TLE4 cDNA ORF clone, Bos taurus(cattle)

The following TLE4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TLE4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa40324 NM_001081583.1
Latest version!
Bos taurus TLE family member 4, transcriptional corepressor (TLE4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa22033 XM_005210076.4
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47285 XM_010807925.3
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47286 XM_010807926.2
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47287 XM_010807927.3
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47288 XM_010807928.3
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47289 XM_010807929.2
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa236993 XM_024995558.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236994 XM_024995559.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236995 XM_024995560.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236996 XM_024995561.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236997 XM_024995562.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236998 XM_024995563.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa236999 XM_024995564.1
Latest version!
Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OBa47285 XM_010807925.2 Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47287 XM_010807927.2 Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa22033 XM_005210076.3 Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa47288 XM_010807928.2 Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa40324
    Clone ID Related Accession (Same CDS sequence) NM_001081583.1
    Accession Version NM_001081583.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-17
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133368.1. On or before Feb 20, 2007 this sequence version replaced XM_873914.2, XM_001249430.1. ##Evidence-Data-START## Transcript exon combination :: BC133368.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145419 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCACCA GCAACAAGTG GTGCAGGCTG TGGAACGGGC CAAGCAGGTG
    ACCATGGCAG AACTGAACGC CATCATTGGG CAGCAGCAAC TCCAGGCCCA GCATCTATCA
    CATGGACATG GTCTGCCAGT GCCTCTGACC CCACACCCTT CGGGGCTTCA GCCTCCTGCC
    ATTCCGCCCA TTGGTAGCAG TGCCGGCCTT CTGGCCCTCT CCAGTGCCCT GGGAGGCCAG
    TCCCACCTTC CAATTAAAGA TGAGAAGAAG CATCATGACA GTGATCACCA AAGAGACAGA
    GACTCCATCA AGAGCTCTTC GGTATCCCCA TCAGCCAGTT TCCGAGGTGC TGAGAAGCAC
    AGAAACTCCA CAGACTATTC CTCTGATAGC AAAAAGCAGA AAACTGAAGA AAAGGAGATT
    GCAGCTCGTT ATGACAGCGA TGCTGAGAAG AGTGATGACA ACTTGGTGGT TGACGTTTCC
    AATGAGGATC CATCATCACC TCGAGGGAGC CCAGCACATT CCCCGAGAGA GAACGGCCTG
    GACAAGACAC GCCTGCTCAA GAAAGATGCC CCAATCAGTC CAGCTTCGAT TGCATCCTCC
    AGCAGTACTC CTTCCTCCAA ATCCAAAGAA CTTAGCCTTA ACGAAAAATC TACTACTCCT
    GTGTCGAAGT CCAATACCCC TACTCCACGA ACTGATGCAC CCACCCCAGG CAGTAATTCC
    ACTCCCGGAT TGAGGCCGGT TCCCGGAAAA CCGCCAGGAG TCGACCCGTT GGCTTCAAGC
    CTAAGGACTC CCATGGCAGT ACCTTGTCCA TACCCAACCC CGTTTGGGAT TGTGCCACAC
    GCGGGGATGA ATGGGGAGCT GACCAGCCCC GGAGCTGCCT ACGCAGGGCT ACATAACATC
    TCCCCACAGA TGAGCGCGGC TGCTGCTGCT GCCGCCGCGG CTGCTGCCTA CGGGAGATCA
    CCAGTGGTTG GATTTGATCC ACACCATCAC ATGCGTGTGC CAGCGATACC TCCAAACCTG
    ACAGGCATTC CAGGAGGAAA GCCGGCATAC TCCTTCCACG TTACCGCAGA TGGTCAGATG
    CAGCCTGTCC CCTTCCCCCC GGACGCCCTT ATCGGCCCCG GCATTCCTCG ACACGCTCGC
    CAGATCAACA CCCTAAACCA TGGGGAGGTG GTGTGTGCGG TGACCATCAG CAACCCCACG
    CGACACGTGT ACACGGGTGG GAAGGGCTGC GTCAAGGTCT GGGACATCAG CCACCCTGGC
    AACAAGAGCC CCGTCTCTCA GCTCGACTGT CTGAACAGGG ATAACTACAT CCGTTCCTGC
    CGATTGCTCC CTGATGGTCG TACCCTAATT GTGGGAGGGG AAGCCAGTAC GCTGTCCATC
    TGGGACCTGG CAGCTCCCAC CCCGCGCATC AAGGCAGAGC TGACGTCCTC AGCCCCCGCC
    TGCTACGCCC TGGCCATCAG CCCGGACTCC AAGGTCTGCT TCTCGTGCTG CAGTGACGGC
    AACATTGCTG TGTGGGACCT GCACAACCAG ACGCTGGTGA GGCAATTCCA GGGCCACACG
    GACGGGGCCA GCTGTATTGA CATCTCTAAT GATGGCACCA AGCTCTGGAC GGGTGGCTTG
    GACAACACGG TCCGGTCCTG GGACCTTCGT GAGGGGCGGC AGCTGCAGCA GCACGACTTC
    ACCTCCCAGA TCTTTTCTCT GGGCTACTGC CCGACCGGAG AGTGGCTGGC TGTGGGGATG
    GAAAACAGCA ACGTGGAGGT GTTGCATGTC ACCAAGCCCG ACAAGTACCA GCTCCACCTG
    CACGAGAGCT GCGTGCTGTC GCTCAAGTTT GCCCACTGTG GCAAATGGTT TGTCAGCACG
    GGAAAGGACA ACCTTCTGAA TGCCTGGAGA ACACCTTATG GGGCCAGTAT ATTCCAGTCC
    AAAGAATCCT CATCGGTGCT CAGCTGTGAC ATTTCTGTGG ATGACAAATA CATTGTCACT
    GGCTCTGGGG ATAAGAAGGC CACAGTTTAT GAAGTTATTT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075052.1
    CDS249..2573
    Translation

    Target ORF information:

    RefSeq Version NM_001081583.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TLE family member 4, transcriptional corepressor (TLE4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001081583.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCACCA GCAACAAGTG GTGCAGGCTG TGGAACGGGC CAAGCAGGTG
    ACCATGGCAG AACTGAACGC CATCATTGGG CAGCAGCAAC TCCAGGCCCA GCATCTATCA
    CATGGACATG GTCTGCCAGT GCCTCTGACC CCACACCCTT CGGGGCTTCA GCCTCCTGCC
    ATTCCGCCCA TTGGTAGCAG TGCCGGCCTT CTGGCCCTCT CCAGTGCCCT GGGAGGCCAG
    TCCCACCTTC CAATTAAAGA TGAGAAGAAG CATCATGACA GTGATCACCA AAGAGACAGA
    GACTCCATCA AGAGCTCTTC GGTATCCCCA TCAGCCAGTT TCCGAGGTGC TGAGAAGCAC
    AGAAACTCCA CAGACTATTC CTCTGATAGC AAAAAGCAGA AAACTGAAGA AAAGGAGATT
    GCAGCTCGTT ATGACAGCGA TGCTGAGAAG AGTGATGACA ACTTGGTGGT TGACGTTTCC
    AATGAGGATC CATCATCACC TCGAGGGAGC CCAGCACATT CCCCGAGAGA GAACGGCCTG
    GACAAGACAC GCCTGCTCAA GAAAGATGCC CCAATCAGTC CAGCTTCGAT TGCATCCTCC
    AGCAGTACTC CTTCCTCCAA ATCCAAAGAA CTTAGCCTTA ACGAAAAATC TACTACTCCT
    GTGTCGAAGT CCAATACCCC TACTCCACGA ACTGATGCAC CCACCCCAGG CAGTAATTCC
    ACTCCCGGAT TGAGGCCGGT TCCCGGAAAA CCGCCAGGAG TCGACCCGTT GGCTTCAAGC
    CTAAGGACTC CCATGGCAGT ACCTTGTCCA TACCCAACCC CGTTTGGGAT TGTGCCACAC
    GCGGGGATGA ATGGGGAGCT GACCAGCCCC GGAGCTGCCT ACGCAGGGCT ACATAACATC
    TCCCCACAGA TGAGCGCGGC TGCTGCTGCT GCCGCCGCGG CTGCTGCCTA CGGGAGATCA
    CCAGTGGTTG GATTTGATCC ACACCATCAC ATGCGTGTGC CAGCGATACC TCCAAACCTG
    ACAGGCATTC CAGGAGGAAA GCCGGCATAC TCCTTCCACG TTACCGCAGA TGGTCAGATG
    CAGCCTGTCC CCTTCCCCCC GGACGCCCTT ATCGGCCCCG GCATTCCTCG ACACGCTCGC
    CAGATCAACA CCCTAAACCA TGGGGAGGTG GTGTGTGCGG TGACCATCAG CAACCCCACG
    CGACACGTGT ACACGGGTGG GAAGGGCTGC GTCAAGGTCT GGGACATCAG CCACCCTGGC
    AACAAGAGCC CCGTCTCTCA GCTCGACTGT CTGAACAGGG ATAACTACAT CCGTTCCTGC
    CGATTGCTCC CTGATGGTCG TACCCTAATT GTGGGAGGGG AAGCCAGTAC GCTGTCCATC
    TGGGACCTGG CAGCTCCCAC CCCGCGCATC AAGGCAGAGC TGACGTCCTC AGCCCCCGCC
    TGCTACGCCC TGGCCATCAG CCCGGACTCC AAGGTCTGCT TCTCGTGCTG CAGTGACGGC
    AACATTGCTG TGTGGGACCT GCACAACCAG ACGCTGGTGA GGCAATTCCA GGGCCACACG
    GACGGGGCCA GCTGTATTGA CATCTCTAAT GATGGCACCA AGCTCTGGAC GGGTGGCTTG
    GACAACACGG TCCGGTCCTG GGACCTTCGT GAGGGGCGGC AGCTGCAGCA GCACGACTTC
    ACCTCCCAGA TCTTTTCTCT GGGCTACTGC CCGACCGGAG AGTGGCTGGC TGTGGGGATG
    GAAAACAGCA ACGTGGAGGT GTTGCATGTC ACCAAGCCCG ACAAGTACCA GCTCCACCTG
    CACGAGAGCT GCGTGCTGTC GCTCAAGTTT GCCCACTGTG GCAAATGGTT TGTCAGCACG
    GGAAAGGACA ACCTTCTGAA TGCCTGGAGA ACACCTTATG GGGCCAGTAT ATTCCAGTCC
    AAAGAATCCT CATCGGTGCT CAGCTGTGAC ATTTCTGTGG ATGACAAATA CATTGTCACT
    GGCTCTGGGG ATAAGAAGGC CACAGTTTAT GAAGTTATTT ATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22033
    Clone ID Related Accession (Same CDS sequence) XM_005210076.4 , XM_005210076.3
    Accession Version XM_005210076.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005210076.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210133.1
    CDS113..2431
    Translation

    Target ORF information:

    RefSeq Version XM_005210076.4
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210076.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa22033
    Clone ID Related Accession (Same CDS sequence) XM_005210076.4 , XM_005210076.3
    Accession Version XM_005210076.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2319bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210076.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005210133.1
    CDS92..2410
    Translation

    Target ORF information:

    RefSeq Version XM_005210076.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005210076.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47285
    Clone ID Related Accession (Same CDS sequence) XM_010807925.3 , XM_010807925.2
    Accession Version XM_010807925.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807925.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806227.1
    CDS112..2427
    Translation

    Target ORF information:

    RefSeq Version XM_010807925.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807925.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47285
    Clone ID Related Accession (Same CDS sequence) XM_010807925.3 , XM_010807925.2
    Accession Version XM_010807925.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2316bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806227.1
    CDS92..2407
    Translation

    Target ORF information:

    RefSeq Version XM_010807925.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807925.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC ACCAGCAACA AGTGGTGCAG GCTGTGGAAC GGGCCAAGCA GGTGACCATG
    GCAGAACTGA ACGCCATCAT TGGGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47286
    Clone ID Related Accession (Same CDS sequence) XM_010807926.2
    Accession Version XM_010807926.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2256bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807926.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCACC AGCAACAAGT GGTGCAGGCT GTGGAACGGG CCAAGCAGGT GACCATGGCA
    GAACTGAACG CCATCATTGG GCAGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806228.1
    CDS92..2347
    Translation

    Target ORF information:

    RefSeq Version XM_010807926.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807926.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCACC AGCAACAAGT GGTGCAGGCT GTGGAACGGG CCAAGCAGGT GACCATGGCA
    GAACTGAACG CCATCATTGG GCAGCAGCAA CTCCAGGCCC AGCATCTATC ACATGGACAT
    GGTCTGCCAG TGCCTCTGAC CCCACACCCT TCGGGGCTTC AGCCTCCTGC CATTCCGCCC
    ATTGGTAGCA GTGCCGGCCT TCTGGCCCTC TCCAGTGCCC TGGGAGGCCA GTCCCACCTT
    CCAATTAAAG ATGAGAAGAA GCATCATGAC AGTGATCACC AAAGAGACAG AGACTCCATC
    AAGAGCTCTT CGGTATCCCC ATCAGCCAGT TTCCGAGGTG CTGAGAAGCA CAGAAACTCC
    ACAGACTATT CCTCTGATAG CAAAAAGCAG AAAACTGAAG AAAAGGAGAT TGCAGCTCGT
    TATGACAGCG ATGCTGAGAA GAGTGATGAC AACTTGGTGG TTGACGTTTC CAATGAGGAT
    CCATCATCAC CTCGAGGGAG CCCAGCACAT TCCCCGAGAG AGAACGGCCT GGACAAGACA
    CGCCTGCTCA AGAAAGATGC CCCAATCAGT CCAGCTTCGA TTGCATCCTC CAGCAGTACT
    CCTTCCTCCA AATCCAAAGA ACTTAGCCTT AACGAAAAAT CTACTACTCC TGTGTCGAAG
    TCCAATACCC CTACTCCACG AACTGATGCA CCCACCCCAG GCAGTAATTC CACTCCCGGA
    TTGAGGCCGG TTCCCGGAAA ACCGCCAGGA GTCGACCCGT TGGCTTCAAG CCTAAGGACT
    CCCATGGCAG TACCTTGTCC ATACCCAACC CCGTTTGGGA TTGTGCCACA CGCGGGGATG
    AATGGGGAGC TGACCAGCCC CGGAGCTGCC TACGCAGGGC TACATAACAT CTCCCCACAG
    ATGAGCGCGG CTGCTGCTGC TGCCGCCGCG GCTGCTGCCT ACGGGAGATC ACCAGTGGTT
    GGATTTGATC CACACCATCA CATGCGTGTG CCAGCGATAC CTCCAAACCT GACAGGCATT
    CCAGGAGGAA AGCCGGCATA CTCCTTCCAC GTTACCGCAG ATGGTCAGAT GCAGCCTGTC
    CCCTTCCCCC CGGACGCCCT TATCGGCCCC GGCATTCCTC GACACGCTCG CCAGATCAAC
    ACCCTAAACC ATGGGGAGGT GGTGTGTGCG GTGACCATCA GCAACCCCAC GCGACACGTG
    TACACGGGTG GGAAGGGCTG CGTCAAGGTC TGGGACATCA GCCACCCTGG CAACAAGAGC
    CCCGTCTCTC AGCTCGACTG TCTGAACAGG GATAACTACA TCCGTTCCTG CCGATTGCTC
    CCTGATGGTC GTACCCTAAT TGTGGGAGGG GAAGCCAGTA CGCTGTCCAT CTGGGACCTG
    GCAGCTCCCA CCCCGCGCAT CAAGGCAGAG CTGACGTCCT CAGCCCCCGC CTGCTACGCC
    CTGGCCATCA GCCCGGACTC CAAGGTCTGC TTCTCGTGCT GCAGTGACGG CAACATTGCT
    GTGTGGGACC TGCACAACCA GACGCTGGTG AGGCAATTCC AGGGCCACAC GGACGGGGCC
    AGCTGTATTG ACATCTCTAA TGATGGCACC AAGCTCTGGA CGGGTGGCTT GGACAACACG
    GTCCGGTCCT GGGACCTTCG TGAGGGGCGG CAGCTGCAGC AGCACGACTT CACCTCCCAG
    ATCTTTTCTC TGGGCTACTG CCCGACCGGA GAGTGGCTGG CTGTGGGGAT GGAAAACAGC
    AACGTGGAGG TGTTGCATGT CACCAAGCCC GACAAGTACC AGCTCCACCT GCACGAGAGC
    TGCGTGCTGT CGCTCAAGTT TGCCCACTGT GGCAAATGGT TTGTCAGCAC GGGAAAGGAC
    AACCTTCTGA ATGCCTGGAG AACACCTTAT GGGGCCAGTA TATTCCAGTC CAAAGAATCC
    TCATCGGTGC TCAGCTGTGA CATTTCTGTG GATGACAAAT ACATTGTCAC TGGCTCTGGG
    GATAAGAAGG CCACAGTTTA TGAAGTTATT TATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47287
    Clone ID Related Accession (Same CDS sequence) XM_010807927.3 , XM_010807927.2
    Accession Version XM_010807927.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAGCAACT CCAGGCCCAG CATCTATCAC ATGGACATGG TCTGCCAGTG
    CCTCTGACCC CACACCCTTC GGGGCTTCAG CCTCCTGCCA TTCCGCCCAT TGGTAGCAGT
    GCCGGCCTTC TGGCCCTCTC CAGTGCCCTG GGAGGCCAGT CCCACCTTCC AATTAAAGAT
    GAGAAGAAGC ATCATGACAG TGATCACCAA AGAGACAGAG ACTCCATCAA GAGCTCTTCG
    GTATCCCCAT CAGCCAGTTT CCGAGGTGCT GAGAAGCACA GAAACTCCAC AGACTATTCC
    TCTGATAGCA AAAAGCAGAA AACTGAAGAA AAGGAGATTG CAGCTCGTTA TGACAGCGAT
    GCTGAGAAGA GTGATGACAA CTTGGTGGTT GACGTTTCCA ATGAGGATCC ATCATCACCT
    CGAGGGAGCC CAGCACATTC CCCGAGAGAG AACGGCCTGG ACAAGACACG CCTGCTCAAG
    AAAGATGCCC CAATCAGTCC AGCTTCGATT GCATCCTCCA GCAGTACTCC TTCCTCCAAA
    TCCAAAGAAC TTAGCCTTAA CGAAAAATCT ACTACTCCTG TGTCGAAGTC CAATACCCCT
    ACTCCACGAA CTGATGCACC CACCCCAGGC AGTAATTCCA CTCCCGGATT GAGGCCGGTT
    CCCGGAAAAC CGCCAGGAGT CGACCCGTTG GCTTCAAGCC TAAGGACTCC CATGGCAGTA
    CCTTGTCCAT ACCCAACCCC GTTTGGGATT GTGCCACACG CGGGGATGAA TGGGGAGCTG
    ACCAGCCCCG GAGCTGCCTA CGCAGGGCTA CATAACATCT CCCCACAGAT GAGCGCGGCT
    GCTGCTGCTG CCGCCGCGGC TGCTGCCTAC GGGAGATCAC CAGTGGTTGG ATTTGATCCA
    CACCATCACA TGCGTGTGCC AGCGATACCT CCAAACCTGA CAGGCATTCC AGGAGGAAAG
    CCGGCATACT CCTTCCACGT TACCGCAGAT GGTCAGATGC AGCCTGTCCC CTTCCCCCCG
    GACGCCCTTA TCGGCCCCGG CATTCCTCGA CACGCTCGCC AGATCAACAC CCTAAACCAT
    GGGGAGGTGG TGTGTGCGGT GACCATCAGC AACCCCACGC GACACGTGTA CACGGGTGGG
    AAGGGCTGCG TCAAGGTCTG GGACATCAGC CACCCTGGCA ACAAGAGCCC CGTCTCTCAG
    CTCGACTGTC TGAACAGGGA TAACTACATC CGTTCCTGCC GATTGCTCCC TGATGGTCGT
    ACCCTAATTG TGGGAGGGGA AGCCAGTACG CTGTCCATCT GGGACCTGGC AGCTCCCACC
    CCGCGCATCA AGGCAGAGCT GACGTCCTCA GCCCCCGCCT GCTACGCCCT GGCCATCAGC
    CCGGACTCCA AGGTCTGCTT CTCGTGCTGC AGTGACGGCA ACATTGCTGT GTGGGACCTG
    CACAACCAGA CGCTGGTGAG GCAATTCCAG GGCCACACGG ACGGGGCCAG CTGTATTGAC
    ATCTCTAATG ATGGCACCAA GCTCTGGACG GGTGGCTTGG ACAACACGGT CCGGTCCTGG
    GACCTTCGTG AGGGGCGGCA GCTGCAGCAG CACGACTTCA CCTCCCAGAT CTTTTCTCTG
    GGCTACTGCC CGACCGGAGA GTGGCTGGCT GTGGGGATGG AAAACAGCAA CGTGGAGGTG
    TTGCATGTCA CCAAGCCCGA CAAGTACCAG CTCCACCTGC ACGAGAGCTG CGTGCTGTCG
    CTCAAGTTTG CCCACTGTGG CAAATGGTTT GTCAGCACGG GAAAGGACAA CCTTCTGAAT
    GCCTGGAGAA CACCTTATGG GGCCAGTATA TTCCAGTCCA AAGAATCCTC ATCGGTGCTC
    AGCTGTGACA TTTCTGTGGA TGACAAATAC ATTGTCACTG GCTCTGGGGA TAAGAAGGCC
    ACAGTTTATG AAGTTATTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806229.1
    CDS113..2356
    Translation

    Target ORF information:

    RefSeq Version XM_010807927.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807927.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAGCAACT CCAGGCCCAG CATCTATCAC ATGGACATGG TCTGCCAGTG
    CCTCTGACCC CACACCCTTC GGGGCTTCAG CCTCCTGCCA TTCCGCCCAT TGGTAGCAGT
    GCCGGCCTTC TGGCCCTCTC CAGTGCCCTG GGAGGCCAGT CCCACCTTCC AATTAAAGAT
    GAGAAGAAGC ATCATGACAG TGATCACCAA AGAGACAGAG ACTCCATCAA GAGCTCTTCG
    GTATCCCCAT CAGCCAGTTT CCGAGGTGCT GAGAAGCACA GAAACTCCAC AGACTATTCC
    TCTGATAGCA AAAAGCAGAA AACTGAAGAA AAGGAGATTG CAGCTCGTTA TGACAGCGAT
    GCTGAGAAGA GTGATGACAA CTTGGTGGTT GACGTTTCCA ATGAGGATCC ATCATCACCT
    CGAGGGAGCC CAGCACATTC CCCGAGAGAG AACGGCCTGG ACAAGACACG CCTGCTCAAG
    AAAGATGCCC CAATCAGTCC AGCTTCGATT GCATCCTCCA GCAGTACTCC TTCCTCCAAA
    TCCAAAGAAC TTAGCCTTAA CGAAAAATCT ACTACTCCTG TGTCGAAGTC CAATACCCCT
    ACTCCACGAA CTGATGCACC CACCCCAGGC AGTAATTCCA CTCCCGGATT GAGGCCGGTT
    CCCGGAAAAC CGCCAGGAGT CGACCCGTTG GCTTCAAGCC TAAGGACTCC CATGGCAGTA
    CCTTGTCCAT ACCCAACCCC GTTTGGGATT GTGCCACACG CGGGGATGAA TGGGGAGCTG
    ACCAGCCCCG GAGCTGCCTA CGCAGGGCTA CATAACATCT CCCCACAGAT GAGCGCGGCT
    GCTGCTGCTG CCGCCGCGGC TGCTGCCTAC GGGAGATCAC CAGTGGTTGG ATTTGATCCA
    CACCATCACA TGCGTGTGCC AGCGATACCT CCAAACCTGA CAGGCATTCC AGGAGGAAAG
    CCGGCATACT CCTTCCACGT TACCGCAGAT GGTCAGATGC AGCCTGTCCC CTTCCCCCCG
    GACGCCCTTA TCGGCCCCGG CATTCCTCGA CACGCTCGCC AGATCAACAC CCTAAACCAT
    GGGGAGGTGG TGTGTGCGGT GACCATCAGC AACCCCACGC GACACGTGTA CACGGGTGGG
    AAGGGCTGCG TCAAGGTCTG GGACATCAGC CACCCTGGCA ACAAGAGCCC CGTCTCTCAG
    CTCGACTGTC TGAACAGGGA TAACTACATC CGTTCCTGCC GATTGCTCCC TGATGGTCGT
    ACCCTAATTG TGGGAGGGGA AGCCAGTACG CTGTCCATCT GGGACCTGGC AGCTCCCACC
    CCGCGCATCA AGGCAGAGCT GACGTCCTCA GCCCCCGCCT GCTACGCCCT GGCCATCAGC
    CCGGACTCCA AGGTCTGCTT CTCGTGCTGC AGTGACGGCA ACATTGCTGT GTGGGACCTG
    CACAACCAGA CGCTGGTGAG GCAATTCCAG GGCCACACGG ACGGGGCCAG CTGTATTGAC
    ATCTCTAATG ATGGCACCAA GCTCTGGACG GGTGGCTTGG ACAACACGGT CCGGTCCTGG
    GACCTTCGTG AGGGGCGGCA GCTGCAGCAG CACGACTTCA CCTCCCAGAT CTTTTCTCTG
    GGCTACTGCC CGACCGGAGA GTGGCTGGCT GTGGGGATGG AAAACAGCAA CGTGGAGGTG
    TTGCATGTCA CCAAGCCCGA CAAGTACCAG CTCCACCTGC ACGAGAGCTG CGTGCTGTCG
    CTCAAGTTTG CCCACTGTGG CAAATGGTTT GTCAGCACGG GAAAGGACAA CCTTCTGAAT
    GCCTGGAGAA CACCTTATGG GGCCAGTATA TTCCAGTCCA AAGAATCCTC ATCGGTGCTC
    AGCTGTGACA TTTCTGTGGA TGACAAATAC ATTGTCACTG GCTCTGGGGA TAAGAAGGCC
    ACAGTTTATG AAGTTATTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47287
    Clone ID Related Accession (Same CDS sequence) XM_010807927.3 , XM_010807927.2
    Accession Version XM_010807927.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807927.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAGCAACT CCAGGCCCAG CATCTATCAC ATGGACATGG TCTGCCAGTG
    CCTCTGACCC CACACCCTTC GGGGCTTCAG CCTCCTGCCA TTCCGCCCAT TGGTAGCAGT
    GCCGGCCTTC TGGCCCTCTC CAGTGCCCTG GGAGGCCAGT CCCACCTTCC AATTAAAGAT
    GAGAAGAAGC ATCATGACAG TGATCACCAA AGAGACAGAG ACTCCATCAA GAGCTCTTCG
    GTATCCCCAT CAGCCAGTTT CCGAGGTGCT GAGAAGCACA GAAACTCCAC AGACTATTCC
    TCTGATAGCA AAAAGCAGAA AACTGAAGAA AAGGAGATTG CAGCTCGTTA TGACAGCGAT
    GCTGAGAAGA GTGATGACAA CTTGGTGGTT GACGTTTCCA ATGAGGATCC ATCATCACCT
    CGAGGGAGCC CAGCACATTC CCCGAGAGAG AACGGCCTGG ACAAGACACG CCTGCTCAAG
    AAAGATGCCC CAATCAGTCC AGCTTCGATT GCATCCTCCA GCAGTACTCC TTCCTCCAAA
    TCCAAAGAAC TTAGCCTTAA CGAAAAATCT ACTACTCCTG TGTCGAAGTC CAATACCCCT
    ACTCCACGAA CTGATGCACC CACCCCAGGC AGTAATTCCA CTCCCGGATT GAGGCCGGTT
    CCCGGAAAAC CGCCAGGAGT CGACCCGTTG GCTTCAAGCC TAAGGACTCC CATGGCAGTA
    CCTTGTCCAT ACCCAACCCC GTTTGGGATT GTGCCACACG CGGGGATGAA TGGGGAGCTG
    ACCAGCCCCG GAGCTGCCTA CGCAGGGCTA CATAACATCT CCCCACAGAT GAGCGCGGCT
    GCTGCTGCTG CCGCCGCGGC TGCTGCCTAC GGGAGATCAC CAGTGGTTGG ATTTGATCCA
    CACCATCACA TGCGTGTGCC AGCGATACCT CCAAACCTGA CAGGCATTCC AGGAGGAAAG
    CCGGCATACT CCTTCCACGT TACCGCAGAT GGTCAGATGC AGCCTGTCCC CTTCCCCCCG
    GACGCCCTTA TCGGCCCCGG CATTCCTCGA CACGCTCGCC AGATCAACAC CCTAAACCAT
    GGGGAGGTGG TGTGTGCGGT GACCATCAGC AACCCCACGC GACACGTGTA CACGGGTGGG
    AAGGGCTGCG TCAAGGTCTG GGACATCAGC CACCCTGGCA ACAAGAGCCC CGTCTCTCAG
    CTCGACTGTC TGAACAGGGA TAACTACATC CGTTCCTGCC GATTGCTCCC TGATGGTCGT
    ACCCTAATTG TGGGAGGGGA AGCCAGTACG CTGTCCATCT GGGACCTGGC AGCTCCCACC
    CCGCGCATCA AGGCAGAGCT GACGTCCTCA GCCCCCGCCT GCTACGCCCT GGCCATCAGC
    CCGGACTCCA AGGTCTGCTT CTCGTGCTGC AGTGACGGCA ACATTGCTGT GTGGGACCTG
    CACAACCAGA CGCTGGTGAG GCAATTCCAG GGCCACACGG ACGGGGCCAG CTGTATTGAC
    ATCTCTAATG ATGGCACCAA GCTCTGGACG GGTGGCTTGG ACAACACGGT CCGGTCCTGG
    GACCTTCGTG AGGGGCGGCA GCTGCAGCAG CACGACTTCA CCTCCCAGAT CTTTTCTCTG
    GGCTACTGCC CGACCGGAGA GTGGCTGGCT GTGGGGATGG AAAACAGCAA CGTGGAGGTG
    TTGCATGTCA CCAAGCCCGA CAAGTACCAG CTCCACCTGC ACGAGAGCTG CGTGCTGTCG
    CTCAAGTTTG CCCACTGTGG CAAATGGTTT GTCAGCACGG GAAAGGACAA CCTTCTGAAT
    GCCTGGAGAA CACCTTATGG GGCCAGTATA TTCCAGTCCA AAGAATCCTC ATCGGTGCTC
    AGCTGTGACA TTTCTGTGGA TGACAAATAC ATTGTCACTG GCTCTGGGGA TAAGAAGGCC
    ACAGTTTATG AAGTTATTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806229.1
    CDS92..2335
    Translation

    Target ORF information:

    RefSeq Version XM_010807927.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807927.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAGCAACT CCAGGCCCAG CATCTATCAC ATGGACATGG TCTGCCAGTG
    CCTCTGACCC CACACCCTTC GGGGCTTCAG CCTCCTGCCA TTCCGCCCAT TGGTAGCAGT
    GCCGGCCTTC TGGCCCTCTC CAGTGCCCTG GGAGGCCAGT CCCACCTTCC AATTAAAGAT
    GAGAAGAAGC ATCATGACAG TGATCACCAA AGAGACAGAG ACTCCATCAA GAGCTCTTCG
    GTATCCCCAT CAGCCAGTTT CCGAGGTGCT GAGAAGCACA GAAACTCCAC AGACTATTCC
    TCTGATAGCA AAAAGCAGAA AACTGAAGAA AAGGAGATTG CAGCTCGTTA TGACAGCGAT
    GCTGAGAAGA GTGATGACAA CTTGGTGGTT GACGTTTCCA ATGAGGATCC ATCATCACCT
    CGAGGGAGCC CAGCACATTC CCCGAGAGAG AACGGCCTGG ACAAGACACG CCTGCTCAAG
    AAAGATGCCC CAATCAGTCC AGCTTCGATT GCATCCTCCA GCAGTACTCC TTCCTCCAAA
    TCCAAAGAAC TTAGCCTTAA CGAAAAATCT ACTACTCCTG TGTCGAAGTC CAATACCCCT
    ACTCCACGAA CTGATGCACC CACCCCAGGC AGTAATTCCA CTCCCGGATT GAGGCCGGTT
    CCCGGAAAAC CGCCAGGAGT CGACCCGTTG GCTTCAAGCC TAAGGACTCC CATGGCAGTA
    CCTTGTCCAT ACCCAACCCC GTTTGGGATT GTGCCACACG CGGGGATGAA TGGGGAGCTG
    ACCAGCCCCG GAGCTGCCTA CGCAGGGCTA CATAACATCT CCCCACAGAT GAGCGCGGCT
    GCTGCTGCTG CCGCCGCGGC TGCTGCCTAC GGGAGATCAC CAGTGGTTGG ATTTGATCCA
    CACCATCACA TGCGTGTGCC AGCGATACCT CCAAACCTGA CAGGCATTCC AGGAGGAAAG
    CCGGCATACT CCTTCCACGT TACCGCAGAT GGTCAGATGC AGCCTGTCCC CTTCCCCCCG
    GACGCCCTTA TCGGCCCCGG CATTCCTCGA CACGCTCGCC AGATCAACAC CCTAAACCAT
    GGGGAGGTGG TGTGTGCGGT GACCATCAGC AACCCCACGC GACACGTGTA CACGGGTGGG
    AAGGGCTGCG TCAAGGTCTG GGACATCAGC CACCCTGGCA ACAAGAGCCC CGTCTCTCAG
    CTCGACTGTC TGAACAGGGA TAACTACATC CGTTCCTGCC GATTGCTCCC TGATGGTCGT
    ACCCTAATTG TGGGAGGGGA AGCCAGTACG CTGTCCATCT GGGACCTGGC AGCTCCCACC
    CCGCGCATCA AGGCAGAGCT GACGTCCTCA GCCCCCGCCT GCTACGCCCT GGCCATCAGC
    CCGGACTCCA AGGTCTGCTT CTCGTGCTGC AGTGACGGCA ACATTGCTGT GTGGGACCTG
    CACAACCAGA CGCTGGTGAG GCAATTCCAG GGCCACACGG ACGGGGCCAG CTGTATTGAC
    ATCTCTAATG ATGGCACCAA GCTCTGGACG GGTGGCTTGG ACAACACGGT CCGGTCCTGG
    GACCTTCGTG AGGGGCGGCA GCTGCAGCAG CACGACTTCA CCTCCCAGAT CTTTTCTCTG
    GGCTACTGCC CGACCGGAGA GTGGCTGGCT GTGGGGATGG AAAACAGCAA CGTGGAGGTG
    TTGCATGTCA CCAAGCCCGA CAAGTACCAG CTCCACCTGC ACGAGAGCTG CGTGCTGTCG
    CTCAAGTTTG CCCACTGTGG CAAATGGTTT GTCAGCACGG GAAAGGACAA CCTTCTGAAT
    GCCTGGAGAA CACCTTATGG GGCCAGTATA TTCCAGTCCA AAGAATCCTC ATCGGTGCTC
    AGCTGTGACA TTTCTGTGGA TGACAAATAC ATTGTCACTG GCTCTGGGGA TAAGAAGGCC
    ACAGTTTATG AAGTTATTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47288
    Clone ID Related Accession (Same CDS sequence) XM_010807928.2 , XM_010807928.3
    Accession Version XM_010807928.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807928.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806230.1
    CDS92..2332
    Translation

    Target ORF information:

    RefSeq Version XM_010807928.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807928.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47288
    Clone ID Related Accession (Same CDS sequence) XM_010807928.2 , XM_010807928.3
    Accession Version XM_010807928.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2241bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807928.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806230.1
    CDS113..2353
    Translation

    Target ORF information:

    RefSeq Version XM_010807928.3
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807928.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGGCAG AGATTGTCAA GAGGCTGAAC GCTATCTGTG CACAAGTCAT TCCTTTCCTG
    TCCCAAGAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47289
    Clone ID Related Accession (Same CDS sequence) XM_010807929.2
    Accession Version XM_010807929.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2181bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807929.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010806231.1
    CDS92..2272
    Translation

    Target ORF information:

    RefSeq Version XM_010807929.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807929.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCAGC AGCAACTCCA GGCCCAGCAT CTATCACATG GACATGGTCT GCCAGTGCCT
    CTGACCCCAC ACCCTTCGGG GCTTCAGCCT CCTGCCATTC CGCCCATTGG TAGCAGTGCC
    GGCCTTCTGG CCCTCTCCAG TGCCCTGGGA GGCCAGTCCC ACCTTCCAAT TAAAGATGAG
    AAGAAGCATC ATGACAGTGA TCACCAAAGA GACAGAGACT CCATCAAGAG CTCTTCGGTA
    TCCCCATCAG CCAGTTTCCG AGGTGCTGAG AAGCACAGAA ACTCCACAGA CTATTCCTCT
    GATAGCAAAA AGCAGAAAAC TGAAGAAAAG GAGATTGCAG CTCGTTATGA CAGCGATGCT
    GAGAAGAGTG ATGACAACTT GGTGGTTGAC GTTTCCAATG AGGATCCATC ATCACCTCGA
    GGGAGCCCAG CACATTCCCC GAGAGAGAAC GGCCTGGACA AGACACGCCT GCTCAAGAAA
    GATGCCCCAA TCAGTCCAGC TTCGATTGCA TCCTCCAGCA GTACTCCTTC CTCCAAATCC
    AAAGAACTTA GCCTTAACGA AAAATCTACT ACTCCTGTGT CGAAGTCCAA TACCCCTACT
    CCACGAACTG ATGCACCCAC CCCAGGCAGT AATTCCACTC CCGGATTGAG GCCGGTTCCC
    GGAAAACCGC CAGGAGTCGA CCCGTTGGCT TCAAGCCTAA GGACTCCCAT GGCAGTACCT
    TGTCCATACC CAACCCCGTT TGGGATTGTG CCACACGCGG GGATGAATGG GGAGCTGACC
    AGCCCCGGAG CTGCCTACGC AGGGCTACAT AACATCTCCC CACAGATGAG CGCGGCTGCT
    GCTGCTGCCG CCGCGGCTGC TGCCTACGGG AGATCACCAG TGGTTGGATT TGATCCACAC
    CATCACATGC GTGTGCCAGC GATACCTCCA AACCTGACAG GCATTCCAGG AGGAAAGCCG
    GCATACTCCT TCCACGTTAC CGCAGATGGT CAGATGCAGC CTGTCCCCTT CCCCCCGGAC
    GCCCTTATCG GCCCCGGCAT TCCTCGACAC GCTCGCCAGA TCAACACCCT AAACCATGGG
    GAGGTGGTGT GTGCGGTGAC CATCAGCAAC CCCACGCGAC ACGTGTACAC GGGTGGGAAG
    GGCTGCGTCA AGGTCTGGGA CATCAGCCAC CCTGGCAACA AGAGCCCCGT CTCTCAGCTC
    GACTGTCTGA ACAGGGATAA CTACATCCGT TCCTGCCGAT TGCTCCCTGA TGGTCGTACC
    CTAATTGTGG GAGGGGAAGC CAGTACGCTG TCCATCTGGG ACCTGGCAGC TCCCACCCCG
    CGCATCAAGG CAGAGCTGAC GTCCTCAGCC CCCGCCTGCT ACGCCCTGGC CATCAGCCCG
    GACTCCAAGG TCTGCTTCTC GTGCTGCAGT GACGGCAACA TTGCTGTGTG GGACCTGCAC
    AACCAGACGC TGGTGAGGCA ATTCCAGGGC CACACGGACG GGGCCAGCTG TATTGACATC
    TCTAATGATG GCACCAAGCT CTGGACGGGT GGCTTGGACA ACACGGTCCG GTCCTGGGAC
    CTTCGTGAGG GGCGGCAGCT GCAGCAGCAC GACTTCACCT CCCAGATCTT TTCTCTGGGC
    TACTGCCCGA CCGGAGAGTG GCTGGCTGTG GGGATGGAAA ACAGCAACGT GGAGGTGTTG
    CATGTCACCA AGCCCGACAA GTACCAGCTC CACCTGCACG AGAGCTGCGT GCTGTCGCTC
    AAGTTTGCCC ACTGTGGCAA ATGGTTTGTC AGCACGGGAA AGGACAACCT TCTGAATGCC
    TGGAGAACAC CTTATGGGGC CAGTATATTC CAGTCCAAAG AATCCTCATC GGTGCTCAGC
    TGTGACATTT CTGTGGATGA CAAATACATT GTCACTGGCT CTGGGGATAA GAAGGCCACA
    GTTTATGAAG TTATTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236993
    Clone ID Related Accession (Same CDS sequence) XM_024995558.1
    Accession Version XM_024995558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2322bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCACCA GCAACAAGTG GTGCAGGCTG TGGAACGGGC CAAGCAGGTG
    ACCATGGCAG AACTGAACGC CATCATTGGG CAGCAACTCC AGGCCCAGCA TCTATCACAT
    GGACATGGTC TGCCAGTGCC TCTGACCCCA CACCCTTCGG GGCTTCAGCC TCCTGCCATT
    CCGCCCATTG GTAGCAGTGC CGGCCTTCTG GCCCTCTCCA GTGCCCTGGG AGGCCAGTCC
    CACCTTCCAA TTAAAGATGA GAAGAAGCAT CATGACAGTG ATCACCAAAG AGACAGAGAC
    TCCATCAAGA GCTCTTCGGT ATCCCCATCA GCCAGTTTCC GAGGTGCTGA GAAGCACAGA
    AACTCCACAG ACTATTCCTC TGATAGCAAA AAGCAGAAAA CTGAAGAAAA GGAGATTGCA
    GCTCGTTATG ACAGCGATGC TGAGAAGAGT GATGACAACT TGGTGGTTGA CGTTTCCAAT
    GAGGATCCAT CATCACCTCG AGGGAGCCCA GCACATTCCC CGAGAGAGAA CGGCCTGGAC
    AAGACACGCC TGCTCAAGAA AGATGCCCCA ATCAGTCCAG CTTCGATTGC ATCCTCCAGC
    AGTACTCCTT CCTCCAAATC CAAAGAACTT AGCCTTAACG AAAAATCTAC TACTCCTGTG
    TCGAAGTCCA ATACCCCTAC TCCACGAACT GATGCACCCA CCCCAGGCAG TAATTCCACT
    CCCGGATTGA GGCCGGTTCC CGGAAAACCG CCAGGAGTCG ACCCGTTGGC TTCAAGCCTA
    AGGACTCCCA TGGCAGTACC TTGTCCATAC CCAACCCCGT TTGGGATTGT GCCACACGCG
    GGGATGAATG GGGAGCTGAC CAGCCCCGGA GCTGCCTACG CAGGGCTACA TAACATCTCC
    CCACAGATGA GCGCGGCTGC TGCTGCTGCC GCCGCGGCTG CTGCCTACGG GAGATCACCA
    GTGGTTGGAT TTGATCCACA CCATCACATG CGTGTGCCAG CGATACCTCC AAACCTGACA
    GGCATTCCAG GAGGAAAGCC GGCATACTCC TTCCACGTTA CCGCAGATGG TCAGATGCAG
    CCTGTCCCCT TCCCCCCGGA CGCCCTTATC GGCCCCGGCA TTCCTCGACA CGCTCGCCAG
    ATCAACACCC TAAACCATGG GGAGGTGGTG TGTGCGGTGA CCATCAGCAA CCCCACGCGA
    CACGTGTACA CGGGTGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAC
    AAGAGCCCCG TCTCTCAGCT CGACTGTCTG AACAGGGATA ACTACATCCG TTCCTGCCGA
    TTGCTCCCTG ATGGTCGTAC CCTAATTGTG GGAGGGGAAG CCAGTACGCT GTCCATCTGG
    GACCTGGCAG CTCCCACCCC GCGCATCAAG GCAGAGCTGA CGTCCTCAGC CCCCGCCTGC
    TACGCCCTGG CCATCAGCCC GGACTCCAAG GTCTGCTTCT CGTGCTGCAG TGACGGCAAC
    ATTGCTGTGT GGGACCTGCA CAACCAGACG CTGGTGAGGC AATTCCAGGG CCACACGGAC
    GGGGCCAGCT GTATTGACAT CTCTAATGAT GGCACCAAGC TCTGGACGGG TGGCTTGGAC
    AACACGGTCC GGTCCTGGGA CCTTCGTGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
    TCCCAGATCT TTTCTCTGGG CTACTGCCCG ACCGGAGAGT GGCTGGCTGT GGGGATGGAA
    AACAGCAACG TGGAGGTGTT GCATGTCACC AAGCCCGACA AGTACCAGCT CCACCTGCAC
    GAGAGCTGCG TGCTGTCGCT CAAGTTTGCC CACTGTGGCA AATGGTTTGT CAGCACGGGA
    AAGGACAACC TTCTGAATGC CTGGAGAACA CCTTATGGGG CCAGTATATT CCAGTCCAAA
    GAATCCTCAT CGGTGCTCAG CTGTGACATT TCTGTGGATG ACAAATACAT TGTCACTGGC
    TCTGGGGATA AGAAGGCCAC AGTTTATGAA GTTATTTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851326.1
    CDS882..3203
    Translation

    Target ORF information:

    RefSeq Version XM_024995558.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCACCA GCAACAAGTG GTGCAGGCTG TGGAACGGGC CAAGCAGGTG
    ACCATGGCAG AACTGAACGC CATCATTGGG CAGCAACTCC AGGCCCAGCA TCTATCACAT
    GGACATGGTC TGCCAGTGCC TCTGACCCCA CACCCTTCGG GGCTTCAGCC TCCTGCCATT
    CCGCCCATTG GTAGCAGTGC CGGCCTTCTG GCCCTCTCCA GTGCCCTGGG AGGCCAGTCC
    CACCTTCCAA TTAAAGATGA GAAGAAGCAT CATGACAGTG ATCACCAAAG AGACAGAGAC
    TCCATCAAGA GCTCTTCGGT ATCCCCATCA GCCAGTTTCC GAGGTGCTGA GAAGCACAGA
    AACTCCACAG ACTATTCCTC TGATAGCAAA AAGCAGAAAA CTGAAGAAAA GGAGATTGCA
    GCTCGTTATG ACAGCGATGC TGAGAAGAGT GATGACAACT TGGTGGTTGA CGTTTCCAAT
    GAGGATCCAT CATCACCTCG AGGGAGCCCA GCACATTCCC CGAGAGAGAA CGGCCTGGAC
    AAGACACGCC TGCTCAAGAA AGATGCCCCA ATCAGTCCAG CTTCGATTGC ATCCTCCAGC
    AGTACTCCTT CCTCCAAATC CAAAGAACTT AGCCTTAACG AAAAATCTAC TACTCCTGTG
    TCGAAGTCCA ATACCCCTAC TCCACGAACT GATGCACCCA CCCCAGGCAG TAATTCCACT
    CCCGGATTGA GGCCGGTTCC CGGAAAACCG CCAGGAGTCG ACCCGTTGGC TTCAAGCCTA
    AGGACTCCCA TGGCAGTACC TTGTCCATAC CCAACCCCGT TTGGGATTGT GCCACACGCG
    GGGATGAATG GGGAGCTGAC CAGCCCCGGA GCTGCCTACG CAGGGCTACA TAACATCTCC
    CCACAGATGA GCGCGGCTGC TGCTGCTGCC GCCGCGGCTG CTGCCTACGG GAGATCACCA
    GTGGTTGGAT TTGATCCACA CCATCACATG CGTGTGCCAG CGATACCTCC AAACCTGACA
    GGCATTCCAG GAGGAAAGCC GGCATACTCC TTCCACGTTA CCGCAGATGG TCAGATGCAG
    CCTGTCCCCT TCCCCCCGGA CGCCCTTATC GGCCCCGGCA TTCCTCGACA CGCTCGCCAG
    ATCAACACCC TAAACCATGG GGAGGTGGTG TGTGCGGTGA CCATCAGCAA CCCCACGCGA
    CACGTGTACA CGGGTGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAC
    AAGAGCCCCG TCTCTCAGCT CGACTGTCTG AACAGGGATA ACTACATCCG TTCCTGCCGA
    TTGCTCCCTG ATGGTCGTAC CCTAATTGTG GGAGGGGAAG CCAGTACGCT GTCCATCTGG
    GACCTGGCAG CTCCCACCCC GCGCATCAAG GCAGAGCTGA CGTCCTCAGC CCCCGCCTGC
    TACGCCCTGG CCATCAGCCC GGACTCCAAG GTCTGCTTCT CGTGCTGCAG TGACGGCAAC
    ATTGCTGTGT GGGACCTGCA CAACCAGACG CTGGTGAGGC AATTCCAGGG CCACACGGAC
    GGGGCCAGCT GTATTGACAT CTCTAATGAT GGCACCAAGC TCTGGACGGG TGGCTTGGAC
    AACACGGTCC GGTCCTGGGA CCTTCGTGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
    TCCCAGATCT TTTCTCTGGG CTACTGCCCG ACCGGAGAGT GGCTGGCTGT GGGGATGGAA
    AACAGCAACG TGGAGGTGTT GCATGTCACC AAGCCCGACA AGTACCAGCT CCACCTGCAC
    GAGAGCTGCG TGCTGTCGCT CAAGTTTGCC CACTGTGGCA AATGGTTTGT CAGCACGGGA
    AAGGACAACC TTCTGAATGC CTGGAGAACA CCTTATGGGG CCAGTATATT CCAGTCCAAA
    GAATCCTCAT CGGTGCTCAG CTGTGACATT TCTGTGGATG ACAAATACAT TGTCACTGGC
    TCTGGGGATA AGAAGGCCAC AGTTTATGAA GTTATTTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236994
    Clone ID Related Accession (Same CDS sequence) XM_024995559.1
    Accession Version XM_024995559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2262bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCACCAGCA ACAAGTGGTG CAGGCTGTGG AACGGGCCAA GCAGGTGACC
    ATGGCAGAAC TGAACGCCAT CATTGGGCAG CAGCAACTCC AGGCCCAGCA TCTATCACAT
    GGACATGGTC TGCCAGTGCC TCTGACCCCA CACCCTTCGG GGCTTCAGCC TCCTGCCATT
    CCGCCCATTG GTAGCAGTGC CGGCCTTCTG GCCCTCTCCA GTGCCCTGGG AGGCCAGTCC
    CACCTTCCAA TTAAAGATGA GAAGAAGCAT CATGACAGTG ATCACCAAAG AGACAGAGAC
    TCCATCAAGA GCTCTTCGGT ATCCCCATCA GCCAGTTTCC GAGGTGCTGA GAAGCACAGA
    AACTCCACAG ACTATTCCTC TGATAGCAAA AAGCAGAAAA CTGAAGAAAA GGAGATTGCA
    GCTCGTTATG ACAGCGATGC TGAGAAGAGT GATGACAACT TGGTGGTTGA CGTTTCCAAT
    GAGGATCCAT CATCACCTCG AGGGAGCCCA GCACATTCCC CGAGAGAGAA CGGCCTGGAC
    AAGACACGCC TGCTCAAGAA AGATGCCCCA ATCAGTCCAG CTTCGATTGC ATCCTCCAGC
    AGTACTCCTT CCTCCAAATC CAAAGAACTT AGCCTTAACG AAAAATCTAC TACTCCTGTG
    TCGAAGTCCA ATACCCCTAC TCCACGAACT GATGCACCCA CCCCAGGCAG TAATTCCACT
    CCCGGATTGA GGCCGGTTCC CGGAAAACCG CCAGGAGTCG ACCCGTTGGC TTCAAGCCTA
    AGGACTCCCA TGGCAGTACC TTGTCCATAC CCAACCCCGT TTGGGATTGT GCCACACGCG
    GGGATGAATG GGGAGCTGAC CAGCCCCGGA GCTGCCTACG CAGGGCTACA TAACATCTCC
    CCACAGATGA GCGCGGCTGC TGCTGCTGCC GCCGCGGCTG CTGCCTACGG GAGATCACCA
    GTGGTTGGAT TTGATCCACA CCATCACATG CGTGTGCCAG CGATACCTCC AAACCTGACA
    GGCATTCCAG GAGGAAAGCC GGCATACTCC TTCCACGTTA CCGCAGATGG TCAGATGCAG
    CCTGTCCCCT TCCCCCCGGA CGCCCTTATC GGCCCCGGCA TTCCTCGACA CGCTCGCCAG
    ATCAACACCC TAAACCATGG GGAGGTGGTG TGTGCGGTGA CCATCAGCAA CCCCACGCGA
    CACGTGTACA CGGGTGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAC
    AAGAGCCCCG TCTCTCAGCT CGACTGTCTG AACAGGGATA ACTACATCCG TTCCTGCCGA
    TTGCTCCCTG ATGGTCGTAC CCTAATTGTG GGAGGGGAAG CCAGTACGCT GTCCATCTGG
    GACCTGGCAG CTCCCACCCC GCGCATCAAG GCAGAGCTGA CGTCCTCAGC CCCCGCCTGC
    TACGCCCTGG CCATCAGCCC GGACTCCAAG GTCTGCTTCT CGTGCTGCAG TGACGGCAAC
    ATTGCTGTGT GGGACCTGCA CAACCAGACG CTGGTGAGGC AATTCCAGGG CCACACGGAC
    GGGGCCAGCT GTATTGACAT CTCTAATGAT GGCACCAAGC TCTGGACGGG TGGCTTGGAC
    AACACGGTCC GGTCCTGGGA CCTTCGTGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
    TCCCAGATCT TTTCTCTGGG CTACTGCCCG ACCGGAGAGT GGCTGGCTGT GGGGATGGAA
    AACAGCAACG TGGAGGTGTT GCATGTCACC AAGCCCGACA AGTACCAGCT CCACCTGCAC
    GAGAGCTGCG TGCTGTCGCT CAAGTTTGCC CACTGTGGCA AATGGTTTGT CAGCACGGGA
    AAGGACAACC TTCTGAATGC CTGGAGAACA CCTTATGGGG CCAGTATATT CCAGTCCAAA
    GAATCCTCAT CGGTGCTCAG CTGTGACATT TCTGTGGATG ACAAATACAT TGTCACTGGC
    TCTGGGGATA AGAAGGCCAC AGTTTATGAA GTTATTTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851327.1
    CDS882..3143
    Translation

    Target ORF information:

    RefSeq Version XM_024995559.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCACCAGCA ACAAGTGGTG CAGGCTGTGG AACGGGCCAA GCAGGTGACC
    ATGGCAGAAC TGAACGCCAT CATTGGGCAG CAGCAACTCC AGGCCCAGCA TCTATCACAT
    GGACATGGTC TGCCAGTGCC TCTGACCCCA CACCCTTCGG GGCTTCAGCC TCCTGCCATT
    CCGCCCATTG GTAGCAGTGC CGGCCTTCTG GCCCTCTCCA GTGCCCTGGG AGGCCAGTCC
    CACCTTCCAA TTAAAGATGA GAAGAAGCAT CATGACAGTG ATCACCAAAG AGACAGAGAC
    TCCATCAAGA GCTCTTCGGT ATCCCCATCA GCCAGTTTCC GAGGTGCTGA GAAGCACAGA
    AACTCCACAG ACTATTCCTC TGATAGCAAA AAGCAGAAAA CTGAAGAAAA GGAGATTGCA
    GCTCGTTATG ACAGCGATGC TGAGAAGAGT GATGACAACT TGGTGGTTGA CGTTTCCAAT
    GAGGATCCAT CATCACCTCG AGGGAGCCCA GCACATTCCC CGAGAGAGAA CGGCCTGGAC
    AAGACACGCC TGCTCAAGAA AGATGCCCCA ATCAGTCCAG CTTCGATTGC ATCCTCCAGC
    AGTACTCCTT CCTCCAAATC CAAAGAACTT AGCCTTAACG AAAAATCTAC TACTCCTGTG
    TCGAAGTCCA ATACCCCTAC TCCACGAACT GATGCACCCA CCCCAGGCAG TAATTCCACT
    CCCGGATTGA GGCCGGTTCC CGGAAAACCG CCAGGAGTCG ACCCGTTGGC TTCAAGCCTA
    AGGACTCCCA TGGCAGTACC TTGTCCATAC CCAACCCCGT TTGGGATTGT GCCACACGCG
    GGGATGAATG GGGAGCTGAC CAGCCCCGGA GCTGCCTACG CAGGGCTACA TAACATCTCC
    CCACAGATGA GCGCGGCTGC TGCTGCTGCC GCCGCGGCTG CTGCCTACGG GAGATCACCA
    GTGGTTGGAT TTGATCCACA CCATCACATG CGTGTGCCAG CGATACCTCC AAACCTGACA
    GGCATTCCAG GAGGAAAGCC GGCATACTCC TTCCACGTTA CCGCAGATGG TCAGATGCAG
    CCTGTCCCCT TCCCCCCGGA CGCCCTTATC GGCCCCGGCA TTCCTCGACA CGCTCGCCAG
    ATCAACACCC TAAACCATGG GGAGGTGGTG TGTGCGGTGA CCATCAGCAA CCCCACGCGA
    CACGTGTACA CGGGTGGGAA GGGCTGCGTC AAGGTCTGGG ACATCAGCCA CCCTGGCAAC
    AAGAGCCCCG TCTCTCAGCT CGACTGTCTG AACAGGGATA ACTACATCCG TTCCTGCCGA
    TTGCTCCCTG ATGGTCGTAC CCTAATTGTG GGAGGGGAAG CCAGTACGCT GTCCATCTGG
    GACCTGGCAG CTCCCACCCC GCGCATCAAG GCAGAGCTGA CGTCCTCAGC CCCCGCCTGC
    TACGCCCTGG CCATCAGCCC GGACTCCAAG GTCTGCTTCT CGTGCTGCAG TGACGGCAAC
    ATTGCTGTGT GGGACCTGCA CAACCAGACG CTGGTGAGGC AATTCCAGGG CCACACGGAC
    GGGGCCAGCT GTATTGACAT CTCTAATGAT GGCACCAAGC TCTGGACGGG TGGCTTGGAC
    AACACGGTCC GGTCCTGGGA CCTTCGTGAG GGGCGGCAGC TGCAGCAGCA CGACTTCACC
    TCCCAGATCT TTTCTCTGGG CTACTGCCCG ACCGGAGAGT GGCTGGCTGT GGGGATGGAA
    AACAGCAACG TGGAGGTGTT GCATGTCACC AAGCCCGACA AGTACCAGCT CCACCTGCAC
    GAGAGCTGCG TGCTGTCGCT CAAGTTTGCC CACTGTGGCA AATGGTTTGT CAGCACGGGA
    AAGGACAACC TTCTGAATGC CTGGAGAACA CCTTATGGGG CCAGTATATT CCAGTCCAAA
    GAATCCTCAT CGGTGCTCAG CTGTGACATT TCTGTGGATG ACAAATACAT TGTCACTGGC
    TCTGGGGATA AGAAGGCCAC AGTTTATGAA GTTATTTATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236995
    Clone ID Related Accession (Same CDS sequence) XM_024995560.1
    Accession Version XM_024995560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCACCAGCA ACAAGTGGTG CAGGCTGTGG AACGGGCCAA GCAGGTGACC
    ATGGCAGAAC TGAACGCCAT CATTGGGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851328.1
    CDS882..3140
    Translation

    Target ORF information:

    RefSeq Version XM_024995560.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCACCAGCA ACAAGTGGTG CAGGCTGTGG AACGGGCCAA GCAGGTGACC
    ATGGCAGAAC TGAACGCCAT CATTGGGCAG CAACTCCAGG CCCAGCATCT ATCACATGGA
    CATGGTCTGC CAGTGCCTCT GACCCCACAC CCTTCGGGGC TTCAGCCTCC TGCCATTCCG
    CCCATTGGTA GCAGTGCCGG CCTTCTGGCC CTCTCCAGTG CCCTGGGAGG CCAGTCCCAC
    CTTCCAATTA AAGATGAGAA GAAGCATCAT GACAGTGATC ACCAAAGAGA CAGAGACTCC
    ATCAAGAGCT CTTCGGTATC CCCATCAGCC AGTTTCCGAG GTGCTGAGAA GCACAGAAAC
    TCCACAGACT ATTCCTCTGA TAGCAAAAAG CAGAAAACTG AAGAAAAGGA GATTGCAGCT
    CGTTATGACA GCGATGCTGA GAAGAGTGAT GACAACTTGG TGGTTGACGT TTCCAATGAG
    GATCCATCAT CACCTCGAGG GAGCCCAGCA CATTCCCCGA GAGAGAACGG CCTGGACAAG
    ACACGCCTGC TCAAGAAAGA TGCCCCAATC AGTCCAGCTT CGATTGCATC CTCCAGCAGT
    ACTCCTTCCT CCAAATCCAA AGAACTTAGC CTTAACGAAA AATCTACTAC TCCTGTGTCG
    AAGTCCAATA CCCCTACTCC ACGAACTGAT GCACCCACCC CAGGCAGTAA TTCCACTCCC
    GGATTGAGGC CGGTTCCCGG AAAACCGCCA GGAGTCGACC CGTTGGCTTC AAGCCTAAGG
    ACTCCCATGG CAGTACCTTG TCCATACCCA ACCCCGTTTG GGATTGTGCC ACACGCGGGG
    ATGAATGGGG AGCTGACCAG CCCCGGAGCT GCCTACGCAG GGCTACATAA CATCTCCCCA
    CAGATGAGCG CGGCTGCTGC TGCTGCCGCC GCGGCTGCTG CCTACGGGAG ATCACCAGTG
    GTTGGATTTG ATCCACACCA TCACATGCGT GTGCCAGCGA TACCTCCAAA CCTGACAGGC
    ATTCCAGGAG GAAAGCCGGC ATACTCCTTC CACGTTACCG CAGATGGTCA GATGCAGCCT
    GTCCCCTTCC CCCCGGACGC CCTTATCGGC CCCGGCATTC CTCGACACGC TCGCCAGATC
    AACACCCTAA ACCATGGGGA GGTGGTGTGT GCGGTGACCA TCAGCAACCC CACGCGACAC
    GTGTACACGG GTGGGAAGGG CTGCGTCAAG GTCTGGGACA TCAGCCACCC TGGCAACAAG
    AGCCCCGTCT CTCAGCTCGA CTGTCTGAAC AGGGATAACT ACATCCGTTC CTGCCGATTG
    CTCCCTGATG GTCGTACCCT AATTGTGGGA GGGGAAGCCA GTACGCTGTC CATCTGGGAC
    CTGGCAGCTC CCACCCCGCG CATCAAGGCA GAGCTGACGT CCTCAGCCCC CGCCTGCTAC
    GCCCTGGCCA TCAGCCCGGA CTCCAAGGTC TGCTTCTCGT GCTGCAGTGA CGGCAACATT
    GCTGTGTGGG ACCTGCACAA CCAGACGCTG GTGAGGCAAT TCCAGGGCCA CACGGACGGG
    GCCAGCTGTA TTGACATCTC TAATGATGGC ACCAAGCTCT GGACGGGTGG CTTGGACAAC
    ACGGTCCGGT CCTGGGACCT TCGTGAGGGG CGGCAGCTGC AGCAGCACGA CTTCACCTCC
    CAGATCTTTT CTCTGGGCTA CTGCCCGACC GGAGAGTGGC TGGCTGTGGG GATGGAAAAC
    AGCAACGTGG AGGTGTTGCA TGTCACCAAG CCCGACAAGT ACCAGCTCCA CCTGCACGAG
    AGCTGCGTGC TGTCGCTCAA GTTTGCCCAC TGTGGCAAAT GGTTTGTCAG CACGGGAAAG
    GACAACCTTC TGAATGCCTG GAGAACACCT TATGGGGCCA GTATATTCCA GTCCAAAGAA
    TCCTCATCGG TGCTCAGCTG TGACATTTCT GTGGATGACA AATACATTGT CACTGGCTCT
    GGGGATAAGA AGGCCACAGT TTATGAAGTT ATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236996
    Clone ID Related Accession (Same CDS sequence) XM_024995561.1
    Accession Version XM_024995561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2253bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCACC AGCAACAAGT GGTGCAGGCT GTGGAACGGG CCAAGCAGGT GACCATGGCA
    GAACTGAACG CCATCATTGG GCAGCAACTC CAGGCCCAGC ATCTATCACA TGGACATGGT
    CTGCCAGTGC CTCTGACCCC ACACCCTTCG GGGCTTCAGC CTCCTGCCAT TCCGCCCATT
    GGTAGCAGTG CCGGCCTTCT GGCCCTCTCC AGTGCCCTGG GAGGCCAGTC CCACCTTCCA
    ATTAAAGATG AGAAGAAGCA TCATGACAGT GATCACCAAA GAGACAGAGA CTCCATCAAG
    AGCTCTTCGG TATCCCCATC AGCCAGTTTC CGAGGTGCTG AGAAGCACAG AAACTCCACA
    GACTATTCCT CTGATAGCAA AAAGCAGAAA ACTGAAGAAA AGGAGATTGC AGCTCGTTAT
    GACAGCGATG CTGAGAAGAG TGATGACAAC TTGGTGGTTG ACGTTTCCAA TGAGGATCCA
    TCATCACCTC GAGGGAGCCC AGCACATTCC CCGAGAGAGA ACGGCCTGGA CAAGACACGC
    CTGCTCAAGA AAGATGCCCC AATCAGTCCA GCTTCGATTG CATCCTCCAG CAGTACTCCT
    TCCTCCAAAT CCAAAGAACT TAGCCTTAAC GAAAAATCTA CTACTCCTGT GTCGAAGTCC
    AATACCCCTA CTCCACGAAC TGATGCACCC ACCCCAGGCA GTAATTCCAC TCCCGGATTG
    AGGCCGGTTC CCGGAAAACC GCCAGGAGTC GACCCGTTGG CTTCAAGCCT AAGGACTCCC
    ATGGCAGTAC CTTGTCCATA CCCAACCCCG TTTGGGATTG TGCCACACGC GGGGATGAAT
    GGGGAGCTGA CCAGCCCCGG AGCTGCCTAC GCAGGGCTAC ATAACATCTC CCCACAGATG
    AGCGCGGCTG CTGCTGCTGC CGCCGCGGCT GCTGCCTACG GGAGATCACC AGTGGTTGGA
    TTTGATCCAC ACCATCACAT GCGTGTGCCA GCGATACCTC CAAACCTGAC AGGCATTCCA
    GGAGGAAAGC CGGCATACTC CTTCCACGTT ACCGCAGATG GTCAGATGCA GCCTGTCCCC
    TTCCCCCCGG ACGCCCTTAT CGGCCCCGGC ATTCCTCGAC ACGCTCGCCA GATCAACACC
    CTAAACCATG GGGAGGTGGT GTGTGCGGTG ACCATCAGCA ACCCCACGCG ACACGTGTAC
    ACGGGTGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA CAAGAGCCCC
    GTCTCTCAGC TCGACTGTCT GAACAGGGAT AACTACATCC GTTCCTGCCG ATTGCTCCCT
    GATGGTCGTA CCCTAATTGT GGGAGGGGAA GCCAGTACGC TGTCCATCTG GGACCTGGCA
    GCTCCCACCC CGCGCATCAA GGCAGAGCTG ACGTCCTCAG CCCCCGCCTG CTACGCCCTG
    GCCATCAGCC CGGACTCCAA GGTCTGCTTC TCGTGCTGCA GTGACGGCAA CATTGCTGTG
    TGGGACCTGC ACAACCAGAC GCTGGTGAGG CAATTCCAGG GCCACACGGA CGGGGCCAGC
    TGTATTGACA TCTCTAATGA TGGCACCAAG CTCTGGACGG GTGGCTTGGA CAACACGGTC
    CGGTCCTGGG ACCTTCGTGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
    TTTTCTCTGG GCTACTGCCC GACCGGAGAG TGGCTGGCTG TGGGGATGGA AAACAGCAAC
    GTGGAGGTGT TGCATGTCAC CAAGCCCGAC AAGTACCAGC TCCACCTGCA CGAGAGCTGC
    GTGCTGTCGC TCAAGTTTGC CCACTGTGGC AAATGGTTTG TCAGCACGGG AAAGGACAAC
    CTTCTGAATG CCTGGAGAAC ACCTTATGGG GCCAGTATAT TCCAGTCCAA AGAATCCTCA
    TCGGTGCTCA GCTGTGACAT TTCTGTGGAT GACAAATACA TTGTCACTGG CTCTGGGGAT
    AAGAAGGCCA CAGTTTATGA AGTTATTTAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851329.1
    CDS113..2365
    Translation

    Target ORF information:

    RefSeq Version XM_024995561.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGGCGCGCG TCCCCCCGAC GGGCCAGTTT CGATTCCGGG CACCGCATCA GCCTGCTCAA 
    CCCTTTAAAT TTACAATTTC CGAGTCCTGT GATCGGATTA AGGAAGAGTT TCAGTTTTTA
    CAGGCTCAGT ACCACAGCCT GAAACTGGAA TGTGAGAAAC TCGCCAGTGA GAAGACAGAG
    ATGCAGCGGC ATTATGTGAT GTATTATGAG ATGTCCTATG GGTTGAATAT AGAAATGCAC
    AAGCAGCACC AGCAACAAGT GGTGCAGGCT GTGGAACGGG CCAAGCAGGT GACCATGGCA
    GAACTGAACG CCATCATTGG GCAGCAACTC CAGGCCCAGC ATCTATCACA TGGACATGGT
    CTGCCAGTGC CTCTGACCCC ACACCCTTCG GGGCTTCAGC CTCCTGCCAT TCCGCCCATT
    GGTAGCAGTG CCGGCCTTCT GGCCCTCTCC AGTGCCCTGG GAGGCCAGTC CCACCTTCCA
    ATTAAAGATG AGAAGAAGCA TCATGACAGT GATCACCAAA GAGACAGAGA CTCCATCAAG
    AGCTCTTCGG TATCCCCATC AGCCAGTTTC CGAGGTGCTG AGAAGCACAG AAACTCCACA
    GACTATTCCT CTGATAGCAA AAAGCAGAAA ACTGAAGAAA AGGAGATTGC AGCTCGTTAT
    GACAGCGATG CTGAGAAGAG TGATGACAAC TTGGTGGTTG ACGTTTCCAA TGAGGATCCA
    TCATCACCTC GAGGGAGCCC AGCACATTCC CCGAGAGAGA ACGGCCTGGA CAAGACACGC
    CTGCTCAAGA AAGATGCCCC AATCAGTCCA GCTTCGATTG CATCCTCCAG CAGTACTCCT
    TCCTCCAAAT CCAAAGAACT TAGCCTTAAC GAAAAATCTA CTACTCCTGT GTCGAAGTCC
    AATACCCCTA CTCCACGAAC TGATGCACCC ACCCCAGGCA GTAATTCCAC TCCCGGATTG
    AGGCCGGTTC CCGGAAAACC GCCAGGAGTC GACCCGTTGG CTTCAAGCCT AAGGACTCCC
    ATGGCAGTAC CTTGTCCATA CCCAACCCCG TTTGGGATTG TGCCACACGC GGGGATGAAT
    GGGGAGCTGA CCAGCCCCGG AGCTGCCTAC GCAGGGCTAC ATAACATCTC CCCACAGATG
    AGCGCGGCTG CTGCTGCTGC CGCCGCGGCT GCTGCCTACG GGAGATCACC AGTGGTTGGA
    TTTGATCCAC ACCATCACAT GCGTGTGCCA GCGATACCTC CAAACCTGAC AGGCATTCCA
    GGAGGAAAGC CGGCATACTC CTTCCACGTT ACCGCAGATG GTCAGATGCA GCCTGTCCCC
    TTCCCCCCGG ACGCCCTTAT CGGCCCCGGC ATTCCTCGAC ACGCTCGCCA GATCAACACC
    CTAAACCATG GGGAGGTGGT GTGTGCGGTG ACCATCAGCA ACCCCACGCG ACACGTGTAC
    ACGGGTGGGA AGGGCTGCGT CAAGGTCTGG GACATCAGCC ACCCTGGCAA CAAGAGCCCC
    GTCTCTCAGC TCGACTGTCT GAACAGGGAT AACTACATCC GTTCCTGCCG ATTGCTCCCT
    GATGGTCGTA CCCTAATTGT GGGAGGGGAA GCCAGTACGC TGTCCATCTG GGACCTGGCA
    GCTCCCACCC CGCGCATCAA GGCAGAGCTG ACGTCCTCAG CCCCCGCCTG CTACGCCCTG
    GCCATCAGCC CGGACTCCAA GGTCTGCTTC TCGTGCTGCA GTGACGGCAA CATTGCTGTG
    TGGGACCTGC ACAACCAGAC GCTGGTGAGG CAATTCCAGG GCCACACGGA CGGGGCCAGC
    TGTATTGACA TCTCTAATGA TGGCACCAAG CTCTGGACGG GTGGCTTGGA CAACACGGTC
    CGGTCCTGGG ACCTTCGTGA GGGGCGGCAG CTGCAGCAGC ACGACTTCAC CTCCCAGATC
    TTTTCTCTGG GCTACTGCCC GACCGGAGAG TGGCTGGCTG TGGGGATGGA AAACAGCAAC
    GTGGAGGTGT TGCATGTCAC CAAGCCCGAC AAGTACCAGC TCCACCTGCA CGAGAGCTGC
    GTGCTGTCGC TCAAGTTTGC CCACTGTGGC AAATGGTTTG TCAGCACGGG AAAGGACAAC
    CTTCTGAATG CCTGGAGAAC ACCTTATGGG GCCAGTATAT TCCAGTCCAA AGAATCCTCA
    TCGGTGCTCA GCTGTGACAT TTCTGTGGAT GACAAATACA TTGTCACTGG CTCTGGGGAT
    AAGAAGGCCA CAGTTTATGA AGTTATTTAT TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236997
    Clone ID Related Accession (Same CDS sequence) XM_024995562.1
    Accession Version XM_024995562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2250bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCAGCA GCAACTCCAG GCCCAGCATC TATCACATGG ACATGGTCTG
    CCAGTGCCTC TGACCCCACA CCCTTCGGGG CTTCAGCCTC CTGCCATTCC GCCCATTGGT
    AGCAGTGCCG GCCTTCTGGC CCTCTCCAGT GCCCTGGGAG GCCAGTCCCA CCTTCCAATT
    AAAGATGAGA AGAAGCATCA TGACAGTGAT CACCAAAGAG ACAGAGACTC CATCAAGAGC
    TCTTCGGTAT CCCCATCAGC CAGTTTCCGA GGTGCTGAGA AGCACAGAAA CTCCACAGAC
    TATTCCTCTG ATAGCAAAAA GCAGAAAACT GAAGAAAAGG AGATTGCAGC TCGTTATGAC
    AGCGATGCTG AGAAGAGTGA TGACAACTTG GTGGTTGACG TTTCCAATGA GGATCCATCA
    TCACCTCGAG GGAGCCCAGC ACATTCCCCG AGAGAGAACG GCCTGGACAA GACACGCCTG
    CTCAAGAAAG ATGCCCCAAT CAGTCCAGCT TCGATTGCAT CCTCCAGCAG TACTCCTTCC
    TCCAAATCCA AAGAACTTAG CCTTAACGAA AAATCTACTA CTCCTGTGTC GAAGTCCAAT
    ACCCCTACTC CACGAACTGA TGCACCCACC CCAGGCAGTA ATTCCACTCC CGGATTGAGG
    CCGGTTCCCG GAAAACCGCC AGGAGTCGAC CCGTTGGCTT CAAGCCTAAG GACTCCCATG
    GCAGTACCTT GTCCATACCC AACCCCGTTT GGGATTGTGC CACACGCGGG GATGAATGGG
    GAGCTGACCA GCCCCGGAGC TGCCTACGCA GGGCTACATA ACATCTCCCC ACAGATGAGC
    GCGGCTGCTG CTGCTGCCGC CGCGGCTGCT GCCTACGGGA GATCACCAGT GGTTGGATTT
    GATCCACACC ATCACATGCG TGTGCCAGCG ATACCTCCAA ACCTGACAGG CATTCCAGGA
    GGAAAGCCGG CATACTCCTT CCACGTTACC GCAGATGGTC AGATGCAGCC TGTCCCCTTC
    CCCCCGGACG CCCTTATCGG CCCCGGCATT CCTCGACACG CTCGCCAGAT CAACACCCTA
    AACCATGGGG AGGTGGTGTG TGCGGTGACC ATCAGCAACC CCACGCGACA CGTGTACACG
    GGTGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAACAA GAGCCCCGTC
    TCTCAGCTCG ACTGTCTGAA CAGGGATAAC TACATCCGTT CCTGCCGATT GCTCCCTGAT
    GGTCGTACCC TAATTGTGGG AGGGGAAGCC AGTACGCTGT CCATCTGGGA CCTGGCAGCT
    CCCACCCCGC GCATCAAGGC AGAGCTGACG TCCTCAGCCC CCGCCTGCTA CGCCCTGGCC
    ATCAGCCCGG ACTCCAAGGT CTGCTTCTCG TGCTGCAGTG ACGGCAACAT TGCTGTGTGG
    GACCTGCACA ACCAGACGCT GGTGAGGCAA TTCCAGGGCC ACACGGACGG GGCCAGCTGT
    ATTGACATCT CTAATGATGG CACCAAGCTC TGGACGGGTG GCTTGGACAA CACGGTCCGG
    TCCTGGGACC TTCGTGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTT
    TCTCTGGGCT ACTGCCCGAC CGGAGAGTGG CTGGCTGTGG GGATGGAAAA CAGCAACGTG
    GAGGTGTTGC ATGTCACCAA GCCCGACAAG TACCAGCTCC ACCTGCACGA GAGCTGCGTG
    CTGTCGCTCA AGTTTGCCCA CTGTGGCAAA TGGTTTGTCA GCACGGGAAA GGACAACCTT
    CTGAATGCCT GGAGAACACC TTATGGGGCC AGTATATTCC AGTCCAAAGA ATCCTCATCG
    GTGCTCAGCT GTGACATTTC TGTGGATGAC AAATACATTG TCACTGGCTC TGGGGATAAG
    AAGGCCACAG TTTATGAAGT TATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851330.1
    CDS882..3131
    Translation

    Target ORF information:

    RefSeq Version XM_024995562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCAGCA GCAACTCCAG GCCCAGCATC TATCACATGG ACATGGTCTG
    CCAGTGCCTC TGACCCCACA CCCTTCGGGG CTTCAGCCTC CTGCCATTCC GCCCATTGGT
    AGCAGTGCCG GCCTTCTGGC CCTCTCCAGT GCCCTGGGAG GCCAGTCCCA CCTTCCAATT
    AAAGATGAGA AGAAGCATCA TGACAGTGAT CACCAAAGAG ACAGAGACTC CATCAAGAGC
    TCTTCGGTAT CCCCATCAGC CAGTTTCCGA GGTGCTGAGA AGCACAGAAA CTCCACAGAC
    TATTCCTCTG ATAGCAAAAA GCAGAAAACT GAAGAAAAGG AGATTGCAGC TCGTTATGAC
    AGCGATGCTG AGAAGAGTGA TGACAACTTG GTGGTTGACG TTTCCAATGA GGATCCATCA
    TCACCTCGAG GGAGCCCAGC ACATTCCCCG AGAGAGAACG GCCTGGACAA GACACGCCTG
    CTCAAGAAAG ATGCCCCAAT CAGTCCAGCT TCGATTGCAT CCTCCAGCAG TACTCCTTCC
    TCCAAATCCA AAGAACTTAG CCTTAACGAA AAATCTACTA CTCCTGTGTC GAAGTCCAAT
    ACCCCTACTC CACGAACTGA TGCACCCACC CCAGGCAGTA ATTCCACTCC CGGATTGAGG
    CCGGTTCCCG GAAAACCGCC AGGAGTCGAC CCGTTGGCTT CAAGCCTAAG GACTCCCATG
    GCAGTACCTT GTCCATACCC AACCCCGTTT GGGATTGTGC CACACGCGGG GATGAATGGG
    GAGCTGACCA GCCCCGGAGC TGCCTACGCA GGGCTACATA ACATCTCCCC ACAGATGAGC
    GCGGCTGCTG CTGCTGCCGC CGCGGCTGCT GCCTACGGGA GATCACCAGT GGTTGGATTT
    GATCCACACC ATCACATGCG TGTGCCAGCG ATACCTCCAA ACCTGACAGG CATTCCAGGA
    GGAAAGCCGG CATACTCCTT CCACGTTACC GCAGATGGTC AGATGCAGCC TGTCCCCTTC
    CCCCCGGACG CCCTTATCGG CCCCGGCATT CCTCGACACG CTCGCCAGAT CAACACCCTA
    AACCATGGGG AGGTGGTGTG TGCGGTGACC ATCAGCAACC CCACGCGACA CGTGTACACG
    GGTGGGAAGG GCTGCGTCAA GGTCTGGGAC ATCAGCCACC CTGGCAACAA GAGCCCCGTC
    TCTCAGCTCG ACTGTCTGAA CAGGGATAAC TACATCCGTT CCTGCCGATT GCTCCCTGAT
    GGTCGTACCC TAATTGTGGG AGGGGAAGCC AGTACGCTGT CCATCTGGGA CCTGGCAGCT
    CCCACCCCGC GCATCAAGGC AGAGCTGACG TCCTCAGCCC CCGCCTGCTA CGCCCTGGCC
    ATCAGCCCGG ACTCCAAGGT CTGCTTCTCG TGCTGCAGTG ACGGCAACAT TGCTGTGTGG
    GACCTGCACA ACCAGACGCT GGTGAGGCAA TTCCAGGGCC ACACGGACGG GGCCAGCTGT
    ATTGACATCT CTAATGATGG CACCAAGCTC TGGACGGGTG GCTTGGACAA CACGGTCCGG
    TCCTGGGACC TTCGTGAGGG GCGGCAGCTG CAGCAGCACG ACTTCACCTC CCAGATCTTT
    TCTCTGGGCT ACTGCCCGAC CGGAGAGTGG CTGGCTGTGG GGATGGAAAA CAGCAACGTG
    GAGGTGTTGC ATGTCACCAA GCCCGACAAG TACCAGCTCC ACCTGCACGA GAGCTGCGTG
    CTGTCGCTCA AGTTTGCCCA CTGTGGCAAA TGGTTTGTCA GCACGGGAAA GGACAACCTT
    CTGAATGCCT GGAGAACACC TTATGGGGCC AGTATATTCC AGTCCAAAGA ATCCTCATCG
    GTGCTCAGCT GTGACATTTC TGTGGATGAC AAATACATTG TCACTGGCTC TGGGGATAAG
    AAGGCCACAG TTTATGAAGT TATTTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236998
    Clone ID Related Accession (Same CDS sequence) XM_024995563.1
    Accession Version XM_024995563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCAGCA ACTCCAGGCC CAGCATCTAT CACATGGACA TGGTCTGCCA
    GTGCCTCTGA CCCCACACCC TTCGGGGCTT CAGCCTCCTG CCATTCCGCC CATTGGTAGC
    AGTGCCGGCC TTCTGGCCCT CTCCAGTGCC CTGGGAGGCC AGTCCCACCT TCCAATTAAA
    GATGAGAAGA AGCATCATGA CAGTGATCAC CAAAGAGACA GAGACTCCAT CAAGAGCTCT
    TCGGTATCCC CATCAGCCAG TTTCCGAGGT GCTGAGAAGC ACAGAAACTC CACAGACTAT
    TCCTCTGATA GCAAAAAGCA GAAAACTGAA GAAAAGGAGA TTGCAGCTCG TTATGACAGC
    GATGCTGAGA AGAGTGATGA CAACTTGGTG GTTGACGTTT CCAATGAGGA TCCATCATCA
    CCTCGAGGGA GCCCAGCACA TTCCCCGAGA GAGAACGGCC TGGACAAGAC ACGCCTGCTC
    AAGAAAGATG CCCCAATCAG TCCAGCTTCG ATTGCATCCT CCAGCAGTAC TCCTTCCTCC
    AAATCCAAAG AACTTAGCCT TAACGAAAAA TCTACTACTC CTGTGTCGAA GTCCAATACC
    CCTACTCCAC GAACTGATGC ACCCACCCCA GGCAGTAATT CCACTCCCGG ATTGAGGCCG
    GTTCCCGGAA AACCGCCAGG AGTCGACCCG TTGGCTTCAA GCCTAAGGAC TCCCATGGCA
    GTACCTTGTC CATACCCAAC CCCGTTTGGG ATTGTGCCAC ACGCGGGGAT GAATGGGGAG
    CTGACCAGCC CCGGAGCTGC CTACGCAGGG CTACATAACA TCTCCCCACA GATGAGCGCG
    GCTGCTGCTG CTGCCGCCGC GGCTGCTGCC TACGGGAGAT CACCAGTGGT TGGATTTGAT
    CCACACCATC ACATGCGTGT GCCAGCGATA CCTCCAAACC TGACAGGCAT TCCAGGAGGA
    AAGCCGGCAT ACTCCTTCCA CGTTACCGCA GATGGTCAGA TGCAGCCTGT CCCCTTCCCC
    CCGGACGCCC TTATCGGCCC CGGCATTCCT CGACACGCTC GCCAGATCAA CACCCTAAAC
    CATGGGGAGG TGGTGTGTGC GGTGACCATC AGCAACCCCA CGCGACACGT GTACACGGGT
    GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAACAAGAG CCCCGTCTCT
    CAGCTCGACT GTCTGAACAG GGATAACTAC ATCCGTTCCT GCCGATTGCT CCCTGATGGT
    CGTACCCTAA TTGTGGGAGG GGAAGCCAGT ACGCTGTCCA TCTGGGACCT GGCAGCTCCC
    ACCCCGCGCA TCAAGGCAGA GCTGACGTCC TCAGCCCCCG CCTGCTACGC CCTGGCCATC
    AGCCCGGACT CCAAGGTCTG CTTCTCGTGC TGCAGTGACG GCAACATTGC TGTGTGGGAC
    CTGCACAACC AGACGCTGGT GAGGCAATTC CAGGGCCACA CGGACGGGGC CAGCTGTATT
    GACATCTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGCT TGGACAACAC GGTCCGGTCC
    TGGGACCTTC GTGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTTTCT
    CTGGGCTACT GCCCGACCGG AGAGTGGCTG GCTGTGGGGA TGGAAAACAG CAACGTGGAG
    GTGTTGCATG TCACCAAGCC CGACAAGTAC CAGCTCCACC TGCACGAGAG CTGCGTGCTG
    TCGCTCAAGT TTGCCCACTG TGGCAAATGG TTTGTCAGCA CGGGAAAGGA CAACCTTCTG
    AATGCCTGGA GAACACCTTA TGGGGCCAGT ATATTCCAGT CCAAAGAATC CTCATCGGTG
    CTCAGCTGTG ACATTTCTGT GGATGACAAA TACATTGTCA CTGGCTCTGG GGATAAGAAG
    GCCACAGTTT ATGAAGTTAT TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851331.1
    CDS882..3128
    Translation

    Target ORF information:

    RefSeq Version XM_024995563.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGGCAGAGAT TGTCAAGAGG CTGAACGCTA TCTGTGCACA AGTCATTCCT
    TTCCTGTCCC AAGAGCAGCA ACTCCAGGCC CAGCATCTAT CACATGGACA TGGTCTGCCA
    GTGCCTCTGA CCCCACACCC TTCGGGGCTT CAGCCTCCTG CCATTCCGCC CATTGGTAGC
    AGTGCCGGCC TTCTGGCCCT CTCCAGTGCC CTGGGAGGCC AGTCCCACCT TCCAATTAAA
    GATGAGAAGA AGCATCATGA CAGTGATCAC CAAAGAGACA GAGACTCCAT CAAGAGCTCT
    TCGGTATCCC CATCAGCCAG TTTCCGAGGT GCTGAGAAGC ACAGAAACTC CACAGACTAT
    TCCTCTGATA GCAAAAAGCA GAAAACTGAA GAAAAGGAGA TTGCAGCTCG TTATGACAGC
    GATGCTGAGA AGAGTGATGA CAACTTGGTG GTTGACGTTT CCAATGAGGA TCCATCATCA
    CCTCGAGGGA GCCCAGCACA TTCCCCGAGA GAGAACGGCC TGGACAAGAC ACGCCTGCTC
    AAGAAAGATG CCCCAATCAG TCCAGCTTCG ATTGCATCCT CCAGCAGTAC TCCTTCCTCC
    AAATCCAAAG AACTTAGCCT TAACGAAAAA TCTACTACTC CTGTGTCGAA GTCCAATACC
    CCTACTCCAC GAACTGATGC ACCCACCCCA GGCAGTAATT CCACTCCCGG ATTGAGGCCG
    GTTCCCGGAA AACCGCCAGG AGTCGACCCG TTGGCTTCAA GCCTAAGGAC TCCCATGGCA
    GTACCTTGTC CATACCCAAC CCCGTTTGGG ATTGTGCCAC ACGCGGGGAT GAATGGGGAG
    CTGACCAGCC CCGGAGCTGC CTACGCAGGG CTACATAACA TCTCCCCACA GATGAGCGCG
    GCTGCTGCTG CTGCCGCCGC GGCTGCTGCC TACGGGAGAT CACCAGTGGT TGGATTTGAT
    CCACACCATC ACATGCGTGT GCCAGCGATA CCTCCAAACC TGACAGGCAT TCCAGGAGGA
    AAGCCGGCAT ACTCCTTCCA CGTTACCGCA GATGGTCAGA TGCAGCCTGT CCCCTTCCCC
    CCGGACGCCC TTATCGGCCC CGGCATTCCT CGACACGCTC GCCAGATCAA CACCCTAAAC
    CATGGGGAGG TGGTGTGTGC GGTGACCATC AGCAACCCCA CGCGACACGT GTACACGGGT
    GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAACAAGAG CCCCGTCTCT
    CAGCTCGACT GTCTGAACAG GGATAACTAC ATCCGTTCCT GCCGATTGCT CCCTGATGGT
    CGTACCCTAA TTGTGGGAGG GGAAGCCAGT ACGCTGTCCA TCTGGGACCT GGCAGCTCCC
    ACCCCGCGCA TCAAGGCAGA GCTGACGTCC TCAGCCCCCG CCTGCTACGC CCTGGCCATC
    AGCCCGGACT CCAAGGTCTG CTTCTCGTGC TGCAGTGACG GCAACATTGC TGTGTGGGAC
    CTGCACAACC AGACGCTGGT GAGGCAATTC CAGGGCCACA CGGACGGGGC CAGCTGTATT
    GACATCTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGCT TGGACAACAC GGTCCGGTCC
    TGGGACCTTC GTGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTTTCT
    CTGGGCTACT GCCCGACCGG AGAGTGGCTG GCTGTGGGGA TGGAAAACAG CAACGTGGAG
    GTGTTGCATG TCACCAAGCC CGACAAGTAC CAGCTCCACC TGCACGAGAG CTGCGTGCTG
    TCGCTCAAGT TTGCCCACTG TGGCAAATGG TTTGTCAGCA CGGGAAAGGA CAACCTTCTG
    AATGCCTGGA GAACACCTTA TGGGGCCAGT ATATTCCAGT CCAAAGAATC CTCATCGGTG
    CTCAGCTGTG ACATTTCTGT GGATGACAAA TACATTGTCA CTGGCTCTGG GGATAAGAAG
    GCCACAGTTT ATGAAGTTAT TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa236999
    Clone ID Related Accession (Same CDS sequence) XM_024995564.1
    Accession Version XM_024995564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2187bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transducin-like enhancer protein 4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCAGCAGCA ACTCCAGGCC CAGCATCTAT CACATGGACA TGGTCTGCCA
    GTGCCTCTGA CCCCACACCC TTCGGGGCTT CAGCCTCCTG CCATTCCGCC CATTGGTAGC
    AGTGCCGGCC TTCTGGCCCT CTCCAGTGCC CTGGGAGGCC AGTCCCACCT TCCAATTAAA
    GATGAGAAGA AGCATCATGA CAGTGATCAC CAAAGAGACA GAGACTCCAT CAAGAGCTCT
    TCGGTATCCC CATCAGCCAG TTTCCGAGGT GCTGAGAAGC ACAGAAACTC CACAGACTAT
    TCCTCTGATA GCAAAAAGCA GAAAACTGAA GAAAAGGAGA TTGCAGCTCG TTATGACAGC
    GATGCTGAGA AGAGTGATGA CAACTTGGTG GTTGACGTTT CCAATGAGGA TCCATCATCA
    CCTCGAGGGA GCCCAGCACA TTCCCCGAGA GAGAACGGCC TGGACAAGAC ACGCCTGCTC
    AAGAAAGATG CCCCAATCAG TCCAGCTTCG ATTGCATCCT CCAGCAGTAC TCCTTCCTCC
    AAATCCAAAG AACTTAGCCT TAACGAAAAA TCTACTACTC CTGTGTCGAA GTCCAATACC
    CCTACTCCAC GAACTGATGC ACCCACCCCA GGCAGTAATT CCACTCCCGG ATTGAGGCCG
    GTTCCCGGAA AACCGCCAGG AGTCGACCCG TTGGCTTCAA GCCTAAGGAC TCCCATGGCA
    GTACCTTGTC CATACCCAAC CCCGTTTGGG ATTGTGCCAC ACGCGGGGAT GAATGGGGAG
    CTGACCAGCC CCGGAGCTGC CTACGCAGGG CTACATAACA TCTCCCCACA GATGAGCGCG
    GCTGCTGCTG CTGCCGCCGC GGCTGCTGCC TACGGGAGAT CACCAGTGGT TGGATTTGAT
    CCACACCATC ACATGCGTGT GCCAGCGATA CCTCCAAACC TGACAGGCAT TCCAGGAGGA
    AAGCCGGCAT ACTCCTTCCA CGTTACCGCA GATGGTCAGA TGCAGCCTGT CCCCTTCCCC
    CCGGACGCCC TTATCGGCCC CGGCATTCCT CGACACGCTC GCCAGATCAA CACCCTAAAC
    CATGGGGAGG TGGTGTGTGC GGTGACCATC AGCAACCCCA CGCGACACGT GTACACGGGT
    GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAACAAGAG CCCCGTCTCT
    CAGCTCGACT GTCTGAACAG GGATAACTAC ATCCGTTCCT GCCGATTGCT CCCTGATGGT
    CGTACCCTAA TTGTGGGAGG GGAAGCCAGT ACGCTGTCCA TCTGGGACCT GGCAGCTCCC
    ACCCCGCGCA TCAAGGCAGA GCTGACGTCC TCAGCCCCCG CCTGCTACGC CCTGGCCATC
    AGCCCGGACT CCAAGGTCTG CTTCTCGTGC TGCAGTGACG GCAACATTGC TGTGTGGGAC
    CTGCACAACC AGACGCTGGT GAGGCAATTC CAGGGCCACA CGGACGGGGC CAGCTGTATT
    GACATCTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGCT TGGACAACAC GGTCCGGTCC
    TGGGACCTTC GTGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTTTCT
    CTGGGCTACT GCCCGACCGG AGAGTGGCTG GCTGTGGGGA TGGAAAACAG CAACGTGGAG
    GTGTTGCATG TCACCAAGCC CGACAAGTAC CAGCTCCACC TGCACGAGAG CTGCGTGCTG
    TCGCTCAAGT TTGCCCACTG TGGCAAATGG TTTGTCAGCA CGGGAAAGGA CAACCTTCTG
    AATGCCTGGA GAACACCTTA TGGGGCCAGT ATATTCCAGT CCAAAGAATC CTCATCGGTG
    CTCAGCTGTG ACATTTCTGT GGATGACAAA TACATTGTCA CTGGCTCTGG GGATAAGAAG
    GCCACAGTTT ATGAAGTTAT TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024851332.1
    CDS882..3068
    Translation

    Target ORF information:

    RefSeq Version XM_024995564.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transducin like enhancer of split 4 (TLE4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024995564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGATTCGCG ACCTGAGCAA GATGTACCCG CAGACCAGAC ACCCGGCACC GCATCAGCCT 
    GCTCAACCCT TTAAATTTAC AATTTCCGAG TCCTGTGATC GGATTAAGGA AGAGTTTCAG
    TTTTTACAGG CTCAGTACCA CAGCCTGAAA CTGGAATGTG AGAAACTCGC CAGTGAGAAG
    ACAGAGATGC AGCGGCATTA TGTGATGTAT TATGAGATGT CCTATGGGTT GAATATAGAA
    ATGCACAAGC AGCAGCAGCA ACTCCAGGCC CAGCATCTAT CACATGGACA TGGTCTGCCA
    GTGCCTCTGA CCCCACACCC TTCGGGGCTT CAGCCTCCTG CCATTCCGCC CATTGGTAGC
    AGTGCCGGCC TTCTGGCCCT CTCCAGTGCC CTGGGAGGCC AGTCCCACCT TCCAATTAAA
    GATGAGAAGA AGCATCATGA CAGTGATCAC CAAAGAGACA GAGACTCCAT CAAGAGCTCT
    TCGGTATCCC CATCAGCCAG TTTCCGAGGT GCTGAGAAGC ACAGAAACTC CACAGACTAT
    TCCTCTGATA GCAAAAAGCA GAAAACTGAA GAAAAGGAGA TTGCAGCTCG TTATGACAGC
    GATGCTGAGA AGAGTGATGA CAACTTGGTG GTTGACGTTT CCAATGAGGA TCCATCATCA
    CCTCGAGGGA GCCCAGCACA TTCCCCGAGA GAGAACGGCC TGGACAAGAC ACGCCTGCTC
    AAGAAAGATG CCCCAATCAG TCCAGCTTCG ATTGCATCCT CCAGCAGTAC TCCTTCCTCC
    AAATCCAAAG AACTTAGCCT TAACGAAAAA TCTACTACTC CTGTGTCGAA GTCCAATACC
    CCTACTCCAC GAACTGATGC ACCCACCCCA GGCAGTAATT CCACTCCCGG ATTGAGGCCG
    GTTCCCGGAA AACCGCCAGG AGTCGACCCG TTGGCTTCAA GCCTAAGGAC TCCCATGGCA
    GTACCTTGTC CATACCCAAC CCCGTTTGGG ATTGTGCCAC ACGCGGGGAT GAATGGGGAG
    CTGACCAGCC CCGGAGCTGC CTACGCAGGG CTACATAACA TCTCCCCACA GATGAGCGCG
    GCTGCTGCTG CTGCCGCCGC GGCTGCTGCC TACGGGAGAT CACCAGTGGT TGGATTTGAT
    CCACACCATC ACATGCGTGT GCCAGCGATA CCTCCAAACC TGACAGGCAT TCCAGGAGGA
    AAGCCGGCAT ACTCCTTCCA CGTTACCGCA GATGGTCAGA TGCAGCCTGT CCCCTTCCCC
    CCGGACGCCC TTATCGGCCC CGGCATTCCT CGACACGCTC GCCAGATCAA CACCCTAAAC
    CATGGGGAGG TGGTGTGTGC GGTGACCATC AGCAACCCCA CGCGACACGT GTACACGGGT
    GGGAAGGGCT GCGTCAAGGT CTGGGACATC AGCCACCCTG GCAACAAGAG CCCCGTCTCT
    CAGCTCGACT GTCTGAACAG GGATAACTAC ATCCGTTCCT GCCGATTGCT CCCTGATGGT
    CGTACCCTAA TTGTGGGAGG GGAAGCCAGT ACGCTGTCCA TCTGGGACCT GGCAGCTCCC
    ACCCCGCGCA TCAAGGCAGA GCTGACGTCC TCAGCCCCCG CCTGCTACGC CCTGGCCATC
    AGCCCGGACT CCAAGGTCTG CTTCTCGTGC TGCAGTGACG GCAACATTGC TGTGTGGGAC
    CTGCACAACC AGACGCTGGT GAGGCAATTC CAGGGCCACA CGGACGGGGC CAGCTGTATT
    GACATCTCTA ATGATGGCAC CAAGCTCTGG ACGGGTGGCT TGGACAACAC GGTCCGGTCC
    TGGGACCTTC GTGAGGGGCG GCAGCTGCAG CAGCACGACT TCACCTCCCA GATCTTTTCT
    CTGGGCTACT GCCCGACCGG AGAGTGGCTG GCTGTGGGGA TGGAAAACAG CAACGTGGAG
    GTGTTGCATG TCACCAAGCC CGACAAGTAC CAGCTCCACC TGCACGAGAG CTGCGTGCTG
    TCGCTCAAGT TTGCCCACTG TGGCAAATGG TTTGTCAGCA CGGGAAAGGA CAACCTTCTG
    AATGCCTGGA GAACACCTTA TGGGGCCAGT ATATTCCAGT CCAAAGAATC CTCATCGGTG
    CTCAGCTGTG ACATTTCTGT GGATGACAAA TACATTGTCA CTGGCTCTGG GGATAAGAAG
    GCCACAGTTT ATGAAGTTAT TTATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.