TRPM2 cDNA ORF clone, Bos taurus(cattle)

The following TRPM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa219299 XM_024996770.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OBa16114 XM_024996742.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa219297 XM_024996744.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219298 XM_024996748.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219298 XM_024996758.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219298 XM_024996764.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa219298 XM_024996756.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa87836 XM_015473833.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa87836 XM_015462179.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa87834 XM_015462177.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015462176.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015473824.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015473825.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015462173.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015473822.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015473826.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015462175.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015462174.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87834 XM_015473828.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87835 XM_015473831.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa87835 XM_015462178.1
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OBa16113 XM_024996733.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16113 XM_024996739.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16113 XM_010824475.2
Latest version!
Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16113 XM_024996729.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16113 XM_024996726.1
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa16113 XM_010801661.2
Latest version!
Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa16113 XM_010801661.1 Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa219299
    Clone ID Related Accession (Same CDS sequence) XM_024996770.1
    Accession Version XM_024996770.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTACAACC CGCCCTTCTA CACGGCCAAC AGGAAGGACA AGGACCTGGT GGACCCCGTG 
    GGAGATGCCC TGGAACCTCT GTCCAGGATC AGCTACAATG CAGTGGACGG ACCACTGGAC
    CGGCGCAGCT TCCATGGAGT CTACACAGTG CGGGACGGGC TCCCTCTAAA CCCCATGGGT
    CGCACGGGAC TGCGCGGCCG CGGGGACCTC AGCCGCTTCG GCCCCAATCA CACACTGCAG
    CCTGTGATCA CCCGCTGGAG GCGGAGCCTG GATGGTGCCA TCTGCAGGAA GAACGTCAAG
    AAGATGCTGG AGGTGCTGGT GGTGAAGCAT GGCCCCTCCG AGCACTGGAT GCTGCCTGGG
    GGCTCCCGGG AGCCAGGAGA GTTGCTGCCC CAGAATTTAA AACAGGTCCT GCAAAGAGAG
    TTCTGGTCTT CCTTCGAGCG CCTGCTGACT CAGGGCATGA AGGTGTACAA AGGATACATG
    GATGACCCCA GGAACACGGA CAATGCCTGG ATCGAGACGG TGGCTGTGAG CATCCACTTC
    CCAGACCAGA GCGATGTGGA GCTCAAGAGG CTGAACTCTC ATCTGCACTC CTGTGACGAG
    GGGATGGCCA TTCGCTGGCA GGTGGTGGAT GAGCGCATCC CCCTGTATGA CAACCACAAG
    GTCATCCTCC AGAAAGTGGC CGCCTTGTTC ATGGCCTACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852538.1
    CDS222..926
    Translation

    Target ORF information:

    RefSeq Version XM_024996770.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996770.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGTACAACC CGCCCTTCTA CACGGCCAAC AGGAAGGACA AGGACCTGGT GGACCCCGTG 
    GGAGATGCCC TGGAACCTCT GTCCAGGATC AGCTACAATG CAGTGGACGG ACCACTGGAC
    CGGCGCAGCT TCCATGGAGT CTACACAGTG CGGGACGGGC TCCCTCTAAA CCCCATGGGT
    CGCACGGGAC TGCGCGGCCG CGGGGACCTC AGCCGCTTCG GCCCCAATCA CACACTGCAG
    CCTGTGATCA CCCGCTGGAG GCGGAGCCTG GATGGTGCCA TCTGCAGGAA GAACGTCAAG
    AAGATGCTGG AGGTGCTGGT GGTGAAGCAT GGCCCCTCCG AGCACTGGAT GCTGCCTGGG
    GGCTCCCGGG AGCCAGGAGA GTTGCTGCCC CAGAATTTAA AACAGGTCCT GCAAAGAGAG
    TTCTGGTCTT CCTTCGAGCG CCTGCTGACT CAGGGCATGA AGGTGTACAA AGGATACATG
    GATGACCCCA GGAACACGGA CAATGCCTGG ATCGAGACGG TGGCTGTGAG CATCCACTTC
    CCAGACCAGA GCGATGTGGA GCTCAAGAGG CTGAACTCTC ATCTGCACTC CTGTGACGAG
    GGGATGGCCA TTCGCTGGCA GGTGGTGGAT GAGCGCATCC CCCTGTATGA CAACCACAAG
    GTCATCCTCC AGAAAGTGGC CGCCTTGTTC ATGGCCTACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16114
    Clone ID Related Accession (Same CDS sequence) XM_024996742.1
    Accession Version XM_024996742.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852510.1
    CDS1737..6218
    Translation

    Target ORF information:

    RefSeq Version XM_024996742.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996742.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219297
    Clone ID Related Accession (Same CDS sequence) XM_024996744.1
    Accession Version XM_024996744.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4398bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGTGTA CAAAGGATAC ATGGATGACC CCAGGAACAC GGACAATGCC
    TGGATCGAGA CGGTGGCTGT GAGCATCCAC TTCCCAGACC AGAGCGATGT GGAGCTCAAG
    AGGCTGAACT CTCATCTGCA CTCCTGTGAC GAGGGGATGG CCATTCGCTG GCAGGTGGTG
    GATGAGCGCA TCCCCCTGTA TGACAACCAC AAGGTCATCC TCCAGAAAGT GGCCGCCTTG
    TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852512.1
    CDS1738..6135
    Translation

    Target ORF information:

    RefSeq Version XM_024996744.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996744.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGTGTA CAAAGGATAC ATGGATGACC CCAGGAACAC GGACAATGCC
    TGGATCGAGA CGGTGGCTGT GAGCATCCAC TTCCCAGACC AGAGCGATGT GGAGCTCAAG
    AGGCTGAACT CTCATCTGCA CTCCTGTGAC GAGGGGATGG CCATTCGCTG GCAGGTGGTG
    GATGAGCGCA TCCCCCTGTA TGACAACCAC AAGGTCATCC TCCAGAAAGT GGCCGCCTTG
    TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219298
    Clone ID Related Accession (Same CDS sequence) XM_024996748.1 , XM_024996756.1 , XM_024996764.1 , XM_024996758.1
    Accession Version XM_024996748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852516.1
    CDS657..4784
    Translation

    Target ORF information:

    RefSeq Version XM_024996748.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219298
    Clone ID Related Accession (Same CDS sequence) XM_024996748.1 , XM_024996756.1 , XM_024996764.1 , XM_024996758.1
    Accession Version XM_024996756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852524.1
    CDS478..4605
    Translation

    Target ORF information:

    RefSeq Version XM_024996756.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219298
    Clone ID Related Accession (Same CDS sequence) XM_024996748.1 , XM_024996756.1 , XM_024996764.1 , XM_024996758.1
    Accession Version XM_024996764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852532.1
    CDS389..4516
    Translation

    Target ORF information:

    RefSeq Version XM_024996764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa219298
    Clone ID Related Accession (Same CDS sequence) XM_024996748.1 , XM_024996756.1 , XM_024996764.1 , XM_024996758.1
    Accession Version XM_024996758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4128bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852526.1
    CDS218..4345
    Translation

    Target ORF information:

    RefSeq Version XM_024996758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGCCAACAG ACGCCTTCGG TGACATCGTC TTCACGGGCT TGGGCCAGAA AGTGGGAAAG 
    TATGTCCGCC TCTCCCAGGA CACTCCCTCC AGCCTGATTT ACCAGCTCAT GACCCAGCAG
    TGGGGCCTGG ATGCCCCCAA CCTGCTCATC TCCGTGACCG GCGGGGCCAA GGACTTCAAG
    ATGAAGCCCA GGCTGAAGAG CATTTTCCGA AGAGGGCTCC TCAAGGTGGC CCAGACCTCA
    GGGGCCTGGG TCATCACCGG GGGGTCCCAC ACTGGCGTAA TGAAGCAGGT GGGCGAGGCG
    GTGCGGAACT TCAGCCTGAG TGGCAGCTTC TGCGAAGGGG AAGTGGTCAC CATCGGGATC
    GCCACGTGGG GCGCCCTGCA CAACCGGGAG AGCCTGATCC ACCCCACGGG CGGCTTCCCT
    GCCGAGTACA TCATGGATGA GGGAGGCCAA GGGCACCTGA CCTGCCTGGA CAGCAACCAC
    TCCCATTTCA TTCTTGTGGA CGATGGGACC CACGGCCGCT ATGGGGTCGA GATTCCCCTG
    AGGACAAAGC TGGAGAAGTT CATATCGCAG CAGACCAAGG AGAGGGGAGG CGTGGCTATC
    AAGATCCCGA TTGTCTGCGT GGTGCTGGAA GGGGGCCCCG GCACACTGAA CACCATCTAC
    AATGCTATCA CCAACGGCAC CCCCTGCGTG GTTGTGGAGG GCTCGGGCCG CGTGGCTGAC
    GTCATCGCAC AGGTAGCCGG CCTGCCCATC TCTGAGATCA CGATCTCCCT GATTCAGCAG
    AAGCTGAGCA TGTTCTTCCA GGGGATGTTT GAGACCTTCA CTGAAAGTAA GATCGTGGAG
    TGGACCAAAA AGATTCAAGA CATCGTCCGG AGGCGGCAGC TGCTCACTGT GTTCCGGGAA
    GGGAAGGATG GCCAGCAGGA TGTGGACGTG GCCATCCTGC AGGCCTTGCT GAAAGCTTCT
    CGGAGCCTCG ACCACTTTGG CCATGAGAAC TGGGACCACC AGCTGAACCT GGCGGTGGCG
    TGGAACCGCG TGGACATTGC CCAGAGTGAG ATCTTCACCG ATGAGCGACA GTGGAAGCCC
    TCAGAGCTGT ACCCCATGAT GGTGGCCGCC CTCATCACCA ACAAGCCCGA GTTCGTGAAG
    CTCTTCCTGG AGAACGGGGT GCAGCTGAAG GAGTTCATCT CCCAGGCCAC CCTGTTCTAC
    CTGTACCAGA ACCTGGAGCC CACCTGCCTG TTCCACTGCA AGCTGGACAG GGTGCTTGGC
    GGGGAGCCTG AGCGCCTGGA CCCTGGTGTG CTCCTGCAGC GTGTGGCCCA GGTGCTCCGG
    GAGCTCCTCG GGGACTCCAC GCAGCTGCTA TACTCCCGGC CCCACTCCAG CGACCAGCAG
    CGCCTCTTCA CAGTGCCCAG CGTCAAAATC AGTGTGCAGG GAGTGAATCC CCAGTCCTCC
    TACAAGCATT CATCAAGCCA AGCTACCTTC ACCATAGATC CGGTCCGTGA TCTTCTCATT
    TGGGCCATCG TGCAGAACCG CCGGGAGCTG GCAGAAATCA TCTGGGCTCA GAGCCAGGAC
    TGCATTGCGG CAGCCTTGGC CTGCAGCAAG ATCCTAAAGG AGTTGTCCAA GGAGGAGGAG
    GACACGGACA GCTTGGAGGA GATGCTGGTG CTTGCGGACG AGTACGAACA GAGAGCCATT
    GGGGTCTTCA CTGAGTGCTA TCGGCAGAAT GAGGAGAGGG CTCAGAAGCT GCTGGTCCGC
    GTGTCGGAGG CCTGGGGCAG GACCACCTGC CTGCAGCTGG CCCTGGAGGC CAAGGACATG
    AAGTTCGTGT CTCACGGGGG CATCCAGGCC TACCTGACCA AGGTGTGGTG GGGCCAGCTC
    AGCGTGGACA ATGGGCTCTG GCGCCTGGTG CTGTGCATGT TGGTCTTCCC GCTGCTCTAC
    ACTGGCCTCG TCTCCTTCAG GAACCGGGAG CTGCAGGCAG ACAGGTGCCT GGCCCGCGTC
    CGCGCCTTCT TCAGCGCGCC CGTGGTCGTC TTCCACATGA ACATCCTGTC CTACTTCGCC
    TTCCTCTGCC TGTTTGCCTA CGTGCTCATG GTGGACTTCC AGCCCCAGCC CTCCAGCTGC
    GAGTGCCTCA TCTACGTCTG GCTCTTCTCC CTGGTGTGCG AGGAGCTGCG GCAGCTCTTC
    TATGACCCTG ACGGGAGCGG GCTGCGGAAG GCGGTGCTCG TGTACTTCAG TGACTACTGG
    AACAAGCTGG ACGTGTGCGC TATCCTGCTC TTCATTGCGG GGCTGACCTG CCGACTCATG
    CCCTCGCTGC TGTACACCGG GCGCATCATC CTCTCCCTGG ACTTCACCAT GTTCTGCCTG
    CGGCTCATGC ACATCTTCAC CGTCAGCAAG ACGCTGGGGC CCAAGATTAT CATCGTGAAG
    CGGATGATGA AGGACGTCTT CTTCTTCCTC TTCCTGCTGG CCGTGTGGGT TGTGTCCTTC
    GGGGTGGCCA AGCAGGCCAT CCTCATCCAC AACGAGAGGC GGGTGGAGTG GATCTTCCGG
    GGGGCCATCT ACCACTCGTA TCTCACCATC TTCGGGCAGC TGCCAACCTA CATTGACGGC
    GTGAACTTCA AACCAGACCA ATGTAGCCCC AATGGCACGG ATCCCTACAA GCCCAAGTGC
    CCCGAGATCG ACCAGGCGCG GCAGGAGCCC GTGTTCCCCG AGTGGCTGAC TGTCCTTCTG
    CTCTGCCTCT ACCTGCTCTT TGCCAACATC CTGCTGCTCA ACCTGCTCAT CGCCATGTTC
    AACTACACCT TCCAGCAGGT GCAGGAGCAC ACGGACCAGA TCTGGAAGTT CCAGCGCCAC
    GACCTGATCG AGGAGTACCA GGGCAGGCCC CCCGCGCCGC CCCCCTTCAT CCTCCTCAGC
    CACCTGCACC TCATCATCAA GAGGGTCGTG CTGAAGATCC CTGCCAAGCG GCATAAGCTG
    TTCAAGAACA AGCTAGAGAA GACAGAGGAG GCGGCCCTCC TGTCCTGGGA GATGTACCTG
    AAGGAGAACT ACCTGCAGGA ACAGCAGTTC CAGCAGAAGC AGAGGCCGGA GCAGAAGATC
    CAGGACGTCA GTGACAAGGT GGATTCCATG GTGCGCCTGC TGGAAATGGC ACATCTGAAG
    CAGTCGGGCG CCATGGAGCA GAGACTGGCC TCCCTGGAGG AGCAGGTGGC CCAGACAGCC
    ACAGCATTGC ACTGGATTGT GAAGGCCCTG AGGGACAGTG GCTTTGGCTC AGAAGAAGGT
    GTCCCCACTC TGGCACCCCA GAAGGCCTCA GTGGGGCGGG ATCCTGAGCT GGACAGCAGA
    TCGAAGGCAG AGGACCCAGA TGACGCCTAC CACGTGAATG CCCGGCATCT GCTCTACCCA
    AGTTGCTCTG TCCTGCGATT CCCGGTGCCC AACGAGAAGG TGCCCTGGGA GACGGAGTTC
    CTCATGTACA ACCCGCCCTT CTACACGGCC AACAGGAAGG ACAAGGACCT GGTGGACCCC
    GTGGGAGATG CCCTGGAACC TCTGTCCAGG ATCAGCTACA ATGCAGTGGA CGGACCACTG
    GACCGGCGCA GCTTCCATGG AGTCTACACA GTGCGGGACG GGCTCCCTCT AAACCCCATG
    GGTCGCACGG GACTGCGCGG CCGCGGGGAC CTCAGCCGCT TCGGCCCCAA TCACACACTG
    CAGCCTGTGA TCACCCGCTG GAGGCGGAGC CTGGATGGTG CCATCTGCAG GAAGAACGTC
    AAGAAGATGC TGGAGGTGCT GGTGGTGAAG CATGGCCCCT CCGAGCACTG GATGCTGCCT
    GGGGGCTCCC GGGAGCCAGG AGAGTTGCTG CCCCAGAATT TAAAACAGGT CCTGCAAAGA
    GAGTTCTGGT CTTCCTTCGA GCGCCTGCTG ACTCAGGGCA TGAAGGTGTA CAAAGGATAC
    ATGGATGACC CCAGGAACAC GGACAATGCC TGGATCGAGA CGGTGGCTGT GAGCATCCAC
    TTCCCAGACC AGAGCGATGT GGAGCTCAAG AGGCTGAACT CTCATCTGCA CTCCTGTGAC
    GAGGGGATGG CCATTCGCTG GCAGGTGGTG GATGAGCGCA TCCCCCTGTA TGACAACCAC
    AAGGTCATCC TCCAGAAAGT GGCCGCCTTG TTCATGGCCT ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87836
    Clone ID Related Accession (Same CDS sequence) XM_015462179.1 , XM_015473833.1
    Accession Version XM_015462179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGAGTTC TGCAGTCAGC
    TGTCCTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317665.1
    CDS1719..5690
    Translation

    Target ORF information:

    RefSeq Version XM_015462179.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGAGTTC TGCAGTCAGC
    TGTCCTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87836
    Clone ID Related Accession (Same CDS sequence) XM_015462179.1 , XM_015473833.1
    Accession Version XM_015473833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGAGTTC TGCAGTCAGC
    TGTCCTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329319.1
    CDS1719..5690
    Translation

    Target ORF information:

    RefSeq Version XM_015473833.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGAGTTC TGCAGTCAGC
    TGTCCTGGGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015473824.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329310.1
    CDS372..5033
    Translation

    Target ORF information:

    RefSeq Version XM_015473824.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473824.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015473825.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329311.1
    CDS196..4857
    Translation

    Target ORF information:

    RefSeq Version XM_015473825.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473825.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015473826.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329312.1
    CDS106..4767
    Translation

    Target ORF information:

    RefSeq Version XM_015473826.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473826.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015462174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317660.1
    CDS372..5033
    Translation

    Target ORF information:

    RefSeq Version XM_015462174.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015462175.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317661.1
    CDS196..4857
    Translation

    Target ORF information:

    RefSeq Version XM_015462175.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015462173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317659.1
    CDS1719..6380
    Translation

    Target ORF information:

    RefSeq Version XM_015462173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015462176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317662.1
    CDS106..4767
    Translation

    Target ORF information:

    RefSeq Version XM_015462176.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015473822.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329308.1
    CDS1719..6380
    Translation

    Target ORF information:

    RefSeq Version XM_015473822.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473822.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015462177.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317663.1
    CDS456..5117
    Translation

    Target ORF information:

    RefSeq Version XM_015462177.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87834
    Clone ID Related Accession (Same CDS sequence) XM_015473824.1 , XM_015473825.1 , XM_015473826.1 , XM_015462174.1 , XM_015462175.1 , XM_015462173.1 , XM_015462176.1 , XM_015473822.1 , XM_015462177.1 , XM_015473828.1
    Accession Version XM_015473828.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4662bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329314.1
    CDS456..5117
    Translation

    Target ORF information:

    RefSeq Version XM_015473828.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473828.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GGCTCCCGGC TGGGACCTCT GTTTCTGGGC ATGGTTCCGA TGGCCCTTCT GGCCAAGCCC
    CAGGTGTTGG GTGGCTGCCT GGGGAAGACG GGGGTGGGGA GCTGGGCATC CCGTGTGCGG
    GCACCTATTG ACCAATCTTG TTTTGAACGA GCATTCCAGG TGGCCCAGAC AGCCACAGCA
    TTGCACTGGA TTGTGAAGGC CCTGAGGGAC AGTGGCTTTG GCTCAGAAGA AGGTGTCCCC
    ACTCTGGCAC CCCAGAAGGC CTCAGTGGGG CGGGATCCTG AGCTGGACAG CAGATCGAAG
    GCAGAGGACC CAGATGACGC CTACCACGTG AATGCCCGGC ATCTGCTCTA CCCAAGTTGC
    TCTGTCCTGC GATTCCCGGT GCCCAACGAG AAGGTGCCCT GGGAGACGGA GTTCCTCATG
    TACAACCCGC CCTTCTACAC GGCCAACAGG AAGGACAAGG ACCTGGTGGA CCCCGTGGGA
    GATGCCCTGG AACCTCTGTC CAGGATCAGC TACAATGCAG TGGACGGACC ACTGGACCGG
    CGCAGCTTCC ATGGAGTCTA CACAGTGCGG GACGGGCTCC CTCTAAACCC CATGGGTCGC
    ACGGGACTGC GCGGCCGCGG GGACCTCAGC CGCTTCGGCC CCAATCACAC ACTGCAGCCT
    GTGATCACCC GCTGGAGGCG GAGCCTGGAT GGTGCCATCT GCAGGAAGAA CGTCAAGAAG
    ATGCTGGAGG TGCTGGTGGT GAAGCATGGC CCCTCCGAGC ACTGGATGCT GCCTGGGGGC
    TCCCGGGAGC CAGGAGAGTT GCTGCCCCAG AATTTAAAAC AGGTCCTGCA AAGAGAGTTC
    TGGTCTTCCT TCGAGCGCCT GCTGACTCAG GGCATGAAGG TGTACAAAGG ATACATGGAT
    GACCCCAGGA ACACGGACAA TGCCTGGATC GAGACGGTGG CTGTGAGCAT CCACTTCCCA
    GACCAGAGCG ATGTGGAGCT CAAGAGGCTG AACTCTCATC TGCACTCCTG TGACGAGGGG
    ATGGCCATTC GCTGGCAGGT GGTGGATGAG CGCATCCCCC TGTATGACAA CCACAAGGTC
    ATCCTCCAGA AAGTGGCCGC CTTGTTCATG GCCTACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87835
    Clone ID Related Accession (Same CDS sequence) XM_015473831.1 , XM_015462178.1
    Accession Version XM_015473831.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGGCTCCCG GCTGGGACCT
    CTGTTTCTGG GCATGGTTCC GATGGCCCTT CTGGCCAAGC CCCAGGTGTT GGGTGGCTGC
    CTGGGGAAGA CGGGGGTGGG GAGCTGGGCA TCCCGTGTGC GGGCACCTAT TGACCAATCT
    TGTTTTGAAC GAGCATTCCA GGTGGCCCAG ACAGCCACAG CATTGCACTG GATTGTGAAG
    GCCCTGAGGG ACAGTGGCTT TGGCTCAGAA GAAGGTGTCC CCACTCTGGC ACCCCAGAAG
    GCCTCAGTGG GGCGGGATCC TGAGCTGGAC AGCAGATCGA AGGCAGAGGA CCCAGATGAC
    GCCTACCACG TGAATGCCCG GCATCTGCTC TACCCAAGTT GCTCTGTCCT GCGATTCCCG
    GTGCCCAACG AGAAGGTGCC CTGGGAGACG GAGTTCCTCA TGTACAACCC GCCCTTCTAC
    ACGGCCAACA GGAAGGACAA GGACCTGGTG GACCCCGTGG GAGATGCCCT GGAACCTCTG
    TCCAGGATCA GCTACAATGC AGTGGACGGA CCACTGGACC GGCGCAGCTT CCATGGAGTC
    TACACAGTGC GGGACGGGCT CCCTCTAAAC CCCATGGGTC GCACGGGACT GCGCGGCCGC
    GGGGACCTCA GCCGCTTCGG CCCCAATCAC ACACTGCAGC CTGTGATCAC CCGCTGGAGG
    CGGAGCCTGG ATGGTGCCAT CTGCAGGAAG AACGTCAAGA AGATGCTGGA GGTGCTGGTG
    GTGAAGCATG GCCCCTCCGA GCACTGGATG CTGCCTGGGG GCTCCCGGGA GCCAGGAGAG
    TTGCTGCCCC AGAATTTAAA ACAGGTCCTG CAAAGAGAGT TCTGGTCTTC CTTCGAGCGC
    CTGCTGACTC AGGGCATGAA GGTGTACAAA GGATACATGG ATGACCCCAG GAACACGGAC
    AATGCCTGGA TCGAGACGGT GGCTGTGAGC ATCCACTTCC CAGACCAGAG CGATGTGGAG
    CTCAAGAGGC TGAACTCTCA TCTGCACTCC TGTGACGAGG GGATGGCCAT TCGCTGGCAG
    GTGGTGGATG AGCGCATCCC CCTGTATGAC AACCACAAGG TCATCCTCCA GAAAGTGGCC
    GCCTTGTTCA TGGCCTACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329317.1
    CDS1719..6362
    Translation

    Target ORF information:

    RefSeq Version XM_015473831.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473831.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGGCTCCCG GCTGGGACCT
    CTGTTTCTGG GCATGGTTCC GATGGCCCTT CTGGCCAAGC CCCAGGTGTT GGGTGGCTGC
    CTGGGGAAGA CGGGGGTGGG GAGCTGGGCA TCCCGTGTGC GGGCACCTAT TGACCAATCT
    TGTTTTGAAC GAGCATTCCA GGTGGCCCAG ACAGCCACAG CATTGCACTG GATTGTGAAG
    GCCCTGAGGG ACAGTGGCTT TGGCTCAGAA GAAGGTGTCC CCACTCTGGC ACCCCAGAAG
    GCCTCAGTGG GGCGGGATCC TGAGCTGGAC AGCAGATCGA AGGCAGAGGA CCCAGATGAC
    GCCTACCACG TGAATGCCCG GCATCTGCTC TACCCAAGTT GCTCTGTCCT GCGATTCCCG
    GTGCCCAACG AGAAGGTGCC CTGGGAGACG GAGTTCCTCA TGTACAACCC GCCCTTCTAC
    ACGGCCAACA GGAAGGACAA GGACCTGGTG GACCCCGTGG GAGATGCCCT GGAACCTCTG
    TCCAGGATCA GCTACAATGC AGTGGACGGA CCACTGGACC GGCGCAGCTT CCATGGAGTC
    TACACAGTGC GGGACGGGCT CCCTCTAAAC CCCATGGGTC GCACGGGACT GCGCGGCCGC
    GGGGACCTCA GCCGCTTCGG CCCCAATCAC ACACTGCAGC CTGTGATCAC CCGCTGGAGG
    CGGAGCCTGG ATGGTGCCAT CTGCAGGAAG AACGTCAAGA AGATGCTGGA GGTGCTGGTG
    GTGAAGCATG GCCCCTCCGA GCACTGGATG CTGCCTGGGG GCTCCCGGGA GCCAGGAGAG
    TTGCTGCCCC AGAATTTAAA ACAGGTCCTG CAAAGAGAGT TCTGGTCTTC CTTCGAGCGC
    CTGCTGACTC AGGGCATGAA GGTGTACAAA GGATACATGG ATGACCCCAG GAACACGGAC
    AATGCCTGGA TCGAGACGGT GGCTGTGAGC ATCCACTTCC CAGACCAGAG CGATGTGGAG
    CTCAAGAGGC TGAACTCTCA TCTGCACTCC TGTGACGAGG GGATGGCCAT TCGCTGGCAG
    GTGGTGGATG AGCGCATCCC CCTGTATGAC AACCACAAGG TCATCCTCCA GAAAGTGGCC
    GCCTTGTTCA TGGCCTACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa87835
    Clone ID Related Accession (Same CDS sequence) XM_015473831.1 , XM_015462178.1
    Accession Version XM_015462178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4644bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGGCTCCCG GCTGGGACCT
    CTGTTTCTGG GCATGGTTCC GATGGCCCTT CTGGCCAAGC CCCAGGTGTT GGGTGGCTGC
    CTGGGGAAGA CGGGGGTGGG GAGCTGGGCA TCCCGTGTGC GGGCACCTAT TGACCAATCT
    TGTTTTGAAC GAGCATTCCA GGTGGCCCAG ACAGCCACAG CATTGCACTG GATTGTGAAG
    GCCCTGAGGG ACAGTGGCTT TGGCTCAGAA GAAGGTGTCC CCACTCTGGC ACCCCAGAAG
    GCCTCAGTGG GGCGGGATCC TGAGCTGGAC AGCAGATCGA AGGCAGAGGA CCCAGATGAC
    GCCTACCACG TGAATGCCCG GCATCTGCTC TACCCAAGTT GCTCTGTCCT GCGATTCCCG
    GTGCCCAACG AGAAGGTGCC CTGGGAGACG GAGTTCCTCA TGTACAACCC GCCCTTCTAC
    ACGGCCAACA GGAAGGACAA GGACCTGGTG GACCCCGTGG GAGATGCCCT GGAACCTCTG
    TCCAGGATCA GCTACAATGC AGTGGACGGA CCACTGGACC GGCGCAGCTT CCATGGAGTC
    TACACAGTGC GGGACGGGCT CCCTCTAAAC CCCATGGGTC GCACGGGACT GCGCGGCCGC
    GGGGACCTCA GCCGCTTCGG CCCCAATCAC ACACTGCAGC CTGTGATCAC CCGCTGGAGG
    CGGAGCCTGG ATGGTGCCAT CTGCAGGAAG AACGTCAAGA AGATGCTGGA GGTGCTGGTG
    GTGAAGCATG GCCCCTCCGA GCACTGGATG CTGCCTGGGG GCTCCCGGGA GCCAGGAGAG
    TTGCTGCCCC AGAATTTAAA ACAGGTCCTG CAAAGAGAGT TCTGGTCTTC CTTCGAGCGC
    CTGCTGACTC AGGGCATGAA GGTGTACAAA GGATACATGG ATGACCCCAG GAACACGGAC
    AATGCCTGGA TCGAGACGGT GGCTGTGAGC ATCCACTTCC CAGACCAGAG CGATGTGGAG
    CTCAAGAGGC TGAACTCTCA TCTGCACTCC TGTGACGAGG GGATGGCCAT TCGCTGGCAG
    GTGGTGGATG AGCGCATCCC CCTGTATGAC AACCACAAGG TCATCCTCCA GAAAGTGGCC
    GCCTTGTTCA TGGCCTACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317664.1
    CDS1719..6362
    Translation

    Target ORF information:

    RefSeq Version XM_015462178.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGGACACTCC CTCCAGCCTG ATTTACCAGC TCATGACCCA GCAGTGGGGC
    CTGGATGCCC CCAACCTGCT CATCTCCGTG ACCGGCGGGG CCAAGGACTT CAAGATGAAG
    CCCAGGCTGA AGAGCATTTT CCGAAGAGGG CTCCTCAAGG TGGCCCAGAC CTCAGGGGCC
    TGGGTCATCA CCGGGGGGTC CCACACTGGC GTAATGAAGC AGGTGGGCGA GGCGGTGCGG
    AACTTCAGCC TGAGTGGCAG CTTCTGCGAA GGGGAAGTGG TCACCATCGG GATCGCCACG
    TGGGGCGCCC TGCACAACCG GGAGAGCCTG ATCCACCCCA CGGGCGGCTT CCCTGCCGAG
    TACATCATGG ATGAGGGAGG CCAAGGGCAC CTGACCTGCC TGGACAGCAA CCACTCCCAT
    TTCATTCTTG TGGACGATGG GACCCACGGC CGCTATGGGG TCGAGATTCC CCTGAGGACA
    AAGCTGGAGA AGTTCATATC GCAGCAGACC AAGGAGAGGG GAGGCGTGGC TATCAAGATC
    CCGATTGTCT GCGTGGTGCT GGAAGGGGGC CCCGGCACAC TGAACACCAT CTACAATGCT
    ATCACCAACG GCACCCCCTG CGTGGTTGTG GAGGGCTCGG GCCGCGTGGC TGACGTCATC
    GCACAGGTAG CCGGCCTGCC CATCTCTGAG ATCACGATCT CCCTGATTCA GCAGAAGCTG
    AGCATGTTCT TCCAGGGGAT GTTTGAGACC TTCACTGAAA GTAAGATCGT GGAGTGGACC
    AAAAAGATTC AAGACATCGT CCGGAGGCGG CAGCTGCTCA CTGTGTTCCG GGAAGGGAAG
    GATGGCCAGC AGGATGTGGA CGTGGCCATC CTGCAGGCCT TGCTGAAAGC TTCTCGGAGC
    CTCGACCACT TTGGCCATGA GAACTGGGAC CACCAGCTGA ACCTGGCGGT GGCGTGGAAC
    CGCGTGGACA TTGCCCAGAG TGAGATCTTC ACCGATGAGC GACAGTGGAA GCCCTCAGAG
    CTGTACCCCA TGATGGTGGC CGCCCTCATC ACCAACAAGC CCGAGTTCGT GAAGCTCTTC
    CTGGAGAACG GGGTGCAGCT GAAGGAGTTC ATCTCCCAGG CCACCCTGTT CTACCTGTAC
    CAGAACCTGG AGCCCACCTG CCTGTTCCAC TGCAAGCTGG ACAGGGTGCT TGGCGGGGAG
    CCTGAGCGCC TGGACCCTGG TGTGCTCCTG CAGCGTGTGG CCCAGGTGCT CCGGGAGCTC
    CTCGGGGACT CCACGCAGCT GCTATACTCC CGGCCCCACT CCAGCGACCA GCAGCGCCTC
    TTCACAGTGC CCAGCGTCAA AATCAGTGTG CAGGGAGTGA ATCCCCAGTC CTCCTACAAG
    CATTCATCAA GCCAAGCTAC CTTCACCATA GATCCGGTCC GTGATCTTCT CATTTGGGCC
    ATCGTGCAGA ACCGCCGGGA GCTGGCAGAA ATCATCTGGG CTCAGAGCCA GGACTGCATT
    GCGGCAGCCT TGGCCTGCAG CAAGATCCTA AAGGAGTTGT CCAAGGAGGA GGAGGACACG
    GACAGCTTGG AGGAGATGCT GGTGCTTGCG GACGAGTACG AACAGAGAGC CATTGGGGTC
    TTCACTGAGT GCTATCGGCA GAATGAGGAG AGGGCTCAGA AGCTGCTGGT CCGCGTGTCG
    GAGGCCTGGG GCAGGACCAC CTGCCTGCAG CTGGCCCTGG AGGCCAAGGA CATGAAGTTC
    GTGTCTCACG GGGGCATCCA GGCCTACCTG ACCAAGGTGT GGTGGGGCCA GCTCAGCGTG
    GACAATGGGC TCTGGCGCCT GGTGCTGTGC ATGTTGGTCT TCCCGCTGCT CTACACTGGC
    CTCGTCTCCT TCAGGAACCG GGAGCTGCAG GCAGACAGGT GCCTGGCCCG CGTCCGCGCC
    TTCTTCAGCG CGCCCGTGGT CGTCTTCCAC ATGAACATCC TGTCCTACTT CGCCTTCCTC
    TGCCTGTTTG CCTACGTGCT CATGGTGGAC TTCCAGCCCC AGCCCTCCAG CTGCGAGTGC
    CTCATCTACG TCTGGCTCTT CTCCCTGGTG TGCGAGGAGC TGCGGCAGCT CTTCTATGAC
    CCTGACGGGA GCGGGCTGCG GAAGGCGGTG CTCGTGTACT TCAGTGACTA CTGGAACAAG
    CTGGACGTGT GCGCTATCCT GCTCTTCATT GCGGGGCTGA CCTGCCGACT CATGCCCTCG
    CTGCTGTACA CCGGGCGCAT CATCCTCTCC CTGGACTTCA CCATGTTCTG CCTGCGGCTC
    ATGCACATCT TCACCGTCAG CAAGACGCTG GGGCCCAAGA TTATCATCGT GAAGCGGATG
    ATGAAGGACG TCTTCTTCTT CCTCTTCCTG CTGGCCGTGT GGGTTGTGTC CTTCGGGGTG
    GCCAAGCAGG CCATCCTCAT CCACAACGAG AGGCGGGTGG AGTGGATCTT CCGGGGGGCC
    ATCTACCACT CGTATCTCAC CATCTTCGGG CAGCTGCCAA CCTACATTGA CGGCGTGAAC
    TTCAAACCAG ACCAATGTAG CCCCAATGGC ACGGATCCCT ACAAGCCCAA GTGCCCCGAG
    ATCGACCAGG CGCGGCAGGA GCCCGTGTTC CCCGAGTGGC TGACTGTCCT TCTGCTCTGC
    CTCTACCTGC TCTTTGCCAA CATCCTGCTG CTCAACCTGC TCATCGCCAT GTTCAACTAC
    ACCTTCCAGC AGGTGCAGGA GCACACGGAC CAGATCTGGA AGTTCCAGCG CCACGACCTG
    ATCGAGGAGT ACCAGGGCAG GCCCCCCGCG CCGCCCCCCT TCATCCTCCT CAGCCACCTG
    CACCTCATCA TCAAGAGGGT CGTGCTGAAG ATCCCTGCCA AGCGGCATAA GCTGTTCAAG
    AACAAGCTAG AGAAGACAGA GGAGGCGGCC CTCCTGTCCT GGGAGATGTA CCTGAAGGAG
    AACTACCTGC AGGAACAGCA GTTCCAGCAG AAGCAGAGGC CGGAGCAGAA GATCCAGGAC
    GTCAGTGACA AGGTGGATTC CATGGTGCGC CTGCTGGAAA TGGCACATCT GAAGCAGTCG
    GGCGCCATGG AGCAGAGACT GGCCTCCCTG GAGGAGCAGG TGGGCTCCCG GCTGGGACCT
    CTGTTTCTGG GCATGGTTCC GATGGCCCTT CTGGCCAAGC CCCAGGTGTT GGGTGGCTGC
    CTGGGGAAGA CGGGGGTGGG GAGCTGGGCA TCCCGTGTGC GGGCACCTAT TGACCAATCT
    TGTTTTGAAC GAGCATTCCA GGTGGCCCAG ACAGCCACAG CATTGCACTG GATTGTGAAG
    GCCCTGAGGG ACAGTGGCTT TGGCTCAGAA GAAGGTGTCC CCACTCTGGC ACCCCAGAAG
    GCCTCAGTGG GGCGGGATCC TGAGCTGGAC AGCAGATCGA AGGCAGAGGA CCCAGATGAC
    GCCTACCACG TGAATGCCCG GCATCTGCTC TACCCAAGTT GCTCTGTCCT GCGATTCCCG
    GTGCCCAACG AGAAGGTGCC CTGGGAGACG GAGTTCCTCA TGTACAACCC GCCCTTCTAC
    ACGGCCAACA GGAAGGACAA GGACCTGGTG GACCCCGTGG GAGATGCCCT GGAACCTCTG
    TCCAGGATCA GCTACAATGC AGTGGACGGA CCACTGGACC GGCGCAGCTT CCATGGAGTC
    TACACAGTGC GGGACGGGCT CCCTCTAAAC CCCATGGGTC GCACGGGACT GCGCGGCCGC
    GGGGACCTCA GCCGCTTCGG CCCCAATCAC ACACTGCAGC CTGTGATCAC CCGCTGGAGG
    CGGAGCCTGG ATGGTGCCAT CTGCAGGAAG AACGTCAAGA AGATGCTGGA GGTGCTGGTG
    GTGAAGCATG GCCCCTCCGA GCACTGGATG CTGCCTGGGG GCTCCCGGGA GCCAGGAGAG
    TTGCTGCCCC AGAATTTAAA ACAGGTCCTG CAAAGAGAGT TCTGGTCTTC CTTCGAGCGC
    CTGCTGACTC AGGGCATGAA GGTGTACAAA GGATACATGG ATGACCCCAG GAACACGGAC
    AATGCCTGGA TCGAGACGGT GGCTGTGAGC ATCCACTTCC CAGACCAGAG CGATGTGGAG
    CTCAAGAGGC TGAACTCTCA TCTGCACTCC TGTGACGAGG GGATGGCCAT TCGCTGGCAG
    GTGGTGGATG AGCGCATCCC CCTGTATGAC AACCACAAGG TCATCCTCCA GAAAGTGGCC
    GCCTTGTTCA TGGCCTACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_024996733.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852501.1
    CDS179..4678
    Translation

    Target ORF information:

    RefSeq Version XM_024996733.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996733.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_024996726.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852494.1
    CDS367..4866
    Translation

    Target ORF information:

    RefSeq Version XM_024996726.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996726.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_010801661.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103825.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799963.1
    CDS1719..6218
    Translation

    Target ORF information:

    RefSeq Version XM_010801661.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801661.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_010801661.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799963.1
    CDS1737..6236
    Translation

    Target ORF information:

    RefSeq Version XM_010801661.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801661.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_024996739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852507.1
    CDS442..4941
    Translation

    Target ORF information:

    RefSeq Version XM_024996739.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_010824475.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644563.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010824475.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010822777.1
    CDS1719..6218
    Translation

    Target ORF information:

    RefSeq Version XM_010824475.2
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010824475.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16113
    Clone ID Related Accession (Same CDS sequence) XM_024996733.1 , XM_024996726.1 , XM_010801661.1 , XM_010801661.2 , XM_024996739.1 , XM_010824475.2 , XM_024996729.1
    Accession Version XM_024996729.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4500bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product transient receptor potential cation channel subfamily M member 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024852497.1
    CDS221..4720
    Translation

    Target ORF information:

    RefSeq Version XM_024996729.1
    Organism Bos taurus(cattle)
    Definition Bos taurus transient receptor potential cation channel subfamily M member 2 (TRPM2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024996729.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGTTAC TGGCGAAGAG GCATCGGGAT CAGGCCAGCT CCGGGGAGGA AGACAGCTTT 
    GGGGTGCAGC CCAGGAGGGT GGCCGACCTG GCCATGGTCC CCAACCTCCG GTGGAGCAGC
    ACCGGTCTCT GCAGGAGAAC GAGGTCGCAG TGCCTCTTCA GCAGCTGTGA AAAGCAAGGA
    GACCTGAGTT CATGGATTTC TGACAACATC AAGAAGAAAG AATGCATATA CTTTGTGGAA
    AGCTCCAAAC TGTCTGATGC TGGGAAGGTG GTGTGTGAGT GCGGCTACCC ACGTGAGCAG
    CACCTGGAGG AGGCCACGGG GTCCCACACC TTCCAGGGCC AGGAATGGGA CCCAAAAAAG
    CATGTCAGAG AGATGCCAAC AGACGCCTTC GGTGACATCG TCTTCACGGG CTTGGGCCAG
    AAAGTGGGAA AGTATGTCCG CCTCTCCCAG GACACTCCCT CCAGCCTGAT TTACCAGCTC
    ATGACCCAGC AGTGGGGCCT GGATGCCCCC AACCTGCTCA TCTCCGTGAC CGGCGGGGCC
    AAGGACTTCA AGATGAAGCC CAGGCTGAAG AGCATTTTCC GAAGAGGGCT CCTCAAGGTG
    GCCCAGACCT CAGGGGCCTG GGTCATCACC GGGGGGTCCC ACACTGGCGT AATGAAGCAG
    GTGGGCGAGG CGGTGCGGAA CTTCAGCCTG AGTGGCAGCT TCTGCGAAGG GGAAGTGGTC
    ACCATCGGGA TCGCCACGTG GGGCGCCCTG CACAACCGGG AGAGCCTGAT CCACCCCACG
    GGCGGCTTCC CTGCCGAGTA CATCATGGAT GAGGGAGGCC AAGGGCACCT GACCTGCCTG
    GACAGCAACC ACTCCCATTT CATTCTTGTG GACGATGGGA CCCACGGCCG CTATGGGGTC
    GAGATTCCCC TGAGGACAAA GCTGGAGAAG TTCATATCGC AGCAGACCAA GGAGAGGGGA
    GGCGTGGCTA TCAAGATCCC GATTGTCTGC GTGGTGCTGG AAGGGGGCCC CGGCACACTG
    AACACCATCT ACAATGCTAT CACCAACGGC ACCCCCTGCG TGGTTGTGGA GGGCTCGGGC
    CGCGTGGCTG ACGTCATCGC ACAGGTAGCC GGCCTGCCCA TCTCTGAGAT CACGATCTCC
    CTGATTCAGC AGAAGCTGAG CATGTTCTTC CAGGGGATGT TTGAGACCTT CACTGAAAGT
    AAGATCGTGG AGTGGACCAA AAAGATTCAA GACATCGTCC GGAGGCGGCA GCTGCTCACT
    GTGTTCCGGG AAGGGAAGGA TGGCCAGCAG GATGTGGACG TGGCCATCCT GCAGGCCTTG
    CTGAAAGCTT CTCGGAGCCT CGACCACTTT GGCCATGAGA ACTGGGACCA CCAGCTGAAC
    CTGGCGGTGG CGTGGAACCG CGTGGACATT GCCCAGAGTG AGATCTTCAC CGATGAGCGA
    CAGTGGAAGC CCTCAGAGCT GTACCCCATG ATGGTGGCCG CCCTCATCAC CAACAAGCCC
    GAGTTCGTGA AGCTCTTCCT GGAGAACGGG GTGCAGCTGA AGGAGTTCAT CTCCCAGGCC
    ACCCTGTTCT ACCTGTACCA GAACCTGGAG CCCACCTGCC TGTTCCACTG CAAGCTGGAC
    AGGGTGCTTG GCGGGGAGCC TGAGCGCCTG GACCCTGGTG TGCTCCTGCA GCGTGTGGCC
    CAGGTGCTCC GGGAGCTCCT CGGGGACTCC ACGCAGCTGC TATACTCCCG GCCCCACTCC
    AGCGACCAGC AGCGCCTCTT CACAGTGCCC AGCGTCAAAA TCAGTGTGCA GGGAGTGAAT
    CCCCAGTCCT CCTACAAGCA TTCATCAAGC CAAGCTACCT TCACCATAGA TCCGGTCCGT
    GATCTTCTCA TTTGGGCCAT CGTGCAGAAC CGCCGGGAGC TGGCAGAAAT CATCTGGGCT
    CAGAGCCAGG ACTGCATTGC GGCAGCCTTG GCCTGCAGCA AGATCCTAAA GGAGTTGTCC
    AAGGAGGAGG AGGACACGGA CAGCTTGGAG GAGATGCTGG TGCTTGCGGA CGAGTACGAA
    CAGAGAGCCA TTGGGGTCTT CACTGAGTGC TATCGGCAGA ATGAGGAGAG GGCTCAGAAG
    CTGCTGGTCC GCGTGTCGGA GGCCTGGGGC AGGACCACCT GCCTGCAGCT GGCCCTGGAG
    GCCAAGGACA TGAAGTTCGT GTCTCACGGG GGCATCCAGG CCTACCTGAC CAAGGTGTGG
    TGGGGCCAGC TCAGCGTGGA CAATGGGCTC TGGCGCCTGG TGCTGTGCAT GTTGGTCTTC
    CCGCTGCTCT ACACTGGCCT CGTCTCCTTC AGGAACCGGG AGCTGCAGGC AGACAGGTGC
    CTGGCCCGCG TCCGCGCCTT CTTCAGCGCG CCCGTGGTCG TCTTCCACAT GAACATCCTG
    TCCTACTTCG CCTTCCTCTG CCTGTTTGCC TACGTGCTCA TGGTGGACTT CCAGCCCCAG
    CCCTCCAGCT GCGAGTGCCT CATCTACGTC TGGCTCTTCT CCCTGGTGTG CGAGGAGCTG
    CGGCAGCTCT TCTATGACCC TGACGGGAGC GGGCTGCGGA AGGCGGTGCT CGTGTACTTC
    AGTGACTACT GGAACAAGCT GGACGTGTGC GCTATCCTGC TCTTCATTGC GGGGCTGACC
    TGCCGACTCA TGCCCTCGCT GCTGTACACC GGGCGCATCA TCCTCTCCCT GGACTTCACC
    ATGTTCTGCC TGCGGCTCAT GCACATCTTC ACCGTCAGCA AGACGCTGGG GCCCAAGATT
    ATCATCGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC TCTTCCTGCT GGCCGTGTGG
    GTTGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC ACAACGAGAG GCGGGTGGAG
    TGGATCTTCC GGGGGGCCAT CTACCACTCG TATCTCACCA TCTTCGGGCA GCTGCCAACC
    TACATTGACG GCGTGAACTT CAAACCAGAC CAATGTAGCC CCAATGGCAC GGATCCCTAC
    AAGCCCAAGT GCCCCGAGAT CGACCAGGCG CGGCAGGAGC CCGTGTTCCC CGAGTGGCTG
    ACTGTCCTTC TGCTCTGCCT CTACCTGCTC TTTGCCAACA TCCTGCTGCT CAACCTGCTC
    ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC ACACGGACCA GATCTGGAAG
    TTCCAGCGCC ACGACCTGAT CGAGGAGTAC CAGGGCAGGC CCCCCGCGCC GCCCCCCTTC
    ATCCTCCTCA GCCACCTGCA CCTCATCATC AAGAGGGTCG TGCTGAAGAT CCCTGCCAAG
    CGGCATAAGC TGTTCAAGAA CAAGCTAGAG AAGACAGAGG AGGCGGCCCT CCTGTCCTGG
    GAGATGTACC TGAAGGAGAA CTACCTGCAG GAACAGCAGT TCCAGCAGAA GCAGAGGCCG
    GAGCAGAAGA TCCAGGACGT CAGTGACAAG GTGGATTCCA TGGTGCGCCT GCTGGAAATG
    GCACATCTGA AGCAGTCGGG CGCCATGGAG CAGAGACTGG CCTCCCTGGA GGAGCAGGTG
    GCCCAGACAG CCACAGCATT GCACTGGATT GTGAAGGCCC TGAGGGACAG TGGCTTTGGC
    TCAGAAGAAG GTGTCCCCAC TCTGGCACCC CAGAAGGCCT CAGTGGGGCG GGATCCTGAG
    CTGGACAGCA GATCGAAGGC AGAGGACCCA GATGACGCCT ACCACGTGAA TGCCCGGCAT
    CTGCTCTACC CAAGTTGCTC TGTCCTGCGA TTCCCGGTGC CCAACGAGAA GGTGCCCTGG
    GAGACGGAGT TCCTCATGTA CAACCCGCCC TTCTACACGG CCAACAGGAA GGACAAGGAC
    CTGGTGGACC CCGTGGGAGA TGCCCTGGAA CCTCTGTCCA GGATCAGCTA CAATGCAGTG
    GACGGACCAC TGGACCGGCG CAGCTTCCAT GGAGTCTACA CAGTGCGGGA CGGGCTCCCT
    CTAAACCCCA TGGGTCGCAC GGGACTGCGC GGCCGCGGGG ACCTCAGCCG CTTCGGCCCC
    AATCACACAC TGCAGCCTGT GATCACCCGC TGGAGGCGGA GCCTGGATGG TGCCATCTGC
    AGGAAGAACG TCAAGAAGAT GCTGGAGGTG CTGGTGGTGA AGCATGGCCC CTCCGAGCAC
    TGGATGCTGC CTGGGGGCTC CCGGGAGCCA GGAGAGTTGC TGCCCCAGAA TTTAAAACAG
    GTCCTGCAAA GAGAGTTCTG GTCTTCCTTC GAGCGCCTGC TGACTCAGGG CATGAAGGTG
    TACAAAGGAT ACATGGATGA CCCCAGGAAC ACGGACAATG CCTGGATCGA GACGGTGGCT
    GTGAGCATCC ACTTCCCAGA CCAGAGCGAT GTGGAGCTCA AGAGGCTGAA CTCTCATCTG
    CACTCCTGTG ACGAGGGGAT GGCCATTCGC TGGCAGGTGG TGGATGAGCG CATCCCCCTG
    TATGACAACC ACAAGGTCAT CCTCCAGAAA GTGGCCGCCT TGTTCATGGC CTACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.