MICAL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MICAL1
Entrez Gene ID	508306
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 1
Synonyms	MICAL-1
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 1 Names [F-actin]-monooxygenase MICAL1 protein-methionine sulfoxide oxidase MICAL1 [F-actin]-methionine sulfoxide oxidase MICAL1 microtubule associated monoxygenase, calponin and LIM domain containing 1 molecule interacting with CasL protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010808425.2	XP_010806727.1	protein-methionine sulfoxide oxidase MICAL1 isoform X1
XM_024996479.1	XP_024852247.1	F-actin-monooxygenase MICAL1 isoform X1
XM_005210774.3	XP_005210831.1	protein-methionine sulfoxide oxidase MICAL1 isoform X2
XM_024996480.1	XP_024852248.1	F-actin-monooxygenase MICAL1 isoform X1
XM_005210773.3	XP_005210830.1	protein-methionine sulfoxide oxidase MICAL1 isoform X1
XM_024996481.1	XP_024852249.1	F-actin-monooxygenase MICAL1 isoform X1
NM_001081582.1	NP_001075051.1	[F-actin]-monooxygenase MICAL1

Pathways from BioSystems

Homologies

	MICAL1	XP_001090339.1
Homo sapiens (human)	MICAL1	NP_001273542.1
Pan troglodytes (chimpanzee)	MICAL1	XP_001153132.2
Rattus norvegicus (Norway rat)	Mical1	NP_001099867.1
Canis lupus familiaris (dog)	MICAL1	XP_005627842.1
Mus musculus (house mouse)	Mical1	NP_612188.1
Gallus gallus (chicken)	MICAL1	NP_001026747.1
Bos taurus (cattle)	MICAL1	NP_001075051.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MICAL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MICAL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa22551	XM_005210774.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OBa237683	XM_024996480.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa237683	XM_024996481.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa237683	XM_024996479.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa42340	NM_001081582.1 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa22550	XM_010808425.2 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa22550	XM_005210773.3 Latest version!	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MICAL1 ( XM_005210774.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210831.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa22551
Clone ID Related Accession (Same CDS sequence)	XM_005210774.3
Accession Version	XM_005210774.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104190.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210774.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTGAGTTCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210831.1
CDS	389..3292
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSEFP

Target ORF information:

RefSeq Version	XM_005210774.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210774.3

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTGAGTTCCC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_024996479.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852247.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa237683
Clone ID Related Accession (Same CDS sequence)	XM_024996479.1 , XM_024996480.1 , XM_024996481.1
Accession Version	XM_024996479.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852247.1
CDS	408..3632
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQGLLQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	XM_024996479.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996479.1

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_024996480.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852248.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa237683
Clone ID Related Accession (Same CDS sequence)	XM_024996479.1 , XM_024996480.1 , XM_024996481.1
Accession Version	XM_024996480.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852248.1
CDS	211..3435
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQGLLQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	XM_024996480.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996480.1

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_024996481.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024852249.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa237683
Clone ID Related Accession (Same CDS sequence)	XM_024996479.1 , XM_024996480.1 , XM_024996481.1
Accession Version	XM_024996481.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3225bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	F-actin-monooxygenase MICAL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037336.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024852249.1
CDS	381..3605
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQGLLQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	XM_024996481.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024996481.1

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGGCTTG TTGCAGGAAA ATGGGGAGGA TGCTGGTGGC 
AAGAAGCCTC GCCTGGAGGT AAAGGCGGAG ACCCCAAGTA CTGAGGAGCC ACCTGTCCCA 
AAGCCTGATG AACCAATGAC ACCACCATCC CAGCAGCAGG ATGCCAGTGC TGAGGATCTG 
TGTGCACTTT GTGGGCAACA CCTCTATATC CTGGAACGCC TCTGTGCTGA TGGCCGTTTC 
TTCCACCGGA GCTGCTTCCG CTGTCATATC TGTGAGGCCA CATTATGGCC AGGTGGCTAT 
AGGCAGCACC CAGGAGATGG ATATTTATAC TGCCTCCAGC ACCTGCCTCA GACAGGCCAC 
GAAGAAGATA GCAGCGATAG AGGTCCTGAG AGTCAGGACC TTCCCATGTC GAGTGAGAAT 
AACACGCCAT CAGGCCCCGC AACTCCCGTG GACCTCCATC AAGGGACCAG TCCTGTCCCA 
AACCCCATCC AGCCCACCCG TCGATTGATC CGCCTCTCCA GCCCAGAACG CCAGCGTTTA 
TCCTCCCTTC ATCTCACCCC TGACCCGGAA ATGGAGCCGC CACCCAAGCC CCCCCGAAGC 
TGCTCCACTT TGGCCCACCA AGCCCTGGAA GCAAGCTTCA AGGGCTGGGG AATGCCAGTC 
CAGAGCCCTC AAGTTCTTGA GGCCATGGAA ATGGGGGAAG AAGAGAGGTC CTCCTCCAGT 
GAAGAGGAAA CAGAGGAAGA GGAAGATGTG CCTTTGGACT CAGACATGGA ACATTTTCTG 
AGGAACTTGG CTGAGAACTC AGGCACCATG AACAATTATC CAACGTGGCG TCGGACTCTG 
CTGCGCCGGG CCAAGGAGGA GGAGATGAAG CGGTTCTGCA AGGCTCAGGC CATCCAGCGG 
CGACTAAATG AGATCGAGGC TGCTCTAAGG GAGCTGGAGG CCAGGGGCAC AGAGCTGGAG 
CTGGCTTTGA GGAGCCAAAG CAGTTCCCCT GAAAAGCAAA AGGCATTATG GGTGGAACAG 
CTGCTACAGC TCGTCCAGAA GAAAAACAGC CTAGTGGCCG AGGAGGCTGA GCTCATGATC 
ACAGTGCAGG AGCTGAACTT GGAGGAGAAA CAGTGGCAGC TGGACCAAGA GCTGCGAACC 
TACATGAACC GGGAAGAAAC CCTAAAGACA GCTGCCGATC GGCAGGCTGA GGACCAGGTC 
CTGAGGAAGC TACTGGATGT GGTGAACCAG CGAGATGCTC TCATCCGCCT CCAGGAGGAG 
CGCAGGCTCA GTGAGCTGGC CTCGGAGCCC GGGGTCCAGG GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( NM_001081582.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001075051.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), mRNA.

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CloneID	OBa42340
Clone ID Related Accession (Same CDS sequence)	NM_001081582.1
Accession Version	NM_001081582.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	[F-actin]-monooxygenase MICAL1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133298.1. On Feb 20, 2007 this sequence version replaced XM_864490.2. ##Evidence-Data-START## Transcript exon combination :: BC133298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGGCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001075051.1
CDS	227..3439
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPGLLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	NM_001081582.1
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001081582.1

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGGCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_010808425.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806727.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa22550
Clone ID Related Accession (Same CDS sequence)	XM_010808425.2 , XM_005210773.3
Accession Version	XM_010808425.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104190.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010808425.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806727.1
CDS	359..3571
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	XM_010808425.2
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010808425.2

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MICAL1 ( XM_005210773.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005210830.1 Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa22550
Clone ID Related Accession (Same CDS sequence)	XM_010808425.2 , XM_005210773.3
Accession Version	XM_005210773.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	protein-methionine sulfoxide oxidase MICAL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104190.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005210773.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005210830.1
CDS	233..3445
Translation	MASTISTNPAHAHFESFLQAQLCQDVLSSFQGLCGALGVEPGGGLSQYHKVKAQLNYWNAKSLWAKLDKRASQPVYQQGRACTGTKCLVVGAGPCGLRAAVELAMLGARVVLVEKRTKFSRHNVLHLWPFTIHDLRALGAKKFYGRFCTGSLDHISIRQLQLLLLKVALLLGVEIHWGITFTGLQPPPKKGSGWRAQLQPSPPAQLAKYEFDVLISAAGGKFVPEGFTVREMRGKLAIGITANFVNGRTVEETQVPEISGVARIYNQSFFQSLLKATGIDLENIVYYKDDTHYFVMTAKKQCLLRLGVLHKDWPDTERLLGSANVVPEALQRFARAAADFATHGKLGKLEFARDAHGRPDVSAFDFTSMMRAESSARVQERHGTRLLLGLVGDCLVEPFWPLGTGVARGFLAAFDAAWMVKRWAEGAGPLEVLAERESLYQLLSQTSPENMHRNVAQYGLDPATRYPNLNLRAVTPSQVRDLYDMEAKEPVQRMSDETDSGKAATGAVGSQEELLRWCQEQTAGYPGVHVTDLSSSWADGLALCALVHRLRPALLEPSELQGMGALEATSWALKMAEHELGITPVLSAQAMVAGSDPLGLIAYLSHFHSAFKSVPHNPGSVSQGSPGTASAVLFLGKLQRTLQRTRTQENGEDAGGKKPRLEVKAETPSTEEPPVPKPDEPMTPPSQQQDASAEDLCALCGQHLYILERLCADGRFFHRSCFRCHICEATLWPGGYRQHPGDGYLYCLQHLPQTGHEEDSSDRGPESQDLPMSSENNTPSGPATPVDLHQGTSPVPNPIQPTRRLIRLSSPERQRLSSLHLTPDPEMEPPPKPPRSCSTLAHQALEASFKGWGMPVQSPQVLEAMEMGEEERSSSSEEETEEEEDVPLDSDMEHFLRNLAENSGTMNNYPTWRRTLLRRAKEEEMKRFCKAQAIQRRLNEIEAALRELEARGTELELALRSQSSSPEKQKALWVEQLLQLVQKKNSLVAEEAELMITVQELNLEEKQWQLDQELRTYMNREETLKTAADRQAEDQVLRKLLDVVNQRDALIRLQEERRLSELASEPGVQG

Target ORF information:

RefSeq Version	XM_005210773.3
Organism	Bos taurus(cattle)
Definition	Bos taurus microtubule associated monooxygenase, calponin and LIM domain containing 1 (MICAL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005210773.3

ORF Insert Sequence:

ATGGCCTCAA CCATCTCCAC CAACCCAGCG CATGCCCACT TTGAGAGCTT CTTGCAGGCC 
CAGCTGTGCC AGGATGTGTT GAGCAGCTTT CAAGGGCTAT GCGGGGCCCT GGGGGTGGAG 
CCTGGTGGGG GGCTCTCCCA GTACCACAAG GTCAAGGCCC AGCTCAACTA CTGGAATGCC 
AAGTCGCTGT GGGCCAAGCT GGACAAGAGA GCGAGCCAGC CGGTCTACCA GCAGGGCCGG 
GCCTGCACCG GCACTAAGTG CCTGGTGGTG GGTGCGGGAC CTTGCGGTCT GCGGGCTGCT 
GTGGAGCTGG CGATGCTGGG GGCCCGAGTG GTGCTGGTGG AAAAGCGCAC CAAGTTCTCT 
CGCCACAACG TGCTCCACCT CTGGCCCTTC ACCATCCACG ACCTTCGGGC ACTCGGCGCC 
AAGAAGTTCT ATGGGCGCTT CTGCACAGGC TCCCTGGACC ACATCAGCAT CCGGCAACTG 
CAGCTCCTCT TGTTGAAAGT GGCATTACTG CTGGGGGTGG AAATCCACTG GGGCATCACT 
TTCACTGGCC TGCAGCCTCC TCCCAAAAAG GGGAGTGGTT GGCGTGCCCA GCTCCAGCCC 
AGCCCCCCAG CCCAACTGGC CAAGTATGAA TTTGATGTCC TCATCTCTGC GGCTGGAGGT 
AAATTCGTCC CTGAAGGCTT CACAGTGCGA GAAATGCGCG GCAAACTGGC AATTGGCATC 
ACGGCTAACT TTGTGAACGG GCGCACCGTG GAAGAGACAC AGGTGCCCGA GATCAGTGGT 
GTGGCCAGGA TCTACAACCA GAGCTTCTTC CAGAGCCTGC TCAAAGCTAC AGGCATCGAT 
CTGGAGAATA TCGTGTACTA CAAAGATGAC ACCCACTACT TTGTGATGAC AGCCAAGAAG 
CAGTGCCTGC TACGGCTGGG AGTGCTGCAT AAGGACTGGC CCGATACTGA ACGGCTGCTG 
GGCAGCGCCA ATGTGGTGCC CGAGGCTTTG CAGCGCTTTG CTCGGGCAGC TGCCGACTTC 
GCCACCCACG GCAAACTTGG GAAGCTGGAG TTTGCCCGGG ACGCCCACGG GCGGCCGGAC 
GTCTCTGCCT TTGACTTCAC AAGTATGATG CGGGCAGAGA GCTCTGCTCG GGTGCAGGAG 
AGGCACGGCA CCCGCCTGCT GCTGGGGCTG GTCGGGGACT GCCTGGTGGA GCCCTTCTGG 
CCCCTGGGCA CTGGAGTGGC CCGGGGCTTC CTGGCAGCCT TTGATGCCGC CTGGATGGTG 
AAGCGGTGGG CAGAGGGCGC TGGGCCCCTA GAGGTGTTGG CAGAGAGAGA GAGCTTGTAC 
CAGCTCCTGT CACAGACGTC CCCGGAGAAC ATGCACCGCA ACGTGGCACA GTACGGGCTG 
GACCCCGCCA CCCGCTACCC CAACCTGAAC CTCCGGGCTG TGACCCCCAG TCAGGTACGA 
GACCTATACG ACATGGAGGC CAAGGAGCCT GTGCAGAGGA TGAGTGACGA GACAGACTCC 
GGAAAGGCAG CCACTGGGGC AGTGGGCTCC CAGGAGGAGC TGCTGCGCTG GTGCCAGGAG 
CAGACGGCTG GATACCCAGG TGTCCATGTC ACTGACCTGT CTTCCTCCTG GGCTGATGGG 
CTGGCTCTGT GTGCCCTGGT GCACCGGCTG CGGCCCGCCC TACTGGAACC CTCCGAGCTC 
CAGGGAATGG GGGCTCTGGA AGCTACTTCT TGGGCGCTGA AGATGGCAGA GCATGAGCTG 
GGCATCACAC CAGTGTTGTC CGCACAGGCG ATGGTGGCAG GGAGTGACCC ACTTGGCCTC 
ATTGCCTACC TCAGCCACTT CCATAGTGCC TTCAAGAGCG TACCACACAA CCCAGGCTCG 
GTCAGCCAAG GCTCCCCGGG CACTGCCAGC GCTGTACTAT TCCTTGGCAA ACTGCAGAGG 
ACCCTGCAAC GGACCCGAAC TCAGGAAAAT GGGGAGGATG CTGGTGGCAA GAAGCCTCGC 
CTGGAGGTAA AGGCGGAGAC CCCAAGTACT GAGGAGCCAC CTGTCCCAAA GCCTGATGAA 
CCAATGACAC CACCATCCCA GCAGCAGGAT GCCAGTGCTG AGGATCTGTG TGCACTTTGT 
GGGCAACACC TCTATATCCT GGAACGCCTC TGTGCTGATG GCCGTTTCTT CCACCGGAGC 
TGCTTCCGCT GTCATATCTG TGAGGCCACA TTATGGCCAG GTGGCTATAG GCAGCACCCA 
GGAGATGGAT ATTTATACTG CCTCCAGCAC CTGCCTCAGA CAGGCCACGA AGAAGATAGC 
AGCGATAGAG GTCCTGAGAG TCAGGACCTT CCCATGTCGA GTGAGAATAA CACGCCATCA 
GGCCCCGCAA CTCCCGTGGA CCTCCATCAA GGGACCAGTC CTGTCCCAAA CCCCATCCAG 
CCCACCCGTC GATTGATCCG CCTCTCCAGC CCAGAACGCC AGCGTTTATC CTCCCTTCAT 
CTCACCCCTG ACCCGGAAAT GGAGCCGCCA CCCAAGCCCC CCCGAAGCTG CTCCACTTTG 
GCCCACCAAG CCCTGGAAGC AAGCTTCAAG GGCTGGGGAA TGCCAGTCCA GAGCCCTCAA 
GTTCTTGAGG CCATGGAAAT GGGGGAAGAA GAGAGGTCCT CCTCCAGTGA AGAGGAAACA 
GAGGAAGAGG AAGATGTGCC TTTGGACTCA GACATGGAAC ATTTTCTGAG GAACTTGGCT 
GAGAACTCAG GCACCATGAA CAATTATCCA ACGTGGCGTC GGACTCTGCT GCGCCGGGCC 
AAGGAGGAGG AGATGAAGCG GTTCTGCAAG GCTCAGGCCA TCCAGCGGCG ACTAAATGAG 
ATCGAGGCTG CTCTAAGGGA GCTGGAGGCC AGGGGCACAG AGCTGGAGCT GGCTTTGAGG 
AGCCAAAGCA GTTCCCCTGA AAAGCAAAAG GCATTATGGG TGGAACAGCT GCTACAGCTC 
GTCCAGAAGA AAAACAGCCT AGTGGCCGAG GAGGCTGAGC TCATGATCAC AGTGCAGGAG 
CTGAACTTGG AGGAGAAACA GTGGCAGCTG GACCAAGAGC TGCGAACCTA CATGAACCGG 
GAAGAAACCC TAAAGACAGC TGCCGATCGG CAGGCTGAGG ACCAGGTCCT GAGGAAGCTA 
CTGGATGTGG TGAACCAGCG AGATGCTCTC ATCCGCCTCC AGGAGGAGCG CAGGCTCAGT 
GAGCTGGCCT CGGAGCCCGG GGTCCAGGGC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MICAL1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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