DOT1L cDNA ORF clone, Bos taurus(cattle)

The following DOT1L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DOT1L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa236579 XM_010807073.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa236571 XM_005209068.4
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa236571 XM_010807066.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa236572 XM_010807067.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa236573 XM_024995041.1
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa236574 XM_010807068.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa236575 XM_010807071.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236576 XM_010807072.3
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236577 XM_003586273.5
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa236578 XM_024995042.1
Latest version!
Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa90289 XM_015472118.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa90289 XM_015464181.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa21246 XM_015464182.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa21246 XM_003586273.4
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa47733 XM_015464180.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa47733 XM_010807072.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OBa05346 XM_010798345.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa05346 XM_010807067.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21243 XM_005209068.3
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21243 XM_010807066.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21243 XM_015464176.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21243 XM_015464177.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa21244 XM_015464179.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa21244 XM_010807071.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OBa21247 XM_015464183.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa21247 XM_010807073.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
OBa47730 XM_010807068.2
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OBa47730 XM_015464178.1
Latest version!
Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa236579
    Clone ID Related Accession (Same CDS sequence) XM_010807073.3
    Accession Version XM_010807073.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807073.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAGCCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805375.1
    CDS138..4634
    Translation

    Target ORF information:

    RefSeq Version XM_010807073.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807073.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAGCCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236571
    Clone ID Related Accession (Same CDS sequence) XM_010807066.3 , XM_005209068.4
    Accession Version XM_010807066.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807066.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805368.1
    CDS76..5220
    Translation

    Target ORF information:

    RefSeq Version XM_010807066.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807066.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236571
    Clone ID Related Accession (Same CDS sequence) XM_010807066.3 , XM_005209068.4
    Accession Version XM_005209068.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209068.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209125.1
    CDS76..5220
    Translation

    Target ORF information:

    RefSeq Version XM_005209068.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209068.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236572
    Clone ID Related Accession (Same CDS sequence) XM_010807067.3
    Accession Version XM_010807067.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807067.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805369.1
    CDS76..5217
    Translation

    Target ORF information:

    RefSeq Version XM_010807067.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807067.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236573
    Clone ID Related Accession (Same CDS sequence) XM_024995041.1
    Accession Version XM_024995041.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5094bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGCC GTCATGCCCC AGACTCGGCT GACAAACACC ACGATGCTGC TCATGAAATC 
    ATCGAGACCA TCAGATGGGT CTGTGAAGAA ATCCCGGATC TCAAGCTTGC TATGGAGAAT
    TACGTTTTAA TTGACTACGA TACCAAAAGC TTTGAGAGTA TGCAGAGGCT CTGTGACAAG
    TACAACCGGG CCATCGACAG CATCCACCAG CTGTGGAAGG GCACCACACA GCCCATGAAG
    CTGAACACAC GGCCATCCAC GGGGCTCCTG CGGCACATCC TGCAGCAGGT CTACAACCAC
    TCAGTGACCG ACCCTGAGAA GCTCAACAAC TACGAGCCCT TCTCCCCAGA GGTGTATGGG
    GAGACCTCCT TCGACCTGGT CGCCCAGATG ATCGATGAGA TCAAGATGAC CGAAGATGAC
    CTGTTCGTGG ACCTGGGCAG TGGAGTGGGC CAGGTTGTGC TGCAGGTTGC CGCGGCCACC
    AACTGCAAAC ATCACTACGG TGTAGAGAAA GCTGACATCC CAGCCAAGTA CGCGGAGACC
    ATGGACCGAG AGTTCAGGAA GTGGATGAAA TGGTATGGAA AAAAGCATGC AGAATACACA
    CTGGAGAGGG GGGACTTCCT CTCGGAGGAG TGGAGAGAAC GGATTGCTAA CACAAGTGTT
    GTATTTGTGA ATAACTTTGC CTTTGGTCCT GAGGTGGATC ACCAGCTGAA GGAGCGGTTT
    GCAAACATGA AGGAAGGTGG CAGAATCGTG TCCTCGAAGC CCTTTGCGCC TCTGAACTTC
    AGAATAAACA GTAGAAACTT GAGTGACATC GGCACCATCA TGCGCGTCGT GGAGCTGTCG
    CCGCTAAAAG GCTCGGTGTC ATGGACGGGG AAGCCAGTCT CCTACTACCT GCACACCATC
    GACCGCACCA TACTTGAAAA CTATTTTTCT AGTCTAAAAA ATCCAAAACT CAGGGAGGAA
    CAAGAGGCAG CTCGGCGCCG GCAGCAGCGG GAGAACAAGA GCAACACAAC CACGCCCACC
    AAGGTGCCCG AGAGCAAGGC AGCTGTGCCG GCGGACACCC CCGTGGACTC TGGTGCTGAG
    GAGGAGAAAG CCGGGACGGC CACTGTCAAG AAGCCATCTC CCTCCAAGGC CCGGAAGAAG
    AAGCTGAACA AGAAGGGCCG GAAGATGGTG GGTCGGAAGC GTGGGCGCCC CAAGAAGATG
    AGTGCCACCA ACCCCGAGCG CAAGCCCAAG AAGACCCAGA GCTCACTGGA CCTGCTGCAC
    GCCCAGACCG CGTCACGAGC GGCCTCCCCC TTGCCGCAGG ACGCGCACAG GCCACCTCAC
    AGCCCATTCT ACCAGCTACC TCCCAGCGTG CAGCGGCCTA CCCCCGAGCA GCTGCTGCTG
    GCACCCACCC CGCCCGCACT GCACAGGCTG CTAGAGTCCT TCAGGATTCA GTACCTACAG
    TTCCTGGCAT ACACGAAGAC CCCTCAGTAC AAGGCCAGCC TGCAGCAGCT CCTGGACCAG
    GAGAAGGAGA AGAATGCCCG GTTGCTAGGC GCAGCGCAGC AGCTATTTGG CCACTGCCAA
    GCTCAGAAGG AGGAGATCAA GAGGCTCTTC CAGCAGAAAC TGGACGAGCT GGGAGTGAAG
    GCGCTGACCT ACAACGACCT GATCCAAGCG CAGAAGGAGA TCTCGGCTCA CAACCAGCAG
    TTGAGGGAGC AGACGGAGCA GCTGGAGAAG GGCAACTGGG AGCTGAGGAG CCAGAGCCTG
    AGGCTGCTCA AGGCACGATG CGAGGAGCTG AAGCTGGACT GGTCCACGCT GTCGCTGGAG
    AATCTGCTGA AGGAGAAGCA GGCCCTGAAG AGCCAGATCT CAGAGAAGCA GAGGCACTGC
    CTGGAGCTGC AGATCAGCAT CGTGGAGCTG GAGAAGAGCC AGCGGCAGCA GGAGTTGCTG
    CAGCTCAAGT CCTGCGTGCC GCCTGATGAG GCACTGGCTC TGCAGCTTCG TGGGAAGCCA
    GAGGCTGAGC CTAGCCGGCT GCACTTGGAG CTGGACTGCG CCCGCTTCTC CCTGCCACCC
    TTCAGCAGCT TGAGCCCCGA GCTCTCTATG AATGGCCACG CAGCCGGCTA TGAGCTCTGC
    AGCGCACTGG GTCGGCCCTC GCCCAAGCAG AACACCCCCC AGTACCTGGC CTCCCCGCTA
    GACCAGGAGG TCGTGCCCTG CACCCCTAGC CACAGCGGCC GGCCACGGCT AGAGAAGCTG
    TCCAGCCTGG CCCTGCCTGA CTACACCAGG CTATCCCCTG CCAAGCTGGT GCTGCGGCGC
    CACCTGAGCC AGGACCACGC GGCCAGTGGC AAGGCAGCTG CCAGTGAGCT GCACCCACGA
    GCCGAGCATG CCAAGGAGAA TGGGCTCCCT TACCAGAGCC CTGGCATCAC TAATGGCATC
    AAGCTGAGCC CACAAGACCC CCGACCTTCA TCCCCCACGG CTTTACAGAT GGGAGAGAAG
    GGCCCCGAGA AGGGTGTGAA GGAGCGTGCC TACGCCAGCA GTGGGGAGGC CATCACCAGC
    CTGCCCGTCA GCATCCCGCT GAGCACCGTG CAGCCCAGCA AGCTGCCCGT CAGCATCCCC
    CTGGCCAGCG TGGTGCTGCC CAGCCGCGCC GAGAAGGCGA GAAGCACACC CAGCCCTGGG
    CCACCGGCCC GAGAGTCCTC ATCCACGCTG GAGAAGCAGG TGGCTGCTAA CACCCATGGT
    GCCGGGGGCA ACGCTGCTGG GAGCAAGAGC CTCGCCCTGG CTCCCACAGG CTTTGCATAC
    GCCGGCTCGG TGGCCATCAG TGGGGCCCTG GCCGGCAGCC CGGCACCCCT CGCCCCTGGA
    GCCGAGCCCC CAGCCCTGGA CGAGTCCTCC AGCTCTGGAA GCCTCTTTGC CACCGTGGGG
    TCCCGCAGCT CCACCCCGCA GCACCCACCC CTGCTGACGC AGCCACGCAC CTGTGGCTCG
    GCCTCGCCAG CCCCCCAGCT CTGCTCCAGC CCCCGGCTTG GCGCTCTGGG CCCACTCCCC
    GACTCTGGCA AAGGGGACCT GCCCTGCGAG GCCAGCTTCT CAGACCCTGA GAGCGAAGCC
    AAAAGGAGAA TCGTCTTCAC CATCTCGGCG GGCGCCAGCA GCGCCAAGCA GTCACCTTCC
    AGCAAACACA GCCCTCTGCC GTCGGGTGCC CGCGGGGACG GTGGCCAGGG CCATGGGCCA
    GACGGCCGCA AGCGGGGCCG GAGGAAGCGG GCATCGGCAG GGACCCCCAG CCTGAGCTCG
    AGTGTGTCCC CCAAGCGCCG GGCCTTGCCA TCTGTCGCTG GGCTTTTCAC GCAGTCTTCA
    GGGTCCCCCC TGAACCTCAA CTCCATGGTC AGCAACATCA ACCAGCCCCT GGAGATCACA
    GCAATCTCGT CCCCTGAGAG CTCCCTAAAG AGCTCACCCG TCCCCTACCA GGACAACGAC
    CAACCACCCG TGCTCAAGAA GGAGAAGCCC CTGAGCCAGA CCAATGGTGC CCACTACTCC
    CCACTGACTT CGGACGAGGA GCCAGGCTCT GAGGATGAGC CCGGCAGTGC CAGAATTGAG
    AGAAAAATTG CAACGATCTC CTTAGAAAGC AAGTCTCCGC CAAAAACCTT GGAAAATGCA
    GGTGGCAGCC TAGCAGGAAG GAAGGCGCCG CTGGCCAGCG AGCCGGTCAA CAGCAGCAAG
    TGGAAGTCCA CCTTCTCACC CATCTCCGAC CTGGGCCTGG CCAAGGCGGC CGACAGCCCG
    CTGCAGGCTG CCTCTGCTCT GAGCCACAAC TCTCTGTTCG CTTTCCGGCC TGGCCTGGAG
    GAGCCCAGTG CGGCCGATGC CAAGCTGGCC ACCCACTCCA GGAAGAGCTT CCCGGGCACC
    CTGCCAGGGG CGGGCGGGCT GAGCCCCAGT AGCCTTCCTG CCAGCGGCTT CGCCCTGGGC
    GGGGGCCTAG CAGCTGACCT CAGTTTACAC AGCTTCAGTG ATGGTGCTTC TCTCTCCCAC
    AAGGCCCCCG AGGCAGCTGG CCTGGGTGCC CCCCTGAGCT TCCCCGGCCC ACGGGGCAAA
    GAGGGTGGCG CTGCAGAGTC TGGCCCCTTC GTGAACAAGC GGCAGCTGGA CGGACTGGGC
    CCAAAAGGCG AGGGGGGCCT TCCCACATGC GGACCCCCGG ACAAGGCCTC CACAGCGCAT
    AGCAAGGCAG GCAAGGGCCG CGAGCGCGAG CCTGACGTCA AGAACGGCCA CAACCTGTTC
    ATGGCCGCTG CCACTGCGCC CCCTGCAGGC CTCCTCAGCG GCCCAGGTCT TGCCCCAGCG
    GCATCCTCGG CAGGTGGCGC AGCCCCGTCC GTGCAGACCC ACCGCCCCTT CCTGGGCGCC
    TTCGCCCCCG GGCCACAGTT CGCACTGGGC CCCATGTCGC TACAGGCCAA CCTGGGCCCA
    TCTGTGCTGC AGTCCCTGTT CAGTTCCGTG CCCGCCGCCG GCCTGGTTCA CGTCTCGACC
    GCCGCCACCA GATTGACCAA CTCGCACGCC ATGGGCAGCT TCTCCTCTGG GGTGGCCGGC
    GGCGCAGTTG GAGGTGTCTT TAACCACGCG GTGCCTCCCG CCTCCGCCCA TCCGTTTGGA
    GCCAGTTTCG GCAGTGGGGC TGCATGTCGC AGCGCCACGC TGAGCTTAAC CCCGCTGCAG
    GCGGTGGCCA GCACCCCGGC CTCTTCCTTT CAGGCCCCGT CCTCTGCGGA GCCGAGGCCA
    CCCCCACCCC CTCCGCACCT GGGCCGGCCC CCTCCGGGGC AGCCCGCCCT CCACGCACCC
    CCTCATCCTA ACGCCACCTT GCCTCCTCCC CCTGCGCTGC TCCCAGCTAA CTCTGAGCCC
    GTGCTTCTGC AGAACCTCGT ATCCCTCCCT GCTAACCAAG CTTTCTTACC CGCCTCCTCT
    GCCGCCTCTC TGCCGCCTGC TAACGCCTCT CTGTCCATCA AGCTCGCCTC CCTCCCGCAC
    AAGGTGTCCC GGCCCTCCTT GACGGTGCAC CACCAGCCCC TGCCTGGGCT GGCCCTGGCC
    CAGGCCGCGC CCGTGAACCC ACAGGCCAGC TCCACGGGGC CGCCCGCCGT GTGGGTTTCC
    CTTGGCATGC CGCCTCCGTA TGCCGCGCGC CTTGCGGGGG TTAAGCCGCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850809.1
    CDS161..5254
    Translation

    Target ORF information:

    RefSeq Version XM_024995041.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995041.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGCTGAGCC GTCATGCCCC AGACTCGGCT GACAAACACC ACGATGCTGC TCATGAAATC 
    ATCGAGACCA TCAGATGGGT CTGTGAAGAA ATCCCGGATC TCAAGCTTGC TATGGAGAAT
    TACGTTTTAA TTGACTACGA TACCAAAAGC TTTGAGAGTA TGCAGAGGCT CTGTGACAAG
    TACAACCGGG CCATCGACAG CATCCACCAG CTGTGGAAGG GCACCACACA GCCCATGAAG
    CTGAACACAC GGCCATCCAC GGGGCTCCTG CGGCACATCC TGCAGCAGGT CTACAACCAC
    TCAGTGACCG ACCCTGAGAA GCTCAACAAC TACGAGCCCT TCTCCCCAGA GGTGTATGGG
    GAGACCTCCT TCGACCTGGT CGCCCAGATG ATCGATGAGA TCAAGATGAC CGAAGATGAC
    CTGTTCGTGG ACCTGGGCAG TGGAGTGGGC CAGGTTGTGC TGCAGGTTGC CGCGGCCACC
    AACTGCAAAC ATCACTACGG TGTAGAGAAA GCTGACATCC CAGCCAAGTA CGCGGAGACC
    ATGGACCGAG AGTTCAGGAA GTGGATGAAA TGGTATGGAA AAAAGCATGC AGAATACACA
    CTGGAGAGGG GGGACTTCCT CTCGGAGGAG TGGAGAGAAC GGATTGCTAA CACAAGTGTT
    GTATTTGTGA ATAACTTTGC CTTTGGTCCT GAGGTGGATC ACCAGCTGAA GGAGCGGTTT
    GCAAACATGA AGGAAGGTGG CAGAATCGTG TCCTCGAAGC CCTTTGCGCC TCTGAACTTC
    AGAATAAACA GTAGAAACTT GAGTGACATC GGCACCATCA TGCGCGTCGT GGAGCTGTCG
    CCGCTAAAAG GCTCGGTGTC ATGGACGGGG AAGCCAGTCT CCTACTACCT GCACACCATC
    GACCGCACCA TACTTGAAAA CTATTTTTCT AGTCTAAAAA ATCCAAAACT CAGGGAGGAA
    CAAGAGGCAG CTCGGCGCCG GCAGCAGCGG GAGAACAAGA GCAACACAAC CACGCCCACC
    AAGGTGCCCG AGAGCAAGGC AGCTGTGCCG GCGGACACCC CCGTGGACTC TGGTGCTGAG
    GAGGAGAAAG CCGGGACGGC CACTGTCAAG AAGCCATCTC CCTCCAAGGC CCGGAAGAAG
    AAGCTGAACA AGAAGGGCCG GAAGATGGTG GGTCGGAAGC GTGGGCGCCC CAAGAAGATG
    AGTGCCACCA ACCCCGAGCG CAAGCCCAAG AAGACCCAGA GCTCACTGGA CCTGCTGCAC
    GCCCAGACCG CGTCACGAGC GGCCTCCCCC TTGCCGCAGG ACGCGCACAG GCCACCTCAC
    AGCCCATTCT ACCAGCTACC TCCCAGCGTG CAGCGGCCTA CCCCCGAGCA GCTGCTGCTG
    GCACCCACCC CGCCCGCACT GCACAGGCTG CTAGAGTCCT TCAGGATTCA GTACCTACAG
    TTCCTGGCAT ACACGAAGAC CCCTCAGTAC AAGGCCAGCC TGCAGCAGCT CCTGGACCAG
    GAGAAGGAGA AGAATGCCCG GTTGCTAGGC GCAGCGCAGC AGCTATTTGG CCACTGCCAA
    GCTCAGAAGG AGGAGATCAA GAGGCTCTTC CAGCAGAAAC TGGACGAGCT GGGAGTGAAG
    GCGCTGACCT ACAACGACCT GATCCAAGCG CAGAAGGAGA TCTCGGCTCA CAACCAGCAG
    TTGAGGGAGC AGACGGAGCA GCTGGAGAAG GGCAACTGGG AGCTGAGGAG CCAGAGCCTG
    AGGCTGCTCA AGGCACGATG CGAGGAGCTG AAGCTGGACT GGTCCACGCT GTCGCTGGAG
    AATCTGCTGA AGGAGAAGCA GGCCCTGAAG AGCCAGATCT CAGAGAAGCA GAGGCACTGC
    CTGGAGCTGC AGATCAGCAT CGTGGAGCTG GAGAAGAGCC AGCGGCAGCA GGAGTTGCTG
    CAGCTCAAGT CCTGCGTGCC GCCTGATGAG GCACTGGCTC TGCAGCTTCG TGGGAAGCCA
    GAGGCTGAGC CTAGCCGGCT GCACTTGGAG CTGGACTGCG CCCGCTTCTC CCTGCCACCC
    TTCAGCAGCT TGAGCCCCGA GCTCTCTATG AATGGCCACG CAGCCGGCTA TGAGCTCTGC
    AGCGCACTGG GTCGGCCCTC GCCCAAGCAG AACACCCCCC AGTACCTGGC CTCCCCGCTA
    GACCAGGAGG TCGTGCCCTG CACCCCTAGC CACAGCGGCC GGCCACGGCT AGAGAAGCTG
    TCCAGCCTGG CCCTGCCTGA CTACACCAGG CTATCCCCTG CCAAGCTGGT GCTGCGGCGC
    CACCTGAGCC AGGACCACGC GGCCAGTGGC AAGGCAGCTG CCAGTGAGCT GCACCCACGA
    GCCGAGCATG CCAAGGAGAA TGGGCTCCCT TACCAGAGCC CTGGCATCAC TAATGGCATC
    AAGCTGAGCC CACAAGACCC CCGACCTTCA TCCCCCACGG CTTTACAGAT GGGAGAGAAG
    GGCCCCGAGA AGGGTGTGAA GGAGCGTGCC TACGCCAGCA GTGGGGAGGC CATCACCAGC
    CTGCCCGTCA GCATCCCGCT GAGCACCGTG CAGCCCAGCA AGCTGCCCGT CAGCATCCCC
    CTGGCCAGCG TGGTGCTGCC CAGCCGCGCC GAGAAGGCGA GAAGCACACC CAGCCCTGGG
    CCACCGGCCC GAGAGTCCTC ATCCACGCTG GAGAAGCAGG TGGCTGCTAA CACCCATGGT
    GCCGGGGGCA ACGCTGCTGG GAGCAAGAGC CTCGCCCTGG CTCCCACAGG CTTTGCATAC
    GCCGGCTCGG TGGCCATCAG TGGGGCCCTG GCCGGCAGCC CGGCACCCCT CGCCCCTGGA
    GCCGAGCCCC CAGCCCTGGA CGAGTCCTCC AGCTCTGGAA GCCTCTTTGC CACCGTGGGG
    TCCCGCAGCT CCACCCCGCA GCACCCACCC CTGCTGACGC AGCCACGCAC CTGTGGCTCG
    GCCTCGCCAG CCCCCCAGCT CTGCTCCAGC CCCCGGCTTG GCGCTCTGGG CCCACTCCCC
    GACTCTGGCA AAGGGGACCT GCCCTGCGAG GCCAGCTTCT CAGACCCTGA GAGCGAAGCC
    AAAAGGAGAA TCGTCTTCAC CATCTCGGCG GGCGCCAGCA GCGCCAAGCA GTCACCTTCC
    AGCAAACACA GCCCTCTGCC GTCGGGTGCC CGCGGGGACG GTGGCCAGGG CCATGGGCCA
    GACGGCCGCA AGCGGGGCCG GAGGAAGCGG GCATCGGCAG GGACCCCCAG CCTGAGCTCG
    AGTGTGTCCC CCAAGCGCCG GGCCTTGCCA TCTGTCGCTG GGCTTTTCAC GCAGTCTTCA
    GGGTCCCCCC TGAACCTCAA CTCCATGGTC AGCAACATCA ACCAGCCCCT GGAGATCACA
    GCAATCTCGT CCCCTGAGAG CTCCCTAAAG AGCTCACCCG TCCCCTACCA GGACAACGAC
    CAACCACCCG TGCTCAAGAA GGAGAAGCCC CTGAGCCAGA CCAATGGTGC CCACTACTCC
    CCACTGACTT CGGACGAGGA GCCAGGCTCT GAGGATGAGC CCGGCAGTGC CAGAATTGAG
    AGAAAAATTG CAACGATCTC CTTAGAAAGC AAGTCTCCGC CAAAAACCTT GGAAAATGCA
    GGTGGCAGCC TAGCAGGAAG GAAGGCGCCG CTGGCCAGCG AGCCGGTCAA CAGCAGCAAG
    TGGAAGTCCA CCTTCTCACC CATCTCCGAC CTGGGCCTGG CCAAGGCGGC CGACAGCCCG
    CTGCAGGCTG CCTCTGCTCT GAGCCACAAC TCTCTGTTCG CTTTCCGGCC TGGCCTGGAG
    GAGCCCAGTG CGGCCGATGC CAAGCTGGCC ACCCACTCCA GGAAGAGCTT CCCGGGCACC
    CTGCCAGGGG CGGGCGGGCT GAGCCCCAGT AGCCTTCCTG CCAGCGGCTT CGCCCTGGGC
    GGGGGCCTAG CAGCTGACCT CAGTTTACAC AGCTTCAGTG ATGGTGCTTC TCTCTCCCAC
    AAGGCCCCCG AGGCAGCTGG CCTGGGTGCC CCCCTGAGCT TCCCCGGCCC ACGGGGCAAA
    GAGGGTGGCG CTGCAGAGTC TGGCCCCTTC GTGAACAAGC GGCAGCTGGA CGGACTGGGC
    CCAAAAGGCG AGGGGGGCCT TCCCACATGC GGACCCCCGG ACAAGGCCTC CACAGCGCAT
    AGCAAGGCAG GCAAGGGCCG CGAGCGCGAG CCTGACGTCA AGAACGGCCA CAACCTGTTC
    ATGGCCGCTG CCACTGCGCC CCCTGCAGGC CTCCTCAGCG GCCCAGGTCT TGCCCCAGCG
    GCATCCTCGG CAGGTGGCGC AGCCCCGTCC GTGCAGACCC ACCGCCCCTT CCTGGGCGCC
    TTCGCCCCCG GGCCACAGTT CGCACTGGGC CCCATGTCGC TACAGGCCAA CCTGGGCCCA
    TCTGTGCTGC AGTCCCTGTT CAGTTCCGTG CCCGCCGCCG GCCTGGTTCA CGTCTCGACC
    GCCGCCACCA GATTGACCAA CTCGCACGCC ATGGGCAGCT TCTCCTCTGG GGTGGCCGGC
    GGCGCAGTTG GAGGTGTCTT TAACCACGCG GTGCCTCCCG CCTCCGCCCA TCCGTTTGGA
    GCCAGTTTCG GCAGTGGGGC TGCATGTCGC AGCGCCACGC TGAGCTTAAC CCCGCTGCAG
    GCGGTGGCCA GCACCCCGGC CTCTTCCTTT CAGGCCCCGT CCTCTGCGGA GCCGAGGCCA
    CCCCCACCCC CTCCGCACCT GGGCCGGCCC CCTCCGGGGC AGCCCGCCCT CCACGCACCC
    CCTCATCCTA ACGCCACCTT GCCTCCTCCC CCTGCGCTGC TCCCAGCTAA CTCTGAGCCC
    GTGCTTCTGC AGAACCTCGT ATCCCTCCCT GCTAACCAAG CTTTCTTACC CGCCTCCTCT
    GCCGCCTCTC TGCCGCCTGC TAACGCCTCT CTGTCCATCA AGCTCGCCTC CCTCCCGCAC
    AAGGTGTCCC GGCCCTCCTT GACGGTGCAC CACCAGCCCC TGCCTGGGCT GGCCCTGGCC
    CAGGCCGCGC CCGTGAACCC ACAGGCCAGC TCCACGGGGC CGCCCGCCGT GTGGGTTTCC
    CTTGGCATGC CGCCTCCGTA TGCCGCGCGC CTTGCGGGGG TTAAGCCGCG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236574
    Clone ID Related Accession (Same CDS sequence) XM_010807068.3
    Accession Version XM_010807068.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAGC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805370.1
    CDS76..5157
    Translation

    Target ORF information:

    RefSeq Version XM_010807068.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807068.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAGC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236575
    Clone ID Related Accession (Same CDS sequence) XM_010807071.3
    Accession Version XM_010807071.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807071.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAG CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805373.1
    CDS173..5155
    Translation

    Target ORF information:

    RefSeq Version XM_010807071.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807071.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAG CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236576
    Clone ID Related Accession (Same CDS sequence) XM_010807072.3
    Accession Version XM_010807072.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807072.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805374.1
    CDS76..4860
    Translation

    Target ORF information:

    RefSeq Version XM_010807072.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807072.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236577
    Clone ID Related Accession (Same CDS sequence) XM_003586273.5
    Accession Version XM_003586273.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_003586273.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586321.2
    CDS76..4647
    Translation

    Target ORF information:

    RefSeq Version XM_003586273.5
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586273.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236578
    Clone ID Related Accession (Same CDS sequence) XM_024995042.1
    Accession Version XM_024995042.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4569bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024850810.1
    CDS76..4644
    Translation

    Target ORF information:

    RefSeq Version XM_024995042.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1 like histone lysine methyltransferase (DOT1L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995042.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AGCCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90289
    Clone ID Related Accession (Same CDS sequence) XM_015464181.1 , XM_015472118.1
    Accession Version XM_015464181.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG
    GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC
    AGGTTCCCTT CCAGGGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319667.1
    CDS65..4768
    Translation

    Target ORF information:

    RefSeq Version XM_015464181.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464181.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG
    GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC
    AGGTTCCCTT CCAGGGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90289
    Clone ID Related Accession (Same CDS sequence) XM_015464181.1 , XM_015472118.1
    Accession Version XM_015472118.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4704bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG
    GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC
    AGGTTCCCTT CCAGGGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015327604.1
    CDS65..4768
    Translation

    Target ORF information:

    RefSeq Version XM_015472118.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015472118.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAGGC AGTGCCCTCC TGGCCACTTG GTCCCACAGG ACCCGCTGAG GGACGAGCTG
    GGGGCCGCAC GTTGTCTCCG CCACCCACCC AGACTGTTGG GTTTAACCAC CGCTGTTTGC
    AGGTTCCCTT CCAGGGCCCC CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21246
    Clone ID Related Accession (Same CDS sequence) XM_003586273.4 , XM_015464182.1
    Accession Version XM_003586273.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_003586273.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003586321.2
    CDS64..4635
    Translation

    Target ORF information:

    RefSeq Version XM_003586273.4
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003586273.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21246
    Clone ID Related Accession (Same CDS sequence) XM_003586273.4 , XM_015464182.1
    Accession Version XM_015464182.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4572bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319668.1
    CDS64..4635
    Translation

    Target ORF information:

    RefSeq Version XM_015464182.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464182.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47733
    Clone ID Related Accession (Same CDS sequence) XM_015464180.1 , XM_010807072.2
    Accession Version XM_015464180.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319666.1
    CDS65..4849
    Translation

    Target ORF information:

    RefSeq Version XM_015464180.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464180.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47733
    Clone ID Related Accession (Same CDS sequence) XM_015464180.1 , XM_010807072.2
    Accession Version XM_010807072.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807072.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805374.1
    CDS65..4849
    Translation

    Target ORF information:

    RefSeq Version XM_010807072.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807072.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTTCCC TTCCAGGGCC CCCTGAGGGG GGAGAGGCTG TGCCCGCGTG TCCCTTTGGG
    TCCACTTTGC GCCCGCCACC TCGTTCCTTT TCACGAAACG CAGCCGTGAC GGTCGTGTGG
    GCAGAGCACT CGGGCGTGTC TTTAACCACG CGGTGCCTCC CGCCTCCGCC CATCCGTTTG
    GAGCCAGTTT CGGCAGTGGG GCTGCATGTC GCAGCGCCAC GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05346
    Clone ID Related Accession (Same CDS sequence) XM_010798345.2 , XM_010807067.2
    Accession Version XM_010798345.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010798345.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010796647.1
    CDS67..5208
    Translation

    Target ORF information:

    RefSeq Version XM_010798345.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010798345.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa05346
    Clone ID Related Accession (Same CDS sequence) XM_010798345.2 , XM_010807067.2
    Accession Version XM_010807067.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805369.1
    CDS67..5208
    Translation

    Target ORF information:

    RefSeq Version XM_010807067.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807067.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21243
    Clone ID Related Accession (Same CDS sequence) XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
    Accession Version XM_015464176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319662.1
    CDS67..5211
    Translation

    Target ORF information:

    RefSeq Version XM_015464176.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21243
    Clone ID Related Accession (Same CDS sequence) XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
    Accession Version XM_005209068.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209068.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209125.1
    CDS67..5211
    Translation

    Target ORF information:

    RefSeq Version XM_005209068.3
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209068.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21243
    Clone ID Related Accession (Same CDS sequence) XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
    Accession Version XM_010807066.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807066.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805368.1
    CDS67..5211
    Translation

    Target ORF information:

    RefSeq Version XM_010807066.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807066.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21243
    Clone ID Related Accession (Same CDS sequence) XM_015464176.1 , XM_005209068.3 , XM_010807066.2 , XM_015464177.1
    Accession Version XM_015464177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5145bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319663.1
    CDS67..5211
    Translation

    Target ORF information:

    RefSeq Version XM_015464177.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGAC CATGGACCGA
    GAGTTCAGGA AGTGGATGAA ATGGTATGGA AAAAAGCATG CAGAATACAC ACTGGAGAGG
    GGGGACTTCC TCTCGGAGGA GTGGAGAGAA CGGATTGCTA ACACAAGTGT TGTATTTGTG
    AATAACTTTG CCTTTGGTCC TGAGGTGGAT CACCAGCTGA AGGAGCGGTT TGCAAACATG
    AAGGAAGGTG GCAGAATCGT GTCCTCGAAG CCCTTTGCGC CTCTGAACTT CAGAATAAAC
    AGTAGAAACT TGAGTGACAT CGGCACCATC ATGCGCGTCG TGGAGCTGTC GCCGCTAAAA
    GGCTCGGTGT CATGGACGGG GAAGCCAGTC TCCTACTACC TGCACACCAT CGACCGCACC
    ATACTTGAAA ACTATTTTTC TAGTCTAAAA AATCCAAAAC TCAGGGAGGA ACAAGAGGCA
    GCTCGGCGCC GGCAGCAGCG GGAGAACAAG AGCAACACAA CCACGCCCAC CAAGGTGCCC
    GAGAGCAAGG CAGCTGTGCC GGCGGACACC CCCGTGGACT CTGGTGCTGA GGAGGAGAAA
    GCCGGGACGG CCACTGTCAA GAAGCCATCT CCCTCCAAGG CCCGGAAGAA GAAGCTGAAC
    AAGAAGGGCC GGAAGATGGT GGGTCGGAAG CGTGGGCGCC CCAAGAAGAT GAGTGCCACC
    AACCCCGAGC GCAAGCCCAA GAAGACCCAG AGCTCACTGG ACCTGCTGCA CGCCCAGACC
    GCGTCACGAG CGGCCTCCCC CTTGCCGCAG GACGCGCACA GGCCACCTCA CAGCCCATTC
    TACCAGCTAC CTCCCAGCGT GCAGCGGCCT ACCCCCGAGC AGCTGCTGCT GGCACCCACC
    CCGCCCGCAC TGCACAGGCT GCTAGAGTCC TTCAGGATTC AGTACCTACA GTTCCTGGCA
    TACACGAAGA CCCCTCAGTA CAAGGCCAGC CTGCAGCAGC TCCTGGACCA GGAGAAGGAG
    AAGAATGCCC GGTTGCTAGG CGCAGCGCAG CAGCTATTTG GCCACTGCCA AGCTCAGAAG
    GAGGAGATCA AGAGGCTCTT CCAGCAGAAA CTGGACGAGC TGGGAGTGAA GGCGCTGACC
    TACAACGACC TGATCCAAGC GCAGAAGGAG ATCTCGGCTC ACAACCAGCA GTTGAGGGAG
    CAGACGGAGC AGCTGGAGAA GGGCAACTGG GAGCTGAGGA GCCAGAGCCT GAGGCTGCTC
    AAGGCACGAT GCGAGGAGCT GAAGCTGGAC TGGTCCACGC TGTCGCTGGA GAATCTGCTG
    AAGGAGAAGC AGGCCCTGAA GAGCCAGATC TCAGAGAAGC AGAGGCACTG CCTGGAGCTG
    CAGATCAGCA TCGTGGAGCT GGAGAAGAGC CAGCGGCAGC AGGAGTTGCT GCAGCTCAAG
    TCCTGCGTGC CGCCTGATGA GGCACTGGCT CTGCAGCTTC GTGGGAAGCC AGAGGCTGAG
    CCTAGCCGGC TGCACTTGGA GCTGGACTGC GCCCGCTTCT CCCTGCCACC CTTCAGCAGC
    TTGAGCCCCG AGCTCTCTAT GAATGGCCAC GCAGCCGGCT ATGAGCTCTG CAGCGCACTG
    GGTCGGCCCT CGCCCAAGCA GAACACCCCC CAGTACCTGG CCTCCCCGCT AGACCAGGAG
    GTCGTGCCCT GCACCCCTAG CCACAGCGGC CGGCCACGGC TAGAGAAGCT GTCCAGCCTG
    GCCCTGCCTG ACTACACCAG GCTATCCCCT GCCAAGCTGG TGCTGCGGCG CCACCTGAGC
    CAGGACCACG CGGCCAGTGG CAAGGCAGCT GCCAGTGAGC TGCACCCACG AGCCGAGCAT
    GCCAAGGAGA ATGGGCTCCC TTACCAGAGC CCTGGCATCA CTAATGGCAT CAAGCTGAGC
    CCACAAGACC CCCGACCTTC ATCCCCCACG GCTTTACAGA TGGGAGAGAA GGGCCCCGAG
    AAGGGTGTGA AGGAGCGTGC CTACGCCAGC AGTGGGGAGG CCATCACCAG CCTGCCCGTC
    AGCATCCCGC TGAGCACCGT GCAGCCCAGC AAGCTGCCCG TCAGCATCCC CCTGGCCAGC
    GTGGTGCTGC CCAGCCGCGC CGAGAAGGCG AGAAGCACAC CCAGCCCTGG GCCACCGGCC
    CGAGAGTCCT CATCCACGCT GGAGAAGCAG GTGGCTGCTA ACACCCATGG TGCCGGGGGC
    AACGCTGCTG GGAGCAAGAG CCTCGCCCTG GCTCCCACAG GCTTTGCATA CGCCGGCTCG
    GTGGCCATCA GTGGGGCCCT GGCCGGCAGC CCGGCACCCC TCGCCCCTGG AGCCGAGCCC
    CCAGCCCTGG ACGAGTCCTC CAGCTCTGGA AGCCTCTTTG CCACCGTGGG GTCCCGCAGC
    TCCACCCCGC AGCACCCACC CCTGCTGACG CAGCCACGCA CCTGTGGCTC GGCCTCGCCA
    GCCCCCCAGC TCTGCTCCAG CCCCCGGCTT GGCGCTCTGG GCCCACTCCC CGACTCTGGC
    AAAGGGGACC TGCCCTGCGA GGCCAGCTTC TCAGACCCTG AGAGCGAAGC CAAAAGGAGA
    ATCGTCTTCA CCATCTCGGC GGGCGCCAGC AGCGCCAAGC AGTCACCTTC CAGCAAACAC
    AGCCCTCTGC CGTCGGGTGC CCGCGGGGAC GGTGGCCAGG GCCATGGGCC AGACGGCCGC
    AAGCGGGGCC GGAGGAAGCG GGCATCGGCA GGGACCCCCA GCCTGAGCTC GAGTGTGTCC
    CCCAAGCGCC GGGCCTTGCC ATCTGTCGCT GGGCTTTTCA CGCAGTCTTC AGGGTCCCCC
    CTGAACCTCA ACTCCATGGT CAGCAACATC AACCAGCCCC TGGAGATCAC AGCAATCTCG
    TCCCCTGAGA GCTCCCTAAA GAGCTCACCC GTCCCCTACC AGGACAACGA CCAACCACCC
    GTGCTCAAGA AGGAGAAGCC CCTGAGCCAG ACCAATGGTG CCCACTACTC CCCACTGACT
    TCGGACGAGG AACCAGGCTC TGAGGATGAG CCCGGCAGTG CCAGAATTGA GAGAAAAATT
    GCAACGATCT CCTTAGAAAG CAAGTCTCCG CCAAAAACCT TGGAAAATGC AGGTGGCAGC
    CTAGCAGGAA GGAAGGCGCC GCTGGCCAGC GAGCCGGTCA ACAGCAGCAA GTGGAAGTCC
    ACCTTCTCAC CCATCTCCGA CCTGGGCCTG GCCAAGGCGG CCGACAGCCC GCTGCAGGCT
    GCCTCTGCTC TGAGCCACAA CTCTCTGTTC GCTTTCCGGC CTGGCCTGGA GGAGCCCAGT
    GCGGCCGATG CCAAGCTGGC CACCCACTCC AGGAAGAGCT TCCCGGGCAC CCTGCCAGGG
    GCGGGCGGGC TGAGCCCCAG TAGCCTTCCT GCCAGCGGCT TCGCCCTGGG CGGGGGCCTA
    GCAGCTGACC TCAGTTTACA CAGCTTCAGT GATGGTGCTT CTCTCTCCCA CAAGGCCCCC
    GAGGCAGCTG GCCTGGGTGC CCCCCTGAGC TTCCCCGGCC CACGGGGCAA AGAGGGTGGC
    GCTGCAGAGT CTGGCCCCTT CGTGAACAAG CGGCAGCTGG ACGGACTGGG CCCAAAAGGC
    GAGGGGGGCC TTCCCACATG CGGACCCCCG GACAAGGCCT CCACAGCGCA TAGCAAGGCA
    GGCAAGGGCC GCGAGCGCGA GCCTGACGTC AAGAACGGCC ACAACCTGTT CATGGCCGCT
    GCCACTGCGC CCCCTGCAGG CCTCCTCAGC GGCCCAGGTC TTGCCCCAGC GGCATCCTCG
    GCAGGTGGCG CAGCCCCGTC CGTGCAGACC CACCGCCCCT TCCTGGGCGC CTTCGCCCCC
    GGGCCACAGT TCGCACTGGG CCCCATGTCG CTACAGGCCA ACCTGGGCCC ATCTGTGCTG
    CAGTCCCTGT TCAGTTCCGT GCCCGCCGCC GGCCTGGTTC ACGTCTCGAC CGCCGCCACC
    AGATTGACCA ACTCGCACGC CATGGGCAGC TTCTCCTCTG GGGTGGCCGG CGGCGCAGTT
    GGAGGTGTCT TTAACCACGC GGTGCCTCCC GCCTCCGCCC ATCCGTTTGG AGCCAGTTTC
    GGCAGTGGGG CTGCATGTCG CAGCGCCACG CTGAGCTTAA CCCCGCTGCA GGCGGTGGCC
    AGCACCCCGG CCTCTTCCTT TCAGGCCCCG TCCTCTGCGG AGCCGAGGCC ACCCCCACCC
    CCTCCGCACC TGGGCCGGCC CCCTCCGGGG CAGCCCGCCC TCCACGCACC CCCTCATCCT
    AACGCCACCT TGCCTCCTCC CCCTGCGCTG CTCCCAGCTA ACTCTGAGCC CGTGCTTCTG
    CAGAACCTCG TATCCCTCCC TGCTAACCAA GCTTTCTTAC CCGCCTCCTC TGCCGCCTCT
    CTGCCGCCTG CTAACGCCTC TCTGTCCATC AAGCTCGCCT CCCTCCCGCA CAAGGTGTCC
    CGGCCCTCCT TGACGGTGCA CCACCAGCCC CTGCCTGGGC TGGCCCTGGC CCAGGCCGCG
    CCCGTGAACC CACAGGCCAG CTCCACGGGG CCGCCCGCCG TGTGGGTTTC CCTTGGCATG
    CCGCCTCCGT ATGCCGCGCG CCTTGCGGGG GTTAAGCCGC GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21244
    Clone ID Related Accession (Same CDS sequence) XM_010807071.2 , XM_015464179.1
    Accession Version XM_010807071.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807071.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805373.1
    CDS154..5136
    Translation

    Target ORF information:

    RefSeq Version XM_010807071.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807071.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21244
    Clone ID Related Accession (Same CDS sequence) XM_010807071.2 , XM_015464179.1
    Accession Version XM_015464179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319665.1
    CDS154..5136
    Translation

    Target ORF information:

    RefSeq Version XM_015464179.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGGAGAATT ACGTTTTAAT TGACTACGAT ACCAAAAGCT TTGAGAGTAT GCAGAGGCTC 
    TGTGACAAGT ACAACCGGGC CATCGACAGC ATCCACCAGC TGTGGAAGGG CACCACACAG
    CCCATGAAGC TGAACACACG GCCATCCACG GGGCTCCTGC GGCACATCCT GCAGCAGGTC
    TACAACCACT CAGTGACCGA CCCTGAGAAG CTCAACAACT ACGAGCCCTT CTCCCCAGAG
    GTGTATGGGG AGACCTCCTT CGACCTGGTC GCCCAGATGA TCGATGAGAT CAAGATGACC
    GAAGATGACC TGTTCGTGGA CCTGGGCAGT GGAGTGGGCC AGGTTGTGCT GCAGGTTGCC
    GCGGCCACCA ACTGCAAACA TCACTACGGT GTAGAGAAAG CTGACATCCC AGCCAAGTAC
    GCGGAGACCA TGGACCGAGA GTTCAGGAAG TGGATGAAAT GGTATGGAAA AAAGCATGCA
    GAATACACAC TGGAGAGGGG GGACTTCCTC TCGGAGGAGT GGAGAGAACG GATTGCTAAC
    ACAAGTGTTG TATTTGTGAA TAACTTTGCC TTTGGTCCTG AGGTGGATCA CCAGCTGAAG
    GAGCGGTTTG CAAACATGAA GGAAGGTGGC AGAATCGTGT CCTCGAAGCC CTTTGCGCCT
    CTGAACTTCA GAATAAACAG TAGAAACTTG AGTGACATCG GCACCATCAT GCGCGTCGTG
    GAGCTGTCGC CGCTAAAAGG CTCGGTGTCA TGGACGGGGA AGCCAGTCTC CTACTACCTG
    CACACCATCG ACCGCACCAT ACTTGAAAAC TATTTTTCTA GTCTAAAAAA TCCAAAACTC
    AGGGAGGAAC AAGAGGCAGC TCGGCGCCGG CAGCAGCGGG AGAACAAGAG CAACACAACC
    ACGCCCACCA AGGTGCCCGA GAGCAAGGCA GCTGTGCCGG CGGACACCCC CGTGGACTCT
    GGTGCTGAGG AGGAGAAAGC CGGGACGGCC ACTGTCAAGA AGCCATCTCC CTCCAAGGCC
    CGGAAGAAGA AGCTGAACAA GAAGGGCCGG AAGATGGTGG GTCGGAAGCG TGGGCGCCCC
    AAGAAGATGA GTGCCACCAA CCCCGAGCGC AAGCCCAAGA AGACCCAGAG CTCACTGGAC
    CTGCTGCACG CCCAGACCGC GTCACGAGCG GCCTCCCCCT TGCCGCAGGA CGCGCACAGG
    CCACCTCACA GCCCATTCTA CCAGCTACCT CCCAGCGTGC AGCGGCCTAC CCCCGAGCAG
    CTGCTGCTGG CACCCACCCC GCCCGCACTG CACAGGCTGC TAGAGTCCTT CAGGATTCAG
    TACCTACAGT TCCTGGCATA CACGAAGACC CCTCAGTACA AGGCCAGCCT GCAGCAGCTC
    CTGGACCAGG AGAAGGAGAA GAATGCCCGG TTGCTAGGCG CAGCGCAGCA GCTATTTGGC
    CACTGCCAAG CTCAGAAGGA GGAGATCAAG AGGCTCTTCC AGCAGAAACT GGACGAGCTG
    GGAGTGAAGG CGCTGACCTA CAACGACCTG ATCCAAGCGC AGAAGGAGAT CTCGGCTCAC
    AACCAGCAGT TGAGGGAGCA GACGGAGCAG CTGGAGAAGG GCAACTGGGA GCTGAGGAGC
    CAGAGCCTGA GGCTGCTCAA GGCACGATGC GAGGAGCTGA AGCTGGACTG GTCCACGCTG
    TCGCTGGAGA ATCTGCTGAA GGAGAAGCAG GCCCTGAAGA GCCAGATCTC AGAGAAGCAG
    AGGCACTGCC TGGAGCTGCA GATCAGCATC GTGGAGCTGG AGAAGAGCCA GCGGCAGCAG
    GAGTTGCTGC AGCTCAAGTC CTGCGTGCCG CCTGATGAGG CACTGGCTCT GCAGCTTCGT
    GGGAAGCCAG AGGCTGAGCC TAGCCGGCTG CACTTGGAGC TGGACTGCGC CCGCTTCTCC
    CTGCCACCCT TCAGCAGCTT GAGCCCCGAG CTCTCTATGA ATGGCCACGC AGCCGGCTAT
    GAGCTCTGCA GCGCACTGGG TCGGCCCTCG CCCAAGCAGA ACACCCCCCA GTACCTGGCC
    TCCCCGCTAG ACCAGGAGGT CGTGCCCTGC ACCCCTAGCC ACAGCGGCCG GCCACGGCTA
    GAGAAGCTGT CCAGCCTGGC CCTGCCTGAC TACACCAGGC TATCCCCTGC CAAGCTGGTG
    CTGCGGCGCC ACCTGAGCCA GGACCACGCG GCCAGTGGCA AGGCAGCTGC CAGTGAGCTG
    CACCCACGAG CCGAGCATGC CAAGGAGAAT GGGCTCCCTT ACCAGAGCCC TGGCATCACT
    AATGGCATCA AGCTGAGCCC ACAAGACCCC CGACCTTCAT CCCCCACGGC TTTACAGATG
    GGAGAGAAGG GCCCCGAGAA GGGTGTGAAG GAGCGTGCCT ACGCCAGCAG TGGGGAGGCC
    ATCACCAGCC TGCCCGTCAG CATCCCGCTG AGCACCGTGC AGCCCAGCAA GCTGCCCGTC
    AGCATCCCCC TGGCCAGCGT GGTGCTGCCC AGCCGCGCCG AGAAGGCGAG AAGCACACCC
    AGCCCTGGGC CACCGGCCCG AGAGTCCTCA TCCACGCTGG AGAAGCAGGT GGCTGCTAAC
    ACCCATGGTG CCGGGGGCAA CGCTGCTGGG AGCAAGAGCC TCGCCCTGGC TCCCACAGGC
    TTTGCATACG CCGGCTCGGT GGCCATCAGT GGGGCCCTGG CCGGCAGCCC GGCACCCCTC
    GCCCCTGGAG CCGAGCCCCC AGCCCTGGAC GAGTCCTCCA GCTCTGGAAG CCTCTTTGCC
    ACCGTGGGGT CCCGCAGCTC CACCCCGCAG CACCCACCCC TGCTGACGCA GCCACGCACC
    TGTGGCTCGG CCTCGCCAGC CCCCCAGCTC TGCTCCAGCC CCCGGCTTGG CGCTCTGGGC
    CCACTCCCCG ACTCTGGCAA AGGGGACCTG CCCTGCGAGG CCAGCTTCTC AGACCCTGAG
    AGCGAAGCCA AAAGGAGAAT CGTCTTCACC ATCTCGGCGG GCGCCAGCAG CGCCAAGCAG
    TCACCTTCCA GCAAACACAG CCCTCTGCCG TCGGGTGCCC GCGGGGACGG TGGCCAGGGC
    CATGGGCCAG ACGGCCGCAA GCGGGGCCGG AGGAAGCGGG CATCGGCAGG GACCCCCAGC
    CTGAGCTCGA GTGTGTCCCC CAAGCGCCGG GCCTTGCCAT CTGTCGCTGG GCTTTTCACG
    CAGTCTTCAG GGTCCCCCCT GAACCTCAAC TCCATGGTCA GCAACATCAA CCAGCCCCTG
    GAGATCACAG CAATCTCGTC CCCTGAGAGC TCCCTAAAGA GCTCACCCGT CCCCTACCAG
    GACAACGACC AACCACCCGT GCTCAAGAAG GAGAAGCCCC TGAGCCAGAC CAATGGTGCC
    CACTACTCCC CACTGACTTC GGACGAGGAA CCAGGCTCTG AGGATGAGCC CGGCAGTGCC
    AGAATTGAGA GAAAAATTGC AACGATCTCC TTAGAAAGCA AGTCTCCGCC AAAAACCTTG
    GAAAATGCAG GTGGCAGCCT AGCAGGAAGG AAGGCGCCGC TGGCCAGCGA GCCGGTCAAC
    AGCAGCAAGT GGAAGTCCAC CTTCTCACCC ATCTCCGACC TGGGCCTGGC CAAGGCGGCC
    GACAGCCCGC TGCAGGCTGC CTCTGCTCTG AGCCACAACT CTCTGTTCGC TTTCCGGCCT
    GGCCTGGAGG AGCCCAGTGC GGCCGATGCC AAGCTGGCCA CCCACTCCAG GAAGAGCTTC
    CCGGGCACCC TGCCAGGGGC GGGCGGGCTG AGCCCCAGTA GCCTTCCTGC CAGCGGCTTC
    GCCCTGGGCG GGGGCCTAGC AGCTGACCTC AGTTTACACA GCTTCAGTGA TGGTGCTTCT
    CTCTCCCACA AGGCCCCCGA GGCAGCTGGC CTGGGTGCCC CCCTGAGCTT CCCCGGCCCA
    CGGGGCAAAG AGGGTGGCGC TGCAGAGTCT GGCCCCTTCG TGAACAAGCG GCAGCTGGAC
    GGACTGGGCC CAAAAGGCGA GGGGGGCCTT CCCACATGCG GACCCCCGGA CAAGGCCTCC
    ACAGCGCATA GCAAGGCAGG CAAGGGCCGC GAGCGCGAGC CTGACGTCAA GAACGGCCAC
    AACCTGTTCA TGGCCGCTGC CACTGCGCCC CCTGCAGGCC TCCTCAGCGG CCCAGGTCTT
    GCCCCAGCGG CATCCTCGGC AGGTGGCGCA GCCCCGTCCG TGCAGACCCA CCGCCCCTTC
    CTGGGCGCCT TCGCCCCCGG GCCACAGTTC GCACTGGGCC CCATGTCGCT ACAGGCCAAC
    CTGGGCCCAT CTGTGCTGCA GTCCCTGTTC AGTTCCGTGC CCGCCGCCGG CCTGGTTCAC
    GTCTCGACCG CCGCCACCAG ATTGACCAAC TCGCACGCCA TGGGCAGCTT CTCCTCTGGG
    GTGGCCGGCG GCGCAGTTGG AGGTGTCTTT AACCACGCGG TGCCTCCCGC CTCCGCCCAT
    CCGTTTGGAG CCAGTTTCGG CAGTGGGGCT GCATGTCGCA GCGCCACGCT GAGCTTAACC
    CCGCTGCAGG CGGTGGCCAG CACCCCGGCC TCTTCCTTTC AGGCCCCGTC CTCTGCGGAG
    CCGAGGCCAC CCCCACCCCC TCCGCACCTG GGCCGGCCCC CTCCGGGGCA GCCCGCCCTC
    CACGCACCCC CTCATCCTAA CGCCACCTTG CCTCCTCCCC CTGCGCTGCT CCCAGCTAAC
    TCTGAGCCCG TGCTTCTGCA GAACCTCGTA TCCCTCCCTG CTAACCAAGC TTTCTTACCC
    GCCTCCTCTG CCGCCTCTCT GCCGCCTGCT AACGCCTCTC TGTCCATCAA GCTCGCCTCC
    CTCCCGCACA AGGTGTCCCG GCCCTCCTTG ACGGTGCACC ACCAGCCCCT GCCTGGGCTG
    GCCCTGGCCC AGGCCGCGCC CGTGAACCCA CAGGCCAGCT CCACGGGGCC GCCCGCCGTG
    TGGGTTTCCC TTGGCATGCC GCCTCCGTAT GCCGCGCGCC TTGCGGGGGT TAAGCCGCGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21247
    Clone ID Related Accession (Same CDS sequence) XM_010807073.2 , XM_015464183.1
    Accession Version XM_010807073.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807073.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805375.1
    CDS108..4604
    Translation

    Target ORF information:

    RefSeq Version XM_010807073.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807073.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21247
    Clone ID Related Accession (Same CDS sequence) XM_010807073.2 , XM_015464183.1
    Accession Version XM_015464183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4497bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319669.1
    CDS108..4604
    Translation

    Target ORF information:

    RefSeq Version XM_015464183.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGCTGGAGA GGGGGGACTT CCTCTCGGAG GAGTGGAGAG AACGGATTGC TAACACAAGT 
    GTTGTATTTG TGAATAACTT TGCCTTTGGT CCTGAGGTGG ATCACCAGCT GAAGGAGCGG
    TTTGCAAACA TGAAGGAAGG TGGCAGAATC GTGTCCTCGA AGCCCTTTGC GCCTCTGAAC
    TTCAGAATAA ACAGTAGAAA CTTGAGTGAC ATCGGCACCA TCATGCGCGT CGTGGAGCTG
    TCGCCGCTAA AAGGCTCGGT GTCATGGACG GGGAAGCCAG TCTCCTACTA CCTGCACACC
    ATCGACCGCA CCATACTTGA AAACTATTTT TCTAGTCTAA AAAATCCAAA ACTCAGGGAG
    GAACAAGAGG CAGCTCGGCG CCGGCAGCAG CGGGAGAACA AGAGCAACAC AACCACGCCC
    ACCAAGGTGC CCGAGAGCAA GGCAGCTGTG CCGGCGGACA CCCCCGTGGA CTCTGGTGCT
    GAGGAGGAGA AAGCCGGGAC GGCCACTGTC AAGAAGCCAT CTCCCTCCAA GGCCCGGAAG
    AAGAAGCTGA ACAAGAAGGG CCGGAAGATG GTGGGTCGGA AGCGTGGGCG CCCCAAGAAG
    ATGAGTGCCA CCAACCCCGA GCGCAAGCCC AAGAAGACCC AGAGCTCACT GGACCTGCTG
    CACGCCCAGA CCGCGTCACG AGCGGCCTCC CCCTTGCCGC AGGACGCGCA CAGGCCACCT
    CACAGCCCAT TCTACCAGCT ACCTCCCAGC GTGCAGCGGC CTACCCCCGA GCAGCTGCTG
    CTGGCACCCA CCCCGCCCGC ACTGCACAGG CTGCTAGAGT CCTTCAGGAT TCAGTACCTA
    CAGTTCCTGG CATACACGAA GACCCCTCAG TACAAGGCCA GCCTGCAGCA GCTCCTGGAC
    CAGGAGAAGG AGAAGAATGC CCGGTTGCTA GGCGCAGCGC AGCAGCTATT TGGCCACTGC
    CAAGCTCAGA AGGAGGAGAT CAAGAGGCTC TTCCAGCAGA AACTGGACGA GCTGGGAGTG
    AAGGCGCTGA CCTACAACGA CCTGATCCAA GCGCAGAAGG AGATCTCGGC TCACAACCAG
    CAGTTGAGGG AGCAGACGGA GCAGCTGGAG AAGGGCAACT GGGAGCTGAG GAGCCAGAGC
    CTGAGGCTGC TCAAGGCACG ATGCGAGGAG CTGAAGCTGG ACTGGTCCAC GCTGTCGCTG
    GAGAATCTGC TGAAGGAGAA GCAGGCCCTG AAGAGCCAGA TCTCAGAGAA GCAGAGGCAC
    TGCCTGGAGC TGCAGATCAG CATCGTGGAG CTGGAGAAGA GCCAGCGGCA GCAGGAGTTG
    CTGCAGCTCA AGTCCTGCGT GCCGCCTGAT GAGGCACTGG CTCTGCAGCT TCGTGGGAAG
    CCAGAGGCTG AGCCTAGCCG GCTGCACTTG GAGCTGGACT GCGCCCGCTT CTCCCTGCCA
    CCCTTCAGCA GCTTGAGCCC CGAGCTCTCT ATGAATGGCC ACGCAGCCGG CTATGAGCTC
    TGCAGCGCAC TGGGTCGGCC CTCGCCCAAG CAGAACACCC CCCAGTACCT GGCCTCCCCG
    CTAGACCAGG AGGTCGTGCC CTGCACCCCT AGCCACAGCG GCCGGCCACG GCTAGAGAAG
    CTGTCCAGCC TGGCCCTGCC TGACTACACC AGGCTATCCC CTGCCAAGCT GGTGCTGCGG
    CGCCACCTGA GCCAGGACCA CGCGGCCAGT GGCAAGGCAG CTGCCAGTGA GCTGCACCCA
    CGAGCCGAGC ATGCCAAGGA GAATGGGCTC CCTTACCAGA GCCCTGGCAT CACTAATGGC
    ATCAAGCTGA GCCCACAAGA CCCCCGACCT TCATCCCCCA CGGCTTTACA GATGGGAGAG
    AAGGGCCCCG AGAAGGGTGT GAAGGAGCGT GCCTACGCCA GCAGTGGGGA GGCCATCACC
    AGCCTGCCCG TCAGCATCCC GCTGAGCACC GTGCAGCCCA GCAAGCTGCC CGTCAGCATC
    CCCCTGGCCA GCGTGGTGCT GCCCAGCCGC GCCGAGAAGG CGAGAAGCAC ACCCAGCCCT
    GGGCCACCGG CCCGAGAGTC CTCATCCACG CTGGAGAAGC AGGTGGCTGC TAACACCCAT
    GGTGCCGGGG GCAACGCTGC TGGGAGCAAG AGCCTCGCCC TGGCTCCCAC AGGCTTTGCA
    TACGCCGGCT CGGTGGCCAT CAGTGGGGCC CTGGCCGGCA GCCCGGCACC CCTCGCCCCT
    GGAGCCGAGC CCCCAGCCCT GGACGAGTCC TCCAGCTCTG GAAGCCTCTT TGCCACCGTG
    GGGTCCCGCA GCTCCACCCC GCAGCACCCA CCCCTGCTGA CGCAGCCACG CACCTGTGGC
    TCGGCCTCGC CAGCCCCCCA GCTCTGCTCC AGCCCCCGGC TTGGCGCTCT GGGCCCACTC
    CCCGACTCTG GCAAAGGGGA CCTGCCCTGC GAGGCCAGCT TCTCAGACCC TGAGAGCGAA
    GCCAAAAGGA GAATCGTCTT CACCATCTCG GCGGGCGCCA GCAGCGCCAA GCAGTCACCT
    TCCAGCAAAC ACAGCCCTCT GCCGTCGGGT GCCCGCGGGG ACGGTGGCCA GGGCCATGGG
    CCAGACGGCC GCAAGCGGGG CCGGAGGAAG CGGGCATCGG CAGGGACCCC CAGCCTGAGC
    TCGAGTGTGT CCCCCAAGCG CCGGGCCTTG CCATCTGTCG CTGGGCTTTT CACGCAGTCT
    TCAGGGTCCC CCCTGAACCT CAACTCCATG GTCAGCAACA TCAACCAGCC CCTGGAGATC
    ACAGCAATCT CGTCCCCTGA GAGCTCCCTA AAGAGCTCAC CCGTCCCCTA CCAGGACAAC
    GACCAACCAC CCGTGCTCAA GAAGGAGAAG CCCCTGAGCC AGACCAATGG TGCCCACTAC
    TCCCCACTGA CTTCGGACGA GGAACCAGGC TCTGAGGATG AGCCCGGCAG TGCCAGAATT
    GAGAGAAAAA TTGCAACGAT CTCCTTAGAA AGCAAGTCTC CGCCAAAAAC CTTGGAAAAT
    GCAGGTGGCA GCCTAGCAGG AAGGAAGGCG CCGCTGGCCA GCGAGCCGGT CAACAGCAGC
    AAGTGGAAGT CCACCTTCTC ACCCATCTCC GACCTGGGCC TGGCCAAGGC GGCCGACAGC
    CCGCTGCAGG CTGCCTCTGC TCTGAGCCAC AACTCTCTGT TCGCTTTCCG GCCTGGCCTG
    GAGGAGCCCA GTGCGGCCGA TGCCAAGCTG GCCACCCACT CCAGGAAGAG CTTCCCGGGC
    ACCCTGCCAG GGGCGGGCGG GCTGAGCCCC AGTAGCCTTC CTGCCAGCGG CTTCGCCCTG
    GGCGGGGGCC TAGCAGCTGA CCTCAGTTTA CACAGCTTCA GTGATGGTGC TTCTCTCTCC
    CACAAGGCCC CCGAGGCAGC TGGCCTGGGT GCCCCCCTGA GCTTCCCCGG CCCACGGGGC
    AAAGAGGGTG GCGCTGCAGA GTCTGGCCCC TTCGTGAACA AGCGGCAGCT GGACGGACTG
    GGCCCAAAAG GCGAGGGGGG CCTTCCCACA TGCGGACCCC CGGACAAGGC CTCCACAGCG
    CATAGCAAGG CAGGCAAGGG CCGCGAGCGC GAGCCTGACG TCAAGAACGG CCACAACCTG
    TTCATGGCCG CTGCCACTGC GCCCCCTGCA GGCCTCCTCA GCGGCCCAGG TCTTGCCCCA
    GCGGCATCCT CGGCAGGTGG CGCAGCCCCG TCCGTGCAGA CCCACCGCCC CTTCCTGGGC
    GCCTTCGCCC CCGGGCCACA GTTCGCACTG GGCCCCATGT CGCTACAGGC CAACCTGGGC
    CCATCTGTGC TGCAGTCCCT GTTCAGTTCC GTGCCCGCCG CCGGCCTGGT TCACGTCTCG
    ACCGCCGCCA CCAGATTGAC CAACTCGCAC GCCATGGGCA GCTTCTCCTC TGGGGTGGCC
    GGCGGCGCAG TTGGAGGTGT CTTTAACCAC GCGGTGCCTC CCGCCTCCGC CCATCCGTTT
    GGAGCCAGTT TCGGCAGTGG GGCTGCATGT CGCAGCGCCA CGCTGAGCTT AACCCCGCTG
    CAGGCGGTGG CCAGCACCCC GGCCTCTTCC TTTCAGGCCC CGTCCTCTGC GGAGCCGAGG
    CCACCCCCAC CCCCTCCGCA CCTGGGCCGG CCCCCTCCGG GGCAGCCCGC CCTCCACGCA
    CCCCCTCATC CTAACGCCAC CTTGCCTCCT CCCCCTGCGC TGCTCCCAGC TAACTCTGAG
    CCCGTGCTTC TGCAGAACCT CGTATCCCTC CCTGCTAACC AAGCTTTCTT ACCCGCCTCC
    TCTGCCGCCT CTCTGCCGCC TGCTAACGCC TCTCTGTCCA TCAAGCTCGC CTCCCTCCCG
    CACAAGGTGT CCCGGCCCTC CTTGACGGTG CACCACCAGC CCCTGCCTGG GCTGGCCCTG
    GCCCAGGCCG CGCCCGTGAA CCCACAGGCC AGCTCCACGG GGCCGCCCGC CGTGTGGGTT
    TCCCTTGGCA TGCCGCCTCC GTATGCCGCG CGCCTTGCGG GGGTTAAGCC GCGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47730
    Clone ID Related Accession (Same CDS sequence) XM_015464178.1 , XM_010807068.2
    Accession Version XM_015464178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645060.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319664.1
    CDS67..5148
    Translation

    Target ORF information:

    RefSeq Version XM_015464178.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47730
    Clone ID Related Accession (Same CDS sequence) XM_015464178.1 , XM_010807068.2
    Accession Version XM_010807068.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5082bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product histone-lysine N-methyltransferase, H3 lysine-79 specific isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104019.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807068.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805370.1
    CDS67..5148
    Translation

    Target ORF information:

    RefSeq Version XM_010807068.2
    Organism Bos taurus(cattle)
    Definition Bos taurus DOT1-like histone H3K79 methyltransferase (DOT1L), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807068.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGGGGAGA AGCTGGAGCT GAGGCTCAAG TCGCCCGTGG GGGCCGAGCC CGCCGTCTAC 
    CCGTGGCCGC TGCCGGTCTA CGACAAACAC CACGATGCTG CTCATGAAAT CATCGAGACC
    ATCAGATGGG TCTGTGAAGA AATCCCGGAT CTCAAGCTTG CTATGGAGAA TTACGTTTTA
    ATTGACTACG ATACCAAAAG CTTTGAGAGT ATGCAGAGGC TCTGTGACAA GTACAACCGG
    GCCATCGACA GCATCCACCA GCTGTGGAAG GGCACCACAC AGCCCATGAA GCTGAACACA
    CGGCCATCCA CGGGGCTCCT GCGGCACATC CTGCAGCAGG TCTACAACCA CTCAGTGACC
    GACCCTGAGA AGCTCAACAA CTACGAGCCC TTCTCCCCAG AGGTGTATGG GGAGACCTCC
    TTCGACCTGG TCGCCCAGAT GATCGATGAG ATCAAGATGA CCGAAGATGA CCTGTTCGTG
    GACCTGGGCA GTGGAGTGGG CCAGGTTGTG CTGCAGGTTG CCGCGGCCAC CAACTGCAAA
    CATCACTACG GTGTAGAGAA AGCTGACATC CCAGCCAAGT ACGCGGAGCT GGAGAGGGGG
    GACTTCCTCT CGGAGGAGTG GAGAGAACGG ATTGCTAACA CAAGTGTTGT ATTTGTGAAT
    AACTTTGCCT TTGGTCCTGA GGTGGATCAC CAGCTGAAGG AGCGGTTTGC AAACATGAAG
    GAAGGTGGCA GAATCGTGTC CTCGAAGCCC TTTGCGCCTC TGAACTTCAG AATAAACAGT
    AGAAACTTGA GTGACATCGG CACCATCATG CGCGTCGTGG AGCTGTCGCC GCTAAAAGGC
    TCGGTGTCAT GGACGGGGAA GCCAGTCTCC TACTACCTGC ACACCATCGA CCGCACCATA
    CTTGAAAACT ATTTTTCTAG TCTAAAAAAT CCAAAACTCA GGGAGGAACA AGAGGCAGCT
    CGGCGCCGGC AGCAGCGGGA GAACAAGAGC AACACAACCA CGCCCACCAA GGTGCCCGAG
    AGCAAGGCAG CTGTGCCGGC GGACACCCCC GTGGACTCTG GTGCTGAGGA GGAGAAAGCC
    GGGACGGCCA CTGTCAAGAA GCCATCTCCC TCCAAGGCCC GGAAGAAGAA GCTGAACAAG
    AAGGGCCGGA AGATGGTGGG TCGGAAGCGT GGGCGCCCCA AGAAGATGAG TGCCACCAAC
    CCCGAGCGCA AGCCCAAGAA GACCCAGAGC TCACTGGACC TGCTGCACGC CCAGACCGCG
    TCACGAGCGG CCTCCCCCTT GCCGCAGGAC GCGCACAGGC CACCTCACAG CCCATTCTAC
    CAGCTACCTC CCAGCGTGCA GCGGCCTACC CCCGAGCAGC TGCTGCTGGC ACCCACCCCG
    CCCGCACTGC ACAGGCTGCT AGAGTCCTTC AGGATTCAGT ACCTACAGTT CCTGGCATAC
    ACGAAGACCC CTCAGTACAA GGCCAGCCTG CAGCAGCTCC TGGACCAGGA GAAGGAGAAG
    AATGCCCGGT TGCTAGGCGC AGCGCAGCAG CTATTTGGCC ACTGCCAAGC TCAGAAGGAG
    GAGATCAAGA GGCTCTTCCA GCAGAAACTG GACGAGCTGG GAGTGAAGGC GCTGACCTAC
    AACGACCTGA TCCAAGCGCA GAAGGAGATC TCGGCTCACA ACCAGCAGTT GAGGGAGCAG
    ACGGAGCAGC TGGAGAAGGG CAACTGGGAG CTGAGGAGCC AGAGCCTGAG GCTGCTCAAG
    GCACGATGCG AGGAGCTGAA GCTGGACTGG TCCACGCTGT CGCTGGAGAA TCTGCTGAAG
    GAGAAGCAGG CCCTGAAGAG CCAGATCTCA GAGAAGCAGA GGCACTGCCT GGAGCTGCAG
    ATCAGCATCG TGGAGCTGGA GAAGAGCCAG CGGCAGCAGG AGTTGCTGCA GCTCAAGTCC
    TGCGTGCCGC CTGATGAGGC ACTGGCTCTG CAGCTTCGTG GGAAGCCAGA GGCTGAGCCT
    AGCCGGCTGC ACTTGGAGCT GGACTGCGCC CGCTTCTCCC TGCCACCCTT CAGCAGCTTG
    AGCCCCGAGC TCTCTATGAA TGGCCACGCA GCCGGCTATG AGCTCTGCAG CGCACTGGGT
    CGGCCCTCGC CCAAGCAGAA CACCCCCCAG TACCTGGCCT CCCCGCTAGA CCAGGAGGTC
    GTGCCCTGCA CCCCTAGCCA CAGCGGCCGG CCACGGCTAG AGAAGCTGTC CAGCCTGGCC
    CTGCCTGACT ACACCAGGCT ATCCCCTGCC AAGCTGGTGC TGCGGCGCCA CCTGAGCCAG
    GACCACGCGG CCAGTGGCAA GGCAGCTGCC AGTGAGCTGC ACCCACGAGC CGAGCATGCC
    AAGGAGAATG GGCTCCCTTA CCAGAGCCCT GGCATCACTA ATGGCATCAA GCTGAGCCCA
    CAAGACCCCC GACCTTCATC CCCCACGGCT TTACAGATGG GAGAGAAGGG CCCCGAGAAG
    GGTGTGAAGG AGCGTGCCTA CGCCAGCAGT GGGGAGGCCA TCACCAGCCT GCCCGTCAGC
    ATCCCGCTGA GCACCGTGCA GCCCAGCAAG CTGCCCGTCA GCATCCCCCT GGCCAGCGTG
    GTGCTGCCCA GCCGCGCCGA GAAGGCGAGA AGCACACCCA GCCCTGGGCC ACCGGCCCGA
    GAGTCCTCAT CCACGCTGGA GAAGCAGGTG GCTGCTAACA CCCATGGTGC CGGGGGCAAC
    GCTGCTGGGA GCAAGAGCCT CGCCCTGGCT CCCACAGGCT TTGCATACGC CGGCTCGGTG
    GCCATCAGTG GGGCCCTGGC CGGCAGCCCG GCACCCCTCG CCCCTGGAGC CGAGCCCCCA
    GCCCTGGACG AGTCCTCCAG CTCTGGAAGC CTCTTTGCCA CCGTGGGGTC CCGCAGCTCC
    ACCCCGCAGC ACCCACCCCT GCTGACGCAG CCACGCACCT GTGGCTCGGC CTCGCCAGCC
    CCCCAGCTCT GCTCCAGCCC CCGGCTTGGC GCTCTGGGCC CACTCCCCGA CTCTGGCAAA
    GGGGACCTGC CCTGCGAGGC CAGCTTCTCA GACCCTGAGA GCGAAGCCAA AAGGAGAATC
    GTCTTCACCA TCTCGGCGGG CGCCAGCAGC GCCAAGCAGT CACCTTCCAG CAAACACAGC
    CCTCTGCCGT CGGGTGCCCG CGGGGACGGT GGCCAGGGCC ATGGGCCAGA CGGCCGCAAG
    CGGGGCCGGA GGAAGCGGGC ATCGGCAGGG ACCCCCAGCC TGAGCTCGAG TGTGTCCCCC
    AAGCGCCGGG CCTTGCCATC TGTCGCTGGG CTTTTCACGC AGTCTTCAGG GTCCCCCCTG
    AACCTCAACT CCATGGTCAG CAACATCAAC CAGCCCCTGG AGATCACAGC AATCTCGTCC
    CCTGAGAGCT CCCTAAAGAG CTCACCCGTC CCCTACCAGG ACAACGACCA ACCACCCGTG
    CTCAAGAAGG AGAAGCCCCT GAGCCAGACC AATGGTGCCC ACTACTCCCC ACTGACTTCG
    GACGAGGAAC CAGGCTCTGA GGATGAGCCC GGCAGTGCCA GAATTGAGAG AAAAATTGCA
    ACGATCTCCT TAGAAAGCAA GTCTCCGCCA AAAACCTTGG AAAATGCAGG TGGCAGCCTA
    GCAGGAAGGA AGGCGCCGCT GGCCAGCGAG CCGGTCAACA GCAGCAAGTG GAAGTCCACC
    TTCTCACCCA TCTCCGACCT GGGCCTGGCC AAGGCGGCCG ACAGCCCGCT GCAGGCTGCC
    TCTGCTCTGA GCCACAACTC TCTGTTCGCT TTCCGGCCTG GCCTGGAGGA GCCCAGTGCG
    GCCGATGCCA AGCTGGCCAC CCACTCCAGG AAGAGCTTCC CGGGCACCCT GCCAGGGGCG
    GGCGGGCTGA GCCCCAGTAG CCTTCCTGCC AGCGGCTTCG CCCTGGGCGG GGGCCTAGCA
    GCTGACCTCA GTTTACACAG CTTCAGTGAT GGTGCTTCTC TCTCCCACAA GGCCCCCGAG
    GCAGCTGGCC TGGGTGCCCC CCTGAGCTTC CCCGGCCCAC GGGGCAAAGA GGGTGGCGCT
    GCAGAGTCTG GCCCCTTCGT GAACAAGCGG CAGCTGGACG GACTGGGCCC AAAAGGCGAG
    GGGGGCCTTC CCACATGCGG ACCCCCGGAC AAGGCCTCCA CAGCGCATAG CAAGGCAGGC
    AAGGGCCGCG AGCGCGAGCC TGACGTCAAG AACGGCCACA ACCTGTTCAT GGCCGCTGCC
    ACTGCGCCCC CTGCAGGCCT CCTCAGCGGC CCAGGTCTTG CCCCAGCGGC ATCCTCGGCA
    GGTGGCGCAG CCCCGTCCGT GCAGACCCAC CGCCCCTTCC TGGGCGCCTT CGCCCCCGGG
    CCACAGTTCG CACTGGGCCC CATGTCGCTA CAGGCCAACC TGGGCCCATC TGTGCTGCAG
    TCCCTGTTCA GTTCCGTGCC CGCCGCCGGC CTGGTTCACG TCTCGACCGC CGCCACCAGA
    TTGACCAACT CGCACGCCAT GGGCAGCTTC TCCTCTGGGG TGGCCGGCGG CGCAGTTGGA
    GGTGTCTTTA ACCACGCGGT GCCTCCCGCC TCCGCCCATC CGTTTGGAGC CAGTTTCGGC
    AGTGGGGCTG CATGTCGCAG CGCCACGCTG AGCTTAACCC CGCTGCAGGC GGTGGCCAGC
    ACCCCGGCCT CTTCCTTTCA GGCCCCGTCC TCTGCGGAGC CGAGGCCACC CCCACCCCCT
    CCGCACCTGG GCCGGCCCCC TCCGGGGCAG CCCGCCCTCC ACGCACCCCC TCATCCTAAC
    GCCACCTTGC CTCCTCCCCC TGCGCTGCTC CCAGCTAACT CTGAGCCCGT GCTTCTGCAG
    AACCTCGTAT CCCTCCCTGC TAACCAAGCT TTCTTACCCG CCTCCTCTGC CGCCTCTCTG
    CCGCCTGCTA ACGCCTCTCT GTCCATCAAG CTCGCCTCCC TCCCGCACAA GGTGTCCCGG
    CCCTCCTTGA CGGTGCACCA CCAGCCCCTG CCTGGGCTGG CCCTGGCCCA GGCCGCGCCC
    GTGAACCCAC AGGCCAGCTC CACGGGGCCG CCCGCCGTGT GGGTTTCCCT TGGCATGCCG
    CCTCCGTATG CCGCGCGCCT TGCGGGGGTT AAGCCGCGAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.