COL4A5 cDNA ORF clone, Bos taurus(cattle)

The following COL4A5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa238554 XM_024988380.1
Latest version!
Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa35387 XM_002699860.4
Latest version!
Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35387 XM_001790133.6
Latest version!
Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35388 XM_001790134.6
Latest version!
Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35388 XM_002699862.5
Latest version!
Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OBa35388 XM_002699862.4 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa35387 XM_002699860.3 Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa238554
    Clone ID Related Accession (Same CDS sequence) XM_024988380.1
    Accession Version XM_024988380.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT
    GGGGCTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT
    TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC
    AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT
    CCCCCTGGAT TACCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA
    CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT
    GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG
    GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT
    ATTGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA
    CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA
    GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC
    AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT
    TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC
    TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GTCAGACATG
    TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024844148.1
    CDS335..5401
    Translation

    Target ORF information:

    RefSeq Version XM_024988380.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024988380.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTTTTCAAGG TCCACCAGGT CCAGAAGGTC CCCGGGGTCT ACCTGGAAGT
    GGGGCTATTA AAGGAGAAAG AGGTAACCCA GGCCAACCTG GGCAACCTGG TTTGCCTGGT
    TTGAAAGGTG ATCAAGGTCC TCCAGGACTC CAGGGTAACC CTGGCCGGCC AGGTCTCAAT
    GGAATGAAAG GAGATCCTGG TCTCCCTGGT GTTCCAGGAT TTCCAGGCAT GAAAGGACCC
    AGTGGAGTAC CTGGTTCAAC TGGCCCTGAG GGGGATCCAG GACTTATTGG CCCACCAGGT
    CCCCCTGGAT TACCTGGTCC TTCAGGACAG AGTATTGTAA TCAAAGGAGA TGCTGGCCCA
    CCAGGGATTC CAGGCCAGCC TGGATTAAAA GGTTTACAAG GACTCCCAGG ACCTCAGGGT
    TTACCAGGTC CAATTGGCCC TCCAGGAGAT CCTGGACGCA ATGGACTCCC TGGCTTTGAT
    GGTGCAGGAG GACGCAAAGG AGACCCAGGT TTGCCAGGCC AGCCAGGTAC TCGTGGCTTG
    GATGGTCCCC CCGGGCCAGA TGGATTGCAG GGTCCTCCAG GTCCCCCTGG AACCTCTTCT
    ATTGCCCATG GGTTTCTCAT CACACGTCAC AGCCAGACAA CAGATGCTCC ACAATGCCCA
    CAGGGAACCA TCCAGGTGTA TGAAGGCTTT TCTCTCCTGT ATGTACAAGG AAATAAAAGA
    GCTCATGGTC AAGACTTGGG AACAGCCGGC AGCTGCCTTC GTCGTTTCAG TACCATGCCT
    TTCATGTTCT GCAACATCAA TAATGTTTGC AACTTTGCTT CAAGAAATGA TTATTCTTAC
    TGGCTCTCCA CCCCAGAGCC CATGCCAATG AGCATGGAAC CCCTGAAGGG GCAGAGCATC
    CAGCCATTCA TTAGTCGATG TGCAGTATGT GAAGCTCCGG CCGTGGTGAT TGCAGTCCAC
    AGTCAGACCA TCCAGATTCC CCGTTGTCCT CAGGGATGGG ATTCTCTCTG GATTGGTTAT
    TCCTTCATGA TGCACACAAG TGCAGGGGCA GAGGGCTCAG GCCAAGCCCT GGCCTCCCCT
    GGTTCCTGCT TAGAAGAGTT TCGTTCAGCT CCCTTCATTG AGTGTCATGG GCGGGGGACC
    TGCAACTATT ACGCCAACTC CTACAGCTTT TGGCTGGCGA CTGTAGATGT GTCAGACATG
    TTCAGCAAAC CTCAATCAGA AACGCTGAAA GCAGGAGACT TGAGGACACG TATTAGCCGA
    TGTCAAGTGT GCATGAAGAG GACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35387
    Clone ID Related Accession (Same CDS sequence) XM_002699860.4 , XM_001790133.6 , XM_002699860.3
    Accession Version XM_002699860.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699860.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699906.1
    CDS373..5448
    Translation

    Target ORF information:

    RefSeq Version XM_002699860.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699860.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35387
    Clone ID Related Accession (Same CDS sequence) XM_002699860.4 , XM_001790133.6 , XM_002699860.3
    Accession Version XM_001790133.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790133.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001790185.2
    CDS31..5106
    Translation

    Target ORF information:

    RefSeq Version XM_001790133.6
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001790133.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35387
    Clone ID Related Accession (Same CDS sequence) XM_002699860.4 , XM_001790133.6 , XM_002699860.3
    Accession Version XM_002699860.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 5, 2013 this sequence version replaced XM_002699860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699906.1
    CDS31..5106
    Translation

    Target ORF information:

    RefSeq Version XM_002699860.3
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699860.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCTACAGG TTTTCAAGGT CCACCAGGTC CAGAAGGTCC CCGGGGTCTA
    CCTGGAAGTG GGGCTATTAA AGGAGAAAGA GGTAACCCAG GCCAACCTGG GCAACCTGGT
    TTGCCTGGTT TGAAAGGTGA TCAAGGTCCT CCAGGACTCC AGGGTAACCC TGGCCGGCCA
    GGTCTCAATG GAATGAAAGG AGATCCTGGT CTCCCTGGTG TTCCAGGATT TCCAGGCATG
    AAAGGACCCA GTGGAGTACC TGGTTCAACT GGCCCTGAGG GGGATCCAGG ACTTATTGGC
    CCACCAGGTC CCCCTGGATT ACCTGGTCCT TCAGGACAGA GTATTGTAAT CAAAGGAGAT
    GCTGGCCCAC CAGGGATTCC AGGCCAGCCT GGATTAAAAG GTTTACAAGG ACTCCCAGGA
    CCTCAGGGTT TACCAGGTCC AATTGGCCCT CCAGGAGATC CTGGACGCAA TGGACTCCCT
    GGCTTTGATG GTGCAGGAGG ACGCAAAGGA GACCCAGGTT TGCCAGGCCA GCCAGGTACT
    CGTGGCTTGG ATGGTCCCCC CGGGCCAGAT GGATTGCAGG GTCCTCCAGG TCCCCCTGGA
    ACCTCTTCTA TTGCCCATGG GTTTCTCATC ACACGTCACA GCCAGACAAC AGATGCTCCA
    CAATGCCCAC AGGGAACCAT CCAGGTGTAT GAAGGCTTTT CTCTCCTGTA TGTACAAGGA
    AATAAAAGAG CTCATGGTCA AGACTTGGGA ACAGCCGGCA GCTGCCTTCG TCGTTTCAGT
    ACCATGCCTT TCATGTTCTG CAACATCAAT AATGTTTGCA ACTTTGCTTC AAGAAATGAT
    TATTCTTACT GGCTCTCCAC CCCAGAGCCC ATGCCAATGA GCATGGAACC CCTGAAGGGG
    CAGAGCATCC AGCCATTCAT TAGTCGATGT GCAGTATGTG AAGCTCCGGC CGTGGTGATT
    GCAGTCCACA GTCAGACCAT CCAGATTCCC CGTTGTCCTC AGGGATGGGA TTCTCTCTGG
    ATTGGTTATT CCTTCATGAT GCACACAAGT GCAGGGGCAG AGGGCTCAGG CCAAGCCCTG
    GCCTCCCCTG GTTCCTGCTT AGAAGAGTTT CGTTCAGCTC CCTTCATTGA GTGTCATGGG
    CGGGGGACCT GCAACTATTA CGCCAACTCC TACAGCTTTT GGCTGGCGAC TGTAGATGTG
    TCAGACATGT TCAGCAAACC TCAATCAGAA ACGCTGAAAG CAGGAGACTT GAGGACACGT
    ATTAGCCGAT GTCAAGTGTG CATGAAGAGG ACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35388
    Clone ID Related Accession (Same CDS sequence) XM_002699862.4 , XM_002699862.5 , XM_001790134.6
    Accession Version XM_002699862.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104715.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002699862.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699908.1
    CDS31..5088
    Translation

    Target ORF information:

    RefSeq Version XM_002699862.4
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699862.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35388
    Clone ID Related Accession (Same CDS sequence) XM_002699862.4 , XM_002699862.5 , XM_001790134.6
    Accession Version XM_002699862.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002699862.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002699908.1
    CDS335..5392
    Translation

    Target ORF information:

    RefSeq Version XM_002699862.5
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 chain (COL4A5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002699862.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa35388
    Clone ID Related Accession (Same CDS sequence) XM_002699862.4 , XM_002699862.5 , XM_001790134.6
    Accession Version XM_001790134.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-5(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014647087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790134.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001790186.2
    CDS31..5088
    Translation

    Target ORF information:

    RefSeq Version XM_001790134.6
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 5 (COL4A5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001790134.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGAAACTGC GTGGAGTCAG CCTGGCTGCC GGCTTGTCCT TGCTGGCCCT GAGTCTTTGG 
    GGGCAGCCCG CAGAGGCTGC GGCTTGCTAT GGGTGTTCTC CAGGATCAAA GTGTGACTGT
    AGTGGTATAA AAGGAGAAAA GGGGGAGAGA GGATTTCCAG GTTTGGAAGG CCACCCAGGT
    TTGCCTGGAT TTCCAGGTCC AGAAGGGCCC CCAGGGCCTC GGGGACAAAA GGGTGATGAT
    GGAATTCCAG GGCCACCAGG ACCAAAAGGG ATCAGAGGTC CTCCTGGACT TCCTGGATTT
    CCAGGGACAC CAGGTCTTCC TGGAATGCCA GGCCATGATG GGGCCCCAGG ACCTCAAGGT
    ATCCCTGGAT GCAATGGAAC CAAGGGAGAA CGTGGATTTC CAGGCAGTCC CGGTTTTCCT
    GGTTTACAGG GTCCTCCAGG ACCTCCTGGG ATCCCAGGTA TGAAGGGAGA ACCGGGTAGT
    ATAATTATGT CATCCCTGCC AGGACCAAAG GGTAATCCAG GCTATCCAGG TCCTCCTGGA
    ATTCAAGGCC CAGCTGGTCC CCCTGGTATA CCAGGGCCAA TTGGTCCCCC AGGACCACCA
    GGTTTGATGG GCCCTCCTGG CCCACCAGGA CTTCCAGGAC CAAAGGGGAA TATGGGCTTA
    AATTTCCAGG GACCCAAGGG TGAGAAGGGT GAGCAAGGTC TTCAGGGCCC CCCTGGGCCA
    CCTGGGCAGA TCAGTGAACA AAAAAGACCA ATCGATGTAG AGTTTCAGAA AGGAGATCAG
    GGACTTCCTG GTGACCGAGG GCCTCCTGGA CCCCCAGGGA TACGTGGTCC TCCAGGTCCT
    CCAGGTGGTG TGAAAGGTGA GAAGGGTGAG CAAGGAGAGC CTGGCAAAAG AGGTAAACCA
    GGCAAAGATG GAGAGAATGG CCAACCAGGA ATTCCAGGTT TGCCTGGTGA TCCTGGTTAC
    CCTGGTGAAC CAGGAAGAGA TGGAGAAAAG GGTCAAAAAG GTGACACTGG CCTACCTGGG
    CCTCCTGGAC TTGTAATACC TAGGCCTGGG ACTGGTGTAA CTGTAGGAGA AAAAGGAAAC
    ATTGGGTTAC CTGGCTTGCC TGGAGATAAA GGAGATCGAG GATTTCCTGG AATACAAGGT
    CCACCCGGCC TTCCTGGACC TCCAGGACCT GCAGTTATAG GTCCTCCTGG ACCCCCTGGC
    TTTCCTGGGG AAAGGGGCCA GAAAGGTGAT GAAGGCCCAC CTGGAATTTC CATTCCTGGA
    TCTCCTGGAC TTGATGGACA GCCTGGGGCT CCTGGTCCTC CTGGCCCCCC TGGCCCTCCC
    GGCCCTCACA TCCCTCCTAG TGATAAGATA TGTGAAGCAG GCCCTCCTGG CCCTCCAGGA
    TCTCCAGGTG ATAGAGGACT CCAAGGAGAA CAAGGAATGA AAGGTGACAA AGGTGACACC
    TGCTTCAACT GTATTGGAAC TGGTGTTTCA GGGCCTCCAG GTCAGCCTGG TTTGCCAGGT
    CTTCCAGGTC CCCCAGGATC TCTTGGTTTC CCTGGACAGA AGGGCGAAAA AGGACATGCT
    GGTGCAACTG GTCCCAAAGG ATTATCAGGC ATACCAGGAG CTCCAGGTGC TCCAGGCTTT
    CCTGGACCTA AAGGTGAACC CGGTGACATC CTCACATTTC CAGGAATGAA GGGTGACAAA
    GGAGATTTGG GTTCCCCTGG AGCTCCAGGG CTTCCTGGTT TACCTGGCAC CCCTGGACAG
    GATGGATTGC CAGGACTTCC TGGCCCCAAA GGAGAACCTG GTGGAATGGC TTTTAAGGGT
    GAAAGAGGTC CCCCTGGGAA CCCAGGTTTG CCAGGTCTCC CAGGAAATAG GGGGCCTATG
    GGCCCTGTTG GTTTTGGTCC TCCTGGTCCA GTAGGTGAAA AAGGCGTACA AGGTGGGGCA
    GGAAATCCAG GCCAACCAGG AATACCAGGT CCTAAAGGTG ATCCAGGTCA GACCATAACC
    CAACCAGGGA AGCCTGGCCT GCCTGGTCAC CCAGGCAGAG ACGGTGAAGT AGGTCTTCCA
    GGTGAACCTG GACTCCCAGG CCAGCCAGGC TTGCCAGGAA TACCTGGTAG CAAAGGAGAA
    CCGGGTATCC CTGGAATCGG GCTTCCTGGA CCACCTGGTC CCAAAGGTTT TCCTGGAATT
    CCAGGACCTC CAGGAGCACC TGGATCACCT GGAAGAATCG GTCTAGAAGG TCCTTCTGGG
    CCACCAGGCT TTCCAGGACC AAAGGGAGAA CCAGGATTTG GGCTACCTGG GCCACCTGGG
    CCTCCAGGAC TCCCAGGTTT CAAAGGAATA CTTGGTCCAA AAGGGGATCG TGGTTTCCCA
    GGACCTCAAG GTCCTCCAGG ACAAGCTGGC TTGGATGGGC TACCTGGACC AAAAGGTGAC
    ATTGGACCAA ATGGACAACC TGGGCCAATT GGACCTCCTG GGCTGCCAGG AACAGGTTTT
    CAGGGACCAC CAGGGCCACC AGGAATTCCT GGACCAATAG GCCAACCTGG CTTGCATGGA
    CTACCAGGAG AGAAGGGGGA TCCAGGGCCT CCTGGATTTG ATGTTTCAGG ACCTCCTGGT
    GAGAGAGGCA GCCCAGGGAT CCCTGGAGCA CCAGGTCCTA TGGGACCCCC AGGATCACCA
    GGGCTCCCAG GAAAAGCAGG TGCCTCTGGC TTTCCAGGTG CCAAAGGTGA AATCGGTATG
    ATGGGACCTC CAGGCCCACC AGGACCTTTG GGAATTCCTG GCAGGAGTGG TGTTCCTGGT
    CTCAAAGGTG ATGATGGTTT GCAGGGTCAG CCAGGACTTC CTGGCCCTGC AGGAGAGAAA
    GGTAGTAAAG GAGAGCCTGG CCTTCCAGGC CTTCCTGGGC CAATGGATCC AGATCTGCTG
    GGCTCAAAAG GAGAGAAAGG GGACCCTGGA CTACCAGGTA TTCCTGGAGT TGCAGGGCCA
    AAAGGTTACC AGGGTTTGCC TGGAGACCCA GGGCAACCTG GACTGAGTGG ACAACCTGGA
    TTGCCAGGAC TAGCAGGTCC CAAGGGTAAC CCTGGTCTCC CTGGAATGCC AGGACTTACA
    GGACCTCCTG GACTGAAAGG AAACATGGGT GATATGGGTT TTCCAGGCCC ACAGGGTCCA
    AAAGGGTCTT TCGGACCTCC TGGAGTTCCT GGACAACCTG GCTCCCCAGG ACTACCTGGA
    CAGAAAGGAG AAAAAGGTGA TCCTGGTATT TCAGGCATTG GTCTTCCAGG TCTTCCTGGC
    GCAAAGGGTG AACCTGGTCT GCCTGGATAC CCAGGGAACC CTGGTACCAA AGGTTCTATG
    GGAGAAACTG GTTTGCCTGG ATTACCGGGG ACCCCTGGAG CAAAAGGACA ACCAGGCCTT
    CCTGGATTCC CTGGAACCCC AGGCCTTCCT GGACCAAAAG GTATTAGTGG TCCTCCTGGG
    AACCCTGGCC TTCCAGGAGA ACCTGGTCCT GTAGGTGGTG GAGGTCGTCC TGGGCCACCA
    GGGCCTCCAG GTGAAAAAGG CAAGCCAGGT CAAGATGGTA TTCCTGGACC AGCTGGACAG
    AAGGGTGAAC CAGGTCAACC AGGCTTTGGA ATCCCAGGAC CCCGTGGACT TCCAGGACTT
    TCTGGTCAAA AGGGTGATGA AGGATTACCT GGCATTCCAG GAAACCCTGG CCTTCCAGGT
    CCAAAGGGTG AACCAGGCTT TCATGGTTTC CCTGGTCTGC AGGGTCCCCC AGGCCCTCCT
    GGTTCTCCAG GTCCAGCTCT GGAAGGCCCT AAAGGCAGCC CTGGACCCCA AGGTCCTCCT
    GGGAGACCAG GTCCACCAGG TCCAGAAGGT CCCCGGGGTC TACCTGGAAG TGGGGCTATT
    AAAGGAGAAA GAGGTAACCC AGGCCAACCT GGGCAACCTG GTTTGCCTGG TTTGAAAGGT
    GATCAAGGTC CTCCAGGACT CCAGGGTAAC CCTGGCCGGC CAGGTCTCAA TGGAATGAAA
    GGAGATCCTG GTCTCCCTGG TGTTCCAGGA TTTCCAGGCA TGAAAGGACC CAGTGGAGTA
    CCTGGTTCAA CTGGCCCTGA GGGGGATCCA GGACTTATTG GCCCACCAGG TCCCCCTGGA
    TTACCTGGTC CTTCAGGACA GAGTATTGTA ATCAAAGGAG ATGCTGGCCC ACCAGGGATT
    CCAGGCCAGC CTGGATTAAA AGGTTTACAA GGACTCCCAG GACCTCAGGG TTTACCAGGT
    CCAATTGGCC CTCCAGGAGA TCCTGGACGC AATGGACTCC CTGGCTTTGA TGGTGCAGGA
    GGACGCAAAG GAGACCCAGG TTTGCCAGGC CAGCCAGGTA CTCGTGGCTT GGATGGTCCC
    CCCGGGCCAG ATGGATTGCA GGGTCCTCCA GGTCCCCCTG GAACCTCTTC TATTGCCCAT
    GGGTTTCTCA TCACACGTCA CAGCCAGACA ACAGATGCTC CACAATGCCC ACAGGGAACC
    ATCCAGGTGT ATGAAGGCTT TTCTCTCCTG TATGTACAAG GAAATAAAAG AGCTCATGGT
    CAAGACTTGG GAACAGCCGG CAGCTGCCTT CGTCGTTTCA GTACCATGCC TTTCATGTTC
    TGCAACATCA ATAATGTTTG CAACTTTGCT TCAAGAAATG ATTATTCTTA CTGGCTCTCC
    ACCCCAGAGC CCATGCCAAT GAGCATGGAA CCCCTGAAGG GGCAGAGCAT CCAGCCATTC
    ATTAGTCGAT GTGCAGTATG TGAAGCTCCG GCCGTGGTGA TTGCAGTCCA CAGTCAGACC
    ATCCAGATTC CCCGTTGTCC TCAGGGATGG GATTCTCTCT GGATTGGTTA TTCCTTCATG
    ATGCACACAA GTGCAGGGGC AGAGGGCTCA GGCCAAGCCC TGGCCTCCCC TGGTTCCTGC
    TTAGAAGAGT TTCGTTCAGC TCCCTTCATT GAGTGTCATG GGCGGGGGAC CTGCAACTAT
    TACGCCAACT CCTACAGCTT TTGGCTGGCG ACTGTAGATG TGTCAGACAT GTTCAGCAAA
    CCTCAATCAG AAACGCTGAA AGCAGGAGAC TTGAGGACAC GTATTAGCCG ATGTCAAGTG
    TGCATGAAGA GGACATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.