ARAP1 cDNA ORF clone, Bos taurus(cattle)

The following ARAP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARAP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa223289 XM_024975865.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223289 XM_024975866.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223289 XM_024975864.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223289 XM_015474774.2
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223289 XM_024975867.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223290 XM_024975862.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223290 XM_024975868.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223291 XM_024975863.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa223292 XM_024975869.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa223293 XM_024975870.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa92731 XM_005194645.3
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa92731 XM_015474774.1
Latest version!
Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa223289
    Clone ID Related Accession (Same CDS sequence) XM_015474774.2 , XM_024975865.1 , XM_024975864.1 , XM_024975867.1 , XM_024975866.1
    Accession Version XM_015474774.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330260.2
    CDS266..4633
    Translation

    Target ORF information:

    RefSeq Version XM_015474774.2
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474774.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223289
    Clone ID Related Accession (Same CDS sequence) XM_015474774.2 , XM_024975865.1 , XM_024975864.1 , XM_024975867.1 , XM_024975866.1
    Accession Version XM_024975865.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831633.1
    CDS188..4555
    Translation

    Target ORF information:

    RefSeq Version XM_024975865.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975865.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223289
    Clone ID Related Accession (Same CDS sequence) XM_015474774.2 , XM_024975865.1 , XM_024975864.1 , XM_024975867.1 , XM_024975866.1
    Accession Version XM_024975864.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831632.1
    CDS252..4619
    Translation

    Target ORF information:

    RefSeq Version XM_024975864.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975864.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223289
    Clone ID Related Accession (Same CDS sequence) XM_015474774.2 , XM_024975865.1 , XM_024975864.1 , XM_024975867.1 , XM_024975866.1
    Accession Version XM_024975867.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831635.1
    CDS66..4433
    Translation

    Target ORF information:

    RefSeq Version XM_024975867.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975867.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223289
    Clone ID Related Accession (Same CDS sequence) XM_015474774.2 , XM_024975865.1 , XM_024975864.1 , XM_024975867.1 , XM_024975866.1
    Accession Version XM_024975866.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831634.1
    CDS162..4529
    Translation

    Target ORF information:

    RefSeq Version XM_024975866.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975866.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223290
    Clone ID Related Accession (Same CDS sequence) XM_024975862.1 , XM_024975868.1
    Accession Version XM_024975862.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GTCACCGGCC TGAGAAGGAG TGGCCTGTCA GGAGTCTCAA AGTCTACCTG
    GGAGTGAAGA AGAAACTCCG GCCGCCCACC TGCTGGGGCT TCACGGTGAT ACACGAGACG
    GAGAAACACG AGAAGCAGCA GTGGTACCTG TGCTGTGAGA CACAGATGGA GCTCCGGGAG
    TGGCTCGCCA CCTTCCTCTT CGTGCAGCAC GACGGCCTGG TGTGGCCCTC GGAGCCCTCA
    CGCGTGTCCC GGGCAGCGCC TGAGGTCCGG CTGGGCAGCA TTTCATTGAT CCCCCTGCGA
    GGCAGTGAGA ATGAGATGCG CCGGAGTGTG GCCGCCTTTT CTGCGGACCC CCTTTCTCTT
    CTCCGAAACG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831630.1
    CDS266..4600
    Translation

    Target ORF information:

    RefSeq Version XM_024975862.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975862.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GTCACCGGCC TGAGAAGGAG TGGCCTGTCA GGAGTCTCAA AGTCTACCTG
    GGAGTGAAGA AGAAACTCCG GCCGCCCACC TGCTGGGGCT TCACGGTGAT ACACGAGACG
    GAGAAACACG AGAAGCAGCA GTGGTACCTG TGCTGTGAGA CACAGATGGA GCTCCGGGAG
    TGGCTCGCCA CCTTCCTCTT CGTGCAGCAC GACGGCCTGG TGTGGCCCTC GGAGCCCTCA
    CGCGTGTCCC GGGCAGCGCC TGAGGTCCGG CTGGGCAGCA TTTCATTGAT CCCCCTGCGA
    GGCAGTGAGA ATGAGATGCG CCGGAGTGTG GCCGCCTTTT CTGCGGACCC CCTTTCTCTT
    CTCCGAAACG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223290
    Clone ID Related Accession (Same CDS sequence) XM_024975862.1 , XM_024975868.1
    Accession Version XM_024975868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4335bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GTCACCGGCC TGAGAAGGAG TGGCCTGTCA GGAGTCTCAA AGTCTACCTG
    GGAGTGAAGA AGAAACTCCG GCCGCCCACC TGCTGGGGCT TCACGGTGAT ACACGAGACG
    GAGAAACACG AGAAGCAGCA GTGGTACCTG TGCTGTGAGA CACAGATGGA GCTCCGGGAG
    TGGCTCGCCA CCTTCCTCTT CGTGCAGCAC GACGGCCTGG TGTGGCCCTC GGAGCCCTCA
    CGCGTGTCCC GGGCAGCGCC TGAGGTCCGG CTGGGCAGCA TTTCATTGAT CCCCCTGCGA
    GGCAGTGAGA ATGAGATGCG CCGGAGTGTG GCCGCCTTTT CTGCGGACCC CCTTTCTCTT
    CTCCGAAACG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831636.1
    CDS178..4512
    Translation

    Target ORF information:

    RefSeq Version XM_024975868.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GTCACCGGCC TGAGAAGGAG TGGCCTGTCA GGAGTCTCAA AGTCTACCTG
    GGAGTGAAGA AGAAACTCCG GCCGCCCACC TGCTGGGGCT TCACGGTGAT ACACGAGACG
    GAGAAACACG AGAAGCAGCA GTGGTACCTG TGCTGTGAGA CACAGATGGA GCTCCGGGAG
    TGGCTCGCCA CCTTCCTCTT CGTGCAGCAC GACGGCCTGG TGTGGCCCTC GGAGCCCTCA
    CGCGTGTCCC GGGCAGCGCC TGAGGTCCGG CTGGGCAGCA TTTCATTGAT CCCCCTGCGA
    GGCAGTGAGA ATGAGATGCG CCGGAGTGTG GCCGCCTTTT CTGCGGACCC CCTTTCTCTT
    CTCCGAAACG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223291
    Clone ID Related Accession (Same CDS sequence) XM_024975863.1
    Accession Version XM_024975863.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4218bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831631.1
    CDS266..4483
    Translation

    Target ORF information:

    RefSeq Version XM_024975863.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975863.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCAGGC CCTGCAGCAG
    GCGGTGGCTG AGCAGCGTGC CCGGGCCCGC GTGTCTAGTG CTTACCCATT GGGTGTTCAA
    GGCTCAGAGC CCCATGACCA TGCTGGCAGC CTGGAGCTAC GTGGCTTCAA GAATAAACTC
    TATGTGGCCG TGGTCGGGGA CAAAGTTGAG CTTTACAAGA ATCTGGAGGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223292
    Clone ID Related Accession (Same CDS sequence) XM_024975869.1
    Accession Version XM_024975869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACCAAGA AGGAGGAGCC CCCTGCGAGT CAAGTCCCAC GGGCCGTGCG TGTGGCCAGT 
    CTGCTGAGCG AAGGGGAGGA CCTGTCTGGA GATGACCAAG GGGATGAAGA TGAGGATGAC
    TATGCCTACG AGCGTGTCCC CAATGGTGAC TGGCACACCA GCAGCCTGAG CTCATCTTTG
    CCCAGCAGCC TCCTGACCCC TGAGCTTCCC CCGCACCCCA TGGACGGGCT GCCCGTGGGC
    CCCACACCCA CCCCACTGGT CATCAAGGCT GGCTGGCTGG ACAAGAACCC CCCGCAGGGA
    TCTTACATCT ATCAGAAGCG ATGGGTGAGA CTGGATGCCA ATCACCTGCG ATACTTTGAT
    AGTAACAAGG ATGCCTACTC AAAGCGCTTT GTCCCCGTGG CCTGCATCTC CAGAGTGGCT
    GCCATTGGGG ACCAAAAGTT CGAAGTGATC ACGAACAACC GGACCTTTGC CTTCCGGGCA
    GAGAGTGATG CGGAGCGGAA GGAGTGGATG CAGGCCCTGC AGCAGGCGGT GGCTGAGCAG
    CGTGCCCGGG CCCGCGTGTC TAGTGCTTAC CCATTGGGTG TTCAAGGCTC AGAGCCCCAT
    GACCATGCTG GCAGCCTGGA GCTACGTGGC TTCAAGAATA AACTCTATGT GGCCGTGGTC
    GGGGACAAAG TTGAGCTTTA CAAGAATCTG GAGGAGTACC GCCTGGGCAT CGGCATCACC
    TTCATTGACA TGAGTGTGGG CAACGTGAAG GAAGCGGCCG ACCGGCGCAG CTTCGACCTC
    ACCACCCCCT ACCGCATCTT CAGCTTCTCA GCCGAATCGG AGCTGGAGAA GGAGCAGTGG
    CTGGAGGCCA TGCAGGGAGC CATCGCCGAG GCCCTGTCCA CCTCGGAGGT GGCCGAGCGC
    ATCTGGGCCA TGGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTGCCCA GCCTGACTGG
    GCCTCCATCA ATCTCTGTGT TGTCATCTGT AAGCGCTGTG CAGGAGAGCA CCGTGGCCTG
    GGCGCTGGTA TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACGGAAACA
    CTCATCGAGC TCTTCTTACA GCTGGGCAAT GCTGCCGGGA ACCACTTCTG GGCAGCCAAC
    GTGCCCCCCA GTGAGGCCCT GCAGCCCAAC AGCAGCCCCA GTGCCCGGCG GTGCCACCTC
    GAAGCCAAAT ACCGCGAGGG CAAGTACCGC CGTTACCACC CACTCTTTGG CAACCAGGAG
    GAGCTGGACA AGGCCCTGTG TGCCGCAGTC ACCACCTCAG ACCTGGCTGA GACCCAGGCA
    CTCCTGGGCT GTGGGGCTGG GGTCAACTGC TTCTCAGGGG ACCCCAAGGC CCCCACGCCC
    CTGGCTCTTG CCGAGCAGGC GGGACAGACA CTGCAGATGG AATTCCTTCG GAACAACCGG
    ACCACAGAGG CACCTCGGCT GGATTCAGTG AGGCCCCTGG AAAAGCACTA CTCGGTTATC
    CTGCCAACTG TGAGCCACAG CAGCTTCGTC TACAAGACTG CTTCTGCAGG GAAGCTGCTG
    CAGGACCGCC GGGCTCGGGA AGAGTTCAGC CGACGCTGGT GTGTGCTTAG CGACGGGGTC
    CTGAGCTACT ATGAGAATGA GCGGGCAGTG ACCCCCAACG GGGAGATTCG GGCCAGTGAG
    ATTGTGTGTC TGGCAGTGCC CACTCCTGAC ACCCATGGTT TTGAGCACAC CTTTGAGGTG
    TACACAGAAG GAGAGCGGCT ATACCTGTTT GGGCTGGAGA CCGCAGAGCA GGCTCGTGAG
    TGGATCAAGT GCATTGCCAA GGCGTTCGTG CCTCCCCTGG CTGAGGATCT GCTGGCTCGG
    GACTTTGAGA GGCTTGGACG CCTTCCCTAC AAAGCTGGCC TGAGCCTACA GCAGGCGCAG
    GAGGGCTGGT TTGCCCTCAC CGGCTCCGAG CTCTACGCTG TCTTCCCAGA AGGCCCCTGT
    GAGGAGCCAC TGCAACTTCG GAAATTACAG GAGCTTTCCG TCCAGGGGGA CAGCGAGAAC
    CAGGTGCTGG TGCTGGTGGA GCGACGGAGG ACGCTGTACA TCCAGGGGGA GCGGCGGCTG
    GATTTCATGG GCTGGCTAGG GGCCATCCAG AAAGCAGCGG CCAGCTCCGG GGACACATTG
    TCAGAGCAGC AGCTGGGAGA TTCGGATATC CCCGTGATTG TGTACCGCTG TGTGGACTAC
    ATCACGCAGT ACGGCCTGAC CTCGGAGGGC ATCTACCGCA AGTGTGGGCA GACGTCAAAA
    ACACTACGGC TGCTGGAGAG CCTACGGTTG GACGCTCGCT CTGTGCGCCT CAAGGAGGGC
    GAGCAGCACG TGGACGACGT CTCCTCCGCA CTCAAGCGCT TCTTGCGAGA TCTGCCCGAT
    GGGCTCTTCA CTCGAGTCCA GCGCCTAGCC TGGCTGGAGG CCTCAGAGAT TGAGGATGAG
    GAGGAGAAGG TGTCCAGGTA CCGAGAGCTT CTGGCACGTC TGCCCCCAGT CAACCGGGCC
    ACAGTGAAGG CTCTTATCAG CCACTTGTAC TGTGTCCAGT GCTTCTCAGA CACGAACCAG
    ATGAACACGC ACAACCTGGC TATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG
    GACTACAAGG CCGGCCGTGT GGTGGAAGAC CTCATCAGCC ACTATGTGGT GGTATTCAGT
    GTGGATGAGG AGGAGCTGAG GAAGCAGCGG GAGGAGGTCA CTGCCATCGT GAAGATGCGT
    GTGGCTGGCA CTGCTAGCGG GACCCAGCAC GCCGGTGACT TCATCTGTAC CGTGTACCTA
    GAGGAGAAGA AGGCGGAGGC AGAGCAACAC GTCAAGATCC CAGCATCCAT GACTGCAGAG
    GAGCTTACCT TGGAGATCCT GGATCGCAGG AATGTGGGCA TCAGGGAGAA GGACTATTGG
    ACCTGCTTCG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG
    GTGCTTCCCA TCCTGCACGG GCTGGGCACA GACAGCTACC TGGTGGTGAA GAAGCACCAG
    TCCATGGAGG CCATGCTGCT GTACCTAGCC AGCCACGTGG GTGAGACCAA GCACGGCATG
    ATGAAGTTCC GAGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCACGGG CGGCTTCCAC
    GATCGCTACT TCATCCTCAA CAGCAGCTGC CTGCGGCTCT ATAAGGAGGT CCGGAGCCAG
    AGGCCATGGA GCGGGGCCCC TGAGACCAGT CACCGGCCTG AGAAGGAGTG GCCTGTCAGG
    AGTCTCAAAG TCTACCTGGG AGTGAAGAAG AAACTCCGGC CGCCCACCTG CTGGGGCTTC
    ACGGTGATAC ACGAGACGGA GAAACACGAG AAGCAGCAGT GGTACCTGTG CTGTGAGACA
    CAGATGGAGC TCCGGGAGTG GCTCGCCACC TTCCTCTTCG TGCAGCACGA CGGCCTGGTG
    TGGCCCTCGG AGCCCTCACG CGTGTCCCGG GCAGCGCCTG AGGTCCGGCT GGGCAGCATT
    TCATTGATCC CCCTGCGAGG CAGTGAGAAT GAGATGCGCC GGAGTGTGGC CGCCTTTTCT
    GCGGACCCCC TTTCTCTTCT CCGAAACGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831637.1
    CDS596..4228
    Translation

    Target ORF information:

    RefSeq Version XM_024975869.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGACCAAGA AGGAGGAGCC CCCTGCGAGT CAAGTCCCAC GGGCCGTGCG TGTGGCCAGT 
    CTGCTGAGCG AAGGGGAGGA CCTGTCTGGA GATGACCAAG GGGATGAAGA TGAGGATGAC
    TATGCCTACG AGCGTGTCCC CAATGGTGAC TGGCACACCA GCAGCCTGAG CTCATCTTTG
    CCCAGCAGCC TCCTGACCCC TGAGCTTCCC CCGCACCCCA TGGACGGGCT GCCCGTGGGC
    CCCACACCCA CCCCACTGGT CATCAAGGCT GGCTGGCTGG ACAAGAACCC CCCGCAGGGA
    TCTTACATCT ATCAGAAGCG ATGGGTGAGA CTGGATGCCA ATCACCTGCG ATACTTTGAT
    AGTAACAAGG ATGCCTACTC AAAGCGCTTT GTCCCCGTGG CCTGCATCTC CAGAGTGGCT
    GCCATTGGGG ACCAAAAGTT CGAAGTGATC ACGAACAACC GGACCTTTGC CTTCCGGGCA
    GAGAGTGATG CGGAGCGGAA GGAGTGGATG CAGGCCCTGC AGCAGGCGGT GGCTGAGCAG
    CGTGCCCGGG CCCGCGTGTC TAGTGCTTAC CCATTGGGTG TTCAAGGCTC AGAGCCCCAT
    GACCATGCTG GCAGCCTGGA GCTACGTGGC TTCAAGAATA AACTCTATGT GGCCGTGGTC
    GGGGACAAAG TTGAGCTTTA CAAGAATCTG GAGGAGTACC GCCTGGGCAT CGGCATCACC
    TTCATTGACA TGAGTGTGGG CAACGTGAAG GAAGCGGCCG ACCGGCGCAG CTTCGACCTC
    ACCACCCCCT ACCGCATCTT CAGCTTCTCA GCCGAATCGG AGCTGGAGAA GGAGCAGTGG
    CTGGAGGCCA TGCAGGGAGC CATCGCCGAG GCCCTGTCCA CCTCGGAGGT GGCCGAGCGC
    ATCTGGGCCA TGGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTGCCCA GCCTGACTGG
    GCCTCCATCA ATCTCTGTGT TGTCATCTGT AAGCGCTGTG CAGGAGAGCA CCGTGGCCTG
    GGCGCTGGTA TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACGGAAACA
    CTCATCGAGC TCTTCTTACA GCTGGGCAAT GCTGCCGGGA ACCACTTCTG GGCAGCCAAC
    GTGCCCCCCA GTGAGGCCCT GCAGCCCAAC AGCAGCCCCA GTGCCCGGCG GTGCCACCTC
    GAAGCCAAAT ACCGCGAGGG CAAGTACCGC CGTTACCACC CACTCTTTGG CAACCAGGAG
    GAGCTGGACA AGGCCCTGTG TGCCGCAGTC ACCACCTCAG ACCTGGCTGA GACCCAGGCA
    CTCCTGGGCT GTGGGGCTGG GGTCAACTGC TTCTCAGGGG ACCCCAAGGC CCCCACGCCC
    CTGGCTCTTG CCGAGCAGGC GGGACAGACA CTGCAGATGG AATTCCTTCG GAACAACCGG
    ACCACAGAGG CACCTCGGCT GGATTCAGTG AGGCCCCTGG AAAAGCACTA CTCGGTTATC
    CTGCCAACTG TGAGCCACAG CAGCTTCGTC TACAAGACTG CTTCTGCAGG GAAGCTGCTG
    CAGGACCGCC GGGCTCGGGA AGAGTTCAGC CGACGCTGGT GTGTGCTTAG CGACGGGGTC
    CTGAGCTACT ATGAGAATGA GCGGGCAGTG ACCCCCAACG GGGAGATTCG GGCCAGTGAG
    ATTGTGTGTC TGGCAGTGCC CACTCCTGAC ACCCATGGTT TTGAGCACAC CTTTGAGGTG
    TACACAGAAG GAGAGCGGCT ATACCTGTTT GGGCTGGAGA CCGCAGAGCA GGCTCGTGAG
    TGGATCAAGT GCATTGCCAA GGCGTTCGTG CCTCCCCTGG CTGAGGATCT GCTGGCTCGG
    GACTTTGAGA GGCTTGGACG CCTTCCCTAC AAAGCTGGCC TGAGCCTACA GCAGGCGCAG
    GAGGGCTGGT TTGCCCTCAC CGGCTCCGAG CTCTACGCTG TCTTCCCAGA AGGCCCCTGT
    GAGGAGCCAC TGCAACTTCG GAAATTACAG GAGCTTTCCG TCCAGGGGGA CAGCGAGAAC
    CAGGTGCTGG TGCTGGTGGA GCGACGGAGG ACGCTGTACA TCCAGGGGGA GCGGCGGCTG
    GATTTCATGG GCTGGCTAGG GGCCATCCAG AAAGCAGCGG CCAGCTCCGG GGACACATTG
    TCAGAGCAGC AGCTGGGAGA TTCGGATATC CCCGTGATTG TGTACCGCTG TGTGGACTAC
    ATCACGCAGT ACGGCCTGAC CTCGGAGGGC ATCTACCGCA AGTGTGGGCA GACGTCAAAA
    ACACTACGGC TGCTGGAGAG CCTACGGTTG GACGCTCGCT CTGTGCGCCT CAAGGAGGGC
    GAGCAGCACG TGGACGACGT CTCCTCCGCA CTCAAGCGCT TCTTGCGAGA TCTGCCCGAT
    GGGCTCTTCA CTCGAGTCCA GCGCCTAGCC TGGCTGGAGG CCTCAGAGAT TGAGGATGAG
    GAGGAGAAGG TGTCCAGGTA CCGAGAGCTT CTGGCACGTC TGCCCCCAGT CAACCGGGCC
    ACAGTGAAGG CTCTTATCAG CCACTTGTAC TGTGTCCAGT GCTTCTCAGA CACGAACCAG
    ATGAACACGC ACAACCTGGC TATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG
    GACTACAAGG CCGGCCGTGT GGTGGAAGAC CTCATCAGCC ACTATGTGGT GGTATTCAGT
    GTGGATGAGG AGGAGCTGAG GAAGCAGCGG GAGGAGGTCA CTGCCATCGT GAAGATGCGT
    GTGGCTGGCA CTGCTAGCGG GACCCAGCAC GCCGGTGACT TCATCTGTAC CGTGTACCTA
    GAGGAGAAGA AGGCGGAGGC AGAGCAACAC GTCAAGATCC CAGCATCCAT GACTGCAGAG
    GAGCTTACCT TGGAGATCCT GGATCGCAGG AATGTGGGCA TCAGGGAGAA GGACTATTGG
    ACCTGCTTCG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG
    GTGCTTCCCA TCCTGCACGG GCTGGGCACA GACAGCTACC TGGTGGTGAA GAAGCACCAG
    TCCATGGAGG CCATGCTGCT GTACCTAGCC AGCCACGTGG GTGAGACCAA GCACGGCATG
    ATGAAGTTCC GAGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCACGGG CGGCTTCCAC
    GATCGCTACT TCATCCTCAA CAGCAGCTGC CTGCGGCTCT ATAAGGAGGT CCGGAGCCAG
    AGGCCATGGA GCGGGGCCCC TGAGACCAGT CACCGGCCTG AGAAGGAGTG GCCTGTCAGG
    AGTCTCAAAG TCTACCTGGG AGTGAAGAAG AAACTCCGGC CGCCCACCTG CTGGGGCTTC
    ACGGTGATAC ACGAGACGGA GAAACACGAG AAGCAGCAGT GGTACCTGTG CTGTGAGACA
    CAGATGGAGC TCCGGGAGTG GCTCGCCACC TTCCTCTTCG TGCAGCACGA CGGCCTGGTG
    TGGCCCTCGG AGCCCTCACG CGTGTCCCGG GCAGCGCCTG AGGTCCGGCT GGGCAGCATT
    TCATTGATCC CCCTGCGAGG CAGTGAGAAT GAGATGCGCC GGAGTGTGGC CGCCTTTTCT
    GCGGACCCCC TTTCTCTTCT CCGAAACGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223293
    Clone ID Related Accession (Same CDS sequence) XM_024975870.1
    Accession Version XM_024975870.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3600bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCAAGA AGGAGGAGCC CCCTGCGAGT CAAGTCCCAC GGGCCGTGCG TGTGGCCAGT 
    CTGCTGAGCG AAGGGGAGGA CCTGTCTGGA GATGACCAAG GGGATGAAGA TGAGGATGAC
    TATGCCTACG AGCGTGTCCC CAATGGTGAC TGGCACACCA GCAGCCTGAG CTCATCTTTG
    CCCAGCAGCC TCCTGACCCC TGAGCTTCCC CCGCACCCCA TGGACGGGCT GCCCGTGGGC
    CCCACACCCA CCCCACTGGT CATCAAGGCT GGCTGGCTGG ACAAGAACCC CCCGCAGGGA
    TCTTACATCT ATCAGAAGCG ATGGGTGAGA CTGGATGCCA ATCACCTGCG ATACTTTGAT
    AGTAACAAGG ATGCCTACTC AAAGCGCTTT GTCCCCGTGG CCTGCATCTC CAGAGTGGCT
    GCCATTGGGG ACCAAAAGTT CGAAGTGATC ACGAACAACC GGACCTTTGC CTTCCGGGCA
    GAGAGTGATG CGGAGCGGAA GGAGTGGATG CAGGCCCTGC AGCAGGCGGT GGCTGAGCAG
    CGTGCCCGGG CCCGCGTGTC TAGTGCTTAC CCATTGGGTG TTCAAGGCTC AGAGCCCCAT
    GACCATGCTG GCAGCCTGGA GCTACGTGGC TTCAAGAATA AACTCTATGT GGCCGTGGTC
    GGGGACAAAG TTGAGCTTTA CAAGAATCTG GAGGAGTACC GCCTGGGCAT CGGCATCACC
    TTCATTGACA TGAGTGTGGG CAACGTGAAG GAAGCGGCCG ACCGGCGCAG CTTCGACCTC
    ACCACCCCCT ACCGCATCTT CAGCTTCTCA GCCGAATCGG AGCTGGAGAA GGAGCAGTGG
    CTGGAGGCCA TGCAGGGAGC CATCGCCGAG GCCCTGTCCA CCTCGGAGGT GGCCGAGCGC
    ATCTGGGCCA TGGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTGCCCA GCCTGACTGG
    GCCTCCATCA ATCTCTGTGT TGTCATCTGT AAGCGCTGTG CAGGAGAGCA CCGTGGCCTG
    GGCGCTGGTA TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACGGAAACA
    CTCATCGAGC TCTTCTTACA GCTGGGCAAT GCTGCCGGGA ACCACTTCTG GGCAGCCAAC
    GTGCCCCCCA GTGAGGCCCT GCAGCCCAAC AGCAGCCCCA GTGCCCGGCG GTGCCACCTC
    GAAGCCAAAT ACCGCGAGGG CAAGTACCGC CGTTACCACC CACTCTTTGG CAACCAGGAG
    GAGCTGGACA AGGCCCTGTG TGCCGCAGTC ACCACCTCAG ACCTGGCTGA GACCCAGGCA
    CTCCTGGGCT GTGGGGCTGG GGTCAACTGC TTCTCAGGGG ACCCCAAGGC CCCCACGCCC
    CTGGCTCTTG CCGAGCAGGC GGGACAGACA CTGCAGATGG AATTCCTTCG GAACAACCGG
    ACCACAGAGG CACCTCGGCT GGATTCAGTG AGGCCCCTGG AAAAGCACTA CTCGGTTATC
    CTGCCAACTG TGAGCCACAG CAGCTTCGTC TACAAGACTG CTTCTGCAGG GAAGCTGCTG
    CAGGACCGCC GGGCTCGGGA AGAGTTCAGC CGACGCTGGT GTGTGCTTAG CGACGGGGTC
    CTGAGCTACT ATGAGAATGA GCGGGCAGTG ACCCCCAACG GGGAGATTCG GGCCAGTGAG
    ATTGTGTGTC TGGCAGTGCC CACTCCTGAC ACCCATGGTT TTGAGCACAC CTTTGAGGTG
    TACACAGAAG GAGAGCGGCT ATACCTGTTT GGGCTGGAGA CCGCAGAGCA GGCTCGTGAG
    TGGATCAAGT GCATTGCCAA GGCGTTCGTG CCTCCCCTGG CTGAGGATCT GCTGGCTCGG
    GACTTTGAGA GGCTTGGACG CCTTCCCTAC AAAGCTGGCC TGAGCCTACA GCAGGCGCAG
    GAGGGCTGGT TTGCCCTCAC CGGCTCCGAG CTCTACGCTG TCTTCCCAGA AGGCCCCTGT
    GAGGAGCCAC TGCAACTTCG GAAATTACAG GAGCTTTCCG TCCAGGGGGA CAGCGAGAAC
    CAGGTGCTGG TGCTGGTGGA GCGACGGAGG ACGCTGTACA TCCAGGGGGA GCGGCGGCTG
    GATTTCATGG GCTGGCTAGG GGCCATCCAG AAAGCAGCGG CCAGCTCCGG GGACACATTG
    TCAGAGCAGC AGCTGGGAGA TTCGGATATC CCCGTGATTG TGTACCGCTG TGTGGACTAC
    ATCACGCAGT ACGGCCTGAC CTCGGAGGGC ATCTACCGCA AGTGTGGGCA GACGTCAAAA
    ACACTACGGC TGCTGGAGAG CCTACGGTTG GACGCTCGCT CTGTGCGCCT CAAGGAGGGC
    GAGCAGCACG TGGACGACGT CTCCTCCGCA CTCAAGCGCT TCTTGCGAGA TCTGCCCGAT
    GGGCTCTTCA CTCGAGTCCA GCGCCTAGCC TGGCTGGAGG CCTCAGAGAT TGAGGATGAG
    GAGGAGAAGG TGTCCAGGTA CCGAGAGCTT CTGGCACGTC TGCCCCCAGT CAACCGGGCC
    ACAGTGAAGG CTCTTATCAG CCACTTGTAC TGTGTCCAGT GCTTCTCAGA CACGAACCAG
    ATGAACACGC ACAACCTGGC TATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG
    GACTACAAGG CCGGCCGTGT GGTGGAAGAC CTCATCAGCC ACTATGTGGT GGTATTCAGT
    GTGGATGAGG AGGAGCTGAG GAAGCAGCGG GAGGAGGTCA CTGCCATCGT GAAGATGCGT
    GTGGCTGGCA CTGCTAGCGG GACCCAGCAC GCCGGTGACT TCATCTGTAC CGTGTACCTA
    GAGGAGAAGA AGGCGGAGGC AGAGCAACAC GTCAAGATCC CAGCATCCAT GACTGCAGAG
    GAGCTTACCT TGGAGATCCT GGATCGCAGG AATGTGGGCA TCAGGGAGAA GGACTATTGG
    ACCTGCTTCG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG
    GTGCTTCCCA TCCTGCACGG GCTGGGCACA GACAGCTACC TGGTGGTGAA GAAGCACCAG
    TCCATGGAGG CCATGCTGCT GTACCTAGCC AGCCACGTGG GTGAGACCAA GCACGGCATG
    ATGAAGTTCC GAGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCACGGG CGGCTTCCAC
    GATCGCTACT TCATCCTCAA CAGCAGCTGC CTGCGGCTCT ATAAGGAGGT CCGGAGTCAC
    CGGCCTGAGA AGGAGTGGCC TGTCAGGAGT CTCAAAGTCT ACCTGGGAGT GAAGAAGAAA
    CTCCGGCCGC CCACCTGCTG GGGCTTCACG GTGATACACG AGACGGAGAA ACACGAGAAG
    CAGCAGTGGT ACCTGTGCTG TGAGACACAG ATGGAGCTCC GGGAGTGGCT CGCCACCTTC
    CTCTTCGTGC AGCACGACGG CCTGGTGTGG CCCTCGGAGC CCTCACGCGT GTCCCGGGCA
    GCGCCTGAGG TCCGGCTGGG CAGCATTTCA TTGATCCCCC TGCGAGGCAG TGAGAATGAG
    ATGCGCCGGA GTGTGGCCGC CTTTTCTGCG GACCCCCTTT CTCTTCTCCG AAACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024831638.1
    CDS639..4238
    Translation

    Target ORF information:

    RefSeq Version XM_024975870.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024975870.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGACCAAGA AGGAGGAGCC CCCTGCGAGT CAAGTCCCAC GGGCCGTGCG TGTGGCCAGT 
    CTGCTGAGCG AAGGGGAGGA CCTGTCTGGA GATGACCAAG GGGATGAAGA TGAGGATGAC
    TATGCCTACG AGCGTGTCCC CAATGGTGAC TGGCACACCA GCAGCCTGAG CTCATCTTTG
    CCCAGCAGCC TCCTGACCCC TGAGCTTCCC CCGCACCCCA TGGACGGGCT GCCCGTGGGC
    CCCACACCCA CCCCACTGGT CATCAAGGCT GGCTGGCTGG ACAAGAACCC CCCGCAGGGA
    TCTTACATCT ATCAGAAGCG ATGGGTGAGA CTGGATGCCA ATCACCTGCG ATACTTTGAT
    AGTAACAAGG ATGCCTACTC AAAGCGCTTT GTCCCCGTGG CCTGCATCTC CAGAGTGGCT
    GCCATTGGGG ACCAAAAGTT CGAAGTGATC ACGAACAACC GGACCTTTGC CTTCCGGGCA
    GAGAGTGATG CGGAGCGGAA GGAGTGGATG CAGGCCCTGC AGCAGGCGGT GGCTGAGCAG
    CGTGCCCGGG CCCGCGTGTC TAGTGCTTAC CCATTGGGTG TTCAAGGCTC AGAGCCCCAT
    GACCATGCTG GCAGCCTGGA GCTACGTGGC TTCAAGAATA AACTCTATGT GGCCGTGGTC
    GGGGACAAAG TTGAGCTTTA CAAGAATCTG GAGGAGTACC GCCTGGGCAT CGGCATCACC
    TTCATTGACA TGAGTGTGGG CAACGTGAAG GAAGCGGCCG ACCGGCGCAG CTTCGACCTC
    ACCACCCCCT ACCGCATCTT CAGCTTCTCA GCCGAATCGG AGCTGGAGAA GGAGCAGTGG
    CTGGAGGCCA TGCAGGGAGC CATCGCCGAG GCCCTGTCCA CCTCGGAGGT GGCCGAGCGC
    ATCTGGGCCA TGGCCCCCAA CAGGTTCTGT GCTGACTGCG GGGCTGCCCA GCCTGACTGG
    GCCTCCATCA ATCTCTGTGT TGTCATCTGT AAGCGCTGTG CAGGAGAGCA CCGTGGCCTG
    GGCGCTGGTA TCTCCAAGGT GCGGAGCCTG AAGATGGACA GGAAGGTGTG GACGGAAACA
    CTCATCGAGC TCTTCTTACA GCTGGGCAAT GCTGCCGGGA ACCACTTCTG GGCAGCCAAC
    GTGCCCCCCA GTGAGGCCCT GCAGCCCAAC AGCAGCCCCA GTGCCCGGCG GTGCCACCTC
    GAAGCCAAAT ACCGCGAGGG CAAGTACCGC CGTTACCACC CACTCTTTGG CAACCAGGAG
    GAGCTGGACA AGGCCCTGTG TGCCGCAGTC ACCACCTCAG ACCTGGCTGA GACCCAGGCA
    CTCCTGGGCT GTGGGGCTGG GGTCAACTGC TTCTCAGGGG ACCCCAAGGC CCCCACGCCC
    CTGGCTCTTG CCGAGCAGGC GGGACAGACA CTGCAGATGG AATTCCTTCG GAACAACCGG
    ACCACAGAGG CACCTCGGCT GGATTCAGTG AGGCCCCTGG AAAAGCACTA CTCGGTTATC
    CTGCCAACTG TGAGCCACAG CAGCTTCGTC TACAAGACTG CTTCTGCAGG GAAGCTGCTG
    CAGGACCGCC GGGCTCGGGA AGAGTTCAGC CGACGCTGGT GTGTGCTTAG CGACGGGGTC
    CTGAGCTACT ATGAGAATGA GCGGGCAGTG ACCCCCAACG GGGAGATTCG GGCCAGTGAG
    ATTGTGTGTC TGGCAGTGCC CACTCCTGAC ACCCATGGTT TTGAGCACAC CTTTGAGGTG
    TACACAGAAG GAGAGCGGCT ATACCTGTTT GGGCTGGAGA CCGCAGAGCA GGCTCGTGAG
    TGGATCAAGT GCATTGCCAA GGCGTTCGTG CCTCCCCTGG CTGAGGATCT GCTGGCTCGG
    GACTTTGAGA GGCTTGGACG CCTTCCCTAC AAAGCTGGCC TGAGCCTACA GCAGGCGCAG
    GAGGGCTGGT TTGCCCTCAC CGGCTCCGAG CTCTACGCTG TCTTCCCAGA AGGCCCCTGT
    GAGGAGCCAC TGCAACTTCG GAAATTACAG GAGCTTTCCG TCCAGGGGGA CAGCGAGAAC
    CAGGTGCTGG TGCTGGTGGA GCGACGGAGG ACGCTGTACA TCCAGGGGGA GCGGCGGCTG
    GATTTCATGG GCTGGCTAGG GGCCATCCAG AAAGCAGCGG CCAGCTCCGG GGACACATTG
    TCAGAGCAGC AGCTGGGAGA TTCGGATATC CCCGTGATTG TGTACCGCTG TGTGGACTAC
    ATCACGCAGT ACGGCCTGAC CTCGGAGGGC ATCTACCGCA AGTGTGGGCA GACGTCAAAA
    ACACTACGGC TGCTGGAGAG CCTACGGTTG GACGCTCGCT CTGTGCGCCT CAAGGAGGGC
    GAGCAGCACG TGGACGACGT CTCCTCCGCA CTCAAGCGCT TCTTGCGAGA TCTGCCCGAT
    GGGCTCTTCA CTCGAGTCCA GCGCCTAGCC TGGCTGGAGG CCTCAGAGAT TGAGGATGAG
    GAGGAGAAGG TGTCCAGGTA CCGAGAGCTT CTGGCACGTC TGCCCCCAGT CAACCGGGCC
    ACAGTGAAGG CTCTTATCAG CCACTTGTAC TGTGTCCAGT GCTTCTCAGA CACGAACCAG
    ATGAACACGC ACAACCTGGC TATTGTGTTT GGGCCCACGC TCTTCCAGAC AGATGGGCAG
    GACTACAAGG CCGGCCGTGT GGTGGAAGAC CTCATCAGCC ACTATGTGGT GGTATTCAGT
    GTGGATGAGG AGGAGCTGAG GAAGCAGCGG GAGGAGGTCA CTGCCATCGT GAAGATGCGT
    GTGGCTGGCA CTGCTAGCGG GACCCAGCAC GCCGGTGACT TCATCTGTAC CGTGTACCTA
    GAGGAGAAGA AGGCGGAGGC AGAGCAACAC GTCAAGATCC CAGCATCCAT GACTGCAGAG
    GAGCTTACCT TGGAGATCCT GGATCGCAGG AATGTGGGCA TCAGGGAGAA GGACTATTGG
    ACCTGCTTCG AGGTCAACGA GAGGGAGGAG GCAGAGCGCC CCCTGCACTT TGCGGAGAAG
    GTGCTTCCCA TCCTGCACGG GCTGGGCACA GACAGCTACC TGGTGGTGAA GAAGCACCAG
    TCCATGGAGG CCATGCTGCT GTACCTAGCC AGCCACGTGG GTGAGACCAA GCACGGCATG
    ATGAAGTTCC GAGAGGACCG CAGCCTCCTG GGCCTGGGCC TGCCCACGGG CGGCTTCCAC
    GATCGCTACT TCATCCTCAA CAGCAGCTGC CTGCGGCTCT ATAAGGAGGT CCGGAGTCAC
    CGGCCTGAGA AGGAGTGGCC TGTCAGGAGT CTCAAAGTCT ACCTGGGAGT GAAGAAGAAA
    CTCCGGCCGC CCACCTGCTG GGGCTTCACG GTGATACACG AGACGGAGAA ACACGAGAAG
    CAGCAGTGGT ACCTGTGCTG TGAGACACAG ATGGAGCTCC GGGAGTGGCT CGCCACCTTC
    CTCTTCGTGC AGCACGACGG CCTGGTGTGG CCCTCGGAGC CCTCACGCGT GTCCCGGGCA
    GCGCCTGAGG TCCGGCTGGG CAGCATTTCA TTGATCCCCC TGCGAGGCAG TGAGAATGAG
    ATGCGCCGGA GTGTGGCCGC CTTTTCTGCG GACCCCCTTT CTCTTCTCCG AAACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92731
    Clone ID Related Accession (Same CDS sequence) XM_015474774.1 , XM_005194645.3
    Accession Version XM_015474774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104415.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCATGC CCTGCTTCTT
    TTGTTGGCTG AGCATCGTGC CCCGGCCCGT TTGTCGAGTG CTTACCCATT GGGTGTTCAT
    GGCTCAGATC CCCTTGACCA TGCTGGCTTC CTGGAACTAC ATGGCTTCTA GAATAAACTC
    TATGTTGTTG TGGTCTCGGA TTAAGTTGAT CTTTACAAGA ATCTGGACGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015330260.1
    CDS253..4620
    Translation

    Target ORF information:

    RefSeq Version XM_015474774.1
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCATGC CCTGCTTCTT
    TTGTTGGCTG AGCATCGTGC CCCGGCCCGT TTGTCGAGTG CTTACCCATT GGGTGTTCAT
    GGCTCAGATC CCCTTGACCA TGCTGGCTTC CTGGAACTAC ATGGCTTCTA GAATAAACTC
    TATGTTGTTG TGGTCTCGGA TTAAGTTGAT CTTTACAAGA ATCTGGACGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92731
    Clone ID Related Accession (Same CDS sequence) XM_015474774.1 , XM_005194645.3
    Accession Version XM_005194645.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4368bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product LOW QUALITY PROTEIN: arf-GAP with Rho-GAP domain, ANK repeat and PH domain-containing protein 1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646268.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005194645.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## frameshifts :: corrected 2 indels internal stop codons :: corrected 2 genomic stop codons ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCATGC CCTGCTTCTT
    TTGTTGGCTG AGCATCGTGC CCCGGCCCGT TTGTCGAGTG CTTACCCATT GGGTGTTCAT
    GGCTCAGATC CCCTTGACCA TGCTGGCTTC CTGGAACTAC ATGGCTTCTA GAATAAACTC
    TATGTTGTTG TGGTCTCGGA TTAAGTTGAT CTTTACAAGA ATCTGGACGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005194702.2
    CDS253..4620
    Translation

    Target ORF information:

    RefSeq Version XM_005194645.3
    Organism Bos taurus(cattle)
    Definition Bos taurus ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 1 (ARAP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005194645.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGCGGAGG CCGGGGACGG GGCGCTCTCA GTGGCTGAGT GGCTGCGAGC GCTGCACCTG 
    GAGCAGTACA CGAGGCTCTT CGAGCAGCAC GGCCTGGTGT GGGCCACAGA CTGCCAAGGC
    CTCAGCGACA CCCGCCTGGT AGACATGGGC ATGGTGCTCC CTGGCCACCG CCGTCGCATC
    CTGGCTGGCC TGCTCCGTGC CCACACACCC CCAGCCCCTG CACCCCGCCT GGCCCCACGG
    CCCGTGCCCA TGAAGCGTCA TGTCTTCCGC TCACCACCCA CGCCCACCAC TCCCCCGGAG
    CCACTGCCTG CGGCTGGAGA GGACGAGGGG CTGCCCACCG CCCCACCCAT CCCGCCCCGG
    AAGAGCTGCC TTCCGCCCAC CTGCTTCTCC GCCCCGTCCA CAGTGGCCCC AGACCCTGTG
    GTGCCCCCAT TGCCCGCCAA GCGGCATTTG ACAGAGCTCA GCCTTCCGCC CGTGCCCCCC
    CGCACTGGGC ACCCCCGCCC ACTGGTGAGC CTTCCCTCCA AGGAAGAGGA GGTACTATCA
    CCACCATCAC CGCCCCCTCC CCAACTGGAG CCTGCGGAGC CCCTGGCCAC TTTCCCCAGG
    GGGCCTCCTC AGCCCCTGTC TGCACCTTGC AGCCCCCCAG AGGTCCCTCC GAAGCCCCAC
    CGCCTGCCCC CGGAGTTTGA TGACTCTGAC TACGATGAGG TCCCAGAGGA GGGGCCGAGG
    GCCCCAGCTG CCGTGATGAC CAAGAAGGAG GAGCCCCCTG CGAGTCAAGT CCCACGGGCC
    GTGCGTGTGG CCAGTCTGCT GAGCGAAGGG GAGGACCTGT CTGGAGATGA CCAAGGGGAT
    GAAGATGAGG ATGACTATGC CTACGAGCGT GTCCCCAATG GTGACTGGCA CACCAGCAGC
    CTGAGCTCAT CTTTGCCCAG CAGCCTCCTG ACCCCTGAGC TTCCCCCGCA CCCCATGGAC
    GGGCTGCCCG TGGGCCCCAC ACCCACCCCA CTGGTCATCA AGGCTGGCTG GCTGGACAAG
    AACCCCCCGC AGGGATCTTA CATCTATCAG AAGCGATGGG TGAGACTGGA TGCCAATCAC
    CTGCGATACT TTGATAGTAA CAAGGATGCC TACTCAAAGC GCTTTGTCCC CGTGGCCTGC
    ATCTCCAGAG TGGCTGCCAT TGGGGACCAA AAGTTCGAAG TGATCACGAA CAACCGGACC
    TTTGCCTTCC GGGCAGAGAG TGATGCGGAG CGGAAGGAGT GGATGCATGC CCTGCTTCTT
    TTGTTGGCTG AGCATCGTGC CCCGGCCCGT TTGTCGAGTG CTTACCCATT GGGTGTTCAT
    GGCTCAGATC CCCTTGACCA TGCTGGCTTC CTGGAACTAC ATGGCTTCTA GAATAAACTC
    TATGTTGTTG TGGTCTCGGA TTAAGTTGAT CTTTACAAGA ATCTGGACGA GTACCGCCTG
    GGCATCGGCA TCACCTTCAT TGACATGAGT GTGGGCAACG TGAAGGAAGC GGCCGACCGG
    CGCAGCTTCG ACCTCACCAC CCCCTACCGC ATCTTCAGCT TCTCAGCCGA ATCGGAGCTG
    GAGAAGGAGC AGTGGCTGGA GGCCATGCAG GGAGCCATCG CCGAGGCCCT GTCCACCTCG
    GAGGTGGCCG AGCGCATCTG GGCCATGGCC CCCAACAGGT TCTGTGCTGA CTGCGGGGCT
    GCCCAGCCTG ACTGGGCCTC CATCAATCTC TGTGTTGTCA TCTGTAAGCG CTGTGCAGGA
    GAGCACCGTG GCCTGGGCGC TGGTATCTCC AAGGTGCGGA GCCTGAAGAT GGACAGGAAG
    GTGTGGACGG AAACACTCAT CGAGCTCTTC TTACAGCTGG GCAATGCTGC CGGGAACCAC
    TTCTGGGCAG CCAACGTGCC CCCCAGTGAG GCCCTGCAGC CCAACAGCAG CCCCAGTGCC
    CGGCGGTGCC ACCTCGAAGC CAAATACCGC GAGGGCAAGT ACCGCCGTTA CCACCCACTC
    TTTGGCAACC AGGAGGAGCT GGACAAGGCC CTGTGTGCCG CAGTCACCAC CTCAGACCTG
    GCTGAGACCC AGGCACTCCT GGGCTGTGGG GCTGGGGTCA ACTGCTTCTC AGGGGACCCC
    AAGGCCCCCA CGCCCCTGGC TCTTGCCGAG CAGGCGGGAC AGACACTGCA GATGGAATTC
    CTTCGGAACA ACCGGACCAC AGAGGCACCT CGGCTGGATT CAGTGAGGCC CCTGGAAAAG
    CACTACTCGG TTATCCTGCC AACTGTGAGC CACAGCAGCT TCGTCTACAA GACTGCTTCT
    GCAGGGAAGC TGCTGCAGGA CCGCCGGGCT CGGGAAGAGT TCAGCCGACG CTGGTGTGTG
    CTTAGCGACG GGGTCCTGAG CTACTATGAG AATGAGCGGG CAGTGACCCC CAACGGGGAG
    ATTCGGGCCA GTGAGATTGT GTGTCTGGCA GTGCCCACTC CTGACACCCA TGGTTTTGAG
    CACACCTTTG AGGTGTACAC AGAAGGAGAG CGGCTATACC TGTTTGGGCT GGAGACCGCA
    GAGCAGGCTC GTGAGTGGAT CAAGTGCATT GCCAAGGCGT TCGTGCCTCC CCTGGCTGAG
    GATCTGCTGG CTCGGGACTT TGAGAGGCTT GGACGCCTTC CCTACAAAGC TGGCCTGAGC
    CTACAGCAGG CGCAGGAGGG CTGGTTTGCC CTCACCGGCT CCGAGCTCTA CGCTGTCTTC
    CCAGAAGGCC CCTGTGAGGA GCCACTGCAA CTTCGGAAAT TACAGGAGCT TTCCGTCCAG
    GGGGACAGCG AGAACCAGGT GCTGGTGCTG GTGGAGCGAC GGAGGACGCT GTACATCCAG
    GGGGAGCGGC GGCTGGATTT CATGGGCTGG CTAGGGGCCA TCCAGAAAGC AGCGGCCAGC
    TCCGGGGACA CATTGTCAGA GCAGCAGCTG GGAGATTCGG ATATCCCCGT GATTGTGTAC
    CGCTGTGTGG ACTACATCAC GCAGTACGGC CTGACCTCGG AGGGCATCTA CCGCAAGTGT
    GGGCAGACGT CAAAAACACT ACGGCTGCTG GAGAGCCTAC GGTTGGACGC TCGCTCTGTG
    CGCCTCAAGG AGGGCGAGCA GCACGTGGAC GACGTCTCCT CCGCACTCAA GCGCTTCTTG
    CGAGATCTGC CCGATGGGCT CTTCACTCGA GTCCAGCGCC TAGCCTGGCT GGAGGCCTCA
    GAGATTGAGG ATGAGGAGGA GAAGGTGTCC AGGTACCGAG AGCTTCTGGC ACGTCTGCCC
    CCAGTCAACC GGGCCACAGT GAAGGCTCTT ATCAGCCACT TGTACTGTGT CCAGTGCTTC
    TCAGACACGA ACCAGATGAA CACGCACAAC CTGGCTATTG TGTTTGGGCC CACGCTCTTC
    CAGACAGATG GGCAGGACTA CAAGGCCGGC CGTGTGGTGG AAGACCTCAT CAGCCACTAT
    GTGGTGGTAT TCAGTGTGGA TGAGGAGGAG CTGAGGAAGC AGCGGGAGGA GGTCACTGCC
    ATCGTGAAGA TGCGTGTGGC TGGCACTGCT AGCGGGACCC AGCACGCCGG TGACTTCATC
    TGTACCGTGT ACCTAGAGGA GAAGAAGGCG GAGGCAGAGC AACACGTCAA GATCCCAGCA
    TCCATGACTG CAGAGGAGCT TACCTTGGAG ATCCTGGATC GCAGGAATGT GGGCATCAGG
    GAGAAGGACT ATTGGACCTG CTTCGAGGTC AACGAGAGGG AGGAGGCAGA GCGCCCCCTG
    CACTTTGCGG AGAAGGTGCT TCCCATCCTG CACGGGCTGG GCACAGACAG CTACCTGGTG
    GTGAAGAAGC ACCAGTCCAT GGAGGCCATG CTGCTGTACC TAGCCAGCCA CGTGGGTGAG
    ACCAAGCACG GCATGATGAA GTTCCGAGAG GACCGCAGCC TCCTGGGCCT GGGCCTGCCC
    ACGGGCGGCT TCCACGATCG CTACTTCATC CTCAACAGCA GCTGCCTGCG GCTCTATAAG
    GAGGTCCGGA GCCAGAGGCC ATGGAGCGGG GCCCCTGAGA CCAGTCACCG GCCTGAGAAG
    GAGTGGCCTG TCAGGAGTCT CAAAGTCTAC CTGGGAGTGA AGAAGAAACT CCGGCCGCCC
    ACCTGCTGGG GCTTCACGGT GATACACGAG ACGGAGAAAC ACGAGAAGCA GCAGTGGTAC
    CTGTGCTGTG AGACACAGAT GGAGCTCCGG GAGTGGCTCG CCACCTTCCT CTTCGTGCAG
    CACGACGGCC TGGTGTGGCC CTCGGAGCCC TCACGCGTGT CCCGGGCAGC GCCTGAGGTC
    CGGCTGGGCA GCATTTCATT GATCCCCCTG CGAGGCAGTG AGAATGAGAT GCGCCGGAGT
    GTGGCCGCCT TTTCTGCGGA CCCCCTTTCT CTTCTCCGAA ACGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.