ATP8A2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	ATP8A2
Entrez Gene ID	508723
Full Name	ATPase, aminophospholipid transporter, class I, type 8A, member 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024999818.1	XP_024855586.1	phospholipid-transporting ATPase IB isoform X1
XM_024999819.1	XP_024855587.1	phospholipid-transporting ATPase IB isoform X2
NM_001163802.3	NP_001157274.3	phospholipid-transporting ATPase IB
XM_024999820.1	XP_024855588.1	phospholipid-transporting ATPase IB isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	ATP8A2	XP_003433345.1
Bos taurus (cattle)	ATP8A2	NP_001157274.3
Gallus gallus (chicken)	ATP8A2	XP_417130.4
Saccharomyces cerevisiae (baker's yeast)	DRS2	NP_009376.2
Neurospora crassa	NCU00352	XP_957823.1
Homo sapiens (human)	ATP8A2	NP_057613.4
Mus musculus (house mouse)	Atp8a2	NP_056618.1
Danio rerio (zebrafish)	atp8a2	XP_005162794.1
Kluyveromyces lactis	KLLA0A04015g	XP_451177.1
Magnaporthe oryzae (rice blast fungus)	MGG_12922	XP_003712724.1
Oryza sativa (rice)	Os10g0412000	NP_001064586.1
Pan troglodytes (chimpanzee)	ATP8A2	XP_522636.3
Schizosaccharomyces pombe (fission yeast)	SPBC887.12	NP_596486.1
Arabidopsis thaliana (thale cress)	ALA3	NP_176191.1
	ATP8A2	XP_001092901.2
Rattus norvegicus (Norway rat)	LOC691889	XP_003751540.1
Eremothecium gossypii	AGOS_ADR350W	NP_984446.2
Xenopus tropicalis (tropical clawed frog)	atp8a2	XP_004912100.1

Gene Ontology
Bibliography

Related articles in PubMed

Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.
Vestergaard AL, Mikkelsen SA, Coleman JA, Molday RS, Vilsen B, Andersen JP
FEBS letters589(24 Pt B)3908-14(2015 Dec)

Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.
Coleman JA, Molday RS
The Journal of biological chemistry286(19)17205-16(2011 May)

Localization, purification, and functional reconstitution of the P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor disc membranes.
Coleman JA, Kwok MC, Molday RS
The Journal of biological chemistry284(47)32670-9(2009 Nov)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit
Title: Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.

CDC50A is the beta-subunit of ATP8A2 and is crucial for the correct folding, stable expression, export from endoplasmic reticulum, and phosphatidylserine flippase activity of ATP8A2
Title: Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.

P4-ATPase Atp8a2 is a phosphatidylserine flippase in photoreceptor disc membranes
Title: Localization, purification, and functional reconstitution of the P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor disc membranes.

The following ATP8A2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ATP8A2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa221115	XM_024999818.1 Latest version!	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa221116	XM_024999819.1 Latest version!	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa221117	XM_024999820.1 Latest version!	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa02292	NM_001163802.3 Latest version!	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ATP8A2 ( XM_024999818.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855586.1 Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa221115
Clone ID Related Accession (Same CDS sequence)	XM_024999818.1
Accession Version	XM_024999818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3567bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phospholipid-transporting ATPase IB isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAACG GCCCCGGCCG CGCCCAGGCT CTTAGGATGT CCCTGCCAGG GAGGCCACGG 
ATCCGCGCGT CCGTGGGACC TGTCCGTCCT CCCCCGGGCT ACAAGAAGGC AGACGATGAG 
ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGACAC AGATATGTAT 
TATCTAGGAA ATGACACCCC TGCCATGGCC AGGACGTCAA ACCTTAATGA AGAGCTTGGC 
CAGGTAAAAT ATCTATTTTC TGACAAAACA GGAACTCTGA CATGCAATAT CATGAACTTC 
AAAAAGTGTA GCATTGCTGG GGTAACCTAT GGTCATTTTC CAGAACTGAC AAGGGAACCA 
TCCTCAGATG ATTTCTCCCG GATTCCTCCT CCACCTAGTG ATTCCTGCGA CTTTGATGAC 
CCCAGGCTCT TGAAGAACAT CGAGGATCAT CATCCCACAG CACCCTGCAT CCAGGAGTTC 
CTGACCCTGC TGGCCGTGTG CCACACCGTG GTCCCCGAGA GGGACGGGGA CAGCATCGTC 
TACCAGGCCT CCTCCCCAGA CGAAGCCGCT TTGGTCAAAG GGGCCCGGAA GCTGGGCTTT 
GTCTTCACAG CCAGAACGCC CTACTCTGTC ATCATCGAGG CCATGGGACA GGAACAGACA 
TTTGGAATCC TGAATGTCCT GGAATTCTCC AGCGACAGAA AGAGAATGTC TGTGATAGTG 
CGAACTCCTT CGGGACAGCT TCGACTCTAC TGTAAAGGGG CTGATAACGT GATTTTTGAG 
AGACTTTCAA AAGATTCAAA ATACATGGAG GAGACTCTCT GCCACCTGGA GTACTTTGCC 
ACGGAAGGCT TGCGGACTCT GTGTGTGGCT TACGCCGACC TCAGTGAGCG GGATTACGAG 
GAGTGGCTCA AAGTCTACCA GGAAGCCAGC ACCATCCTGA AGGACCGCGC TCAGCGGCTG 
GAGGAGTGCT ACGAGATCAT CGAGAAGAAT CTACTGCTAC TTGGAGCCAC GGCCATAGAA 
GATCGACTTC AGGCAGGCGT GCCGGAAACC ATAGCAACTC TGTTGAAGGC CGAGATTAAA 
ATATGGGTGT TGACAGGCGA CAAACAAGAA ACTGCTATTA ATATAGGTTA TTCCTGTCGG 
TTGGTGTCAC AGAACATGGC CCTTATCCTA CTGAAGGAGG ATTCTTTGGA CGCCACCCGG 
GCAGCCATCA CCCAGCACTG CGCGGACCTC GGGAGCCTGC TGGGCAAGGA GAACGACGCG 
GCCCTCATCA TCGACGGCCA CACCCTCAAG TACGCGCTGT CCTTCGAGGT CCGGAGGAGC 
TTCCTGGACC TGGCCCTCTC GTGCAAGGCA GTCATCTGTT GCAGGGTGTC TCCTCTGCAG 
AAGTCGGAGA TAGTGGATGT GGTGAAGAAG CGGGTGAAAG CCATCACCCT GGCCATTGGG 
GATGGTGCCA ACGATGTGGG CATGATCCAG ACGGCACACG TGGGTGTAGG CATCAGTGGG 
AACGAGGGCA TGCAGGCCAC TAACAACTCG GATTACGCCA TCGCTCAGTT TTCCTACTTG 
GAGAAGCTCC TGCTGGTTCA CGGCGCCTGG AGCTACAACC GGGTGACCAA GTGCATCTTG 
TACTGTTTCT ACAAGAACGT GGTGCTCTAC ATCATTGAGC TTTGGTTTGC CTTCGTTAAT 
GGATTTTCTG GGCAGATTTT ATTTGAACGT TGGTGCATCG GCTTGTACAA CGTGATTTTC 
ACGGCACTGC CCCCCTTCAC GCTGGGCATC TTCGAGAGGT CTTGCAGCCA GGAGAGCATG 
CTGAGGTTCC CGCAGCTCTA CAAGATCACC CAGAACGCCG AGGGCTTCAA CACCAAGGTT 
TTCTGGGGTC ACTGCATCAA CGCCTTGGTC CACTCACTCA TCCTGTTCTG GTTTCCCATG 
AAAGCGCTGG AGCATGATAC TGTGTTGGCC AATGGTCATG CCACAGACTA TTTATTTGTT 
GGAAATATCG TTTATACTTA TGTTGTTGTT ACTGTTTGTC TGAAAGCTGG TTTGGAGACG 
ACAGCTTGGA CCAAATTCAG TCACCTGGCC GTCTGGGGAA GCATGCTCAT CTGGCTGGTG 
TTCTTTGGCA TCTACTCGAC CATCTGGCCC ACCATTCCCA TCGCTCCAGA CATGAAAGGG 
CAGGCAACCA TGGTTCTGAG CTCTGCACAC TTCTGGCTGG GACTATTTCT GGTTCCCACC 
GCATGTCTGA TCGAAGATGT GGCATGGAGA GCGGCCAAGC ACACCTGCAA GAAAACGCTT 
CTGGAGGAAG TGCAGGAGCT GGAGATGAAG TCCAGAGTGA TGGGCAGAGC AATGCTCCGG 
GACAGTAACG GAAAGAGGAT GAACGAGCGA GACCGGCTGC TAAAGAGGCT GAGCAGGAAG 
ACGCCACCCA CCCTCTTCCG GGGCAGCTCC CTCCAGCAGT CCATGCCACA TGGGTATGCC 
TTCTCCCAGG AGGAACACGG AGCCGTGACT CAGGAGGAAA TCGTCCGCGC TTACGACACC 
ACGAAGCAGA AATCCAGGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855586.1
CDS	20..3586
Translation	MPNGPGRAQALRMSLPGRPRIRASVGPVRPPPGYKKADDEMSRATSVGDQLDVPARTIYLNQPHLNKFCDNQISTAKYSVVTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDFKRHKADNAVNKKKTIVLRNGMWQTIVWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGETNLKIRQGLSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSPVALGPDQILLRGTQLRNTQWGFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALYWNGSQGGKNWYIKKMDATSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALFINWDTDMYYLGNDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELTREPSSDDFSRIPPPPSDSCDFDDPRLLKNIEDHHPTAPCIQEFLTLLAVCHTVVPERDGDSIVYQASSPDEAALVKGARKLGFVFTARTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRTPSGQLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSERDYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCADLGSLLGKENDAALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCSQESMLRFPQLYKITQNAEGFNTKVFWGHCINALVHSLILFWFPMKALEHDTVLANGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLIWLVFFGIYSTIWPTIPIAPDMKGQATMVLSSAHFWLGLFLVPTACLIEDVAWRAAKHTCKKTLLEEVQELEMKSRVMGRAMLRDSNGKRMNERDRLLKRLSRKTPPTLFRGSSLQQSMPHGYAFSQEEHGAVTQEEIVRAYDTTKQKSRKK

Target ORF information:

RefSeq Version	XM_024999818.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999818.1

ORF Insert Sequence:

ATGCCCAACG GCCCCGGCCG CGCCCAGGCT CTTAGGATGT CCCTGCCAGG GAGGCCACGG 
ATCCGCGCGT CCGTGGGACC TGTCCGTCCT CCCCCGGGCT ACAAGAAGGC AGACGATGAG 
ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGACAC AGATATGTAT 
TATCTAGGAA ATGACACCCC TGCCATGGCC AGGACGTCAA ACCTTAATGA AGAGCTTGGC 
CAGGTAAAAT ATCTATTTTC TGACAAAACA GGAACTCTGA CATGCAATAT CATGAACTTC 
AAAAAGTGTA GCATTGCTGG GGTAACCTAT GGTCATTTTC CAGAACTGAC AAGGGAACCA 
TCCTCAGATG ATTTCTCCCG GATTCCTCCT CCACCTAGTG ATTCCTGCGA CTTTGATGAC 
CCCAGGCTCT TGAAGAACAT CGAGGATCAT CATCCCACAG CACCCTGCAT CCAGGAGTTC 
CTGACCCTGC TGGCCGTGTG CCACACCGTG GTCCCCGAGA GGGACGGGGA CAGCATCGTC 
TACCAGGCCT CCTCCCCAGA CGAAGCCGCT TTGGTCAAAG GGGCCCGGAA GCTGGGCTTT 
GTCTTCACAG CCAGAACGCC CTACTCTGTC ATCATCGAGG CCATGGGACA GGAACAGACA 
TTTGGAATCC TGAATGTCCT GGAATTCTCC AGCGACAGAA AGAGAATGTC TGTGATAGTG 
CGAACTCCTT CGGGACAGCT TCGACTCTAC TGTAAAGGGG CTGATAACGT GATTTTTGAG 
AGACTTTCAA AAGATTCAAA ATACATGGAG GAGACTCTCT GCCACCTGGA GTACTTTGCC 
ACGGAAGGCT TGCGGACTCT GTGTGTGGCT TACGCCGACC TCAGTGAGCG GGATTACGAG 
GAGTGGCTCA AAGTCTACCA GGAAGCCAGC ACCATCCTGA AGGACCGCGC TCAGCGGCTG 
GAGGAGTGCT ACGAGATCAT CGAGAAGAAT CTACTGCTAC TTGGAGCCAC GGCCATAGAA 
GATCGACTTC AGGCAGGCGT GCCGGAAACC ATAGCAACTC TGTTGAAGGC CGAGATTAAA 
ATATGGGTGT TGACAGGCGA CAAACAAGAA ACTGCTATTA ATATAGGTTA TTCCTGTCGG 
TTGGTGTCAC AGAACATGGC CCTTATCCTA CTGAAGGAGG ATTCTTTGGA CGCCACCCGG 
GCAGCCATCA CCCAGCACTG CGCGGACCTC GGGAGCCTGC TGGGCAAGGA GAACGACGCG 
GCCCTCATCA TCGACGGCCA CACCCTCAAG TACGCGCTGT CCTTCGAGGT CCGGAGGAGC 
TTCCTGGACC TGGCCCTCTC GTGCAAGGCA GTCATCTGTT GCAGGGTGTC TCCTCTGCAG 
AAGTCGGAGA TAGTGGATGT GGTGAAGAAG CGGGTGAAAG CCATCACCCT GGCCATTGGG 
GATGGTGCCA ACGATGTGGG CATGATCCAG ACGGCACACG TGGGTGTAGG CATCAGTGGG 
AACGAGGGCA TGCAGGCCAC TAACAACTCG GATTACGCCA TCGCTCAGTT TTCCTACTTG 
GAGAAGCTCC TGCTGGTTCA CGGCGCCTGG AGCTACAACC GGGTGACCAA GTGCATCTTG 
TACTGTTTCT ACAAGAACGT GGTGCTCTAC ATCATTGAGC TTTGGTTTGC CTTCGTTAAT 
GGATTTTCTG GGCAGATTTT ATTTGAACGT TGGTGCATCG GCTTGTACAA CGTGATTTTC 
ACGGCACTGC CCCCCTTCAC GCTGGGCATC TTCGAGAGGT CTTGCAGCCA GGAGAGCATG 
CTGAGGTTCC CGCAGCTCTA CAAGATCACC CAGAACGCCG AGGGCTTCAA CACCAAGGTT 
TTCTGGGGTC ACTGCATCAA CGCCTTGGTC CACTCACTCA TCCTGTTCTG GTTTCCCATG 
AAAGCGCTGG AGCATGATAC TGTGTTGGCC AATGGTCATG CCACAGACTA TTTATTTGTT 
GGAAATATCG TTTATACTTA TGTTGTTGTT ACTGTTTGTC TGAAAGCTGG TTTGGAGACG 
ACAGCTTGGA CCAAATTCAG TCACCTGGCC GTCTGGGGAA GCATGCTCAT CTGGCTGGTG 
TTCTTTGGCA TCTACTCGAC CATCTGGCCC ACCATTCCCA TCGCTCCAGA CATGAAAGGG 
CAGGCAACCA TGGTTCTGAG CTCTGCACAC TTCTGGCTGG GACTATTTCT GGTTCCCACC 
GCATGTCTGA TCGAAGATGT GGCATGGAGA GCGGCCAAGC ACACCTGCAA GAAAACGCTT 
CTGGAGGAAG TGCAGGAGCT GGAGATGAAG TCCAGAGTGA TGGGCAGAGC AATGCTCCGG 
GACAGTAACG GAAAGAGGAT GAACGAGCGA GACCGGCTGC TAAAGAGGCT GAGCAGGAAG 
ACGCCACCCA CCCTCTTCCG GGGCAGCTCC CTCCAGCAGT CCATGCCACA TGGGTATGCC 
TTCTCCCAGG AGGAACACGG AGCCGTGACT CAGGAGGAAA TCGTCCGCGC TTACGACACC 
ACGAAGCAGA AATCCAGGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8A2 ( XM_024999819.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855587.1 Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa221116
Clone ID Related Accession (Same CDS sequence)	XM_024999819.1
Accession Version	XM_024999819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phospholipid-transporting ATPase IB isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCAACG GCCCCGGCCG CGCCCAGGCT CTTAGGATGT CCCTGCCAGG GAGGCCACGG 
ATCCGCGCGT CCGTGGGACC TGTCCGTCCT CCCCCGGGCT ACAAGAAGGC AGACGATGAG 
ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGTAAA ATATCTATTT 
TCTGACAAAA CAGGAACTCT GACATGCAAT ATCATGAACT TCAAAAAGTG TAGCATTGCT 
GGGGTAACCT ATGGTCATTT TCCAGAACTG ACAAGGGAAC CATCCTCAGA TGATTTCTCC 
CGGATTCCTC CTCCACCTAG TGATTCCTGC GACTTTGATG ACCCCAGGCT CTTGAAGAAC 
ATCGAGGATC ATCATCCCAC AGCACCCTGC ATCCAGGAGT TCCTGACCCT GCTGGCCGTG 
TGCCACACCG TGGTCCCCGA GAGGGACGGG GACAGCATCG TCTACCAGGC CTCCTCCCCA 
GACGAAGCCG CTTTGGTCAA AGGGGCCCGG AAGCTGGGCT TTGTCTTCAC AGCCAGAACG 
CCCTACTCTG TCATCATCGA GGCCATGGGA CAGGAACAGA CATTTGGAAT CCTGAATGTC 
CTGGAATTCT CCAGCGACAG AAAGAGAATG TCTGTGATAG TGCGAACTCC TTCGGGACAG 
CTTCGACTCT ACTGTAAAGG GGCTGATAAC GTGATTTTTG AGAGACTTTC AAAAGATTCA 
AAATACATGG AGGAGACTCT CTGCCACCTG GAGTACTTTG CCACGGAAGG CTTGCGGACT 
CTGTGTGTGG CTTACGCCGA CCTCAGTGAG CGGGATTACG AGGAGTGGCT CAAAGTCTAC 
CAGGAAGCCA GCACCATCCT GAAGGACCGC GCTCAGCGGC TGGAGGAGTG CTACGAGATC 
ATCGAGAAGA ATCTACTGCT ACTTGGAGCC ACGGCCATAG AAGATCGACT TCAGGCAGGC 
GTGCCGGAAA CCATAGCAAC TCTGTTGAAG GCCGAGATTA AAATATGGGT GTTGACAGGC 
GACAAACAAG AAACTGCTAT TAATATAGGT TATTCCTGTC GGTTGGTGTC ACAGAACATG 
GCCCTTATCC TACTGAAGGA GGATTCTTTG GACGCCACCC GGGCAGCCAT CACCCAGCAC 
TGCGCGGACC TCGGGAGCCT GCTGGGCAAG GAGAACGACG CGGCCCTCAT CATCGACGGC 
CACACCCTCA AGTACGCGCT GTCCTTCGAG GTCCGGAGGA GCTTCCTGGA CCTGGCCCTC 
TCGTGCAAGG CAGTCATCTG TTGCAGGGTG TCTCCTCTGC AGAAGTCGGA GATAGTGGAT 
GTGGTGAAGA AGCGGGTGAA AGCCATCACC CTGGCCATTG GGGATGGTGC CAACGATGTG 
GGCATGATCC AGACGGCACA CGTGGGTGTA GGCATCAGTG GGAACGAGGG CATGCAGGCC 
ACTAACAACT CGGATTACGC CATCGCTCAG TTTTCCTACT TGGAGAAGCT CCTGCTGGTT 
CACGGCGCCT GGAGCTACAA CCGGGTGACC AAGTGCATCT TGTACTGTTT CTACAAGAAC 
GTGGTGCTCT ACATCATTGA GCTTTGGTTT GCCTTCGTTA ATGGATTTTC TGGGCAGATT 
TTATTTGAAC GTTGGTGCAT CGGCTTGTAC AACGTGATTT TCACGGCACT GCCCCCCTTC 
ACGCTGGGCA TCTTCGAGAG GTCTTGCAGC CAGGAGAGCA TGCTGAGGTT CCCGCAGCTC 
TACAAGATCA CCCAGAACGC CGAGGGCTTC AACACCAAGG TTTTCTGGGG TCACTGCATC 
AACGCCTTGG TCCACTCACT CATCCTGTTC TGGTTTCCCA TGAAAGCGCT GGAGCATGAT 
ACTGTGTTGG CCAATGGTCA TGCCACAGAC TATTTATTTG TTGGAAATAT CGTTTATACT 
TATGTTGTTG TTACTGTTTG TCTGAAAGCT GGTTTGGAGA CGACAGCTTG GACCAAATTC 
AGTCACCTGG CCGTCTGGGG AAGCATGCTC ATCTGGCTGG TGTTCTTTGG CATCTACTCG 
ACCATCTGGC CCACCATTCC CATCGCTCCA GACATGAAAG GGCAGGCAAC CATGGTTCTG 
AGCTCTGCAC ACTTCTGGCT GGGACTATTT CTGGTTCCCA CCGCATGTCT GATCGAAGAT 
GTGGCATGGA GAGCGGCCAA GCACACCTGC AAGAAAACGC TTCTGGAGGA AGTGCAGGAG 
CTGGAGATGA AGTCCAGAGT GATGGGCAGA GCAATGCTCC GGGACAGTAA CGGAAAGAGG 
ATGAACGAGC GAGACCGGCT GCTAAAGAGG CTGAGCAGGA AGACGCCACC CACCCTCTTC 
CGGGGCAGCT CCCTCCAGCA GTCCATGCCA CATGGGTATG CCTTCTCCCA GGAGGAACAC 
GGAGCCGTGA CTCAGGAGGA AATCGTCCGC GCTTACGACA CCACGAAGCA GAAATCCAGG 
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855587.1
CDS	20..3508
Translation	MPNGPGRAQALRMSLPGRPRIRASVGPVRPPPGYKKADDEMSRATSVGDQLDVPARTIYLNQPHLNKFCDNQISTAKYSVVTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDFKRHKADNAVNKKKTIVLRNGMWQTIVWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGETNLKIRQGLSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSPVALGPDQILLRGTQLRNTQWGFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALYWNGSQGGKNWYIKKMDATSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALFINWVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELTREPSSDDFSRIPPPPSDSCDFDDPRLLKNIEDHHPTAPCIQEFLTLLAVCHTVVPERDGDSIVYQASSPDEAALVKGARKLGFVFTARTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRTPSGQLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSERDYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCADLGSLLGKENDAALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCSQESMLRFPQLYKITQNAEGFNTKVFWGHCINALVHSLILFWFPMKALEHDTVLANGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLIWLVFFGIYSTIWPTIPIAPDMKGQATMVLSSAHFWLGLFLVPTACLIEDVAWRAAKHTCKKTLLEEVQELEMKSRVMGRAMLRDSNGKRMNERDRLLKRLSRKTPPTLFRGSSLQQSMPHGYAFSQEEHGAVTQEEIVRAYDTTKQKSRKK

Target ORF information:

RefSeq Version	XM_024999819.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999819.1

ORF Insert Sequence:

ATGCCCAACG GCCCCGGCCG CGCCCAGGCT CTTAGGATGT CCCTGCCAGG GAGGCCACGG 
ATCCGCGCGT CCGTGGGACC TGTCCGTCCT CCCCCGGGCT ACAAGAAGGC AGACGATGAG 
ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGTAAA ATATCTATTT 
TCTGACAAAA CAGGAACTCT GACATGCAAT ATCATGAACT TCAAAAAGTG TAGCATTGCT 
GGGGTAACCT ATGGTCATTT TCCAGAACTG ACAAGGGAAC CATCCTCAGA TGATTTCTCC 
CGGATTCCTC CTCCACCTAG TGATTCCTGC GACTTTGATG ACCCCAGGCT CTTGAAGAAC 
ATCGAGGATC ATCATCCCAC AGCACCCTGC ATCCAGGAGT TCCTGACCCT GCTGGCCGTG 
TGCCACACCG TGGTCCCCGA GAGGGACGGG GACAGCATCG TCTACCAGGC CTCCTCCCCA 
GACGAAGCCG CTTTGGTCAA AGGGGCCCGG AAGCTGGGCT TTGTCTTCAC AGCCAGAACG 
CCCTACTCTG TCATCATCGA GGCCATGGGA CAGGAACAGA CATTTGGAAT CCTGAATGTC 
CTGGAATTCT CCAGCGACAG AAAGAGAATG TCTGTGATAG TGCGAACTCC TTCGGGACAG 
CTTCGACTCT ACTGTAAAGG GGCTGATAAC GTGATTTTTG AGAGACTTTC AAAAGATTCA 
AAATACATGG AGGAGACTCT CTGCCACCTG GAGTACTTTG CCACGGAAGG CTTGCGGACT 
CTGTGTGTGG CTTACGCCGA CCTCAGTGAG CGGGATTACG AGGAGTGGCT CAAAGTCTAC 
CAGGAAGCCA GCACCATCCT GAAGGACCGC GCTCAGCGGC TGGAGGAGTG CTACGAGATC 
ATCGAGAAGA ATCTACTGCT ACTTGGAGCC ACGGCCATAG AAGATCGACT TCAGGCAGGC 
GTGCCGGAAA CCATAGCAAC TCTGTTGAAG GCCGAGATTA AAATATGGGT GTTGACAGGC 
GACAAACAAG AAACTGCTAT TAATATAGGT TATTCCTGTC GGTTGGTGTC ACAGAACATG 
GCCCTTATCC TACTGAAGGA GGATTCTTTG GACGCCACCC GGGCAGCCAT CACCCAGCAC 
TGCGCGGACC TCGGGAGCCT GCTGGGCAAG GAGAACGACG CGGCCCTCAT CATCGACGGC 
CACACCCTCA AGTACGCGCT GTCCTTCGAG GTCCGGAGGA GCTTCCTGGA CCTGGCCCTC 
TCGTGCAAGG CAGTCATCTG TTGCAGGGTG TCTCCTCTGC AGAAGTCGGA GATAGTGGAT 
GTGGTGAAGA AGCGGGTGAA AGCCATCACC CTGGCCATTG GGGATGGTGC CAACGATGTG 
GGCATGATCC AGACGGCACA CGTGGGTGTA GGCATCAGTG GGAACGAGGG CATGCAGGCC 
ACTAACAACT CGGATTACGC CATCGCTCAG TTTTCCTACT TGGAGAAGCT CCTGCTGGTT 
CACGGCGCCT GGAGCTACAA CCGGGTGACC AAGTGCATCT TGTACTGTTT CTACAAGAAC 
GTGGTGCTCT ACATCATTGA GCTTTGGTTT GCCTTCGTTA ATGGATTTTC TGGGCAGATT 
TTATTTGAAC GTTGGTGCAT CGGCTTGTAC AACGTGATTT TCACGGCACT GCCCCCCTTC 
ACGCTGGGCA TCTTCGAGAG GTCTTGCAGC CAGGAGAGCA TGCTGAGGTT CCCGCAGCTC 
TACAAGATCA CCCAGAACGC CGAGGGCTTC AACACCAAGG TTTTCTGGGG TCACTGCATC 
AACGCCTTGG TCCACTCACT CATCCTGTTC TGGTTTCCCA TGAAAGCGCT GGAGCATGAT 
ACTGTGTTGG CCAATGGTCA TGCCACAGAC TATTTATTTG TTGGAAATAT CGTTTATACT 
TATGTTGTTG TTACTGTTTG TCTGAAAGCT GGTTTGGAGA CGACAGCTTG GACCAAATTC 
AGTCACCTGG CCGTCTGGGG AAGCATGCTC ATCTGGCTGG TGTTCTTTGG CATCTACTCG 
ACCATCTGGC CCACCATTCC CATCGCTCCA GACATGAAAG GGCAGGCAAC CATGGTTCTG 
AGCTCTGCAC ACTTCTGGCT GGGACTATTT CTGGTTCCCA CCGCATGTCT GATCGAAGAT 
GTGGCATGGA GAGCGGCCAA GCACACCTGC AAGAAAACGC TTCTGGAGGA AGTGCAGGAG 
CTGGAGATGA AGTCCAGAGT GATGGGCAGA GCAATGCTCC GGGACAGTAA CGGAAAGAGG 
ATGAACGAGC GAGACCGGCT GCTAAAGAGG CTGAGCAGGA AGACGCCACC CACCCTCTTC 
CGGGGCAGCT CCCTCCAGCA GTCCATGCCA CATGGGTATG CCTTCTCCCA GGAGGAACAC 
GGAGCCGTGA CTCAGGAGGA AATCGTCCGC GCTTACGACA CCACGAAGCA GAAATCCAGG 
AAGAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8A2 ( XM_024999820.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024855588.1 Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X3, mRNA.

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CloneID	OBa221117
Clone ID Related Accession (Same CDS sequence)	XM_024999820.1
Accession Version	XM_024999820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phospholipid-transporting ATPase IB isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037339.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGACAC AGATATGTAT 
TATCTAGGAA ATGACACCCC TGCCATGGCC AGGACGTCAA ACCTTAATGA AGAGCTTGGC 
CAGGTAAAAT ATCTATTTTC TGACAAAACA GGAACTCTGA CATGCAATAT CATGAACTTC 
AAAAAGTGTA GCATTGCTGG GGTAACCTAT GGTCATTTTC CAGAACTGAC AAGGGAACCA 
TCCTCAGATG ATTTCTCCCG GATTCCTCCT CCACCTAGTG ATTCCTGCGA CTTTGATGAC 
CCCAGGCTCT TGAAGAACAT CGAGGATCAT CATCCCACAG CACCCTGCAT CCAGGAGTTC 
CTGACCCTGC TGGCCGTGTG CCACACCGTG GTCCCCGAGA GGGACGGGGA CAGCATCGTC 
TACCAGGCCT CCTCCCCAGA CGAAGCCGCT TTGGTCAAAG GGGCCCGGAA GCTGGGCTTT 
GTCTTCACAG CCAGAACGCC CTACTCTGTC ATCATCGAGG CCATGGGACA GGAACAGACA 
TTTGGAATCC TGAATGTCCT GGAATTCTCC AGCGACAGAA AGAGAATGTC TGTGATAGTG 
CGAACTCCTT CGGGACAGCT TCGACTCTAC TGTAAAGGGG CTGATAACGT GATTTTTGAG 
AGACTTTCAA AAGATTCAAA ATACATGGAG GAGACTCTCT GCCACCTGGA GTACTTTGCC 
ACGGAAGGCT TGCGGACTCT GTGTGTGGCT TACGCCGACC TCAGTGAGCG GGATTACGAG 
GAGTGGCTCA AAGTCTACCA GGAAGCCAGC ACCATCCTGA AGGACCGCGC TCAGCGGCTG 
GAGGAGTGCT ACGAGATCAT CGAGAAGAAT CTACTGCTAC TTGGAGCCAC GGCCATAGAA 
GATCGACTTC AGGCAGGCGT GCCGGAAACC ATAGCAACTC TGTTGAAGGC CGAGATTAAA 
ATATGGGTGT TGACAGGCGA CAAACAAGAA ACTGCTATTA ATATAGGTTA TTCCTGTCGG 
TTGGTGTCAC AGAACATGGC CCTTATCCTA CTGAAGGAGG ATTCTTTGGA CGCCACCCGG 
GCAGCCATCA CCCAGCACTG CGCGGACCTC GGGAGCCTGC TGGGCAAGGA GAACGACGCG 
GCCCTCATCA TCGACGGCCA CACCCTCAAG TACGCGCTGT CCTTCGAGGT CCGGAGGAGC 
TTCCTGGACC TGGCCCTCTC GTGCAAGGCA GTCATCTGTT GCAGGGTGTC TCCTCTGCAG 
AAGTCGGAGA TAGTGGATGT GGTGAAGAAG CGGGTGAAAG CCATCACCCT GGCCATTGGG 
GATGGTGCCA ACGATGTGGG CATGATCCAG ACGGCACACG TGGGTGTAGG CATCAGTGGG 
AACGAGGGCA TGCAGGCCAC TAACAACTCG GATTACGCCA TCGCTCAGTT TTCCTACTTG 
GAGAAGCTCC TGCTGGTTCA CGGCGCCTGG AGCTACAACC GGGTGACCAA GTGCATCTTG 
TACTGTTTCT ACAAGAACGT GGTGCTCTAC ATCATTGAGC TTTGGTTTGC CTTCGTTAAT 
GGATTTTCTG GGCAGATTTT ATTTGAACGT TGGTGCATCG GCTTGTACAA CGTGATTTTC 
ACGGCACTGC CCCCCTTCAC GCTGGGCATC TTCGAGAGGT CTTGCAGCCA GGAGAGCATG 
CTGAGGTTCC CGCAGCTCTA CAAGATCACC CAGAACGCCG AGGGCTTCAA CACCAAGGTT 
TTCTGGGGTC ACTGCATCAA CGCCTTGGTC CACTCACTCA TCCTGTTCTG GTTTCCCATG 
AAAGCGCTGG AGCATGATAC TGTGTTGGCC AATGGTCATG CCACAGACTA TTTATTTGTT 
GGAAATATCG TTTATACTTA TGTTGTTGTT ACTGTTTGTC TGAAAGCTGG TTTGGAGACG 
ACAGCTTGGA CCAAATTCAG TCACCTGGCC GTCTGGGGAA GCATGCTCAT CTGGCTGGTG 
TTCTTTGGCA TCTACTCGAC CATCTGGCCC ACCATTCCCA TCGCTCCAGA CATGAAAGGG 
CAGGCAACCA TGGTTCTGAG CTCTGCACAC TTCTGGCTGG GACTATTTCT GGTTCCCACC 
GCATGTCTGA TCGAAGATGT GGCATGGAGA GCGGCCAAGC ACACCTGCAA GAAAACGCTT 
CTGGAGGAAG TGCAGGAGCT GGAGATGAAG TCCAGAGTGA TGGGCAGAGC AATGCTCCGG 
GACAGTAACG GAAAGAGGAT GAACGAGCGA GACCGGCTGC TAAAGAGGCT GAGCAGGAAG 
ACGCCACCCA CCCTCTTCCG GGGCAGCTCC CTCCAGCAGT CCATGCCACA TGGGTATGCC 
TTCTCCCAGG AGGAACACGG AGCCGTGACT CAGGAGGAAA TCGTCCGCGC TTACGACACC 
ACGAAGCAGA AATCCAGGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024855588.1
CDS	141..3587
Translation	MSRATSVGDQLDVPARTIYLNQPHLNKFCDNQISTAKYSVVTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDFKRHKADNAVNKKKTIVLRNGMWQTIVWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGETNLKIRQGLSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSPVALGPDQILLRGTQLRNTQWGFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALYWNGSQGGKNWYIKKMDATSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALFINWDTDMYYLGNDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELTREPSSDDFSRIPPPPSDSCDFDDPRLLKNIEDHHPTAPCIQEFLTLLAVCHTVVPERDGDSIVYQASSPDEAALVKGARKLGFVFTARTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRTPSGQLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSERDYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCADLGSLLGKENDAALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCSQESMLRFPQLYKITQNAEGFNTKVFWGHCINALVHSLILFWFPMKALEHDTVLANGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLIWLVFFGIYSTIWPTIPIAPDMKGQATMVLSSAHFWLGLFLVPTACLIEDVAWRAAKHTCKKTLLEEVQELEMKSRVMGRAMLRDSNGKRMNERDRLLKRLSRKTPPTLFRGSSLQQSMPHGYAFSQEEHGAVTQEEIVRAYDTTKQKSRKK

Target ORF information:

RefSeq Version	XM_024999820.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024999820.1

ORF Insert Sequence:

ATGTCCCGGG CCACGTCTGT TGGAGACCAG CTGGACGTAC CAGCCCGCAC CATTTACCTC 
AACCAACCGC ATCTCAACAA ATTCTGCGAC AACCAGATCA GCACGGCCAA GTACAGCGTG 
GTGACGTTTC TACCTCGATT CTTGTACGAG CAGATCAGGA GAGCTGCCAA TGCCTTCTTC 
CTCTTCATTG CCTTATTACA GCAAATTCCA GATGTATCTC CAACAGGAAG ATACACCACC 
CTGGTGCCAT TGATCATTAT TTTAACAATC GCAGGCATCA AAGAGATTGT AGAAGATTTT 
AAGCGACATA AGGCAGACAA TGCAGTTAAC AAAAAGAAAA CCATAGTGTT AAGGAATGGT 
ATGTGGCAAA CCATTGTGTG GAAAGAGGTG GCCGTGGGAG ACATCGTCAA AGTCGTCAAC 
GGGCAGTACC TTCCAGCAGA CGTGGTCCTG CTCTCCTCCA GTGAACCTCA GGCAATGTGT 
TACGTTGAAA CAGCCAATCT GGACGGGGAG ACGAACCTTA AAATACGTCA GGGTTTGAGT 
CACACTGCGG ACATGCAGAC GAGGGAGGTG CTGATGAAGT TGTCTGGGAC CATAGAGTGC 
GAAGGGCCCA ATCGCCACCT CTACGACTTC ACCGGGAACT TGAACTTGGA TGGGAAAAGC 
CCCGTTGCCC TTGGGCCGGA CCAGATCTTA TTGAGAGGCA CACAGCTTAG AAACACTCAG 
TGGGGCTTTG GCATAGTTGT CTACACTGGA CATGACACCA AGCTCATGCA GAATTCCACC 
AAAGCCCCTC TCAAGAGGTC GAATGTGGAG AAGGTAACCA ACGTGCAGAT CCTGGTACTG 
TTCGGCATCC TGCTGGTCAT GGCCCTGGTG AGCTCGGTGG GAGCCCTGTA CTGGAACGGC 
TCTCAAGGAG GGAAGAACTG GTACATCAAG AAGATGGATG CGACCTCAGA CAATTTCGGA 
TACAACCTGT TGACATTCAT CATCCTCTAC AACAATCTGA TTCCCATCAG TCTGCTGGTG 
ACTCTCGAGG TTGTGAAGTA CACACAAGCC CTCTTCATAA ACTGGGACAC AGATATGTAT 
TATCTAGGAA ATGACACCCC TGCCATGGCC AGGACGTCAA ACCTTAATGA AGAGCTTGGC 
CAGGTAAAAT ATCTATTTTC TGACAAAACA GGAACTCTGA CATGCAATAT CATGAACTTC 
AAAAAGTGTA GCATTGCTGG GGTAACCTAT GGTCATTTTC CAGAACTGAC AAGGGAACCA 
TCCTCAGATG ATTTCTCCCG GATTCCTCCT CCACCTAGTG ATTCCTGCGA CTTTGATGAC 
CCCAGGCTCT TGAAGAACAT CGAGGATCAT CATCCCACAG CACCCTGCAT CCAGGAGTTC 
CTGACCCTGC TGGCCGTGTG CCACACCGTG GTCCCCGAGA GGGACGGGGA CAGCATCGTC 
TACCAGGCCT CCTCCCCAGA CGAAGCCGCT TTGGTCAAAG GGGCCCGGAA GCTGGGCTTT 
GTCTTCACAG CCAGAACGCC CTACTCTGTC ATCATCGAGG CCATGGGACA GGAACAGACA 
TTTGGAATCC TGAATGTCCT GGAATTCTCC AGCGACAGAA AGAGAATGTC TGTGATAGTG 
CGAACTCCTT CGGGACAGCT TCGACTCTAC TGTAAAGGGG CTGATAACGT GATTTTTGAG 
AGACTTTCAA AAGATTCAAA ATACATGGAG GAGACTCTCT GCCACCTGGA GTACTTTGCC 
ACGGAAGGCT TGCGGACTCT GTGTGTGGCT TACGCCGACC TCAGTGAGCG GGATTACGAG 
GAGTGGCTCA AAGTCTACCA GGAAGCCAGC ACCATCCTGA AGGACCGCGC TCAGCGGCTG 
GAGGAGTGCT ACGAGATCAT CGAGAAGAAT CTACTGCTAC TTGGAGCCAC GGCCATAGAA 
GATCGACTTC AGGCAGGCGT GCCGGAAACC ATAGCAACTC TGTTGAAGGC CGAGATTAAA 
ATATGGGTGT TGACAGGCGA CAAACAAGAA ACTGCTATTA ATATAGGTTA TTCCTGTCGG 
TTGGTGTCAC AGAACATGGC CCTTATCCTA CTGAAGGAGG ATTCTTTGGA CGCCACCCGG 
GCAGCCATCA CCCAGCACTG CGCGGACCTC GGGAGCCTGC TGGGCAAGGA GAACGACGCG 
GCCCTCATCA TCGACGGCCA CACCCTCAAG TACGCGCTGT CCTTCGAGGT CCGGAGGAGC 
TTCCTGGACC TGGCCCTCTC GTGCAAGGCA GTCATCTGTT GCAGGGTGTC TCCTCTGCAG 
AAGTCGGAGA TAGTGGATGT GGTGAAGAAG CGGGTGAAAG CCATCACCCT GGCCATTGGG 
GATGGTGCCA ACGATGTGGG CATGATCCAG ACGGCACACG TGGGTGTAGG CATCAGTGGG 
AACGAGGGCA TGCAGGCCAC TAACAACTCG GATTACGCCA TCGCTCAGTT TTCCTACTTG 
GAGAAGCTCC TGCTGGTTCA CGGCGCCTGG AGCTACAACC GGGTGACCAA GTGCATCTTG 
TACTGTTTCT ACAAGAACGT GGTGCTCTAC ATCATTGAGC TTTGGTTTGC CTTCGTTAAT 
GGATTTTCTG GGCAGATTTT ATTTGAACGT TGGTGCATCG GCTTGTACAA CGTGATTTTC 
ACGGCACTGC CCCCCTTCAC GCTGGGCATC TTCGAGAGGT CTTGCAGCCA GGAGAGCATG 
CTGAGGTTCC CGCAGCTCTA CAAGATCACC CAGAACGCCG AGGGCTTCAA CACCAAGGTT 
TTCTGGGGTC ACTGCATCAA CGCCTTGGTC CACTCACTCA TCCTGTTCTG GTTTCCCATG 
AAAGCGCTGG AGCATGATAC TGTGTTGGCC AATGGTCATG CCACAGACTA TTTATTTGTT 
GGAAATATCG TTTATACTTA TGTTGTTGTT ACTGTTTGTC TGAAAGCTGG TTTGGAGACG 
ACAGCTTGGA CCAAATTCAG TCACCTGGCC GTCTGGGGAA GCATGCTCAT CTGGCTGGTG 
TTCTTTGGCA TCTACTCGAC CATCTGGCCC ACCATTCCCA TCGCTCCAGA CATGAAAGGG 
CAGGCAACCA TGGTTCTGAG CTCTGCACAC TTCTGGCTGG GACTATTTCT GGTTCCCACC 
GCATGTCTGA TCGAAGATGT GGCATGGAGA GCGGCCAAGC ACACCTGCAA GAAAACGCTT 
CTGGAGGAAG TGCAGGAGCT GGAGATGAAG TCCAGAGTGA TGGGCAGAGC AATGCTCCGG 
GACAGTAACG GAAAGAGGAT GAACGAGCGA GACCGGCTGC TAAAGAGGCT GAGCAGGAAG 
ACGCCACCCA CCCTCTTCCG GGGCAGCTCC CTCCAGCAGT CCATGCCACA TGGGTATGCC 
TTCTCCCAGG AGGAACACGG AGCCGTGACT CAGGAGGAAA TCGTCCGCGC TTACGACACC 
ACGAAGCAGA AATCCAGGAA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ATP8A2 ( NM_001163802.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001157274.3 Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), mRNA.

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CloneID	OBa02292
Clone ID Related Accession (Same CDS sequence)	NM_001163802.3
Accession Version	NM_001163802.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Bos taurus(cattle)
Product	phospholipid-transporting ATPase IB
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from GQ303567.3. On Jan 25, 2012 this sequence version replaced NM_001163802.2. ##Evidence-Data-START## Transcript exon combination :: GQ303567.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145414 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCTGG CCCACAGCTG GAGAAGATAC TGCTCTATAG GACCTGTCCG TCCTCCCCCG 
GGCTACAAGA AGGCAGACGA TGAGATGTCC CGGGCCACGT CTGTTGGAGA CCAGCTGGAC 
GTACCAGCCC GCACCATTTA CCTCAACCAA CCGCATCTCA ACAAATTCTG CGACAACCAG 
ATCAGCACGG CCAAGTACAG CGTGGTGACG TTTCTACCTC GATTCTTGTA CGAGCAGATC 
AGGAGAGCTG CCAATGCCTT CTTCCTCTTC ATTGCCTTAT TACAGCAAAT TCCAGATGTA 
TCTCCAACAG GAAGATACAC CACCCTGGTG CCATTGATCA TTATTTTAAC AATCGCAGGC 
ATCAAAGAGA TTGTAGAAGA TTTTAAGCGA CATAAGGCAG ACAATGCAGT TAACAAAAAG 
AAAACCATAG TGTTAAGGAA TGGTATGTGG CAAACCATTG TGTGGAAAGA GGTGGCCGTG 
GGAGACATCG TCAAAGTCGT CAACGGGCAG TACCTTCCAG CAGACGTGGT CCTGCTCTCC 
TCCAGTGAAC CTCAGGCAAT GTGTTACGTT GAAACAGCCA ATCTGGACGG GGAGACGAAC 
CTTAAAATAC GTCAGGGTTT GAGTCACACT GCGGACATGC AGACGAGGGA GGTGCTGATG 
AAGTTGTCTG GGACCATAGA GTGCGAAGGG CCCAATCGCC ACCTCTACGA CTTCACCGGG 
AACTTGAACT TGGATGGGAA AAGCCCCGTT GCCCTTGGGC CGGACCAGAT CTTATTGAGA 
GGCACACAGC TTAGAAACAC TCAGTGGGGC TTTGGCATAG TTGTCTACAC TGGACATGAC 
ACCAAGCTCA TGCAGAATTC CACCAAAGCC CCTCTCAAGA GGTCGAATGT GGAGAAGGTA 
ACCAACGTGC AGATCCTGGT ACTGTTCGGC ATCCTGCTGG TCATGGCCCT GGTGAGCTCG 
GTGGGAGCCC TATACTGGAA CGGCTCTCAA GGAGGGAAGA ACTGGTACAT CAAGAAGATG 
GATGCGACCT CAGACAATTT CGGATACAAC CTGTTGACAT TCATCATCCT CTACAACAAT 
CTGATTCCCA TCAGTCTGCT GGTGACTCTC GAGGTTGTGA AGTACACACA AGCCCTCTTC 
ATAAACTGGG ACACAGATAT GTATTATCTA GGAAATGACA CCCCTGCCAT GGCCAGGACG 
TCAAACCTTA ATGAAGAGCT TGGCCAGGTA AAATATCTAT TTTCTGACAA AACAGGAACT 
CTGACATGCA ATATCATGAA CTTCAAAAAG TGTAGCATTG CTGGGGTAAC CTATGGTCAT 
TTTCCAGAAC TGACAAGGGA ACCATCCTCA GATGATTTCT CCCGGATTCC TCCTCCACCT 
AGTGATTCCT GCGACTTTGA TGACCCCAGG CTCTTGAAGA ACATCGAGGA TCATCATCCC 
ACAGCACCCT GCATCCAGGA GTTCCTGACC CTGCTGGCCG TGTGCCACAC CGTGGTCCCC 
GAGAGGGACG GGGACAGCAT CGTCTACCAG GCCTCCTCCC CAGACGAAGC CGCTTTGGTC 
AAAGGGGCCC GGAAGCTGGG CTTTGTCTTC ACAGCCAGAA CGCCCTACTC TGTCATCATC 
GAGGCCATGG GACAGGAACA GACATTTGGA ATCCTGAATG TCCTGGAATT CTCCAGCGAC 
AGAAAGAGAA TGTCTGTGAT AGTGCGAACT CCTTCGGGAC AGCTTCGACT CTACTGTAAA 
GGGGCTGATA ACGTGATTTT TGAGAGACTT TCAAAAGATT CAAAATACAT GGAGGAGACT 
CTCTGCCACC TGGAGTACTT TGCCACGGAA GGCTTGCGGA CTCTGTGTGT GGCTTACGCC 
GACCTCAGTG AGCGGGATTA CGAGGAGTGG CTCAAAGTCT ACCAGGAAGC CAGCACCATC 
CTGAAGGACC GCGCTCAGCG GCTGGAGGAG TGCTACGAGA TCATCGAGAA GAATCTACTG 
CTACTTGGAG CCACGGCCAT AGAAGATCGA CTTCAGGCAG GCGTGCCGGA AACCATAGCA 
ACTCTGTTGA AGGCCGAGAT TAAAATATGG GTGTTGACAG GCGACAAACA AGAAACTGCT 
ATTAATATAG GTTATTCCTG TCGGTTGGTG TCACAGAACA TGGCCCTTAT CCTACTGAAG 
GAGGATTCTT TGGACGCCAC CCGGGCAGCC ATCACCCAGC ACTGCGCGGA CCTCGGGAGC 
CTGCTGGGCA AGGAGAACGA CGCGGCCCTC ATCATCGACG GCCACACCCT CAAGTACGCG 
CTGTCCTTCG AGGTCCGGAG GAGCTTCCTG GACCTGGCCC TCTCGTGCAA GGCAGTCATC 
TGTTGCAGGG TGTCTCCTCT GCAGAAGTCG GAGATAGTGG ATGTGGTGAA GAAGCGGGTG 
AAAGCCATCA CCCTGGCCAT CGGGGATGGT GCCAACGATG TGGGCATGAT CCAGACGGCA 
CACGTGGGTG TAGGCATCAG TGGGAACGAG GGCATGCAGG CCACTAACAA CTCGGATTAC 
GCCATCGCTC AGTTTTCCTA CTTGGAGAAG CTCCTGCTGG TTCACGGCGC CTGGAGCTAC 
AACCGGGTGA CCAAGTGCAT CTTGTACTGT TTCTACAAGA ACGTGGTGCT CTACATCATT 
GAGCTTTGGT TTGCCTTCGT TAATGGATTT TCTGGGCAGA TTTTATTTGA ACGTTGGTGC 
ATCGGCTTGT ACAACGTGAT TTTCACGGCA CTGCCCCCCT TCACGCTGGG CATCTTTGAG 
AGGTCTTGCA GCCAGGAGAG CATGCTGAGG TTCCCGCAGC TCTACAAGAT CACCCAGAAC 
GCCGAGGGCT TCAACACCAA GGTTTTCTGG GGTCACTGCA TCAACGCCTT GGTCCACTCA 
CTCATCCTGT TCTGGTTTCC CATGAAAGCG CTGGAGCATG ATACTGTGTT GGCCAATGGT 
CATGCCACAG ACTATTTATT TGTTGGAAAT ATCGTTTATA CTTATGTTGT TGTTACTGTT 
TGTCTGAAAG CTGGTTTGGA GACGACAGCT TGGACCAAAT TCAGTCACCT GGCCGTCTGG 
GGAAGCATGC TCATCTGGCT GGTGTTCTTT GGCATCTACT CGACCATCTG GCCCACCATT 
CCCATCGCTC CAGACATGAA AGGACAGGCA ACCATGGTTC TGAGCTCTGC ACACTTCTGG 
CTGGGACTAT TTCTGGTTCC CACCGCATGT CTGATCGAAG ATGTGGCATG GAGAGCGGCC 
AAGCACACCT GCAAGAAAAC GCTTCTGGAG GAAGTGCAGG AGCTGGAGAT GAAGTCCAGA 
GTGATGGGCA GAGCAATGCT CCGGGACAGT AACGGAAAGA GGATGAACGA GCGAGACCGG 
CTGCTAAAGA GGCTGAGCAG GAAGACGCCA CCCACCCTCT TCCGGGGCAG CTCCCTCCAG 
CAGTCCATGC CACATGGGTA TGCCTTCTCC CAGGAGGAAC ACGGAGCCGT GACTCAGGAG 
GAAATCGTCC GCGCTTACGA CACCACGAAG CAGAAATCCA GGAAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157274.3
CDS	35..3565
Translation	MPLAHSWRRYCSIGPVRPPPGYKKADDEMSRATSVGDQLDVPARTIYLNQPHLNKFCDNQISTAKYSVVTFLPRFLYEQIRRAANAFFLFIALLQQIPDVSPTGRYTTLVPLIIILTIAGIKEIVEDFKRHKADNAVNKKKTIVLRNGMWQTIVWKEVAVGDIVKVVNGQYLPADVVLLSSSEPQAMCYVETANLDGETNLKIRQGLSHTADMQTREVLMKLSGTIECEGPNRHLYDFTGNLNLDGKSPVALGPDQILLRGTQLRNTQWGFGIVVYTGHDTKLMQNSTKAPLKRSNVEKVTNVQILVLFGILLVMALVSSVGALYWNGSQGGKNWYIKKMDATSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQALFINWDTDMYYLGNDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELTREPSSDDFSRIPPPPSDSCDFDDPRLLKNIEDHHPTAPCIQEFLTLLAVCHTVVPERDGDSIVYQASSPDEAALVKGARKLGFVFTARTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRTPSGQLRLYCKGADNVIFERLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSERDYEEWLKVYQEASTILKDRAQRLEECYEIIEKNLLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATRAAITQHCADLGSLLGKENDAALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKKRVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCILYCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCSQESMLRFPQLYKITQNAEGFNTKVFWGHCINALVHSLILFWFPMKALEHDTVLANGHATDYLFVGNIVYTYVVVTVCLKAGLETTAWTKFSHLAVWGSMLIWLVFFGIYSTIWPTIPIAPDMKGQATMVLSSAHFWLGLFLVPTACLIEDVAWRAAKHTCKKTLLEEVQELEMKSRVMGRAMLRDSNGKRMNERDRLLKRLSRKTPPTLFRGSSLQQSMPHGYAFSQEEHGAVTQEEIVRAYDTTKQKSRKK

Target ORF information:

RefSeq Version	NM_001163802.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ATPase, aminophospholipid transporter, class I, type 8A, member 2 (ATP8A2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163802.3

ORF Insert Sequence:

ATGCCTCTGG CCCACAGCTG GAGAAGATAC TGCTCTATAG GACCTGTCCG TCCTCCCCCG 
GGCTACAAGA AGGCAGACGA TGAGATGTCC CGGGCCACGT CTGTTGGAGA CCAGCTGGAC 
GTACCAGCCC GCACCATTTA CCTCAACCAA CCGCATCTCA ACAAATTCTG CGACAACCAG 
ATCAGCACGG CCAAGTACAG CGTGGTGACG TTTCTACCTC GATTCTTGTA CGAGCAGATC 
AGGAGAGCTG CCAATGCCTT CTTCCTCTTC ATTGCCTTAT TACAGCAAAT TCCAGATGTA 
TCTCCAACAG GAAGATACAC CACCCTGGTG CCATTGATCA TTATTTTAAC AATCGCAGGC 
ATCAAAGAGA TTGTAGAAGA TTTTAAGCGA CATAAGGCAG ACAATGCAGT TAACAAAAAG 
AAAACCATAG TGTTAAGGAA TGGTATGTGG CAAACCATTG TGTGGAAAGA GGTGGCCGTG 
GGAGACATCG TCAAAGTCGT CAACGGGCAG TACCTTCCAG CAGACGTGGT CCTGCTCTCC 
TCCAGTGAAC CTCAGGCAAT GTGTTACGTT GAAACAGCCA ATCTGGACGG GGAGACGAAC 
CTTAAAATAC GTCAGGGTTT GAGTCACACT GCGGACATGC AGACGAGGGA GGTGCTGATG 
AAGTTGTCTG GGACCATAGA GTGCGAAGGG CCCAATCGCC ACCTCTACGA CTTCACCGGG 
AACTTGAACT TGGATGGGAA AAGCCCCGTT GCCCTTGGGC CGGACCAGAT CTTATTGAGA 
GGCACACAGC TTAGAAACAC TCAGTGGGGC TTTGGCATAG TTGTCTACAC TGGACATGAC 
ACCAAGCTCA TGCAGAATTC CACCAAAGCC CCTCTCAAGA GGTCGAATGT GGAGAAGGTA 
ACCAACGTGC AGATCCTGGT ACTGTTCGGC ATCCTGCTGG TCATGGCCCT GGTGAGCTCG 
GTGGGAGCCC TATACTGGAA CGGCTCTCAA GGAGGGAAGA ACTGGTACAT CAAGAAGATG 
GATGCGACCT CAGACAATTT CGGATACAAC CTGTTGACAT TCATCATCCT CTACAACAAT 
CTGATTCCCA TCAGTCTGCT GGTGACTCTC GAGGTTGTGA AGTACACACA AGCCCTCTTC 
ATAAACTGGG ACACAGATAT GTATTATCTA GGAAATGACA CCCCTGCCAT GGCCAGGACG 
TCAAACCTTA ATGAAGAGCT TGGCCAGGTA AAATATCTAT TTTCTGACAA AACAGGAACT 
CTGACATGCA ATATCATGAA CTTCAAAAAG TGTAGCATTG CTGGGGTAAC CTATGGTCAT 
TTTCCAGAAC TGACAAGGGA ACCATCCTCA GATGATTTCT CCCGGATTCC TCCTCCACCT 
AGTGATTCCT GCGACTTTGA TGACCCCAGG CTCTTGAAGA ACATCGAGGA TCATCATCCC 
ACAGCACCCT GCATCCAGGA GTTCCTGACC CTGCTGGCCG TGTGCCACAC CGTGGTCCCC 
GAGAGGGACG GGGACAGCAT CGTCTACCAG GCCTCCTCCC CAGACGAAGC CGCTTTGGTC 
AAAGGGGCCC GGAAGCTGGG CTTTGTCTTC ACAGCCAGAA CGCCCTACTC TGTCATCATC 
GAGGCCATGG GACAGGAACA GACATTTGGA ATCCTGAATG TCCTGGAATT CTCCAGCGAC 
AGAAAGAGAA TGTCTGTGAT AGTGCGAACT CCTTCGGGAC AGCTTCGACT CTACTGTAAA 
GGGGCTGATA ACGTGATTTT TGAGAGACTT TCAAAAGATT CAAAATACAT GGAGGAGACT 
CTCTGCCACC TGGAGTACTT TGCCACGGAA GGCTTGCGGA CTCTGTGTGT GGCTTACGCC 
GACCTCAGTG AGCGGGATTA CGAGGAGTGG CTCAAAGTCT ACCAGGAAGC CAGCACCATC 
CTGAAGGACC GCGCTCAGCG GCTGGAGGAG TGCTACGAGA TCATCGAGAA GAATCTACTG 
CTACTTGGAG CCACGGCCAT AGAAGATCGA CTTCAGGCAG GCGTGCCGGA AACCATAGCA 
ACTCTGTTGA AGGCCGAGAT TAAAATATGG GTGTTGACAG GCGACAAACA AGAAACTGCT 
ATTAATATAG GTTATTCCTG TCGGTTGGTG TCACAGAACA TGGCCCTTAT CCTACTGAAG 
GAGGATTCTT TGGACGCCAC CCGGGCAGCC ATCACCCAGC ACTGCGCGGA CCTCGGGAGC 
CTGCTGGGCA AGGAGAACGA CGCGGCCCTC ATCATCGACG GCCACACCCT CAAGTACGCG 
CTGTCCTTCG AGGTCCGGAG GAGCTTCCTG GACCTGGCCC TCTCGTGCAA GGCAGTCATC 
TGTTGCAGGG TGTCTCCTCT GCAGAAGTCG GAGATAGTGG ATGTGGTGAA GAAGCGGGTG 
AAAGCCATCA CCCTGGCCAT CGGGGATGGT GCCAACGATG TGGGCATGAT CCAGACGGCA 
CACGTGGGTG TAGGCATCAG TGGGAACGAG GGCATGCAGG CCACTAACAA CTCGGATTAC 
GCCATCGCTC AGTTTTCCTA CTTGGAGAAG CTCCTGCTGG TTCACGGCGC CTGGAGCTAC 
AACCGGGTGA CCAAGTGCAT CTTGTACTGT TTCTACAAGA ACGTGGTGCT CTACATCATT 
GAGCTTTGGT TTGCCTTCGT TAATGGATTT TCTGGGCAGA TTTTATTTGA ACGTTGGTGC 
ATCGGCTTGT ACAACGTGAT TTTCACGGCA CTGCCCCCCT TCACGCTGGG CATCTTTGAG 
AGGTCTTGCA GCCAGGAGAG CATGCTGAGG TTCCCGCAGC TCTACAAGAT CACCCAGAAC 
GCCGAGGGCT TCAACACCAA GGTTTTCTGG GGTCACTGCA TCAACGCCTT GGTCCACTCA 
CTCATCCTGT TCTGGTTTCC CATGAAAGCG CTGGAGCATG ATACTGTGTT GGCCAATGGT 
CATGCCACAG ACTATTTATT TGTTGGAAAT ATCGTTTATA CTTATGTTGT TGTTACTGTT 
TGTCTGAAAG CTGGTTTGGA GACGACAGCT TGGACCAAAT TCAGTCACCT GGCCGTCTGG 
GGAAGCATGC TCATCTGGCT GGTGTTCTTT GGCATCTACT CGACCATCTG GCCCACCATT 
CCCATCGCTC CAGACATGAA AGGACAGGCA ACCATGGTTC TGAGCTCTGC ACACTTCTGG 
CTGGGACTAT TTCTGGTTCC CACCGCATGT CTGATCGAAG ATGTGGCATG GAGAGCGGCC 
AAGCACACCT GCAAGAAAAC GCTTCTGGAG GAAGTGCAGG AGCTGGAGAT GAAGTCCAGA 
GTGATGGGCA GAGCAATGCT CCGGGACAGT AACGGAAAGA GGATGAACGA GCGAGACCGG 
CTGCTAAAGA GGCTGAGCAG GAAGACGCCA CCCACCCTCT TCCGGGGCAG CTCCCTCCAG 
CAGTCCATGC CACATGGGTA TGCCTTCTCC CAGGAGGAAC ACGGAGCCGT GACTCAGGAG 
GAAATCGTCC GCGCTTACGA CACCACGAAG CAGAAATCCA GGAAGAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Specific mutations in mammalian P4-ATPase ATP8A2 catalytic subunit entail differential glycosylation of the accessory CDC50A subunit.
FEBS letters589(24 Pt B)3908-14(2015 Dec)
Vestergaard AL,Mikkelsen SA,Coleman JA,Molday RS,Vilsen B,Andersen JP

Critical role of the beta-subunit CDC50A in the stable expression, assembly, subcellular localization, and lipid transport activity of the P4-ATPase ATP8A2.
The Journal of biological chemistry286(19)17205-16(2011 May)
Coleman JA,Molday RS

Localization, purification, and functional reconstitution of the P4-ATPase Atp8a2, a phosphatidylserine flippase in photoreceptor disc membranes.
The Journal of biological chemistry284(47)32670-9(2009 Nov)
Coleman JA,Kwok MC,Molday RS

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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ATP8A2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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