MDC1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_015468919.1	XP_015324405.1	mediator of DNA damage checkpoint protein 1
XM_024984123.1	XP_024839891.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_024984125.1	XP_024839893.1	mediator of DNA damage checkpoint protein 1 isoform X2
XM_024984127.1	XP_024839895.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_024984129.1	XP_024839897.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_002697355.3	XP_002697401.2	mediator of DNA damage checkpoint protein 1
XM_024984121.1	XP_024839889.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_024984124.1	XP_024839892.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_024984128.1	XP_024839896.1	mediator of DNA damage checkpoint protein 1 isoform X4
XM_024984131.1	XP_024839899.1	mediator of DNA damage checkpoint protein 1 isoform X5
XM_024984122.1	XP_024839890.1	mediator of DNA damage checkpoint protein 1 isoform X1
XM_024984126.1	XP_024839894.1	mediator of DNA damage checkpoint protein 1 isoform X3
XM_024984130.1	XP_024839898.1	mediator of DNA damage checkpoint protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229827	XM_024984124.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229827	XM_024984123.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229827	XM_024984122.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229827	XM_024984121.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229828	XM_024984125.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229829	XM_024984127.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229829	XM_024984126.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229830	XM_024984129.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229830	XM_024984130.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229830	XM_024984128.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229831	XM_024984131.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa32194	XM_002697355.3 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OBa32194	XM_015468919.1 Latest version!	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00

MDC1 ( XM_024984123.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839891.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa229827
Clone ID Related Accession (Same CDS sequence)	XM_024984123.1 , XM_024984121.1 , XM_024984124.1 , XM_024984122.1
Accession Version	XM_024984123.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839891.1
CDS	160..5937
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984123.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984123.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984121.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839889.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

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CloneID	OBa229827
Clone ID Related Accession (Same CDS sequence)	XM_024984123.1 , XM_024984121.1 , XM_024984124.1 , XM_024984122.1
Accession Version	XM_024984121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839889.1
CDS	202..5979
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984121.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984121.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984124.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839892.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa229827
Clone ID Related Accession (Same CDS sequence)	XM_024984123.1 , XM_024984121.1 , XM_024984124.1 , XM_024984122.1
Accession Version	XM_024984124.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839892.1
CDS	198..5975
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984124.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984124.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984122.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839890.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

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CloneID	OBa229827
Clone ID Related Accession (Same CDS sequence)	XM_024984123.1 , XM_024984121.1 , XM_024984124.1 , XM_024984122.1
Accession Version	XM_024984122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839890.1
CDS	347..6124
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984122.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984122.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTACCC AAAGAGAAAC AGCAGAGAGG GTGATCCCTG AGGGAGGGCC ACCGCAGAAT 
GAAACCAAGA AACTGCCCTC GGAAGGAGAG AGGGAAGATG TGACGGGAGA GGAAGAATTA 
ATCGGGGGGA TACAGGGTAG AGAACAAAAT CAGGTGTTAG CTAGAGATAC TCAGAGCCAA 
GAATCTGACA AAACAGTGAA AAGTGCAAGT ACTGGAAGGG GAATGGAGAT TGTAAAGTTA 
GAAACTGAGA CACCCAAGGA AACACAGGAG AAAGAGAGAG AAAAGCAGAC TCTCGCAGGG 
GAAATATTTG AGAGTGAAGC AGGGAAACTG GTAGCAGAGA GAGAGAGTGA GGTAGGTGGG 
TTAGAAGTCA AGGGACCCCA AGAGCTACTG GACAGAGGCC CACAGATGAG GGAGACAGAG 
GCGGGGGGCC AGGACCAGAA AGGCCAAGCC TCTGGTCCGC CATCAGAGCC TGGAGCAGGG 
GCAGGAGACC TTCAGGGATT TACTTCAGAT CCAGTAGCTT CTGGGAGCCA GGCAGGTGGA 
GGAAGGGGAG CCCCAGGGAG CCCCAGGAGG CAGCAGAGAG GTGACTTGAA TTTCGAGATG 
CCACCTGCTG AGAAGGCTTC CAGGGGTGAT CAGGAATCCC CAGATGCTTG TCTGCCTCCT 
GCAGCGCCTG AAGCCTCAGC CGCACTCCCA AACTCCCTCA TCTCTCAGAT GCAAAAACAT 
CCCGCACCTC AGTCCCTCCT TTTTCCCTCT CCAGCTCCTT TAGAACTGCC TATTCCCAGG 
ACCAGACGAA ATGAGAGTCA GGAAGCTCCA GAGACTCCCT TCTCCTCAGA GCTGAACTCT 
GTCCACCCAG AACCCAAAGT CAGGCCCCAG GGGTCCTCTC CAGTTTCTTC TCTACCCCTT 
GAGCCCCACC CTGCCACCCC CACAGGCCAG CCTATTGCCC TTGAGCCCAC ATCTGGGGTC 
TCTGGGAGCG GGACACATAG TTCCTTTGAT GTAACTGCCT CATCAGTTGT CCCCACAGCC 
CTTGCACTGC AGCCATCCAC CTCCACAGAC CAGCCTGTCA CCCCTAAGCC CACACTTGGG 
GCCCCTCGGG GCAGGGCACA GAGGTCTTCT GTCAAAACCC CTGAACCCAG TGTCCGCATA 
GACCAGCCTA TTGCCCCTGA GCTCACAGCT AAGGCCACTC GGGGCAGGGC ACAGAGGTCT 
TCTGTCAAGA CTCCCAAACC AGATAACTCC ACAACACCCA AGCCCCAGCC TTCCACTTCC 
ACAGACCAGC CTGTCACCCC CAAGCCCACA TCTCAGGCCC CTCGGGGCAG GACACCTAAG 
TCTTCTGCCA AGACTCCTGA ACCAGCTGTC CCCACAGCCT CTGAGCTCCA GCCTGCTGCC 
CCTAAAGACC AGCCTGTTGC TCCTGAGCTC ACATCTAGAG CCACTCGGGG CAGGACACAG 
AGGTCTTCTA TCAAGACTTC CAAACCAGAT ACGTCCACAG CCCCGAAGCC CCAGCCTTCC 
ACTTCCACAG ACCAGCCTGT CACCCCCAAA CCCACGTCTC GGGCCTCTCG AGGCAGGACA 
CCTAGGTCTT CCACCAAGAC TCCTGAACCA GTTGTCTCCA CAGCCTCTGA GCTCCAGCCT 
TCTGCCCTCA CAGACCAGCC TATCACTCCT GAGCTCACAT CTAGGGCTAC TCGGGGCAGG 
GCACAGAGAT CCTCTGTCAA AACCCCTGAT CCAGTTACCA CCACAACACC TGAGCTCCAG 
CCTTCCACCT CCACAGACCA GCTTGTCACC CCCAAACGCC CATCTCGGGC CCCTCGAGGC 
AGGACACGTA GGTCGTCTGC CAAGACTCCT GAACCAGTTG TTCCCACAGC CTCTGAGCTC 
CAGCCTTCTG CCCCTGCAGA CCAGCCTGTT GGTCCTTGGG CCACTCAGTG TAGAAGACAT 
AGGTCTTCTG TCAAGACCCC GGAACCAGTT GTCCCCACAG TCCCTGAACC TCAGCCTTCT 
ACTTCTAAAG ACCAGTCTGT CGCTCCTGAA CCCACATCTC AGGCCACTCA GAGCCAAACA 
CATAGGTCTT CTGTCAAGAC ATCCCAGCCA ACTGAACCCA CAGCCCCTGA CCTCAAACCT 
TCCTCCCCCA CAGACCAGCC TGTCACTCCC AAGGTCATAG CTCAGGGTGG TCCAAGCAGG 
ACACGAAGGG CTTCTACAGC AAGTGCTGTG CTGGTTCCTA CTACCCCTGA ATTCCAGTCT 
CCAGTCCGCT CAGAACAGCC TCTTTCCCCT GACCCCATCC CCGAAGTCAA CTGCAGCCTG 
AGGCCGAGGG CCACTAGGAG ACATGGGTCT CCCACAGCTC ATGTTCATGA GCCCTGCACC 
ACACCCCCTG AACCTAACTC TCGCTCCTCA AGGAACCAAA CACATGGGGC AATGAAGGCA 
GCCAAGCCCC TTAGCACCAT TTCTGAGCCT GCCTTTGCCC AGCTCCCTGA GGCACCGCCT 
CACACTCCCC AGATGCCAAA GGAGGAGGCA GCTGATGGGT CAGGCTTCAC CCCAGAGCCC 
CAGCCTAGGG CCTCTCAAAA CCGCAAGAGG CCTTCAGCTA CTGCACATTC ACCTCCACTT 
CAAAAACGGC TCCAGAGAGG GAGAGTCCCT CAGAAGGCAG CATCCCTTAA GGAAGAAGAA 
AATCCAGCAG TAAGGCCGAG GAAGGAAGAG GGTGTAGTGA TTCCAGGTCC AGGCAAGAGA 
AGGAGAGAGC AGACAGAGGA AGAGTCCCAG GGAAGACCGA GCCGTAGCCT GCGACGGACC 
AAACCTGTCC AGGAGTCCAC GGCCCCCAAA GTGCTCTTCA CGGGCGTGGT GGATGCTCGC 
GGAGAGAGGA CGGTGCTGGC CCTGGGGGGC AGTCTGGCCA GCTCAGTGGC TGAGGCTTCT 
CACCTGGTGA CTGATCGGAT CCGCCGTACG GTCAAGTTTC TGTGTGCCCT GGGCCGGGGC 
ATCCCCATCC TCTCCCTGGC CTGGCTGCAT GAGTCCCGCA AGGCAGGCTG CTTCTTGCCC 
CCGGACGAAT ATTTGGTGAC TGATCCTGAG CAGGAGAAGA ACTTCGGCTT CAGCCTTCGG 
GAGGCCCTGA GCCGAGCTCG GGAGCGAAGG CTGCTGGAGG GCTATGAGAT TCACGTGACC 
CCCGGAGTCC AGCCACCGCC ACCTCAGATG GGAGAAATCA TCAACTGCTG TGGAGGCGCC 
ATCCTGCCCA GCATGCCTCG GTCCTACAAG CCTCAGAGGG TCGTGATCAC ATGTTCCCAG 
GACTTCCCTC GATGTGCCGT TCCATATCGG GTTGGGCTGC CTGTCCTCTC ACCCGAGTTC 
CTGCTGACGG GAGTACTGAA GCAGGAAGTC AAGCCAGAGG CCTTTGCCTT CTCCACTGTG 
GAAATGTCAT CCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984125.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839893.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

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CloneID	OBa229828
Clone ID Related Accession (Same CDS sequence)	XM_024984125.1
Accession Version	XM_024984125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC CCCCAGCATG 
GAGGATGAGC CCACCCAGGC CTTCCTGTTT ACTCTGCCCC AAGAGCCTGG CCCTTCCTGT 
TGCAGCTCCC AGGCCACAGG TTCCCTGAAT GAGGCATGGG AGGTCTTGGC GACACAGCCA 
TTCTGTCCGA GAGAGTATGA GGCCTCCGAG ACCCAAACCG CTGTCACCCT CCTTGACACC 
CGTGCATCTT GCCCCCCTCC ATCTAGGACA GCACAGCAAG AGCAACATTC AGAGAGCCCA 
GTCCATGCAG AAGCACTGGG GATGGAAGGC AGAGGGATGC AGACCGTGGA GAAAGACATG 
GGTACCCAAA GAGAAACAGC AGAGAGGGTG ATCCCTGAGG GAGGGCCACC GCAGAATGAA 
ACCAAGAAAC TGCCCTCGGA AGGAGAGAGG GAAGATGTGA CGGGAGAGGA AGAATTAATC 
GGGGGGATAC AGGGTAGAGA ACAAAATCAG GTGTTAGCTA GAGATACTCA GAGCCAAGAA 
TCTGACAAAA CAGTGAAAAG TGCAAGTACT GGAAGGGGAA TGGAGATTGT AAAGTTAGAA 
ACTGAGACAC CCAAGGAAAC ACAGGAGAAA GAGAGAGAAA AGCAGACTCT CGCAGGGGAA 
ATATTTGAGA GTGAAGCAGG GAAACTGGTA GCAGAGAGAG AGAGTGAGGT AGGTGGGTTA 
GAAGTCAAGG GACCCCAAGA GCTACTGGAC AGAGGCCCAC AGATGAGGGA GACAGAGGCG 
GGGGGCCAGG ACCAGAAAGG CCAAGCCTCT GGTCCGCCAT CAGAGCCTGG AGCAGGGGCA 
GGAGACCTTC AGGGATTTAC TTCAGATCCA GTAGCTTCTG GGAGCCAGGC AGGTGGAGGA 
AGGGGAGCCC CAGGGAGCCC CAGGAGGCAG CAGAGAGGTG ACTTGAATTT CGAGATGCCA 
CCTGCTGAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAC TCCCTCATCT CTCAGATGCA AAAACATCCC 
GCACCTCAGT CCCTCCTTTT TCCCTCTCCA GCTCCTTTAG AACTGCCTAT TCCCAGGACC 
AGACGAAATG AGAGTCAGGA AGCTCCAGAG ACTCCCTTCT CCTCAGAGCT GAACTCTGTC 
CACCCAGAAC CCAAAGTCAG GCCCCAGGGG TCCTCTCCAG TTTCTTCTCT ACCCCTTGAG 
CCCCACCCTG CCACCCCCAC AGGCCAGCCT ATTGCCCTTG AGCCCACATC TGGGGTCTCT 
GGGAGCGGGA CACATAGTTC CTTTGATGTA ACTGCCTCAT CAGTTGTCCC CACAGCCCTT 
GCACTGCAGC CATCCACCTC CACAGACCAG CCTGTCACCC CTAAGCCCAC ACTTGGGGCC 
CCTCGGGGCA GGGCACAGAG GTCTTCTGTC AAAACCCCTG AACCCAGTGT CCGCATAGAC 
CAGCCTATTG CCCCTGAGCT CACAGCTAAG GCCACTCGGG GCAGGGCACA GAGGTCTTCT 
GTCAAGACTC CCAAACCAGA TAACTCCACA ACACCCAAGC CCCAGCCTTC CACTTCCACA 
GACCAGCCTG TCACCCCCAA GCCCACATCT CAGGCCCCTC GGGGCAGGAC ACCTAAGTCT 
TCTGCCAAGA CTCCTGAACC AGCTGTCCCC ACAGCCTCTG AGCTCCAGCC TGCTGCCCCT 
AAAGACCAGC CTGTTGCTCC TGAGCTCACA TCTAGAGCCA CTCGGGGCAG GACACAGAGG 
TCTTCTATCA AGACTTCCAA ACCAGATACG TCCACAGCCC CGAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCTCTCGAGG CAGGACACCT 
AGGTCTTCCA CCAAGACTCC TGAACCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ACCAGCCTAT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGCAGGGCA 
CAGAGATCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCCCAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT CGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCGGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCTACT 
TCTAAAGACC AGTCTGTCGC TCCTGAACCC ACATCTCAGG CCACTCAGAG CCAAACACAT 
AGGTCTTCTG TCAAGACATC CCAGCCAACT GAACCCACAG CCCCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCCTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
CGAAGGGCTT CTACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCGCTCAG AACAGCCTCT TTCCCCTGAC CCCATCCCCG AAGTCAACTG CAGCCTGAGG 
CCGAGGGCCA CTAGGAGACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCACA 
CCCCCTGAAC CTAACTCTCG CTCCTCAAGG AACCAAACAC ATGGGGCAAT GAAGGCAGCC 
AAGCCCCTTA GCACCATTTC TGAGCCTGCC TTTGCCCAGC TCCCTGAGGC ACCGCCTCAC 
ACTCCCCAGA TGCCAAAGGA GGAGGCAGCT GATGGGTCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCG CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGCAGTAA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAGG 
AGAGAGCAGA CAGAGGAAGA GTCCCAGGGA AGACCGAGCC GTAGCCTGCG ACGGACCAAA 
CCTGTCCAGG AGTCCACGGC CCCCAAAGTG CTCTTCACGG GCGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT CTGGCCAGCT CAGTGGCTGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGTACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCGGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA CGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA ACTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCTCGGTC CTACAAGCCT CAGAGGGTCG TGATCACATG TTCCCAGGAC 
TTCCCTCGAT GTGCCGTTCC ATATCGGGTT GGGCTGCCTG TCCTCTCACC CGAGTTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839893.1
CDS	202..5976
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984125.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984125.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC CCCCAGCATG 
GAGGATGAGC CCACCCAGGC CTTCCTGTTT ACTCTGCCCC AAGAGCCTGG CCCTTCCTGT 
TGCAGCTCCC AGGCCACAGG TTCCCTGAAT GAGGCATGGG AGGTCTTGGC GACACAGCCA 
TTCTGTCCGA GAGAGTATGA GGCCTCCGAG ACCCAAACCG CTGTCACCCT CCTTGACACC 
CGTGCATCTT GCCCCCCTCC ATCTAGGACA GCACAGCAAG AGCAACATTC AGAGAGCCCA 
GTCCATGCAG AAGCACTGGG GATGGAAGGC AGAGGGATGC AGACCGTGGA GAAAGACATG 
GGTACCCAAA GAGAAACAGC AGAGAGGGTG ATCCCTGAGG GAGGGCCACC GCAGAATGAA 
ACCAAGAAAC TGCCCTCGGA AGGAGAGAGG GAAGATGTGA CGGGAGAGGA AGAATTAATC 
GGGGGGATAC AGGGTAGAGA ACAAAATCAG GTGTTAGCTA GAGATACTCA GAGCCAAGAA 
TCTGACAAAA CAGTGAAAAG TGCAAGTACT GGAAGGGGAA TGGAGATTGT AAAGTTAGAA 
ACTGAGACAC CCAAGGAAAC ACAGGAGAAA GAGAGAGAAA AGCAGACTCT CGCAGGGGAA 
ATATTTGAGA GTGAAGCAGG GAAACTGGTA GCAGAGAGAG AGAGTGAGGT AGGTGGGTTA 
GAAGTCAAGG GACCCCAAGA GCTACTGGAC AGAGGCCCAC AGATGAGGGA GACAGAGGCG 
GGGGGCCAGG ACCAGAAAGG CCAAGCCTCT GGTCCGCCAT CAGAGCCTGG AGCAGGGGCA 
GGAGACCTTC AGGGATTTAC TTCAGATCCA GTAGCTTCTG GGAGCCAGGC AGGTGGAGGA 
AGGGGAGCCC CAGGGAGCCC CAGGAGGCAG CAGAGAGGTG ACTTGAATTT CGAGATGCCA 
CCTGCTGAGA AGGCTTCCAG GGGTGATCAG GAATCCCCAG ATGCTTGTCT GCCTCCTGCA 
GCGCCTGAAG CCTCAGCCGC ACTCCCAAAC TCCCTCATCT CTCAGATGCA AAAACATCCC 
GCACCTCAGT CCCTCCTTTT TCCCTCTCCA GCTCCTTTAG AACTGCCTAT TCCCAGGACC 
AGACGAAATG AGAGTCAGGA AGCTCCAGAG ACTCCCTTCT CCTCAGAGCT GAACTCTGTC 
CACCCAGAAC CCAAAGTCAG GCCCCAGGGG TCCTCTCCAG TTTCTTCTCT ACCCCTTGAG 
CCCCACCCTG CCACCCCCAC AGGCCAGCCT ATTGCCCTTG AGCCCACATC TGGGGTCTCT 
GGGAGCGGGA CACATAGTTC CTTTGATGTA ACTGCCTCAT CAGTTGTCCC CACAGCCCTT 
GCACTGCAGC CATCCACCTC CACAGACCAG CCTGTCACCC CTAAGCCCAC ACTTGGGGCC 
CCTCGGGGCA GGGCACAGAG GTCTTCTGTC AAAACCCCTG AACCCAGTGT CCGCATAGAC 
CAGCCTATTG CCCCTGAGCT CACAGCTAAG GCCACTCGGG GCAGGGCACA GAGGTCTTCT 
GTCAAGACTC CCAAACCAGA TAACTCCACA ACACCCAAGC CCCAGCCTTC CACTTCCACA 
GACCAGCCTG TCACCCCCAA GCCCACATCT CAGGCCCCTC GGGGCAGGAC ACCTAAGTCT 
TCTGCCAAGA CTCCTGAACC AGCTGTCCCC ACAGCCTCTG AGCTCCAGCC TGCTGCCCCT 
AAAGACCAGC CTGTTGCTCC TGAGCTCACA TCTAGAGCCA CTCGGGGCAG GACACAGAGG 
TCTTCTATCA AGACTTCCAA ACCAGATACG TCCACAGCCC CGAAGCCCCA GCCTTCCACT 
TCCACAGACC AGCCTGTCAC CCCCAAACCC ACGTCTCGGG CCTCTCGAGG CAGGACACCT 
AGGTCTTCCA CCAAGACTCC TGAACCAGTT GTCTCCACAG CCTCTGAGCT CCAGCCTTCT 
GCCCTCACAG ACCAGCCTAT CACTCCTGAG CTCACATCTA GGGCTACTCG GGGCAGGGCA 
CAGAGATCCT CTGTCAAAAC CCCTGATCCA GTTACCACCA CAACACCTGA GCTCCAGCCT 
TCCACCTCCA CAGACCAGCT TGTCACCCCC AAACGCCCAT CTCGGGCCCC TCGAGGCAGG 
ACACGTAGGT CGTCTGCCAA GACTCCTGAA CCAGTTGTTC CCACAGCCTC TGAGCTCCAG 
CCTTCTGCCC CTGCAGACCA GCCTGTTGGT CCTTGGGCCA CTCAGTGTAG AAGACATAGG 
TCTTCTGTCA AGACCCCGGA ACCAGTTGTC CCCACAGTCC CTGAACCTCA GCCTTCTACT 
TCTAAAGACC AGTCTGTCGC TCCTGAACCC ACATCTCAGG CCACTCAGAG CCAAACACAT 
AGGTCTTCTG TCAAGACATC CCAGCCAACT GAACCCACAG CCCCTGACCT CAAACCTTCC 
TCCCCCACAG ACCAGCCTGT CACTCCCAAG GTCATAGCTC AGGGTGGTCC AAGCAGGACA 
CGAAGGGCTT CTACAGCAAG TGCTGTGCTG GTTCCTACTA CCCCTGAATT CCAGTCTCCA 
GTCCGCTCAG AACAGCCTCT TTCCCCTGAC CCCATCCCCG AAGTCAACTG CAGCCTGAGG 
CCGAGGGCCA CTAGGAGACA TGGGTCTCCC ACAGCTCATG TTCATGAGCC CTGCACCACA 
CCCCCTGAAC CTAACTCTCG CTCCTCAAGG AACCAAACAC ATGGGGCAAT GAAGGCAGCC 
AAGCCCCTTA GCACCATTTC TGAGCCTGCC TTTGCCCAGC TCCCTGAGGC ACCGCCTCAC 
ACTCCCCAGA TGCCAAAGGA GGAGGCAGCT GATGGGTCAG GCTTCACCCC AGAGCCCCAG 
CCTAGGGCCT CTCAAAACCG CAAGAGGCCT TCAGCTACTG CACATTCACC TCCACTTCAA 
AAACGGCTCC AGAGAGGGAG AGTCCCTCAG AAGGCAGCAT CCCTTAAGGA AGAAGAAAAT 
CCAGCAGTAA GGCCGAGGAA GGAAGAGGGT GTAGTGATTC CAGGTCCAGG CAAGAGAAGG 
AGAGAGCAGA CAGAGGAAGA GTCCCAGGGA AGACCGAGCC GTAGCCTGCG ACGGACCAAA 
CCTGTCCAGG AGTCCACGGC CCCCAAAGTG CTCTTCACGG GCGTGGTGGA TGCTCGCGGA 
GAGAGGACGG TGCTGGCCCT GGGGGGCAGT CTGGCCAGCT CAGTGGCTGA GGCTTCTCAC 
CTGGTGACTG ATCGGATCCG CCGTACGGTC AAGTTTCTGT GTGCCCTGGG CCGGGGCATC 
CCCATCCTCT CCCTGGCCTG GCTGCATGAG TCCCGCAAGG CAGGCTGCTT CTTGCCCCCG 
GACGAATATT TGGTGACTGA TCCTGAGCAG GAGAAGAACT TCGGCTTCAG CCTTCGGGAG 
GCCCTGAGCC GAGCTCGGGA GCGAAGGCTG CTGGAGGGCT ATGAGATTCA CGTGACCCCC 
GGAGTCCAGC CACCGCCACC TCAGATGGGA GAAATCATCA ACTGCTGTGG AGGCGCCATC 
CTGCCCAGCA TGCCTCGGTC CTACAAGCCT CAGAGGGTCG TGATCACATG TTCCCAGGAC 
TTCCCTCGAT GTGCCGTTCC ATATCGGGTT GGGCTGCCTG TCCTCTCACC CGAGTTCCTG 
CTGACGGGAG TACTGAAGCA GGAAGTCAAG CCAGAGGCCT TTGCCTTCTC CACTGTGGAA 
ATGTCATCCA CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984127.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839895.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

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CloneID	OBa229829
Clone ID Related Accession (Same CDS sequence)	XM_024984127.1 , XM_024984126.1
Accession Version	XM_024984127.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTGACT TGAATTTCGA GATGCCACCT GCTGAGAAGG CTTCCAGGGG TGATCAGGAA 
TCCCCAGATG CTTGTCTGCC TCCTGCAGCG CCTGAAGCCT CAGCCGCACT CCCAAACTCC 
CTCATCTCTC AGATGCAAAA ACATCCCGCA CCTCAGTCCC TCCTTTTTCC CTCTCCAGCT 
CCTTTAGAAC TGCCTATTCC CAGGACCAGA CGAAATGAGA GTCAGGAAGC TCCAGAGACT 
CCCTTCTCCT CAGAGCTGAA CTCTGTCCAC CCAGAACCCA AAGTCAGGCC CCAGGGGTCC 
TCTCCAGTTT CTTCTCTACC CCTTGAGCCC CACCCTGCCA CCCCCACAGG CCAGCCTATT 
GCCCTTGAGC CCACATCTGG GGTCTCTGGG AGCGGGACAC ATAGTTCCTT TGATGTAACT 
GCCTCATCAG TTGTCCCCAC AGCCCTTGCA CTGCAGCCAT CCACCTCCAC AGACCAGCCT 
GTCACCCCTA AGCCCACACT TGGGGCCCCT CGGGGCAGGG CACAGAGGTC TTCTGTCAAA 
ACCCCTGAAC CCAGTGTCCG CATAGACCAG CCTATTGCCC CTGAGCTCAC AGCTAAGGCC 
ACTCGGGGCA GGGCACAGAG GTCTTCTGTC AAGACTCCCA AACCAGATAA CTCCACAACA 
CCCAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAGCC CACATCTCAG 
GCCCCTCGGG GCAGGACACC TAAGTCTTCT GCCAAGACTC CTGAACCAGC TGTCCCCACA 
GCCTCTGAGC TCCAGCCTGC TGCCCCTAAA GACCAGCCTG TTGCTCCTGA GCTCACATCT 
AGAGCCACTC GGGGCAGGAC ACAGAGGTCT TCTATCAAGA CTTCCAAACC AGATACGTCC 
ACAGCCCCGA AGCCCCAGCC TTCCACTTCC ACAGACCAGC CTGTCACCCC CAAACCCACG 
TCTCGGGCCT CTCGAGGCAG GACACCTAGG TCTTCCACCA AGACTCCTGA ACCAGTTGTC 
TCCACAGCCT CTGAGCTCCA GCCTTCTGCC CTCACAGACC AGCCTATCAC TCCTGAGCTC 
ACATCTAGGG CTACTCGGGG CAGGGCACAG AGATCCTCTG TCAAAACCCC TGATCCAGTT 
ACCACCACAA CACCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCTTGT CACCCCCAAA 
CGCCCATCTC GGGCCCCTCG AGGCAGGACA CGTAGGTCGT CTGCCAAGAC TCCTGAACCA 
GTTGTTCCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCCTG CAGACCAGCC TGTTGGTCCT 
TGGGCCACTC AGTGTAGAAG ACATAGGTCT TCTGTCAAGA CCCCGGAACC AGTTGTCCCC 
ACAGTCCCTG AACCTCAGCC TTCTACTTCT AAAGACCAGT CTGTCGCTCC TGAACCCACA 
TCTCAGGCCA CTCAGAGCCA AACACATAGG TCTTCTGTCA AGACATCCCA GCCAACTGAA 
CCCACAGCCC CTGACCTCAA ACCTTCCTCC CCCACAGACC AGCCTGTCAC TCCCAAGGTC 
ATAGCTCAGG GTGGTCCAAG CAGGACACGA AGGGCTTCTA CAGCAAGTGC TGTGCTGGTT 
CCTACTACCC CTGAATTCCA GTCTCCAGTC CGCTCAGAAC AGCCTCTTTC CCCTGACCCC 
ATCCCCGAAG TCAACTGCAG CCTGAGGCCG AGGGCCACTA GGAGACATGG GTCTCCCACA 
GCTCATGTTC ATGAGCCCTG CACCACACCC CCTGAACCTA ACTCTCGCTC CTCAAGGAAC 
CAAACACATG GGGCAATGAA GGCAGCCAAG CCCCTTAGCA CCATTTCTGA GCCTGCCTTT 
GCCCAGCTCC CTGAGGCACC GCCTCACACT CCCCAGATGC CAAAGGAGGA GGCAGCTGAT 
GGGTCAGGCT TCACCCCAGA GCCCCAGCCT AGGGCCTCTC AAAACCGCAA GAGGCCTTCA 
GCTACTGCAC ATTCACCTCC ACTTCAAAAA CGGCTCCAGA GAGGGAGAGT CCCTCAGAAG 
GCAGCATCCC TTAAGGAAGA AGAAAATCCA GCAGTAAGGC CGAGGAAGGA AGAGGGTGTA 
GTGATTCCAG GTCCAGGCAA GAGAAGGAGA GAGCAGACAG AGGAAGAGTC CCAGGGAAGA 
CCGAGCCGTA GCCTGCGACG GACCAAACCT GTCCAGGAGT CCACGGCCCC CAAAGTGCTC 
TTCACGGGCG TGGTGGATGC TCGCGGAGAG AGGACGGTGC TGGCCCTGGG GGGCAGTCTG 
GCCAGCTCAG TGGCTGAGGC TTCTCACCTG GTGACTGATC GGATCCGCCG TACGGTCAAG 
TTTCTGTGTG CCCTGGGCCG GGGCATCCCC ATCCTCTCCC TGGCCTGGCT GCATGAGTCC 
CGCAAGGCAG GCTGCTTCTT GCCCCCGGAC GAATATTTGG TGACTGATCC TGAGCAGGAG 
AAGAACTTCG GCTTCAGCCT TCGGGAGGCC CTGAGCCGAG CTCGGGAGCG AAGGCTGCTG 
GAGGGCTATG AGATTCACGT GACCCCCGGA GTCCAGCCAC CGCCACCTCA GATGGGAGAA 
ATCATCAACT GCTGTGGAGG CGCCATCCTG CCCAGCATGC CTCGGTCCTA CAAGCCTCAG 
AGGGTCGTGA TCACATGTTC CCAGGACTTC CCTCGATGTG CCGTTCCATA TCGGGTTGGG 
CTGCCTGTCC TCTCACCCGA GTTCCTGCTG ACGGGAGTAC TGAAGCAGGA AGTCAAGCCA 
GAGGCCTTTG CCTTCTCCAC TGTGGAAATG TCATCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839895.1
CDS	133..5334
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984127.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984127.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTGACT TGAATTTCGA GATGCCACCT GCTGAGAAGG CTTCCAGGGG TGATCAGGAA 
TCCCCAGATG CTTGTCTGCC TCCTGCAGCG CCTGAAGCCT CAGCCGCACT CCCAAACTCC 
CTCATCTCTC AGATGCAAAA ACATCCCGCA CCTCAGTCCC TCCTTTTTCC CTCTCCAGCT 
CCTTTAGAAC TGCCTATTCC CAGGACCAGA CGAAATGAGA GTCAGGAAGC TCCAGAGACT 
CCCTTCTCCT CAGAGCTGAA CTCTGTCCAC CCAGAACCCA AAGTCAGGCC CCAGGGGTCC 
TCTCCAGTTT CTTCTCTACC CCTTGAGCCC CACCCTGCCA CCCCCACAGG CCAGCCTATT 
GCCCTTGAGC CCACATCTGG GGTCTCTGGG AGCGGGACAC ATAGTTCCTT TGATGTAACT 
GCCTCATCAG TTGTCCCCAC AGCCCTTGCA CTGCAGCCAT CCACCTCCAC AGACCAGCCT 
GTCACCCCTA AGCCCACACT TGGGGCCCCT CGGGGCAGGG CACAGAGGTC TTCTGTCAAA 
ACCCCTGAAC CCAGTGTCCG CATAGACCAG CCTATTGCCC CTGAGCTCAC AGCTAAGGCC 
ACTCGGGGCA GGGCACAGAG GTCTTCTGTC AAGACTCCCA AACCAGATAA CTCCACAACA 
CCCAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAGCC CACATCTCAG 
GCCCCTCGGG GCAGGACACC TAAGTCTTCT GCCAAGACTC CTGAACCAGC TGTCCCCACA 
GCCTCTGAGC TCCAGCCTGC TGCCCCTAAA GACCAGCCTG TTGCTCCTGA GCTCACATCT 
AGAGCCACTC GGGGCAGGAC ACAGAGGTCT TCTATCAAGA CTTCCAAACC AGATACGTCC 
ACAGCCCCGA AGCCCCAGCC TTCCACTTCC ACAGACCAGC CTGTCACCCC CAAACCCACG 
TCTCGGGCCT CTCGAGGCAG GACACCTAGG TCTTCCACCA AGACTCCTGA ACCAGTTGTC 
TCCACAGCCT CTGAGCTCCA GCCTTCTGCC CTCACAGACC AGCCTATCAC TCCTGAGCTC 
ACATCTAGGG CTACTCGGGG CAGGGCACAG AGATCCTCTG TCAAAACCCC TGATCCAGTT 
ACCACCACAA CACCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCTTGT CACCCCCAAA 
CGCCCATCTC GGGCCCCTCG AGGCAGGACA CGTAGGTCGT CTGCCAAGAC TCCTGAACCA 
GTTGTTCCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCCTG CAGACCAGCC TGTTGGTCCT 
TGGGCCACTC AGTGTAGAAG ACATAGGTCT TCTGTCAAGA CCCCGGAACC AGTTGTCCCC 
ACAGTCCCTG AACCTCAGCC TTCTACTTCT AAAGACCAGT CTGTCGCTCC TGAACCCACA 
TCTCAGGCCA CTCAGAGCCA AACACATAGG TCTTCTGTCA AGACATCCCA GCCAACTGAA 
CCCACAGCCC CTGACCTCAA ACCTTCCTCC CCCACAGACC AGCCTGTCAC TCCCAAGGTC 
ATAGCTCAGG GTGGTCCAAG CAGGACACGA AGGGCTTCTA CAGCAAGTGC TGTGCTGGTT 
CCTACTACCC CTGAATTCCA GTCTCCAGTC CGCTCAGAAC AGCCTCTTTC CCCTGACCCC 
ATCCCCGAAG TCAACTGCAG CCTGAGGCCG AGGGCCACTA GGAGACATGG GTCTCCCACA 
GCTCATGTTC ATGAGCCCTG CACCACACCC CCTGAACCTA ACTCTCGCTC CTCAAGGAAC 
CAAACACATG GGGCAATGAA GGCAGCCAAG CCCCTTAGCA CCATTTCTGA GCCTGCCTTT 
GCCCAGCTCC CTGAGGCACC GCCTCACACT CCCCAGATGC CAAAGGAGGA GGCAGCTGAT 
GGGTCAGGCT TCACCCCAGA GCCCCAGCCT AGGGCCTCTC AAAACCGCAA GAGGCCTTCA 
GCTACTGCAC ATTCACCTCC ACTTCAAAAA CGGCTCCAGA GAGGGAGAGT CCCTCAGAAG 
GCAGCATCCC TTAAGGAAGA AGAAAATCCA GCAGTAAGGC CGAGGAAGGA AGAGGGTGTA 
GTGATTCCAG GTCCAGGCAA GAGAAGGAGA GAGCAGACAG AGGAAGAGTC CCAGGGAAGA 
CCGAGCCGTA GCCTGCGACG GACCAAACCT GTCCAGGAGT CCACGGCCCC CAAAGTGCTC 
TTCACGGGCG TGGTGGATGC TCGCGGAGAG AGGACGGTGC TGGCCCTGGG GGGCAGTCTG 
GCCAGCTCAG TGGCTGAGGC TTCTCACCTG GTGACTGATC GGATCCGCCG TACGGTCAAG 
TTTCTGTGTG CCCTGGGCCG GGGCATCCCC ATCCTCTCCC TGGCCTGGCT GCATGAGTCC 
CGCAAGGCAG GCTGCTTCTT GCCCCCGGAC GAATATTTGG TGACTGATCC TGAGCAGGAG 
AAGAACTTCG GCTTCAGCCT TCGGGAGGCC CTGAGCCGAG CTCGGGAGCG AAGGCTGCTG 
GAGGGCTATG AGATTCACGT GACCCCCGGA GTCCAGCCAC CGCCACCTCA GATGGGAGAA 
ATCATCAACT GCTGTGGAGG CGCCATCCTG CCCAGCATGC CTCGGTCCTA CAAGCCTCAG 
AGGGTCGTGA TCACATGTTC CCAGGACTTC CCTCGATGTG CCGTTCCATA TCGGGTTGGG 
CTGCCTGTCC TCTCACCCGA GTTCCTGCTG ACGGGAGTAC TGAAGCAGGA AGTCAAGCCA 
GAGGCCTTTG CCTTCTCCAC TGTGGAAATG TCATCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984126.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839894.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

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CloneID	OBa229829
Clone ID Related Accession (Same CDS sequence)	XM_024984127.1 , XM_024984126.1
Accession Version	XM_024984126.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTGACT TGAATTTCGA GATGCCACCT GCTGAGAAGG CTTCCAGGGG TGATCAGGAA 
TCCCCAGATG CTTGTCTGCC TCCTGCAGCG CCTGAAGCCT CAGCCGCACT CCCAAACTCC 
CTCATCTCTC AGATGCAAAA ACATCCCGCA CCTCAGTCCC TCCTTTTTCC CTCTCCAGCT 
CCTTTAGAAC TGCCTATTCC CAGGACCAGA CGAAATGAGA GTCAGGAAGC TCCAGAGACT 
CCCTTCTCCT CAGAGCTGAA CTCTGTCCAC CCAGAACCCA AAGTCAGGCC CCAGGGGTCC 
TCTCCAGTTT CTTCTCTACC CCTTGAGCCC CACCCTGCCA CCCCCACAGG CCAGCCTATT 
GCCCTTGAGC CCACATCTGG GGTCTCTGGG AGCGGGACAC ATAGTTCCTT TGATGTAACT 
GCCTCATCAG TTGTCCCCAC AGCCCTTGCA CTGCAGCCAT CCACCTCCAC AGACCAGCCT 
GTCACCCCTA AGCCCACACT TGGGGCCCCT CGGGGCAGGG CACAGAGGTC TTCTGTCAAA 
ACCCCTGAAC CCAGTGTCCG CATAGACCAG CCTATTGCCC CTGAGCTCAC AGCTAAGGCC 
ACTCGGGGCA GGGCACAGAG GTCTTCTGTC AAGACTCCCA AACCAGATAA CTCCACAACA 
CCCAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAGCC CACATCTCAG 
GCCCCTCGGG GCAGGACACC TAAGTCTTCT GCCAAGACTC CTGAACCAGC TGTCCCCACA 
GCCTCTGAGC TCCAGCCTGC TGCCCCTAAA GACCAGCCTG TTGCTCCTGA GCTCACATCT 
AGAGCCACTC GGGGCAGGAC ACAGAGGTCT TCTATCAAGA CTTCCAAACC AGATACGTCC 
ACAGCCCCGA AGCCCCAGCC TTCCACTTCC ACAGACCAGC CTGTCACCCC CAAACCCACG 
TCTCGGGCCT CTCGAGGCAG GACACCTAGG TCTTCCACCA AGACTCCTGA ACCAGTTGTC 
TCCACAGCCT CTGAGCTCCA GCCTTCTGCC CTCACAGACC AGCCTATCAC TCCTGAGCTC 
ACATCTAGGG CTACTCGGGG CAGGGCACAG AGATCCTCTG TCAAAACCCC TGATCCAGTT 
ACCACCACAA CACCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCTTGT CACCCCCAAA 
CGCCCATCTC GGGCCCCTCG AGGCAGGACA CGTAGGTCGT CTGCCAAGAC TCCTGAACCA 
GTTGTTCCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCCTG CAGACCAGCC TGTTGGTCCT 
TGGGCCACTC AGTGTAGAAG ACATAGGTCT TCTGTCAAGA CCCCGGAACC AGTTGTCCCC 
ACAGTCCCTG AACCTCAGCC TTCTACTTCT AAAGACCAGT CTGTCGCTCC TGAACCCACA 
TCTCAGGCCA CTCAGAGCCA AACACATAGG TCTTCTGTCA AGACATCCCA GCCAACTGAA 
CCCACAGCCC CTGACCTCAA ACCTTCCTCC CCCACAGACC AGCCTGTCAC TCCCAAGGTC 
ATAGCTCAGG GTGGTCCAAG CAGGACACGA AGGGCTTCTA CAGCAAGTGC TGTGCTGGTT 
CCTACTACCC CTGAATTCCA GTCTCCAGTC CGCTCAGAAC AGCCTCTTTC CCCTGACCCC 
ATCCCCGAAG TCAACTGCAG CCTGAGGCCG AGGGCCACTA GGAGACATGG GTCTCCCACA 
GCTCATGTTC ATGAGCCCTG CACCACACCC CCTGAACCTA ACTCTCGCTC CTCAAGGAAC 
CAAACACATG GGGCAATGAA GGCAGCCAAG CCCCTTAGCA CCATTTCTGA GCCTGCCTTT 
GCCCAGCTCC CTGAGGCACC GCCTCACACT CCCCAGATGC CAAAGGAGGA GGCAGCTGAT 
GGGTCAGGCT TCACCCCAGA GCCCCAGCCT AGGGCCTCTC AAAACCGCAA GAGGCCTTCA 
GCTACTGCAC ATTCACCTCC ACTTCAAAAA CGGCTCCAGA GAGGGAGAGT CCCTCAGAAG 
GCAGCATCCC TTAAGGAAGA AGAAAATCCA GCAGTAAGGC CGAGGAAGGA AGAGGGTGTA 
GTGATTCCAG GTCCAGGCAA GAGAAGGAGA GAGCAGACAG AGGAAGAGTC CCAGGGAAGA 
CCGAGCCGTA GCCTGCGACG GACCAAACCT GTCCAGGAGT CCACGGCCCC CAAAGTGCTC 
TTCACGGGCG TGGTGGATGC TCGCGGAGAG AGGACGGTGC TGGCCCTGGG GGGCAGTCTG 
GCCAGCTCAG TGGCTGAGGC TTCTCACCTG GTGACTGATC GGATCCGCCG TACGGTCAAG 
TTTCTGTGTG CCCTGGGCCG GGGCATCCCC ATCCTCTCCC TGGCCTGGCT GCATGAGTCC 
CGCAAGGCAG GCTGCTTCTT GCCCCCGGAC GAATATTTGG TGACTGATCC TGAGCAGGAG 
AAGAACTTCG GCTTCAGCCT TCGGGAGGCC CTGAGCCGAG CTCGGGAGCG AAGGCTGCTG 
GAGGGCTATG AGATTCACGT GACCCCCGGA GTCCAGCCAC CGCCACCTCA GATGGGAGAA 
ATCATCAACT GCTGTGGAGG CGCCATCCTG CCCAGCATGC CTCGGTCCTA CAAGCCTCAG 
AGGGTCGTGA TCACATGTTC CCAGGACTTC CCTCGATGTG CCGTTCCATA TCGGGTTGGG 
CTGCCTGTCC TCTCACCCGA GTTCCTGCTG ACGGGAGTAC TGAAGCAGGA AGTCAAGCCA 
GAGGCCTTTG CCTTCTCCAC TGTGGAAATG TCATCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839894.1
CDS	201..5402
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984126.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984126.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GGTAAGTGGA ACCGAGATCC AGATGCAGAA GAGGACAGGG CCCAACCAGT GGCCCTTCTG 
GAGCGAAGCC AGGCCTCTGC TAGGGGAGAC AGTGACACAG ATGTGGAGGA AGAGGGGCTC 
CCAGTGGGAA GGAGGGGAAT GGTTCCCAAG GGTCACATGG ACAGGGAATA TTCAAAAAAG 
AGCCAGCATC CTCCCAGGGA CAGTGATACA GAGGGGAAGA AAGATGAGAG CTCTCCGGGG 
GTCCACCTGG AGAGAAGCCA GGCCTCTGCA CAAGTGGAGG ACGAGGTCCC ACTGGGGCCA 
GCTGTTGCAC TTCCGGAGAA ACATCAGGTA CAAGGCATAG TGTGGACACA TCACACCGAT 
GCGGAGGCAG AAGGGGGCCC GGCACAGCTC CCCGTGCTGC GTCTAGAGGA AGCCCGGCCT 
CCTCTAGCTG GGGACTGTGA GCTGGATGCG GAGAACACAT CCTCAGCAGC AGCTGGTGTC 
AGAAAGAGCC AGCTTCCGGC GGAAAAAGAT GCTGGGACCA CGTGGGCCGC AGCCATTCCT 
GAACAGGACA GAACACTTGC CACCAGGACC CAGGGTGGGT CATCCACGGC ACCAGGGGAG 
CAGGACCTTC TCCCGGTCTC AAGGGAAAAC CTAGCAAATC CGGTGGTGGA TACAGGCACT 
CCACGGGAGC CCCAGCCACA GAGAGAGGGG GCCCAGACCA CCACAGGAAG GGAGAAAGAA 
CCACATGGGA ATAGGGCCAC AGACTCTGGA GAGAGCCTCC ATGATTCTGA AGATCTGGAC 
CTACCAGCTA CCCAGTGCTT TGCAGACAGA GAGAATCAGA GCCTGGAAGC AGCCCCCAGC 
ATGGAGGATG AGCCCACCCA GGCCTTCCTG TTTACTCTGC CCCAAGAGCC TGGCCCTTCC 
TGTTGCAGCT CCCAGGCCAC AGGTTCCCTG AATGAGGCAT GGGAGGTCTT GGCGACACAG 
CCATTCTGTC CGAGAGAGTA TGAGGCCTCC GAGACCCAAA CCGCTGTCAC CCTCCTTGAC 
ACCCGTGCAT CTTGCCCCCC TCCATCTAGG ACAGCACAGC AAGAGCAACA TTCAGAGAGC 
CCAGTCCATG CAGAAGCACT GGGGATGGAA GGCAGAGGGA TGCAGACCGT GGAGAAAGAC 
ATGGGTGACT TGAATTTCGA GATGCCACCT GCTGAGAAGG CTTCCAGGGG TGATCAGGAA 
TCCCCAGATG CTTGTCTGCC TCCTGCAGCG CCTGAAGCCT CAGCCGCACT CCCAAACTCC 
CTCATCTCTC AGATGCAAAA ACATCCCGCA CCTCAGTCCC TCCTTTTTCC CTCTCCAGCT 
CCTTTAGAAC TGCCTATTCC CAGGACCAGA CGAAATGAGA GTCAGGAAGC TCCAGAGACT 
CCCTTCTCCT CAGAGCTGAA CTCTGTCCAC CCAGAACCCA AAGTCAGGCC CCAGGGGTCC 
TCTCCAGTTT CTTCTCTACC CCTTGAGCCC CACCCTGCCA CCCCCACAGG CCAGCCTATT 
GCCCTTGAGC CCACATCTGG GGTCTCTGGG AGCGGGACAC ATAGTTCCTT TGATGTAACT 
GCCTCATCAG TTGTCCCCAC AGCCCTTGCA CTGCAGCCAT CCACCTCCAC AGACCAGCCT 
GTCACCCCTA AGCCCACACT TGGGGCCCCT CGGGGCAGGG CACAGAGGTC TTCTGTCAAA 
ACCCCTGAAC CCAGTGTCCG CATAGACCAG CCTATTGCCC CTGAGCTCAC AGCTAAGGCC 
ACTCGGGGCA GGGCACAGAG GTCTTCTGTC AAGACTCCCA AACCAGATAA CTCCACAACA 
CCCAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAGCC CACATCTCAG 
GCCCCTCGGG GCAGGACACC TAAGTCTTCT GCCAAGACTC CTGAACCAGC TGTCCCCACA 
GCCTCTGAGC TCCAGCCTGC TGCCCCTAAA GACCAGCCTG TTGCTCCTGA GCTCACATCT 
AGAGCCACTC GGGGCAGGAC ACAGAGGTCT TCTATCAAGA CTTCCAAACC AGATACGTCC 
ACAGCCCCGA AGCCCCAGCC TTCCACTTCC ACAGACCAGC CTGTCACCCC CAAACCCACG 
TCTCGGGCCT CTCGAGGCAG GACACCTAGG TCTTCCACCA AGACTCCTGA ACCAGTTGTC 
TCCACAGCCT CTGAGCTCCA GCCTTCTGCC CTCACAGACC AGCCTATCAC TCCTGAGCTC 
ACATCTAGGG CTACTCGGGG CAGGGCACAG AGATCCTCTG TCAAAACCCC TGATCCAGTT 
ACCACCACAA CACCTGAGCT CCAGCCTTCC ACCTCCACAG ACCAGCTTGT CACCCCCAAA 
CGCCCATCTC GGGCCCCTCG AGGCAGGACA CGTAGGTCGT CTGCCAAGAC TCCTGAACCA 
GTTGTTCCCA CAGCCTCTGA GCTCCAGCCT TCTGCCCCTG CAGACCAGCC TGTTGGTCCT 
TGGGCCACTC AGTGTAGAAG ACATAGGTCT TCTGTCAAGA CCCCGGAACC AGTTGTCCCC 
ACAGTCCCTG AACCTCAGCC TTCTACTTCT AAAGACCAGT CTGTCGCTCC TGAACCCACA 
TCTCAGGCCA CTCAGAGCCA AACACATAGG TCTTCTGTCA AGACATCCCA GCCAACTGAA 
CCCACAGCCC CTGACCTCAA ACCTTCCTCC CCCACAGACC AGCCTGTCAC TCCCAAGGTC 
ATAGCTCAGG GTGGTCCAAG CAGGACACGA AGGGCTTCTA CAGCAAGTGC TGTGCTGGTT 
CCTACTACCC CTGAATTCCA GTCTCCAGTC CGCTCAGAAC AGCCTCTTTC CCCTGACCCC 
ATCCCCGAAG TCAACTGCAG CCTGAGGCCG AGGGCCACTA GGAGACATGG GTCTCCCACA 
GCTCATGTTC ATGAGCCCTG CACCACACCC CCTGAACCTA ACTCTCGCTC CTCAAGGAAC 
CAAACACATG GGGCAATGAA GGCAGCCAAG CCCCTTAGCA CCATTTCTGA GCCTGCCTTT 
GCCCAGCTCC CTGAGGCACC GCCTCACACT CCCCAGATGC CAAAGGAGGA GGCAGCTGAT 
GGGTCAGGCT TCACCCCAGA GCCCCAGCCT AGGGCCTCTC AAAACCGCAA GAGGCCTTCA 
GCTACTGCAC ATTCACCTCC ACTTCAAAAA CGGCTCCAGA GAGGGAGAGT CCCTCAGAAG 
GCAGCATCCC TTAAGGAAGA AGAAAATCCA GCAGTAAGGC CGAGGAAGGA AGAGGGTGTA 
GTGATTCCAG GTCCAGGCAA GAGAAGGAGA GAGCAGACAG AGGAAGAGTC CCAGGGAAGA 
CCGAGCCGTA GCCTGCGACG GACCAAACCT GTCCAGGAGT CCACGGCCCC CAAAGTGCTC 
TTCACGGGCG TGGTGGATGC TCGCGGAGAG AGGACGGTGC TGGCCCTGGG GGGCAGTCTG 
GCCAGCTCAG TGGCTGAGGC TTCTCACCTG GTGACTGATC GGATCCGCCG TACGGTCAAG 
TTTCTGTGTG CCCTGGGCCG GGGCATCCCC ATCCTCTCCC TGGCCTGGCT GCATGAGTCC 
CGCAAGGCAG GCTGCTTCTT GCCCCCGGAC GAATATTTGG TGACTGATCC TGAGCAGGAG 
AAGAACTTCG GCTTCAGCCT TCGGGAGGCC CTGAGCCGAG CTCGGGAGCG AAGGCTGCTG 
GAGGGCTATG AGATTCACGT GACCCCCGGA GTCCAGCCAC CGCCACCTCA GATGGGAGAA 
ATCATCAACT GCTGTGGAGG CGCCATCCTG CCCAGCATGC CTCGGTCCTA CAAGCCTCAG 
AGGGTCGTGA TCACATGTTC CCAGGACTTC CCTCGATGTG CCGTTCCATA TCGGGTTGGG 
CTGCCTGTCC TCTCACCCGA GTTCCTGCTG ACGGGAGTAC TGAAGCAGGA AGTCAAGCCA 
GAGGCCTTTG CCTTCTCCAC TGTGGAAATG TCATCCACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984129.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839897.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

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CloneID	OBa229830
Clone ID Related Accession (Same CDS sequence)	XM_024984129.1 , XM_024984128.1 , XM_024984130.1
Accession Version	XM_024984129.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839897.1
CDS	344..5359
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQADSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984129.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984129.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984128.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839896.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

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CloneID	OBa229830
Clone ID Related Accession (Same CDS sequence)	XM_024984129.1 , XM_024984128.1 , XM_024984130.1
Accession Version	XM_024984128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839896.1
CDS	199..5214
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQADSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984128.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984128.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984130.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839898.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

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CloneID	OBa229830
Clone ID Related Accession (Same CDS sequence)	XM_024984129.1 , XM_024984128.1 , XM_024984130.1
Accession Version	XM_024984130.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5016bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839898.1
CDS	160..5175
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQADSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984130.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984130.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCAG CCCCCAGCAT GGAGGATGAG CCCACCCAGG CCTTCCTGTT TACTCTGCCC 
CAAGAGCCTG GCCCTTCCTG TTGCAGCTCC CAGGCCACAG GTTCCCTGAA TGAGGCATGG 
GAGGTCTTGG CGACACAGCC ATTCTGTCCG AGAGAGTATG AGGCCTCCGA GACCCAAACC 
GCTGTCACCC TCCTTGACAC CCGTGCATCT TGCCCCCCTC CATCTAGGAC AGCACAGCAA 
GAGCAACATT CAGAGAGCCC AGTCCATGCA GAAGCACTGG GGATGGAAGG CAGAGGGATG 
CAGACCGTGG AGAAAGACAT GGGTACCCAA AGAGAAACAG CAGAGAGGGT GATCCCTGAG 
GGAGGGCCAC CGCAGAATGA AACCAAGAAA CTGCCCTCGG AAGGAGAGAG GGAAGATGTG 
ACGGGAGAGG AAGAATTAAT CGGGGGGATA CAGGGTAGAG AACAAAATCA GGTGTTAGCT 
AGAGATACTC AGAGCCAAGA ATCTGACAAA ACAGTGAAAA GTGCAAGTAC TGGAAGGGGA 
ATGGAGATTG TAAAGTTAGA AACTGAGACA CCCAAGGAAA CACAGGAGAA AGAGAGAGAA 
AAGCAGACTC TCGCAGGGGA AATATTTGAG AGTGAAGCAG GGAAACTGGT AGCAGAGAGA 
GAGAGTGAGG TAGGTGGGTT AGAAGTCAAG GGACCCCAAG AGCTACTGGA CAGAGGCCCA 
CAGATGAGGG AGACAGAGGC GGGGGGCCAG GACCAGAAAG GCCAAGCCTC TGGTCCGCCA 
TCAGAGCCTG GAGCAGGGGC AGGAGACCTT CAGGGATTTA CTTCAGATCC AGTAGCTTCT 
GGGAGCCAGG CAGGTGGAGG AAGGGGAGCC CCAGGGAGCC CCAGGAGGCA GCAGAGAGGT 
GACTTGAATT TCGAGATGCC ACCTGCTGAG AAGGCTTCCA GGGGTGATCA GGAATCCCCA 
GATGCTTGTC TGCCTCCTGC AGCGCCTGAA GCCTCAGCCG CACTCCCAAA CTCCCTCATC 
TCTCAGATGC AAAAACATCC CGCACCTCAG TCCCTCCTTT TTCCCTCTCC AGCTCCTTTA 
GAACTGCCTA TTCCCAGGAC CAGACGAAAT GAGAGTCAGG AAGCTCCAGA GACTCCCTTC 
TCCTCAGAGC TGAACTCTGT CCACCCAGAA CCCAAAGTCA GGCCCCAGGG GTCCTCTCCA 
GTTTCTTCTC TACCCCTTGA GCCCCACCCT GCCACCCCCA CAGGCCAGCC TATTGCCCTT 
GAGCCCACAT CTGGGGTCTC TGGGAGCGGG ACACATAGTT CCTTTGATGT AACTGCCTCA 
TCAGTTGTCC CCACAGCCCT TGCACTGCAG CCATCCACCT CCACAGACCA GCCTGTCACC 
CCTAAGCCCA CACTTGGGGC CCCTCGGGGC AGGGCACAGA GGTCTTCTGT CAAAACCCCT 
GAACCCAGTG TCCGCATAGA CCAGCCTATT GCCCCTGAGC TCACAGCTAA GGCCACTCGG 
GGCAGGGCAC AGAGGTCTTC TGTCAAGACT CCCAAACCAG ATAACTCCAC AACACCCAAG 
CCCCAGCCTT CCACTTCCAC AGACCAGCCT GTCACCCCCA AGCCCACATC TCAGGCCCCT 
CGGGGCAGGA CACCTAAGTC TTCTGCCAAG ACTCCTGAAC CAGCTGTCCC CACAGCCTCT 
GAGCTCCAGC CTGCTGCCCC TAAAGACCAG CCTGTTGCTC CTGAGCTCAC ATCTAGAGCC 
ACTCGGGGCA GGACACAGAG GTCTTCTATC AAGACTTCCA AACCAGATAC GTCCACAGCC 
CCGAAGCCCC AGCCTTCCAC TTCCACAGAC CAGCCTGTCA CCCCCAAACC CACGTCTCGG 
GCCTCTCGAG GCAGGACACC TAGGTCTTCC ACCAAGACTC CTGAACCAGT TGTCTCCACA 
GCCTCTGAGC TCCAGCCTTC TGCCCTCACA GACCAGCCTA TCACTCCTGA GCTCACATCT 
AGGGCTACTC GGGGCAGGGC ACAGAGATCC TCTGTCAAAA CCCCTGATCC AGTTACCACC 
ACAACACCTG AGCTCCAGCC TTCCACCTCC ACAGACCAGC TTGTCACCCC CAAACGCCCA 
TCTCGGGCCC CTCGAGGCAG GACACGTAGG TCGTCTGCCA AGACTCCTGA ACCAGTTGTT 
CCCACAGCCT CTGAGCTCCA GCCTTCTGCC CCTGCAGACC AGCCTGTTGG TCCTTGGGCC 
ACTCAGTGTA GAAGACATAG GTCTTCTGTC AAGACCCCGG AACCAGTTGT CCCCACAGTC 
CCTGAACCTC AGCCTTCTAC TTCTAAAGAC CAGTCTGTCG CTCCTGAACC CACATCTCAG 
GCCACTCAGA GCCAAACACA TAGGTCTTCT GTCAAGACAT CCCAGCCAAC TGAACCCACA 
GCCCCTGACC TCAAACCTTC CTCCCCCACA GACCAGCCTG TCACTCCCAA GGTCATAGCT 
CAGGGTGGTC CAAGCAGGAC ACGAAGGGCT TCTACAGCAA GTGCTGTGCT GGTTCCTACT 
ACCCCTGAAT TCCAGTCTCC AGTCCGCTCA GAACAGCCTC TTTCCCCTGA CCCCATCCCC 
GAAGTCAACT GCAGCCTGAG GCCGAGGGCC ACTAGGAGAC ATGGGTCTCC CACAGCTCAT 
GTTCATGAGC CCTGCACCAC ACCCCCTGAA CCTAACTCTC GCTCCTCAAG GAACCAAACA 
CATGGGGCAA TGAAGGCAGC CAAGCCCCTT AGCACCATTT CTGAGCCTGC CTTTGCCCAG 
CTCCCTGAGG CACCGCCTCA CACTCCCCAG ATGCCAAAGG AGGAGGCAGC TGATGGGTCA 
GGCTTCACCC CAGAGCCCCA GCCTAGGGCC TCTCAAAACC GCAAGAGGCC TTCAGCTACT 
GCACATTCAC CTCCACTTCA AAAACGGCTC CAGAGAGGGA GAGTCCCTCA GAAGGCAGCA 
TCCCTTAAGG AAGAAGAAAA TCCAGCAGTA AGGCCGAGGA AGGAAGAGGG TGTAGTGATT 
CCAGGTCCAG GCAAGAGAAG GAGAGAGCAG ACAGAGGAAG AGTCCCAGGG AAGACCGAGC 
CGTAGCCTGC GACGGACCAA ACCTGTCCAG GAGTCCACGG CCCCCAAAGT GCTCTTCACG 
GGCGTGGTGG ATGCTCGCGG AGAGAGGACG GTGCTGGCCC TGGGGGGCAG TCTGGCCAGC 
TCAGTGGCTG AGGCTTCTCA CCTGGTGACT GATCGGATCC GCCGTACGGT CAAGTTTCTG 
TGTGCCCTGG GCCGGGGCAT CCCCATCCTC TCCCTGGCCT GGCTGCATGA GTCCCGCAAG 
GCAGGCTGCT TCTTGCCCCC GGACGAATAT TTGGTGACTG ATCCTGAGCA GGAGAAGAAC 
TTCGGCTTCA GCCTTCGGGA GGCCCTGAGC CGAGCTCGGG AGCGAAGGCT GCTGGAGGGC 
TATGAGATTC ACGTGACCCC CGGAGTCCAG CCACCGCCAC CTCAGATGGG AGAAATCATC 
AACTGCTGTG GAGGCGCCAT CCTGCCCAGC ATGCCTCGGT CCTACAAGCC TCAGAGGGTC 
GTGATCACAT GTTCCCAGGA CTTCCCTCGA TGTGCCGTTC CATATCGGGT TGGGCTGCCT 
GTCCTCTCAC CCGAGTTCCT GCTGACGGGA GTACTGAAGC AGGAAGTCAA GCCAGAGGCC 
TTTGCCTTCT CCACTGTGGA AATGTCATCC ACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_024984131.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024839899.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBa229831
Clone ID Related Accession (Same CDS sequence)	XM_024984131.1
Accession Version	XM_024984131.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037350.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCCC CCAGCATGGA GGATGAGCCC ACCCAGGCCT TCCTGTTTAC TCTGCCCCAA 
GAGCCTGGCC CTTCCTGTTG CAGCTCCCAG GCCACAGGTT CCCTGAATGA GGCATGGGAG 
GTCTTGGCGA CACAGCCATT CTGTCCGAGA GAGTATGAGG CCTCCGAGAC CCAAACCGCT 
GTCACCCTCC TTGACACCCG TGCATCTTGC CCCCCTCCAT CTAGGACAGC ACAGCAAGAG 
CAACATTCAG AGAGCCCAGT CCATGCAGAA GCACTGGGGA TGGAAGGCAG AGGGATGCAG 
ACCGTGGAGA AAGACATGGG TACCCAAAGA GAAACAGCAG AGAGGGTGAT CCCTGAGGGA 
GGGCCACCGC AGAATGAAAC CAAGAAACTG CCCTCGGAAG GAGAGAGGGA AGATGTGACG 
GGAGAGGAAG AATTAATCGG GGGGATACAG GGTAGAGAAC AAAATCAGGT GTTAGCTAGA 
GATACTCAGA GCCAAGAATC TGACAAAACA GTGAAAAGTG CAAGTACTGG AAGGGGAATG 
GAGATTGTAA AGTTAGAAAC TGAGACACCC AAGGAAACAC AGGAGAAAGA GAGAGAAAAG 
CAGACTCTCG CAGGGGAAAT ATTTGAGAGT GAAGCAGGGA AACTGGTAGC AGAGAGAGAG 
AGTGAGGTAG GTGGGTTAGA AGTCAAGGGA CCCCAAGAGC TACTGGACAG AGGCCCACAG 
ATGAGGGAGA CAGAGGCGGG GGGCCAGGAC CAGAAAGGCC AAGCCTCTGG TCCGCCATCA 
GAGCCTGGAG CAGGGGCAGG AGACCTTCAG GGATTTACTT CAGATCCAGT AGCTTCTGGG 
AGCCAGGCAG GTGGAGGAAG GGGAGCCCCA GGGAGCCCCA GGAGGCAGCA GAGAGGTGAC 
TTGAATTTCG AGATGCCACC TGCTGAGAAG GCTTCCAGGG GTGATCAGGA ATCCCCAGAT 
GCTTGTCTGC CTCCTGCAGC GCCTGAAGCC TCAGCCGCAC TCCCAAACTC CCTCATCTCT 
CAGATGCAAA AACATCCCGC ACCTCAGTCC CTCCTTTTTC CCTCTCCAGC TCCTTTAGAA 
CTGCCTATTC CCAGGACCAG ACGAAATGAG AGTCAGGAAG CTCCAGAGAC TCCCTTCTCC 
TCAGAGCTGA ACTCTGTCCA CCCAGAACCC AAAGTCAGGC CCCAGGGGTC CTCTCCAGTT 
TCTTCTCTAC CCCTTGAGCC CCACCCTGCC ACCCCCACAG GCCAGCCTAT TGCCCTTGAG 
CCCACATCTG GGGTCTCTGG GAGCGGGACA CATAGTTCCT TTGATGTAAC TGCCTCATCA 
GTTGTCCCCA CAGCCCTTGC ACTGCAGCCA TCCACCTCCA CAGACCAGCC TGTCACCCCT 
AAGCCCACAC TTGGGGCCCC TCGGGGCAGG GCACAGAGGT CTTCTGTCAA AACCCCTGAA 
CCCAGTGTCC GCATAGACCA GCCTATTGCC CCTGAGCTCA CAGCTAAGGC CACTCGGGGC 
AGGGCACAGA GGTCTTCTGT CAAGACTCCC AAACCAGATA ACTCCACAAC ACCCAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAGC CCACATCTCA GGCCCCTCGG 
GGCAGGACAC CTAAGTCTTC TGCCAAGACT CCTGAACCAG CTGTCCCCAC AGCCTCTGAG 
CTCCAGCCTG CTGCCCCTAA AGACCAGCCT GTTGCTCCTG AGCTCACATC TAGAGCCACT 
CGGGGCAGGA CACAGAGGTC TTCTATCAAG ACTTCCAAAC CAGATACGTC CACAGCCCCG 
AAGCCCCAGC CTTCCACTTC CACAGACCAG CCTGTCACCC CCAAACCCAC GTCTCGGGCC 
TCTCGAGGCA GGACACCTAG GTCTTCCACC AAGACTCCTG AACCAGTTGT CTCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCTCACAGAC CAGCCTATCA CTCCTGAGCT CACATCTAGG 
GCTACTCGGG GCAGGGCACA GAGATCCTCT GTCAAAACCC CTGATCCAGT TACCACCACA 
ACACCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCTTG TCACCCCCAA ACGCCCATCT 
CGGGCCCCTC GAGGCAGGAC ACGTAGGTCG TCTGCCAAGA CTCCTGAACC AGTTGTTCCC 
ACAGCCTCTG AGCTCCAGCC TTCTGCCCCT GCAGACCAGC CTGTTGGTCC TTGGGCCACT 
CAGTGTAGAA GACATAGGTC TTCTGTCAAG ACCCCGGAAC CAGTTGTCCC CACAGTCCCT 
GAACCTCAGC CTTCTACTTC TAAAGACCAG TCTGTCGCTC CTGAACCCAC ATCTCAGGCC 
ACTCAGAGCC AAACACATAG GTCTTCTGTC AAGACATCCC AGCCAACTGA ACCCACAGCC 
CCTGACCTCA AACCTTCCTC CCCCACAGAC CAGCCTGTCA CTCCCAAGGT CATAGCTCAG 
GGTGGTCCAA GCAGGACACG AAGGGCTTCT ACAGCAAGTG CTGTGCTGGT TCCTACTACC 
CCTGAATTCC AGTCTCCAGT CCGCTCAGAA CAGCCTCTTT CCCCTGACCC CATCCCCGAA 
GTCAACTGCA GCCTGAGGCC GAGGGCCACT AGGAGACATG GGTCTCCCAC AGCTCATGTT 
CATGAGCCCT GCACCACACC CCCTGAACCT AACTCTCGCT CCTCAAGGAA CCAAACACAT 
GGGGCAATGA AGGCAGCCAA GCCCCTTAGC ACCATTTCTG AGCCTGCCTT TGCCCAGCTC 
CCTGAGGCAC CGCCTCACAC TCCCCAGATG CCAAAGGAGG AGGCAGCTGA TGGGTCAGGC 
TTCACCCCAG AGCCCCAGCC TAGGGCCTCT CAAAACCGCA AGAGGCCTTC AGCTACTGCA 
CATTCACCTC CACTTCAAAA ACGGCTCCAG AGAGGGAGAG TCCCTCAGAA GGCAGCATCC 
CTTAAGGAAG AAGAAAATCC AGCAGTAAGG CCGAGGAAGG AAGAGGGTGT AGTGATTCCA 
GGTCCAGGCA AGAGAAGGAG AGAGCAGACA GAGGAAGAGT CCCAGGGAAG ACCGAGCCGT 
AGCCTGCGAC GGACCAAACC TGTCCAGGAG TCCACGGCCC CCAAAGTGCT CTTCACGGGC 
GTGGTGGATG CTCGCGGAGA GAGGACGGTG CTGGCCCTGG GGGGCAGTCT GGCCAGCTCA 
GTGGCTGAGG CTTCTCACCT GGTGACTGAT CGGATCCGCC GTACGGTCAA GTTTCTGTGT 
GCCCTGGGCC GGGGCATCCC CATCCTCTCC CTGGCCTGGC TGCATGAGTC CCGCAAGGCA 
GGCTGCTTCT TGCCCCCGGA CGAATATTTG GTGACTGATC CTGAGCAGGA GAAGAACTTC 
GGCTTCAGCC TTCGGGAGGC CCTGAGCCGA GCTCGGGAGC GAAGGCTGCT GGAGGGCTAT 
GAGATTCACG TGACCCCCGG AGTCCAGCCA CCGCCACCTC AGATGGGAGA AATCATCAAC 
TGCTGTGGAG GCGCCATCCT GCCCAGCATG CCTCGGTCCT ACAAGCCTCA GAGGGTCGTG 
ATCACATGTT CCCAGGACTT CCCTCGATGT GCCGTTCCAT ATCGGGTTGG GCTGCCTGTC 
CTCTCACCCG AGTTCCTGCT GACGGGAGTA CTGAAGCAGG AAGTCAAGCC AGAGGCCTTT 
GCCTTCTCCA CTGTGGAAAT GTCATCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024839899.1
CDS	199..5211
Translation	MEDTQILNWEVEEEEEVEERPSESLRCSLEPLGQLRIFSSSYGPEKDFPLYLGKNMIGRMPDCSVALPFSSISKQHAVIEISAWDKAPVLRDCGSLNGTQILRPPKVLGPGMSHRLRDRELILFADLPCQYHRLDVPRPFVSRGPLTVEETPRVQGGTQPPRLLLAEDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQADSEDLDLPATQCFADRENQSLEAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_024984131.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024984131.1

ORF Insert Sequence:

ATGGAAGACA CCCAGATTTT AAACTGGGAA GTTGAAGAAG AGGAGGAGGT TGAAGAGAGA 
CCCAGTGAAT CTCTGAGGTG TAGCTTGGAG CCCCTAGGGC AGCTGCGTAT CTTCAGTAGT 
TCCTACGGAC CAGAAAAAGA CTTCCCACTC TACCTGGGGA AGAACATGAT AGGCCGAATG 
CCTGATTGCT CTGTGGCCCT GCCCTTTTCA TCAATCTCCA AACAACATGC AGTGATTGAA 
ATCTCTGCCT GGGACAAGGC GCCTGTCCTC CGGGATTGCG GGAGCCTCAA TGGCACTCAA 
ATCCTGCGGC CTCCTAAGGT CCTGGGCCCT GGGATGAGTC ATCGTTTGCG GGACCGGGAG 
TTGATTCTCT TTGCTGACTT GCCCTGCCAG TACCATCGCT TGGATGTCCC CCGGCCTTTT 
GTCTCTCGGG GCCCTCTAAC TGTAGAGGAG ACACCCAGGG TACAGGGAGG AACTCAACCC 
CCCAGGCTTC TGTTGGCTGA GGACTCAGAG GAAGAAGTAG ATTCCCTTTT GGACAAGTGT 
GTGGTGAAAG GACCAAGGAC CTCCTCTTTG GCAACAGTCG TTCCAGAGAG TGATGAAGAA 
GGGCCTTCCC CTGCCCCAGA TGGGCCCGGG CCACCTTCTG CCTTCAACTT GAACAGCGAC 
ACAGATGAGG AAGAAAGTCA GGAATCAGGA GCAGGGGAGG CCTCTTCAGC TCCCAGAAGA 
GGTACCGCTG CAGAGACAGA ACAGCCTAAA CCTGTCACAG CCGAAATCCA GATTGAGAAG 
GATCAGTGTT CCGTGAAGGA GAGGAACAGG GACACAGAAA TCGAGAGGGA TGTGAAGAAT 
GGGGTGGTTC CGACTGGAGT GATTCTGGAG AGGAGCCAGC CTTCTGGGGA GGACAGTGAC 
ACAGATGTGA GTGATGAGAG TGGGCCTCCA AGAAGGCTTG CTGGGGTCCG TCCGAAAAGG 
GCCTGGTCTT GTAACTTCAT AGACAGTGAT ACTGATGGGG AGGACAAGGG GATCCCTGCT 
GCCCCAGCAG TGGTTCCCAT GAAGGAGAGG CAGATCTTCC ACGAAGCTGG TACACAGAGC 
CCCCAGGCAC CTGGTGTGGC ATGTCGGCAG GAGAGCCCAG CTGATGGTGA TACAGATATA 
GAGGAGGGAG AGGCCCCCCT GGACAGAAGC CAAGCCTCCA TGGTGATCGA CAGCAATACA 
GACGATGAGG AAGAAGTCTC GGCAGCACTG ACACTGGCAC GTCTAAGAGA GAGCCAGGCT 
GATTCTGAAG ATCTGGACCT ACCAGCTACC CAGTGCTTTG CAGACAGAGA GAATCAGAGC 
CTGGAAGCCC CCAGCATGGA GGATGAGCCC ACCCAGGCCT TCCTGTTTAC TCTGCCCCAA 
GAGCCTGGCC CTTCCTGTTG CAGCTCCCAG GCCACAGGTT CCCTGAATGA GGCATGGGAG 
GTCTTGGCGA CACAGCCATT CTGTCCGAGA GAGTATGAGG CCTCCGAGAC CCAAACCGCT 
GTCACCCTCC TTGACACCCG TGCATCTTGC CCCCCTCCAT CTAGGACAGC ACAGCAAGAG 
CAACATTCAG AGAGCCCAGT CCATGCAGAA GCACTGGGGA TGGAAGGCAG AGGGATGCAG 
ACCGTGGAGA AAGACATGGG TACCCAAAGA GAAACAGCAG AGAGGGTGAT CCCTGAGGGA 
GGGCCACCGC AGAATGAAAC CAAGAAACTG CCCTCGGAAG GAGAGAGGGA AGATGTGACG 
GGAGAGGAAG AATTAATCGG GGGGATACAG GGTAGAGAAC AAAATCAGGT GTTAGCTAGA 
GATACTCAGA GCCAAGAATC TGACAAAACA GTGAAAAGTG CAAGTACTGG AAGGGGAATG 
GAGATTGTAA AGTTAGAAAC TGAGACACCC AAGGAAACAC AGGAGAAAGA GAGAGAAAAG 
CAGACTCTCG CAGGGGAAAT ATTTGAGAGT GAAGCAGGGA AACTGGTAGC AGAGAGAGAG 
AGTGAGGTAG GTGGGTTAGA AGTCAAGGGA CCCCAAGAGC TACTGGACAG AGGCCCACAG 
ATGAGGGAGA CAGAGGCGGG GGGCCAGGAC CAGAAAGGCC AAGCCTCTGG TCCGCCATCA 
GAGCCTGGAG CAGGGGCAGG AGACCTTCAG GGATTTACTT CAGATCCAGT AGCTTCTGGG 
AGCCAGGCAG GTGGAGGAAG GGGAGCCCCA GGGAGCCCCA GGAGGCAGCA GAGAGGTGAC 
TTGAATTTCG AGATGCCACC TGCTGAGAAG GCTTCCAGGG GTGATCAGGA ATCCCCAGAT 
GCTTGTCTGC CTCCTGCAGC GCCTGAAGCC TCAGCCGCAC TCCCAAACTC CCTCATCTCT 
CAGATGCAAA AACATCCCGC ACCTCAGTCC CTCCTTTTTC CCTCTCCAGC TCCTTTAGAA 
CTGCCTATTC CCAGGACCAG ACGAAATGAG AGTCAGGAAG CTCCAGAGAC TCCCTTCTCC 
TCAGAGCTGA ACTCTGTCCA CCCAGAACCC AAAGTCAGGC CCCAGGGGTC CTCTCCAGTT 
TCTTCTCTAC CCCTTGAGCC CCACCCTGCC ACCCCCACAG GCCAGCCTAT TGCCCTTGAG 
CCCACATCTG GGGTCTCTGG GAGCGGGACA CATAGTTCCT TTGATGTAAC TGCCTCATCA 
GTTGTCCCCA CAGCCCTTGC ACTGCAGCCA TCCACCTCCA CAGACCAGCC TGTCACCCCT 
AAGCCCACAC TTGGGGCCCC TCGGGGCAGG GCACAGAGGT CTTCTGTCAA AACCCCTGAA 
CCCAGTGTCC GCATAGACCA GCCTATTGCC CCTGAGCTCA CAGCTAAGGC CACTCGGGGC 
AGGGCACAGA GGTCTTCTGT CAAGACTCCC AAACCAGATA ACTCCACAAC ACCCAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAGC CCACATCTCA GGCCCCTCGG 
GGCAGGACAC CTAAGTCTTC TGCCAAGACT CCTGAACCAG CTGTCCCCAC AGCCTCTGAG 
CTCCAGCCTG CTGCCCCTAA AGACCAGCCT GTTGCTCCTG AGCTCACATC TAGAGCCACT 
CGGGGCAGGA CACAGAGGTC TTCTATCAAG ACTTCCAAAC CAGATACGTC CACAGCCCCG 
AAGCCCCAGC CTTCCACTTC CACAGACCAG CCTGTCACCC CCAAACCCAC GTCTCGGGCC 
TCTCGAGGCA GGACACCTAG GTCTTCCACC AAGACTCCTG AACCAGTTGT CTCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCTCACAGAC CAGCCTATCA CTCCTGAGCT CACATCTAGG 
GCTACTCGGG GCAGGGCACA GAGATCCTCT GTCAAAACCC CTGATCCAGT TACCACCACA 
ACACCTGAGC TCCAGCCTTC CACCTCCACA GACCAGCTTG TCACCCCCAA ACGCCCATCT 
CGGGCCCCTC GAGGCAGGAC ACGTAGGTCG TCTGCCAAGA CTCCTGAACC AGTTGTTCCC 
ACAGCCTCTG AGCTCCAGCC TTCTGCCCCT GCAGACCAGC CTGTTGGTCC TTGGGCCACT 
CAGTGTAGAA GACATAGGTC TTCTGTCAAG ACCCCGGAAC CAGTTGTCCC CACAGTCCCT 
GAACCTCAGC CTTCTACTTC TAAAGACCAG TCTGTCGCTC CTGAACCCAC ATCTCAGGCC 
ACTCAGAGCC AAACACATAG GTCTTCTGTC AAGACATCCC AGCCAACTGA ACCCACAGCC 
CCTGACCTCA AACCTTCCTC CCCCACAGAC CAGCCTGTCA CTCCCAAGGT CATAGCTCAG 
GGTGGTCCAA GCAGGACACG AAGGGCTTCT ACAGCAAGTG CTGTGCTGGT TCCTACTACC 
CCTGAATTCC AGTCTCCAGT CCGCTCAGAA CAGCCTCTTT CCCCTGACCC CATCCCCGAA 
GTCAACTGCA GCCTGAGGCC GAGGGCCACT AGGAGACATG GGTCTCCCAC AGCTCATGTT 
CATGAGCCCT GCACCACACC CCCTGAACCT AACTCTCGCT CCTCAAGGAA CCAAACACAT 
GGGGCAATGA AGGCAGCCAA GCCCCTTAGC ACCATTTCTG AGCCTGCCTT TGCCCAGCTC 
CCTGAGGCAC CGCCTCACAC TCCCCAGATG CCAAAGGAGG AGGCAGCTGA TGGGTCAGGC 
TTCACCCCAG AGCCCCAGCC TAGGGCCTCT CAAAACCGCA AGAGGCCTTC AGCTACTGCA 
CATTCACCTC CACTTCAAAA ACGGCTCCAG AGAGGGAGAG TCCCTCAGAA GGCAGCATCC 
CTTAAGGAAG AAGAAAATCC AGCAGTAAGG CCGAGGAAGG AAGAGGGTGT AGTGATTCCA 
GGTCCAGGCA AGAGAAGGAG AGAGCAGACA GAGGAAGAGT CCCAGGGAAG ACCGAGCCGT 
AGCCTGCGAC GGACCAAACC TGTCCAGGAG TCCACGGCCC CCAAAGTGCT CTTCACGGGC 
GTGGTGGATG CTCGCGGAGA GAGGACGGTG CTGGCCCTGG GGGGCAGTCT GGCCAGCTCA 
GTGGCTGAGG CTTCTCACCT GGTGACTGAT CGGATCCGCC GTACGGTCAA GTTTCTGTGT 
GCCCTGGGCC GGGGCATCCC CATCCTCTCC CTGGCCTGGC TGCATGAGTC CCGCAAGGCA 
GGCTGCTTCT TGCCCCCGGA CGAATATTTG GTGACTGATC CTGAGCAGGA GAAGAACTTC 
GGCTTCAGCC TTCGGGAGGC CCTGAGCCGA GCTCGGGAGC GAAGGCTGCT GGAGGGCTAT 
GAGATTCACG TGACCCCCGG AGTCCAGCCA CCGCCACCTC AGATGGGAGA AATCATCAAC 
TGCTGTGGAG GCGCCATCCT GCCCAGCATG CCTCGGTCCT ACAAGCCTCA GAGGGTCGTG 
ATCACATGTT CCCAGGACTT CCCTCGATGT GCCGTTCCAT ATCGGGTTGG GCTGCCTGTC 
CTCTCACCCG AGTTCCTGCT GACGGGAGTA CTGAAGCAGG AAGTCAAGCC AGAGGCCTTT 
GCCTTCTCCA CTGTGGAAAT GTCATCCACC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_015468919.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015324405.1 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Price & Availability↑

CloneID	OBa32194
Clone ID Related Accession (Same CDS sequence)	XM_015468919.1 , XM_002697355.3
Accession Version	XM_015468919.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646599.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGTCGCCT TGGGGAGGGT GGGGGAACGC TGGGGGGCAG AGAACAGGAA GGAGAGGCTC 
CAGAGGGGCC CAGGAGCCCC CACCCCTCCG CCCTGCACTC CGCCCCGGGG CGGGCCAGGG 
GAAATACCCT CCCCCGGGGA CTTTCGGAAC CCGGCGATCG GAGCTGCCAG CTCGTTCCCA 
GAACCCTGGC GTCCACCCCC CACCAACCCT AACCCGGTCC CGGCCCCGCC GCCGGCCTCC 
CCCACCGGCG ATGCCCCGAG GTCCCGGGCG AGCGAGCGCC CCAGGCGTAC GGAGCACCGG 
CTCCACACCG CTTCGCTCCG GAGCCGGCCA GACTCAGAGG AAGAAGTAGA TTCCCTTTTG 
GACAAGTGTG TGGTGAAAGG ACCAAGGACC TCCTCTTTGG CAACAGTCGT TCCAGAGAGT 
GATGAAGAAG GGCCTTCCCC TGCCCCAGAT GGGCCCGGGC CACCTTCTGC CTTCAACTTG 
AACAGCGACA CAGATGAGGA AGAAAGTCAG GAATCAGGAG CAGGGGAGGC CTCTTCAGCT 
CCCAGAAGAG GTACCGCTGC AGAGACAGAA CAGCCTAAAC CTGTCACAGC CGAAATCCAG 
ATTGAGAAGG ATCAGTGTTC CGTGAAGGAG AGGAACAGGG ACACAGAAAT CGAGAGGGAT 
GTGAAGAATG GGGTGGTTCC GACTGGAGTG ATTCTGGAGA GGAGCCAGCC TTCTGGGGAG 
GACAGTGACA CAGATGTGAG TGATGAGAGT GGGCCTCCAA GAAGGCTTGC TGGGGTCCGT 
CCGAAAAGGG CCTGGTCTTG TAACTTCATA GACAGTGATA CTGATGGGGA GGACAAGGGG 
ATCCCTGCTG CCCCAGCAGT GGTTCCCATG AAGGAGAGGC AGATCTTCCA CGAAGCTGGT 
ACACAGAGCC CCCAGGCACC TGGTGTGGCA TGTCGGCAGG AGAGCCCAGC TGATGGTGAT 
ACAGATATAG AGGAGGGAGA GGCCCCCCTG GACAGAAGCC AAGCCTCCAT GGTGATCGAC 
AGCAATACAG ACGATGAGGA AGAAGTCTCG GCAGCACTGA CACTGGCACG TCTAAGAGAG 
AGCCAGGCTG GTAAGTGGAA CCGAGATCCA GATGCAGAAG AGGACAGGGC CCAACCAGTG 
GCCCTTCTGG AGCGAAGCCA GGCCTCTGCT AGGGGAGACA GTGACACAGA TGTGGAGGAA 
GAGGGGCTCC CAGTGGGAAG GAGGGGAATG GTTCCCAAGG GTCACATGGA CAGGGAATAT 
TCAAAAAAGA GCCAGCATCC TCCCAGGGAC AGTGATACAG AGGGGAAGAA AGATGAGAGC 
TCTCCGGGGG TCCACCTGGA GAGAAGCCAG GCCTCTGCAC AAGTGGAGGA CGAGGTCCCA 
CTGGGGCCAG CTGTTGCACT TCCGGAGAAA CATCAGGTAC AAGGCATAGT GTGGACACAT 
CACACCGATG CGGAGGCAGA AGGGGGCCCG GCACAGCTCC CCGTGCTGCG TCTAGAGGAA 
GCCCGGCCTC CTCTAGCTGG GGACTGTGAG CTGGATGCGG AGAACACATC CTCAGCAGCA 
GCTGGTGTCA GAAAGAGCCA GCTTCCGGCG GAAAAAGATG CTGGGACCAC GTGGGCCGCA 
GCCATTCCTG AACAGGACAG AACACTTGCC ACCAGGACCC AGGGTGGGTC ATCCACGGCA 
CCAGGGGAGC AGGACCTTCT CCCGGTCTCA AGGGAAAACC TAGCAAATCC GGTGGTGGAT 
ACAGGCACTC CACGGGAGCC CCAGCCACAG AGAGAGGGGG CCCAGACCAC CACAGGAAGG 
GAGAAAGAAC CACATGGGAA TAGGGCCACA GACTCTGGAG AGAGCCTCCA TGATTCTGAA 
GATCTGGACC TACCAGCTAC CCAGTGCTTT GCAGACAGAG AGAATCAGAG CCTGGAAGCA 
GCCCCCAGCA TGGAGGATGA GCCCACCCAG GCCTTCCTGT TTACTCTGCC CCAAGAGCCT 
GGCCCTTCCT GTTGCAGCTC CCAGGCCACA GGTTCCCTGA ATGAGGCATG GGAGGTCTTG 
GCGACACAGC CATTCTGTCC GAGAGAGTAT GAGGCCTCCG AGACCCAAAC CGCTGTCACC 
CTCCTTGACA CCCGTGCATC TTGCCCCCCT CCATCTAGGA CAGCACAGCA AGAGCAACAT 
TCAGAGAGCC CAGTCCATGC AGAAGCACTG GGGATGGAAG GCAGAGGGAT GCAGACCGTG 
GAGAAAGACA TGGGTACCCA AAGAGAAACA GCAGAGAGGG TGATCCCTGA GGGAGGGCCA 
CCGCAGAATG AAACCAAGAA ACTGCCCTCG GAAGGAGAGA GGGAAGATGT GACGGGAGAG 
GAAGAATTAA TCGGGGGGAT ACAGGGTAGA GAACAAAATC AGGTGTTAGC TAGAGATACT 
CAGAGCCAAG AATCTGACAA AACAGTGAAA AGTGCAAGTA CTGGAAGGGG AATGGAGATT 
GTAAAGTTAG AAACTGAGAC ACCCAAGGAA ACACAGGAGA AAGAGAGAGA AAAGCAGACT 
CTCGCAGGGG AAATATTTGA GAGTGAAGCA GGGAAACTGG TAGCAGAGAG AGAGAGTGAG 
GTAGGTGGGT TAGAAGTCAA GGGACCCCAA GAGCTACTGG ACAGAGGCCC ACAGATGAGG 
GAGACAGAGG CGGGGGGCCA GGACCAGAAA GGCCAAGCCT CTGGTCCGCC ATCAGAGCCT 
GGAGCAGGGG CAGGAGACCT TCAGGGATTT ACTTCAGATC CAGTAGCTTC TGGGAGCCAG 
GCAGGTGGAG GAAGGGGAGC CCCAGGGAGC CCCAGGAGGC AGCAGAGAGG TGACTTGAAT 
TTCGAGATGC CACCTGCTGA GAAGGCTTCC AGGGGTGATC AGGAATCCCC AGATGCTTGT 
CTGCCTCCTG CAGCGCCTGA AGCCTCAGCC GCACTCCCAA ACTCCCTCAT CTCTCAGATG 
CAAAAACATC CCGCACCTCA GTCCCTCCTT TTTCCCTCTC CAGCTCCTTT AGAACTGCCT 
ATTCCCAGGA CCAGACGAAA TGAGAGTCAG GAAGCTCCAG AGACTCCCTT CTCCTCAGAG 
CTGAACTCTG TCCACCCAGA ACCCAAAGTC AGGCCCCAGG GGTCCTCTCC AGTTTCTTCT 
CTACCCCTTG AGCCCCACCC TGCCACCCCC ACAGGCCAGC CTATTGCCCT TGAGCCCACA 
TCTGGGGTCT CTGGGAGCGG GACACATAGT TCCTTTGATG TAACTGCCTC ATCAGTTGTC 
CCCACAGCCC TTGCACTGCA GCCATCCACC TCCACAGACC AGCCTGTCAC CCCTAAGCCC 
ACACTTGGGG CCCCTCGGGG CAGGGCACAG AGGTCTTCTG TCAAAACCCC TGAACCCAGT 
GTCCGCATAG ACCAGCCTAT TGCCCCTGAG CTCACAGCTA AGGCCACTCG GGGCAGGGCA 
CAGAGGTCTT CTGTCAAGAC TCCCAAACCA GATAACTCCA CAACACCCAA GCCCCAGCCT 
TCCACTTCCA CAGACCAGCC TGTCACCCCC AAGCCCACAT CTCAGGCCCC TCGGGGCAGG 
ACACCTAAGT CTTCTGCCAA GACTCCTGAA CCAGCTGTCC CCACAGCCTC TGAGCTCCAG 
CCTGCTGCCC CTAAAGACCA GCCTGTTGCT CCTGAGCTCA CATCTAGAGC CACTCGGGGC 
AGGACACAGA GGTCTTCTAT CAAGACTTCC AAACCAGATA CGTCCACAGC CCCGAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAAC CCACGTCTCG GGCCTCTCGA 
GGCAGGACAC CTAGGTCTTC CACCAAGACT CCTGAACCAG TTGTCTCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCTCAC AGACCAGCCT ATCACTCCTG AGCTCACATC TAGGGCTACT 
CGGGGCAGGG CACAGAGATC CTCTGTCAAA ACCCCTGATC CAGTTACCAC CACAACACCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CTTGTCACCC CCAAACGCCC ATCTCGGGCC 
CCTCGAGGCA GGACACGTAG GTCGTCTGCC AAGACTCCTG AACCAGTTGT TCCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCCTGCAGAC CAGCCTGTTG GTCCTTGGGC CACTCAGTGT 
AGAAGACATA GGTCTTCTGT CAAGACCCCG GAACCAGTTG TCCCCACAGT CCCTGAACCT 
CAGCCTTCTA CTTCTAAAGA CCAGTCTGTC GCTCCTGAAC CCACATCTCA GGCCACTCAG 
AGCCAAACAC ATAGGTCTTC TGTCAAGACA TCCCAGCCAA CTGAACCCAC AGCCCCTGAC 
CTCAAACCTT CCTCCCCCAC AGACCAGCCT GTCACTCCCA AGGTCATAGC TCAGGGTGGT 
CCAAGCAGGA CACGAAGGGC TTCTACAGCA AGTGCTGTGC TGGTTCCTAC TACCCCTGAA 
TTCCAGTCTC CAGTCCGCTC AGAACAGCCT CTTTCCCCTG ACCCCATCCC CGAAGTCAAC 
TGCAGCCTGA GGCCGAGGGC CACTAGGAGA CATGGGTCTC CCACAGCTCA TGTTCATGAG 
CCCTGCACCA CACCCCCTGA ACCTAACTCT CGCTCCTCAA GGAACCAAAC ACATGGGGCA 
ATGAAGGCAG CCAAGCCCCT TAGCACCATT TCTGAGCCTG CCTTTGCCCA GCTCCCTGAG 
GCACCGCCTC ACACTCCCCA GATGCCAAAG GAGGAGGCAG CTGATGGGTC AGGCTTCACC 
CCAGAGCCCC AGCCTAGGGC CTCTCAAAAC CGCAAGAGGC CTTCAGCTAC TGCACATTCA 
CCTCCACTTC AAAAACGGCT CCAGAGAGGG AGAGTCCCTC AGAAGGCAGC ATCCCTTAAG 
GAAGAAGAAA ATCCAGCAGT AAGGCCGAGG AAGGAAGAGG GTGTAGTGAT TCCAGGTCCA 
GGCAAGAGAA GGAGAGAGCA GACAGAGGAA GAGTCCCAGG GAAGACCGAG CCGTAGCCTG 
CGACGGACCA AACCTGTCCA GGAGTCCACG GCCCCCAAAG TGCTCTTCAC GGGCGTGGTG 
GATGCTCGCG GAGAGAGGAC GGTGCTGGCC CTGGGGGGCA GTCTGGCCAG CTCAGTGGCT 
GAGGCTTCTC ACCTGGTGAC TGATCGGATC CGCCGTACGG TCAAGTTTCT GTGTGCCCTG 
GGCCGGGGCA TCCCCATCCT CTCCCTGGCC TGGCTGCATG AGTCCCGCAA GGCAGGCTGC 
TTCTTGCCCC CGGACGAATA TTTGGTGACT GATCCTGAGC AGGAGAAGAA CTTCGGCTTC 
AGCCTTCGGG AGGCCCTGAG CCGAGCTCGG GAGCGAAGGC TGCTGGAGGG CTATGAGATT 
CACGTGACCC CCGGAGTCCA GCCACCGCCA CCTCAGATGG GAGAAATCAT CAACTGCTGT 
GGAGGCGCCA TCCTGCCCAG CATGCCTCGG TCCTACAAGC CTCAGAGGGT CGTGATCACA 
TGTTCCCAGG ACTTCCCTCG ATGTGCCGTT CCATATCGGG TTGGGCTGCC TGTCCTCTCA 
CCCGAGTTCC TGCTGACGGG AGTACTGAAG CAGGAAGTCA AGCCAGAGGC CTTTGCCTTC 
TCCACTGTGG AAATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015324405.1
CDS	1..5607
Translation	MVALGRVGERWGAENRKERLQRGPGAPTPPPCTPPRGGPGEIPSPGDFRNPAIGAASSFPEPWRPPPTNPNPVPAPPPASPTGDAPRSRASERPRRTEHRLHTASLRSRPDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_015468919.1
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015468919.1

ORF Insert Sequence:

ATGGTCGCCT TGGGGAGGGT GGGGGAACGC TGGGGGGCAG AGAACAGGAA GGAGAGGCTC 
CAGAGGGGCC CAGGAGCCCC CACCCCTCCG CCCTGCACTC CGCCCCGGGG CGGGCCAGGG 
GAAATACCCT CCCCCGGGGA CTTTCGGAAC CCGGCGATCG GAGCTGCCAG CTCGTTCCCA 
GAACCCTGGC GTCCACCCCC CACCAACCCT AACCCGGTCC CGGCCCCGCC GCCGGCCTCC 
CCCACCGGCG ATGCCCCGAG GTCCCGGGCG AGCGAGCGCC CCAGGCGTAC GGAGCACCGG 
CTCCACACCG CTTCGCTCCG GAGCCGGCCA GACTCAGAGG AAGAAGTAGA TTCCCTTTTG 
GACAAGTGTG TGGTGAAAGG ACCAAGGACC TCCTCTTTGG CAACAGTCGT TCCAGAGAGT 
GATGAAGAAG GGCCTTCCCC TGCCCCAGAT GGGCCCGGGC CACCTTCTGC CTTCAACTTG 
AACAGCGACA CAGATGAGGA AGAAAGTCAG GAATCAGGAG CAGGGGAGGC CTCTTCAGCT 
CCCAGAAGAG GTACCGCTGC AGAGACAGAA CAGCCTAAAC CTGTCACAGC CGAAATCCAG 
ATTGAGAAGG ATCAGTGTTC CGTGAAGGAG AGGAACAGGG ACACAGAAAT CGAGAGGGAT 
GTGAAGAATG GGGTGGTTCC GACTGGAGTG ATTCTGGAGA GGAGCCAGCC TTCTGGGGAG 
GACAGTGACA CAGATGTGAG TGATGAGAGT GGGCCTCCAA GAAGGCTTGC TGGGGTCCGT 
CCGAAAAGGG CCTGGTCTTG TAACTTCATA GACAGTGATA CTGATGGGGA GGACAAGGGG 
ATCCCTGCTG CCCCAGCAGT GGTTCCCATG AAGGAGAGGC AGATCTTCCA CGAAGCTGGT 
ACACAGAGCC CCCAGGCACC TGGTGTGGCA TGTCGGCAGG AGAGCCCAGC TGATGGTGAT 
ACAGATATAG AGGAGGGAGA GGCCCCCCTG GACAGAAGCC AAGCCTCCAT GGTGATCGAC 
AGCAATACAG ACGATGAGGA AGAAGTCTCG GCAGCACTGA CACTGGCACG TCTAAGAGAG 
AGCCAGGCTG GTAAGTGGAA CCGAGATCCA GATGCAGAAG AGGACAGGGC CCAACCAGTG 
GCCCTTCTGG AGCGAAGCCA GGCCTCTGCT AGGGGAGACA GTGACACAGA TGTGGAGGAA 
GAGGGGCTCC CAGTGGGAAG GAGGGGAATG GTTCCCAAGG GTCACATGGA CAGGGAATAT 
TCAAAAAAGA GCCAGCATCC TCCCAGGGAC AGTGATACAG AGGGGAAGAA AGATGAGAGC 
TCTCCGGGGG TCCACCTGGA GAGAAGCCAG GCCTCTGCAC AAGTGGAGGA CGAGGTCCCA 
CTGGGGCCAG CTGTTGCACT TCCGGAGAAA CATCAGGTAC AAGGCATAGT GTGGACACAT 
CACACCGATG CGGAGGCAGA AGGGGGCCCG GCACAGCTCC CCGTGCTGCG TCTAGAGGAA 
GCCCGGCCTC CTCTAGCTGG GGACTGTGAG CTGGATGCGG AGAACACATC CTCAGCAGCA 
GCTGGTGTCA GAAAGAGCCA GCTTCCGGCG GAAAAAGATG CTGGGACCAC GTGGGCCGCA 
GCCATTCCTG AACAGGACAG AACACTTGCC ACCAGGACCC AGGGTGGGTC ATCCACGGCA 
CCAGGGGAGC AGGACCTTCT CCCGGTCTCA AGGGAAAACC TAGCAAATCC GGTGGTGGAT 
ACAGGCACTC CACGGGAGCC CCAGCCACAG AGAGAGGGGG CCCAGACCAC CACAGGAAGG 
GAGAAAGAAC CACATGGGAA TAGGGCCACA GACTCTGGAG AGAGCCTCCA TGATTCTGAA 
GATCTGGACC TACCAGCTAC CCAGTGCTTT GCAGACAGAG AGAATCAGAG CCTGGAAGCA 
GCCCCCAGCA TGGAGGATGA GCCCACCCAG GCCTTCCTGT TTACTCTGCC CCAAGAGCCT 
GGCCCTTCCT GTTGCAGCTC CCAGGCCACA GGTTCCCTGA ATGAGGCATG GGAGGTCTTG 
GCGACACAGC CATTCTGTCC GAGAGAGTAT GAGGCCTCCG AGACCCAAAC CGCTGTCACC 
CTCCTTGACA CCCGTGCATC TTGCCCCCCT CCATCTAGGA CAGCACAGCA AGAGCAACAT 
TCAGAGAGCC CAGTCCATGC AGAAGCACTG GGGATGGAAG GCAGAGGGAT GCAGACCGTG 
GAGAAAGACA TGGGTACCCA AAGAGAAACA GCAGAGAGGG TGATCCCTGA GGGAGGGCCA 
CCGCAGAATG AAACCAAGAA ACTGCCCTCG GAAGGAGAGA GGGAAGATGT GACGGGAGAG 
GAAGAATTAA TCGGGGGGAT ACAGGGTAGA GAACAAAATC AGGTGTTAGC TAGAGATACT 
CAGAGCCAAG AATCTGACAA AACAGTGAAA AGTGCAAGTA CTGGAAGGGG AATGGAGATT 
GTAAAGTTAG AAACTGAGAC ACCCAAGGAA ACACAGGAGA AAGAGAGAGA AAAGCAGACT 
CTCGCAGGGG AAATATTTGA GAGTGAAGCA GGGAAACTGG TAGCAGAGAG AGAGAGTGAG 
GTAGGTGGGT TAGAAGTCAA GGGACCCCAA GAGCTACTGG ACAGAGGCCC ACAGATGAGG 
GAGACAGAGG CGGGGGGCCA GGACCAGAAA GGCCAAGCCT CTGGTCCGCC ATCAGAGCCT 
GGAGCAGGGG CAGGAGACCT TCAGGGATTT ACTTCAGATC CAGTAGCTTC TGGGAGCCAG 
GCAGGTGGAG GAAGGGGAGC CCCAGGGAGC CCCAGGAGGC AGCAGAGAGG TGACTTGAAT 
TTCGAGATGC CACCTGCTGA GAAGGCTTCC AGGGGTGATC AGGAATCCCC AGATGCTTGT 
CTGCCTCCTG CAGCGCCTGA AGCCTCAGCC GCACTCCCAA ACTCCCTCAT CTCTCAGATG 
CAAAAACATC CCGCACCTCA GTCCCTCCTT TTTCCCTCTC CAGCTCCTTT AGAACTGCCT 
ATTCCCAGGA CCAGACGAAA TGAGAGTCAG GAAGCTCCAG AGACTCCCTT CTCCTCAGAG 
CTGAACTCTG TCCACCCAGA ACCCAAAGTC AGGCCCCAGG GGTCCTCTCC AGTTTCTTCT 
CTACCCCTTG AGCCCCACCC TGCCACCCCC ACAGGCCAGC CTATTGCCCT TGAGCCCACA 
TCTGGGGTCT CTGGGAGCGG GACACATAGT TCCTTTGATG TAACTGCCTC ATCAGTTGTC 
CCCACAGCCC TTGCACTGCA GCCATCCACC TCCACAGACC AGCCTGTCAC CCCTAAGCCC 
ACACTTGGGG CCCCTCGGGG CAGGGCACAG AGGTCTTCTG TCAAAACCCC TGAACCCAGT 
GTCCGCATAG ACCAGCCTAT TGCCCCTGAG CTCACAGCTA AGGCCACTCG GGGCAGGGCA 
CAGAGGTCTT CTGTCAAGAC TCCCAAACCA GATAACTCCA CAACACCCAA GCCCCAGCCT 
TCCACTTCCA CAGACCAGCC TGTCACCCCC AAGCCCACAT CTCAGGCCCC TCGGGGCAGG 
ACACCTAAGT CTTCTGCCAA GACTCCTGAA CCAGCTGTCC CCACAGCCTC TGAGCTCCAG 
CCTGCTGCCC CTAAAGACCA GCCTGTTGCT CCTGAGCTCA CATCTAGAGC CACTCGGGGC 
AGGACACAGA GGTCTTCTAT CAAGACTTCC AAACCAGATA CGTCCACAGC CCCGAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAAC CCACGTCTCG GGCCTCTCGA 
GGCAGGACAC CTAGGTCTTC CACCAAGACT CCTGAACCAG TTGTCTCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCTCAC AGACCAGCCT ATCACTCCTG AGCTCACATC TAGGGCTACT 
CGGGGCAGGG CACAGAGATC CTCTGTCAAA ACCCCTGATC CAGTTACCAC CACAACACCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CTTGTCACCC CCAAACGCCC ATCTCGGGCC 
CCTCGAGGCA GGACACGTAG GTCGTCTGCC AAGACTCCTG AACCAGTTGT TCCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCCTGCAGAC CAGCCTGTTG GTCCTTGGGC CACTCAGTGT 
AGAAGACATA GGTCTTCTGT CAAGACCCCG GAACCAGTTG TCCCCACAGT CCCTGAACCT 
CAGCCTTCTA CTTCTAAAGA CCAGTCTGTC GCTCCTGAAC CCACATCTCA GGCCACTCAG 
AGCCAAACAC ATAGGTCTTC TGTCAAGACA TCCCAGCCAA CTGAACCCAC AGCCCCTGAC 
CTCAAACCTT CCTCCCCCAC AGACCAGCCT GTCACTCCCA AGGTCATAGC TCAGGGTGGT 
CCAAGCAGGA CACGAAGGGC TTCTACAGCA AGTGCTGTGC TGGTTCCTAC TACCCCTGAA 
TTCCAGTCTC CAGTCCGCTC AGAACAGCCT CTTTCCCCTG ACCCCATCCC CGAAGTCAAC 
TGCAGCCTGA GGCCGAGGGC CACTAGGAGA CATGGGTCTC CCACAGCTCA TGTTCATGAG 
CCCTGCACCA CACCCCCTGA ACCTAACTCT CGCTCCTCAA GGAACCAAAC ACATGGGGCA 
ATGAAGGCAG CCAAGCCCCT TAGCACCATT TCTGAGCCTG CCTTTGCCCA GCTCCCTGAG 
GCACCGCCTC ACACTCCCCA GATGCCAAAG GAGGAGGCAG CTGATGGGTC AGGCTTCACC 
CCAGAGCCCC AGCCTAGGGC CTCTCAAAAC CGCAAGAGGC CTTCAGCTAC TGCACATTCA 
CCTCCACTTC AAAAACGGCT CCAGAGAGGG AGAGTCCCTC AGAAGGCAGC ATCCCTTAAG 
GAAGAAGAAA ATCCAGCAGT AAGGCCGAGG AAGGAAGAGG GTGTAGTGAT TCCAGGTCCA 
GGCAAGAGAA GGAGAGAGCA GACAGAGGAA GAGTCCCAGG GAAGACCGAG CCGTAGCCTG 
CGACGGACCA AACCTGTCCA GGAGTCCACG GCCCCCAAAG TGCTCTTCAC GGGCGTGGTG 
GATGCTCGCG GAGAGAGGAC GGTGCTGGCC CTGGGGGGCA GTCTGGCCAG CTCAGTGGCT 
GAGGCTTCTC ACCTGGTGAC TGATCGGATC CGCCGTACGG TCAAGTTTCT GTGTGCCCTG 
GGCCGGGGCA TCCCCATCCT CTCCCTGGCC TGGCTGCATG AGTCCCGCAA GGCAGGCTGC 
TTCTTGCCCC CGGACGAATA TTTGGTGACT GATCCTGAGC AGGAGAAGAA CTTCGGCTTC 
AGCCTTCGGG AGGCCCTGAG CCGAGCTCGG GAGCGAAGGC TGCTGGAGGG CTATGAGATT 
CACGTGACCC CCGGAGTCCA GCCACCGCCA CCTCAGATGG GAGAAATCAT CAACTGCTGT 
GGAGGCGCCA TCCTGCCCAG CATGCCTCGG TCCTACAAGC CTCAGAGGGT CGTGATCACA 
TGTTCCCAGG ACTTCCCTCG ATGTGCCGTT CCATATCGGG TTGGGCTGCC TGTCCTCTCA 
CCCGAGTTCC TGCTGACGGG AGTACTGAAG CAGGAAGTCA AGCCAGAGGC CTTTGCCTTC 
TCCACTGTGG AAATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MDC1 ( XM_002697355.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002697401.2 Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.

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CloneID	OBa32194
Clone ID Related Accession (Same CDS sequence)	XM_015468919.1 , XM_002697355.3
Accession Version	XM_002697355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5607bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	mediator of DNA damage checkpoint protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104557.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002697355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 5% of CDS bases ##RefSeq-Attributes-END##

ATGGTCGCCT TGGGGAGGGT GGGGGAACGC TGGGGGGCAG AGAACAGGAA GGAGAGGCTC 
CAGAGGGGCC CAGGAGCCCC CACCCCTCCG CCCTGCACTC CGCCCCGGGG CGGGCCAGGG 
GAAATACCCT CCCCCGGGGA CTTTCGGAAC CCGGCGATCG GAGCTGCCAG CTCGTTCCCA 
GAACCCTGGC GTCCACCCCC CACCAACCCT AACCCGGTCC CGGCCCCGCC GCCGGCCTCC 
CCCACCGGCG ATGCCCCGAG GTCCCGGGCG AGCGAGCGCC CCAGGCGTAC GGAGCACCGG 
CTCCACACCG CTTCGCTCCG GAGCCGGCCA GACTCAGAGG AAGAAGTAGA TTCCCTTTTG 
GACAAGTGTG TGGTGAAAGG ACCAAGGACC TCCTCTTTGG CAACAGTCGT TCCAGAGAGT 
GATGAAGAAG GGCCTTCCCC TGCCCCAGAT GGGCCCGGGC CACCTTCTGC CTTCAACTTG 
AACAGCGACA CAGATGAGGA AGAAAGTCAG GAATCAGGAG CAGGGGAGGC CTCTTCAGCT 
CCCAGAAGAG GTACCGCTGC AGAGACAGAA CAGCCTAAAC CTGTCACAGC CGAAATCCAG 
ATTGAGAAGG ATCAGTGTTC CGTGAAGGAG AGGAACAGGG ACACAGAAAT CGAGAGGGAT 
GTGAAGAATG GGGTGGTTCC GACTGGAGTG ATTCTGGAGA GGAGCCAGCC TTCTGGGGAG 
GACAGTGACA CAGATGTGAG TGATGAGAGT GGGCCTCCAA GAAGGCTTGC TGGGGTCCGT 
CCGAAAAGGG CCTGGTCTTG TAACTTCATA GACAGTGATA CTGATGGGGA GGACAAGGGG 
ATCCCTGCTG CCCCAGCAGT GGTTCCCATG AAGGAGAGGC AGATCTTCCA CGAAGCTGGT 
ACACAGAGCC CCCAGGCACC TGGTGTGGCA TGTCGGCAGG AGAGCCCAGC TGATGGTGAT 
ACAGATATAG AGGAGGGAGA GGCCCCCCTG GACAGAAGCC AAGCCTCCAT GGTGATCGAC 
AGCAATACAG ACGATGAGGA AGAAGTCTCG GCAGCACTGA CACTGGCACG TCTAAGAGAG 
AGCCAGGCTG GTAAGTGGAA CCGAGATCCA GATGCAGAAG AGGACAGGGC CCAACCAGTG 
GCCCTTCTGG AGCGAAGCCA GGCCTCTGCT AGGGGAGACA GTGACACAGA TGTGGAGGAA 
GAGGGGCTCC CAGTGGGAAG GAGGGGAATG GTTCCCAAGG GTCACATGGA CAGGGAATAT 
TCAAAAAAGA GCCAGCATCC TCCCAGGGAC AGTGATACAG AGGGGAAGAA AGATGAGAGC 
TCTCCGGGGG TCCACCTGGA GAGAAGCCAG GCCTCTGCAC AAGTGGAGGA CGAGGTCCCA 
CTGGGGCCAG CTGTTGCACT TCCGGAGAAA CATCAGGTAC AAGGCATAGT GTGGACACAT 
CACACCGATG CGGAGGCAGA AGGGGGCCCG GCACAGCTCC CCGTGCTGCG TCTAGAGGAA 
GCCCGGCCTC CTCTAGCTGG GGACTGTGAG CTGGATGCGG AGAACACATC CTCAGCAGCA 
GCTGGTGTCA GAAAGAGCCA GCTTCCGGCG GAAAAAGATG CTGGGACCAC GTGGGCCGCA 
GCCATTCCTG AACAGGACAG AACACTTGCC ACCAGGACCC AGGGTGGGTC ATCCACGGCA 
CCAGGGGAGC AGGACCTTCT CCCGGTCTCA AGGGAAAACC TAGCAAATCC GGTGGTGGAT 
ACAGGCACTC CACGGGAGCC CCAGCCACAG AGAGAGGGGG CCCAGACCAC CACAGGAAGG 
GAGAAAGAAC CACATGGGAA TAGGGCCACA GACTCTGGAG AGAGCCTCCA TGATTCTGAA 
GATCTGGACC TACCAGCTAC CCAGTGCTTT GCAGACAGAG AGAATCAGAG CCTGGAAGCA 
GCCCCCAGCA TGGAGGATGA GCCCACCCAG GCCTTCCTGT TTACTCTGCC CCAAGAGCCT 
GGCCCTTCCT GTTGCAGCTC CCAGGCCACA GGTTCCCTGA ATGAGGCATG GGAGGTCTTG 
GCGACACAGC CATTCTGTCC GAGAGAGTAT GAGGCCTCCG AGACCCAAAC CGCTGTCACC 
CTCCTTGACA CCCGTGCATC TTGCCCCCCT CCATCTAGGA CAGCACAGCA AGAGCAACAT 
TCAGAGAGCC CAGTCCATGC AGAAGCACTG GGGATGGAAG GCAGAGGGAT GCAGACCGTG 
GAGAAAGACA TGGGTACCCA AAGAGAAACA GCAGAGAGGG TGATCCCTGA GGGAGGGCCA 
CCGCAGAATG AAACCAAGAA ACTGCCCTCG GAAGGAGAGA GGGAAGATGT GACGGGAGAG 
GAAGAATTAA TCGGGGGGAT ACAGGGTAGA GAACAAAATC AGGTGTTAGC TAGAGATACT 
CAGAGCCAAG AATCTGACAA AACAGTGAAA AGTGCAAGTA CTGGAAGGGG AATGGAGATT 
GTAAAGTTAG AAACTGAGAC ACCCAAGGAA ACACAGGAGA AAGAGAGAGA AAAGCAGACT 
CTCGCAGGGG AAATATTTGA GAGTGAAGCA GGGAAACTGG TAGCAGAGAG AGAGAGTGAG 
GTAGGTGGGT TAGAAGTCAA GGGACCCCAA GAGCTACTGG ACAGAGGCCC ACAGATGAGG 
GAGACAGAGG CGGGGGGCCA GGACCAGAAA GGCCAAGCCT CTGGTCCGCC ATCAGAGCCT 
GGAGCAGGGG CAGGAGACCT TCAGGGATTT ACTTCAGATC CAGTAGCTTC TGGGAGCCAG 
GCAGGTGGAG GAAGGGGAGC CCCAGGGAGC CCCAGGAGGC AGCAGAGAGG TGACTTGAAT 
TTCGAGATGC CACCTGCTGA GAAGGCTTCC AGGGGTGATC AGGAATCCCC AGATGCTTGT 
CTGCCTCCTG CAGCGCCTGA AGCCTCAGCC GCACTCCCAA ACTCCCTCAT CTCTCAGATG 
CAAAAACATC CCGCACCTCA GTCCCTCCTT TTTCCCTCTC CAGCTCCTTT AGAACTGCCT 
ATTCCCAGGA CCAGACGAAA TGAGAGTCAG GAAGCTCCAG AGACTCCCTT CTCCTCAGAG 
CTGAACTCTG TCCACCCAGA ACCCAAAGTC AGGCCCCAGG GGTCCTCTCC AGTTTCTTCT 
CTACCCCTTG AGCCCCACCC TGCCACCCCC ACAGGCCAGC CTATTGCCCT TGAGCCCACA 
TCTGGGGTCT CTGGGAGCGG GACACATAGT TCCTTTGATG TAACTGCCTC ATCAGTTGTC 
CCCACAGCCC TTGCACTGCA GCCATCCACC TCCACAGACC AGCCTGTCAC CCCTAAGCCC 
ACACTTGGGG CCCCTCGGGG CAGGGCACAG AGGTCTTCTG TCAAAACCCC TGAACCCAGT 
GTCCGCATAG ACCAGCCTAT TGCCCCTGAG CTCACAGCTA AGGCCACTCG GGGCAGGGCA 
CAGAGGTCTT CTGTCAAGAC TCCCAAACCA GATAACTCCA CAACACCCAA GCCCCAGCCT 
TCCACTTCCA CAGACCAGCC TGTCACCCCC AAGCCCACAT CTCAGGCCCC TCGGGGCAGG 
ACACCTAAGT CTTCTGCCAA GACTCCTGAA CCAGCTGTCC CCACAGCCTC TGAGCTCCAG 
CCTGCTGCCC CTAAAGACCA GCCTGTTGCT CCTGAGCTCA CATCTAGAGC CACTCGGGGC 
AGGACACAGA GGTCTTCTAT CAAGACTTCC AAACCAGATA CGTCCACAGC CCCGAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAAC CCACGTCTCG GGCCTCTCGA 
GGCAGGACAC CTAGGTCTTC CACCAAGACT CCTGAACCAG TTGTCTCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCTCAC AGACCAGCCT ATCACTCCTG AGCTCACATC TAGGGCTACT 
CGGGGCAGGG CACAGAGATC CTCTGTCAAA ACCCCTGATC CAGTTACCAC CACAACACCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CTTGTCACCC CCAAACGCCC ATCTCGGGCC 
CCTCGAGGCA GGACACGTAG GTCGTCTGCC AAGACTCCTG AACCAGTTGT TCCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCCTGCAGAC CAGCCTGTTG GTCCTTGGGC CACTCAGTGT 
AGAAGACATA GGTCTTCTGT CAAGACCCCG GAACCAGTTG TCCCCACAGT CCCTGAACCT 
CAGCCTTCTA CTTCTAAAGA CCAGTCTGTC GCTCCTGAAC CCACATCTCA GGCCACTCAG 
AGCCAAACAC ATAGGTCTTC TGTCAAGACA TCCCAGCCAA CTGAACCCAC AGCCCCTGAC 
CTCAAACCTT CCTCCCCCAC AGACCAGCCT GTCACTCCCA AGGTCATAGC TCAGGGTGGT 
CCAAGCAGGA CACGAAGGGC TTCTACAGCA AGTGCTGTGC TGGTTCCTAC TACCCCTGAA 
TTCCAGTCTC CAGTCCGCTC AGAACAGCCT CTTTCCCCTG ACCCCATCCC CGAAGTCAAC 
TGCAGCCTGA GGCCGAGGGC CACTAGGAGA CATGGGTCTC CCACAGCTCA TGTTCATGAG 
CCCTGCACCA CACCCCCTGA ACCTAACTCT CGCTCCTCAA GGAACCAAAC ACATGGGGCA 
ATGAAGGCAG CCAAGCCCCT TAGCACCATT TCTGAGCCTG CCTTTGCCCA GCTCCCTGAG 
GCACCGCCTC ACACTCCCCA GATGCCAAAG GAGGAGGCAG CTGATGGGTC AGGCTTCACC 
CCAGAGCCCC AGCCTAGGGC CTCTCAAAAC CGCAAGAGGC CTTCAGCTAC TGCACATTCA 
CCTCCACTTC AAAAACGGCT CCAGAGAGGG AGAGTCCCTC AGAAGGCAGC ATCCCTTAAG 
GAAGAAGAAA ATCCAGCAGT AAGGCCGAGG AAGGAAGAGG GTGTAGTGAT TCCAGGTCCA 
GGCAAGAGAA GGAGAGAGCA GACAGAGGAA GAGTCCCAGG GAAGACCGAG CCGTAGCCTG 
CGACGGACCA AACCTGTCCA GGAGTCCACG GCCCCCAAAG TGCTCTTCAC GGGCGTGGTG 
GATGCTCGCG GAGAGAGGAC GGTGCTGGCC CTGGGGGGCA GTCTGGCCAG CTCAGTGGCT 
GAGGCTTCTC ACCTGGTGAC TGATCGGATC CGCCGTACGG TCAAGTTTCT GTGTGCCCTG 
GGCCGGGGCA TCCCCATCCT CTCCCTGGCC TGGCTGCATG AGTCCCGCAA GGCAGGCTGC 
TTCTTGCCCC CGGACGAATA TTTGGTGACT GATCCTGAGC AGGAGAAGAA CTTCGGCTTC 
AGCCTTCGGG AGGCCCTGAG CCGAGCTCGG GAGCGAAGGC TGCTGGAGGG CTATGAGATT 
CACGTGACCC CCGGAGTCCA GCCACCGCCA CCTCAGATGG GAGAAATCAT CAACTGCTGT 
GGAGGCGCCA TCCTGCCCAG CATGCCTCGG TCCTACAAGC CTCAGAGGGT CGTGATCACA 
TGTTCCCAGG ACTTCCCTCG ATGTGCCGTT CCATATCGGG TTGGGCTGCC TGTCCTCTCA 
CCCGAGTTCC TGCTGACGGG AGTACTGAAG CAGGAAGTCA AGCCAGAGGC CTTTGCCTTC 
TCCACTGTGG AAATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002697401.2
CDS	1..5607
Translation	MVALGRVGERWGAENRKERLQRGPGAPTPPPCTPPRGGPGEIPSPGDFRNPAIGAASSFPEPWRPPPTNPNPVPAPPPASPTGDAPRSRASERPRRTEHRLHTASLRSRPDSEEEVDSLLDKCVVKGPRTSSLATVVPESDEEGPSPAPDGPGPPSAFNLNSDTDEEESQESGAGEASSAPRRGTAAETEQPKPVTAEIQIEKDQCSVKERNRDTEIERDVKNGVVPTGVILERSQPSGEDSDTDVSDESGPPRRLAGVRPKRAWSCNFIDSDTDGEDKGIPAAPAVVPMKERQIFHEAGTQSPQAPGVACRQESPADGDTDIEEGEAPLDRSQASMVIDSNTDDEEEVSAALTLARLRESQAGKWNRDPDAEEDRAQPVALLERSQASARGDSDTDVEEEGLPVGRRGMVPKGHMDREYSKKSQHPPRDSDTEGKKDESSPGVHLERSQASAQVEDEVPLGPAVALPEKHQVQGIVWTHHTDAEAEGGPAQLPVLRLEEARPPLAGDCELDAENTSSAAAGVRKSQLPAEKDAGTTWAAAIPEQDRTLATRTQGGSSTAPGEQDLLPVSRENLANPVVDTGTPREPQPQREGAQTTTGREKEPHGNRATDSGESLHDSEDLDLPATQCFADRENQSLEAAPSMEDEPTQAFLFTLPQEPGPSCCSSQATGSLNEAWEVLATQPFCPREYEASETQTAVTLLDTRASCPPPSRTAQQEQHSESPVHAEALGMEGRGMQTVEKDMGTQRETAERVIPEGGPPQNETKKLPSEGEREDVTGEEELIGGIQGREQNQVLARDTQSQESDKTVKSASTGRGMEIVKLETETPKETQEKEREKQTLAGEIFESEAGKLVAERESEVGGLEVKGPQELLDRGPQMRETEAGGQDQKGQASGPPSEPGAGAGDLQGFTSDPVASGSQAGGGRGAPGSPRRQQRGDLNFEMPPAEKASRGDQESPDACLPPAAPEASAALPNSLISQMQKHPAPQSLLFPSPAPLELPIPRTRRNESQEAPETPFSSELNSVHPEPKVRPQGSSPVSSLPLEPHPATPTGQPIALEPTSGVSGSGTHSSFDVTASSVVPTALALQPSTSTDQPVTPKPTLGAPRGRAQRSSVKTPEPSVRIDQPIAPELTAKATRGRAQRSSVKTPKPDNSTTPKPQPSTSTDQPVTPKPTSQAPRGRTPKSSAKTPEPAVPTASELQPAAPKDQPVAPELTSRATRGRTQRSSIKTSKPDTSTAPKPQPSTSTDQPVTPKPTSRASRGRTPRSSTKTPEPVVSTASELQPSALTDQPITPELTSRATRGRAQRSSVKTPDPVTTTTPELQPSTSTDQLVTPKRPSRAPRGRTRRSSAKTPEPVVPTASELQPSAPADQPVGPWATQCRRHRSSVKTPEPVVPTVPEPQPSTSKDQSVAPEPTSQATQSQTHRSSVKTSQPTEPTAPDLKPSSPTDQPVTPKVIAQGGPSRTRRASTASAVLVPTTPEFQSPVRSEQPLSPDPIPEVNCSLRPRATRRHGSPTAHVHEPCTTPPEPNSRSSRNQTHGAMKAAKPLSTISEPAFAQLPEAPPHTPQMPKEEAADGSGFTPEPQPRASQNRKRPSATAHSPPLQKRLQRGRVPQKAASLKEEENPAVRPRKEEGVVIPGPGKRRREQTEEESQGRPSRSLRRTKPVQESTAPKVLFTGVVDARGERTVLALGGSLASSVAEASHLVTDRIRRTVKFLCALGRGIPILSLAWLHESRKAGCFLPPDEYLVTDPEQEKNFGFSLREALSRARERRLLEGYEIHVTPGVQPPPPQMGEIINCCGGAILPSMPRSYKPQRVVITCSQDFPRCAVPYRVGLPVLSPEFLLTGVLKQEVKPEAFAFSTVEMSST

Target ORF information:

RefSeq Version	XM_002697355.3
Organism	Bos taurus(cattle)
Definition	Bos taurus mediator of DNA damage checkpoint 1 (MDC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002697355.3

ORF Insert Sequence:

ATGGTCGCCT TGGGGAGGGT GGGGGAACGC TGGGGGGCAG AGAACAGGAA GGAGAGGCTC 
CAGAGGGGCC CAGGAGCCCC CACCCCTCCG CCCTGCACTC CGCCCCGGGG CGGGCCAGGG 
GAAATACCCT CCCCCGGGGA CTTTCGGAAC CCGGCGATCG GAGCTGCCAG CTCGTTCCCA 
GAACCCTGGC GTCCACCCCC CACCAACCCT AACCCGGTCC CGGCCCCGCC GCCGGCCTCC 
CCCACCGGCG ATGCCCCGAG GTCCCGGGCG AGCGAGCGCC CCAGGCGTAC GGAGCACCGG 
CTCCACACCG CTTCGCTCCG GAGCCGGCCA GACTCAGAGG AAGAAGTAGA TTCCCTTTTG 
GACAAGTGTG TGGTGAAAGG ACCAAGGACC TCCTCTTTGG CAACAGTCGT TCCAGAGAGT 
GATGAAGAAG GGCCTTCCCC TGCCCCAGAT GGGCCCGGGC CACCTTCTGC CTTCAACTTG 
AACAGCGACA CAGATGAGGA AGAAAGTCAG GAATCAGGAG CAGGGGAGGC CTCTTCAGCT 
CCCAGAAGAG GTACCGCTGC AGAGACAGAA CAGCCTAAAC CTGTCACAGC CGAAATCCAG 
ATTGAGAAGG ATCAGTGTTC CGTGAAGGAG AGGAACAGGG ACACAGAAAT CGAGAGGGAT 
GTGAAGAATG GGGTGGTTCC GACTGGAGTG ATTCTGGAGA GGAGCCAGCC TTCTGGGGAG 
GACAGTGACA CAGATGTGAG TGATGAGAGT GGGCCTCCAA GAAGGCTTGC TGGGGTCCGT 
CCGAAAAGGG CCTGGTCTTG TAACTTCATA GACAGTGATA CTGATGGGGA GGACAAGGGG 
ATCCCTGCTG CCCCAGCAGT GGTTCCCATG AAGGAGAGGC AGATCTTCCA CGAAGCTGGT 
ACACAGAGCC CCCAGGCACC TGGTGTGGCA TGTCGGCAGG AGAGCCCAGC TGATGGTGAT 
ACAGATATAG AGGAGGGAGA GGCCCCCCTG GACAGAAGCC AAGCCTCCAT GGTGATCGAC 
AGCAATACAG ACGATGAGGA AGAAGTCTCG GCAGCACTGA CACTGGCACG TCTAAGAGAG 
AGCCAGGCTG GTAAGTGGAA CCGAGATCCA GATGCAGAAG AGGACAGGGC CCAACCAGTG 
GCCCTTCTGG AGCGAAGCCA GGCCTCTGCT AGGGGAGACA GTGACACAGA TGTGGAGGAA 
GAGGGGCTCC CAGTGGGAAG GAGGGGAATG GTTCCCAAGG GTCACATGGA CAGGGAATAT 
TCAAAAAAGA GCCAGCATCC TCCCAGGGAC AGTGATACAG AGGGGAAGAA AGATGAGAGC 
TCTCCGGGGG TCCACCTGGA GAGAAGCCAG GCCTCTGCAC AAGTGGAGGA CGAGGTCCCA 
CTGGGGCCAG CTGTTGCACT TCCGGAGAAA CATCAGGTAC AAGGCATAGT GTGGACACAT 
CACACCGATG CGGAGGCAGA AGGGGGCCCG GCACAGCTCC CCGTGCTGCG TCTAGAGGAA 
GCCCGGCCTC CTCTAGCTGG GGACTGTGAG CTGGATGCGG AGAACACATC CTCAGCAGCA 
GCTGGTGTCA GAAAGAGCCA GCTTCCGGCG GAAAAAGATG CTGGGACCAC GTGGGCCGCA 
GCCATTCCTG AACAGGACAG AACACTTGCC ACCAGGACCC AGGGTGGGTC ATCCACGGCA 
CCAGGGGAGC AGGACCTTCT CCCGGTCTCA AGGGAAAACC TAGCAAATCC GGTGGTGGAT 
ACAGGCACTC CACGGGAGCC CCAGCCACAG AGAGAGGGGG CCCAGACCAC CACAGGAAGG 
GAGAAAGAAC CACATGGGAA TAGGGCCACA GACTCTGGAG AGAGCCTCCA TGATTCTGAA 
GATCTGGACC TACCAGCTAC CCAGTGCTTT GCAGACAGAG AGAATCAGAG CCTGGAAGCA 
GCCCCCAGCA TGGAGGATGA GCCCACCCAG GCCTTCCTGT TTACTCTGCC CCAAGAGCCT 
GGCCCTTCCT GTTGCAGCTC CCAGGCCACA GGTTCCCTGA ATGAGGCATG GGAGGTCTTG 
GCGACACAGC CATTCTGTCC GAGAGAGTAT GAGGCCTCCG AGACCCAAAC CGCTGTCACC 
CTCCTTGACA CCCGTGCATC TTGCCCCCCT CCATCTAGGA CAGCACAGCA AGAGCAACAT 
TCAGAGAGCC CAGTCCATGC AGAAGCACTG GGGATGGAAG GCAGAGGGAT GCAGACCGTG 
GAGAAAGACA TGGGTACCCA AAGAGAAACA GCAGAGAGGG TGATCCCTGA GGGAGGGCCA 
CCGCAGAATG AAACCAAGAA ACTGCCCTCG GAAGGAGAGA GGGAAGATGT GACGGGAGAG 
GAAGAATTAA TCGGGGGGAT ACAGGGTAGA GAACAAAATC AGGTGTTAGC TAGAGATACT 
CAGAGCCAAG AATCTGACAA AACAGTGAAA AGTGCAAGTA CTGGAAGGGG AATGGAGATT 
GTAAAGTTAG AAACTGAGAC ACCCAAGGAA ACACAGGAGA AAGAGAGAGA AAAGCAGACT 
CTCGCAGGGG AAATATTTGA GAGTGAAGCA GGGAAACTGG TAGCAGAGAG AGAGAGTGAG 
GTAGGTGGGT TAGAAGTCAA GGGACCCCAA GAGCTACTGG ACAGAGGCCC ACAGATGAGG 
GAGACAGAGG CGGGGGGCCA GGACCAGAAA GGCCAAGCCT CTGGTCCGCC ATCAGAGCCT 
GGAGCAGGGG CAGGAGACCT TCAGGGATTT ACTTCAGATC CAGTAGCTTC TGGGAGCCAG 
GCAGGTGGAG GAAGGGGAGC CCCAGGGAGC CCCAGGAGGC AGCAGAGAGG TGACTTGAAT 
TTCGAGATGC CACCTGCTGA GAAGGCTTCC AGGGGTGATC AGGAATCCCC AGATGCTTGT 
CTGCCTCCTG CAGCGCCTGA AGCCTCAGCC GCACTCCCAA ACTCCCTCAT CTCTCAGATG 
CAAAAACATC CCGCACCTCA GTCCCTCCTT TTTCCCTCTC CAGCTCCTTT AGAACTGCCT 
ATTCCCAGGA CCAGACGAAA TGAGAGTCAG GAAGCTCCAG AGACTCCCTT CTCCTCAGAG 
CTGAACTCTG TCCACCCAGA ACCCAAAGTC AGGCCCCAGG GGTCCTCTCC AGTTTCTTCT 
CTACCCCTTG AGCCCCACCC TGCCACCCCC ACAGGCCAGC CTATTGCCCT TGAGCCCACA 
TCTGGGGTCT CTGGGAGCGG GACACATAGT TCCTTTGATG TAACTGCCTC ATCAGTTGTC 
CCCACAGCCC TTGCACTGCA GCCATCCACC TCCACAGACC AGCCTGTCAC CCCTAAGCCC 
ACACTTGGGG CCCCTCGGGG CAGGGCACAG AGGTCTTCTG TCAAAACCCC TGAACCCAGT 
GTCCGCATAG ACCAGCCTAT TGCCCCTGAG CTCACAGCTA AGGCCACTCG GGGCAGGGCA 
CAGAGGTCTT CTGTCAAGAC TCCCAAACCA GATAACTCCA CAACACCCAA GCCCCAGCCT 
TCCACTTCCA CAGACCAGCC TGTCACCCCC AAGCCCACAT CTCAGGCCCC TCGGGGCAGG 
ACACCTAAGT CTTCTGCCAA GACTCCTGAA CCAGCTGTCC CCACAGCCTC TGAGCTCCAG 
CCTGCTGCCC CTAAAGACCA GCCTGTTGCT CCTGAGCTCA CATCTAGAGC CACTCGGGGC 
AGGACACAGA GGTCTTCTAT CAAGACTTCC AAACCAGATA CGTCCACAGC CCCGAAGCCC 
CAGCCTTCCA CTTCCACAGA CCAGCCTGTC ACCCCCAAAC CCACGTCTCG GGCCTCTCGA 
GGCAGGACAC CTAGGTCTTC CACCAAGACT CCTGAACCAG TTGTCTCCAC AGCCTCTGAG 
CTCCAGCCTT CTGCCCTCAC AGACCAGCCT ATCACTCCTG AGCTCACATC TAGGGCTACT 
CGGGGCAGGG CACAGAGATC CTCTGTCAAA ACCCCTGATC CAGTTACCAC CACAACACCT 
GAGCTCCAGC CTTCCACCTC CACAGACCAG CTTGTCACCC CCAAACGCCC ATCTCGGGCC 
CCTCGAGGCA GGACACGTAG GTCGTCTGCC AAGACTCCTG AACCAGTTGT TCCCACAGCC 
TCTGAGCTCC AGCCTTCTGC CCCTGCAGAC CAGCCTGTTG GTCCTTGGGC CACTCAGTGT 
AGAAGACATA GGTCTTCTGT CAAGACCCCG GAACCAGTTG TCCCCACAGT CCCTGAACCT 
CAGCCTTCTA CTTCTAAAGA CCAGTCTGTC GCTCCTGAAC CCACATCTCA GGCCACTCAG 
AGCCAAACAC ATAGGTCTTC TGTCAAGACA TCCCAGCCAA CTGAACCCAC AGCCCCTGAC 
CTCAAACCTT CCTCCCCCAC AGACCAGCCT GTCACTCCCA AGGTCATAGC TCAGGGTGGT 
CCAAGCAGGA CACGAAGGGC TTCTACAGCA AGTGCTGTGC TGGTTCCTAC TACCCCTGAA 
TTCCAGTCTC CAGTCCGCTC AGAACAGCCT CTTTCCCCTG ACCCCATCCC CGAAGTCAAC 
TGCAGCCTGA GGCCGAGGGC CACTAGGAGA CATGGGTCTC CCACAGCTCA TGTTCATGAG 
CCCTGCACCA CACCCCCTGA ACCTAACTCT CGCTCCTCAA GGAACCAAAC ACATGGGGCA 
ATGAAGGCAG CCAAGCCCCT TAGCACCATT TCTGAGCCTG CCTTTGCCCA GCTCCCTGAG 
GCACCGCCTC ACACTCCCCA GATGCCAAAG GAGGAGGCAG CTGATGGGTC AGGCTTCACC 
CCAGAGCCCC AGCCTAGGGC CTCTCAAAAC CGCAAGAGGC CTTCAGCTAC TGCACATTCA 
CCTCCACTTC AAAAACGGCT CCAGAGAGGG AGAGTCCCTC AGAAGGCAGC ATCCCTTAAG 
GAAGAAGAAA ATCCAGCAGT AAGGCCGAGG AAGGAAGAGG GTGTAGTGAT TCCAGGTCCA 
GGCAAGAGAA GGAGAGAGCA GACAGAGGAA GAGTCCCAGG GAAGACCGAG CCGTAGCCTG 
CGACGGACCA AACCTGTCCA GGAGTCCACG GCCCCCAAAG TGCTCTTCAC GGGCGTGGTG 
GATGCTCGCG GAGAGAGGAC GGTGCTGGCC CTGGGGGGCA GTCTGGCCAG CTCAGTGGCT 
GAGGCTTCTC ACCTGGTGAC TGATCGGATC CGCCGTACGG TCAAGTTTCT GTGTGCCCTG 
GGCCGGGGCA TCCCCATCCT CTCCCTGGCC TGGCTGCATG AGTCCCGCAA GGCAGGCTGC 
TTCTTGCCCC CGGACGAATA TTTGGTGACT GATCCTGAGC AGGAGAAGAA CTTCGGCTTC 
AGCCTTCGGG AGGCCCTGAG CCGAGCTCGG GAGCGAAGGC TGCTGGAGGG CTATGAGATT 
CACGTGACCC CCGGAGTCCA GCCACCGCCA CCTCAGATGG GAGAAATCAT CAACTGCTGT 
GGAGGCGCCA TCCTGCCCAG CATGCCTCGG TCCTACAAGC CTCAGAGGGT CGTGATCACA 
TGTTCCCAGG ACTTCCCTCG ATGTGCCGTT CCATATCGGG TTGGGCTGCC TGTCCTCTCA 
CCCGAGTTCC TGCTGACGGG AGTACTGAAG CAGGAAGTCA AGCCAGAGGC CTTTGCCTTC 
TCCACTGTGG AAATGTCATC CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MDC1
Entrez Gene ID	511168
Full Name	mediator of DNA damage checkpoint 1
General protein information	Preferred Names mediator of DNA damage checkpoint 1 Names mediator of DNA damage checkpoint protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	MDC1	NP_001108419.1
Pan troglodytes (chimpanzee)	MDC1	NP_001035841.1
Canis lupus familiaris (dog)	MDC1	XP_005627164.1
Homo sapiens (human)	MDC1	NP_055456.2
Bos taurus (cattle)	MDC1	XP_002697401.2
Mus musculus (house mouse)	Mdc1	NP_001010833.2
Rattus norvegicus (Norway rat)	Mdc1	NP_001159747.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MDC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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