ITCH cDNA ORF clone, Bos taurus(cattle)

The following ITCH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITCH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa36501 NM_001082428.2
Latest version!
Bos taurus itchy E3 ubiquitin protein ligase (ITCH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa47141 XM_010811429.3
Latest version!
Bos taurus itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
View Old Accession Versions >>
OBa47141 XM_010811429.2 Bos taurus itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa36501
    Clone ID Related Accession (Same CDS sequence) NM_001082428.2
    Accession Version NM_001082428.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-30
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Itchy homolog
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133503.1. On Aug 31, 2012 this sequence version replaced NM_001082428.1. ##Evidence-Data-START## Transcript exon combination :: BC133503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGGAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001075897.1
    CDS139..2727
    Translation

    Target ORF information:

    RefSeq Version NM_001082428.2
    Organism Bos taurus(cattle)
    Definition Bos taurus itchy E3 ubiquitin protein ligase (ITCH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001082428.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGGAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47141
    Clone ID Related Accession (Same CDS sequence) XM_010811429.2 , XM_010811429.3
    Accession Version XM_010811429.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Itchy homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809731.1
    CDS336..2924
    Translation

    Target ORF information:

    RefSeq Version XM_010811429.2
    Organism Bos taurus(cattle)
    Definition Bos taurus itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811429.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa47141
    Clone ID Related Accession (Same CDS sequence) XM_010811429.2 , XM_010811429.3
    Accession Version XM_010811429.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase Itchy homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037340.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811429.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010809731.1
    CDS330..2918
    Translation

    Target ORF information:

    RefSeq Version XM_010811429.3
    Organism Bos taurus(cattle)
    Definition Bos taurus itchy E3 ubiquitin protein ligase (ITCH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811429.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTCTGACA GTGGATCACA GCTTGGTTCA ATGGGTAGTT TGACCATGAA ATCACAACTT 
    CAGATCACTG TCATTTCAGC AAAACTTAAA GAAAATAAAA AGAATTGGTT TGGGCCAAGT
    CCTTATGTGG AAGTCACAGT AGATGGACAG TCAAAGAAGA CAGAAAAATG CAACAATACG
    AACAGTCCCA AATGGAAGCA GCCTCTTACA GTTATTGTCA CCCCTGTGAG TAAATTACAT
    TTTCGTGTGT GGAGTCACCA GACACTGAAA TCTGATGTTT TGTTGGGAAC TGCTGGATTG
    GATATCTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TGTGACTTTA
    CAGCTTGTAG GTGACAAAGA ACCAGCAGAG ACAATAGGAG ATTTGTCAGT CTGTCTTGAT
    GGGCTGCAAC TAGAGTCTGA AGTTGTTGCC AATGGTGAAA CTGCATGTTC AGAAAGTGCT
    TCACACAATG ATGATGGCTC CAGGCCCAAG GATGAAACAA GAGCGAACGC AAGTGGATCA
    GATGACCCTG AGGATGCAGG GTCTGGTGAA AATAGGAGGA TCAATGGGAA TAATTCTCCA
    TCACTCTCAA ATGGCGGTTT TAAACCTTCT AGGCCTCCAA GACCTTCACG ACCACCTCCA
    CCTACCCCAC GTAGACCAGC ATCTGTCAAT GGCTCACCAT CTACCACTTC TGAAAGTGAT
    GGCTCTAGTA CAGGCTCTCT GCCACCAACA AATACAAATT CAAGTACATC TGAAGGAGCA
    ACATCTGGAT TAATAATTCC TCTTACTATA TCTGGAGGTG CAGGCCCTAG ACCATTAAAT
    CCTATACCTC AAGCTCCCCT CCCACCTGGT TGGGAGCAGA GAGTGGACCA GCATGGGCGA
    GTTTACTATG TAGATCATAT TGAAAAAAGA ACAACGTGGG ATAGACCAGA ACCTCTACCT
    CCTGGCTGGG AGCGGCGGGT TGACAACATG GGACGTATTT ATTATGTTGA CCATTTCACA
    AGAACAACAA CGTGGCAGAG GCCAACACTG GAATCAGTCC GGAACTATGA ACAATGGCAG
    CTCCAGCGTA GTCAACTTCA GGGAGCTATG CAGCAGTTTA ACCAGAGGTT CATTTATGGG
    AATCAAGATT TGTTTGCTAC ATCACAAAAT AAAGAATTTG ATCCCCTGGG TCCCCTGCCG
    CCTGGTTGGG AGAAGAGAAC GGACAGCAAT GGTAGAGTAT ATTTTGTCAA CCACAACACT
    CGTATTACAC AATGGGAAGA CCCCAGAAAT CAAGGTCAAT TAAATGAAAA GCCCTTACCT
    GAAGGCTGGG AAATGAGATT CACGGTAGAC GGAATTCCGT ATTTTGTGGA CCACAATAGA
    AGAACAACCA CCTATATAGA TCCCCGCACC GGAAAATCTG CCTTAGACAA TGGACCCCAG
    ATAGCCTATG TACGGGACTT CAAGGCAAAG GTTCAATATT TCCGTTTCTG GTGTCAGCAA
    CTGGCCATGC CACAGCACAT AAAGATTACA GTGACAAGAA AAACATTGTT TGAGGATTCC
    TTTCAGCAGA TAATGAGCTT CAGTCCTCAA GATCTGCGAA GACGTTTGTG GGTGATTTTT
    CCAGGAGAAG AAGGTTTAGA TTATGGAGGT GTAGCAAGAG AATGGTTCTT TCTTTTGTCA
    CATGAGGTGT TGAACCCAAT GTATTGCCTG TTTGAATATG CAGGGAAGGA TAACTACTGC
    TTGCAGATAA ACCCCGCTTC TTACATCAAT CCAGATCACC TGAAATATTT TCGTTTTATT
    GGCAGGTTTA TTGCTATGGC TCTGTTCCAT GGGAAATTCA TAGACACAGG TTTTTCTTTG
    CCATTCTATA AGCGTATCTT GAATAAACCA GTTGGACTGA AGGATTTAGA ATCGATTGAT
    CCAGAATTTT ACAATTCTCT CATCTGGGTT AAAGAGAACA ATATTGAGGA GTGTGGTTTG
    GAAATGTATT TCTCCGTTGA TAAAGAAATC TTAGGTGAAA TCAAGAGTCA TGATTTGAAA
    CCCAATGGTG GCAATATTCT TGTAACAGAA GAAAATAAAG AGGAATATAT CAGAATGGTA
    GCTGAATGGA GATTGTCTCG TGGTGTTGAA GAACAGACGC AAGCTTTCTT TGAGGGCTTT
    AATGAAATTC TTCCCCAGCA GTATTTACAG TACTTCGATG CAAAGGAATT AGAGGTTCTT
    TTATGTGGGA TGCAAGAGAT TGATTTGAAT GATTGGCAAA GACATGCCAT CTACCGTCAC
    TATACCAGGA CAAGTAAACA AATCATGTGG TTTTGGCAGT TTGTTAAAGA AATCGATAAT
    GAGAAGAGAA TGCGACTTTT GCAGTTTGTT ACTGGGACCT GCCGACTGCC AGTTGGAGGA
    TTTGCTGACC TCATGGGGAG CAATGGGCCA CAGAAGTTCT GCATTGAAAA AGTCGGGAAA
    GAAAATTGGT TGCCTAGAAG TCATACCTGT TTTAACCGCC TGGACCTGCC ACCCTACAAG
    AGCTATGAGC AACTGAAGGA AAAGCTGTTA TTTGCTATTG AAGAAACAGA AGGCTTTGGA
    CAAGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.