FREM1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM1
Entrez Gene ID	508018
Full Name	FRAS1 related extracellular matrix 1
General protein information	Preferred Names FRAS1 related extracellular matrix 1 Names FRAS1-related extracellular matrix protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005209921.4	XP_005209978.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_015472436.1	XP_015327922.1	FRAS1-related extracellular matrix protein 1 isoform X2
NM_001192995.1	NP_001179924.1	FRAS1-related extracellular matrix protein 1 precursor
XM_005209921.3	XP_005209978.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_010807797.2	XP_010806099.1	FRAS1-related extracellular matrix protein 1 isoform X1
XM_005209925.3	XP_005209982.1	FRAS1-related extracellular matrix protein 1 isoform X3
NM_001192995.2	NP_001179924.1	FRAS1-related extracellular matrix protein 1 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FREM1	XP_520492.3
Canis lupus familiaris (dog)	FREM1	XP_005626693.1
Homo sapiens (human)	FREM1	NP_659403.4
	FREM1	XP_001110764.1
Rattus norvegicus (Norway rat)	Frem1	NP_001178686.1
Bos taurus (cattle)	FREM1	NP_001179924.1
Danio rerio (zebrafish)	frem1a	NP_001177237.1
Mus musculus (house mouse)	Frem1	NP_001185740.1
Gallus gallus (chicken)	FREM1	XP_424932.2
Xenopus tropicalis (tropical clawed frog)	LOC100488523	XP_002939136.2

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FREM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa21928	XM_005209925.3 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa91204	XM_015472436.1 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
OBa01753	NM_001192995.1 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa21924	NM_001192995.2 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa21924	XM_005209921.4 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa21924	XM_010807797.2 Latest version!	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
View Old Accession Versions >>
OBa21924	XM_005209921.3	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM1 ( XM_005209925.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209982.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa21928
Clone ID Related Accession (Same CDS sequence)	XM_005209925.3
Accession Version	XM_005209925.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209925.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGACAC AAGAATCCAT GTTGAAGGCT GCCTTGCCTC TCTTTGCCAG ATTCATCGTC 
AGCAATGGAC TTCAGACCAA GCCTGGGGTT TTTGAGATCA CACTGGAGAC TGTGGACACA 
GCCTTACCCG TGGTGACCAG GAACAAGGGG TTGAGACTGG TTGAAGGGGC CACGGGCCTG 
CTCTCCCCTG ACCTCCTGCA GTTGACTGAC CCTGACACTC CAGCCGAGAA GCTCACCTTT 
CTTCTGGCCC AGCTCCCACG GCATGGCCAG CTCTACCTGC GGGGGATGGC GCTGATCCCA 
CACAATTTCA CTCAGCTGGA CGTGGACAGT GGGCATGTGG CCTATCGGCA CTTGGGAGGG 
GATTCTTGGA CTGACCACTT CACTTTTGTG GCCACGGATG GGACGAACCA TGGCTTTCTT 
GTGGATGGGA GAGTGTGGCA AGAACCTGTA TCATTCACCA TCCAGGTGGA CCAGTTGGAC 
AAAGCGGCCC CCCGTGTCAC ACACTTGCAT TCCCCTTCTC AAGTAGGGCT CTTGAAAAAT 
GGTTGTTATG GGATTTACAT CACCTCCCGT GTGTTGAAGG CATCAGACCC CGACACTGAT 
GATAATCAGA TCATCTTTAA GATTTTACGA GGCCCCCAAC ATGGGCATCT GGAGAACTCA 
ACGACAGGTG AATTTATCCA TGAGAAATTT AGCCAGAAGG ATTTAAACAG TAAGACCGTT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCATGGAATT TCAAGTCATG 
GATCCCACAG GGAACTCTGC CACTCCTCAA AGTTTGGAAC TGAATTGGTC TTATATTGAG 
TGGTCGCGGA CTGAATATGA GGTCTGTGAG AATATGGGCA TGCTACCCTT GGAGATTACC 
AGAAGGGGAT ATTCCATGGA TTCAGCCTTT GTGACTGTAC AGGTCAACCA AGTGTCAGCT 
ACAGTTGGAA AGGATTTCAC CATGACTCCA TCTAAGCTGG TGCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCCGTGAAT GCGGTTCTTG GCACAAAGAC AAAAACTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGGCAG TGCCATCCTT CAAATGCCTT CAATTCAAAC 
ATGCCCAGTG CATGGGAGAA GGGCATTTGG CACCTGCTCT CCCCAGGGGC TTCCTCACCC 
ACCACTTCTG ATTCCTTTCA TCTGGAAAGA AGACCCCTTC CAACTTCCAA GCAGCTAGCG 
GTCACCAGGG GTGACGCCCT GCGGGACTTT GGTTCTGAAG ATCTTTCTCC AGTGAAGCTT 
AGGACCCGAG GGGATGGCAA AACAGTTCCT CCATCCTCTG TTTATAAAAA TAGAACAGAC 
ATCATCTATA ACTATCATGG GGTGATTTCC CTGAAACTGG AAGATGACGG CTCCCCAGCT 
CGCAGAAGGA AGGCCAATGT ATCCTTTATT AGTGAGCCCC AAAAGACAAC CAAAGTGGCA 
GAACCGCCTC AAGCCGATAA GGAGGAATCC ACAACCGGCT CACACTTCCC CAGACAGGAC 
CCGTTGCCCT CATTCCCAAA GAACTGCACA CCGGAATTAA AGGGACTCTT CCACTTTGAA 
GAAAGCCTCC AAAAACTGTA CAAGTGCGAT GGAATTGCCT GGAGATCCTG GAGCCCCCAC 
ACCAAGGAGG TGGAAGATAA ATCCTGCCCA GCTGGGTGGC ACCATCATTC AGGCTACTGC 
CACTCCTTGA TCACAGAGCA GAAAGGCACG TGGACTACAG CTGCCCAAGC TTGCAGGGAA 
CATTACCAAG GCAGCCTTGT AACTGTCATC TCCAGGCAGC ACATGAGATG GCTTTGGGAC 
ATCAGTGGAA GAAAGCCGTT TTGGATAGGT TTGAATGACC AAGTGCGTGC TGGCCACTGG 
GAGTGGATTG GCGGTGAACC TGTGACCTTC ACCAACTGGA GGAGAGGGCC CCCGCAACGT 
TCAAGGCATG GCAAGGACTG TGTTTTGGTT CAAAGGCCAG GAAAATGGCA CACAAAGGAC 
TGTAGGAAGG GAAAATCTCA CAGTTATGTG TGCTCCAGGA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209982.1
CDS	101..2248
Translation	MVTQESMLKAALPLFARFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	XM_005209925.3
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209925.3

ORF Insert Sequence:

ATGGTGACAC AAGAATCCAT GTTGAAGGCT GCCTTGCCTC TCTTTGCCAG ATTCATCGTC 
AGCAATGGAC TTCAGACCAA GCCTGGGGTT TTTGAGATCA CACTGGAGAC TGTGGACACA 
GCCTTACCCG TGGTGACCAG GAACAAGGGG TTGAGACTGG TTGAAGGGGC CACGGGCCTG 
CTCTCCCCTG ACCTCCTGCA GTTGACTGAC CCTGACACTC CAGCCGAGAA GCTCACCTTT 
CTTCTGGCCC AGCTCCCACG GCATGGCCAG CTCTACCTGC GGGGGATGGC GCTGATCCCA 
CACAATTTCA CTCAGCTGGA CGTGGACAGT GGGCATGTGG CCTATCGGCA CTTGGGAGGG 
GATTCTTGGA CTGACCACTT CACTTTTGTG GCCACGGATG GGACGAACCA TGGCTTTCTT 
GTGGATGGGA GAGTGTGGCA AGAACCTGTA TCATTCACCA TCCAGGTGGA CCAGTTGGAC 
AAAGCGGCCC CCCGTGTCAC ACACTTGCAT TCCCCTTCTC AAGTAGGGCT CTTGAAAAAT 
GGTTGTTATG GGATTTACAT CACCTCCCGT GTGTTGAAGG CATCAGACCC CGACACTGAT 
GATAATCAGA TCATCTTTAA GATTTTACGA GGCCCCCAAC ATGGGCATCT GGAGAACTCA 
ACGACAGGTG AATTTATCCA TGAGAAATTT AGCCAGAAGG ATTTAAACAG TAAGACCGTT 
CTTTACATCA TAAACCCATC TTTGGAAGTA AATTCAGATA CCATGGAATT TCAAGTCATG 
GATCCCACAG GGAACTCTGC CACTCCTCAA AGTTTGGAAC TGAATTGGTC TTATATTGAG 
TGGTCGCGGA CTGAATATGA GGTCTGTGAG AATATGGGCA TGCTACCCTT GGAGATTACC 
AGAAGGGGAT ATTCCATGGA TTCAGCCTTT GTGACTGTAC AGGTCAACCA AGTGTCAGCT 
ACAGTTGGAA AGGATTTCAC CATGACTCCA TCTAAGCTGG TGCAGTTTGA CCCAGGAATG 
TCAACTAAGA TGTGGAATAT AGCAATTACC TATGACGGAT TAGAGGAAGA TGATGAGGTC 
TTTGAAGTAA TTCTGAACTC CCCCGTGAAT GCGGTTCTTG GCACAAAGAC AAAAACTGCA 
GTGAAAATTT TGGACTCAAA AGGAGGGCAG TGCCATCCTT CAAATGCCTT CAATTCAAAC 
ATGCCCAGTG CATGGGAGAA GGGCATTTGG CACCTGCTCT CCCCAGGGGC TTCCTCACCC 
ACCACTTCTG ATTCCTTTCA TCTGGAAAGA AGACCCCTTC CAACTTCCAA GCAGCTAGCG 
GTCACCAGGG GTGACGCCCT GCGGGACTTT GGTTCTGAAG ATCTTTCTCC AGTGAAGCTT 
AGGACCCGAG GGGATGGCAA AACAGTTCCT CCATCCTCTG TTTATAAAAA TAGAACAGAC 
ATCATCTATA ACTATCATGG GGTGATTTCC CTGAAACTGG AAGATGACGG CTCCCCAGCT 
CGCAGAAGGA AGGCCAATGT ATCCTTTATT AGTGAGCCCC AAAAGACAAC CAAAGTGGCA 
GAACCGCCTC AAGCCGATAA GGAGGAATCC ACAACCGGCT CACACTTCCC CAGACAGGAC 
CCGTTGCCCT CATTCCCAAA GAACTGCACA CCGGAATTAA AGGGACTCTT CCACTTTGAA 
GAAAGCCTCC AAAAACTGTA CAAGTGCGAT GGAATTGCCT GGAGATCCTG GAGCCCCCAC 
ACCAAGGAGG TGGAAGATAA ATCCTGCCCA GCTGGGTGGC ACCATCATTC AGGCTACTGC 
CACTCCTTGA TCACAGAGCA GAAAGGCACG TGGACTACAG CTGCCCAAGC TTGCAGGGAA 
CATTACCAAG GCAGCCTTGT AACTGTCATC TCCAGGCAGC ACATGAGATG GCTTTGGGAC 
ATCAGTGGAA GAAAGCCGTT TTGGATAGGT TTGAATGACC AAGTGCGTGC TGGCCACTGG 
GAGTGGATTG GCGGTGAACC TGTGACCTTC ACCAACTGGA GGAGAGGGCC CCCGCAACGT 
TCAAGGCATG GCAAGGACTG TGTTTTGGTT CAAAGGCCAG GAAAATGGCA CACAAAGGAC 
TGTAGGAAGG GAAAATCTCA CAGTTATGTG TGCTCCAGGA AACTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_015472436.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015327922.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa91204
Clone ID Related Accession (Same CDS sequence)	XM_015472436.1
Accession Version	XM_015472436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104087.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAT ATCATGGGGT GATTTCCCTG 
AAACTGGAAG ATGACGGCTC CCCAGCTCGC AGAAGGAAGG CCAATGTATC CTTTATTAGT 
GAGCCCCAAA AGACAACCAA AGTGGCAGAA CCGCCTCAAG CCGATAAGGA GGAATCCACA 
ACCGGCTCAC ACTTCCCCAG ACAGGACCCG TTGCCCTCAT TCCCAAAGAA CTGCACACCG 
GAATTAAAGG GACTCTTCCA CTTTGAAGAA AGCCTCCAAA AACTGTACAA GTGCGATGGA 
ATTGCCTGGA GATCCTGGAG CCCCCACACC AAGGAGGTGG AAGATAAATC CTGCCCAGCT 
GGGTGGCACC ATCATTCAGG CTACTGCCAC TCCTTGATCA CAGAGCAGAA AGGCACGTGG 
ACTACAGCTG CCCAAGCTTG CAGGGAACAT TACCAAGGCA GCCTTGTAAC TGTCATCTCC 
AGGCAGCACA TGAGATGGCT TTGGGACATC AGTGGAAGAA AGCCGTTTTG GATAGGTTTG 
AATGACCAAG TGCGTGCTGG CCACTGGGAG TGGATTGGCG GTGAACCTGT GACCTTCACC 
AACTGGAGGA GAGGGCCCCC GCAACGTTCA AGGCATGGCA AGGACTGTGT TTTGGTTCAA 
AGGCCAGGAA AATGGCACAC AAAGGACTGT AGGAAGGGAA AATCTCACAG TTATGTGTGC 
TCCAGGAAAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015327922.1
CDS	789..7283
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	XM_015472436.1
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015472436.1

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAT ATCATGGGGT GATTTCCCTG 
AAACTGGAAG ATGACGGCTC CCCAGCTCGC AGAAGGAAGG CCAATGTATC CTTTATTAGT 
GAGCCCCAAA AGACAACCAA AGTGGCAGAA CCGCCTCAAG CCGATAAGGA GGAATCCACA 
ACCGGCTCAC ACTTCCCCAG ACAGGACCCG TTGCCCTCAT TCCCAAAGAA CTGCACACCG 
GAATTAAAGG GACTCTTCCA CTTTGAAGAA AGCCTCCAAA AACTGTACAA GTGCGATGGA 
ATTGCCTGGA GATCCTGGAG CCCCCACACC AAGGAGGTGG AAGATAAATC CTGCCCAGCT 
GGGTGGCACC ATCATTCAGG CTACTGCCAC TCCTTGATCA CAGAGCAGAA AGGCACGTGG 
ACTACAGCTG CCCAAGCTTG CAGGGAACAT TACCAAGGCA GCCTTGTAAC TGTCATCTCC 
AGGCAGCACA TGAGATGGCT TTGGGACATC AGTGGAAGAA AGCCGTTTTG GATAGGTTTG 
AATGACCAAG TGCGTGCTGG CCACTGGGAG TGGATTGGCG GTGAACCTGT GACCTTCACC 
AACTGGAGGA GAGGGCCCCC GCAACGTTCA AGGCATGGCA AGGACTGTGT TTTGGTTCAA 
AGGCCAGGAA AATGGCACAC AAAGGACTGT AGGAAGGGAA AATCTCACAG TTATGTGTGC 
TCCAGGAAAC TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001192995.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179924.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.

Price & Availability↑

CloneID	OBa01753
Clone ID Related Accession (Same CDS sequence)	NM_001192995.1
Accession Version	NM_001192995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-13
Organism	Bos taurus(Cattle)
Product	FRAS1-related extracellular matrix protein 1 precursor
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03090351.1, AAFC03090350.1 and AAFC03017627.1. On Jul 17, 2010 this sequence version replaced XM_865053.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACTC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCCTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179924.1
CDS	1..6543
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	NM_001192995.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192995.1

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACTC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCCTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( NM_001192995.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001179924.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.

Price & Availability↑

CloneID	OBa21924
Clone ID Related Accession (Same CDS sequence)	XM_005209921.4 , XM_005209921.3 , XM_010807797.2 , NM_001192995.2
Accession Version	NM_001192995.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000008.1. On Jun 30, 2017 this sequence version replaced NM_001192995.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145425, SAMN03145448 [ECO:0000348] ##Evidence-Data-END##

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001179924.1
CDS	1..6543
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	NM_001192995.2
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001192995.2

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_005209921.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209978.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21924
Clone ID Related Accession (Same CDS sequence)	XM_005209921.4 , XM_005209921.3 , XM_010807797.2 , NM_001192995.2
Accession Version	XM_005209921.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037335.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209921.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209978.1
CDS	841..7383
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	XM_005209921.4
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209921.4

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_005209921.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209978.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa21924
Clone ID Related Accession (Same CDS sequence)	XM_005209921.4 , XM_005209921.3 , XM_010807797.2 , NM_001192995.2
Accession Version	XM_005209921.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209921.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209978.1
CDS	789..7331
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	XM_005209921.3
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209921.3

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM1 ( XM_010807797.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010806099.1 Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa21924
Clone ID Related Accession (Same CDS sequence)	XM_005209921.4 , XM_005209921.3 , XM_010807797.2 , NM_001192995.2
Accession Version	XM_010807797.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	FRAS1-related extracellular matrix protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104087.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807797.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010806099.1
CDS	547..7089
Translation	MSSPSWGGLGALLLLLLLPGWASPTFVRVNQGVRVMKGSSAFLSQDDLKFTIPKEKDACKVEVVMNEPITQRVGKLTPQVFDCHFLPNEVKYTHNGCPILDEDTVKLRLYRFTETDTFTETFILRVYLLEPDCNIIRMSDNALEVSEFYGLSRVIDKNLLRFDYERMASLECTVRLDPVGTHLPAHGQLVLVEPQLEEPRGDQPHSFFPESQLGTELKCPGGRCTLGLKKVGSLKVSCEEFLLMGLRYQHLGSPSPNIDYFSIQLDLTDSRSKISYKSESAWLPVYIRPGIPNQIPRAAFMAMFILEVDQFILTSLTTSVLDCEEDETPKPLLVFNITKAPLQGYVTHLWDHTRPVSSFTWKDLRDMQIAYQPPNSSHSERRLYEVELEVYDFFFEKSAPITVHISIRTTDTNAPRVSWNTGLNLLEGQSRAITWEQFQIVDNDDIHAVQVVTVDGLQHGRLTVRGGKGFLFTVADLQAGVVRYHHDDSDSTKDFVVFRIFDSQHSIQHKFPINILPKDDSPPFLITNVVIELEEGQTILIQGSMLRASDMDSSDDYIFFNITKPPQAGEIMKKPAPGLIGYPVPGFIQRDLFNGIIYYRHFGGEIFEDSFEFVLWDSHEPPNLSVPQVVTIHVTPVNDQLPKEAPGVSRHLVVKETEVAYITKKHLHFIDTESQDRELLYTITTPPFFSSSRRHLDAGKLFMVDSIPRLTKTPAAPGLKYFTQHAVNHMKVAYMPPMQDVGPHPRHVQFAFSVSNQHGGTLYGICFNITILPVDNQAPKVFTKPLRVAEGGQCVISTQHVLVSDEDTKLDNIYLSLQSPPQHGMVELNGFPLNKGTTFPWGVLQALKLRYQHDGSEELQDDIVLQVTDGTNTAEYVLHVEVFPVNDEPPVLKADLIPMMHCSEGEEVVITSEYIFATDVDSDDLKLIFMIVGEPQHGTVRRAGVTVHQFSQEDVVAGAVTYKHTGGEIGLMPCFDTITLVVSDREAGPFVNGCCYNGPHPSVPLHESFPMYDLNITVYPVDNQPPSVAIGTTFVVDEGFSAALTVNHLSATDPDTAADDLEFVLVSPPQFGYLENTLPSAGFEKSNMGISIASFQWKDMKALHINYVQSRHLGMEPTADKFVLYVTDGKRRSMEIPFYIIINATNDEAPDFIVQNITVCEGQMRELDSSIISATDRDIPKNPLLFSITHKPRHGLLINRAFSRNFFQYKQLANPGQKHELVYNFSMELLKNGMRLVYVHDDSESLADDFTIQVSDGKHKILKTIFVEVTPVNDEKPMLSKKAEITINMGETRIISSAVLSAIDEDSPREKIYYIFERIPQNGQLQLKIGRDWVPLYPGMKCTQEEVDLNLLRYTHTGAMDSKNQDSFTFRLWDGDNRSPAFDCHITIKNMGKGDIVILAKPLVVSKGDRGFLTTTTLLAVDGTDKPEELLYVITSPPQFGQVEYVRYPGVPITSFSQMDIAGQTVCYVDKSTTAVHRDTFRFIVSNGLQTKPGVFEITLETVDTALPVVTRNKGLRLVEGATGLLSPDLLQLTDPDTPAEKLTFLLAQLPRHGQLYLRGMALIPHNFTQLDVDSGHVAYRHLGGDSWTDHFTFVATDGTNHGFLVDGRVWQEPVSFTIQVDQLDKAAPRVTHLHSPSQVGLLKNGCYGIYITSRVLKASDPDTDDNQIIFKILRGPQHGHLENSTTGEFIHEKFSQKDLNSKTVLYIINPSLEVNSDTMEFQVMDPTGNSATPQSLELNWSYIEWSRTEYEVCENMGMLPLEITRRGYSMDSAFVTVQVNQVSATVGKDFTMTPSKLVQFDPGMSTKMWNIAITYDGLEEDDEVFEVILNSPVNAVLGTKTKTAVKILDSKGGQCHPSNAFNSNMPSAWEKGIWHLLSPGASSPTTSDSFHLERRPLPTSKQLAVTRGDALRDFGSEDLSPVKLRTRGDGKTVPPSSVYKNRTDIIYNYHGVISLKLEDDGSPARRRKANVSFISEPQKTTKVAEPPQADKEESTTGSHFPRQDPLPSFPKNCTPELKGLFHFEESLQKLYKCDGIAWRSWSPHTKEVEDKSCPAGWHHHSGYCHSLITEQKGTWTTAAQACREHYQGSLVTVISRQHMRWLWDISGRKPFWIGLNDQVRAGHWEWIGGEPVTFTNWRRGPPQRSRHGKDCVLVQRPGKWHTKDCRKGKSHSYVCSRKL

Target ORF information:

RefSeq Version	XM_010807797.2
Organism	Bos taurus(cattle)
Definition	Bos taurus FRAS1 related extracellular matrix 1 (FREM1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807797.2

ORF Insert Sequence:

ATGAGCTCTC CAAGCTGGGG TGGGCTTGGT GCCCTGCTCC TGCTGCTGCT GCTCCCAGGC 
TGGGCCAGCC CCACCTTTGT CCGTGTTAAC CAAGGGGTCA GGGTGATGAA GGGCAGTTCC 
GCCTTCCTGT CTCAAGATGA CCTGAAGTTC ACCATCCCCA AAGAGAAAGA TGCCTGCAAA 
GTGGAAGTCG TGATGAATGA GCCAATAACC CAGAGGGTTG GGAAACTCAC TCCACAGGTC 
TTTGACTGCC ATTTCCTTCC CAATGAAGTA AAATATACTC ATAACGGTTG CCCAATTCTT 
GATGAAGACA CAGTGAAACT TAGACTTTAC AGGTTTACTG AGACAGACAC CTTCACGGAA 
ACCTTTATCC TGCGGGTCTA CCTCCTGGAA CCAGACTGTA ACATCATCCG CATGAGTGAT 
AATGCACTGG AGGTGTCTGA GTTCTATGGC CTGTCCCGAG TCATTGATAA GAACCTGCTT 
AGATTTGATT ATGAGAGGAT GGCCAGCCTG GAATGCACAG TCAGACTGGA CCCTGTGGGA 
ACTCACCTGC CAGCCCACGG CCAGTTGGTT CTGGTGGAGC CCCAGCTGGA AGAACCTCGC 
GGAGATCAGC CACATAGTTT CTTCCCTGAG TCTCAATTGG GAACAGAGCT GAAGTGTCCA 
GGCGGCAGGT GTACCCTGGG ACTGAAGAAA GTCGGAAGTC TCAAAGTGAG CTGTGAGGAG 
TTCCTGCTGA TGGGGCTTCG ATACCAGCAC CTGGGTTCCC CTTCTCCCAA CATTGATTAC 
TTCTCCATCC AACTGGACCT CACAGATAGC AGGAGTAAAA TCTCATACAA GTCAGAGAGT 
GCGTGGTTGC CTGTCTATAT CAGACCTGGA ATTCCCAATC AGATTCCAAG AGCTGCATTT 
ATGGCCATGT TTATTCTGGA AGTGGATCAG TTCATCCTGA CCTCATTGAC CACATCGGTT 
CTGGACTGTG AAGAGGATGA GACCCCCAAA CCCTTGCTGG TGTTCAACAT TACTAAAGCC 
CCACTCCAGG GCTATGTGAC TCACCTGTGG GATCACACCA GACCAGTGTC CTCCTTCACC 
TGGAAGGATC TAAGGGATAT GCAGATTGCC TATCAGCCAC CAAACAGCAG CCATTCTGAG 
AGGAGACTTT ATGAGGTGGA GTTGGAGGTC TATGACTTCT TCTTTGAGAA GAGTGCACCT 
ATTACAGTCC ACATTTCCAT CAGAACAACA GATACAAATG CCCCGCGAGT GTCCTGGAAC 
ACAGGTCTGA ATCTCCTGGA GGGACAGTCT AGGGCCATCA CATGGGAACA GTTTCAGATT 
GTGGACAATG ATGACATTCA TGCTGTCCAG GTAGTCACTG TGGATGGCCT GCAGCATGGA 
CGGCTTACTG TGAGAGGGGG GAAGGGGTTT CTCTTCACCG TGGCTGACCT TCAGGCTGGA 
GTCGTTCGAT ATCATCATGA TGACAGTGAC TCCACCAAAG ACTTTGTGGT CTTCAGGATA 
TTTGACAGCC AGCACAGCAT CCAACATAAG TTTCCCATCA ACATCTTGCC CAAAGATGAC 
AGTCCGCCAT TTCTCATAAC AAATGTTGTG ATTGAACTGG AGGAGGGGCA GACCATACTA 
ATCCAGGGTT CCATGCTGAG AGCTTCAGAC ATGGACTCCA GTGATGACTA CATCTTCTTT 
AATATCACGA AACCCCCACA AGCTGGAGAG ATCATGAAGA AACCAGCACC AGGACTGATA 
GGCTATCCAG TCCCTGGCTT CATTCAGAGG GATCTATTCA ATGGTATTAT TTACTATCGT 
CACTTTGGTG GAGAAATCTT TGAAGATTCA TTTGAATTTG TCCTGTGGGA CAGCCACGAA 
CCTCCAAATC TCTCAGTGCC ACAGGTGGTG ACAATCCATG TCACTCCAGT AAATGACCAG 
CTTCCAAAAG AGGCTCCTGG AGTTTCTCGG CATTTAGTTG TCAAGGAAAC GGAGGTGGCC 
TACATCACTA AGAAACATCT ACATTTTATA GATACAGAAT CACAAGACAG GGAGCTGCTC 
TATACAATAA CCACTCCTCC ATTTTTCTCC TCCAGCCGCA GACACTTAGA TGCTGGGAAA 
TTGTTCATGG TGGACAGCAT ACCAAGGCTA ACCAAGACTC CTGCAGCTCC AGGGTTAAAG 
TATTTCACGC AGCACGCTGT GAACCATATG AAAGTGGCCT ACATGCCACC CATGCAAGAT 
GTAGGTCCGC ATCCAAGACA CGTCCAGTTT GCCTTTTCCG TCAGTAACCA GCACGGTGGG 
ACGCTGTATG GGATCTGCTT TAACATCACG ATTCTTCCGG TGGACAATCA GGCCCCAAAG 
GTGTTTACCA AACCTCTGAG AGTGGCTGAG GGAGGTCAAT GTGTCATCAG CACACAGCAT 
GTCCTGGTTT CTGATGAGGA CACCAAACTG GACAACATCT ACCTCTCCTT GCAGAGTCCA 
CCTCAGCATG GAATGGTGGA GCTAAATGGA TTCCCTCTAA ACAAAGGGAC CACGTTTCCT 
TGGGGAGTCC TCCAGGCCTT AAAACTTAGA TATCAGCATG ATGGATCCGA GGAACTTCAG 
GATGACATAG TACTGCAGGT CACAGATGGC ACAAACACAG CTGAATATGT CCTACACGTT 
GAGGTGTTCC CTGTAAATGA CGAGCCACCA GTGCTAAAGG CGGACCTCAT TCCCATGATG 
CATTGCTCCG AGGGGGAAGA GGTTGTCATC ACTTCTGAAT ACATTTTTGC TACTGATGTG 
GACAGTGATG ATTTAAAGCT GATATTCATG ATTGTTGGAG AACCTCAGCA TGGGACCGTA 
AGGAGAGCTG GGGTCACAGT ACATCAGTTC TCTCAGGAAG ATGTTGTCGC AGGGGCTGTG 
ACGTACAAGC ACACAGGTGG TGAGATTGGC CTGATGCCTT GTTTCGACAC CATTACATTG 
GTGGTTTCGG ACAGAGAAGC TGGTCCTTTT GTGAATGGCT GCTGTTACAA TGGACCTCAT 
CCATCTGTTC CTCTTCATGA GTCTTTTCCA ATGTATGATC TCAACATCAC AGTGTATCCA 
GTGGATAACC AACCACCTTC AGTTGCCATA GGTACCACAT TCGTTGTAGA TGAGGGCTTC 
TCAGCAGCCC TTACTGTTAA CCATCTGTCT GCCACTGATC CTGACACTGC AGCAGATGAC 
TTGGAATTTG TTTTGGTTTC TCCTCCCCAG TTTGGCTACC TTGAAAACAC ACTCCCTTCT 
GCAGGTTTTG AAAAGAGCAA TATGGGCATC AGCATAGCTT CGTTTCAGTG GAAAGACATG 
AAGGCTTTAC ATATTAACTA TGTGCAGTCC AGGCATCTGG GGATGGAACC AACGGCTGAC 
AAGTTTGTGT TGTATGTCAC TGATGGGAAG CGACGCTCCA TGGAGATACC ATTTTACATT 
ATAATCAACG CTACAAATGA TGAAGCTCCT GATTTTATAG TGCAGAATAT TACTGTGTGT 
GAGGGTCAGA TGAGAGAGCT GGATTCTTCC ATCATCAGTG CTACTGACCG GGACATTCCC 
AAGAACCCCT TGCTGTTCAG TATCACCCAC AAACCGCGTC ATGGCCTCCT CATCAACAGG 
GCATTTAGCA GAAACTTTTT TCAATATAAG CAACTAGCCA ACCCTGGCCA GAAGCATGAA 
CTTGTCTACA ACTTTTCCAT GGAACTTCTC AAGAATGGAA TGAGGTTGGT GTATGTGCAT 
GATGATTCAG AGAGTCTTGC TGATGACTTT ACAATCCAGG TGTCAGATGG GAAACATAAG 
ATACTTAAAA CCATTTTTGT AGAGGTCACC CCAGTTAATG ATGAGAAACC AATGCTGAGC 
AAGAAGGCTG AAATTACAAT AAATATGGGC GAAACTCGTA TTATTTCTAG TGCTGTTCTC 
TCAGCCATAG ATGAAGACTC ACCCAGGGAG AAGATTTACT ATATATTTGA AAGAATTCCC 
CAAAATGGGC AACTTCAACT TAAGATAGGG AGGGACTGGG TCCCTCTGTA CCCTGGCATG 
AAATGCACTC AGGAAGAAGT GGATTTGAAC TTGCTGAGAT ATACACACAC CGGGGCCATG 
GACTCCAAGA ACCAAGACAG CTTCACCTTC CGCCTCTGGG ACGGTGACAA CAGGTCACCG 
GCATTTGACT GTCACATCAC CATCAAGAAC ATGGGGAAAG GTGATATTGT CATCCTGGCA 
AAACCACTTG TGGTGTCTAA AGGTGACAGA GGTTTCCTGA CGACCACCAC TCTCCTTGCT 
GTGGATGGAA CAGACAAGCC CGAGGAACTT CTCTACGTCA TCACCTCCCC GCCGCAATTT 
GGCCAGGTTG AGTACGTCCG CTATCCTGGG GTCCCCATTA CAAGCTTCAG CCAAATGGAT 
ATAGCAGGAC AGACAGTCTG CTATGTAGAT AAGAGCACGA CAGCTGTCCA CAGAGACACG 
TTCAGATTCA TCGTCAGCAA TGGACTTCAG ACCAAGCCTG GGGTTTTTGA GATCACACTG 
GAGACTGTGG ACACAGCCTT ACCCGTGGTG ACCAGGAACA AGGGGTTGAG ACTGGTTGAA 
GGGGCCACGG GCCTGCTCTC CCCTGACCTC CTGCAGTTGA CTGACCCTGA CACTCCAGCC 
GAGAAGCTCA CCTTTCTTCT GGCCCAGCTC CCACGGCATG GCCAGCTCTA CCTGCGGGGG 
ATGGCGCTGA TCCCACACAA TTTCACTCAG CTGGACGTGG ACAGTGGGCA TGTGGCCTAT 
CGGCACTTGG GAGGGGATTC TTGGACTGAC CACTTCACTT TTGTGGCCAC GGATGGGACG 
AACCATGGCT TTCTTGTGGA TGGGAGAGTG TGGCAAGAAC CTGTATCATT CACCATCCAG 
GTGGACCAGT TGGACAAAGC GGCCCCCCGT GTCACACACT TGCATTCCCC TTCTCAAGTA 
GGGCTCTTGA AAAATGGTTG TTATGGGATT TACATCACCT CCCGTGTGTT GAAGGCATCA 
GACCCCGACA CTGATGATAA TCAGATCATC TTTAAGATTT TACGAGGCCC CCAACATGGG 
CATCTGGAGA ACTCAACGAC AGGTGAATTT ATCCATGAGA AATTTAGCCA GAAGGATTTA 
AACAGTAAGA CCGTTCTTTA CATCATAAAC CCATCTTTGG AAGTAAATTC AGATACCATG 
GAATTTCAAG TCATGGATCC CACAGGGAAC TCTGCCACTC CTCAAAGTTT GGAACTGAAT 
TGGTCTTATA TTGAGTGGTC GCGGACTGAA TATGAGGTCT GTGAGAATAT GGGCATGCTA 
CCCTTGGAGA TTACCAGAAG GGGATATTCC ATGGATTCAG CCTTTGTGAC TGTACAGGTC 
AACCAAGTGT CAGCTACAGT TGGAAAGGAT TTCACCATGA CTCCATCTAA GCTGGTGCAG 
TTTGACCCAG GAATGTCAAC TAAGATGTGG AATATAGCAA TTACCTATGA CGGATTAGAG 
GAAGATGATG AGGTCTTTGA AGTAATTCTG AACTCCCCCG TGAATGCGGT TCTTGGCACA 
AAGACAAAAA CTGCAGTGAA AATTTTGGAC TCAAAAGGAG GGCAGTGCCA TCCTTCAAAT 
GCCTTCAATT CAAACATGCC CAGTGCATGG GAGAAGGGCA TTTGGCACCT GCTCTCCCCA 
GGGGCTTCCT CACCCACCAC TTCTGATTCC TTTCATCTGG AAAGAAGACC CCTTCCAACT 
TCCAAGCAGC TAGCGGTCAC CAGGGGTGAC GCCCTGCGGG ACTTTGGTTC TGAAGATCTT 
TCTCCAGTGA AGCTTAGGAC CCGAGGGGAT GGCAAAACAG TTCCTCCATC CTCTGTTTAT 
AAAAATAGAA CAGACATCAT CTATAACTAT CATGGGGTGA TTTCCCTGAA ACTGGAAGAT 
GACGGCTCCC CAGCTCGCAG AAGGAAGGCC AATGTATCCT TTATTAGTGA GCCCCAAAAG 
ACAACCAAAG TGGCAGAACC GCCTCAAGCC GATAAGGAGG AATCCACAAC CGGCTCACAC 
TTCCCCAGAC AGGACCCGTT GCCCTCATTC CCAAAGAACT GCACACCGGA ATTAAAGGGA 
CTCTTCCACT TTGAAGAAAG CCTCCAAAAA CTGTACAAGT GCGATGGAAT TGCCTGGAGA 
TCCTGGAGCC CCCACACCAA GGAGGTGGAA GATAAATCCT GCCCAGCTGG GTGGCACCAT 
CATTCAGGCT ACTGCCACTC CTTGATCACA GAGCAGAAAG GCACGTGGAC TACAGCTGCC 
CAAGCTTGCA GGGAACATTA CCAAGGCAGC CTTGTAACTG TCATCTCCAG GCAGCACATG 
AGATGGCTTT GGGACATCAG TGGAAGAAAG CCGTTTTGGA TAGGTTTGAA TGACCAAGTG 
CGTGCTGGCC ACTGGGAGTG GATTGGCGGT GAACCTGTGA CCTTCACCAA CTGGAGGAGA 
GGGCCCCCGC AACGTTCAAG GCATGGCAAG GACTGTGTTT TGGTTCAAAG GCCAGGAAAA 
TGGCACACAA AGGACTGTAG GAAGGGAAAA TCTCACAGTT ATGTGTGCTC CAGGAAACTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

FREM1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報