IARS cDNA ORF clone, Bos taurus(cattle)

The following IARS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IARS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa237006 XM_024995578.1
Latest version!
Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa40687 NM_001101069.2
Latest version!
Bos taurus isoleucyl-tRNA synthetase 1 (IARS1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa22284 XM_005210426.4
Latest version!
Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa22284 XM_005210426.3 Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa237006
    Clone ID Related Accession (Same CDS sequence) XM_024995578.1
    Accession Version XM_024995578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGATACT CTACTGAATG GAAGTCTACT ATTACCAGAC TTGGCCGATG GATTGACTTT 
    GACAATGACT ACAAAACTCT GTATCCACAA TTCATGGAAT CTGTTTGGTG GATCTTCAAA
    CAACTCTATG ATAAAGGCCT TGTTTATAGA GGTGTGAAAG TCATGCCCTT CTCCACTGCA
    TGCAATACTC CACTCTCCAA CTTTGAATCT CACCAGAATT ACAAGGATGT TCAAGATCCT
    TCAATATTTG TAACTTTCCC TTTGGAGGAA GATGAAAATA TTTCTTTGGT TGCCTGGACA
    ACTACTCCCT GGACTCTTCC TAGTAACCTT GCCCTGTGTG TTAATCCCGA CTTGCAATAT
    GTGAAGATTA AAGATTCTGT CAGAGGAAAA TTACTCATTT TAATGGAAGC CAGATTATCA
    GCCCTCTATA AATCGGAGAG TGACTATGAG ATCCTTGAAA GATTTCCTGG TGCCTCTCTC
    AGAGGCAAGA AATACCGGCC CCTGTTTGAC TATTTTGTAA AGTGTAAAGA GAGTGGTGCT
    TTTACTGTGC TTGTGGACAA CTATGTGAAA GAGGAAGAAG GCACTGGGAT TGTACACCAA
    GCTCCTTACT TTGGTGCTGA CGATTATCGG GTCTGTATGG ATTTCAACAT CATTCAGAAA
    GACTCTCTCC CTATTTGCCC TGTGGATGCC TCAGGCTGTT TTACAGCTGA AGTGACAGAT
    TTTGCAGGAC AGTATGTGAA GGATGCTGAC AAAAATATCA TCAAGACTCT AAAGGAACAA
    GGTCGACTTC TTGTTGCCAG CACCTTCACC CATAGCTACC CTTTTTGTTG GAGGTCAGAC
    ACTCCACTCA TTTACAAAGC AGTGCCTAGC TGGTTCGTTA GGGTGGAGCA CATGGTGGAT
    CAGCTGCTGA GGAACAATGA CCTGTGTTAC TGGGTCCCAG AGTTTGTGCG AGAAAAGCGA
    TTTGGAAATT GGCTGAAAGA TGCACGTGAC TGGGCAATTT CCAGAAACAG ATACTGGGGC
    ACTCCTATCC CACTGTGGGT CAGCAGTGAC TTCGAGGAGG TAGTATGTAT TGGGTCAATA
    GCAGAACTGG AAGAACTATC AGGAACAAAG ATCTCAGATC TCCACAGAGA GAGCATTGAC
    CATCTGACCA TTCCTTCACG CTGTGGAAAG GGATTGTTGC ATCGTGTCTC TGAAGTGTTT
    GACTGTTGGT TCGAGAGTGG CAGCATGCCA TATGCTCAAA TTCATTATCC ATTTGAAAAC
    AAGAGGGAGT TTGAGGATGC ATTTCCTGCA GACTTCATTG CTGAAGGCAT TGACCAAACC
    AGAGGATGGT TTTACACCTT GCTGGTGCTA GCCACATCTC TCTTTGGACG GCCACCATTC
    AAAAATGTGA TTGTGAATGG ACTTGTCCTG GCAAGTGATG GTCAAAAAAT GAGCAAACGC
    AAAAAGAATT ATCCAGACCC ACTTTCAGTA ATTGATAAAT ATGGTGCTGA TGCCCTCAGA
    TTATATCTGA TTAACTCCCC TGTGGTGAGA GCAGAAAACC TTCGCTTTAA GGAAGAGGGT
    GTCCGAGATG TCCTGAAGGA TGTACTGCTC CCTTGGTACA ATGCCTATCG CTTCTTCATC
    CAGAACGTCC TAAGGCTACA GAAGGAAGAA GAAGTGGAAT TCCTTTACAA CGAGAACATG
    GTTAAAGAAA GTACCAACAT CACAGACCGG TGGGTCCTGT CCTTTATGCA GTCGCTCGTT
    GGGTTCTTTG AGACCGAAAT GGCAGCTTAC AGGCTCTATA CTGTGGTTCC TCGCCTGGTC
    AAGTTTGTGG ATATTTTGAC CAATTGGTAT GTTCGGATGA ACCGTAGAAG ATTAAAGGGT
    GAAAATGGAG TAGAGGATTG TGTTAAGGCC CTGGAGACCT TGTTTAGTGT TCTGCTGTCT
    TTGTGCAGAC TTATGGCCCC CTATACCCCT TTTCTTACTG AATTGATGTA TCAGAATCTG
    AAGACACTGA TTGACCCTGT TTCTGTCCAG GACAAGGACA CATTCAGTAT TCACTACCTC
    ATGCTGCCCC ATGTTCGTGA GGAATTGATC GACAAGAAAA CTGAGAGTGC AGTATCTAGA
    ATGCAGTCTG TGATTGAACT TGGCCGAGTG ATTCGAGACC GCAAAACTAT TCCAATAAAG
    TATCCTTTGA AAGAAATTGT TGTTATCCAT CAAGATCAGG AAGCTCTTAA TGAAGTTAAG
    TCTTTGGAGA AATATATCAT TGAGGAATTG AATGTTCGAA AAGTTACATT ATCTACAGAT
    AAAAACAAGT ATGGCGTTCG CCTAAGGGCA GAGCCAGATC ACATGGTCCT GGGGAAGCGT
    CTGAAAGGAG CTTTCAAGAT GGTAATGACA TCCATCAAAC AGCTGAGCAA TGAGGAGCTG
    GAGCGGTTCC AGGAAAATGG GACCATTGTT GTGGAAGGCC ATGAATTGCA TGAAGAAGAT
    ATCCGTCTCA TATACACCTT TGATCAGGCA ACAGGTGGAA CAACACAGTT TGAAGCACAC
    TCGGATGCCC AGGTTTTGGT TCTCTTAGAT GTCACCCCTG ACCAGTCAAT GGTGGATGAA
    GGAATGGCTC GGGAAGTCAT CAATCGTATC CAGAAACTTC GCAAAAAGTG TAATCTGGTT
    CCAACTGATG AAATAACAGT TTATTATAAA GCAACTTCTG AGGGAAAATA TCTGAATAAT
    GTTATCGAAA GCCACACAGA CTTCATATTT GCTACCATAA AGTCTCCTTT GAAACCATAT
    CCAGTTCCTA TATCGGATGA AATACTTATT CAAGAAAAAA TGCAGTTAAA AGGGTCTGAC
    CTGGAAATTA CACTCACCAA AGGATCTTCC ACGCATGGCC CTGCTTGTGC ATATGTCAAT
    CTTAACATTT GTACAAATGG CAGTGAGCAA GGTGGAGTAT TACTTCTGGA AAATCCAAAA
    GGAGATAATA GGTTGGACCT TGTAAAACTG AAGAATGTTG TTACTAGCAT TTTTGGTGTA
    AAAAATACAG AGCTGGCTGT CCTCCTTGGT GAAACAGAAA TAAAAAACCA AACCAACTTA
    CTGAATCTTA GTGGGAAAAC ACTGTGTGTG ACTGCAGGAT CAGCGCTGTC TGTGGTGGAC
    AGTCCTAGTA CTCTTCTCTG TCAGTACATC AACCTACAGC TCTTGAATGC AGATCCACAA
    GAGTGTTTAA TGGGAACAGT AGGCACTCTT CTGCTTGAAA ATCCACTTGG ACAGAATGGG
    CTTACCCACC AAGGTCTTCT GTATGAAGCT GCCAAGATGT TTGGCCTCCG GAGCAGGAAG
    CTAAAGCTGT TTCTGAATGA GACTCAAACA GAAGAAATTA CAGAAGAAAT CCCTATGAAG
    ACTTTGAATA CGAAGACTGT GTATGTTTCT GTGTTACCAA CTACAGCAGA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851346.1
    CDS394..3810
    Translation

    Target ORF information:

    RefSeq Version XM_024995578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGCGATACT CTACTGAATG GAAGTCTACT ATTACCAGAC TTGGCCGATG GATTGACTTT 
    GACAATGACT ACAAAACTCT GTATCCACAA TTCATGGAAT CTGTTTGGTG GATCTTCAAA
    CAACTCTATG ATAAAGGCCT TGTTTATAGA GGTGTGAAAG TCATGCCCTT CTCCACTGCA
    TGCAATACTC CACTCTCCAA CTTTGAATCT CACCAGAATT ACAAGGATGT TCAAGATCCT
    TCAATATTTG TAACTTTCCC TTTGGAGGAA GATGAAAATA TTTCTTTGGT TGCCTGGACA
    ACTACTCCCT GGACTCTTCC TAGTAACCTT GCCCTGTGTG TTAATCCCGA CTTGCAATAT
    GTGAAGATTA AAGATTCTGT CAGAGGAAAA TTACTCATTT TAATGGAAGC CAGATTATCA
    GCCCTCTATA AATCGGAGAG TGACTATGAG ATCCTTGAAA GATTTCCTGG TGCCTCTCTC
    AGAGGCAAGA AATACCGGCC CCTGTTTGAC TATTTTGTAA AGTGTAAAGA GAGTGGTGCT
    TTTACTGTGC TTGTGGACAA CTATGTGAAA GAGGAAGAAG GCACTGGGAT TGTACACCAA
    GCTCCTTACT TTGGTGCTGA CGATTATCGG GTCTGTATGG ATTTCAACAT CATTCAGAAA
    GACTCTCTCC CTATTTGCCC TGTGGATGCC TCAGGCTGTT TTACAGCTGA AGTGACAGAT
    TTTGCAGGAC AGTATGTGAA GGATGCTGAC AAAAATATCA TCAAGACTCT AAAGGAACAA
    GGTCGACTTC TTGTTGCCAG CACCTTCACC CATAGCTACC CTTTTTGTTG GAGGTCAGAC
    ACTCCACTCA TTTACAAAGC AGTGCCTAGC TGGTTCGTTA GGGTGGAGCA CATGGTGGAT
    CAGCTGCTGA GGAACAATGA CCTGTGTTAC TGGGTCCCAG AGTTTGTGCG AGAAAAGCGA
    TTTGGAAATT GGCTGAAAGA TGCACGTGAC TGGGCAATTT CCAGAAACAG ATACTGGGGC
    ACTCCTATCC CACTGTGGGT CAGCAGTGAC TTCGAGGAGG TAGTATGTAT TGGGTCAATA
    GCAGAACTGG AAGAACTATC AGGAACAAAG ATCTCAGATC TCCACAGAGA GAGCATTGAC
    CATCTGACCA TTCCTTCACG CTGTGGAAAG GGATTGTTGC ATCGTGTCTC TGAAGTGTTT
    GACTGTTGGT TCGAGAGTGG CAGCATGCCA TATGCTCAAA TTCATTATCC ATTTGAAAAC
    AAGAGGGAGT TTGAGGATGC ATTTCCTGCA GACTTCATTG CTGAAGGCAT TGACCAAACC
    AGAGGATGGT TTTACACCTT GCTGGTGCTA GCCACATCTC TCTTTGGACG GCCACCATTC
    AAAAATGTGA TTGTGAATGG ACTTGTCCTG GCAAGTGATG GTCAAAAAAT GAGCAAACGC
    AAAAAGAATT ATCCAGACCC ACTTTCAGTA ATTGATAAAT ATGGTGCTGA TGCCCTCAGA
    TTATATCTGA TTAACTCCCC TGTGGTGAGA GCAGAAAACC TTCGCTTTAA GGAAGAGGGT
    GTCCGAGATG TCCTGAAGGA TGTACTGCTC CCTTGGTACA ATGCCTATCG CTTCTTCATC
    CAGAACGTCC TAAGGCTACA GAAGGAAGAA GAAGTGGAAT TCCTTTACAA CGAGAACATG
    GTTAAAGAAA GTACCAACAT CACAGACCGG TGGGTCCTGT CCTTTATGCA GTCGCTCGTT
    GGGTTCTTTG AGACCGAAAT GGCAGCTTAC AGGCTCTATA CTGTGGTTCC TCGCCTGGTC
    AAGTTTGTGG ATATTTTGAC CAATTGGTAT GTTCGGATGA ACCGTAGAAG ATTAAAGGGT
    GAAAATGGAG TAGAGGATTG TGTTAAGGCC CTGGAGACCT TGTTTAGTGT TCTGCTGTCT
    TTGTGCAGAC TTATGGCCCC CTATACCCCT TTTCTTACTG AATTGATGTA TCAGAATCTG
    AAGACACTGA TTGACCCTGT TTCTGTCCAG GACAAGGACA CATTCAGTAT TCACTACCTC
    ATGCTGCCCC ATGTTCGTGA GGAATTGATC GACAAGAAAA CTGAGAGTGC AGTATCTAGA
    ATGCAGTCTG TGATTGAACT TGGCCGAGTG ATTCGAGACC GCAAAACTAT TCCAATAAAG
    TATCCTTTGA AAGAAATTGT TGTTATCCAT CAAGATCAGG AAGCTCTTAA TGAAGTTAAG
    TCTTTGGAGA AATATATCAT TGAGGAATTG AATGTTCGAA AAGTTACATT ATCTACAGAT
    AAAAACAAGT ATGGCGTTCG CCTAAGGGCA GAGCCAGATC ACATGGTCCT GGGGAAGCGT
    CTGAAAGGAG CTTTCAAGAT GGTAATGACA TCCATCAAAC AGCTGAGCAA TGAGGAGCTG
    GAGCGGTTCC AGGAAAATGG GACCATTGTT GTGGAAGGCC ATGAATTGCA TGAAGAAGAT
    ATCCGTCTCA TATACACCTT TGATCAGGCA ACAGGTGGAA CAACACAGTT TGAAGCACAC
    TCGGATGCCC AGGTTTTGGT TCTCTTAGAT GTCACCCCTG ACCAGTCAAT GGTGGATGAA
    GGAATGGCTC GGGAAGTCAT CAATCGTATC CAGAAACTTC GCAAAAAGTG TAATCTGGTT
    CCAACTGATG AAATAACAGT TTATTATAAA GCAACTTCTG AGGGAAAATA TCTGAATAAT
    GTTATCGAAA GCCACACAGA CTTCATATTT GCTACCATAA AGTCTCCTTT GAAACCATAT
    CCAGTTCCTA TATCGGATGA AATACTTATT CAAGAAAAAA TGCAGTTAAA AGGGTCTGAC
    CTGGAAATTA CACTCACCAA AGGATCTTCC ACGCATGGCC CTGCTTGTGC ATATGTCAAT
    CTTAACATTT GTACAAATGG CAGTGAGCAA GGTGGAGTAT TACTTCTGGA AAATCCAAAA
    GGAGATAATA GGTTGGACCT TGTAAAACTG AAGAATGTTG TTACTAGCAT TTTTGGTGTA
    AAAAATACAG AGCTGGCTGT CCTCCTTGGT GAAACAGAAA TAAAAAACCA AACCAACTTA
    CTGAATCTTA GTGGGAAAAC ACTGTGTGTG ACTGCAGGAT CAGCGCTGTC TGTGGTGGAC
    AGTCCTAGTA CTCTTCTCTG TCAGTACATC AACCTACAGC TCTTGAATGC AGATCCACAA
    GAGTGTTTAA TGGGAACAGT AGGCACTCTT CTGCTTGAAA ATCCACTTGG ACAGAATGGG
    CTTACCCACC AAGGTCTTCT GTATGAAGCT GCCAAGATGT TTGGCCTCCG GAGCAGGAAG
    CTAAAGCTGT TTCTGAATGA GACTCAAACA GAAGAAATTA CAGAAGAAAT CCCTATGAAG
    ACTTTGAATA CGAAGACTGT GTATGTTTCT GTGTTACCAA CTACAGCAGA CTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40687
    Clone ID Related Accession (Same CDS sequence) NM_001101069.2
    Accession Version NM_001101069.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Bos taurus(cattle)
    Product isoleucine--tRNA ligase, cytoplasmic
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151484.1. On Aug 31, 2012 this sequence version replaced NM_001101069.1. ##Evidence-Data-START## Transcript exon combination :: BC151484.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCAGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001094539.1
    CDS71..3859
    Translation

    Target ORF information:

    RefSeq Version NM_001101069.2
    Organism Bos taurus(cattle)
    Definition Bos taurus isoleucyl-tRNA synthetase 1 (IARS1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001101069.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCAGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22284
    Clone ID Related Accession (Same CDS sequence) XM_005210426.4 , XM_005210426.3
    Accession Version XM_005210426.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210426.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210483.1
    CDS152..3940
    Translation

    Target ORF information:

    RefSeq Version XM_005210426.4
    Organism Bos taurus(cattle)
    Definition Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210426.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22284
    Clone ID Related Accession (Same CDS sequence) XM_005210426.4 , XM_005210426.3
    Accession Version XM_005210426.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3789bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product isoleucine--tRNA ligase, cytoplasmic isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104132.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210426.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210483.1
    CDS127..3915
    Translation

    Target ORF information:

    RefSeq Version XM_005210426.3
    Organism Bos taurus(cattle)
    Definition Bos taurus isoleucyl-tRNA synthetase (IARS), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210426.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGTTCAAC AAGTTCCAGA AAACATCAGT TTTCCTGCTG AAGAAGAGAA AATCTTGCAG 
    TTTTGGTCTG ACTTTAATTG TTTCCAGGAA TGCTTAAAGC AATCAAAACA TAGGCCAAAA
    TTTACCTTCT ATGATGGGCC TCCTTTTGCA ACTGGACTGC CTCACTATGG ACATATACTT
    GCAGGGACAA TTAAAGATAT AGTTACAAGA TATGCTCACC AGAGTGGGTT TCACGTTGAC
    AGAAGATTTG GCTGGGATTG TCATGGTTTA CCTGTGGAAT ATGAAATTGA TAAGACCCTG
    GGAATCAGAG GACCAGAGGA TGTAGCCAAA TTGGGGATTG CAGAGTATAA CAGTCAGTGC
    CGAGCAATTG TGATGCGATA CTCTACTGAA TGGAAGTCTA CTATTACCAG ACTTGGCCGA
    TGGATTGACT TTGACAATGA CTACAAAACT CTGTATCCAC AATTCATGGA ATCTGTTTGG
    TGGATCTTCA AACAACTCTA TGATAAAGGC CTTGTTTATA GAGGTGTGAA AGTCATGCCC
    TTCTCCACTG CATGCAATAC TCCACTCTCC AACTTTGAAT CTCACCAGAA TTACAAGGAT
    GTTCAAGATC CTTCAATATT TGTAACTTTC CCTTTGGAGG AAGATGAAAA TATTTCTTTG
    GTTGCCTGGA CAACTACTCC CTGGACTCTT CCTAGTAACC TTGCCCTGTG TGTTAATCCC
    GACTTGCAAT ATGTGAAGAT TAAAGATTCT GTCAGAGGAA AATTACTCAT TTTAATGGAA
    GCCAGATTAT CAGCCCTCTA TAAATCGGAG AGTGACTATG AGATCCTTGA AAGATTTCCT
    GGTGCCTCTC TCAGAGGCAA GAAATACCGG CCCCTGTTTG ACTATTTTGT AAAGTGTAAA
    GAGAGTGGTG CTTTTACTGT GCTTGTGGAC AACTATGTGA AAGAGGAAGA AGGCACTGGG
    ATTGTACACC AAGCTCCTTA CTTTGGTGCT GACGATTATC GGGTCTGTAT GGATTTCAAC
    ATCATTCAGA AAGACTCTCT CCCTATTTGC CCTGTGGATG CCTCAGGCTG TTTTACAGCT
    GAAGTGACAG ATTTTGCAGG ACAGTATGTG AAGGATGCTG ACAAAAATAT CATCAAGACT
    CTAAAGGAAC AAGGTCGACT TCTTGTTGCC AGCACCTTCA CCCATAGCTA CCCTTTTTGT
    TGGAGGTCAG ACACTCCACT CATTTACAAA GCAGTGCCTA GCTGGTTCGT TAGGGTGGAG
    CACATGGTGG ATCAGCTGCT GAGGAACAAT GACCTGTGTT ACTGGGTCCC AGAGTTTGTG
    CGAGAAAAGC GATTTGGAAA TTGGCTGAAA GATGCACGTG ACTGGGCAAT TTCCAGAAAC
    AGATACTGGG GCACTCCTAT CCCACTGTGG GTCAGCAGTG ACTTCGAGGA GGTAGTATGT
    ATTGGGTCAA TAGCAGAACT GGAAGAACTA TCAGGAACAA AGATCTCAGA TCTCCACAGA
    GAGAGCATTG ACCATCTGAC CATTCCTTCA CGCTGTGGAA AGGGATTGTT GCATCGTGTC
    TCTGAAGTGT TTGACTGTTG GTTCGAGAGT GGCAGCATGC CATATGCTCA AATTCATTAT
    CCATTTGAAA ACAAGAGGGA GTTTGAGGAT GCATTTCCTG CAGACTTCAT TGCTGAAGGC
    ATTGACCAAA CCAGAGGATG GTTTTACACC TTGCTGGTGC TAGCCACATC TCTCTTTGGA
    CGGCCACCAT TCAAAAATGT GATTGTGAAT GGACTTGTCC TGGCAAGTGA TGGTCAAAAA
    ATGAGCAAAC GCAAAAAGAA TTATCCAGAC CCACTTTCAG TAATTGATAA ATATGGTGCT
    GATGCCCTCA GATTATATCT GATTAACTCC CCTGTGGTGA GAGCAGAAAA CCTTCGCTTT
    AAGGAAGAGG GTGTCCGAGA TGTCCTGAAG GATGTACTGC TCCCTTGGTA CAATGCCTAT
    CGCTTCTTCA TCCAGAACGT CCTAAGGCTA CAGAAGGAAG AAGAAGTGGA ATTCCTTTAC
    AACGAGAACA TGGTTAAAGA AAGTACCAAC ATCACAGACC GGTGGGTCCT GTCCTTTATG
    CAGTCGCTCG TTGGGTTCTT TGAGACCGAA ATGGCAGCTT ACAGGCTCTA TACTGTGGTT
    CCTCGCCTGG TCAAGTTTGT GGATATTTTG ACCAATTGGT ATGTTCGGAT GAACCGTAGA
    AGATTAAAGG GTGAAAATGG AGTAGAGGAT TGTGTTAAGG CCCTGGAGAC CTTGTTTAGT
    GTTCTGCTGT CTTTGTGCAG ACTTATGGCC CCCTATACCC CTTTTCTTAC TGAATTGATG
    TATCAGAATC TGAAGACACT GATTGACCCT GTTTCTGTCC AGGACAAGGA CACATTCAGT
    ATTCACTACC TCATGCTGCC CCATGTTCGT GAGGAATTGA TCGACAAGAA AACTGAGAGT
    GCAGTATCTA GAATGCAGTC TGTGATTGAA CTTGGCCGAG TGATTCGAGA CCGCAAAACT
    ATTCCAATAA AGTATCCTTT GAAAGAAATT GTTGTTATCC ATCAAGATCA GGAAGCTCTT
    AATGAAGTTA AGTCTTTGGA GAAATATATC ATTGAGGAAT TGAATGTTCG AAAAGTTACA
    TTATCTACAG ATAAAAACAA GTATGGCGTT CGCCTAAGGG CAGAGCCAGA TCACATGGTC
    CTGGGGAAGC GTCTGAAAGG AGCTTTCAAG ATGGTAATGA CATCCATCAA ACAGCTGAGC
    AATGAGGAGC TGGAGCGGTT CCAGGAAAAT GGGACCATTG TTGTGGAAGG CCATGAATTG
    CATGAAGAAG ATATCCGTCT CATATACACC TTTGATCAGG CAACAGGTGG AACAACACAG
    TTTGAAGCAC ACTCGGATGC CCAGGTTTTG GTTCTCTTAG ATGTCACCCC TGACCAGTCA
    ATGGTGGATG AAGGAATGGC TCGGGAAGTC ATCAATCGTA TCCAGAAACT TCGCAAAAAG
    TGTAATCTGG TTCCAACTGA TGAAATAACA GTTTATTATA AAGCAACTTC TGAGGGAAAA
    TATCTGAATA ATGTTATCGA AAGCCACACA GACTTCATAT TTGCTACCAT AAAGTCTCCT
    TTGAAACCAT ATCCAGTTCC TATATCGGAT GAAATACTTA TTCAAGAAAA AATGCAGTTA
    AAAGGGTCTG ACCTGGAAAT TACACTCACC AAAGGATCTT CCACGCATGG CCCTGCTTGT
    GCATATGTCA ATCTTAACAT TTGTACAAAT GGCAGTGAGC AAGGTGGAGT ATTACTTCTG
    GAAAATCCAA AAGGAGATAA TAGGTTGGAC CTTGTAAAAC TGAAGAATGT TGTTACTAGC
    ATTTTTGGTG TAAAAAATAC AGAGCTGGCT GTCCTCCTTG GTGAAACAGA AATAAAAAAC
    CAAACCAACT TACTGAATCT TAGTGGGAAA ACACTGTGTG TGACTGCAGG ATCAGCGCTG
    TCTGTGGTGG ACAGTCCTAG TACTCTTCTC TGTCAGTACA TCAACCTACA GCTCTTGAAT
    GCAGATCCAC AAGAGTGTTT AATGGGAACA GTAGGCACTC TTCTGCTTGA AAATCCACTT
    GGACAGAATG GGCTTACCCA CCAAGGTCTT CTGTATGAAG CTGCCAAGAT GTTTGGCCTC
    CGGAGCAGGA AGCTAAAGCT GTTTCTGAAT GAGACTCAAA CAGAAGAAAT TACAGAAGAA
    ATCCCTATGA AGACTTTGAA TACGAAGACT GTGTATGTTT CTGTGTTACC AACTACAGCA
    GACTTCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.