RPTOR cDNA ORF clone, Bos taurus(cattle)

The following RPTOR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPTOR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa216192 NM_001192130.2
Latest version!
Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OBa239162 NM_001192130.3
Latest version!
Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa94004 XM_015458921.1
Latest version!
Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa40069 NM_001192130.1
Latest version!
Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa30234 XM_005221019.3
Latest version!
Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa216192
    Clone ID Related Accession (Same CDS sequence) NM_001192130.2
    Accession Version NM_001192130.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product regulatory-associated protein of mTOR
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02049448.1, DAAA02049444.1, DAAA02049442.1, DAAA02049441.1 and DAAA02049440.1. On Jun 30, 2017 this sequence version replaced NM_001192130.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00120237, SAMN00120238 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179059.2
    CDS758..4765
    Translation

    Target ORF information:

    RefSeq Version NM_001192130.2
    Organism Bos taurus(cattle)
    Definition Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa239162
    Clone ID Related Accession (Same CDS sequence) NM_001192130.3
    Accession Version NM_001192130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-20
    Organism Bos taurus(cattle)
    Product regulatory-associated protein of mTOR
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000019.1 and DV879279.1. On Jun 21, 2018 this sequence version replaced NM_001192130.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.9519.1, SRR5571260.47242.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATT CTCACCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACCGACGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179059.3
    CDS758..4765
    Translation

    Target ORF information:

    RefSeq Version NM_001192130.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulatory associated protein of MTOR complex 1 (RPTOR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATT CTCACCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACCGACGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94004
    Clone ID Related Accession (Same CDS sequence) XM_015458921.1
    Accession Version XM_015458921.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3810bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulatory-associated protein of mTOR isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGC CACTACCGGA TCCTCTGTCG ATGGGTCCTC AGAAAGCTCT GGAAACCATT 
    GGTGCAAATC TACAGAAGCA GTACGAGAAC TGGCAGCCAA GGGCGCGGTA TAAGCAGAGC
    CTTGATCCGA CTGTGGATGA AGTAAAGAAG CTTTGCACGT CTTTACGCCG AAATGCCAAG
    GAGGAGCGGG TCCTCTTCCA CTACAATGGC CATGGGGTGC CCAGGCCCAC CGTTAATGGC
    GAGATCTGGG TCTTCAACAA GAACTACACT CAGTACATCC CTCTGTCTAT ATACGACCTG
    CAGACGTGGA TGGGTAGCCC CTCCATATTC GTCTATGACT GCTCCAATGC TGGCCTCATT
    GTCAAGTCCT TCAAGCAGTT CGCCCTGCAG AGGGAGCAGG AGCTGGAGGT CGCTGCTATT
    AACCCAAACC ACCCACTGGC CCAGATGCCT TTGCCCCCGT CTATGAAGAA CTGCATTCAG
    CTGGCAGCCT GTGAGGCCAA CGAGCTCCTG CCCATGATCC CCGACCTCCC AGCTGACCTG
    TTCACATCAT GCCTCACCAC CCCCATCAAA ATCGCCCTGC GCTGGTTCTG CATGCAGAAA
    TGTGTTAGTC TGGTGCCTGG AGTCACACTG GATTTGATAG AAAAGATTCC CGGGCGGCTG
    AATGACAGGA GGACCCCCCT GGGCGAGCTG AACTGGATCT TCACAGCCAT CACGGACACG
    ATTGCGTGGA ACGTGCTTCC TCGGGATCTC TTCCAAAAGC TCTTCAGACA GGACCTGTTG
    GTGGCTAGTC TGTTTCGAAA TTTTTTATTG GCAGAAAGAA TCATGAGGTC ATATAACTGC
    ACCCCCGTCA GCAGTCCACG CCTACCCCCC ACGTACATGC ACGCCATGTG GCAAGCCTGG
    GACCTGGCCG TGGACATCTG TCTCTCCCAG CTGCCGACCA TCATCGAAGA AGGCACAGCA
    TTTCGGCACA GCCCCTTCTT TGCTGAGCAG CTGACCGCCT TCCAGGTGTG GCTCACCATG
    GGCGTGGAAA ACAGGAACCC ACCTGAGCAG CTTCCTATTG TCTTACAGGT GCTGCTGAGC
    CAAGTGCACC GGCTGAGAGC CCTGGACTTG CTGGGAAGAT TTCTGGACCT GGGCCCCTGG
    GCAGTGAGCC TGGCGCTGTC TGTGGGCATC TTCCCCTATG TGCTAAAGCT GCTCCAGAGC
    TCAGCCAGGG AGCTGCGGCC TCTCCTTGTC TTCATCTGGG CCAAGATCCT TGCCGTGGAC
    AGTTCATGCC AAGCTGACCT GGTGAAGGAC AACGGCCACA AGTACTTCCT CTCGGTCTTG
    GCAGACCCGT ACATGCCAGC TGAGCACCGG ACCATGACGG CCTTCATTCT GGCTGTAATC
    GTCAACAGCT ATAACACGGG GCAGGAAGCC TGCCTCCAGG GAAACCTGAT CGCCATCTGC
    CTGGAGCAGC TCAACGACCC CCACCCCCTG CTGCGCCAGT GGGTGGCCAT CTGCCTGGGC
    CGCATCTGGC AGAACTTTGA CTCGGCCCGG TGGTGCGGGG TGCGGGACAG CGCCCACGAG
    AAGCTCTGCA GCCTCCTCTC CGACCCCATC CCTGAGGTAC GCTGCGCAGC CGTCTTTGCC
    CTCGGCACGT TTGTGGGTAA CTCTGCAGAG AGGACAGACC ACTCCACCAC CATCGACCAC
    AACGTGGCCA TGATGCTGGC CCAGCTCATC AATGACGGCA GCCCTGTGGT CCGGAAGGAG
    CTGGTGGTGG CTCTGAGTCA CCTTGTCGTT CAGTATGAAA GTAACTTCTG CACTGTGGCC
    CTGCAGTTCA TGGAAGAGGA GAAGAACTAC CCACTGCCCT CTCCAGCCAC CACAGAGGGT
    GGGAGTCTGA CCCCCGTGCG GGACAGCCCC TGCACCCCCA GACTGCGTTC CGTGAGCTCT
    TACGGGAATA TCCGCGCCGT CACCACGGCT AGAAACTTAA ACAAGTCTTT GCAAAACCTG
    AGCCTGACGG AAGAAACCGG TGGCTCGGTG GCCTTCTCCC CCGGGAACCT GAGCACCAGC
    AGCAGCGCCA GCAGCACCCT GGGCAGCCCC GAGAACGAGG AGTACATCCT GTCCTTCGAG
    ACCATTGACA AGATGCGCCG CGTCAGCTCC TACTCCTCCC TCAACTCCCT CATCGGAGTT
    TCATTCAATA GTGTTTACAC TCAAATTTGG AGAGTCTTGC TGCATCTGGC TGCGGACCCC
    TATCCAGATG TTTCTGACTT GGCTATGAAA GTGCTCAACA GCATTGCCTA CAAGGCCACA
    GTGAACGCCC GGCCTCAGCG CATCCTCAGC ACCTCCTCCC TCACGCAGTC GGCGCCTGCC
    AGCCCCACCA ACAAAGGCAT CCACATCCAC CAGGCGGGAG GCTCCCCTCC AACATCCAGC
    GCAAGCAGCT CCAGCCTGAC CAACGACGTG GCCAAGCAGC CGGTCAGCCG AGACCTGCCT
    CCAGGCCGGC CGGGCGCCAC AGGCCCCGTG GGGGTACAGT ACACACCCCA CTCGCACCAG
    TTCCCCCGGA CGCGGAAGAT GTTCGATAAA GGCCCAGACC AGACCACTGA TGACCCAGAT
    GATACTGCCG GACACAAAAG TTTCATCTCG GCCACGATGC AGACGGGGTT CTGCGACTGG
    AGCGCGCGGT ACTTTGCCCA GCCTGTCATG AAGATTCCGG AAGAGCATGA CCTGGAGAGT
    CAGATCCGCA AGGAGCGGGA GTGGCGGTTT CTGCGGAACA CCCGTGTCCG GAAGCAGGCA
    CAACAGCTCA TCCAGAAGGG TATCACAAGG CTGGACGACC AGATATTTCT GAACAGGAAC
    CCTGGCGTCC CCTCCGTGGT CAAGTTTCAC CCCTTTACAC CATGCGTGGC CGTCGCTGAC
    AAAGACAGCA TCTGCTTTTG GGACTGGGAG AAAGGGGAGA AGCTGGATTA TTTCCACAAC
    GGAAACCCTC GGTACACGAG GGTCACGGCC ATGGAGTATC TGAATGGCCA GGATTGCTCC
    CTCCTGCTCA CAGCCACAGA TGACGGTGCC ATCAGGGTCT GGAAGAACTT TGCTGATTTG
    GAAAAGAACC CAGAAATGGT GACAGCCTGG CAGGGACTCT CGGACATGCT GCCCACCACC
    CGAGGTCTGG CCCGAAAGGT CTCAATCTTT CTTGACCACA ATAAGCAGGG AGGAGCTGGG
    ATGGTGGTGG ACTGGGAGCA GGAGACCGGC CTCCTCATGA GCTCGGGAGA CGTGCGGATC
    ATCCGGATCT GGGACACAGA CCGAGAGATG AAGGTGCAGG ACATCCCCAC GGGCGCCGAC
    AGCTGCGTGA CGAGCCTCTC CTGCGACTCC CACCGCTCCC TCATTGTGGC CGGGCTCGGC
    GACGGCTCCA TCCGCGTCTA TGACAGGAGG ATGGCGCTCA GCGAATGCCG CGTCATGACC
    TACCGGGAGC ACACGGCCTG GGTGGTGAAG GCCTACCTCC AGAAGCACCC CGAGGGCCAC
    ATCATGAGCG TCAGCGTCAA CGGGGATGTG CGCTTCTTTG ACCCTCGGCT GCCGGAGTCT
    GTGAACGTCA TGCAGATCGT GAAGGGGCTG ACAGCGCTCG ACATCCACCC CCAGGCAAAC
    CTGATTGCGT GTGGCTCCAT GAACCAGTTC ACTGCCATCT ACAACGGCAG CGGAGAGCTG
    ATCAACCATA TCAAGTACTA CGACGGCTTC ATGGGCCAGC GGGTCGGTGC CATCAGCTGC
    CTGGCCTTCC ACCCACACTG GCCGCACCTG GCCGTGGGCA GCAATGACTA CTACATCTCC
    GTGTACTCGG TGGAGAAGCG TGTCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015314407.1
    CDS241..4050
    Translation

    Target ORF information:

    RefSeq Version XM_015458921.1
    Organism Bos taurus(cattle)
    Definition Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015458921.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGATGTGC CACTACCGGA TCCTCTGTCG ATGGGTCCTC AGAAAGCTCT GGAAACCATT 
    GGTGCAAATC TACAGAAGCA GTACGAGAAC TGGCAGCCAA GGGCGCGGTA TAAGCAGAGC
    CTTGATCCGA CTGTGGATGA AGTAAAGAAG CTTTGCACGT CTTTACGCCG AAATGCCAAG
    GAGGAGCGGG TCCTCTTCCA CTACAATGGC CATGGGGTGC CCAGGCCCAC CGTTAATGGC
    GAGATCTGGG TCTTCAACAA GAACTACACT CAGTACATCC CTCTGTCTAT ATACGACCTG
    CAGACGTGGA TGGGTAGCCC CTCCATATTC GTCTATGACT GCTCCAATGC TGGCCTCATT
    GTCAAGTCCT TCAAGCAGTT CGCCCTGCAG AGGGAGCAGG AGCTGGAGGT CGCTGCTATT
    AACCCAAACC ACCCACTGGC CCAGATGCCT TTGCCCCCGT CTATGAAGAA CTGCATTCAG
    CTGGCAGCCT GTGAGGCCAA CGAGCTCCTG CCCATGATCC CCGACCTCCC AGCTGACCTG
    TTCACATCAT GCCTCACCAC CCCCATCAAA ATCGCCCTGC GCTGGTTCTG CATGCAGAAA
    TGTGTTAGTC TGGTGCCTGG AGTCACACTG GATTTGATAG AAAAGATTCC CGGGCGGCTG
    AATGACAGGA GGACCCCCCT GGGCGAGCTG AACTGGATCT TCACAGCCAT CACGGACACG
    ATTGCGTGGA ACGTGCTTCC TCGGGATCTC TTCCAAAAGC TCTTCAGACA GGACCTGTTG
    GTGGCTAGTC TGTTTCGAAA TTTTTTATTG GCAGAAAGAA TCATGAGGTC ATATAACTGC
    ACCCCCGTCA GCAGTCCACG CCTACCCCCC ACGTACATGC ACGCCATGTG GCAAGCCTGG
    GACCTGGCCG TGGACATCTG TCTCTCCCAG CTGCCGACCA TCATCGAAGA AGGCACAGCA
    TTTCGGCACA GCCCCTTCTT TGCTGAGCAG CTGACCGCCT TCCAGGTGTG GCTCACCATG
    GGCGTGGAAA ACAGGAACCC ACCTGAGCAG CTTCCTATTG TCTTACAGGT GCTGCTGAGC
    CAAGTGCACC GGCTGAGAGC CCTGGACTTG CTGGGAAGAT TTCTGGACCT GGGCCCCTGG
    GCAGTGAGCC TGGCGCTGTC TGTGGGCATC TTCCCCTATG TGCTAAAGCT GCTCCAGAGC
    TCAGCCAGGG AGCTGCGGCC TCTCCTTGTC TTCATCTGGG CCAAGATCCT TGCCGTGGAC
    AGTTCATGCC AAGCTGACCT GGTGAAGGAC AACGGCCACA AGTACTTCCT CTCGGTCTTG
    GCAGACCCGT ACATGCCAGC TGAGCACCGG ACCATGACGG CCTTCATTCT GGCTGTAATC
    GTCAACAGCT ATAACACGGG GCAGGAAGCC TGCCTCCAGG GAAACCTGAT CGCCATCTGC
    CTGGAGCAGC TCAACGACCC CCACCCCCTG CTGCGCCAGT GGGTGGCCAT CTGCCTGGGC
    CGCATCTGGC AGAACTTTGA CTCGGCCCGG TGGTGCGGGG TGCGGGACAG CGCCCACGAG
    AAGCTCTGCA GCCTCCTCTC CGACCCCATC CCTGAGGTAC GCTGCGCAGC CGTCTTTGCC
    CTCGGCACGT TTGTGGGTAA CTCTGCAGAG AGGACAGACC ACTCCACCAC CATCGACCAC
    AACGTGGCCA TGATGCTGGC CCAGCTCATC AATGACGGCA GCCCTGTGGT CCGGAAGGAG
    CTGGTGGTGG CTCTGAGTCA CCTTGTCGTT CAGTATGAAA GTAACTTCTG CACTGTGGCC
    CTGCAGTTCA TGGAAGAGGA GAAGAACTAC CCACTGCCCT CTCCAGCCAC CACAGAGGGT
    GGGAGTCTGA CCCCCGTGCG GGACAGCCCC TGCACCCCCA GACTGCGTTC CGTGAGCTCT
    TACGGGAATA TCCGCGCCGT CACCACGGCT AGAAACTTAA ACAAGTCTTT GCAAAACCTG
    AGCCTGACGG AAGAAACCGG TGGCTCGGTG GCCTTCTCCC CCGGGAACCT GAGCACCAGC
    AGCAGCGCCA GCAGCACCCT GGGCAGCCCC GAGAACGAGG AGTACATCCT GTCCTTCGAG
    ACCATTGACA AGATGCGCCG CGTCAGCTCC TACTCCTCCC TCAACTCCCT CATCGGAGTT
    TCATTCAATA GTGTTTACAC TCAAATTTGG AGAGTCTTGC TGCATCTGGC TGCGGACCCC
    TATCCAGATG TTTCTGACTT GGCTATGAAA GTGCTCAACA GCATTGCCTA CAAGGCCACA
    GTGAACGCCC GGCCTCAGCG CATCCTCAGC ACCTCCTCCC TCACGCAGTC GGCGCCTGCC
    AGCCCCACCA ACAAAGGCAT CCACATCCAC CAGGCGGGAG GCTCCCCTCC AACATCCAGC
    GCAAGCAGCT CCAGCCTGAC CAACGACGTG GCCAAGCAGC CGGTCAGCCG AGACCTGCCT
    CCAGGCCGGC CGGGCGCCAC AGGCCCCGTG GGGGTACAGT ACACACCCCA CTCGCACCAG
    TTCCCCCGGA CGCGGAAGAT GTTCGATAAA GGCCCAGACC AGACCACTGA TGACCCAGAT
    GATACTGCCG GACACAAAAG TTTCATCTCG GCCACGATGC AGACGGGGTT CTGCGACTGG
    AGCGCGCGGT ACTTTGCCCA GCCTGTCATG AAGATTCCGG AAGAGCATGA CCTGGAGAGT
    CAGATCCGCA AGGAGCGGGA GTGGCGGTTT CTGCGGAACA CCCGTGTCCG GAAGCAGGCA
    CAACAGCTCA TCCAGAAGGG TATCACAAGG CTGGACGACC AGATATTTCT GAACAGGAAC
    CCTGGCGTCC CCTCCGTGGT CAAGTTTCAC CCCTTTACAC CATGCGTGGC CGTCGCTGAC
    AAAGACAGCA TCTGCTTTTG GGACTGGGAG AAAGGGGAGA AGCTGGATTA TTTCCACAAC
    GGAAACCCTC GGTACACGAG GGTCACGGCC ATGGAGTATC TGAATGGCCA GGATTGCTCC
    CTCCTGCTCA CAGCCACAGA TGACGGTGCC ATCAGGGTCT GGAAGAACTT TGCTGATTTG
    GAAAAGAACC CAGAAATGGT GACAGCCTGG CAGGGACTCT CGGACATGCT GCCCACCACC
    CGAGGTCTGG CCCGAAAGGT CTCAATCTTT CTTGACCACA ATAAGCAGGG AGGAGCTGGG
    ATGGTGGTGG ACTGGGAGCA GGAGACCGGC CTCCTCATGA GCTCGGGAGA CGTGCGGATC
    ATCCGGATCT GGGACACAGA CCGAGAGATG AAGGTGCAGG ACATCCCCAC GGGCGCCGAC
    AGCTGCGTGA CGAGCCTCTC CTGCGACTCC CACCGCTCCC TCATTGTGGC CGGGCTCGGC
    GACGGCTCCA TCCGCGTCTA TGACAGGAGG ATGGCGCTCA GCGAATGCCG CGTCATGACC
    TACCGGGAGC ACACGGCCTG GGTGGTGAAG GCCTACCTCC AGAAGCACCC CGAGGGCCAC
    ATCATGAGCG TCAGCGTCAA CGGGGATGTG CGCTTCTTTG ACCCTCGGCT GCCGGAGTCT
    GTGAACGTCA TGCAGATCGT GAAGGGGCTG ACAGCGCTCG ACATCCACCC CCAGGCAAAC
    CTGATTGCGT GTGGCTCCAT GAACCAGTTC ACTGCCATCT ACAACGGCAG CGGAGAGCTG
    ATCAACCATA TCAAGTACTA CGACGGCTTC ATGGGCCAGC GGGTCGGTGC CATCAGCTGC
    CTGGCCTTCC ACCCACACTG GCCGCACCTG GCCGTGGGCA GCAATGACTA CTACATCTCC
    GTGTACTCGG TGGAGAAGCG TGTCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40069
    Clone ID Related Accession (Same CDS sequence) NM_001192130.1
    Accession Version NM_001192130.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4008bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product regulatory-associated protein of mTOR
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC02062143.1, AAFC03012694.1, AAFC03012693.1, AAFC03012692.1, AAFC03035398.1 and AAFC03035399.1. On Jul 17, 2010 this sequence version replaced XM_583606.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00000728, SAMN00120237 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTGCC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179059.1
    CDS758..4765
    Translation

    Target ORF information:

    RefSeq Version NM_001192130.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192130.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTGCC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CACCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCC
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAGGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GAGCTGGGAT GGTGGTGGAC TGGGAGCAGG AGACCGGCCT CCTCATGAGC
    TCGGGAGACG TGCGGATCAT CCGGATCTGG GACACAGACC GAGAGATGAA GGTGCAGGAC
    ATCCCCACGG GCGCCGACAG CTGCGTGACG AGCCTCTCCT GCGACTCCCA CCGCTCCCTC
    ATTGTGGCCG GGCTCGGCGA CGGCTCCATC CGCGTCTATG ACAGGAGGAT GGCGCTCAGC
    GAATGCCGCG TCATGACCTA CCGGGAGCAC ACGGCCTGGG TGGTGAAGGC CTACCTCCAG
    AAGCACCCCG AGGGCCACAT CATGAGCGTC AGCGTCAACG GGGATGTGCG CTTCTTTGAC
    CCTCGGCTGC CGGAGTCTGT GAACGTCATG CAGATCGTGA AGGGGCTGAC AGCGCTCGAC
    ATCCACCCCC AGGCAAACCT GATTGCGTGT GGCTCCATGA ACCAGTTCAC TGCCATCTAC
    AACGGCAGCG GAGAGCTGAT CAACCATATC AAGTACTACG ACGGCTTCAT GGGCCAGCGG
    GTCGGTGCCA TCAGCTGCCT GGCCTTCCAC CCACACTGGC CGCACCTGGC CGTGGGCAGC
    AATGACTACT ACATCTCCGT GTACTCGGTG GAGAAGCGTG TCAGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa30234
    Clone ID Related Accession (Same CDS sequence) XM_005221019.3
    Accession Version XM_005221019.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product regulatory-associated protein of mTOR isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104498.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005221019.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GTCTGGCCCG AAAGGTCTCA ATCTTTCTTG ACCACAATAA GCAGGGAGGA
    GCTGGGATGG TGGTGGACTG GGAGCAGGAG ACCGGCCTCC TCATGAGCTC GGGAGACGTG
    CGGATCATCC GGATCTGGGA CACAGACCGA GAGATGAAGG TGCAGGACAT CCCCACGGGC
    GCCGACAGCT GCGTGACGAG CCTCTCCTGC GACTCCCACC GCTCCCTCAT TGTGGCCGGG
    CTCGGCGACG GCTCCATCCG CGTCTATGAC AGGAGGATGG CGCTCAGCGA ATGCCGCGTC
    ATGACCTACC GGGAGCACAC GGCCTGGGTG GTGAAGGCCT ACCTCCAGAA GCACCCCGAG
    GGCCACATCA TGAGCGTCAG CGTCAACGGG GATGTGCGCT TCTTTGACCC TCGGCTGCCG
    GAGTCTGTGA ACGTCATGCA GATCGTGAAG GGGCTGACAG CGCTCGACAT CCACCCCCAG
    GCAAACCTGA TTGCGTGTGG CTCCATGAAC CAGTTCACTG CCATCTACAA CGGCAGCGGA
    GAGCTGATCA ACCATATCAA GTACTACGAC GGCTTCATGG GCCAGCGGGT CGGTGCCATC
    AGCTGCCTGG CCTTCCACCC ACACTGGCCG CACCTGGCCG TGGGCAGCAA TGACTACTAC
    ATCTCCGTGT ACTCGGTGGA GAAGCGTGTC AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005221076.1
    CDS1213..5268
    Translation

    Target ORF information:

    RefSeq Version XM_005221019.3
    Organism Bos taurus(cattle)
    Definition Bos taurus regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005221019.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGAGTCCG AGATGCTGCA GTCGCCTCTC TTGGGACTCG GGGAGGAAGA TGAAGCTGAC 
    CTTACAGACT GGAACCTACC TTTGGCTTTT ATGAAAAAGA GGCACTGTGA GAAAATCGAA
    GGCTCCAAAT CCTTAGCTCA GAGCTGGAGG ATGAAGGATC GGATGAAGAC CGTCAGTGTT
    GCCTTAGTTT TGTGCCTGAA CGTCGGGGTG GACCCTCCAG ATGTGGTCAA GACCACCCCT
    TGTGCGCGTT TGGAATGCTG GATTGATCCT CTGTCGATGG GTCCTCAGAA AGCTCTGGAA
    ACCATTGGTG CAAATCTACA GAAGCAGTAC GAGAACTGGC AGCCAAGGGC GCGGTATAAG
    CAGAGCCTTG ATCCGACTGT GGATGAAGTA AAGAAGCTTT GCACGTCTTT ACGCCGAAAT
    GCCAAGGAGG AGCGGGTCCT CTTCCACTAC AATGGCCATG GGGTGCCCAG GCCCACCGTT
    AATGGCGAGA TCTGGGTCTT CAACAAGAAC TACACTCAGT ACATCCCTCT GTCTATATAC
    GACCTGCAGA CGTGGATGGG TAGCCCCTCC ATATTCGTCT ATGACTGCTC CAATGCTGGC
    CTCATTGTCA AGTCCTTCAA GCAGTTCGCC CTGCAGAGGG AGCAGGAGCT GGAGGTCGCT
    GCTATTAACC CAAACCACCC ACTGGCCCAG ATGCCTTTGC CCCCGTCTAT GAAGAACTGC
    ATTCAGCTGG CAGCCTGTGA GGCCAACGAG CTCCTGCCCA TGATCCCCGA CCTCCCAGCT
    GACCTGTTCA CATCATGCCT CACCACCCCC ATCAAAATCG CCCTGCGCTG GTTCTGCATG
    CAGAAATGTG TTAGTCTGGT GCCTGGAGTC ACACTGGATT TGATAGAAAA GATTCCCGGG
    CGGCTGAATG ACAGGAGGAC CCCCCTGGGC GAGCTGAACT GGATCTTCAC AGCCATCACG
    GACACGATTG CGTGGAACGT GCTTCCTCGG GATCTCTTCC AAAAGCTCTT CAGACAGGAC
    CTGTTGGTGG CTAGTCTGTT TCGAAATTTT TTATTGGCAG AAAGAATCAT GAGGTCATAT
    AACTGCACCC CCGTCAGCAG TCCACGCCTA CCCCCCACGT ACATGCACGC CATGTGGCAA
    GCCTGGGACC TGGCCGTGGA CATCTGTCTC TCCCAGCTGC CGACCATCAT CGAAGAAGGC
    ACAGCATTTC GGCACAGCCC CTTCTTTGCT GAGCAGCTGA CCGCCTTCCA GGTGTGGCTC
    ACCATGGGCG TGGAAAACAG GAACCCACCT GAGCAGCTTC CTATTGTCTT ACAGGTGCTG
    CTGAGCCAAG TGCACCGGCT GAGAGCCCTG GACTTGCTGG GAAGATTTCT GGACCTGGGC
    CCCTGGGCAG TGAGCCTGGC GCTGTCTGTG GGCATCTTCC CCTATGTGCT AAAGCTGCTC
    CAGAGCTCAG CCAGGGAGCT GCGGCCTCTC CTTGTCTTCA TCTGGGCCAA GATCCTTGCC
    GTGGACAGTT CATGCCAAGC TGACCTGGTG AAGGACAACG GCCACAAGTA CTTCCTCTCG
    GTCTTGGCAG ACCCGTACAT GCCAGCTGAG CACCGGACCA TGACGGCCTT CATTCTGGCT
    GTAATCGTCA ACAGCTATAA CACGGGGCAG GAAGCCTGCC TCCAGGGAAA CCTGATCGCC
    ATCTGCCTGG AGCAGCTCAA CGACCCCCAC CCCCTGCTGC GCCAGTGGGT GGCCATCTGC
    CTGGGCCGCA TCTGGCAGAA CTTTGACTCG GCCCGGTGGT GCGGGGTGCG GGACAGCGCC
    CACGAGAAGC TCTGCAGCCT CCTCTCCGAC CCCATCCCTG AGGTACGCTG CGCAGCCGTC
    TTTGCCCTCG GCACGTTTGT GGGTAACTCT GCAGAGAGGA CAGACCACTC CACCACCATC
    GACCACAACG TGGCCATGAT GCTGGCCCAG CTCATCAATG ACGGCAGCCC TGTGGTCCGG
    AAGGAGCTGG TGGTGGCTCT GAGTCACCTT GTCGTTCAGT ATGAAAGTAA CTTCTGCACT
    GTGGCCCTGC AGTTCATGGA AGAGGAGAAG AACTACCCAC TGCCCTCTCC AGCCACCACA
    GAGGGTGGGA GTCTGACCCC CGTGCGGGAC AGCCCCTGCA CCCCCAGACT GCGTTCCGTG
    AGCTCTTACG GGAATATCCG CGCCGTCACC ACGGCTAGAA ACTTAAACAA GTCTTTGCAA
    AACCTGAGCC TGACGGAAGA AACCGGTGGC TCGGTGGCCT TCTCCCCCGG GAACCTGAGC
    ACCAGCAGCA GCGCCAGCAG CACCCTGGGC AGCCCCGAGA ACGAGGAGTA CATCCTGTCC
    TTCGAGACCA TTGACAAGAT GCGCCGCGTC AGCTCCTACT CCTCCCTCAA CTCCCTCATC
    GGAGTTTCAT TCAATAGTGT TTACACTCAA ATTTGGAGAG TCTTGCTGCA TCTGGCTGCG
    GACCCCTATC CAGATGTTTC TGACTTGGCT ATGAAAGTGC TCAACAGCAT TGCCTACAAG
    GCCACAGTGA ACGCCCGGCC TCAGCGCATC CTCAGCACCT CCTCCCTCAC GCAGTCGGCG
    CCTGCCAGCC CCACCAACAA AGGCATCCAC ATCCACCAGG CGGGAGGCTC CCCTCCAACA
    TCCAGCGCAA GCAGCTCCAG CCTGACCAAC GACGTGGCCA AGCAGCCGGT CAGCCGAGAC
    CTGCCTCCAG GCCGGCCGGG CGCCACAGGC CCCGTGGGGG TACAGTACAC ACCCCACTCG
    CACCAGTTCC CCCGGACGCG GAAGATGTTC GATAAAGGCC CAGACCAGAC CACTGATGAC
    CCAGATGATA CTGCCGGACA CAAAAGTTTC ATCTCGGCCA CGATGCAGAC GGGGTTCTGC
    GACTGGAGCG CGCGGTACTT TGCCCAGCCT GTCATGAAGA TTCCGGAAGA GCATGACCTG
    GAGAGTCAGA TCCGCAAGGA GCGGGAGTGG CGGTTTCTGC GGAACACCCG TGTCCGGAAG
    CAGGCACAAC AGCTCATCCA GAAGGGTATC ACAAGGCTGG ACGACCAGAT ATTTCTGAAC
    AGGAACCCTG GCGTCCCCTC CGTGGTCAAG TTTCACCCCT TTACACCATG CGTGGCCGTC
    GCTGACAAAG ACAGCATCTG CTTTTGGGAC TGGGAGAAAG GGGAGAAGCT GGATTATTTC
    CACAACGGAA ACCCTCGGTA CACGAGGGTC ACGGCCATGG AGTATCTGAA TGGCCAGGAT
    TGCTCCCTCC TGCTCACAGC CACAGATGAC GGTGCCATCA GGGTCTGGAA GAACTTTGCT
    GATTTGGAAA AGAACCCAGA AATGGTGACA GCCTGGCAGG GACTCTCGGA CATGCTGCCC
    ACCACCCGAG GTCTGGCCCG AAAGGTCTCA ATCTTTCTTG ACCACAATAA GCAGGGAGGA
    GCTGGGATGG TGGTGGACTG GGAGCAGGAG ACCGGCCTCC TCATGAGCTC GGGAGACGTG
    CGGATCATCC GGATCTGGGA CACAGACCGA GAGATGAAGG TGCAGGACAT CCCCACGGGC
    GCCGACAGCT GCGTGACGAG CCTCTCCTGC GACTCCCACC GCTCCCTCAT TGTGGCCGGG
    CTCGGCGACG GCTCCATCCG CGTCTATGAC AGGAGGATGG CGCTCAGCGA ATGCCGCGTC
    ATGACCTACC GGGAGCACAC GGCCTGGGTG GTGAAGGCCT ACCTCCAGAA GCACCCCGAG
    GGCCACATCA TGAGCGTCAG CGTCAACGGG GATGTGCGCT TCTTTGACCC TCGGCTGCCG
    GAGTCTGTGA ACGTCATGCA GATCGTGAAG GGGCTGACAG CGCTCGACAT CCACCCCCAG
    GCAAACCTGA TTGCGTGTGG CTCCATGAAC CAGTTCACTG CCATCTACAA CGGCAGCGGA
    GAGCTGATCA ACCATATCAA GTACTACGAC GGCTTCATGG GCCAGCGGGT CGGTGCCATC
    AGCTGCCTGG CCTTCCACCC ACACTGGCCG CACCTGGCCG TGGGCAGCAA TGACTACTAC
    ATCTCCGTGT ACTCGGTGGA GAAGCGTGTC AGATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.