EMC1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMC1
Entrez Gene ID	508439
Full Name	ER membrane protein complex subunit 1
General protein information	Preferred Names ER membrane protein complex subunit 1 Names ER membrane protein complex subunit 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005203354.3	XP_005203411.2	ER membrane protein complex subunit 1 isoform X1
XM_005203355.3	XP_005203412.2	ER membrane protein complex subunit 1 isoform X2
XM_005197643.3	XP_005197700.2	ER membrane protein complex subunit 1 isoform X2
XM_024983204.1	XP_024838972.1	ER membrane protein complex subunit 1 isoform X3
XM_005203354.4	XP_005203411.2	ER membrane protein complex subunit 1 isoform X1
XM_005203355.4	XP_005203412.3	ER membrane protein complex subunit 1 isoform X2
XM_005197642.3	XP_005197699.2	ER membrane protein complex subunit 1 isoform X1

Pathways from BioSystems

Homologies

	EMC1	XP_001091700.1
Canis lupus familiaris (dog)	EMC1	XP_005617944.1
Drosophila melanogaster (fruit fly)	CG2943	NP_649747.1
Mus musculus (house mouse)	Emc1	NP_666269.2
Arabidopsis thaliana (thale cress)	AT5G11560	NP_196717.3
Bos taurus (cattle)	EMC1	XP_005203411.1
Danio rerio (zebrafish)	emc1	XP_693770.3
Caenorhabditis elegans (roundworm)	H17B01.4	NP_493980.1
Oryza sativa (rice)	Os05g0230600	NP_001054975.1
Gallus gallus (chicken)	EMC1	NP_001012856.1
Homo sapiens (human)	EMC1	NP_055862.1
Pan troglodytes (chimpanzee)	EMC1	XP_001162981.2
Rattus norvegicus (Norway rat)	Emc1	NP_001102160.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003647	XP_562511.3
Xenopus tropicalis (tropical clawed frog)	emc1	XP_002943252.2

Gene Ontology

The following EMC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa47142	XM_005197642.3 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47142	XM_005203354.4 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47143	XM_005203355.3 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa47143	XM_005197643.3 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa227932	XM_024983204.1 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OBa227931	XM_005203355.4 Latest version!	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OBa47142	XM_005203354.3	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMC1 ( XM_005203354.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203411.2 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47142
Clone ID Related Accession (Same CDS sequence)	XM_005203354.3 , XM_005203354.4 , XM_005197642.3
Accession Version	XM_005203354.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203354.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203411.2
CDS	46..3033
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005203354.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203354.3

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005203354.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203411.2 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47142
Clone ID Related Accession (Same CDS sequence)	XM_005203354.3 , XM_005203354.4 , XM_005197642.3
Accession Version	XM_005203354.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203354.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203411.2
CDS	64..3051
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005203354.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203354.4

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005197642.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197699.2 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa47142
Clone ID Related Accession (Same CDS sequence)	XM_005203354.3 , XM_005203354.4 , XM_005197642.3
Accession Version	XM_005197642.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197642.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197699.2
CDS	46..3033
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKAAIQDGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005197642.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197642.3

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGC CATCCAAGAC 
GGCCTGCTGG GCATGTTCCT GAAGCGCCTG TCCTCCCAGC TCATCCTGCT GCAGGCCTGG 
ACCTCCCACC TCTGGAAAGT GTTTTACGAT GCGCGGAAGC CCCGGAGCCA GATCAAGAAC 
GAGATCAACA TTGACACTCT GGCCAGGGAT GAATTCAACC TCCAGAAGAT GATGGTGATG 
GTGACGGCCT CGGGCAAGCT TTTTGGCATC GAGAGCAGCT CTGGCACCAT CCTGTGGAAA 
CAGTATCTCC CGAACGTCAA GCCGGACTCC TCCTTTGAGC TGACGGTCCA GAGGACGACT 
GCCCACTTCC CCCACCCGCC CCAGTGCACG CTCCTGCTGA AAGACAAGGA GACGGGAATG 
AGCTCGCTGT ATGTCTTCAA CCCCATTTTT GGGAAGTGGA GCCAGGTGGC CCCCCCCGTG 
CTGAAGCGCC CCGTCTTGCA GTCTCTGCTC CTGCCCATCA TGGATCAGGA CTATGCCAAG 
GTGCTGTTGC TGATAGACGA CGAGTACAAG GTCACAGCTT TCCCAGCCAC TCGGAATGTC 
TTGCGGCAGC TCCATGAGCT CGCCCCTTCC ATCTTCTTCT ACTTGGTGGA CGCGGATCAG 
GGGCGGCTGT GCGGATATCG ACTCCGGAAG GACCTCACCA CTGAGCTGAG CTGGGAGCTG 
ACCATCCCCC CGGAGGTGCA GCGAATCGTC CAGGTGAAGG GGAAGCGCAG CAGCGAGCAT 
GTCCACTCGC AGGGCCGAGT GATGGGGGAC CGCAGCGTGC TCTACAAGAG CCTGAACCCC 
AACCTGCTGG CCGTGGTGAC GGAGAGCACG GACGTCCACC ACGAGCGCAC CTTCATCGGC 
GTCTTCCTCG TGGACGGCGT CACGGGGCGC ATCGTCCACT CGTCCGTGCA GAGGAAGGCC 
AAGGGCCCCG TCCACATCGT GCACGCCGAG AACTGGGTTG CGTACCAGTA CTGGAACACC 
AAGGCTCGGC GCAACGAGTT CACGGCGCTG GAGCTCTACG AGGGCACGGA GCAGTACAAT 
GCCACAGCCT TCAGCTCCCT GGACCGCCCG CAGCTGCCCC AGGTCCTGCA GCAGTCCTAC 
ATCTTCCCCT CCGCCATCAG CGCCATGGAG GCCACCATCA CAGAGCGGGG CATCACCAGC 
CGCCACCTGC TCGTTGGGCT GCCTTCTGGA GCGATCCTTT CCCTTCCTAA GGCCTTGCTG 
GATCCTCGGC GCCCCGAGAT CCCAACAGAA CAGAGCAGAG AGGAGAACCT GATCCCGTAT 
TCTCCAGACG TGCAGATCCA CGCAGAGCGG TTCATCAACT ATAACCAGAC AGTCTCCCGA 
ATGCGAGGCA TCTACACAGC CCCGTCGGGC CTGGAGTCCA CTTGTCTGGT CGTGGCCTAC 
GGTTTGGACA TTTACCAGAC CCGCGTCTAC CCGTCCAAAC AGTTCGACGT CCTGAAGGAC 
GACTACGACT ACGTGCTCAT CAGCAGCGTC CTCTTTGGCC TGGTCTTTGC CACCATGATC 
ACGAAGAGGC TGGCGCAGGT CAAACTCCTG AACCGTGCCT GGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005203355.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203412.2 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa47143
Clone ID Related Accession (Same CDS sequence)	XM_005203355.3 , XM_005197643.3
Accession Version	XM_005203355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103851.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGA CGGCCTGCTG 
GGCATGTTCC TGAAGCGCCT GTCCTCCCAG CTCATCCTGC TGCAGGCCTG GACCTCCCAC 
CTCTGGAAAG TGTTTTACGA TGCGCGGAAG CCCCGGAGCC AGATCAAGAA CGAGATCAAC 
ATTGACACTC TGGCCAGGGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTGACGGCC 
TCGGGCAAGC TTTTTGGCAT CGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTC 
CCGAACGTCA AGCCGGACTC CTCCTTTGAG CTGACGGTCC AGAGGACGAC TGCCCACTTC 
CCCCACCCGC CCCAGTGCAC GCTCCTGCTG AAAGACAAGG AGACGGGAAT GAGCTCGCTG 
TATGTCTTCA ACCCCATTTT TGGGAAGTGG AGCCAGGTGG CCCCCCCCGT GCTGAAGCGC 
CCCGTCTTGC AGTCTCTGCT CCTGCCCATC ATGGATCAGG ACTATGCCAA GGTGCTGTTG 
CTGATAGACG ACGAGTACAA GGTCACAGCT TTCCCAGCCA CTCGGAATGT CTTGCGGCAG 
CTCCATGAGC TCGCCCCTTC CATCTTCTTC TACTTGGTGG ACGCGGATCA GGGGCGGCTG 
TGCGGATATC GACTCCGGAA GGACCTCACC ACTGAGCTGA GCTGGGAGCT GACCATCCCC 
CCGGAGGTGC AGCGAATCGT CCAGGTGAAG GGGAAGCGCA GCAGCGAGCA TGTCCACTCG 
CAGGGCCGAG TGATGGGGGA CCGCAGCGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CGGAGAGCAC GGACGTCCAC CACGAGCGCA CCTTCATCGG CGTCTTCCTC 
GTGGACGGCG TCACGGGGCG CATCGTCCAC TCGTCCGTGC AGAGGAAGGC CAAGGGCCCC 
GTCCACATCG TGCACGCCGA GAACTGGGTT GCGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TCACGGCGCT GGAGCTCTAC GAGGGCACGG AGCAGTACAA TGCCACAGCC 
TTCAGCTCCC TGGACCGCCC GCAGCTGCCC CAGGTCCTGC AGCAGTCCTA CATCTTCCCC 
TCCGCCATCA GCGCCATGGA GGCCACCATC ACAGAGCGGG GCATCACCAG CCGCCACCTG 
CTCGTTGGGC TGCCTTCTGG AGCGATCCTT TCCCTTCCTA AGGCCTTGCT GGATCCTCGG 
CGCCCCGAGA TCCCAACAGA ACAGAGCAGA GAGGAGAACC TGATCCCGTA TTCTCCAGAC 
GTGCAGATCC ACGCAGAGCG GTTCATCAAC TATAACCAGA CAGTCTCCCG AATGCGAGGC 
ATCTACACAG CCCCGTCGGG CCTGGAGTCC ACTTGTCTGG TCGTGGCCTA CGGTTTGGAC 
ATTTACCAGA CCCGCGTCTA CCCGTCCAAA CAGTTCGACG TCCTGAAGGA CGACTACGAC 
TACGTGCTCA TCAGCAGCGT CCTCTTTGGC CTGGTCTTTG CCACCATGAT CACGAAGAGG 
CTGGCGCAGG TCAAACTCCT GAACCGTGCC TGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203412.2
CDS	46..3024
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005203355.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203355.3

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGA CGGCCTGCTG 
GGCATGTTCC TGAAGCGCCT GTCCTCCCAG CTCATCCTGC TGCAGGCCTG GACCTCCCAC 
CTCTGGAAAG TGTTTTACGA TGCGCGGAAG CCCCGGAGCC AGATCAAGAA CGAGATCAAC 
ATTGACACTC TGGCCAGGGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTGACGGCC 
TCGGGCAAGC TTTTTGGCAT CGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTC 
CCGAACGTCA AGCCGGACTC CTCCTTTGAG CTGACGGTCC AGAGGACGAC TGCCCACTTC 
CCCCACCCGC CCCAGTGCAC GCTCCTGCTG AAAGACAAGG AGACGGGAAT GAGCTCGCTG 
TATGTCTTCA ACCCCATTTT TGGGAAGTGG AGCCAGGTGG CCCCCCCCGT GCTGAAGCGC 
CCCGTCTTGC AGTCTCTGCT CCTGCCCATC ATGGATCAGG ACTATGCCAA GGTGCTGTTG 
CTGATAGACG ACGAGTACAA GGTCACAGCT TTCCCAGCCA CTCGGAATGT CTTGCGGCAG 
CTCCATGAGC TCGCCCCTTC CATCTTCTTC TACTTGGTGG ACGCGGATCA GGGGCGGCTG 
TGCGGATATC GACTCCGGAA GGACCTCACC ACTGAGCTGA GCTGGGAGCT GACCATCCCC 
CCGGAGGTGC AGCGAATCGT CCAGGTGAAG GGGAAGCGCA GCAGCGAGCA TGTCCACTCG 
CAGGGCCGAG TGATGGGGGA CCGCAGCGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CGGAGAGCAC GGACGTCCAC CACGAGCGCA CCTTCATCGG CGTCTTCCTC 
GTGGACGGCG TCACGGGGCG CATCGTCCAC TCGTCCGTGC AGAGGAAGGC CAAGGGCCCC 
GTCCACATCG TGCACGCCGA GAACTGGGTT GCGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TCACGGCGCT GGAGCTCTAC GAGGGCACGG AGCAGTACAA TGCCACAGCC 
TTCAGCTCCC TGGACCGCCC GCAGCTGCCC CAGGTCCTGC AGCAGTCCTA CATCTTCCCC 
TCCGCCATCA GCGCCATGGA GGCCACCATC ACAGAGCGGG GCATCACCAG CCGCCACCTG 
CTCGTTGGGC TGCCTTCTGG AGCGATCCTT TCCCTTCCTA AGGCCTTGCT GGATCCTCGG 
CGCCCCGAGA TCCCAACAGA ACAGAGCAGA GAGGAGAACC TGATCCCGTA TTCTCCAGAC 
GTGCAGATCC ACGCAGAGCG GTTCATCAAC TATAACCAGA CAGTCTCCCG AATGCGAGGC 
ATCTACACAG CCCCGTCGGG CCTGGAGTCC ACTTGTCTGG TCGTGGCCTA CGGTTTGGAC 
ATTTACCAGA CCCGCGTCTA CCCGTCCAAA CAGTTCGACG TCCTGAAGGA CGACTACGAC 
TACGTGCTCA TCAGCAGCGT CCTCTTTGGC CTGGTCTTTG CCACCATGAT CACGAAGAGG 
CTGGCGCAGG TCAAACTCCT GAACCGTGCC TGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005197643.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005197700.2 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa47143
Clone ID Related Accession (Same CDS sequence)	XM_005203355.3 , XM_005197643.3
Accession Version	XM_005197643.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2979bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644620.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005197643.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGA CGGCCTGCTG 
GGCATGTTCC TGAAGCGCCT GTCCTCCCAG CTCATCCTGC TGCAGGCCTG GACCTCCCAC 
CTCTGGAAAG TGTTTTACGA TGCGCGGAAG CCCCGGAGCC AGATCAAGAA CGAGATCAAC 
ATTGACACTC TGGCCAGGGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTGACGGCC 
TCGGGCAAGC TTTTTGGCAT CGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTC 
CCGAACGTCA AGCCGGACTC CTCCTTTGAG CTGACGGTCC AGAGGACGAC TGCCCACTTC 
CCCCACCCGC CCCAGTGCAC GCTCCTGCTG AAAGACAAGG AGACGGGAAT GAGCTCGCTG 
TATGTCTTCA ACCCCATTTT TGGGAAGTGG AGCCAGGTGG CCCCCCCCGT GCTGAAGCGC 
CCCGTCTTGC AGTCTCTGCT CCTGCCCATC ATGGATCAGG ACTATGCCAA GGTGCTGTTG 
CTGATAGACG ACGAGTACAA GGTCACAGCT TTCCCAGCCA CTCGGAATGT CTTGCGGCAG 
CTCCATGAGC TCGCCCCTTC CATCTTCTTC TACTTGGTGG ACGCGGATCA GGGGCGGCTG 
TGCGGATATC GACTCCGGAA GGACCTCACC ACTGAGCTGA GCTGGGAGCT GACCATCCCC 
CCGGAGGTGC AGCGAATCGT CCAGGTGAAG GGGAAGCGCA GCAGCGAGCA TGTCCACTCG 
CAGGGCCGAG TGATGGGGGA CCGCAGCGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CGGAGAGCAC GGACGTCCAC CACGAGCGCA CCTTCATCGG CGTCTTCCTC 
GTGGACGGCG TCACGGGGCG CATCGTCCAC TCGTCCGTGC AGAGGAAGGC CAAGGGCCCC 
GTCCACATCG TGCACGCCGA GAACTGGGTT GCGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TCACGGCGCT GGAGCTCTAC GAGGGCACGG AGCAGTACAA TGCCACAGCC 
TTCAGCTCCC TGGACCGCCC GCAGCTGCCC CAGGTCCTGC AGCAGTCCTA CATCTTCCCC 
TCCGCCATCA GCGCCATGGA GGCCACCATC ACAGAGCGGG GCATCACCAG CCGCCACCTG 
CTCGTTGGGC TGCCTTCTGG AGCGATCCTT TCCCTTCCTA AGGCCTTGCT GGATCCTCGG 
CGCCCCGAGA TCCCAACAGA ACAGAGCAGA GAGGAGAACC TGATCCCGTA TTCTCCAGAC 
GTGCAGATCC ACGCAGAGCG GTTCATCAAC TATAACCAGA CAGTCTCCCG AATGCGAGGC 
ATCTACACAG CCCCGTCGGG CCTGGAGTCC ACTTGTCTGG TCGTGGCCTA CGGTTTGGAC 
ATTTACCAGA CCCGCGTCTA CCCGTCCAAA CAGTTCGACG TCCTGAAGGA CGACTACGAC 
TACGTGCTCA TCAGCAGCGT CCTCTTTGGC CTGGTCTTTG CCACCATGAT CACGAAGAGG 
CTGGCGCAGG TCAAACTCCT GAACCGTGCC TGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005197700.2
CDS	46..3024
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005197643.3
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005197643.3

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGCAGG GAGGGTGGTG 
CTGTGGAGCC GCGAGGAGTC CCTGGCCGAG GTGGTGTGCC TGGAGATGGT GGACCTGCCA 
CTGACCGGGG CACAGGCGGA GCTGGAAGGG GAGTTTGGCA AGAAGGCAGA CGGCCTGCTG 
GGCATGTTCC TGAAGCGCCT GTCCTCCCAG CTCATCCTGC TGCAGGCCTG GACCTCCCAC 
CTCTGGAAAG TGTTTTACGA TGCGCGGAAG CCCCGGAGCC AGATCAAGAA CGAGATCAAC 
ATTGACACTC TGGCCAGGGA TGAATTCAAC CTCCAGAAGA TGATGGTGAT GGTGACGGCC 
TCGGGCAAGC TTTTTGGCAT CGAGAGCAGC TCTGGCACCA TCCTGTGGAA ACAGTATCTC 
CCGAACGTCA AGCCGGACTC CTCCTTTGAG CTGACGGTCC AGAGGACGAC TGCCCACTTC 
CCCCACCCGC CCCAGTGCAC GCTCCTGCTG AAAGACAAGG AGACGGGAAT GAGCTCGCTG 
TATGTCTTCA ACCCCATTTT TGGGAAGTGG AGCCAGGTGG CCCCCCCCGT GCTGAAGCGC 
CCCGTCTTGC AGTCTCTGCT CCTGCCCATC ATGGATCAGG ACTATGCCAA GGTGCTGTTG 
CTGATAGACG ACGAGTACAA GGTCACAGCT TTCCCAGCCA CTCGGAATGT CTTGCGGCAG 
CTCCATGAGC TCGCCCCTTC CATCTTCTTC TACTTGGTGG ACGCGGATCA GGGGCGGCTG 
TGCGGATATC GACTCCGGAA GGACCTCACC ACTGAGCTGA GCTGGGAGCT GACCATCCCC 
CCGGAGGTGC AGCGAATCGT CCAGGTGAAG GGGAAGCGCA GCAGCGAGCA TGTCCACTCG 
CAGGGCCGAG TGATGGGGGA CCGCAGCGTG CTCTACAAGA GCCTGAACCC CAACCTGCTG 
GCCGTGGTGA CGGAGAGCAC GGACGTCCAC CACGAGCGCA CCTTCATCGG CGTCTTCCTC 
GTGGACGGCG TCACGGGGCG CATCGTCCAC TCGTCCGTGC AGAGGAAGGC CAAGGGCCCC 
GTCCACATCG TGCACGCCGA GAACTGGGTT GCGTACCAGT ACTGGAACAC CAAGGCTCGG 
CGCAACGAGT TCACGGCGCT GGAGCTCTAC GAGGGCACGG AGCAGTACAA TGCCACAGCC 
TTCAGCTCCC TGGACCGCCC GCAGCTGCCC CAGGTCCTGC AGCAGTCCTA CATCTTCCCC 
TCCGCCATCA GCGCCATGGA GGCCACCATC ACAGAGCGGG GCATCACCAG CCGCCACCTG 
CTCGTTGGGC TGCCTTCTGG AGCGATCCTT TCCCTTCCTA AGGCCTTGCT GGATCCTCGG 
CGCCCCGAGA TCCCAACAGA ACAGAGCAGA GAGGAGAACC TGATCCCGTA TTCTCCAGAC 
GTGCAGATCC ACGCAGAGCG GTTCATCAAC TATAACCAGA CAGTCTCCCG AATGCGAGGC 
ATCTACACAG CCCCGTCGGG CCTGGAGTCC ACTTGTCTGG TCGTGGCCTA CGGTTTGGAC 
ATTTACCAGA CCCGCGTCTA CCCGTCCAAA CAGTTCGACG TCCTGAAGGA CGACTACGAC 
TACGTGCTCA TCAGCAGCGT CCTCTTTGGC CTGGTCTTTG CCACCATGAT CACGAAGAGG 
CTGGCGCAGG TCAAACTCCT GAACCGTGCC TGGCGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_024983204.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838972.1 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa227932
Clone ID Related Accession (Same CDS sequence)	XM_024983204.1
Accession Version	XM_024983204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGACGG CCTGCTGGGC 
ATGTTCCTGA AGCGCCTGTC CTCCCAGCTC ATCCTGCTGC AGGCCTGGAC CTCCCACCTC 
TGGAAAGTGT TTTACGATGC GCGGAAGCCC CGGAGCCAGA TCAAGAACGA GATCAACATT 
GACACTCTGG CCAGGGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT GACGGCCTCG 
GGCAAGCTTT TTGGCATCGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTCCCG 
AACGTCAAGC CGGACTCCTC CTTTGAGCTG ACGGTCCAGA GGACGACTGC CCACTTCCCC 
CACCCGCCCC AGTGCACGCT CCTGCTGAAA GACAAGGAGA CGGGAATGAG CTCGCTGTAT 
GTCTTCAACC CCATTTTTGG GAAGTGGAGC CAGGTGGCCC CCCCCGTGCT GAAGCGCCCC 
GTCTTGCAGT CTCTGCTCCT GCCCATCATG GATCAGGACT ATGCCAAGGT GCTGTTGCTG 
ATAGACGACG AGTACAAGGT CACAGCTTTC CCAGCCACTC GGAATGTCTT GCGGCAGCTC 
CATGAGCTCG CCCCTTCCAT CTTCTTCTAC TTGGTGGACG CGGATCAGGG GCGGCTGTGC 
GGATATCGAC TCCGGAAGGA CCTCACCACT GAGCTGAGCT GGGAGCTGAC CATCCCCCCG 
GAGGTGCAGC GAATCGTCCA GGTGAAGGGG AAGCGCAGCA GCGAGCATGT CCACTCGCAG 
GGCCGAGTGA TGGGGGACCG CAGCGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACGG AGAGCACGGA CGTCCACCAC GAGCGCACCT TCATCGGCGT CTTCCTCGTG 
GACGGCGTCA CGGGGCGCAT CGTCCACTCG TCCGTGCAGA GGAAGGCCAA GGGCCCCGTC 
CACATCGTGC ACGCCGAGAA CTGGGTTGCG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTCA CGGCGCTGGA GCTCTACGAG GGCACGGAGC AGTACAATGC CACAGCCTTC 
AGCTCCCTGG ACCGCCCGCA GCTGCCCCAG GTCCTGCAGC AGTCCTACAT CTTCCCCTCC 
GCCATCAGCG CCATGGAGGC CACCATCACA GAGCGGGGCA TCACCAGCCG CCACCTGCTC 
GTTGGGCTGC CTTCTGGAGC GATCCTTTCC CTTCCTAAGG CCTTGCTGGA TCCTCGGCGC 
CCCGAGATCC CAACAGAACA GAGCAGAGAG GAGAACCTGA TCCCGTATTC TCCAGACGTG 
CAGATCCACG CAGAGCGGTT CATCAACTAT AACCAGACAG TCTCCCGAAT GCGAGGCATC 
TACACAGCCC CGTCGGGCCT GGAGTCCACT TGTCTGGTCG TGGCCTACGG TTTGGACATT 
TACCAGACCC GCGTCTACCC GTCCAAACAG TTCGACGTCC TGAAGGACGA CTACGACTAC 
GTGCTCATCA GCAGCGTCCT CTTTGGCCTG GTCTTTGCCA CCATGATCAC GAAGAGGCTG 
GCGCAGGTCA AACTCCTGAA CCGTGCCTGG CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838972.1
CDS	64..2919
Translation	MAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLDGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_024983204.1
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983204.1

ORF Insert Sequence:

ATGGCGGCGG CCTCGGTTTC CCGGTTGTGG CTCTGGGCTG CCCTGCTTGT CCCTGCGGCC 
GCGGTCTACG AAGACCAAGT GGGCAAGTTT GATTGGAGAC AGCAGTATGT TGGGAAGCTC 
AAATTTGCCT CCTTGGAATT TTCCCCTGGA TCCAAGAAGT TGGTTGTGGC CACAGAGAAG 
AATGTGATTG CAGCACTGAA TTCTCGGACT GGGGAGATCT TGTGGCGCCA TGTTGACAAG 
GGCACAGCGG AAGGGGTGGT GGATGCCATG CTGCTCTACG GACAGGATGC AATCACTGTG 
TCCAATGGAG GCCGGATCAT GCGCTCCTGG GAGACAAACA TTGGGGGCCT GAACTGGGAG 
GTTACCCTGG ACAGTCGCAG TCTCCAGGCG CTGGGGCTGG TCGGCCTGCA GGAGGCCGTG 
AGGCACGTTG CGGTCCTGAA GAAGACCACC CTTGCCCTCC TTCACCTCTC CAGCGGGCAC 
CTGAAGTGGG TGGAACACCT CCCGGAGAGT GACAGCATCC ACTACCAGAT GGTGTATTCC 
TACGGCTCGG GGGTCGTGTG GGCCCTCGGA GTCGTCCCCT TCAGCCACGT GAACATTGTC 
AAGTTTAACG TGGAAGATGG AGAGATCGTT CAGCAGGTCA GGGTGTCGAC CCCCTGGCTG 
CGGAGTCTCA CCGGGGCCTG TGGTGTGGTG GACGAGGCTG TCCTGGTGTG CCCCGACCCG 
AGCTCGCGGT CCCTCCAGAC CTTGGCCCTG GAGACGGAGT GGGAGCTGAG GCAGATCCCG 
CTGCAGTCTC TCGACCTGGA ATTCGCAAGT GGATTCCAAC CCCGCGTCCT GCCCACACAG 
CCCAGCCCGG TGGGTCCTTC TCGGGCGCAG TTCTTCCTGC AGTTGTCCCC AAGCCACTAT 
GCGCTGCTGC ACTACCGCCA CGGCGCCCTG GATCTGCTTA AAAACTTCCC GCAGGCTGCC 
CTGGTGAGCT TCGCCACCAC CGGAGAGAAG ACAGTGGCTG CAGTCATGAC CTGTCGGAAC 
GAGGCGAAGC CCAGCGTTTC TGAAGATGGG TCACCTGGGA GCTTTTCGGA GAAGCCTGGC 
CCCCAGGACT CGCTGGCGTG CTTCAACCAG ACCTACACCA TTAACCTCTA CTTGGTGGAG 
ACAGGCCGGC GGCTGCTCGA CACCGCGATC ACCTTTAGCC TGGAACAGAA TGGCACGCGG 
CCCGAGCGGC TGTACATCCA GGTCTTCTTG AAGAAGGATG ACTCCGTGGG CTACCGGGCC 
TTGGTCCAGA CAGAGGATCA CTTGCTGCTT TTCCTGCAGC AGCTGGACGG CCTGCTGGGC 
ATGTTCCTGA AGCGCCTGTC CTCCCAGCTC ATCCTGCTGC AGGCCTGGAC CTCCCACCTC 
TGGAAAGTGT TTTACGATGC GCGGAAGCCC CGGAGCCAGA TCAAGAACGA GATCAACATT 
GACACTCTGG CCAGGGATGA ATTCAACCTC CAGAAGATGA TGGTGATGGT GACGGCCTCG 
GGCAAGCTTT TTGGCATCGA GAGCAGCTCT GGCACCATCC TGTGGAAACA GTATCTCCCG 
AACGTCAAGC CGGACTCCTC CTTTGAGCTG ACGGTCCAGA GGACGACTGC CCACTTCCCC 
CACCCGCCCC AGTGCACGCT CCTGCTGAAA GACAAGGAGA CGGGAATGAG CTCGCTGTAT 
GTCTTCAACC CCATTTTTGG GAAGTGGAGC CAGGTGGCCC CCCCCGTGCT GAAGCGCCCC 
GTCTTGCAGT CTCTGCTCCT GCCCATCATG GATCAGGACT ATGCCAAGGT GCTGTTGCTG 
ATAGACGACG AGTACAAGGT CACAGCTTTC CCAGCCACTC GGAATGTCTT GCGGCAGCTC 
CATGAGCTCG CCCCTTCCAT CTTCTTCTAC TTGGTGGACG CGGATCAGGG GCGGCTGTGC 
GGATATCGAC TCCGGAAGGA CCTCACCACT GAGCTGAGCT GGGAGCTGAC CATCCCCCCG 
GAGGTGCAGC GAATCGTCCA GGTGAAGGGG AAGCGCAGCA GCGAGCATGT CCACTCGCAG 
GGCCGAGTGA TGGGGGACCG CAGCGTGCTC TACAAGAGCC TGAACCCCAA CCTGCTGGCC 
GTGGTGACGG AGAGCACGGA CGTCCACCAC GAGCGCACCT TCATCGGCGT CTTCCTCGTG 
GACGGCGTCA CGGGGCGCAT CGTCCACTCG TCCGTGCAGA GGAAGGCCAA GGGCCCCGTC 
CACATCGTGC ACGCCGAGAA CTGGGTTGCG TACCAGTACT GGAACACCAA GGCTCGGCGC 
AACGAGTTCA CGGCGCTGGA GCTCTACGAG GGCACGGAGC AGTACAATGC CACAGCCTTC 
AGCTCCCTGG ACCGCCCGCA GCTGCCCCAG GTCCTGCAGC AGTCCTACAT CTTCCCCTCC 
GCCATCAGCG CCATGGAGGC CACCATCACA GAGCGGGGCA TCACCAGCCG CCACCTGCTC 
GTTGGGCTGC CTTCTGGAGC GATCCTTTCC CTTCCTAAGG CCTTGCTGGA TCCTCGGCGC 
CCCGAGATCC CAACAGAACA GAGCAGAGAG GAGAACCTGA TCCCGTATTC TCCAGACGTG 
CAGATCCACG CAGAGCGGTT CATCAACTAT AACCAGACAG TCTCCCGAAT GCGAGGCATC 
TACACAGCCC CGTCGGGCCT GGAGTCCACT TGTCTGGTCG TGGCCTACGG TTTGGACATT 
TACCAGACCC GCGTCTACCC GTCCAAACAG TTCGACGTCC TGAAGGACGA CTACGACTAC 
GTGCTCATCA GCAGCGTCCT CTTTGGCCTG GTCTTTGCCA CCATGATCAC GAAGAGGCTG 
GCGCAGGTCA AACTCCTGAA CCGTGCCTGG CGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005203355.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005203412.3 Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

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CloneID	OBa227931
Clone ID Related Accession (Same CDS sequence)	XM_005203355.4
Accession Version	XM_005203355.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3045bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203355.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCCGC GGAGGCGGGG CCCGCGGCGC GGTGCATGCC GGGCCGGCCG GGCCCGCGCT 
CCCATCATGG CGGCGGCCTC GGTTTCCCGG TTGTGGCTCT GGGCTGCCCT GCTTGTCCCT 
GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTTGATT GGAGACAGCA GTATGTTGGG 
AAGCTCAAAT TTGCCTCCTT GGAATTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCCACA 
GAGAAGAATG TGATTGCAGC ACTGAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT 
GACAAGGGCA CAGCGGAAGG GGTGGTGGAT GCCATGCTGC TCTACGGACA GGATGCAATC 
ACTGTGTCCA ATGGAGGCCG GATCATGCGC TCCTGGGAGA CAAACATTGG GGGCCTGAAC 
TGGGAGGTTA CCCTGGACAG TCGCAGTCTC CAGGCGCTGG GGCTGGTCGG CCTGCAGGAG 
GCCGTGAGGC ACGTTGCGGT CCTGAAGAAG ACCACCCTTG CCCTCCTTCA CCTCTCCAGC 
GGGCACCTGA AGTGGGTGGA ACACCTCCCG GAGAGTGACA GCATCCACTA CCAGATGGTG 
TATTCCTACG GCTCGGGGGT CGTGTGGGCC CTCGGAGTCG TCCCCTTCAG CCACGTGAAC 
ATTGTCAAGT TTAACGTGGA AGATGGAGAG ATCGTTCAGC AGGTCAGGGT GTCGACCCCC 
TGGCTGCGGA GTCTCACCGG GGCCTGTGGT GTGGTGGACG AGGCTGTCCT GGTGTGCCCC 
GACCCGAGCT CGCGGTCCCT CCAGACCTTG GCCCTGGAGA CGGAGTGGGA GCTGAGGCAG 
ATCCCGCTGC AGTCTCTCGA CCTGGAATTC GCAAGTGGAT TCCAACCCCG CGTCCTGCCC 
ACACAGCCCA GCCCGGTGGG TCCTTCTCGG GCGCAGTTCT TCCTGCAGTT GTCCCCAAGC 
CACTATGCGC TGCTGCACTA CCGCCACGGC GCCCTGGATC TGCTTAAAAA CTTCCCGCAG 
GCTGCCCTGG TGAGCTTCGC CACCACCGGA GAGAAGACAG TGGCTGCAGT CATGACCTGT 
CGGAACGAGG CGAAGCCCAG CGTTTCTGAA GATGGGTCAC CTGGGAGCTT TTCGGAGAAG 
CCTGGCCCCC AGGACTCGCT GGCGTGCTTC AACCAGACCT ACACCATTAA CCTCTACTTG 
GTGGAGACAG GCCGGCGGCT GCTCGACACC GCGATCACCT TTAGCCTGGA ACAGAATGGC 
ACGCGGCCCG AGCGGCTGTA CATCCAGGTC TTCTTGAAGA AGGATGACTC CGTGGGCTAC 
CGGGCCTTGG TCCAGACAGA GGATCACTTG CTGCTTTTCC TGCAGCAGCT GGCAGGGAGG 
GTGGTGCTGT GGAGCCGCGA GGAGTCCCTG GCCGAGGTGG TGTGCCTGGA GATGGTGGAC 
CTGCCACTGA CCGGGGCACA GGCGGAGCTG GAAGGGGAGT TTGGCAAGAA GGCAGACGGC 
CTGCTGGGCA TGTTCCTGAA GCGCCTGTCC TCCCAGCTCA TCCTGCTGCA GGCCTGGACC 
TCCCACCTCT GGAAAGTGTT TTACGATGCG CGGAAGCCCC GGAGCCAGAT CAAGAACGAG 
ATCAACATTG ACACTCTGGC CAGGGATGAA TTCAACCTCC AGAAGATGAT GGTGATGGTG 
ACGGCCTCGG GCAAGCTTTT TGGCATCGAG AGCAGCTCTG GCACCATCCT GTGGAAACAG 
TATCTCCCGA ACGTCAAGCC GGACTCCTCC TTTGAGCTGA CGGTCCAGAG GACGACTGCC 
CACTTCCCCC ACCCGCCCCA GTGCACGCTC CTGCTGAAAG ACAAGGAGAC GGGAATGAGC 
TCGCTGTATG TCTTCAACCC CATTTTTGGG AAGTGGAGCC AGGTGGCCCC CCCCGTGCTG 
AAGCGCCCCG TCTTGCAGTC TCTGCTCCTG CCCATCATGG ATCAGGACTA TGCCAAGGTG 
CTGTTGCTGA TAGACGACGA GTACAAGGTC ACAGCTTTCC CAGCCACTCG GAATGTCTTG 
CGGCAGCTCC ATGAGCTCGC CCCTTCCATC TTCTTCTACT TGGTGGACGC GGATCAGGGG 
CGGCTGTGCG GATATCGACT CCGGAAGGAC CTCACCACTG AGCTGAGCTG GGAGCTGACC 
ATCCCCCCGG AGGTGCAGCG AATCGTCCAG GTGAAGGGGA AGCGCAGCAG CGAGCATGTC 
CACTCGCAGG GCCGAGTGAT GGGGGACCGC AGCGTGCTCT ACAAGAGCCT GAACCCCAAC 
CTGCTGGCCG TGGTGACGGA GAGCACGGAC GTCCACCACG AGCGCACCTT CATCGGCGTC 
TTCCTCGTGG ACGGCGTCAC GGGGCGCATC GTCCACTCGT CCGTGCAGAG GAAGGCCAAG 
GGCCCCGTCC ACATCGTGCA CGCCGAGAAC TGGGTTGCGT ACCAGTACTG GAACACCAAG 
GCTCGGCGCA ACGAGTTCAC GGCGCTGGAG CTCTACGAGG GCACGGAGCA GTACAATGCC 
ACAGCCTTCA GCTCCCTGGA CCGCCCGCAG CTGCCCCAGG TCCTGCAGCA GTCCTACATC 
TTCCCCTCCG CCATCAGCGC CATGGAGGCC ACCATCACAG AGCGGGGCAT CACCAGCCGC 
CACCTGCTCG TTGGGCTGCC TTCTGGAGCG ATCCTTTCCC TTCCTAAGGC CTTGCTGGAT 
CCTCGGCGCC CCGAGATCCC AACAGAACAG AGCAGAGAGG AGAACCTGAT CCCGTATTCT 
CCAGACGTGC AGATCCACGC AGAGCGGTTC ATCAACTATA ACCAGACAGT CTCCCGAATG 
CGAGGCATCT ACACAGCCCC GTCGGGCCTG GAGTCCACTT GTCTGGTCGT GGCCTACGGT 
TTGGACATTT ACCAGACCCG CGTCTACCCG TCCAAACAGT TCGACGTCCT GAAGGACGAC 
TACGACTACG TGCTCATCAG CAGCGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACG 
AAGAGGCTGG CGCAGGTCAA ACTCCTGAAC CGTGCCTGGC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005203412.3
CDS	26..3070
Translation	MVPRRRGPRRGACRAGRARAPIMAAASVSRLWLWAALLVPAAAVYEDQVGKFDWRQQYVGKLKFASLEFSPGSKKLVVATEKNVIAALNSRTGEILWRHVDKGTAEGVVDAMLLYGQDAITVSNGGRIMRSWETNIGGLNWEVTLDSRSLQALGLVGLQEAVRHVAVLKKTTLALLHLSSGHLKWVEHLPESDSIHYQMVYSYGSGVVWALGVVPFSHVNIVKFNVEDGEIVQQVRVSTPWLRSLTGACGVVDEAVLVCPDPSSRSLQTLALETEWELRQIPLQSLDLEFASGFQPRVLPTQPSPVGPSRAQFFLQLSPSHYALLHYRHGALDLLKNFPQAALVSFATTGEKTVAAVMTCRNEAKPSVSEDGSPGSFSEKPGPQDSLACFNQTYTINLYLVETGRRLLDTAITFSLEQNGTRPERLYIQVFLKKDDSVGYRALVQTEDHLLLFLQQLAGRVVLWSREESLAEVVCLEMVDLPLTGAQAELEGEFGKKADGLLGMFLKRLSSQLILLQAWTSHLWKVFYDARKPRSQIKNEINIDTLARDEFNLQKMMVMVTASGKLFGIESSSGTILWKQYLPNVKPDSSFELTVQRTTAHFPHPPQCTLLLKDKETGMSSLYVFNPIFGKWSQVAPPVLKRPVLQSLLLPIMDQDYAKVLLLIDDEYKVTAFPATRNVLRQLHELAPSIFFYLVDADQGRLCGYRLRKDLTTELSWELTIPPEVQRIVQVKGKRSSEHVHSQGRVMGDRSVLYKSLNPNLLAVVTESTDVHHERTFIGVFLVDGVTGRIVHSSVQRKAKGPVHIVHAENWVAYQYWNTKARRNEFTALELYEGTEQYNATAFSSLDRPQLPQVLQQSYIFPSAISAMEATITERGITSRHLLVGLPSGAILSLPKALLDPRRPEIPTEQSREENLIPYSPDVQIHAERFINYNQTVSRMRGIYTAPSGLESTCLVVAYGLDIYQTRVYPSKQFDVLKDDYDYVLISSVLFGLVFATMITKRLAQVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005203355.4
Organism	Bos taurus(cattle)
Definition	Bos taurus ER membrane protein complex subunit 1 (EMC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005203355.4

ORF Insert Sequence:

ATGGTTCCGC GGAGGCGGGG CCCGCGGCGC GGTGCATGCC GGGCCGGCCG GGCCCGCGCT 
CCCATCATGG CGGCGGCCTC GGTTTCCCGG TTGTGGCTCT GGGCTGCCCT GCTTGTCCCT 
GCGGCCGCGG TCTACGAAGA CCAAGTGGGC AAGTTTGATT GGAGACAGCA GTATGTTGGG 
AAGCTCAAAT TTGCCTCCTT GGAATTTTCC CCTGGATCCA AGAAGTTGGT TGTGGCCACA 
GAGAAGAATG TGATTGCAGC ACTGAATTCT CGGACTGGGG AGATCTTGTG GCGCCATGTT 
GACAAGGGCA CAGCGGAAGG GGTGGTGGAT GCCATGCTGC TCTACGGACA GGATGCAATC 
ACTGTGTCCA ATGGAGGCCG GATCATGCGC TCCTGGGAGA CAAACATTGG GGGCCTGAAC 
TGGGAGGTTA CCCTGGACAG TCGCAGTCTC CAGGCGCTGG GGCTGGTCGG CCTGCAGGAG 
GCCGTGAGGC ACGTTGCGGT CCTGAAGAAG ACCACCCTTG CCCTCCTTCA CCTCTCCAGC 
GGGCACCTGA AGTGGGTGGA ACACCTCCCG GAGAGTGACA GCATCCACTA CCAGATGGTG 
TATTCCTACG GCTCGGGGGT CGTGTGGGCC CTCGGAGTCG TCCCCTTCAG CCACGTGAAC 
ATTGTCAAGT TTAACGTGGA AGATGGAGAG ATCGTTCAGC AGGTCAGGGT GTCGACCCCC 
TGGCTGCGGA GTCTCACCGG GGCCTGTGGT GTGGTGGACG AGGCTGTCCT GGTGTGCCCC 
GACCCGAGCT CGCGGTCCCT CCAGACCTTG GCCCTGGAGA CGGAGTGGGA GCTGAGGCAG 
ATCCCGCTGC AGTCTCTCGA CCTGGAATTC GCAAGTGGAT TCCAACCCCG CGTCCTGCCC 
ACACAGCCCA GCCCGGTGGG TCCTTCTCGG GCGCAGTTCT TCCTGCAGTT GTCCCCAAGC 
CACTATGCGC TGCTGCACTA CCGCCACGGC GCCCTGGATC TGCTTAAAAA CTTCCCGCAG 
GCTGCCCTGG TGAGCTTCGC CACCACCGGA GAGAAGACAG TGGCTGCAGT CATGACCTGT 
CGGAACGAGG CGAAGCCCAG CGTTTCTGAA GATGGGTCAC CTGGGAGCTT TTCGGAGAAG 
CCTGGCCCCC AGGACTCGCT GGCGTGCTTC AACCAGACCT ACACCATTAA CCTCTACTTG 
GTGGAGACAG GCCGGCGGCT GCTCGACACC GCGATCACCT TTAGCCTGGA ACAGAATGGC 
ACGCGGCCCG AGCGGCTGTA CATCCAGGTC TTCTTGAAGA AGGATGACTC CGTGGGCTAC 
CGGGCCTTGG TCCAGACAGA GGATCACTTG CTGCTTTTCC TGCAGCAGCT GGCAGGGAGG 
GTGGTGCTGT GGAGCCGCGA GGAGTCCCTG GCCGAGGTGG TGTGCCTGGA GATGGTGGAC 
CTGCCACTGA CCGGGGCACA GGCGGAGCTG GAAGGGGAGT TTGGCAAGAA GGCAGACGGC 
CTGCTGGGCA TGTTCCTGAA GCGCCTGTCC TCCCAGCTCA TCCTGCTGCA GGCCTGGACC 
TCCCACCTCT GGAAAGTGTT TTACGATGCG CGGAAGCCCC GGAGCCAGAT CAAGAACGAG 
ATCAACATTG ACACTCTGGC CAGGGATGAA TTCAACCTCC AGAAGATGAT GGTGATGGTG 
ACGGCCTCGG GCAAGCTTTT TGGCATCGAG AGCAGCTCTG GCACCATCCT GTGGAAACAG 
TATCTCCCGA ACGTCAAGCC GGACTCCTCC TTTGAGCTGA CGGTCCAGAG GACGACTGCC 
CACTTCCCCC ACCCGCCCCA GTGCACGCTC CTGCTGAAAG ACAAGGAGAC GGGAATGAGC 
TCGCTGTATG TCTTCAACCC CATTTTTGGG AAGTGGAGCC AGGTGGCCCC CCCCGTGCTG 
AAGCGCCCCG TCTTGCAGTC TCTGCTCCTG CCCATCATGG ATCAGGACTA TGCCAAGGTG 
CTGTTGCTGA TAGACGACGA GTACAAGGTC ACAGCTTTCC CAGCCACTCG GAATGTCTTG 
CGGCAGCTCC ATGAGCTCGC CCCTTCCATC TTCTTCTACT TGGTGGACGC GGATCAGGGG 
CGGCTGTGCG GATATCGACT CCGGAAGGAC CTCACCACTG AGCTGAGCTG GGAGCTGACC 
ATCCCCCCGG AGGTGCAGCG AATCGTCCAG GTGAAGGGGA AGCGCAGCAG CGAGCATGTC 
CACTCGCAGG GCCGAGTGAT GGGGGACCGC AGCGTGCTCT ACAAGAGCCT GAACCCCAAC 
CTGCTGGCCG TGGTGACGGA GAGCACGGAC GTCCACCACG AGCGCACCTT CATCGGCGTC 
TTCCTCGTGG ACGGCGTCAC GGGGCGCATC GTCCACTCGT CCGTGCAGAG GAAGGCCAAG 
GGCCCCGTCC ACATCGTGCA CGCCGAGAAC TGGGTTGCGT ACCAGTACTG GAACACCAAG 
GCTCGGCGCA ACGAGTTCAC GGCGCTGGAG CTCTACGAGG GCACGGAGCA GTACAATGCC 
ACAGCCTTCA GCTCCCTGGA CCGCCCGCAG CTGCCCCAGG TCCTGCAGCA GTCCTACATC 
TTCCCCTCCG CCATCAGCGC CATGGAGGCC ACCATCACAG AGCGGGGCAT CACCAGCCGC 
CACCTGCTCG TTGGGCTGCC TTCTGGAGCG ATCCTTTCCC TTCCTAAGGC CTTGCTGGAT 
CCTCGGCGCC CCGAGATCCC AACAGAACAG AGCAGAGAGG AGAACCTGAT CCCGTATTCT 
CCAGACGTGC AGATCCACGC AGAGCGGTTC ATCAACTATA ACCAGACAGT CTCCCGAATG 
CGAGGCATCT ACACAGCCCC GTCGGGCCTG GAGTCCACTT GTCTGGTCGT GGCCTACGGT 
TTGGACATTT ACCAGACCCG CGTCTACCCG TCCAAACAGT TCGACGTCCT GAAGGACGAC 
TACGACTACG TGCTCATCAG CAGCGTCCTC TTTGGCCTGG TCTTTGCCAC CATGATCACG 
AAGAGGCTGG CGCAGGTCAA ACTCCTGAAC CGTGCCTGGC GGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EMC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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