PARP8 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	PARP8
Entrez Gene ID	511595
Full Name	poly(ADP-ribose) polymerase family member 8
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005221534.3	XP_005221591.1	poly [ADP-ribose] polymerase 8 isoform X1
XM_005221535.3	XP_005221592.1	poly [ADP-ribose] polymerase 8 isoform X2
NM_001192298.1	NP_001179227.1	poly [ADP-ribose] polymerase 8
XM_005221535.4	XP_005221592.1	poly [ADP-ribose] polymerase 8 isoform X2
XM_005221534.4	XP_005221591.1	poly [ADP-ribose] polymerase 8 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PARP8	XP_853766.2
Bos taurus (cattle)	PARP8	NP_001179227.1
Danio rerio (zebrafish)	parp8	NP_001070643.1
Xenopus tropicalis (tropical clawed frog)	parp8	XP_002933075.1
Rattus norvegicus (Norway rat)	Parp8	XP_006232017.1
Mus musculus (house mouse)	Parp8	NP_001074478.1
Homo sapiens (human)	PARP8	NP_001171526.1
Pan troglodytes (chimpanzee)	PARP8	XP_001151176.2
	PARP8	XP_001093835.2
Gallus gallus (chicken)	PARP8	XP_424786.3

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PARP8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PARP8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39078	NM_001192298.1 Latest version!	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa30628	XM_005221534.4 Latest version!	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa30629	XM_005221535.4 Latest version!	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
View Old Accession Versions >>
OBa30629	XM_005221535.3	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
OBa30628	XM_005221534.3	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$510.30 $729.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PARP8 ( NM_001192298.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179227.1 Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), mRNA.

Price & Availability↑

CloneID	OBa39078
Clone ID Related Accession (Same CDS sequence)	NM_001192298.1
Accession Version	NM_001192298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000020.1. On Jul 17, 2010 this sequence version replaced XM_588962.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571260.48924.1, SRR5571257.52177.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGGGATGT GTTCAAGGCA AGAGCGAATT CAGAAGGATA TCGACGTCGT GATCCAGAAG 
TCCAGAGCCG AGAAGGACTG CCTGTTTGCA GATTTCAGAT ACTCAGACTC CACCTTTACT 
TTCACCTACG TTGGCGGCCC CAAAAGTGTA TCCTATTCAG TACATGTATC TGAAGATTAC 
CCAGATAATA CATATGTGTC AAGTTCAGAA AATGATGAAG ATGTATTAGT TACTACAGAG 
CCAATTCCAG TAATTTTTCA TCGACTAGCA ACAGAATTAA GAAAGACAAA TGACATTAAC 
TGTTGCTTAT CCATAAAATC CAAATTACAG AAGGAAAATG GCGAGGAGTC AAGACAGAAT 
AGCACAGTGG AGGAAGACTC TGAAGGAGAC AATGATTCGG AAGAATTTTA TTACGGAGGA 
CAGGTTAACT ATGACGGGGA GCTGCACAAA CACCCCCAGC TTGAAGCTGA CTTGTTGGCA 
GTGAGAGAGA TATATGGGCC ACATGCAGTT TCTCTGAGGG AATATGGAGC CATTGATGAT 
GTAGATATTG ATCTGCATAT TGATGTTAGC TTTCTTGATG AGGAGATTGC TGTGGCATGG 
GAAGTGATTC GAACAGAACC TATAATTGTC CGATTACACT GTTCTCTTAC ACAGTATTTG 
AATGGCCCAG TGCCCACTGT TGATGTCTTT CAGATTTCCA CAAAAGAGAG ATTTGGTCTG 
GGACATCAGC TGAAAAAAAT CATGCAGACA TTTGTTACAC AACAGTGGAA GCAGAGCAAA 
GAAAAATCCA ATTGCCTGCA CAATAAGAAG CTGTCAGAGA AGAAAGTCAA GTCTCCCCTG 
CATTTATTTT CAACCTTGCG CAGGTCGCCA AGTTATCCTC CCCCTGGTTG TGGAAAAAGC 
AAATCTAAAC TGAAATCGGA GCAGGACGGG ATCTCCAAGA CGCACAAGCT ACTGCGGAGG 
ACTTGTTCCA GCACCGTCAA GACGGAGGAC GTGTGCGCCC CGAAGCCACA CAGGACCTTC 
GGTCGCTCGC TGTCCAGCGA CCCCCGGGCT GAGCAGGCAG TGACAACCAT TAAGTCGCAC 
AAACTCTTGA ACCGTCCCTG CTCTGCAGCC GTCAAGCAGG AGGACTGCCT TGCTCTCAAG 
TCGCACAAAC TCTTGACCCG CTCTTGTTCC GGAGACCCGC GCTGTGAGCA CAATGCCAGC 
CTGAAGCCTC ACAAACTGTT AAGCAGGTCT TACTCCAGTA ACCTCAGAAT GGAAGAATTA 
TACGGACTCA AGAATCACAA GTTGCTCAGC AAGTCTTACT CCAGTGCCCC CAAGTCATCC 
AAAACGGAGC TTTTCAAGGA ACCCAACACA GAGGGCAGGA GGCTCTCTCT TACCTCAGGG 
CTCATTGGTA TCTTAACACC ATCGTCATCT TCATCTTCCC AGCCTGCTCC AAATGGTGCC 
AAATGCATTC CAATACGAGA CCGTGGCTTC CTAGTGCAGA CAATTGAGTT TGCTGAACAG 
CGGATCCCTG TGTTGAATGA GTACTGTGTG GTTTGTGATG AACCACATGT ATTTCAGAAT 
GGCCCCATGC TTAGGCCTAC GGTGTGTGAA CGAGAGCTGT GTGTATTTGC TTTTCAAACC 
CTGGGAGTAA TGAATGAAGC TGCTGATGAA ATCGCAACTG GAGCTCAGGT GGTAGATCTA 
CTAGTATCCA TGTGTAGGTC TGCATTGGAA TCTCCTAGAA AAGTTGTCAT TTTCGAGCCA 
TATCCTTCTG TGGTAGATCC TAACGATCCT CAGATGCTGG CCTTCAACCC CAGGAAGAAG 
AACTATGATC GAGTAATGAA AGCATTGGAT AGCATAACTT CTATTAGAGA AATGACACAA 
GCACCATATC TGGAAATCAA GAAGCAGATG GATAAACAAG ACCCCCTTGC TCATCCCTTA 
CTGCAATGGG TTATTTCAAG TAATAGGTCA CATATTGTGA AACTGCCAGT TAACAGGCAA 
CTGAAGTTCA TGCACACACC CCATCAGTTC CTTCTTCTCA GCAGTCCACC AGCCAAAGAA 
TCCAATTTTA GAGCTGCTAA AAAACTCTTC GGAAGCACCT TTGCATTTCA TGGCTCACAC 
ATTGAAAACT GGCATTCCAT CCTGAGGAAT GGTCTGGTTG TTGCTTCTAA TACACGACTG 
CAGCTCCACG GTGCAATGTA TGGAAGTGGA ATCTATCTTA GTCCAATGTC AAGCATATCA 
TTTGGTTACT CAGGGATGAA CAAGAAGCAG AAGGTGTCAG CCAAGGATGA GCCGGCTTCA 
AGCAGTAAAA ACAGCAACGC ATCACAGTCA CAGAAGAAAG GACAGCAATC CCAGTTCCTG 
CAAAGCCGTA ACTTAAAATG CATAGCCTTA TGTGAAGTGA TCACCTCACC TGACCTGCAC 
AAACATGGAG AGATATGGGT CGTCCCAAAT ACTGATCATG TCTGCACAAG ATTCTTTTTT 
GTCTATGAAG ATGGCCAAGT GGGAGACGCA AATATTAACA CGCAAGAAGG AGGCATTCAC 
AAAGAGATCC TTCGAGTAAT TGGTAATCAA ACTGCTACTG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179227.1
CDS	1..2565
Translation	MGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRLATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLLAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTEDVCAPKPHRTFGRSLSSDPRAEQAVTTIKSHKLLNRPCSAAVKQEDCLALKSHKLLTRSCSGDPRCEHNASLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNTEGRRLSLTSGLIGILTPSSSSSSQPAPNGAKCIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKNSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG

Target ORF information:

RefSeq Version	NM_001192298.1
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192298.1

ORF Insert Sequence:

ATGGGGATGT GTTCAAGGCA AGAGCGAATT CAGAAGGATA TCGACGTCGT GATCCAGAAG 
TCCAGAGCCG AGAAGGACTG CCTGTTTGCA GATTTCAGAT ACTCAGACTC CACCTTTACT 
TTCACCTACG TTGGCGGCCC CAAAAGTGTA TCCTATTCAG TACATGTATC TGAAGATTAC 
CCAGATAATA CATATGTGTC AAGTTCAGAA AATGATGAAG ATGTATTAGT TACTACAGAG 
CCAATTCCAG TAATTTTTCA TCGACTAGCA ACAGAATTAA GAAAGACAAA TGACATTAAC 
TGTTGCTTAT CCATAAAATC CAAATTACAG AAGGAAAATG GCGAGGAGTC AAGACAGAAT 
AGCACAGTGG AGGAAGACTC TGAAGGAGAC AATGATTCGG AAGAATTTTA TTACGGAGGA 
CAGGTTAACT ATGACGGGGA GCTGCACAAA CACCCCCAGC TTGAAGCTGA CTTGTTGGCA 
GTGAGAGAGA TATATGGGCC ACATGCAGTT TCTCTGAGGG AATATGGAGC CATTGATGAT 
GTAGATATTG ATCTGCATAT TGATGTTAGC TTTCTTGATG AGGAGATTGC TGTGGCATGG 
GAAGTGATTC GAACAGAACC TATAATTGTC CGATTACACT GTTCTCTTAC ACAGTATTTG 
AATGGCCCAG TGCCCACTGT TGATGTCTTT CAGATTTCCA CAAAAGAGAG ATTTGGTCTG 
GGACATCAGC TGAAAAAAAT CATGCAGACA TTTGTTACAC AACAGTGGAA GCAGAGCAAA 
GAAAAATCCA ATTGCCTGCA CAATAAGAAG CTGTCAGAGA AGAAAGTCAA GTCTCCCCTG 
CATTTATTTT CAACCTTGCG CAGGTCGCCA AGTTATCCTC CCCCTGGTTG TGGAAAAAGC 
AAATCTAAAC TGAAATCGGA GCAGGACGGG ATCTCCAAGA CGCACAAGCT ACTGCGGAGG 
ACTTGTTCCA GCACCGTCAA GACGGAGGAC GTGTGCGCCC CGAAGCCACA CAGGACCTTC 
GGTCGCTCGC TGTCCAGCGA CCCCCGGGCT GAGCAGGCAG TGACAACCAT TAAGTCGCAC 
AAACTCTTGA ACCGTCCCTG CTCTGCAGCC GTCAAGCAGG AGGACTGCCT TGCTCTCAAG 
TCGCACAAAC TCTTGACCCG CTCTTGTTCC GGAGACCCGC GCTGTGAGCA CAATGCCAGC 
CTGAAGCCTC ACAAACTGTT AAGCAGGTCT TACTCCAGTA ACCTCAGAAT GGAAGAATTA 
TACGGACTCA AGAATCACAA GTTGCTCAGC AAGTCTTACT CCAGTGCCCC CAAGTCATCC 
AAAACGGAGC TTTTCAAGGA ACCCAACACA GAGGGCAGGA GGCTCTCTCT TACCTCAGGG 
CTCATTGGTA TCTTAACACC ATCGTCATCT TCATCTTCCC AGCCTGCTCC AAATGGTGCC 
AAATGCATTC CAATACGAGA CCGTGGCTTC CTAGTGCAGA CAATTGAGTT TGCTGAACAG 
CGGATCCCTG TGTTGAATGA GTACTGTGTG GTTTGTGATG AACCACATGT ATTTCAGAAT 
GGCCCCATGC TTAGGCCTAC GGTGTGTGAA CGAGAGCTGT GTGTATTTGC TTTTCAAACC 
CTGGGAGTAA TGAATGAAGC TGCTGATGAA ATCGCAACTG GAGCTCAGGT GGTAGATCTA 
CTAGTATCCA TGTGTAGGTC TGCATTGGAA TCTCCTAGAA AAGTTGTCAT TTTCGAGCCA 
TATCCTTCTG TGGTAGATCC TAACGATCCT CAGATGCTGG CCTTCAACCC CAGGAAGAAG 
AACTATGATC GAGTAATGAA AGCATTGGAT AGCATAACTT CTATTAGAGA AATGACACAA 
GCACCATATC TGGAAATCAA GAAGCAGATG GATAAACAAG ACCCCCTTGC TCATCCCTTA 
CTGCAATGGG TTATTTCAAG TAATAGGTCA CATATTGTGA AACTGCCAGT TAACAGGCAA 
CTGAAGTTCA TGCACACACC CCATCAGTTC CTTCTTCTCA GCAGTCCACC AGCCAAAGAA 
TCCAATTTTA GAGCTGCTAA AAAACTCTTC GGAAGCACCT TTGCATTTCA TGGCTCACAC 
ATTGAAAACT GGCATTCCAT CCTGAGGAAT GGTCTGGTTG TTGCTTCTAA TACACGACTG 
CAGCTCCACG GTGCAATGTA TGGAAGTGGA ATCTATCTTA GTCCAATGTC AAGCATATCA 
TTTGGTTACT CAGGGATGAA CAAGAAGCAG AAGGTGTCAG CCAAGGATGA GCCGGCTTCA 
AGCAGTAAAA ACAGCAACGC ATCACAGTCA CAGAAGAAAG GACAGCAATC CCAGTTCCTG 
CAAAGCCGTA ACTTAAAATG CATAGCCTTA TGTGAAGTGA TCACCTCACC TGACCTGCAC 
AAACATGGAG AGATATGGGT CGTCCCAAAT ACTGATCATG TCTGCACAAG ATTCTTTTTT 
GTCTATGAAG ATGGCCAAGT GGGAGACGCA AATATTAACA CGCAAGAAGG AGGCATTCAC 
AAAGAGATCC TTCGAGTAAT TGGTAATCAA ACTGCTACTG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP8 ( XM_005221534.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221591.1 Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa30628
Clone ID Related Accession (Same CDS sequence)	XM_005221534.3 , XM_005221534.4
Accession Version	XM_005221534.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104513.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGCC GACCTCCCCC GCCTCCTCCT CCGCCACCAC CGCCTCCTCT CTCACCCGGG 
ATCACTTCCG AAACCACTTC GCCTTCAGCC CCTGCCTCGG CCAGAGGGAT TTATTTAATG 
GGGATGTGTT CAAGGCAAGA GCGAATTCAG AAGGATATCG ACGTCGTGAT CCAGAAGTCC 
AGAGCCGAGA AGGACTGCCT GTTTGCAGAT TTCAGATACT CAGACTCCAC CTTTACTTTC 
ACCTACGTTG GCGGCCCCAA AAGTGTATCC TATTCAGTAC ATGTATCTGA AGATTACCCA 
GATAATACAT ATGTGTCAAG TTCAGAAAAT GATGAAGATG TATTAGTTAC TACAGAGCCA 
ATTCCAGTAA TTTTTCATCG ACTAGCAACA GAATTAAGAA AGACAAATGA CATTAACTGT 
TGCTTATCCA TAAAATCCAA ATTACAGAAG GAAAATGGCG AGGAGTCAAG ACAGAATAGC 
ACAGTGGAGG AAGACTCTGA AGGAGACAAT GATTCGGAAG AATTTTATTA CGGAGGACAG 
GTTAACTATG ACGGGGAGCT GCACAAACAC CCCCAGCTTG AAGCTGACTT GTTGGCAGTG 
AGAGAGATAT ATGGGCCACA TGCAGTTTCT CTGAGGGAAT ATGGAGCCAT TGATGATGTA 
GATATTGATC TGCATATTGA TGTTAGCTTT CTTGATGAGG AGATTGCTGT GGCATGGGAA 
GTGATTCGAA CAGAACCTAT AATTGTCCGA TTACACTGTT CTCTTACACA GTATTTGAAT 
GGCCCAGTGC CCACTGTTGA TGTCTTTCAG ATTTCCACAA AAGAGAGATT TGGTCTGGGA 
CATCAGCTGA AAAAAATCAT GCAGACATTT GTTACACAAC AGTGGAAGCA GAGCAAAGAA 
AAATCCAATT GCCTGCACAA TAAGAAGCTG TCAGAGAAGA AAGTCAAGTC TCCCCTGCAT 
TTATTTTCAA CCTTGCGCAG GTCGCCAAGT TATCCTCCCC CTGGTTGTGG AAAAAGCAAA 
TCTAAACTGA AATCGGAGCA GGACGGGATC TCCAAGACGC ACAAGCTACT GCGGAGGACT 
TGTTCCAGCA CCGTCAAGAC GGAGGACGTG TGCGCCCCGA AGCCACACAG GACCTTCGGT 
CGCTCGCTGT CCAGCGACCC CCGGGCTGAG CAGGCAGTGA CAACCATTAA GTCGCACAAA 
CTCTTGAACC GTCCCTGCTC TGCAGCCGTC AAGCAGGAGG ACTGCCTTGC TCTCAAGTCG 
CACAAACTCT TGACCCGCTC TTGTTCCGGA GACCCGCGCT GTGAGCACAA TGCCAGCCTG 
AAGCCTCACA AACTGTTAAG CAGGTCTTAC TCCAGTAACC TCAGAATGGA AGAATTATAC 
GGACTCAAGA ATCACAAGTT GCTCAGCAAG TCTTACTCCA GTGCCCCCAA GTCATCCAAA 
ACGGAGCTTT TCAAGGAACC CAACACAGAG GGCAGGAGGC TCTCTCTTAC CTCAGGGCTC 
ATTGGTATCT TAACACCATC GTCATCTTCA TCTTCCCAGC CTGCTCCAAA TGGTGCCAAA 
TGCATTCCAA TACGAGACCG TGGCTTCCTA GTGCAGACAA TTGAGTTTGC TGAACAGCGG 
ATCCCTGTGT TGAATGAGTA CTGTGTGGTT TGTGATGAAC CACATGTATT TCAGAATGGC 
CCCATGCTTA GGCCTACGGT GTGTGAACGA GAGCTGTGTG TATTTGCTTT TCAAACCCTG 
GGAGTAATGA ATGAAGCTGC TGATGAAATC GCAACTGGAG CTCAGGTGGT AGATCTACTA 
GTATCCATGT GTAGGTCTGC ATTGGAATCT CCTAGAAAAG TTGTCATTTT CGAGCCATAT 
CCTTCTGTGG TAGATCCTAA CGATCCTCAG ATGCTGGCCT TCAACCCCAG GAAGAAGAAC 
TATGATCGAG TAATGAAAGC ATTGGATAGC ATAACTTCTA TTAGAGAAAT GACACAAGCA 
CCATATCTGG AAATCAAGAA GCAGATGGAT AAACAAGACC CCCTTGCTCA TCCCTTACTG 
CAATGGGTTA TTTCAAGTAA TAGGTCACAT ATTGTGAAAC TGCCAGTTAA CAGGCAACTG 
AAGTTCATGC ACACACCCCA TCAGTTCCTT CTTCTCAGCA GTCCACCAGC CAAAGAATCC 
AATTTTAGAG CTGCTAAAAA ACTCTTCGGA AGCACCTTTG CATTTCATGG CTCACACATT 
GAAAACTGGC ATTCCATCCT GAGGAATGGT CTGGTTGTTG CTTCTAATAC ACGACTGCAG 
CTCCACGGTG CAATGTATGG AAGTGGAATC TATCTTAGTC CAATGTCAAG CATATCATTT 
GGTTACTCAG GGATGAACAA GAAGCAGAAG GTGTCAGCCA AGGATGAGCC GGCTTCAAGC 
AGTAAAAACA GCAACGCATC ACAGTCACAG AAGAAAGGAC AGCAATCCCA GTTCCTGCAA 
AGCCGTAACT TAAAATGCAT AGCCTTATGT GAAGTGATCA CCTCACCTGA CCTGCACAAA 
CATGGAGAGA TATGGGTCGT CCCAAATACT GATCATGTCT GCACAAGATT CTTTTTTGTC 
TATGAAGATG GCCAAGTGGG AGACGCAAAT ATTAACACGC AAGAAGGAGG CATTCACAAA 
GAGATCCTTC GAGTAATTGG TAATCAAACT GCTACTGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221591.1
CDS	211..2892
Translation	MQSRPPPPPPPPPPPPLSPGITSETTSPSAPASARGIYLMGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRLATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLLAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTEDVCAPKPHRTFGRSLSSDPRAEQAVTTIKSHKLLNRPCSAAVKQEDCLALKSHKLLTRSCSGDPRCEHNASLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNTEGRRLSLTSGLIGILTPSSSSSSQPAPNGAKCIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKNSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG

Target ORF information:

RefSeq Version	XM_005221534.3
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221534.3

ORF Insert Sequence:

ATGCAAAGCC GACCTCCCCC GCCTCCTCCT CCGCCACCAC CGCCTCCTCT CTCACCCGGG 
ATCACTTCCG AAACCACTTC GCCTTCAGCC CCTGCCTCGG CCAGAGGGAT TTATTTAATG 
GGGATGTGTT CAAGGCAAGA GCGAATTCAG AAGGATATCG ACGTCGTGAT CCAGAAGTCC 
AGAGCCGAGA AGGACTGCCT GTTTGCAGAT TTCAGATACT CAGACTCCAC CTTTACTTTC 
ACCTACGTTG GCGGCCCCAA AAGTGTATCC TATTCAGTAC ATGTATCTGA AGATTACCCA 
GATAATACAT ATGTGTCAAG TTCAGAAAAT GATGAAGATG TATTAGTTAC TACAGAGCCA 
ATTCCAGTAA TTTTTCATCG ACTAGCAACA GAATTAAGAA AGACAAATGA CATTAACTGT 
TGCTTATCCA TAAAATCCAA ATTACAGAAG GAAAATGGCG AGGAGTCAAG ACAGAATAGC 
ACAGTGGAGG AAGACTCTGA AGGAGACAAT GATTCGGAAG AATTTTATTA CGGAGGACAG 
GTTAACTATG ACGGGGAGCT GCACAAACAC CCCCAGCTTG AAGCTGACTT GTTGGCAGTG 
AGAGAGATAT ATGGGCCACA TGCAGTTTCT CTGAGGGAAT ATGGAGCCAT TGATGATGTA 
GATATTGATC TGCATATTGA TGTTAGCTTT CTTGATGAGG AGATTGCTGT GGCATGGGAA 
GTGATTCGAA CAGAACCTAT AATTGTCCGA TTACACTGTT CTCTTACACA GTATTTGAAT 
GGCCCAGTGC CCACTGTTGA TGTCTTTCAG ATTTCCACAA AAGAGAGATT TGGTCTGGGA 
CATCAGCTGA AAAAAATCAT GCAGACATTT GTTACACAAC AGTGGAAGCA GAGCAAAGAA 
AAATCCAATT GCCTGCACAA TAAGAAGCTG TCAGAGAAGA AAGTCAAGTC TCCCCTGCAT 
TTATTTTCAA CCTTGCGCAG GTCGCCAAGT TATCCTCCCC CTGGTTGTGG AAAAAGCAAA 
TCTAAACTGA AATCGGAGCA GGACGGGATC TCCAAGACGC ACAAGCTACT GCGGAGGACT 
TGTTCCAGCA CCGTCAAGAC GGAGGACGTG TGCGCCCCGA AGCCACACAG GACCTTCGGT 
CGCTCGCTGT CCAGCGACCC CCGGGCTGAG CAGGCAGTGA CAACCATTAA GTCGCACAAA 
CTCTTGAACC GTCCCTGCTC TGCAGCCGTC AAGCAGGAGG ACTGCCTTGC TCTCAAGTCG 
CACAAACTCT TGACCCGCTC TTGTTCCGGA GACCCGCGCT GTGAGCACAA TGCCAGCCTG 
AAGCCTCACA AACTGTTAAG CAGGTCTTAC TCCAGTAACC TCAGAATGGA AGAATTATAC 
GGACTCAAGA ATCACAAGTT GCTCAGCAAG TCTTACTCCA GTGCCCCCAA GTCATCCAAA 
ACGGAGCTTT TCAAGGAACC CAACACAGAG GGCAGGAGGC TCTCTCTTAC CTCAGGGCTC 
ATTGGTATCT TAACACCATC GTCATCTTCA TCTTCCCAGC CTGCTCCAAA TGGTGCCAAA 
TGCATTCCAA TACGAGACCG TGGCTTCCTA GTGCAGACAA TTGAGTTTGC TGAACAGCGG 
ATCCCTGTGT TGAATGAGTA CTGTGTGGTT TGTGATGAAC CACATGTATT TCAGAATGGC 
CCCATGCTTA GGCCTACGGT GTGTGAACGA GAGCTGTGTG TATTTGCTTT TCAAACCCTG 
GGAGTAATGA ATGAAGCTGC TGATGAAATC GCAACTGGAG CTCAGGTGGT AGATCTACTA 
GTATCCATGT GTAGGTCTGC ATTGGAATCT CCTAGAAAAG TTGTCATTTT CGAGCCATAT 
CCTTCTGTGG TAGATCCTAA CGATCCTCAG ATGCTGGCCT TCAACCCCAG GAAGAAGAAC 
TATGATCGAG TAATGAAAGC ATTGGATAGC ATAACTTCTA TTAGAGAAAT GACACAAGCA 
CCATATCTGG AAATCAAGAA GCAGATGGAT AAACAAGACC CCCTTGCTCA TCCCTTACTG 
CAATGGGTTA TTTCAAGTAA TAGGTCACAT ATTGTGAAAC TGCCAGTTAA CAGGCAACTG 
AAGTTCATGC ACACACCCCA TCAGTTCCTT CTTCTCAGCA GTCCACCAGC CAAAGAATCC 
AATTTTAGAG CTGCTAAAAA ACTCTTCGGA AGCACCTTTG CATTTCATGG CTCACACATT 
GAAAACTGGC ATTCCATCCT GAGGAATGGT CTGGTTGTTG CTTCTAATAC ACGACTGCAG 
CTCCACGGTG CAATGTATGG AAGTGGAATC TATCTTAGTC CAATGTCAAG CATATCATTT 
GGTTACTCAG GGATGAACAA GAAGCAGAAG GTGTCAGCCA AGGATGAGCC GGCTTCAAGC 
AGTAAAAACA GCAACGCATC ACAGTCACAG AAGAAAGGAC AGCAATCCCA GTTCCTGCAA 
AGCCGTAACT TAAAATGCAT AGCCTTATGT GAAGTGATCA CCTCACCTGA CCTGCACAAA 
CATGGAGAGA TATGGGTCGT CCCAAATACT GATCATGTCT GCACAAGATT CTTTTTTGTC 
TATGAAGATG GCCAAGTGGG AGACGCAAAT ATTAACACGC AAGAAGGAGG CATTCACAAA 
GAGATCCTTC GAGTAATTGG TAATCAAACT GCTACTGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP8 ( XM_005221534.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221591.1 Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.

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CloneID	OBa30628
Clone ID Related Accession (Same CDS sequence)	XM_005221534.3 , XM_005221534.4
Accession Version	XM_005221534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221534.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAGCC GACCTCCCCC GCCTCCTCCT CCGCCACCAC CGCCTCCTCT CTCACCCGGG 
ATCACTTCCG AAACCACTTC GCCTTCAGCC CCTGCCTCGG CCAGAGGGAT TTATTTAATG 
GGGATGTGTT CAAGGCAAGA GCGAATTCAG AAGGATATCG ACGTCGTGAT CCAGAAGTCC 
AGAGCCGAGA AGGACTGCCT GTTTGCAGAT TTCAGATACT CAGACTCCAC CTTTACTTTC 
ACCTACGTTG GCGGCCCCAA AAGTGTATCC TATTCAGTAC ATGTATCTGA AGATTACCCA 
GATAATACAT ATGTGTCAAG TTCAGAAAAT GATGAAGATG TATTAGTTAC TACAGAGCCA 
ATTCCAGTAA TTTTTCATCG ACTAGCAACA GAATTAAGAA AGACAAATGA CATTAACTGT 
TGCTTATCCA TAAAATCCAA ATTACAGAAG GAAAATGGCG AGGAGTCAAG ACAGAATAGC 
ACAGTGGAGG AAGACTCTGA AGGAGACAAT GATTCGGAAG AATTTTATTA CGGAGGACAG 
GTTAACTATG ACGGGGAGCT GCACAAACAC CCCCAGCTTG AAGCTGACTT GTTGGCAGTG 
AGAGAGATAT ATGGGCCACA TGCAGTTTCT CTGAGGGAAT ATGGAGCCAT TGATGATGTA 
GATATTGATC TGCATATTGA TGTTAGCTTT CTTGATGAGG AGATTGCTGT GGCATGGGAA 
GTGATTCGAA CAGAACCTAT AATTGTCCGA TTACACTGTT CTCTTACACA GTATTTGAAT 
GGCCCAGTGC CCACTGTTGA TGTCTTTCAG ATTTCCACAA AAGAGAGATT TGGTCTGGGA 
CATCAGCTGA AAAAAATCAT GCAGACATTT GTTACACAAC AGTGGAAGCA GAGCAAAGAA 
AAATCCAATT GCCTGCACAA TAAGAAGCTG TCAGAGAAGA AAGTCAAGTC TCCCCTGCAT 
TTATTTTCAA CCTTGCGCAG GTCGCCAAGT TATCCTCCCC CTGGTTGTGG AAAAAGCAAA 
TCTAAACTGA AATCGGAGCA GGACGGGATC TCCAAGACGC ACAAGCTACT GCGGAGGACT 
TGTTCCAGCA CCGTCAAGAC GGAGGACGTG TGCGCCCCGA AGCCACACAG GACCTTCGGT 
CGCTCGCTGT CCAGCGACCC CCGGGCTGAG CAGGCAGTGA CAACCATTAA GTCGCACAAA 
CTCTTGAACC GTCCCTGCTC TGCAGCCGTC AAGCAGGAGG ACTGCCTTGC TCTCAAGTCG 
CACAAACTCT TGACCCGCTC TTGTTCCGGA GACCCGCGCT GTGAGCACAA TGCCAGCCTG 
AAGCCTCACA AACTGTTAAG CAGGTCTTAC TCCAGTAACC TCAGAATGGA AGAATTATAC 
GGACTCAAGA ATCACAAGTT GCTCAGCAAG TCTTACTCCA GTGCCCCCAA GTCATCCAAA 
ACGGAGCTTT TCAAGGAACC CAACACAGAG GGCAGGAGGC TCTCTCTTAC CTCAGGGCTC 
ATTGGTATCT TAACACCATC GTCATCTTCA TCTTCCCAGC CTGCTCCAAA TGGTGCCAAA 
TGCATTCCAA TACGAGACCG TGGCTTCCTA GTGCAGACAA TTGAGTTTGC TGAACAGCGG 
ATCCCTGTGT TGAATGAGTA CTGTGTGGTT TGTGATGAAC CACATGTATT TCAGAATGGC 
CCCATGCTTA GGCCTACGGT GTGTGAACGA GAGCTGTGTG TATTTGCTTT TCAAACCCTG 
GGAGTAATGA ATGAAGCTGC TGATGAAATC GCAACTGGAG CTCAGGTGGT AGATCTACTA 
GTATCCATGT GTAGGTCTGC ATTGGAATCT CCTAGAAAAG TTGTCATTTT CGAGCCATAT 
CCTTCTGTGG TAGATCCTAA CGATCCTCAG ATGCTGGCCT TCAACCCCAG GAAGAAGAAC 
TATGATCGAG TAATGAAAGC ATTGGATAGC ATAACTTCTA TTAGAGAAAT GACACAAGCA 
CCATATCTGG AAATCAAGAA GCAGATGGAT AAACAAGACC CCCTTGCTCA TCCCTTACTG 
CAATGGGTTA TTTCAAGTAA TAGGTCACAT ATTGTGAAAC TGCCAGTTAA CAGGCAACTG 
AAGTTCATGC ACACACCCCA TCAGTTCCTT CTTCTCAGCA GTCCACCAGC CAAAGAATCC 
AATTTTAGAG CTGCTAAAAA ACTCTTCGGA AGCACCTTTG CATTTCATGG CTCACACATT 
GAAAACTGGC ATTCCATCCT GAGGAATGGT CTGGTTGTTG CTTCTAATAC ACGACTGCAG 
CTCCACGGTG CAATGTATGG AAGTGGAATC TATCTTAGTC CAATGTCAAG CATATCATTT 
GGTTACTCAG GGATGAACAA GAAGCAGAAG GTGTCAGCCA AGGATGAGCC GGCTTCAAGC 
AGTAAAAACA GCAACGCATC ACAGTCACAG AAGAAAGGAC AGCAATCCCA GTTCCTGCAA 
AGCCGTAACT TAAAATGCAT AGCCTTATGT GAAGTGATCA CCTCACCTGA CCTGCACAAA 
CATGGAGAGA TATGGGTCGT CCCAAATACT GATCATGTCT GCACAAGATT CTTTTTTGTC 
TATGAAGATG GCCAAGTGGG AGACGCAAAT ATTAACACGC AAGAAGGAGG CATTCACAAA 
GAGATCCTTC GAGTAATTGG TAATCAAACT GCTACTGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221591.1
CDS	252..2933
Translation	MQSRPPPPPPPPPPPPLSPGITSETTSPSAPASARGIYLMGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRLATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLLAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTEDVCAPKPHRTFGRSLSSDPRAEQAVTTIKSHKLLNRPCSAAVKQEDCLALKSHKLLTRSCSGDPRCEHNASLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNTEGRRLSLTSGLIGILTPSSSSSSQPAPNGAKCIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKNSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG

Target ORF information:

RefSeq Version	XM_005221534.4
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221534.4

ORF Insert Sequence:

ATGCAAAGCC GACCTCCCCC GCCTCCTCCT CCGCCACCAC CGCCTCCTCT CTCACCCGGG 
ATCACTTCCG AAACCACTTC GCCTTCAGCC CCTGCCTCGG CCAGAGGGAT TTATTTAATG 
GGGATGTGTT CAAGGCAAGA GCGAATTCAG AAGGATATCG ACGTCGTGAT CCAGAAGTCC 
AGAGCCGAGA AGGACTGCCT GTTTGCAGAT TTCAGATACT CAGACTCCAC CTTTACTTTC 
ACCTACGTTG GCGGCCCCAA AAGTGTATCC TATTCAGTAC ATGTATCTGA AGATTACCCA 
GATAATACAT ATGTGTCAAG TTCAGAAAAT GATGAAGATG TATTAGTTAC TACAGAGCCA 
ATTCCAGTAA TTTTTCATCG ACTAGCAACA GAATTAAGAA AGACAAATGA CATTAACTGT 
TGCTTATCCA TAAAATCCAA ATTACAGAAG GAAAATGGCG AGGAGTCAAG ACAGAATAGC 
ACAGTGGAGG AAGACTCTGA AGGAGACAAT GATTCGGAAG AATTTTATTA CGGAGGACAG 
GTTAACTATG ACGGGGAGCT GCACAAACAC CCCCAGCTTG AAGCTGACTT GTTGGCAGTG 
AGAGAGATAT ATGGGCCACA TGCAGTTTCT CTGAGGGAAT ATGGAGCCAT TGATGATGTA 
GATATTGATC TGCATATTGA TGTTAGCTTT CTTGATGAGG AGATTGCTGT GGCATGGGAA 
GTGATTCGAA CAGAACCTAT AATTGTCCGA TTACACTGTT CTCTTACACA GTATTTGAAT 
GGCCCAGTGC CCACTGTTGA TGTCTTTCAG ATTTCCACAA AAGAGAGATT TGGTCTGGGA 
CATCAGCTGA AAAAAATCAT GCAGACATTT GTTACACAAC AGTGGAAGCA GAGCAAAGAA 
AAATCCAATT GCCTGCACAA TAAGAAGCTG TCAGAGAAGA AAGTCAAGTC TCCCCTGCAT 
TTATTTTCAA CCTTGCGCAG GTCGCCAAGT TATCCTCCCC CTGGTTGTGG AAAAAGCAAA 
TCTAAACTGA AATCGGAGCA GGACGGGATC TCCAAGACGC ACAAGCTACT GCGGAGGACT 
TGTTCCAGCA CCGTCAAGAC GGAGGACGTG TGCGCCCCGA AGCCACACAG GACCTTCGGT 
CGCTCGCTGT CCAGCGACCC CCGGGCTGAG CAGGCAGTGA CAACCATTAA GTCGCACAAA 
CTCTTGAACC GTCCCTGCTC TGCAGCCGTC AAGCAGGAGG ACTGCCTTGC TCTCAAGTCG 
CACAAACTCT TGACCCGCTC TTGTTCCGGA GACCCGCGCT GTGAGCACAA TGCCAGCCTG 
AAGCCTCACA AACTGTTAAG CAGGTCTTAC TCCAGTAACC TCAGAATGGA AGAATTATAC 
GGACTCAAGA ATCACAAGTT GCTCAGCAAG TCTTACTCCA GTGCCCCCAA GTCATCCAAA 
ACGGAGCTTT TCAAGGAACC CAACACAGAG GGCAGGAGGC TCTCTCTTAC CTCAGGGCTC 
ATTGGTATCT TAACACCATC GTCATCTTCA TCTTCCCAGC CTGCTCCAAA TGGTGCCAAA 
TGCATTCCAA TACGAGACCG TGGCTTCCTA GTGCAGACAA TTGAGTTTGC TGAACAGCGG 
ATCCCTGTGT TGAATGAGTA CTGTGTGGTT TGTGATGAAC CACATGTATT TCAGAATGGC 
CCCATGCTTA GGCCTACGGT GTGTGAACGA GAGCTGTGTG TATTTGCTTT TCAAACCCTG 
GGAGTAATGA ATGAAGCTGC TGATGAAATC GCAACTGGAG CTCAGGTGGT AGATCTACTA 
GTATCCATGT GTAGGTCTGC ATTGGAATCT CCTAGAAAAG TTGTCATTTT CGAGCCATAT 
CCTTCTGTGG TAGATCCTAA CGATCCTCAG ATGCTGGCCT TCAACCCCAG GAAGAAGAAC 
TATGATCGAG TAATGAAAGC ATTGGATAGC ATAACTTCTA TTAGAGAAAT GACACAAGCA 
CCATATCTGG AAATCAAGAA GCAGATGGAT AAACAAGACC CCCTTGCTCA TCCCTTACTG 
CAATGGGTTA TTTCAAGTAA TAGGTCACAT ATTGTGAAAC TGCCAGTTAA CAGGCAACTG 
AAGTTCATGC ACACACCCCA TCAGTTCCTT CTTCTCAGCA GTCCACCAGC CAAAGAATCC 
AATTTTAGAG CTGCTAAAAA ACTCTTCGGA AGCACCTTTG CATTTCATGG CTCACACATT 
GAAAACTGGC ATTCCATCCT GAGGAATGGT CTGGTTGTTG CTTCTAATAC ACGACTGCAG 
CTCCACGGTG CAATGTATGG AAGTGGAATC TATCTTAGTC CAATGTCAAG CATATCATTT 
GGTTACTCAG GGATGAACAA GAAGCAGAAG GTGTCAGCCA AGGATGAGCC GGCTTCAAGC 
AGTAAAAACA GCAACGCATC ACAGTCACAG AAGAAAGGAC AGCAATCCCA GTTCCTGCAA 
AGCCGTAACT TAAAATGCAT AGCCTTATGT GAAGTGATCA CCTCACCTGA CCTGCACAAA 
CATGGAGAGA TATGGGTCGT CCCAAATACT GATCATGTCT GCACAAGATT CTTTTTTGTC 
TATGAAGATG GCCAAGTGGG AGACGCAAAT ATTAACACGC AAGAAGGAGG CATTCACAAA 
GAGATCCTTC GAGTAATTGG TAATCAAACT GCTACTGGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP8 ( XM_005221535.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221592.1 Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa30629
Clone ID Related Accession (Same CDS sequence)	XM_005221535.3 , XM_005221535.4
Accession Version	XM_005221535.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104513.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAA AGACCGAGCA GAGCAGAACG GGGATTTATT TAATGGGGAT GTGTTCAAGG 
CAAGAGCGAA TTCAGAAGGA TATCGACGTC GTGATCCAGA AGTCCAGAGC CGAGAAGGAC 
TGCCTGTTTG CAGATTTCAG ATACTCAGAC TCCACCTTTA CTTTCACCTA CGTTGGCGGC 
CCCAAAAGTG TATCCTATTC AGTACATGTA TCTGAAGATT ACCCAGATAA TACATATGTG 
TCAAGTTCAG AAAATGATGA AGATGTATTA GTTACTACAG AGCCAATTCC AGTAATTTTT 
CATCGACTAG CAACAGAATT AAGAAAGACA AATGACATTA ACTGTTGCTT ATCCATAAAA 
TCCAAATTAC AGAAGGAAAA TGGCGAGGAG TCAAGACAGA ATAGCACAGT GGAGGAAGAC 
TCTGAAGGAG ACAATGATTC GGAAGAATTT TATTACGGAG GACAGGTTAA CTATGACGGG 
GAGCTGCACA AACACCCCCA GCTTGAAGCT GACTTGTTGG CAGTGAGAGA GATATATGGG 
CCACATGCAG TTTCTCTGAG GGAATATGGA GCCATTGATG ATGTAGATAT TGATCTGCAT 
ATTGATGTTA GCTTTCTTGA TGAGGAGATT GCTGTGGCAT GGGAAGTGAT TCGAACAGAA 
CCTATAATTG TCCGATTACA CTGTTCTCTT ACACAGTATT TGAATGGCCC AGTGCCCACT 
GTTGATGTCT TTCAGATTTC CACAAAAGAG AGATTTGGTC TGGGACATCA GCTGAAAAAA 
ATCATGCAGA CATTTGTTAC ACAACAGTGG AAGCAGAGCA AAGAAAAATC CAATTGCCTG 
CACAATAAGA AGCTGTCAGA GAAGAAAGTC AAGTCTCCCC TGCATTTATT TTCAACCTTG 
CGCAGGTCGC CAAGTTATCC TCCCCCTGGT TGTGGAAAAA GCAAATCTAA ACTGAAATCG 
GAGCAGGACG GGATCTCCAA GACGCACAAG CTACTGCGGA GGACTTGTTC CAGCACCGTC 
AAGACGGAGG ACGTGTGCGC CCCGAAGCCA CACAGGACCT TCGGTCGCTC GCTGTCCAGC 
GACCCCCGGG CTGAGCAGGC AGTGACAACC ATTAAGTCGC ACAAACTCTT GAACCGTCCC 
TGCTCTGCAG CCGTCAAGCA GGAGGACTGC CTTGCTCTCA AGTCGCACAA ACTCTTGACC 
CGCTCTTGTT CCGGAGACCC GCGCTGTGAG CACAATGCCA GCCTGAAGCC TCACAAACTG 
TTAAGCAGGT CTTACTCCAG TAACCTCAGA ATGGAAGAAT TATACGGACT CAAGAATCAC 
AAGTTGCTCA GCAAGTCTTA CTCCAGTGCC CCCAAGTCAT CCAAAACGGA GCTTTTCAAG 
GAACCCAACA CAGAGGGCAG GAGGCTCTCT CTTACCTCAG GGCTCATTGG TATCTTAACA 
CCATCGTCAT CTTCATCTTC CCAGCCTGCT CCAAATGGTG CCAAATGCAT TCCAATACGA 
GACCGTGGCT TCCTAGTGCA GACAATTGAG TTTGCTGAAC AGCGGATCCC TGTGTTGAAT 
GAGTACTGTG TGGTTTGTGA TGAACCACAT GTATTTCAGA ATGGCCCCAT GCTTAGGCCT 
ACGGTGTGTG AACGAGAGCT GTGTGTATTT GCTTTTCAAA CCCTGGGAGT AATGAATGAA 
GCTGCTGATG AAATCGCAAC TGGAGCTCAG GTGGTAGATC TACTAGTATC CATGTGTAGG 
TCTGCATTGG AATCTCCTAG AAAAGTTGTC ATTTTCGAGC CATATCCTTC TGTGGTAGAT 
CCTAACGATC CTCAGATGCT GGCCTTCAAC CCCAGGAAGA AGAACTATGA TCGAGTAATG 
AAAGCATTGG ATAGCATAAC TTCTATTAGA GAAATGACAC AAGCACCATA TCTGGAAATC 
AAGAAGCAGA TGGATAAACA AGACCCCCTT GCTCATCCCT TACTGCAATG GGTTATTTCA 
AGTAATAGGT CACATATTGT GAAACTGCCA GTTAACAGGC AACTGAAGTT CATGCACACA 
CCCCATCAGT TCCTTCTTCT CAGCAGTCCA CCAGCCAAAG AATCCAATTT TAGAGCTGCT 
AAAAAACTCT TCGGAAGCAC CTTTGCATTT CATGGCTCAC ACATTGAAAA CTGGCATTCC 
ATCCTGAGGA ATGGTCTGGT TGTTGCTTCT AATACACGAC TGCAGCTCCA CGGTGCAATG 
TATGGAAGTG GAATCTATCT TAGTCCAATG TCAAGCATAT CATTTGGTTA CTCAGGGATG 
AACAAGAAGC AGAAGGTGTC AGCCAAGGAT GAGCCGGCTT CAAGCAGTAA AAACAGCAAC 
GCATCACAGT CACAGAAGAA AGGACAGCAA TCCCAGTTCC TGCAAAGCCG TAACTTAAAA 
TGCATAGCCT TATGTGAAGT GATCACCTCA CCTGACCTGC ACAAACATGG AGAGATATGG 
GTCGTCCCAA ATACTGATCA TGTCTGCACA AGATTCTTTT TTGTCTATGA AGATGGCCAA 
GTGGGAGACG CAAATATTAA CACGCAAGAA GGAGGCATTC ACAAAGAGAT CCTTCGAGTA 
ATTGGTAATC AAACTGCTAC TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221592.1
CDS	179..2785
Translation	MDGKTEQSRTGIYLMGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRLATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLLAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTEDVCAPKPHRTFGRSLSSDPRAEQAVTTIKSHKLLNRPCSAAVKQEDCLALKSHKLLTRSCSGDPRCEHNASLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNTEGRRLSLTSGLIGILTPSSSSSSQPAPNGAKCIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKNSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG

Target ORF information:

RefSeq Version	XM_005221535.3
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221535.3

ORF Insert Sequence:

ATGGACGGAA AGACCGAGCA GAGCAGAACG GGGATTTATT TAATGGGGAT GTGTTCAAGG 
CAAGAGCGAA TTCAGAAGGA TATCGACGTC GTGATCCAGA AGTCCAGAGC CGAGAAGGAC 
TGCCTGTTTG CAGATTTCAG ATACTCAGAC TCCACCTTTA CTTTCACCTA CGTTGGCGGC 
CCCAAAAGTG TATCCTATTC AGTACATGTA TCTGAAGATT ACCCAGATAA TACATATGTG 
TCAAGTTCAG AAAATGATGA AGATGTATTA GTTACTACAG AGCCAATTCC AGTAATTTTT 
CATCGACTAG CAACAGAATT AAGAAAGACA AATGACATTA ACTGTTGCTT ATCCATAAAA 
TCCAAATTAC AGAAGGAAAA TGGCGAGGAG TCAAGACAGA ATAGCACAGT GGAGGAAGAC 
TCTGAAGGAG ACAATGATTC GGAAGAATTT TATTACGGAG GACAGGTTAA CTATGACGGG 
GAGCTGCACA AACACCCCCA GCTTGAAGCT GACTTGTTGG CAGTGAGAGA GATATATGGG 
CCACATGCAG TTTCTCTGAG GGAATATGGA GCCATTGATG ATGTAGATAT TGATCTGCAT 
ATTGATGTTA GCTTTCTTGA TGAGGAGATT GCTGTGGCAT GGGAAGTGAT TCGAACAGAA 
CCTATAATTG TCCGATTACA CTGTTCTCTT ACACAGTATT TGAATGGCCC AGTGCCCACT 
GTTGATGTCT TTCAGATTTC CACAAAAGAG AGATTTGGTC TGGGACATCA GCTGAAAAAA 
ATCATGCAGA CATTTGTTAC ACAACAGTGG AAGCAGAGCA AAGAAAAATC CAATTGCCTG 
CACAATAAGA AGCTGTCAGA GAAGAAAGTC AAGTCTCCCC TGCATTTATT TTCAACCTTG 
CGCAGGTCGC CAAGTTATCC TCCCCCTGGT TGTGGAAAAA GCAAATCTAA ACTGAAATCG 
GAGCAGGACG GGATCTCCAA GACGCACAAG CTACTGCGGA GGACTTGTTC CAGCACCGTC 
AAGACGGAGG ACGTGTGCGC CCCGAAGCCA CACAGGACCT TCGGTCGCTC GCTGTCCAGC 
GACCCCCGGG CTGAGCAGGC AGTGACAACC ATTAAGTCGC ACAAACTCTT GAACCGTCCC 
TGCTCTGCAG CCGTCAAGCA GGAGGACTGC CTTGCTCTCA AGTCGCACAA ACTCTTGACC 
CGCTCTTGTT CCGGAGACCC GCGCTGTGAG CACAATGCCA GCCTGAAGCC TCACAAACTG 
TTAAGCAGGT CTTACTCCAG TAACCTCAGA ATGGAAGAAT TATACGGACT CAAGAATCAC 
AAGTTGCTCA GCAAGTCTTA CTCCAGTGCC CCCAAGTCAT CCAAAACGGA GCTTTTCAAG 
GAACCCAACA CAGAGGGCAG GAGGCTCTCT CTTACCTCAG GGCTCATTGG TATCTTAACA 
CCATCGTCAT CTTCATCTTC CCAGCCTGCT CCAAATGGTG CCAAATGCAT TCCAATACGA 
GACCGTGGCT TCCTAGTGCA GACAATTGAG TTTGCTGAAC AGCGGATCCC TGTGTTGAAT 
GAGTACTGTG TGGTTTGTGA TGAACCACAT GTATTTCAGA ATGGCCCCAT GCTTAGGCCT 
ACGGTGTGTG AACGAGAGCT GTGTGTATTT GCTTTTCAAA CCCTGGGAGT AATGAATGAA 
GCTGCTGATG AAATCGCAAC TGGAGCTCAG GTGGTAGATC TACTAGTATC CATGTGTAGG 
TCTGCATTGG AATCTCCTAG AAAAGTTGTC ATTTTCGAGC CATATCCTTC TGTGGTAGAT 
CCTAACGATC CTCAGATGCT GGCCTTCAAC CCCAGGAAGA AGAACTATGA TCGAGTAATG 
AAAGCATTGG ATAGCATAAC TTCTATTAGA GAAATGACAC AAGCACCATA TCTGGAAATC 
AAGAAGCAGA TGGATAAACA AGACCCCCTT GCTCATCCCT TACTGCAATG GGTTATTTCA 
AGTAATAGGT CACATATTGT GAAACTGCCA GTTAACAGGC AACTGAAGTT CATGCACACA 
CCCCATCAGT TCCTTCTTCT CAGCAGTCCA CCAGCCAAAG AATCCAATTT TAGAGCTGCT 
AAAAAACTCT TCGGAAGCAC CTTTGCATTT CATGGCTCAC ACATTGAAAA CTGGCATTCC 
ATCCTGAGGA ATGGTCTGGT TGTTGCTTCT AATACACGAC TGCAGCTCCA CGGTGCAATG 
TATGGAAGTG GAATCTATCT TAGTCCAATG TCAAGCATAT CATTTGGTTA CTCAGGGATG 
AACAAGAAGC AGAAGGTGTC AGCCAAGGAT GAGCCGGCTT CAAGCAGTAA AAACAGCAAC 
GCATCACAGT CACAGAAGAA AGGACAGCAA TCCCAGTTCC TGCAAAGCCG TAACTTAAAA 
TGCATAGCCT TATGTGAAGT GATCACCTCA CCTGACCTGC ACAAACATGG AGAGATATGG 
GTCGTCCCAA ATACTGATCA TGTCTGCACA AGATTCTTTT TTGTCTATGA AGATGGCCAA 
GTGGGAGACG CAAATATTAA CACGCAAGAA GGAGGCATTC ACAAAGAGAT CCTTCGAGTA 
ATTGGTAATC AAACTGCTAC TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PARP8 ( XM_005221535.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005221592.1 Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.

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CloneID	OBa30629
Clone ID Related Accession (Same CDS sequence)	XM_005221535.3 , XM_005221535.4
Accession Version	XM_005221535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	poly [ADP-ribose] polymerase 8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005221535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACGGAA AGACCGAGCA GAGCAGAACG GGGATTTATT TAATGGGGAT GTGTTCAAGG 
CAAGAGCGAA TTCAGAAGGA TATCGACGTC GTGATCCAGA AGTCCAGAGC CGAGAAGGAC 
TGCCTGTTTG CAGATTTCAG ATACTCAGAC TCCACCTTTA CTTTCACCTA CGTTGGCGGC 
CCCAAAAGTG TATCCTATTC AGTACATGTA TCTGAAGATT ACCCAGATAA TACATATGTG 
TCAAGTTCAG AAAATGATGA AGATGTATTA GTTACTACAG AGCCAATTCC AGTAATTTTT 
CATCGACTAG CAACAGAATT AAGAAAGACA AATGACATTA ACTGTTGCTT ATCCATAAAA 
TCCAAATTAC AGAAGGAAAA TGGCGAGGAG TCAAGACAGA ATAGCACAGT GGAGGAAGAC 
TCTGAAGGAG ACAATGATTC GGAAGAATTT TATTACGGAG GACAGGTTAA CTATGACGGG 
GAGCTGCACA AACACCCCCA GCTTGAAGCT GACTTGTTGG CAGTGAGAGA GATATATGGG 
CCACATGCAG TTTCTCTGAG GGAATATGGA GCCATTGATG ATGTAGATAT TGATCTGCAT 
ATTGATGTTA GCTTTCTTGA TGAGGAGATT GCTGTGGCAT GGGAAGTGAT TCGAACAGAA 
CCTATAATTG TCCGATTACA CTGTTCTCTT ACACAGTATT TGAATGGCCC AGTGCCCACT 
GTTGATGTCT TTCAGATTTC CACAAAAGAG AGATTTGGTC TGGGACATCA GCTGAAAAAA 
ATCATGCAGA CATTTGTTAC ACAACAGTGG AAGCAGAGCA AAGAAAAATC CAATTGCCTG 
CACAATAAGA AGCTGTCAGA GAAGAAAGTC AAGTCTCCCC TGCATTTATT TTCAACCTTG 
CGCAGGTCGC CAAGTTATCC TCCCCCTGGT TGTGGAAAAA GCAAATCTAA ACTGAAATCG 
GAGCAGGACG GGATCTCCAA GACGCACAAG CTACTGCGGA GGACTTGTTC CAGCACCGTC 
AAGACGGAGG ACGTGTGCGC CCCGAAGCCA CACAGGACCT TCGGTCGCTC GCTGTCCAGC 
GACCCCCGGG CTGAGCAGGC AGTGACAACC ATTAAGTCGC ACAAACTCTT GAACCGTCCC 
TGCTCTGCAG CCGTCAAGCA GGAGGACTGC CTTGCTCTCA AGTCGCACAA ACTCTTGACC 
CGCTCTTGTT CCGGAGACCC GCGCTGTGAG CACAATGCCA GCCTGAAGCC TCACAAACTG 
TTAAGCAGGT CTTACTCCAG TAACCTCAGA ATGGAAGAAT TATACGGACT CAAGAATCAC 
AAGTTGCTCA GCAAGTCTTA CTCCAGTGCC CCCAAGTCAT CCAAAACGGA GCTTTTCAAG 
GAACCCAACA CAGAGGGCAG GAGGCTCTCT CTTACCTCAG GGCTCATTGG TATCTTAACA 
CCATCGTCAT CTTCATCTTC CCAGCCTGCT CCAAATGGTG CCAAATGCAT TCCAATACGA 
GACCGTGGCT TCCTAGTGCA GACAATTGAG TTTGCTGAAC AGCGGATCCC TGTGTTGAAT 
GAGTACTGTG TGGTTTGTGA TGAACCACAT GTATTTCAGA ATGGCCCCAT GCTTAGGCCT 
ACGGTGTGTG AACGAGAGCT GTGTGTATTT GCTTTTCAAA CCCTGGGAGT AATGAATGAA 
GCTGCTGATG AAATCGCAAC TGGAGCTCAG GTGGTAGATC TACTAGTATC CATGTGTAGG 
TCTGCATTGG AATCTCCTAG AAAAGTTGTC ATTTTCGAGC CATATCCTTC TGTGGTAGAT 
CCTAACGATC CTCAGATGCT GGCCTTCAAC CCCAGGAAGA AGAACTATGA TCGAGTAATG 
AAAGCATTGG ATAGCATAAC TTCTATTAGA GAAATGACAC AAGCACCATA TCTGGAAATC 
AAGAAGCAGA TGGATAAACA AGACCCCCTT GCTCATCCCT TACTGCAATG GGTTATTTCA 
AGTAATAGGT CACATATTGT GAAACTGCCA GTTAACAGGC AACTGAAGTT CATGCACACA 
CCCCATCAGT TCCTTCTTCT CAGCAGTCCA CCAGCCAAAG AATCCAATTT TAGAGCTGCT 
AAAAAACTCT TCGGAAGCAC CTTTGCATTT CATGGCTCAC ACATTGAAAA CTGGCATTCC 
ATCCTGAGGA ATGGTCTGGT TGTTGCTTCT AATACACGAC TGCAGCTCCA CGGTGCAATG 
TATGGAAGTG GAATCTATCT TAGTCCAATG TCAAGCATAT CATTTGGTTA CTCAGGGATG 
AACAAGAAGC AGAAGGTGTC AGCCAAGGAT GAGCCGGCTT CAAGCAGTAA AAACAGCAAC 
GCATCACAGT CACAGAAGAA AGGACAGCAA TCCCAGTTCC TGCAAAGCCG TAACTTAAAA 
TGCATAGCCT TATGTGAAGT GATCACCTCA CCTGACCTGC ACAAACATGG AGAGATATGG 
GTCGTCCCAA ATACTGATCA TGTCTGCACA AGATTCTTTT TTGTCTATGA AGATGGCCAA 
GTGGGAGACG CAAATATTAA CACGCAAGAA GGAGGCATTC ACAAAGAGAT CCTTCGAGTA 
ATTGGTAATC AAACTGCTAC TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005221592.1
CDS	172..2778
Translation	MDGKTEQSRTGIYLMGMCSRQERIQKDIDVVIQKSRAEKDCLFADFRYSDSTFTFTYVGGPKSVSYSVHVSEDYPDNTYVSSSENDEDVLVTTEPIPVIFHRLATELRKTNDINCCLSIKSKLQKENGEESRQNSTVEEDSEGDNDSEEFYYGGQVNYDGELHKHPQLEADLLAVREIYGPHAVSLREYGAIDDVDIDLHIDVSFLDEEIAVAWEVIRTEPIIVRLHCSLTQYLNGPVPTVDVFQISTKERFGLGHQLKKIMQTFVTQQWKQSKEKSNCLHNKKLSEKKVKSPLHLFSTLRRSPSYPPPGCGKSKSKLKSEQDGISKTHKLLRRTCSSTVKTEDVCAPKPHRTFGRSLSSDPRAEQAVTTIKSHKLLNRPCSAAVKQEDCLALKSHKLLTRSCSGDPRCEHNASLKPHKLLSRSYSSNLRMEELYGLKNHKLLSKSYSSAPKSSKTELFKEPNTEGRRLSLTSGLIGILTPSSSSSSQPAPNGAKCIPIRDRGFLVQTIEFAEQRIPVLNEYCVVCDEPHVFQNGPMLRPTVCERELCVFAFQTLGVMNEAADEIATGAQVVDLLVSMCRSALESPRKVVIFEPYPSVVDPNDPQMLAFNPRKKNYDRVMKALDSITSIREMTQAPYLEIKKQMDKQDPLAHPLLQWVISSNRSHIVKLPVNRQLKFMHTPHQFLLLSSPPAKESNFRAAKKLFGSTFAFHGSHIENWHSILRNGLVVASNTRLQLHGAMYGSGIYLSPMSSISFGYSGMNKKQKVSAKDEPASSSKNSNASQSQKKGQQSQFLQSRNLKCIALCEVITSPDLHKHGEIWVVPNTDHVCTRFFFVYEDGQVGDANINTQEGGIHKEILRVIGNQTATG

Target ORF information:

RefSeq Version	XM_005221535.4
Organism	Bos taurus(cattle)
Definition	Bos taurus poly(ADP-ribose) polymerase family member 8 (PARP8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005221535.4

ORF Insert Sequence:

ATGGACGGAA AGACCGAGCA GAGCAGAACG GGGATTTATT TAATGGGGAT GTGTTCAAGG 
CAAGAGCGAA TTCAGAAGGA TATCGACGTC GTGATCCAGA AGTCCAGAGC CGAGAAGGAC 
TGCCTGTTTG CAGATTTCAG ATACTCAGAC TCCACCTTTA CTTTCACCTA CGTTGGCGGC 
CCCAAAAGTG TATCCTATTC AGTACATGTA TCTGAAGATT ACCCAGATAA TACATATGTG 
TCAAGTTCAG AAAATGATGA AGATGTATTA GTTACTACAG AGCCAATTCC AGTAATTTTT 
CATCGACTAG CAACAGAATT AAGAAAGACA AATGACATTA ACTGTTGCTT ATCCATAAAA 
TCCAAATTAC AGAAGGAAAA TGGCGAGGAG TCAAGACAGA ATAGCACAGT GGAGGAAGAC 
TCTGAAGGAG ACAATGATTC GGAAGAATTT TATTACGGAG GACAGGTTAA CTATGACGGG 
GAGCTGCACA AACACCCCCA GCTTGAAGCT GACTTGTTGG CAGTGAGAGA GATATATGGG 
CCACATGCAG TTTCTCTGAG GGAATATGGA GCCATTGATG ATGTAGATAT TGATCTGCAT 
ATTGATGTTA GCTTTCTTGA TGAGGAGATT GCTGTGGCAT GGGAAGTGAT TCGAACAGAA 
CCTATAATTG TCCGATTACA CTGTTCTCTT ACACAGTATT TGAATGGCCC AGTGCCCACT 
GTTGATGTCT TTCAGATTTC CACAAAAGAG AGATTTGGTC TGGGACATCA GCTGAAAAAA 
ATCATGCAGA CATTTGTTAC ACAACAGTGG AAGCAGAGCA AAGAAAAATC CAATTGCCTG 
CACAATAAGA AGCTGTCAGA GAAGAAAGTC AAGTCTCCCC TGCATTTATT TTCAACCTTG 
CGCAGGTCGC CAAGTTATCC TCCCCCTGGT TGTGGAAAAA GCAAATCTAA ACTGAAATCG 
GAGCAGGACG GGATCTCCAA GACGCACAAG CTACTGCGGA GGACTTGTTC CAGCACCGTC 
AAGACGGAGG ACGTGTGCGC CCCGAAGCCA CACAGGACCT TCGGTCGCTC GCTGTCCAGC 
GACCCCCGGG CTGAGCAGGC AGTGACAACC ATTAAGTCGC ACAAACTCTT GAACCGTCCC 
TGCTCTGCAG CCGTCAAGCA GGAGGACTGC CTTGCTCTCA AGTCGCACAA ACTCTTGACC 
CGCTCTTGTT CCGGAGACCC GCGCTGTGAG CACAATGCCA GCCTGAAGCC TCACAAACTG 
TTAAGCAGGT CTTACTCCAG TAACCTCAGA ATGGAAGAAT TATACGGACT CAAGAATCAC 
AAGTTGCTCA GCAAGTCTTA CTCCAGTGCC CCCAAGTCAT CCAAAACGGA GCTTTTCAAG 
GAACCCAACA CAGAGGGCAG GAGGCTCTCT CTTACCTCAG GGCTCATTGG TATCTTAACA 
CCATCGTCAT CTTCATCTTC CCAGCCTGCT CCAAATGGTG CCAAATGCAT TCCAATACGA 
GACCGTGGCT TCCTAGTGCA GACAATTGAG TTTGCTGAAC AGCGGATCCC TGTGTTGAAT 
GAGTACTGTG TGGTTTGTGA TGAACCACAT GTATTTCAGA ATGGCCCCAT GCTTAGGCCT 
ACGGTGTGTG AACGAGAGCT GTGTGTATTT GCTTTTCAAA CCCTGGGAGT AATGAATGAA 
GCTGCTGATG AAATCGCAAC TGGAGCTCAG GTGGTAGATC TACTAGTATC CATGTGTAGG 
TCTGCATTGG AATCTCCTAG AAAAGTTGTC ATTTTCGAGC CATATCCTTC TGTGGTAGAT 
CCTAACGATC CTCAGATGCT GGCCTTCAAC CCCAGGAAGA AGAACTATGA TCGAGTAATG 
AAAGCATTGG ATAGCATAAC TTCTATTAGA GAAATGACAC AAGCACCATA TCTGGAAATC 
AAGAAGCAGA TGGATAAACA AGACCCCCTT GCTCATCCCT TACTGCAATG GGTTATTTCA 
AGTAATAGGT CACATATTGT GAAACTGCCA GTTAACAGGC AACTGAAGTT CATGCACACA 
CCCCATCAGT TCCTTCTTCT CAGCAGTCCA CCAGCCAAAG AATCCAATTT TAGAGCTGCT 
AAAAAACTCT TCGGAAGCAC CTTTGCATTT CATGGCTCAC ACATTGAAAA CTGGCATTCC 
ATCCTGAGGA ATGGTCTGGT TGTTGCTTCT AATACACGAC TGCAGCTCCA CGGTGCAATG 
TATGGAAGTG GAATCTATCT TAGTCCAATG TCAAGCATAT CATTTGGTTA CTCAGGGATG 
AACAAGAAGC AGAAGGTGTC AGCCAAGGAT GAGCCGGCTT CAAGCAGTAA AAACAGCAAC 
GCATCACAGT CACAGAAGAA AGGACAGCAA TCCCAGTTCC TGCAAAGCCG TAACTTAAAA 
TGCATAGCCT TATGTGAAGT GATCACCTCA CCTGACCTGC ACAAACATGG AGAGATATGG 
GTCGTCCCAA ATACTGATCA TGTCTGCACA AGATTCTTTT TTGTCTATGA AGATGGCCAA 
GTGGGAGACG CAAATATTAA CACGCAAGAA GGAGGCATTC ACAAAGAGAT CCTTCGAGTA 
ATTGGTAATC AAACTGCTAC TGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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PARP8 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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