024981258.1 , XM_005221440.4
Accession Version	XM_005221439.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	occludin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221439.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221496.1
CDS	192..1760
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	XM_005221439.4
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221439.4

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCLN ( XM_005221439.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221496.1 Bos taurus occludin (OCLN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30567
Clone ID Related Accession (Same CDS sequence)	XM_005221439.4 , XM_005221439.3 , XM_005221440.3 , XM_024981258.1 , XM_005221440.4
Accession Version	XM_005221439.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	occludin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221496.1
CDS	166..1734
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	XM_005221439.3
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221439.3

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCLN ( XM_005221440.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221497.1 Bos taurus occludin (OCLN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30567
Clone ID Related Accession (Same CDS sequence)	XM_005221439.4 , XM_005221439.3 , XM_005221440.3 , XM_024981258.1 , XM_005221440.4
Accession Version	XM_005221440.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	occludin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104506.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221497.1
CDS	147..1715
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	XM_005221440.3
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221440.3

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCLN ( XM_024981258.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024837026.1 Bos taurus occludin (OCLN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa30567
Clone ID Related Accession (Same CDS sequence)	XM_005221439.4 , XM_005221439.3 , XM_005221440.3 , XM_024981258.1 , XM_005221440.4
Accession Version	XM_024981258.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	occludin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024837026.1
CDS	90..1658
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	XM_024981258.1
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981258.1

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OCLN ( XM_005221440.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221497.1 Bos taurus occludin (OCLN), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30567
Clone ID Related Accession (Same CDS sequence)	XM_005221439.4 , XM_005221439.3 , XM_005221440.3 , XM_024981258.1 , XM_005221440.4
Accession Version	XM_005221440.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1569bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	occludin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221497.1
CDS	149..1717
Translation	MSSRPFESPPPYRPDEFKPNHYTPSNDIYSGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFACVASTLAWDRGYGTLMGAGVNYPYAGSAFGSYGSGYGYGYGYGYGYGTGYTDPRAAKGFLLAMAAFCFIAALVIFVTSVVRSGISRTRRYYLTVIIVTAVLGIMMFIATIVYIMGVNPTAQASGSLYSSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMVIVAFALMIFFAVKTRRKMNCYDKSNILWDKERIYDEQPPNVEEWVKNVSAGTQDMPPPLSDYVERVDSPVAYSSNGKVNEKRLYPESSYKSTPVPEVAQELPLTSPVEDFRQPHYSSSGNLETLSKRAPAKGRAGKSRRAEQDHYETDYTTGGESCDELEDDWIREYPPITSDQQRQLYKRSFDTGLQEYKSLQAELDEVNKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKRNYCKQLKSKLSHIKKMVGDYDRRKT

Target ORF information:

RefSeq Version	XM_005221440.4
Organism	Bos taurus(cattle)
Definition	Bos taurus occludin (OCLN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221440.4

ORF Insert Sequence:

ATGTCATCCA GGCCTTTTGA AAGTCCACCT CCTTATAGAC CTGATGAATT CAAACCTAAT 
CACTACACAC CAAGCAATGA CATATATAGT GGAGAGATGC ACGTTCGACC AATGCTCTCT 
CAGCCAGCAT ATTCCTTCTA CCCGGAAGAT GAAATTCTCC ACTTCTACAA ATGGACCTCT 
CCTCCCGGAG TAATTCGGAT CCTGTCTATG CTTGTTATCG TGATGTGCAT CGCCATTTTC 
GCCTGTGTTG CCTCCACTCT TGCCTGGGAC AGAGGCTATG GAACCTTAAT GGGAGCTGGT 
GTTAACTACC CTTATGCAGG AAGTGCCTTT GGTAGCTACG GAAGTGGCTA TGGCTACGGC 
TATGGCTATG GTTATGGCTA TGGAACAGGC TATACAGATC CCAGAGCGGC AAAGGGATTC 
CTGCTGGCCA TGGCTGCCTT TTGTTTCATT GCTGCATTGG TGATATTTGT TACCAGCGTT 
GTAAGGTCAG GCATATCTAG AACAAGAAGG TATTATTTGA CTGTGATAAT AGTGACCGCC 
GTCCTGGGCA TTATGATGTT TATTGCCACA ATTGTCTATA TAATGGGAGT CAATCCAACT 
GCCCAGGCCT CTGGGTCTCT CTACAGTTCA CAGATATATG CCCTCTGCAA CCAGTTTTAT 
ACACCTGCAG CTACTGGACT CTACGTGGAT CAATATTTAT ATCACTACTG TGTGGTGGAT 
CCCCAGGAGG CCATTGCCAT TGTACTGGGG TTCATGGTTA TTGTGGCTTT TGCTCTAATG 
ATTTTCTTTG CTGTGAAAAC TCGAAGAAAG ATGAACTGCT ATGACAAGTC AAATATTCTG 
TGGGACAAGG AACGTATTTA TGATGAGCAG CCTCCCAATG TTGAAGAGTG GGTTAAAAAC 
GTTTCTGCAG GTACACAAGA CATGCCTCCT CCCCTCTCAG ACTATGTGGA GAGAGTGGAC 
AGCCCAGTGG CCTACTCCTC CAATGGTAAA GTGAACGAGA AGCGACTGTA TCCAGAGTCT 
TCCTATAAAT CAACCCCGGT GCCGGAAGTG GCTCAGGAGC TGCCATTGAC TTCACCTGTG 
GAGGACTTCC GGCAGCCTCA TTACAGCAGC AGTGGTAACT TGGAGACGCT TTCAAAAAGG 
GCTCCCGCGA AGGGCAGGGC AGGGAAGTCA AGGCGGGCAG AGCAAGACCA CTATGAAACA 
GACTACACGA CCGGAGGCGA GTCCTGCGAT GAGCTGGAGG ACGACTGGAT CAGGGAATAT 
CCACCTATCA CTTCAGATCA ACAAAGACAA CTCTACAAGA GAAGTTTTGA CACTGGCCTA 
CAGGAATATA AGAGCCTACA AGCAGAACTT GATGAGGTCA ATAAAGAACT CTCTCGTCTG 
GATAAAGAAC TGGATGACTA TAGAGAAGAA AGTGAAGAGT ACATGGCTGC TGCCGACGAG 
TACAATAGAC TGAAACAAGT TAAGGGATCA GCAGATTACA AAAGTAAGAG GAATTACTGT 
AAACAGTTGA AGAGCAAATT GTCACACATT AAGAAGATGG TTGGAGATTA CGACCGACGG 
AAAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effects of asymmetric dimethylarginine on bovine retinal capillary endothelial cell proliferation, reactive oxygen species production, permeability, intercellular adhesion molecule-1, and occludin expression.
Molecular vision17332-40(2011 Feb)
Chen YH,Xu X,Sheng MJ,Zheng Z,Gu Q

Occludin phosphorylation and ubiquitination regulate tight junction trafficking and vascular endothelial growth factor-induced permeability.
The Journal of biological chemistry284(31)21036-46(2009 Jul)
Murakami T,Felinski EA,Antonetti DA

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Cyclic strain-mediated regulation of vascular endothelial occludin and ZO-1: influence on intercellular tight junction assembly and function.
Arteriosclerosis, thrombosis, and vascular biology26(1)62-8(2006 Jan)
Collins NT,Cummins PM,Colgan OC,Ferguson G,Birney YA,Murphy RP,Meade G,Cahill PA

Hypoxia/aglycemia alters expression of occludin and actin in brain endothelial cells.
Biochemical and biophysical research communications327(4)1114-23(2005 Feb)
Brown RC,Davis TP

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OCLN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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