MKL2 cDNA ORF clone, Bos taurus(cattle)

The following MKL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MKL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36806 NM_001192293.1
Latest version!
Bos taurus myocardin related transcription factor B (MRTFB), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa32966 XM_005224730.4
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa32968 XM_015460329.1
Latest version!
Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa36806 XM_024984794.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_024984786.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_024984788.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_024984790.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_024984787.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_010819298.3
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_010819297.3
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_015460327.1
Latest version!
Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_024984789.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48001 XM_024984793.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48001 XM_024984791.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48001 XM_015460328.2
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48001 XM_024984792.1
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48002 XM_010819300.3
Latest version!
Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
View Old Accession Versions >>
OBa48002 XM_010819300.2 Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_010819297.2 Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa32966 XM_005224730.3 Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48000 XM_010819298.2 Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa48001 XM_015460328.1 Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36806
    Clone ID Related Accession (Same CDS sequence) NM_001192293.1 , XM_024984794.1
    Accession Version NM_001192293.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-13
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000025.1. On Jul 17, 2010 this sequence version replaced XM_588800.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5721697.5961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145432, SAMN03145456 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGATTTCCA ATGCACACAG TCAGCAGATG
    GACGACCTCT TTGACATCCT CATTAAAAGC GGAGAGATTT CCCTCCCTAT AAAAGAAGAA
    CCTTCTCCTA TCTCCAAAAT GAGACCAGTG ACAGCCAGCA TCACCACAAT GCCGGTCAGC
    ACCGTGGTGT CCCGGCCACC ACCCCAGGTC CAAATGGCAC CACCCATATC CTTAGAACCT
    ATGAGCAGTC TATCTGCCAG CTTAGAGAAC CAACTCGAAG CCTTCCTGGA TGGAACCCTA
    CCTTCAGCTA ATGAAATCGC TCCCCTGCAA AGCGGCAGCG AGGACAGAGA GCCCTTCTCC
    CTGATCGAGG ACCTCCAGAA CGACCTGCTG AGCCACTCAG GCGTGCTGGA CCCCTCACAC
    TCGCCCATGG AAACGGCCGA GGCCCAGTTT GCTGCAAGTA CGCCCTGCCT GTCCCTTGAC
    CTCTCTGACT CGAACTTGGA CAACATGGAG TGGTTAGACA TTACCATGCC CGGCACGTCA
    TCGGGACTCA CACCGCTCAG CGCCACCACT CCCAGCATGT TCTCTGCCGA CTTCCTAGAC
    CCACAGGACC TGCCGCTGCC ATGGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179222.1
    CDS164..3253
    Translation

    Target ORF information:

    RefSeq Version NM_001192293.1
    Organism Bos taurus(cattle)
    Definition Bos taurus myocardin related transcription factor B (MRTFB), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192293.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGATTTCCA ATGCACACAG TCAGCAGATG
    GACGACCTCT TTGACATCCT CATTAAAAGC GGAGAGATTT CCCTCCCTAT AAAAGAAGAA
    CCTTCTCCTA TCTCCAAAAT GAGACCAGTG ACAGCCAGCA TCACCACAAT GCCGGTCAGC
    ACCGTGGTGT CCCGGCCACC ACCCCAGGTC CAAATGGCAC CACCCATATC CTTAGAACCT
    ATGAGCAGTC TATCTGCCAG CTTAGAGAAC CAACTCGAAG CCTTCCTGGA TGGAACCCTA
    CCTTCAGCTA ATGAAATCGC TCCCCTGCAA AGCGGCAGCG AGGACAGAGA GCCCTTCTCC
    CTGATCGAGG ACCTCCAGAA CGACCTGCTG AGCCACTCAG GCGTGCTGGA CCCCTCACAC
    TCGCCCATGG AAACGGCCGA GGCCCAGTTT GCTGCAAGTA CGCCCTGCCT GTCCCTTGAC
    CTCTCTGACT CGAACTTGGA CAACATGGAG TGGTTAGACA TTACCATGCC CGGCACGTCA
    TCGGGACTCA CACCGCTCAG CGCCACCACT CCCAGCATGT TCTCTGCCGA CTTCCTAGAC
    CCACAGGACC TGCCGCTGCC ATGGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32966
    Clone ID Related Accession (Same CDS sequence) XM_005224730.4 , XM_005224730.3
    Accession Version XM_005224730.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005224730.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGATT TCCAATGCAC ACAGTCAGCA GATGGACGAC
    CTCTTTGACA TCCTCATTAA AAGCGGAGAG ATTTCCCTCC CTATAAAAGA AGAACCTTCT
    CCTATCTCCA AAATGAGACC AGTGACAGCC AGCATCACCA CAATGCCGGT CAGCACCGTG
    GTGTCCCGGC CACCACCCCA GGTCCAAATG GCACCACCCA TATCCTTAGA ACCTATGAGC
    AGTCTATCTG CCAGCTTAGA GAACCAACTC GAAGCCTTCC TGGATGGAAC CCTACCTTCA
    GCTAATGAAA TCGCTCCCCT GCAAAGCGGC AGCGAGGACA GAGAGCCCTT CTCCCTGATC
    GAGGACCTCC AGAACGACCT GCTGAGCCAC TCAGGCGTGC TGGACCCCTC ACACTCGCCC
    ATGGAAACGG CCGAGGCCCA GTTTGCTGCA AGTACGCCCT GCCTGTCCCT TGACCTCTCT
    GACTCGAACT TGGACAACAT GGAGTGGTTA GACATTACCA TGCCCGGCAC GTCATCGGGA
    CTCACACCGC TCAGCGCCAC CACTCCCAGC ATGTTCTCTG CCGACTTCCT AGACCCACAG
    GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224787.1
    CDS1481..4684
    Translation

    Target ORF information:

    RefSeq Version XM_005224730.4
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224730.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGATT TCCAATGCAC ACAGTCAGCA GATGGACGAC
    CTCTTTGACA TCCTCATTAA AAGCGGAGAG ATTTCCCTCC CTATAAAAGA AGAACCTTCT
    CCTATCTCCA AAATGAGACC AGTGACAGCC AGCATCACCA CAATGCCGGT CAGCACCGTG
    GTGTCCCGGC CACCACCCCA GGTCCAAATG GCACCACCCA TATCCTTAGA ACCTATGAGC
    AGTCTATCTG CCAGCTTAGA GAACCAACTC GAAGCCTTCC TGGATGGAAC CCTACCTTCA
    GCTAATGAAA TCGCTCCCCT GCAAAGCGGC AGCGAGGACA GAGAGCCCTT CTCCCTGATC
    GAGGACCTCC AGAACGACCT GCTGAGCCAC TCAGGCGTGC TGGACCCCTC ACACTCGCCC
    ATGGAAACGG CCGAGGCCCA GTTTGCTGCA AGTACGCCCT GCCTGTCCCT TGACCTCTCT
    GACTCGAACT TGGACAACAT GGAGTGGTTA GACATTACCA TGCCCGGCAC GTCATCGGGA
    CTCACACCGC TCAGCGCCAC CACTCCCAGC ATGTTCTCTG CCGACTTCCT AGACCCACAG
    GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32966
    Clone ID Related Accession (Same CDS sequence) XM_005224730.4 , XM_005224730.3
    Accession Version XM_005224730.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005224730.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGATT TCCAATGCAC ACAGTCAGCA GATGGACGAC
    CTCTTTGACA TCCTCATTAA AAGCGGAGAG ATTTCCCTCC CTATAAAAGA AGAACCTTCT
    CCTATCTCCA AAATGAGACC AGTGACAGCC AGCATCACCA CAATGCCGGT CAGCACCGTG
    GTGTCCCGGC CACCACCCCA GGTCCAAATG GCACCACCCA TATCCTTAGA ACCTATGAGC
    AGTCTATCTG CCAGCTTAGA GAACCAACTC GAAGCCTTCC TGGATGGAAC CCTACCTTCA
    GCTAATGAAA TCGCTCCCCT GCAAAGCGGC AGCGAGGACA GAGAGCCCTT CTCCCTGATC
    GAGGACCTCC AGAACGACCT GCTGAGCCAC TCAGGCGTGC TGGACCCCTC ACACTCGCCC
    ATGGAAACGG CCGAGGCCCA GTTTGCTGCA AGTACGCCCT GCCTGTCCCT TGACCTCTCT
    GACTCGAACT TGGACAACAT GGAGTGGTTA GACATTACCA TGCCCGGCAC GTCATCGGGA
    CTCACACCGC TCAGCGCCAC CACTCCCAGC ATGTTCTCTG CCGACTTCCT AGACCCACAG
    GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005224787.1
    CDS1904..5107
    Translation

    Target ORF information:

    RefSeq Version XM_005224730.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005224730.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGATT TCCAATGCAC ACAGTCAGCA GATGGACGAC
    CTCTTTGACA TCCTCATTAA AAGCGGAGAG ATTTCCCTCC CTATAAAAGA AGAACCTTCT
    CCTATCTCCA AAATGAGACC AGTGACAGCC AGCATCACCA CAATGCCGGT CAGCACCGTG
    GTGTCCCGGC CACCACCCCA GGTCCAAATG GCACCACCCA TATCCTTAGA ACCTATGAGC
    AGTCTATCTG CCAGCTTAGA GAACCAACTC GAAGCCTTCC TGGATGGAAC CCTACCTTCA
    GCTAATGAAA TCGCTCCCCT GCAAAGCGGC AGCGAGGACA GAGAGCCCTT CTCCCTGATC
    GAGGACCTCC AGAACGACCT GCTGAGCCAC TCAGGCGTGC TGGACCCCTC ACACTCGCCC
    ATGGAAACGG CCGAGGCCCA GTTTGCTGCA AGTACGCCCT GCCTGTCCCT TGACCTCTCT
    GACTCGAACT TGGACAACAT GGAGTGGTTA GACATTACCA TGCCCGGCAC GTCATCGGGA
    CTCACACCGC TCAGCGCCAC CACTCCCAGC ATGTTCTCTG CCGACTTCCT AGACCCACAG
    GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa32968
    Clone ID Related Accession (Same CDS sequence) XM_015460329.1
    Accession Version XM_015460329.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3114bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGGTGAGGA GGCCAGGCTC CATCGTGATT
    TCCAATGCAC ACAGTCAGCA GATGGACGAC CTCTTTGACA TCCTCATTAA AAGCGGAGAG
    ATTTCCCTCC CTATAAAAGA AGAACCTTCT CCTATCTCCA AAATGAGACC AGTGACAGCC
    AGCATCACCA CAATGCCGGT CAGCACCGTG GTGTCCCGGC CACCACCCCA GGTCCAAATG
    GCACCACCCA TATCCTTAGA ACCTATGAGC AGTCTATCTG CCAGCTTAGA GAACCAACTC
    GAAGCCTTCC TGGATGGAAC CCTACCTTCA GCTAATGAAA TCGCTCCCCT GCAAAGCGGC
    AGCGAGGACA GAGAGCCCTT CTCCCTGATC GAGGACCTCC AGAACGACCT GCTGAGCCAC
    TCAGGCGTGC TGGACCCCTC ACACTCGCCC ATGGAAACGG CCGAGGCCCA GTTTGCTGCA
    AGTACGCCCT GCCTGTCCCT TGACCTCTCT GACTCGAACT TGGACAACAT GGAGTGGTTA
    GACATTACCA TGCCCGGCAC GTCATCGGGA CTCACACCGC TCAGCGCCAC CACTCCCAGC
    ATGTTCTCTG CCGACTTCCT AGACCCACAG GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315815.1
    CDS133..3246
    Translation

    Target ORF information:

    RefSeq Version XM_015460329.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460329.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGGTGAGGA GGCCAGGCTC CATCGTGATT
    TCCAATGCAC ACAGTCAGCA GATGGACGAC CTCTTTGACA TCCTCATTAA AAGCGGAGAG
    ATTTCCCTCC CTATAAAAGA AGAACCTTCT CCTATCTCCA AAATGAGACC AGTGACAGCC
    AGCATCACCA CAATGCCGGT CAGCACCGTG GTGTCCCGGC CACCACCCCA GGTCCAAATG
    GCACCACCCA TATCCTTAGA ACCTATGAGC AGTCTATCTG CCAGCTTAGA GAACCAACTC
    GAAGCCTTCC TGGATGGAAC CCTACCTTCA GCTAATGAAA TCGCTCCCCT GCAAAGCGGC
    AGCGAGGACA GAGAGCCCTT CTCCCTGATC GAGGACCTCC AGAACGACCT GCTGAGCCAC
    TCAGGCGTGC TGGACCCCTC ACACTCGCCC ATGGAAACGG CCGAGGCCCA GTTTGCTGCA
    AGTACGCCCT GCCTGTCCCT TGACCTCTCT GACTCGAACT TGGACAACAT GGAGTGGTTA
    GACATTACCA TGCCCGGCAC GTCATCGGGA CTCACACCGC TCAGCGCCAC CACTCCCAGC
    ATGTTCTCTG CCGACTTCCT AGACCCACAG GACCTGCCGC TGCCATGGGA CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa36806
    Clone ID Related Accession (Same CDS sequence) NM_001192293.1 , XM_024984794.1
    Accession Version XM_024984794.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3090bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGATTTCCA ATGCACACAG TCAGCAGATG
    GACGACCTCT TTGACATCCT CATTAAAAGC GGAGAGATTT CCCTCCCTAT AAAAGAAGAA
    CCTTCTCCTA TCTCCAAAAT GAGACCAGTG ACAGCCAGCA TCACCACAAT GCCGGTCAGC
    ACCGTGGTGT CCCGGCCACC ACCCCAGGTC CAAATGGCAC CACCCATATC CTTAGAACCT
    ATGAGCAGTC TATCTGCCAG CTTAGAGAAC CAACTCGAAG CCTTCCTGGA TGGAACCCTA
    CCTTCAGCTA ATGAAATCGC TCCCCTGCAA AGCGGCAGCG AGGACAGAGA GCCCTTCTCC
    CTGATCGAGG ACCTCCAGAA CGACCTGCTG AGCCACTCAG GCGTGCTGGA CCCCTCACAC
    TCGCCCATGG AAACGGCCGA GGCCCAGTTT GCTGCAAGTA CGCCCTGCCT GTCCCTTGAC
    CTCTCTGACT CGAACTTGGA CAACATGGAG TGGTTAGACA TTACCATGCC CGGCACGTCA
    TCGGGACTCA CACCGCTCAG CGCCACCACT CCCAGCATGT TCTCTGCCGA CTTCCTAGAC
    CCACAGGACC TGCCGCTGCC ATGGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840562.1
    CDS171..3260
    Translation

    Target ORF information:

    RefSeq Version XM_024984794.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984794.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACAC TCCACCACAA ACAGGACTCC AGACGCAGCC TCAGATAGCA
    GCAGCCACCC TGTCCTCAGG CCTGGCCCAG GCGGCGCCTC AGAAGCAAGA CGCCTTCCCA
    GCACGCACGC TCGGTCAGCC TCAGCAGGCC AGAAAGGTTT TCACCAGCTC GGCATCCAGT
    ACAGTTCTGC CCTATCAGAG ACCACCTGCT CCAGCGGTGC AGCAGCCCTT TATCAACAAG
    GCGAACAACA GCGTCCTTCA GCCCCGAAGC ACACCGCTGC CCTCCCTGCA GAACGGCCCC
    AGCCCTCCCA ACAAGCCCGG CTCGCCCCCT CCGCCCCAGC AGTTTGTGGT CCAGCACTCT
    CTGTTTGGGA ATCCAGTCCC CAAGACGAAA GACCCTCCCC GCTATGAGGA AGCCATCAAG
    CAGACGCGCA GCGCCCAGTC TTCCCTCCCA GAGATTTCCA ATGCACACAG TCAGCAGATG
    GACGACCTCT TTGACATCCT CATTAAAAGC GGAGAGATTT CCCTCCCTAT AAAAGAAGAA
    CCTTCTCCTA TCTCCAAAAT GAGACCAGTG ACAGCCAGCA TCACCACAAT GCCGGTCAGC
    ACCGTGGTGT CCCGGCCACC ACCCCAGGTC CAAATGGCAC CACCCATATC CTTAGAACCT
    ATGAGCAGTC TATCTGCCAG CTTAGAGAAC CAACTCGAAG CCTTCCTGGA TGGAACCCTA
    CCTTCAGCTA ATGAAATCGC TCCCCTGCAA AGCGGCAGCG AGGACAGAGA GCCCTTCTCC
    CTGATCGAGG ACCTCCAGAA CGACCTGCTG AGCCACTCAG GCGTGCTGGA CCCCTCACAC
    TCGCCCATGG AAACGGCCGA GGCCCAGTTT GCTGCAAGTA CGCCCTGCCT GTCCCTTGAC
    CTCTCTGACT CGAACTTGGA CAACATGGAG TGGTTAGACA TTACCATGCC CGGCACGTCA
    TCGGGACTCA CACCGCTCAG CGCCACCACT CCCAGCATGT TCTCTGCCGA CTTCCTAGAC
    CCACAGGACC TGCCGCTGCC ATGGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_024984786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840554.1
    CDS252..3512
    Translation

    Target ORF information:

    RefSeq Version XM_024984786.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_024984787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840555.1
    CDS284..3544
    Translation

    Target ORF information:

    RefSeq Version XM_024984787.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_024984789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840557.1
    CDS244..3504
    Translation

    Target ORF information:

    RefSeq Version XM_024984789.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_015460327.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315813.1
    CDS809..4069
    Translation

    Target ORF information:

    RefSeq Version XM_015460327.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460327.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_024984790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840558.1
    CDS431..3691
    Translation

    Target ORF information:

    RefSeq Version XM_024984790.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_010819297.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817599.1
    CDS206..3466
    Translation

    Target ORF information:

    RefSeq Version XM_010819297.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819297.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_010819297.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817599.1
    CDS208..3468
    Translation

    Target ORF information:

    RefSeq Version XM_010819297.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819297.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_024984788.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840556.1
    CDS212..3472
    Translation

    Target ORF information:

    RefSeq Version XM_024984788.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_010819298.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819298.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817600.1
    CDS166..3426
    Translation

    Target ORF information:

    RefSeq Version XM_010819298.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819298.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48000
    Clone ID Related Accession (Same CDS sequence) XM_024984786.1 , XM_024984787.1 , XM_024984789.1 , XM_015460327.1 , XM_024984790.1 , XM_010819297.2 , XM_010819297.3 , XM_024984788.1 , XM_010819298.2 , XM_010819298.3
    Accession Version XM_010819298.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819298.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817600.1
    CDS171..3431
    Translation

    Target ORF information:

    RefSeq Version XM_010819298.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819298.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    GTGAGGAGGC CAGGCTCCAT CGTGATTTCC AATGCACACA GTCAGCAGAT GGACGACCTC
    TTTGACATCC TCATTAAAAG CGGAGAGATT TCCCTCCCTA TAAAAGAAGA ACCTTCTCCT
    ATCTCCAAAA TGAGACCAGT GACAGCCAGC ATCACCACAA TGCCGGTCAG CACCGTGGTG
    TCCCGGCCAC CACCCCAGGT CCAAATGGCA CCACCCATAT CCTTAGAACC TATGAGCAGT
    CTATCTGCCA GCTTAGAGAA CCAACTCGAA GCCTTCCTGG ATGGAACCCT ACCTTCAGCT
    AATGAAATCG CTCCCCTGCA AAGCGGCAGC GAGGACAGAG AGCCCTTCTC CCTGATCGAG
    GACCTCCAGA ACGACCTGCT GAGCCACTCA GGCGTGCTGG ACCCCTCACA CTCGCCCATG
    GAAACGGCCG AGGCCCAGTT TGCTGCAAGT ACGCCCTGCC TGTCCCTTGA CCTCTCTGAC
    TCGAACTTGG ACAACATGGA GTGGTTAGAC ATTACCATGC CCGGCACGTC ATCGGGACTC
    ACACCGCTCA GCGCCACCAC TCCCAGCATG TTCTCTGCCG ACTTCCTAGA CCCACAGGAC
    CTGCCGCTGC CATGGGACTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48001
    Clone ID Related Accession (Same CDS sequence) XM_015460328.1 , XM_024984793.1 , XM_024984791.1 , XM_024984792.1 , XM_015460328.2
    Accession Version XM_015460328.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315814.1
    CDS133..3369
    Translation

    Target ORF information:

    RefSeq Version XM_015460328.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460328.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48001
    Clone ID Related Accession (Same CDS sequence) XM_015460328.1 , XM_024984793.1 , XM_024984791.1 , XM_024984792.1 , XM_015460328.2
    Accession Version XM_024984793.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840561.1
    CDS426..3662
    Translation

    Target ORF information:

    RefSeq Version XM_024984793.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984793.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48001
    Clone ID Related Accession (Same CDS sequence) XM_015460328.1 , XM_024984793.1 , XM_024984791.1 , XM_024984792.1 , XM_015460328.2
    Accession Version XM_024984791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840559.1
    CDS208..3444
    Translation

    Target ORF information:

    RefSeq Version XM_024984791.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48001
    Clone ID Related Accession (Same CDS sequence) XM_015460328.1 , XM_024984793.1 , XM_024984791.1 , XM_024984792.1 , XM_015460328.2
    Accession Version XM_024984792.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024840560.1
    CDS212..3448
    Translation

    Target ORF information:

    RefSeq Version XM_024984792.1
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024984792.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48001
    Clone ID Related Accession (Same CDS sequence) XM_015460328.1 , XM_024984793.1 , XM_024984791.1 , XM_024984792.1 , XM_015460328.2
    Accession Version XM_015460328.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015460328.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315814.1
    CDS188..3424
    Translation

    Target ORF information:

    RefSeq Version XM_015460328.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015460328.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATCACA CAGGGGCACT GGACACCGAG GATGAGCTGG GACCTTTAGC CCATCTCGCT 
    CCAAGTCCTC AAAGTGAAGC CGTTGCCCAC GAATTCCAGG AACTCTCCTT GCAGTCCAGT
    CAGCACCTGC CTCCTCTTAA CGAAAGGAAG AATGTGCTCC AGCTAAGGCT GCAACAAAGG
    AGGACGAGAG AGCAACTAGT GGACCAGGGC ATCATGCCAC CTCTGAAGAG CCCCGCGGCA
    TTCCATGAGC AGATAAAAAG CTTGGAACGA GCCAGAACCG AAAACTTTTT GAAGCACAAG
    ATTCGGAGTC GACCAGATCG TTCTGAACTT GTCAGGATGC ACATTTTAGA AGAAACATTT
    GCAGAGCCGT CCCTGCAGGC TACTCAGATG AAGTTGAAAA GAGCTCGACT AGCTGATGAT
    CTGAATGAAA AGATTGCTCA AAGACCTGGT CCTATGGAGC TGGTAGAGAA AAACATCCTT
    CCTGTAGACT CCAGTGTTAA GGAAGCAATT ATAGGCGTTG GGAAAGAGGA CTATCCCCAA
    ACTCAGGGCG ATTTCTCATT TGACGAAGAC AGCAGTGATG CTTTGTCTCC GGACCAGCCA
    GCCAGCCAGG AGTCGCAGGG GTCAGCCGCG TCCCCAAGTG AGCCAAAAGT TAGTGAATCG
    CCATCTCCTG TGACTACAAA CACTCCAGCC CAGTTTACTC CAGTGTCCCC AGCAGTTCCT
    GAATTCTTGA AAACTCCTAC TGCAGATCAG CCCCCGCCAC GGTCCACAGC ACCCGTCCTC
    CCCCCAAGCA CTACATCCTC AGCGAAGCCT GGGCCAGCAC TGGTGAAGCA AAGCCATCCC
    AAGAATCCAA ACGACAAACA CCGCAGCAAA AAGTGCAAAG ACCCCAAGCC CCGGGTGAAG
    AAGCTGAAGT ACCACCAGTA CATCCCGCCA GATCAGAAGG GGGAGAGGAG TGAGCCGCAC
    ATGGACTCCA ACTACGCCCG GCTGCTCCAG CAGCAGCAGC TCTTCCTGCA GCTGCAGATC
    CTCAGCCAGC AGCAGCAGCA CTACAACTAC CAGACCATCC TGCCCGCGCC ACTCAAGCCA
    CTCAATGACA AGAACAACAA CAGTGGGAAT CCAGCTTTGA ACACTACCAC ATCTAACACA
    CCAAGACAGA ATACACCTGC TCCTGTGAGA AAGCCAGGAC CTCTGCCATC GAGCTTGGAT
    GATCTAAAGG TATCGGAGCT GAAGACAGAG CTGAAGTTAA GGGGTCTCCC TGTGTCAGGC
    ACCAAACTGG ACCTCATTGA ACGCCTAAAA CCCTACCAGG AAGTGAACAG CAGCGGCCCT
    GCTGCCGGTG GCGTCACAGC CGTGCCCACA CCCGCCATCG TCCCCAGCAA CCCCGAAGTC
    ACCGTGGCCC TGCCAGTGAC AACACGACAG AACTCTGTGA CCAGCTCAGG CTCCACTTTC
    AAGGCAGAGC TGCCCCCCGC CAGCAGCAGC AGCGTGGCCC ACGTGGACAG CGGTAGCTCC
    CCACTGCCCA TCTCACCTGC ACCTTCCGAA CAGTCCAGCC TCAGTGCTGA GGACACGAGC
    ATGGCTGATA CGTTCACCGA GATCATGACC ATGATGTCCC CATCGCAGTT CCTGTGCTCG
    TCGCCCCTGC GGGCAACCAG CACTGAGGAC AGCCTCAGCC CCACCAGCAG CACGCTGTCC
    ACCCTGGAGC TGGACGCAGC CGAGAAGGAC CGCAAGCTCC AGGAGAAGGA GAAGCAGATC
    GAGGAGCTGA AGCGGAAACT GGAGCAGGAG CAGAAGCTTG TGGAAGTTCT GAAGATGCAG
    CTGGAGGTGG AGAAGCGAGG GCAGCAGCAG CAGCGGCCCC TGGAGCCCCC GCCCCCGGCC
    AGCACCCCTG CCCCTTCGGT CTCCAAGGCC GACCAGACGC GGGGCCCCAC TGCTTCCACC
    ACCAAGGACG AGGCCCCGCT CCCCGATGGC CCCGGCCCAG TGGCCGGCCA GTCTCTTGTT
    GCCAAAAAGG CCATAGTGAT CAAGCAAGAG GTGCCTGTGG CCCCGGCCGA GCCACCGAGC
    CTCGTCCCAC AGTTTTACGT GAGTTCCCAG GGCCAGCCGC CCGCCGGGGT CGCTCAGCCG
    CAGGCTTTAC TGACCACGCA AACTGCACAG CTGCTGCTCC CTGTGTCCAT CCAGGGCTCC
    AGTGTGACCT CAGTGCAGCT CCCTGCAGGC AGCCTCCAGC TCCAGACTCC ACCACAAACA
    GGACTCCAGA CGCAGCCTCA GATAGCAGCA GCCACCCTGT CCTCAGGCCT GGCCCAGGCG
    GCGCCTCAGA AGCAAGACGC CTTCCCAGCA CGCACGCTCG GTCAGCCTCA GCAGGCCAGA
    AAGGTTTTCA CCAGCTCGGC ATCCAGTACA GTTCTGCCCT ATCAGAGACC ACCTGCTCCA
    GCGGTGCAGC AGCCCTTTAT CAACAAGGCG AACAACAGCG TCCTTCAGCC CCGAAGCACA
    CCGCTGCCCT CCCTGCAGAA CGGCCCCAGC CCTCCCAACA AGCCCGGCTC GCCCCCTCCG
    CCCCAGCAGT TTGTGGTCCA GCACTCTCTG TTTGGGAATC CAGTCCCCAA GACGAAAGAC
    CCTCCCCGCT ATGAGGAAGC CATCAAGCAG ACGCGCAGCG CCCAGTCTTC CCTCCCAGAG
    ATTTCCAATG CACACAGTCA GCAGATGGAC GACCTCTTTG ACATCCTCAT TAAAAGCGGA
    GAGATTTCCC TCCCTATAAA AGAAGAACCT TCTCCTATCT CCAAAATGAG ACCAGTGACA
    GCCAGCATCA CCACAATGCC GGTCAGCACC GTGGTGTCCC GGCCACCACC CCAGGTCCAA
    ATGGCACCAC CCATATCCTT AGAACCTATG AGCAGTCTAT CTGCCAGCTT AGAGAACCAA
    CTCGAAGCCT TCCTGGATGG AACCCTACCT TCAGCTAATG AAATCGCTCC CCTGCAAAGC
    GGCAGCGAGG ACAGAGAGCC CTTCTCCCTG ATCGAGGACC TCCAGAACGA CCTGCTGAGC
    CACTCAGGCG TGCTGGACCC CTCACACTCG CCCATGGAAA CGGCCGAGGC CCAGTTTGCT
    GCAAGTACGC CCTGCCTGTC CCTTGACCTC TCTGACTCGA ACTTGGACAA CATGGAGTGG
    TTAGACATTA CCATGCCCGG CACGTCATCG GGACTCACAC CGCTCAGCGC CACCACTCCC
    AGCATGTTCT CTGCCGACTT CCTAGACCCA CAGGACCTGC CGCTGCCATG GGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48002
    Clone ID Related Accession (Same CDS sequence) XM_010819300.2 , XM_010819300.3
    Accession Version XM_010819300.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104572.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010819300.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGGTG AGGAGGCCAG GCTCCATCGT GATTTCCAAT
    GCACACAGTC AGCAGATGGA CGACCTCTTT GACATCCTCA TTAAAAGCGG AGAGATTTCC
    CTCCCTATAA AAGAAGAACC TTCTCCTATC TCCAAAATGA GACCAGTGAC AGCCAGCATC
    ACCACAATGC CGGTCAGCAC CGTGGTGTCC CGGCCACCAC CCCAGGTCCA AATGGCACCA
    CCCATATCCT TAGAACCTAT GAGCAGTCTA TCTGCCAGCT TAGAGAACCA ACTCGAAGCC
    TTCCTGGATG GAACCCTACC TTCAGCTAAT GAAATCGCTC CCCTGCAAAG CGGCAGCGAG
    GACAGAGAGC CCTTCTCCCT GATCGAGGAC CTCCAGAACG ACCTGCTGAG CCACTCAGGC
    GTGCTGGACC CCTCACACTC GCCCATGGAA ACGGCCGAGG CCCAGTTTGC TGCAAGTACG
    CCCTGCCTGT CCCTTGACCT CTCTGACTCG AACTTGGACA ACATGGAGTG GTTAGACATT
    ACCATGCCCG GCACGTCATC GGGACTCACA CCGCTCAGCG CCACCACTCC CAGCATGTTC
    TCTGCCGACT TCCTAGACCC ACAGGACCTG CCGCTGCCAT GGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817602.1
    CDS1905..5132
    Translation

    Target ORF information:

    RefSeq Version XM_010819300.2
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL/myocardin-like 2 (MKL2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819300.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGGTG AGGAGGCCAG GCTCCATCGT GATTTCCAAT
    GCACACAGTC AGCAGATGGA CGACCTCTTT GACATCCTCA TTAAAAGCGG AGAGATTTCC
    CTCCCTATAA AAGAAGAACC TTCTCCTATC TCCAAAATGA GACCAGTGAC AGCCAGCATC
    ACCACAATGC CGGTCAGCAC CGTGGTGTCC CGGCCACCAC CCCAGGTCCA AATGGCACCA
    CCCATATCCT TAGAACCTAT GAGCAGTCTA TCTGCCAGCT TAGAGAACCA ACTCGAAGCC
    TTCCTGGATG GAACCCTACC TTCAGCTAAT GAAATCGCTC CCCTGCAAAG CGGCAGCGAG
    GACAGAGAGC CCTTCTCCCT GATCGAGGAC CTCCAGAACG ACCTGCTGAG CCACTCAGGC
    GTGCTGGACC CCTCACACTC GCCCATGGAA ACGGCCGAGG CCCAGTTTGC TGCAAGTACG
    CCCTGCCTGT CCCTTGACCT CTCTGACTCG AACTTGGACA ACATGGAGTG GTTAGACATT
    ACCATGCCCG GCACGTCATC GGGACTCACA CCGCTCAGCG CCACCACTCC CAGCATGTTC
    TCTGCCGACT TCCTAGACCC ACAGGACCTG CCGCTGCCAT GGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa48002
    Clone ID Related Accession (Same CDS sequence) XM_010819300.2 , XM_010819300.3
    Accession Version XM_010819300.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3228bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product MKL/myocardin-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037352.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010819300.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGGTG AGGAGGCCAG GCTCCATCGT GATTTCCAAT
    GCACACAGTC AGCAGATGGA CGACCTCTTT GACATCCTCA TTAAAAGCGG AGAGATTTCC
    CTCCCTATAA AAGAAGAACC TTCTCCTATC TCCAAAATGA GACCAGTGAC AGCCAGCATC
    ACCACAATGC CGGTCAGCAC CGTGGTGTCC CGGCCACCAC CCCAGGTCCA AATGGCACCA
    CCCATATCCT TAGAACCTAT GAGCAGTCTA TCTGCCAGCT TAGAGAACCA ACTCGAAGCC
    TTCCTGGATG GAACCCTACC TTCAGCTAAT GAAATCGCTC CCCTGCAAAG CGGCAGCGAG
    GACAGAGAGC CCTTCTCCCT GATCGAGGAC CTCCAGAACG ACCTGCTGAG CCACTCAGGC
    GTGCTGGACC CCTCACACTC GCCCATGGAA ACGGCCGAGG CCCAGTTTGC TGCAAGTACG
    CCCTGCCTGT CCCTTGACCT CTCTGACTCG AACTTGGACA ACATGGAGTG GTTAGACATT
    ACCATGCCCG GCACGTCATC GGGACTCACA CCGCTCAGCG CCACCACTCC CAGCATGTTC
    TCTGCCGACT TCCTAGACCC ACAGGACCTG CCGCTGCCAT GGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010817602.1
    CDS1485..4712
    Translation

    Target ORF information:

    RefSeq Version XM_010819300.3
    Organism Bos taurus(cattle)
    Definition Bos taurus MKL1/myocardin like 2 (MKL2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010819300.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGATCGATA GCTCCAAGAA GCAGCAACAG GGCTTCCCGG AGATTTTAAC TGCTGGCGAT 
    TTTGAGCCAC TCAAAGAAAA GGAATGCCTT GAAGGGAGCC ACCAGAAAAG TCTCAAGGAA
    GTGCTCCAGC TAAGGCTGCA ACAAAGGAGG ACGAGAGAGC AACTAGTGGA CCAGGGCATC
    ATGCCACCTC TGAAGAGCCC CGCGGCATTC CATGAGCAGA TAAAAAGCTT GGAACGAGCC
    AGAACCGAAA ACTTTTTGAA GCACAAGATT CGGAGTCGAC CAGATCGTTC TGAACTTGTC
    AGGATGCACA TTTTAGAAGA AACATTTGCA GAGCCGTCCC TGCAGGCTAC TCAGATGAAG
    TTGAAAAGAG CTCGACTAGC TGATGATCTG AATGAAAAGA TTGCTCAAAG ACCTGGTCCT
    ATGGAGCTGG TAGAGAAAAA CATCCTTCCT GTAGACTCCA GTGTTAAGGA AGCAATTATA
    GGCGTTGGGA AAGAGGACTA TCCCCAAACT CAGGGCGATT TCTCATTTGA CGAAGACAGC
    AGTGATGCTT TGTCTCCGGA CCAGCCAGCC AGCCAGGAGT CGCAGGGGTC AGCCGCGTCC
    CCAAGTGAGC CAAAAGTTAG TGAATCGCCA TCTCCTGTGA CTACAAACAC TCCAGCCCAG
    TTTACTCCAG TGTCCCCAGC AGTTCCTGAA TTCTTGAAAA CTCCTACTGC AGATCAGCCC
    CCGCCACGGT CCACAGCACC CGTCCTCCCC CCAAGCACTA CATCCTCAGC GAAGCCTGGG
    CCAGCACTGG TGAAGCAAAG CCATCCCAAG AATCCAAACG ACAAACACCG CAGCAAAAAG
    TGCAAAGACC CCAAGCCCCG GGTGAAGAAG CTGAAGTACC ACCAGTACAT CCCGCCAGAT
    CAGAAGGGGG AGAGGAGTGA GCCGCACATG GACTCCAACT ACGCCCGGCT GCTCCAGCAG
    CAGCAGCTCT TCCTGCAGCT GCAGATCCTC AGCCAGCAGC AGCAGCACTA CAACTACCAG
    ACCATCCTGC CCGCGCCACT CAAGCCACTC AATGACAAGA ACAACAACAG TGGGAATCCA
    GCTTTGAACA CTACCACATC TAACACACCA AGACAGAATA CACCTGCTCC TGTGAGAAAG
    CCAGGACCTC TGCCATCGAG CTTGGATGAT CTAAAGGTAT CGGAGCTGAA GACAGAGCTG
    AAGTTAAGGG GTCTCCCTGT GTCAGGCACC AAACTGGACC TCATTGAACG CCTAAAACCC
    TACCAGGAAG TGAACAGCAG CGGCCCTGCT GCCGGTGGCG TCACAGCCGT GCCCACACCC
    GCCATCGTCC CCAGCAACCC CGAAGTCACC GTGGCCCTGC CAGTGACAAC ACGACAGAAC
    TCTGTGACCA GCTCAGGCTC CACTTTCAAG GCAGAGCTGC CCCCCGCCAG CAGCAGCAGC
    GTGGCCCACG TGGACAGCGG TAGCTCCCCA CTGCCCATCT CACCTGCACC TTCCGAACAG
    TCCAGCCTCA GTGCTGAGGA CACGAGCATG GCTGATACGT TCACCGAGAT CATGACCATG
    ATGTCCCCAT CGCAGTTCCT GTGCTCGTCG CCCCTGCGGG CAACCAGCAC TGAGGACAGC
    CTCAGCCCCA CCAGCAGCAC GCTGTCCACC CTGGAGCTGG ACGCAGCCGA GAAGGACCGC
    AAGCTCCAGG AGAAGGAGAA GCAGATCGAG GAGCTGAAGC GGAAACTGGA GCAGGAGCAG
    AAGCTTGTGG AAGTTCTGAA GATGCAGCTG GAGGTGGAGA AGCGAGGGCA GCAGCAGCAG
    CGGCCCCTGG AGCCCCCGCC CCCGGCCAGC ACCCCTGCCC CTTCGGTCTC CAAGGCCGAC
    CAGACGCGGG GCCCCACTGC TTCCACCACC AAGGACGAGG CCCCGCTCCC CGATGGCCCC
    GGCCCAGTGG CCGGCCAGTC TCTTGTTGCC AAAAAGGCCA TAGTGATCAA GCAAGAGGTG
    CCTGTGGCCC CGGCCGAGCC ACCGAGCCTC GTCCCACAGT TTTACGTGAG TTCCCAGGGC
    CAGCCGCCCG CCGGGGTCGC TCAGCCGCAG GCTTTACTGA CCACGCAAAC TGCACAGCTG
    CTGCTCCCTG TGTCCATCCA GGGCTCCAGT GTGACCTCAG TGCAGCTCCC TGCAGGCAGC
    CTCCAGCTCC AGACTCCACC ACAAACAGGA CTCCAGACGC AGCCTCAGAT AGCAGCAGCC
    ACCCTGTCCT CAGGCCTGGC CCAGGCGGCG CCTCAGAAGC AAGACGCCTT CCCAGCACGC
    ACGCTCGGTC AGCCTCAGCA GGCCAGAAAG GTTTTCACCA GCTCGGCATC CAGTACAGTT
    CTGCCCTATC AGAGACCACC TGCTCCAGCG GTGCAGCAGC CCTTTATCAA CAAGGCGAAC
    AACAGCGTCC TTCAGCCCCG AAGCACACCG CTGCCCTCCC TGCAGAACGG CCCCAGCCCT
    CCCAACAAGC CCGGCTCGCC CCCTCCGCCC CAGCAGTTTG TGGTCCAGCA CTCTCTGTTT
    GGGAATCCAG TCCCCAAGAC GAAAGACCCT CCCCGCTATG AGGAAGCCAT CAAGCAGACG
    CGCAGCGCCC AGTCTTCCCT CCCAGAGGTG AGGAGGCCAG GCTCCATCGT GATTTCCAAT
    GCACACAGTC AGCAGATGGA CGACCTCTTT GACATCCTCA TTAAAAGCGG AGAGATTTCC
    CTCCCTATAA AAGAAGAACC TTCTCCTATC TCCAAAATGA GACCAGTGAC AGCCAGCATC
    ACCACAATGC CGGTCAGCAC CGTGGTGTCC CGGCCACCAC CCCAGGTCCA AATGGCACCA
    CCCATATCCT TAGAACCTAT GAGCAGTCTA TCTGCCAGCT TAGAGAACCA ACTCGAAGCC
    TTCCTGGATG GAACCCTACC TTCAGCTAAT GAAATCGCTC CCCTGCAAAG CGGCAGCGAG
    GACAGAGAGC CCTTCTCCCT GATCGAGGAC CTCCAGAACG ACCTGCTGAG CCACTCAGGC
    GTGCTGGACC CCTCACACTC GCCCATGGAA ACGGCCGAGG CCCAGTTTGC TGCAAGTACG
    CCCTGCCTGT CCCTTGACCT CTCTGACTCG AACTTGGACA ACATGGAGTG GTTAGACATT
    ACCATGCCCG GCACGTCATC GGGACTCACA CCGCTCAGCG CCACCACTCC CAGCATGTTC
    TCTGCCGACT TCCTAGACCC ACAGGACCTG CCGCTGCCAT GGGACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.