TTC3 cDNA ORF clone, Bos taurus(cattle)

The following TTC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TTC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa218662 XM_025000044.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa218653 XM_005202057.4
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218653 XM_005202058.4
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218653 XM_005202056.4
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218654 XM_010801674.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218655 XM_010801675.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218656 XM_024999804.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218657 XM_024999841.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218658 XM_010801678.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218659 XM_010801679.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218660 XM_024999925.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218661 XM_005202061.4
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218661 XM_005202060.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa218661 XM_024999992.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa16127 NM_001109767.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16127 XM_005202057.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16127 XM_005202056.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16127 XM_005202058.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16129 XM_005202060.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa16129 XM_005202061.3
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa46601 XM_010801674.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa46602 XM_010801675.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa46604 XM_010801677.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa46605 XM_010801678.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa46606 XM_010801679.2
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa218652 XM_024999711.1
Latest version!
Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa218662
    Clone ID Related Accession (Same CDS sequence) XM_025000044.1
    Accession Version XM_025000044.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATAAAAT TGACAAGGAT TTTCTGCAAG GAATCTGTCT TACTCCTGAT TGTGAAGGAA 
    TCATTTCAAA GATTATCATC TTCAGCAGTG GTGGTCAAGT TAAATGTGAA CCTAGAGAAG
    TTGAGGCTGA AAGAAGACAA AAAATTGAAG CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA
    TTAGCACAAG AAAGACTGGA AGAGGACTTA AGGGAAAGTA ACCCACCAAA AACTGAAGAG
    CAGAGAGAAA CTATCAACCA TGTCCAAAGT TGCCAGTTCC TTGATGACAG AATTCTGCAG
    TGCATAAAGC AGTACGCTGA CAAGATTAAG TCCGGGATAC TGAATACTTC CAAGCTTCTC
    AAAGAATTGC TTTCTTGGAA GGTTTTAACC ACAGAAGACT ACACAACGTG TTTTTCTAGC
    AGAAATTTCC TGAATGAAGC AGTGGACTAT GTCATTCGTC ATTTGATCCA AGAAAAGAAT
    AGAGTAAAGA CAAGGGTATT TCTGCATGTT TTGAGTGAGC TTAAAGAAGT GGACCCAAAA
    CTAGCCACCT GGATCCAGAA ACTGAATAGC TTTGGCTTAG ATGCCACAGG ACCTTTCTTT
    TCTCGTTATG GGGCTCCTCT TAAGGAGCTT GATTTTAGCA TCATGACTTT TCTCTGGAAT
    GAGAAATACG GTCATAAGCT AGGCTCTATA GAAGGAAAGC AACTTGATTA TTTCTATGAG
    CCTACATCAC TGAAGGAAGC GCGGTGTTTA ATATGGCTGC TAGAAGAACA CAGAGACAAG
    TTCCCAGCAC TGCATAATGC CTTGGACGAG TTCTTTGATA TAATGGACAG CCGCTGCACT
    GTATTAAGGA AACAGGACAG TGGTGACGTG CCATTTAATT CTACCAAGAT AAAAAACAAA
    GGCAAGAAAA AGAAGCCAAA AGATTCAAAG CCTATGTTAG TTGGATCTGG AACACCTTCA
    GTCACACCAA ACAATGAGAC TGTCACTTCA GGTGAAGACC ATATCAAACG CAGTTTAAGT
    TCTGCGGGCC CCTTTGTAGT GCCTGATCAC CTTCGGCAGG ATATAGAAGA ATTTGAAGCT
    CTGTATGAAC AGCATAGTAA TGAGTATGTT GTCCGTAATA AGAAGCTGTG GGACATAAAC
    CCAAAACAGA AATGTTCAAC TCTATACGAT TATTTCTCTC AGTTGTTGGA GGAACATGGT
    CCCTTGGACA TGAGTAACAA AATGTTTTCT GAAGAATATG AGTTCTTCCC AGAAGAAACT
    CAACAGATAC TAGAAAAAGC AGGAGGCTTG AAAGCGTTTC TCCTGGGATG TCCTCGTTTT
    GTTGTGATTG ATAACTGTAT CGCATTGAAG AAAGTTGCAT TACGGCTCAA GAAAAAGAGA
    AAGAAGAAAA ACATTAAAAC AAAAGTAGAA GAAATTTCAA AAACAGGAGA GTATTTACGA
    GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA
    GTGTCTGACT TACCTTCAGC ACCAGCTTCT GAGGACATGA AGCCTCAGCC TGCACCTGCT
    AATTCTCCCC AATCAGCTCG TGACACTGGG AAGCCAAAAC CAGTTTTCGA TAATTCTTCC
    AGATCAGTTT CTGAGGATGA GAAGCCCAAA GGGATGTCTT CTGATTCTCC CAGGCCAGTC
    TTTGAGGATA CCGGTCACAA GCCGGTCTCC TCTAATTCTC CCAAACCAGT TCCTGAGGAA
    GTGAAAGCGA CCTATTGGCC TCGACCCCAG TTGGTCACAG GATACTGTAC ATATCTTCCC
    TTTCGGGGAT TTGACATTAC CCAGACTCCA CCAGCCTTCA TCAGTGTGTT ACCGACTTTG
    CCCCAGTATA GCATTTACAC CCCTCTGGCC AGTGTTCCTA CTGAGTATCA CCTGCAAAGA
    CCAATACCAG TGGTGCCGTC TTTTGTAGCC AGTGACATAA CGGAAGTGCA TTTTGAGGCT
    CATCATTTCA GTGCTGAGAA TGCCCCTGGT ACCCAGATTG CCTCCGAAAC ACAGATCCTT
    GAGGAATCTT TGGAAACACC AGTGAAGTCA GAGTGCAGCT ATGATGGTGC TGATATAGTC
    CAGAGTGAGG CTAGCAGAAA CGATGACTAC TGTGGCGATT CTGCCAAATG GGAAGTAAAT
    CCAGAAAGCA CCAATGAAGT AACAAATATT CCATGTACAC AAGTGGTTGC TGTACAGGTG
    TCTTGGAATA TAACACATCA GGAAGTCAAT ACTGAGCCAT ATGGTCCTTT TGAGGCACAA
    CAGGGGGGTA TTTCACAGGT TGAAAAGGAG TACCAGGTTT TACAAGAGCA GCTTAAGGAA
    GCATGTGAAA ATTATGAACA CAGCAATGTC AAGGGCTCAG AAGAAACCAA GGACTTGGAA
    GAAAAGTTGA AAAGGAATGT TGAAGAAAAT AAGATCTCAA AGACAGAACT AGATTGGTTC
    CTTGAAGATT TGAACAAGGA AATTAAAAAA TGGGAACAGG AGAAAAAAGA AATCCAAGAA
    AGACTAAAGG CACTGAAGAA GAAAATGAAA AAGGTGTTAA ATGCCAGCAA AATGTATACG
    CAGGAAAATG ATGGAAAGGA AAAGGCACTT GAATTACTTC TGGATCAGTC CCTTGAAATC
    AGTAATACAT TTACAAATGA GAAAATGAAA ATAGAAGAGA GTATAAAGAA AGGGAAAGAG
    CATTATGAAG AGAGTCTTCA AAGGGCTGTG GATGCAGAGG TATCTGTGCT TGAAAACTGG
    AAGGACATGG AAGTGTGTAA GTTACAGAGC ATGGAGTCAC AAGCCGAAAG GTTTCTCAAG
    AATCTGAAGC TAATGAGCAG TGATTCGGCA GCGTATTCTG ACATGGACTC AGACATACAT
    TCATGGGAAT CGTTTCTTTC TGATGTTAGA GAAGAAATTG AGAAAGCAAA GTCTCAGTTT
    GAAGAACAAA TTCAGGCAAT TAAATATGGT TCTCAGCTCA GTGAACTTCC TAAAGTGCAG
    ATTTCTGAGC TTTCATTTCC TACCTGTAAC ACGATTCATC CTAAATTACT TCCTGAGTCT
    TCAGGCCAGG ATGGTCAAGG TCCCTCCACT TCTACAAGTC ATGGGACCGG AGACCAGACA
    GCAGCTCCCG AGGAGTCAAG TCTGGGCTCT GCAGCTGACA GCCCAGTGGA GTTGCCCTCC
    TGCAGTCAGA AAGCCCCCCA GCTGCCAGAG CATGAGGGTG CTGGCCAGGC AACTGGGCCA
    GAACCAGGCC CAGTGGCTGA TCAGAAGTTA CCTGCTGCTC CAGAGCGTGC CACTCATTCA
    AGCCAGTCTC CAAAGAAGCC GTTCAATAGC ATTATTGAGC ACCTGTTGGT GGTGTTCCCG
    TGTTACAGCA GCACGGAGCT TGCTGGTTTT ATTAAAAAAG TTAGAAACAA AAACAAGACC
    TCTCTGTCTG GATTGAGTAT TGACGAAATT GTGCAAAGAG TGACAGAACA CGTCCTTGAT
    GAAGAAAGAA AGAAAAAGCC AAGTCCAGGA AAGGGCTGGA GGCTGTCTGC ACCCAGCTCT
    GCTGTTGCTG TGCCCAGGGC TGCCCAGGAC CCGCCCTCCA TGCCTGTCGG AGCATCGCCC
    GAAACCAGGG GGCCGAGGAC CGAGGACGTC CCTGTGTCCT GCGCAAATTC CTGTGAAATG
    TGCCGAGAAG TATTCAAGTC CAAACACGTG CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC
    AAGGGGTGCT TCAAGCAGTG GCTGAAGAGC CAGCGCACCT GCCCAGCCTG CCTGGGTCAC
    GACCTCCTGT CAGAGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855812.1
    CDS521..4321
    Translation

    Target ORF information:

    RefSeq Version XM_025000044.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025000044.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGATAAAAT TGACAAGGAT TTTCTGCAAG GAATCTGTCT TACTCCTGAT TGTGAAGGAA 
    TCATTTCAAA GATTATCATC TTCAGCAGTG GTGGTCAAGT TAAATGTGAA CCTAGAGAAG
    TTGAGGCTGA AAGAAGACAA AAAATTGAAG CGAAAGCTCC AAAAAAAGGA AGCAAAAAAA
    TTAGCACAAG AAAGACTGGA AGAGGACTTA AGGGAAAGTA ACCCACCAAA AACTGAAGAG
    CAGAGAGAAA CTATCAACCA TGTCCAAAGT TGCCAGTTCC TTGATGACAG AATTCTGCAG
    TGCATAAAGC AGTACGCTGA CAAGATTAAG TCCGGGATAC TGAATACTTC CAAGCTTCTC
    AAAGAATTGC TTTCTTGGAA GGTTTTAACC ACAGAAGACT ACACAACGTG TTTTTCTAGC
    AGAAATTTCC TGAATGAAGC AGTGGACTAT GTCATTCGTC ATTTGATCCA AGAAAAGAAT
    AGAGTAAAGA CAAGGGTATT TCTGCATGTT TTGAGTGAGC TTAAAGAAGT GGACCCAAAA
    CTAGCCACCT GGATCCAGAA ACTGAATAGC TTTGGCTTAG ATGCCACAGG ACCTTTCTTT
    TCTCGTTATG GGGCTCCTCT TAAGGAGCTT GATTTTAGCA TCATGACTTT TCTCTGGAAT
    GAGAAATACG GTCATAAGCT AGGCTCTATA GAAGGAAAGC AACTTGATTA TTTCTATGAG
    CCTACATCAC TGAAGGAAGC GCGGTGTTTA ATATGGCTGC TAGAAGAACA CAGAGACAAG
    TTCCCAGCAC TGCATAATGC CTTGGACGAG TTCTTTGATA TAATGGACAG CCGCTGCACT
    GTATTAAGGA AACAGGACAG TGGTGACGTG CCATTTAATT CTACCAAGAT AAAAAACAAA
    GGCAAGAAAA AGAAGCCAAA AGATTCAAAG CCTATGTTAG TTGGATCTGG AACACCTTCA
    GTCACACCAA ACAATGAGAC TGTCACTTCA GGTGAAGACC ATATCAAACG CAGTTTAAGT
    TCTGCGGGCC CCTTTGTAGT GCCTGATCAC CTTCGGCAGG ATATAGAAGA ATTTGAAGCT
    CTGTATGAAC AGCATAGTAA TGAGTATGTT GTCCGTAATA AGAAGCTGTG GGACATAAAC
    CCAAAACAGA AATGTTCAAC TCTATACGAT TATTTCTCTC AGTTGTTGGA GGAACATGGT
    CCCTTGGACA TGAGTAACAA AATGTTTTCT GAAGAATATG AGTTCTTCCC AGAAGAAACT
    CAACAGATAC TAGAAAAAGC AGGAGGCTTG AAAGCGTTTC TCCTGGGATG TCCTCGTTTT
    GTTGTGATTG ATAACTGTAT CGCATTGAAG AAAGTTGCAT TACGGCTCAA GAAAAAGAGA
    AAGAAGAAAA ACATTAAAAC AAAAGTAGAA GAAATTTCAA AAACAGGAGA GTATTTACGA
    GTTAAACTAC CACTGAATCC AACTGCTAGG GAATTTAAAC CAGATGTAAA GTCCAAACCA
    GTGTCTGACT TACCTTCAGC ACCAGCTTCT GAGGACATGA AGCCTCAGCC TGCACCTGCT
    AATTCTCCCC AATCAGCTCG TGACACTGGG AAGCCAAAAC CAGTTTTCGA TAATTCTTCC
    AGATCAGTTT CTGAGGATGA GAAGCCCAAA GGGATGTCTT CTGATTCTCC CAGGCCAGTC
    TTTGAGGATA CCGGTCACAA GCCGGTCTCC TCTAATTCTC CCAAACCAGT TCCTGAGGAA
    GTGAAAGCGA CCTATTGGCC TCGACCCCAG TTGGTCACAG GATACTGTAC ATATCTTCCC
    TTTCGGGGAT TTGACATTAC CCAGACTCCA CCAGCCTTCA TCAGTGTGTT ACCGACTTTG
    CCCCAGTATA GCATTTACAC CCCTCTGGCC AGTGTTCCTA CTGAGTATCA CCTGCAAAGA
    CCAATACCAG TGGTGCCGTC TTTTGTAGCC AGTGACATAA CGGAAGTGCA TTTTGAGGCT
    CATCATTTCA GTGCTGAGAA TGCCCCTGGT ACCCAGATTG CCTCCGAAAC ACAGATCCTT
    GAGGAATCTT TGGAAACACC AGTGAAGTCA GAGTGCAGCT ATGATGGTGC TGATATAGTC
    CAGAGTGAGG CTAGCAGAAA CGATGACTAC TGTGGCGATT CTGCCAAATG GGAAGTAAAT
    CCAGAAAGCA CCAATGAAGT AACAAATATT CCATGTACAC AAGTGGTTGC TGTACAGGTG
    TCTTGGAATA TAACACATCA GGAAGTCAAT ACTGAGCCAT ATGGTCCTTT TGAGGCACAA
    CAGGGGGGTA TTTCACAGGT TGAAAAGGAG TACCAGGTTT TACAAGAGCA GCTTAAGGAA
    GCATGTGAAA ATTATGAACA CAGCAATGTC AAGGGCTCAG AAGAAACCAA GGACTTGGAA
    GAAAAGTTGA AAAGGAATGT TGAAGAAAAT AAGATCTCAA AGACAGAACT AGATTGGTTC
    CTTGAAGATT TGAACAAGGA AATTAAAAAA TGGGAACAGG AGAAAAAAGA AATCCAAGAA
    AGACTAAAGG CACTGAAGAA GAAAATGAAA AAGGTGTTAA ATGCCAGCAA AATGTATACG
    CAGGAAAATG ATGGAAAGGA AAAGGCACTT GAATTACTTC TGGATCAGTC CCTTGAAATC
    AGTAATACAT TTACAAATGA GAAAATGAAA ATAGAAGAGA GTATAAAGAA AGGGAAAGAG
    CATTATGAAG AGAGTCTTCA AAGGGCTGTG GATGCAGAGG TATCTGTGCT TGAAAACTGG
    AAGGACATGG AAGTGTGTAA GTTACAGAGC ATGGAGTCAC AAGCCGAAAG GTTTCTCAAG
    AATCTGAAGC TAATGAGCAG TGATTCGGCA GCGTATTCTG ACATGGACTC AGACATACAT
    TCATGGGAAT CGTTTCTTTC TGATGTTAGA GAAGAAATTG AGAAAGCAAA GTCTCAGTTT
    GAAGAACAAA TTCAGGCAAT TAAATATGGT TCTCAGCTCA GTGAACTTCC TAAAGTGCAG
    ATTTCTGAGC TTTCATTTCC TACCTGTAAC ACGATTCATC CTAAATTACT TCCTGAGTCT
    TCAGGCCAGG ATGGTCAAGG TCCCTCCACT TCTACAAGTC ATGGGACCGG AGACCAGACA
    GCAGCTCCCG AGGAGTCAAG TCTGGGCTCT GCAGCTGACA GCCCAGTGGA GTTGCCCTCC
    TGCAGTCAGA AAGCCCCCCA GCTGCCAGAG CATGAGGGTG CTGGCCAGGC AACTGGGCCA
    GAACCAGGCC CAGTGGCTGA TCAGAAGTTA CCTGCTGCTC CAGAGCGTGC CACTCATTCA
    AGCCAGTCTC CAAAGAAGCC GTTCAATAGC ATTATTGAGC ACCTGTTGGT GGTGTTCCCG
    TGTTACAGCA GCACGGAGCT TGCTGGTTTT ATTAAAAAAG TTAGAAACAA AAACAAGACC
    TCTCTGTCTG GATTGAGTAT TGACGAAATT GTGCAAAGAG TGACAGAACA CGTCCTTGAT
    GAAGAAAGAA AGAAAAAGCC AAGTCCAGGA AAGGGCTGGA GGCTGTCTGC ACCCAGCTCT
    GCTGTTGCTG TGCCCAGGGC TGCCCAGGAC CCGCCCTCCA TGCCTGTCGG AGCATCGCCC
    GAAACCAGGG GGCCGAGGAC CGAGGACGTC CCTGTGTCCT GCGCAAATTC CTGTGAAATG
    TGCCGAGAAG TATTCAAGTC CAAACACGTG CGTGAGCTGC AGTGTGGGCA CAGGCTTCAC
    AAGGGGTGCT TCAAGCAGTG GCTGAAGAGC CAGCGCACCT GCCCAGCCTG CCTGGGTCAC
    GACCTCCTGT CAGAGGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218653
    Clone ID Related Accession (Same CDS sequence) XM_005202057.4 , XM_005202056.4 , XM_005202058.4
    Accession Version XM_005202057.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202057.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202114.1
    CDS1978..7935
    Translation

    Target ORF information:

    RefSeq Version XM_005202057.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202057.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218653
    Clone ID Related Accession (Same CDS sequence) XM_005202057.4 , XM_005202056.4 , XM_005202058.4
    Accession Version XM_005202056.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202056.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202113.1
    CDS255..6212
    Translation

    Target ORF information:

    RefSeq Version XM_005202056.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202056.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218653
    Clone ID Related Accession (Same CDS sequence) XM_005202057.4 , XM_005202056.4 , XM_005202058.4
    Accession Version XM_005202058.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202115.1
    CDS124..6081
    Translation

    Target ORF information:

    RefSeq Version XM_005202058.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202058.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGTT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTAAACCT GGCCATGATT AACTACGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GTCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218654
    Clone ID Related Accession (Same CDS sequence) XM_010801674.2
    Accession Version XM_010801674.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5922bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801674.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT
    GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC
    AGGGGGCCGA GGACCGAGGA CGTCCCTTGC TTCAAGCAGT GGCTGAAGAG CCAGCGCACC
    TGCCCAGCCT GCCTGGGTCA CGACCTCCTG TCAGAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799976.2
    CDS2833..8754
    Translation

    Target ORF information:

    RefSeq Version XM_010801674.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801674.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT
    GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC
    AGGGGGCCGA GGACCGAGGA CGTCCCTTGC TTCAAGCAGT GGCTGAAGAG CCAGCGCACC
    TGCCCAGCCT GCCTGGGTCA CGACCTCCTG TCAGAGGAGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218655
    Clone ID Related Accession (Same CDS sequence) XM_010801675.2
    Accession Version XM_010801675.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5880bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGGTGTCCTG CGCAAATTCC TGTGAAATGT GCCGAGAAGT ATTCAAGTCC
    AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AGGGGTGCTT CAAGCAGTGG
    CTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799977.2
    CDS2832..8711
    Translation

    Target ORF information:

    RefSeq Version XM_010801675.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801675.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGGTGTCCTG CGCAAATTCC TGTGAAATGT GCCGAGAAGT ATTCAAGTCC
    AAACACGTGC GTGAGCTGCA GTGTGGGCAC AGGCTTCACA AGGGGTGCTT CAAGCAGTGG
    CTGAAGAGCC AGCGCACCTG CCCAGCCTGC CTGGGTCACG ACCTCCTGTC AGAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218656
    Clone ID Related Accession (Same CDS sequence) XM_024999804.1
    Accession Version XM_024999804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5787bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGTGCTTCAA GCAGTGGCTG AAGAGCCAGC GCACCTGCCC AGCCTGCCTG
    GGTCACGACC TCCTGTCAGA GGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855572.1
    CDS2833..8619
    Translation

    Target ORF information:

    RefSeq Version XM_024999804.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGTGCTTCAA GCAGTGGCTG AAGAGCCAGC GCACCTGCCC AGCCTGCCTG
    GGTCACGACC TCCTGTCAGA GGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218657
    Clone ID Related Accession (Same CDS sequence) XM_024999841.1
    Accession Version XM_024999841.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
    CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA
    TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG
    GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA
    GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA
    ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG
    GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA
    AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC
    CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT
    GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT
    TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT
    CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG
    TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC
    TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT
    AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT
    AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT
    GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC
    AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC
    ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA
    ATGAAAATGT TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC
    CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG
    CAATTAAACC TGGCCATGAT TAACTACGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC
    CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT
    GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT
    TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC
    TGTCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG
    CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG
    CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA
    ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA
    TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG
    GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC
    ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG
    GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA
    GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA
    AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT
    ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG
    TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT
    TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG
    AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA
    AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG
    ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG
    GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT
    CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG
    AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG
    CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA
    CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG
    AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC
    AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC
    TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG
    CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA
    TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG
    AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA
    GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT
    AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC
    ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA
    CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA
    CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA
    TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT
    GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC
    GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC
    TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT
    GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC
    ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG
    GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT
    GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG
    GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT
    AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC
    AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA
    ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT
    TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT
    TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA
    AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG
    AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA
    CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT
    GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT
    ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG
    AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA
    GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA
    ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG
    TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT
    CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT
    TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT
    GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG
    GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA
    GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA
    GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA
    AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC
    ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA
    TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG
    AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG
    CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG
    CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA
    TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC
    AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA
    GAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855609.1
    CDS455..6103
    Translation

    Target ORF information:

    RefSeq Version XM_024999841.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999841.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
    CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA
    TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG
    GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA
    GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA
    ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG
    GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA
    AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC
    CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT
    GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT
    TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT
    CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG
    TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC
    TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT
    AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT
    AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT
    GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC
    AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC
    ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA
    ATGAAAATGT TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC
    CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG
    CAATTAAACC TGGCCATGAT TAACTACGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC
    CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT
    GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT
    TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC
    TGTCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG
    CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG
    CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA
    ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA
    TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG
    GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC
    ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG
    GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA
    GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA
    AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT
    ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG
    TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT
    TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG
    AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA
    AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG
    ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG
    GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT
    CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG
    AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG
    CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA
    CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG
    AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC
    AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC
    TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG
    CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA
    TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG
    AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA
    GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT
    AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC
    ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA
    CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA
    CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA
    TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT
    GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC
    GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC
    TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT
    GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC
    ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG
    GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT
    GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG
    GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT
    AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC
    AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA
    ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT
    TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT
    TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA
    AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG
    AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA
    CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT
    GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT
    ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG
    AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA
    GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA
    ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG
    TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT
    CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT
    TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT
    GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG
    GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA
    GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA
    GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA
    AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC
    ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA
    TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG
    AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG
    CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG
    CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA
    TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC
    AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA
    GAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218658
    Clone ID Related Accession (Same CDS sequence) XM_010801678.3
    Accession Version XM_010801678.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5271bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGCAC GGAGCTTGCT GGTTTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799980.2
    CDS2836..8106
    Translation

    Target ORF information:

    RefSeq Version XM_010801678.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801678.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGCAC GGAGCTTGCT GGTTTTATTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218659
    Clone ID Related Accession (Same CDS sequence) XM_010801679.3
    Accession Version XM_010801679.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010801679.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
    TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT
    TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT
    CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG
    GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT
    GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG
    CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA
    CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT
    TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT
    GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG
    CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT
    GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA
    TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGTTGAG AAGTTTAATT
    CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA
    GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TAAACCTGGC CATGATTAAC
    TACGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG
    TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT
    GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA
    GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGTC GTCTTCCTGG AGTGTTAACT
    TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA
    GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG
    AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT
    ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG
    TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT
    ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT
    AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA
    CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG
    CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA
    AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG
    TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG
    ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA
    GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT
    CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT
    GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC
    TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT
    AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA
    AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG
    CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT
    GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT
    AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG
    TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA
    GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG
    CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT
    AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC
    TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA
    TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG
    TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT
    GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT
    TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT
    AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC
    ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA
    AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG
    TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT
    TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC
    ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC
    TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT
    CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC
    ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG
    ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC
    AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC
    GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT
    ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG
    CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG
    GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC
    TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC
    TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA
    CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG
    TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA
    CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA
    GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA
    GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG
    TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT
    TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA
    ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG
    CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT
    CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA
    AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT
    GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG
    GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT
    GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT
    GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA
    AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA
    AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC
    TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC
    TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG
    TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG
    CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799981.1
    CDS134..5398
    Translation

    Target ORF information:

    RefSeq Version XM_010801679.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801679.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
    TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT
    TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT
    CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG
    GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT
    GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG
    CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA
    CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT
    TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT
    GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG
    CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT
    GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA
    TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGTTGAG AAGTTTAATT
    CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA
    GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TAAACCTGGC CATGATTAAC
    TACGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG
    TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT
    GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA
    GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGTC GTCTTCCTGG AGTGTTAACT
    TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA
    GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG
    AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT
    ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG
    TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT
    ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT
    AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA
    CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG
    CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA
    AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG
    TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG
    ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA
    GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT
    CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT
    GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC
    TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT
    AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA
    AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG
    CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT
    GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT
    AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG
    TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA
    GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG
    CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT
    AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC
    TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA
    TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG
    TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT
    GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT
    TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT
    AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC
    ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA
    AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG
    TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT
    TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC
    ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC
    TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT
    CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC
    ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG
    ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC
    AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC
    GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT
    ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG
    CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG
    GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC
    TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC
    TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA
    CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG
    TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA
    CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA
    GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA
    GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG
    TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT
    TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA
    ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG
    CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT
    CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA
    AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT
    GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG
    GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT
    GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT
    GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA
    AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA
    AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC
    TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC
    TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG
    TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG
    CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218660
    Clone ID Related Accession (Same CDS sequence) XM_024999925.1
    Accession Version XM_024999925.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCTGAAA ATCACCTGCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
    TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG
    GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA
    GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT
    CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCAAAC ACCAGCTAGT
    AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA
    TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA
    ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT
    TGTGACTGTC ATCCTGAATT TGTACCGCCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA
    CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA
    GTAGATTTAA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC CGCACACCAG
    GATTTTGCTA ATATAATGAA AATGTTGAGA AGTTTAATTC AGGATGGCTA CACGGCCTTG
    CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA GCCTTCACAG AGTTGCTCAA CGGTTTAGAT
    CCTCAAAAAA TAAAGCAATT AAACCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA
    CTTGCCATTT CACTCCTTGG AATAGGACAG CCCGAGGAGT TATCTGAAGC TGAAAACCAG
    TTCAAGAGGA TGATTGAACA CTACCCCAAT GAGGGGCTTG ATTGCTTGGC CTACTGTGGA
    ATTGGAAAAG TGTATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA
    GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGGTT
    ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AGAAATTTGT TGAAGAATGC
    AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA
    ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGGATCAG CTGTTGCCAG
    TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT
    GATAAAATTG ACAAGGATTT TCTGCAAGGA ATCTGTCTTA CTCCTGATTG TGAAGGAATC
    ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG
    GTCATTAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG
    AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA
    AAATTAGCAC AAGAAAGACT GGAAGAGGAC TTAAGGGAAA GTAACCCACC AAAAACTGAA
    GAGCAGAGAG AAACTATCAA CCATGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG
    CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT
    CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT
    AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTCATTC GTCATTTGAT CCAAGAAAAG
    AATAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGACCCA
    AAACTAGCCA CCTGGATCCA GAAACTGAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC
    TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG
    AATGAGAAAT ACGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT
    GAGCCTACAT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTAGAAGA ACACAGAGAC
    AAGTTCCCAG CACTGCATAA TGCCTTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC
    ACTGTATTAA GGAAACAGGA CAGTGGTGAC GTGCCATTTA ATTCTACCAA GATAAAAAAC
    AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT
    TCAGTCACAC CAAACAATGA GACTGTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA
    AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATATAGA AGAATTTGAA
    GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA
    AACCCAAAAC AGAAATGTTC AACTCTATAC GATTATTTCT CTCAGTTGTT GGAGGAACAT
    GGTCCCTTGG ACATGAGTAA CAAAATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA
    ACTCAACAGA TACTAGAAAA AGCAGGAGGC TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT
    TTTGTTGTGA TTGATAACTG TATCGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG
    AGAAAGAAGA AAAACATTAA AACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA
    CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA
    CCAGTGTCTG ACTTACCTTC AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCCTGCACCT
    GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTT CGATAATTCT
    TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATGT CTTCTGATTC TCCCAGGCCA
    GTCTTTGAGG ATACCGGTCA CAAGCCGGTC TCCTCTAATT CTCCCAAACC AGTTCCTGAG
    GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT
    CCCTTTCGGG GATTTGACAT TACCCAGACT CCACCAGCCT TCATCAGTGT GTTACCGACT
    TTGCCCCAGT ATAGCATTTA CACCCCTCTG GCCAGTGTTC CTACTGAGTA TCACCTGCAA
    AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG
    GCTCATCATT TCAGTGCTGA GAATGCCCCT GGTACCCAGA TTGCCTCCGA AACACAGATC
    CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTATGATGG TGCTGATATA
    GTCCAGAGTG AGGCTAGCAG AAACGATGAC TACTGTGGCG ATTCTGCCAA ATGGGAAGTA
    AATCCAGAAA GCACCAATGA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG
    GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA
    CAACAGGGGG GTATTTCACA GGTTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG
    GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAAGGGCT CAGAAGAAAC CAAGGACTTG
    GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG
    TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA
    GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TAAATGCCAG CAAAATGTAT
    ACGCAGGAAA ATGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA
    ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA
    GAGCATTATG AAGAGAGTCT TCAAAGGGCT GTGGATGCAG AGGTATCTGT GCTTGAAAAC
    TGGAAGGACA TGGAAGTGTG TAAGTTACAG AGCATGGAGT CACAAGCCGA AAGGTTTCTC
    AAGAATCTGA AGCTAATGAG CAGTGATTCG GCAGCGTATT CTGACATGGA CTCAGACATA
    CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG
    TTTGAAGAAC AAATTCAGGC AATTAAATAT GGTTCTCAGC TCAGTGAACT TCCTAAAGTG
    CAGATTTCTG AGCTTTCATT TCCTACCTGT AACACGATTC ATCCTAAATT ACTTCCTGAG
    TCTTCAGGCC AGGATGGTCA AGGTCCCTCC ACTTCTACAA GTCATGGGAC CGGAGACCAG
    ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC
    TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA GAGCATGAGG GTGCTGGCCA GGCAACTGGG
    CCAGAACCAG GCCCAGTGGC TGATCAGAAG TTACCTGCTG CTCCAGAGCG TGCCACTCAT
    TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTT GGTGGTGTTC
    CCGTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG
    ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACAGA ACACGTCCTT
    GATGAAGAAA GAAAGAAAAA GCCAAGTCCA GGAAAGGGCT GGAGGCTGTC TGCACCCAGC
    TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG GACCCGCCCT CCATGCCTGT CGGAGCATCG
    CCCGAAACCA GGGGGCCGAG GACCGAGGAC GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA
    ATGTGCCGAG AAGTATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT
    CACAAGGGGT GCTTCAAGCA GTGGCTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT
    CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855693.1
    CDS90..5273
    Translation

    Target ORF information:

    RefSeq Version XM_024999925.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999925.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCCTGAAA ATCACCTGCT TTATGGTAAC CGAGCTCTTT GTTTTCTTCG TACTGGACAA 
    TTCAGAAATG CACTTGGTGA TGGAAAGAGA GCTACCATTC TGAAGAACAA TTGGACAAAG
    GGTCATTATC GTTATTGTGC TGCTCTTTCT ATGCTGGGGG AATATGACTG GGCCCTGCAA
    GCAAACATAA AAGCTCAAAA ACTCTGTAAA AATGACCCTG AGGGAATCAA GGATCTAATT
    CAGCAGCATG TAAAGTTACA AAAACAAATA GAAGACCTGC AAGGTCAAAC ACCAGCTAGT
    AATCCAGTCA AAGCCTTCTG TGAAAGCAGG GCCTACATAC CTAGTTTATC AGCACCTGCA
    TTTAGTACTT CACTTAACTT TGTGGAAACT GAAAAAAATT TCAGAAAAAC TAACCACGAA
    ATGGCAAACG GTGGTAATCA GAATCCAAAG GTGGTAGATG AAGCTCTGAA AGTAGATGAT
    TGTGACTGTC ATCCTGAATT TGTACCGCCA TCAAGTCAGC CTCCAAAATA TAAAGGGAAA
    CAAAAATCTC GAAACAATGA ATTGGAGAAG TTCAGCCCTG GTTCACAAGA AACTTTACCA
    GTAGATTTAA AAAACATCTT GGAGAAACAG TTTTCTAAAT CTTCCAGAGC CGCACACCAG
    GATTTTGCTA ATATAATGAA AATGTTGAGA AGTTTAATTC AGGATGGCTA CACGGCCTTG
    CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA GCCTTCACAG AGTTGCTCAA CGGTTTAGAT
    CCTCAAAAAA TAAAGCAATT AAACCTGGCC ATGATTAACT ACGTGTTAGT AGTCTACGGA
    CTTGCCATTT CACTCCTTGG AATAGGACAG CCCGAGGAGT TATCTGAAGC TGAAAACCAG
    TTCAAGAGGA TGATTGAACA CTACCCCAAT GAGGGGCTTG ATTGCTTGGC CTACTGTGGA
    ATTGGAAAAG TGTATTTGAA AAAAAACAGA TTTTTAGAAG CTCTTAATCA TTTTGAGAAA
    GCAAGAACCT TGATCTGTCG TCTTCCTGGA GTGTTAACTT GGCCCACAAG TAATGTGGTT
    ATTGAGGAGA CTCAGCCAGG GAAGATAAAG ATGCTGTTAG AGAAATTTGT TGAAGAATGC
    AGGTTCCCTC CAGTGCCGGA TGCCACTTGT TGCTATCAGA AGTGCCATGG ATATTCCAAA
    ATCCAGATAT ATATAACTGA TCCAGACTTT AAGGGTTTTA TACGGATCAG CTGTTGCCAG
    TATTGTAAAA TAGAATTTCA CATGAACTGC TGGAAGAAGT TAAAAACAAC AACCTTCAAT
    GATAAAATTG ACAAGGATTT TCTGCAAGGA ATCTGTCTTA CTCCTGATTG TGAAGGAATC
    ATTTCAAAGA TTATCATCTT CAGCAGTGGT GGTCAAGTTA AATGTGAATT TGAACACAAG
    GTCATTAAAG AAAAGGTTCC TCCAAGACCT ATTCTGAAAC AGAAATGTTC TAGCCTAGAG
    AAGTTGAGGC TGAAAGAAGA CAAAAAATTG AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA
    AAATTAGCAC AAGAAAGACT GGAAGAGGAC TTAAGGGAAA GTAACCCACC AAAAACTGAA
    GAGCAGAGAG AAACTATCAA CCATGTCCAA AGTTGCCAGT TCCTTGATGA CAGAATTCTG
    CAGTGCATAA AGCAGTACGC TGACAAGATT AAGTCCGGGA TACTGAATAC TTCCAAGCTT
    CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA ACCACAGAAG ACTACACAAC GTGTTTTTCT
    AGCAGAAATT TCCTGAATGA AGCAGTGGAC TATGTCATTC GTCATTTGAT CCAAGAAAAG
    AATAGAGTAA AGACAAGGGT ATTTCTGCAT GTTTTGAGTG AGCTTAAAGA AGTGGACCCA
    AAACTAGCCA CCTGGATCCA GAAACTGAAT AGCTTTGGCT TAGATGCCAC AGGACCTTTC
    TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG CTTGATTTTA GCATCATGAC TTTTCTCTGG
    AATGAGAAAT ACGGTCATAA GCTAGGCTCT ATAGAAGGAA AGCAACTTGA TTATTTCTAT
    GAGCCTACAT CACTGAAGGA AGCGCGGTGT TTAATATGGC TGCTAGAAGA ACACAGAGAC
    AAGTTCCCAG CACTGCATAA TGCCTTGGAC GAGTTCTTTG ATATAATGGA CAGCCGCTGC
    ACTGTATTAA GGAAACAGGA CAGTGGTGAC GTGCCATTTA ATTCTACCAA GATAAAAAAC
    AAAGGCAAGA AAAAGAAGCC AAAAGATTCA AAGCCTATGT TAGTTGGATC TGGAACACCT
    TCAGTCACAC CAAACAATGA GACTGTCACT TCAGGTGAAG ACCATATCAA ACGCAGTTTA
    AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT CACCTTCGGC AGGATATAGA AGAATTTGAA
    GCTCTGTATG AACAGCATAG TAATGAGTAT GTTGTCCGTA ATAAGAAGCT GTGGGACATA
    AACCCAAAAC AGAAATGTTC AACTCTATAC GATTATTTCT CTCAGTTGTT GGAGGAACAT
    GGTCCCTTGG ACATGAGTAA CAAAATGTTT TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA
    ACTCAACAGA TACTAGAAAA AGCAGGAGGC TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT
    TTTGTTGTGA TTGATAACTG TATCGCATTG AAGAAAGTTG CATTACGGCT CAAGAAAAAG
    AGAAAGAAGA AAAACATTAA AACAAAAGTA GAAGAAATTT CAAAAACAGG AGAGTATTTA
    CGAGTTAAAC TACCACTGAA TCCAACTGCT AGGGAATTTA AACCAGATGT AAAGTCCAAA
    CCAGTGTCTG ACTTACCTTC AGCACCAGCT TCTGAGGACA TGAAGCCTCA GCCTGCACCT
    GCTAATTCTC CCCAATCAGC TCGTGACACT GGGAAGCCAA AACCAGTTTT CGATAATTCT
    TCCAGATCAG TTTCTGAGGA TGAGAAGCCC AAAGGGATGT CTTCTGATTC TCCCAGGCCA
    GTCTTTGAGG ATACCGGTCA CAAGCCGGTC TCCTCTAATT CTCCCAAACC AGTTCCTGAG
    GAAGTGAAAG CGACCTATTG GCCTCGACCC CAGTTGGTCA CAGGATACTG TACATATCTT
    CCCTTTCGGG GATTTGACAT TACCCAGACT CCACCAGCCT TCATCAGTGT GTTACCGACT
    TTGCCCCAGT ATAGCATTTA CACCCCTCTG GCCAGTGTTC CTACTGAGTA TCACCTGCAA
    AGACCAATAC CAGTGGTGCC GTCTTTTGTA GCCAGTGACA TAACGGAAGT GCATTTTGAG
    GCTCATCATT TCAGTGCTGA GAATGCCCCT GGTACCCAGA TTGCCTCCGA AACACAGATC
    CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG TCAGAGTGCA GCTATGATGG TGCTGATATA
    GTCCAGAGTG AGGCTAGCAG AAACGATGAC TACTGTGGCG ATTCTGCCAA ATGGGAAGTA
    AATCCAGAAA GCACCAATGA AGTAACAAAT ATTCCATGTA CACAAGTGGT TGCTGTACAG
    GTGTCTTGGA ATATAACACA TCAGGAAGTC AATACTGAGC CATATGGTCC TTTTGAGGCA
    CAACAGGGGG GTATTTCACA GGTTGAAAAG GAGTACCAGG TTTTACAAGA GCAGCTTAAG
    GAAGCATGTG AAAATTATGA ACACAGCAAT GTCAAGGGCT CAGAAGAAAC CAAGGACTTG
    GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA AATAAGATCT CAAAGACAGA ACTAGATTGG
    TTCCTTGAAG ATTTGAACAA GGAAATTAAA AAATGGGAAC AGGAGAAAAA AGAAATCCAA
    GAAAGACTAA AGGCACTGAA GAAGAAAATG AAAAAGGTGT TAAATGCCAG CAAAATGTAT
    ACGCAGGAAA ATGATGGAAA GGAAAAGGCA CTTGAATTAC TTCTGGATCA GTCCCTTGAA
    ATCAGTAATA CATTTACAAA TGAGAAAATG AAAATAGAAG AGAGTATAAA GAAAGGGAAA
    GAGCATTATG AAGAGAGTCT TCAAAGGGCT GTGGATGCAG AGGTATCTGT GCTTGAAAAC
    TGGAAGGACA TGGAAGTGTG TAAGTTACAG AGCATGGAGT CACAAGCCGA AAGGTTTCTC
    AAGAATCTGA AGCTAATGAG CAGTGATTCG GCAGCGTATT CTGACATGGA CTCAGACATA
    CATTCATGGG AATCGTTTCT TTCTGATGTT AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG
    TTTGAAGAAC AAATTCAGGC AATTAAATAT GGTTCTCAGC TCAGTGAACT TCCTAAAGTG
    CAGATTTCTG AGCTTTCATT TCCTACCTGT AACACGATTC ATCCTAAATT ACTTCCTGAG
    TCTTCAGGCC AGGATGGTCA AGGTCCCTCC ACTTCTACAA GTCATGGGAC CGGAGACCAG
    ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC
    TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA GAGCATGAGG GTGCTGGCCA GGCAACTGGG
    CCAGAACCAG GCCCAGTGGC TGATCAGAAG TTACCTGCTG CTCCAGAGCG TGCCACTCAT
    TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT AGCATTATTG AGCACCTGTT GGTGGTGTTC
    CCGTGTTACA GCAGCACGGA GCTTGCTGGT TTTATTAAAA AAGTTAGAAA CAAAAACAAG
    ACCTCTCTGT CTGGATTGAG TATTGACGAA ATTGTGCAAA GAGTGACAGA ACACGTCCTT
    GATGAAGAAA GAAAGAAAAA GCCAAGTCCA GGAAAGGGCT GGAGGCTGTC TGCACCCAGC
    TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG GACCCGCCCT CCATGCCTGT CGGAGCATCG
    CCCGAAACCA GGGGGCCGAG GACCGAGGAC GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA
    ATGTGCCGAG AAGTATTCAA GTCCAAACAC GTGCGTGAGC TGCAGTGTGG GCACAGGCTT
    CACAAGGGGT GCTTCAAGCA GTGGCTGAAG AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT
    CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218661
    Clone ID Related Accession (Same CDS sequence) XM_005202060.2 , XM_005202061.4 , XM_024999992.1
    Accession Version XM_005202060.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202060.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202117.1
    CDS265..5298
    Translation

    Target ORF information:

    RefSeq Version XM_005202060.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202060.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218661
    Clone ID Related Accession (Same CDS sequence) XM_005202060.2 , XM_005202061.4 , XM_024999992.1
    Accession Version XM_005202061.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005202061.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202118.1
    CDS416..5449
    Translation

    Target ORF information:

    RefSeq Version XM_005202061.4
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202061.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218661
    Clone ID Related Accession (Same CDS sequence) XM_005202060.2 , XM_005202061.4 , XM_024999992.1
    Accession Version XM_024999992.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855760.1
    CDS179..5212
    Translation

    Target ORF information:

    RefSeq Version XM_024999992.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999992.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGTTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT AAACCTGGCC
    ATGATTAACT ACGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGTCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16127
    Clone ID Related Accession (Same CDS sequence) XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
    Accession Version NM_001109767.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DAAA02003579.1 and DAAA02003580.1. On Oct 1, 2015 this sequence version replaced NM_001109767.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments and orthologs. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001103237.2
    CDS1..5958
    Translation

    Target ORF information:

    RefSeq Version NM_001109767.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001109767.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16127
    Clone ID Related Accession (Same CDS sequence) XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
    Accession Version XM_005202058.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202115.1
    CDS78..6035
    Translation

    Target ORF information:

    RefSeq Version XM_005202058.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202058.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16127
    Clone ID Related Accession (Same CDS sequence) XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
    Accession Version XM_005202057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202114.1
    CDS3313..9270
    Translation

    Target ORF information:

    RefSeq Version XM_005202057.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16127
    Clone ID Related Accession (Same CDS sequence) XM_005202058.3 , XM_005202057.3 , NM_001109767.3 , XM_005202056.3
    Accession Version XM_005202056.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5958bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202113.1
    CDS115..6072
    Translation

    Target ORF information:

    RefSeq Version XM_005202056.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202056.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT GTGTCCTGCG CAAATTCCTG TGAAATGTGC
    CGAGAAGTAT TCAAGTCCAA ACACGTGCGT GAGCTGCAGT GTGGGCACAG GCTTCACAAG
    GGGTGCTTCA AGCAGTGGCT GAAGAGCCAG CGCACCTGCC CAGCCTGCCT GGGTCACGAC
    CTCCTGTCAG AGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16129
    Clone ID Related Accession (Same CDS sequence) XM_005202061.3 , XM_005202060.1
    Accession Version XM_005202061.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202061.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC
    ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202118.1
    CDS330..5363
    Translation

    Target ORF information:

    RefSeq Version XM_005202061.3
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202061.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC
    ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16129
    Clone ID Related Accession (Same CDS sequence) XM_005202061.3 , XM_005202060.1
    Accession Version XM_005202060.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5034bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC
    ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005202117.1
    CDS198..5231
    Translation

    Target ORF information:

    RefSeq Version XM_005202060.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005202060.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGCTGGGGG AATATGACTG GGCCCTGCAA GCAAACATAA AAGCTCAAAA ACTCTGTAAA 
    AATGACCCTG AGGGAATCAA GGATCTAATT CAGCAGCATG TAAAGTTACA AAAACAAATA
    GAAGACCTGC AAGGTCAAAC ACCAGCTAGT AATCCAGTCA AAGCCTTCTG TGAAAGCAGG
    GCCTACATAC CTAGTTTATC AGCACCTGCA TTTAGTACTT CACTTAACTT TGTGGAAACT
    GAAAAAAATT TCAGAAAAAC TAACCACGAA ATGGCAAACG GTGGTAATCA GAATCCAAAG
    GTGGTAGATG AAGCTCTGAA AGTAGATGAT TGTGACTGTC ATCCTGAATT TGTACCGCCA
    TCAAGTCAGC CTCCAAAATA TAAAGGGAAA CAAAAATCTC GAAACAATGA ATTGGAGAAG
    TTCAGCCCTG GTTCACAAGA AACTTTACCA GTAGATTTAA AAAACATCTT GGAGAAACAG
    TTTTCTAAAT CTTCCAGAGC CGCACACCAG GATTTTGCTA ATATAATGAA AATGCTGAGA
    AGTTTAATTC AGGATGGCTA CACGGCCTTG CTGGAGCAGC GCTGCCGCAG CGCCGCCCAA
    GCCTTCACAG AGTTGCTCAA CGGTTTAGAT CCTCAAAAAA TAAAGCAATT GAACCTGGCC
    ATGATTAACT ATGTGTTAGT AGTCTACGGA CTTGCCATTT CACTCCTTGG AATAGGACAG
    CCCGAGGAGT TATCTGAAGC TGAAAACCAG TTCAAGAGGA TGATTGAACA CTACCCCAAT
    GAGGGGCTTG ATTGCTTGGC CTACTGTGGA ATTGGAAAAG TGTATTTGAA AAAAAACAGA
    TTTTTAGAAG CTCTTAATCA TTTTGAGAAA GCAAGAACCT TGATCTGCCG TCTTCCTGGA
    GTGTTAACTT GGCCCACAAG TAATGTGGTT ATTGAGGAGA CTCAGCCAGG GAAGATAAAG
    ATGCTGTTAG AGAAATTTGT TGAAGAATGC AGGTTCCCTC CAGTGCCGGA TGCCACTTGT
    TGCTATCAGA AGTGCCATGG ATATTCCAAA ATCCAGATAT ATATAACTGA TCCAGACTTT
    AAGGGTTTTA TACGGATCAG CTGTTGCCAG TATTGTAAAA TAGAATTTCA CATGAACTGC
    TGGAAGAAGT TAAAAACAAC AACCTTCAAT GATAAAATTG ACAAGGATTT TCTGCAAGGA
    ATCTGTCTTA CTCCTGATTG TGAAGGAATC ATTTCAAAGA TTATCATCTT CAGCAGTGGT
    GGTCAAGTTA AATGTGAATT TGAACACAAG GTCATTAAAG AAAAGGTTCC TCCAAGACCT
    ATTCTGAAAC AGAAATGTTC TAGCCTAGAG AAGTTGAGGC TGAAAGAAGA CAAAAAATTG
    AAGCGAAAGC TCCAAAAAAA GGAAGCAAAA AAATTAGCAC AAGAAAGACT GGAAGAGGAC
    TTAAGGGAAA GTAACCCACC AAAAACTGAA GAGCAGAGAG AAACTATCAA CCATGTCCAA
    AGTTGCCAGT TCCTTGATGA CAGAATTCTG CAGTGCATAA AGCAGTACGC TGACAAGATT
    AAGTCCGGGA TACTGAATAC TTCCAAGCTT CTCAAAGAAT TGCTTTCTTG GAAGGTTTTA
    ACCACAGAAG ACTACACAAC GTGTTTTTCT AGCAGAAATT TCCTGAATGA AGCAGTGGAC
    TATGTCATTC GTCATTTGAT CCAAGAAAAG AATAGAGTAA AGACAAGGGT ATTTCTGCAT
    GTTTTGAGTG AGCTTAAAGA AGTGGACCCA AAACTAGCCA CCTGGATCCA GAAACTGAAT
    AGCTTTGGCT TAGATGCCAC AGGACCTTTC TTTTCTCGTT ATGGGGCTCC TCTTAAGGAG
    CTTGATTTTA GCATCATGAC TTTTCTCTGG AATGAGAAAT ACGGTCATAA GCTAGGCTCT
    ATAGAAGGAA AGCAACTTGA TTATTTCTAT GAGCCTACAT CACTGAAGGA AGCGCGGTGT
    TTAATATGGC TGCTAGAAGA ACACAGAGAC AAGTTCCCAG CACTGCATAA TGCCTTGGAC
    GAGTTCTTTG ATATAATGGA CAGCCGCTGC ACTGTATTAA GGAAACAGGA CAGTGGTGAC
    GTGCCATTTA ATTCTACCAA GATAAAAAAC AAAGGCAAGA AAAAGAAGCC AAAAGATTCA
    AAGCCTATGT TAGTTGGATC TGGAACACCT TCAGTCACAC CAAACAATGA GACTGTCACT
    TCAGGTGAAG ACCATATCAA ACGCAGTTTA AGTTCTGCGG GCCCCTTTGT AGTGCCTGAT
    CACCTTCGGC AGGATATAGA AGAATTTGAA GCTCTGTATG AACAGCATAG TAATGAGTAT
    GTTGTCCGTA ATAAGAAGCT GTGGGACATA AACCCAAAAC AGAAATGTTC AACTCTATAC
    GATTATTTCT CTCAGTTGTT GGAGGAACAT GGTCCCTTGG ACATGAGTAA CAAAATGTTT
    TCTGAAGAAT ATGAGTTCTT CCCAGAAGAA ACTCAACAGA TACTAGAAAA AGCAGGAGGC
    TTGAAAGCGT TTCTCCTGGG ATGTCCTCGT TTTGTTGTGA TTGATAACTG TATCGCATTG
    AAGAAAGTTG CATTACGGCT CAAGAAAAAG AGAAAGAAGA AAAACATTAA AACAAAAGTA
    GAAGAAATTT CAAAAACAGG AGAGTATTTA CGAGTTAAAC TACCACTGAA TCCAACTGCT
    AGGGAATTTA AACCAGATGT AAAGTCCAAA CCAGTGTCTG ACTTACCTTC AGCACCAGCT
    TCTGAGGACA TGAAGCCTCA GCCTGCACCT GCTAATTCTC CCCAATCAGC TCGTGACACT
    GGGAAGCCAA AACCAGTTTT CGATAATTCT TCCAGATCAG TTTCTGAGGA TGAGAAGCCC
    AAAGGGATGT CTTCTGATTC TCCCAGGCCA GTCTTTGAGG ATACCGGTCA CAAGCCGGTC
    TCCTCTAATT CTCCCAAACC AGTTCCTGAG GAAGTGAAAG CGACCTATTG GCCTCGACCC
    CAGTTGGTCA CAGGATACTG TACATATCTT CCCTTTCGGG GATTTGACAT TACCCAGACT
    CCACCAGCCT TCATCAGTGT GTTACCGACT TTGCCCCAGT ATAGCATTTA CACCCCTCTG
    GCCAGTGTTC CTACTGAGTA TCACCTGCAA AGACCAATAC CAGTGGTGCC GTCTTTTGTA
    GCCAGTGACA TAACGGAAGT GCATTTTGAG GCTCATCATT TCAGTGCTGA GAATGCCCCT
    GGTACCCAGA TTGCCTCCGA AACACAGATC CTTGAGGAAT CTTTGGAAAC ACCAGTGAAG
    TCAGAGTGCA GCTATGATGG TGCTGATATA GTCCAGAGTG AGGCTAGCAG AAACGATGAC
    TACTGTGGCG ATTCTGCCAA ATGGGAAGTA AATCCAGAAA GCACCAATGA AGTAACAAAT
    ATTCCATGTA CACAAGTGGT TGCTGTACAG GTGTCTTGGA ATATAACACA TCAGGAAGTC
    AATACTGAGC CATATGGTCC TTTTGAGGCA CAACAGGGGG GTATTTCACA GGTTGAAAAG
    GAGTACCAGG TTTTACAAGA GCAGCTTAAG GAAGCATGTG AAAATTATGA ACACAGCAAT
    GTCAAGGGCT CAGAAGAAAC CAAGGACTTG GAAGAAAAGT TGAAAAGGAA TGTTGAAGAA
    AATAAGATCT CAAAGACAGA ACTAGATTGG TTCCTTGAAG ATTTGAACAA GGAAATTAAA
    AAATGGGAAC AGGAGAAAAA AGAAATCCAA GAAAGACTAA AGGCACTGAA GAAGAAAATG
    AAAAAGGTGT TAAATGCCAG CAAAATGTAT ACGCAGGAAA ATGATGGAAA GGAAAAGGCA
    CTTGAATTAC TTCTGGATCA GTCCCTTGAA ATCAGTAATA CATTTACAAA TGAGAAAATG
    AAAATAGAAG AGAGTATAAA GAAAGGGAAA GAGCATTATG AAGAGAGTCT TCAAAGGGCT
    GTGGATGCAG AGGTATCTGT GCTTGAAAAC TGGAAGGACA TGGAAGTGTG TAAGTTACAG
    AGCATGGAGT CACAAGCCGA AAGGTTTCTC AAGAATCTGA AGCTAATGAG CAGTGATTCG
    GCAGCGTATT CTGACATGGA CTCAGACATA CATTCATGGG AATCGTTTCT TTCTGATGTT
    AGAGAAGAAA TTGAGAAAGC AAAGTCTCAG TTTGAAGAAC AAATTCAGGC AATTAAATAT
    GGTTCTCAGC TCAGTGAACT TCCTAAAGTG CAGATTTCTG AGCTTTCATT TCCTACCTGT
    AACACGATTC ATCCTAAATT ACTTCCTGAG TCTTCAGGCC AGGATGGTCA AGGTCCCTCC
    ACTTCTACAA GTCATGGGAC CGGAGACCAG ACAGCAGCTC CCGAGGAGTC AAGTCTGGGC
    TCTGCAGCTG ACAGCCCAGT GGAGTTGCCC TCCTGCAGTC AGAAAGCCCC CCAGCTGCCA
    GAGCATGAGG GTGCTGGCCA GGCAACTGGG CCAGAACCAG GCCCAGTGGC TGATCAGAAG
    TTACCTGCTG CTCCAGAGCG TGCCACTCAT TCAAGCCAGT CTCCAAAGAA GCCGTTCAAT
    AGCATTATTG AGCACCTGTT GGTGGTGTTC CCGTGTTACA GCAGCACGGA GCTTGCTGGT
    TTTATTAAAA AAGTTAGAAA CAAAAACAAG ACCTCTCTGT CTGGATTGAG TATTGACGAA
    ATTGTGCAAA GAGTGACAGA ACACGTCCTT GATGAAGAAA GAAAGAAAAA GCCAAGTCCA
    GGAAAGGGCT GGAGGCTGTC TGCACCCAGC TCTGCTGTTG CTGTGCCCAG GGCTGCCCAG
    GACCCGCCCT CCATGCCTGT CGGAGCATCG CCCGAAACCA GGGGGCCGAG GACCGAGGAC
    GTCCCTGTGT CCTGCGCAAA TTCCTGTGAA ATGTGCCGAG AAGTATTCAA GTCCAAACAC
    GTGCGTGAGC TGCAGTGTGG GCACAGGCTT CACAAGGGGT GCTTCAAGCA GTGGCTGAAG
    AGCCAGCGCA CCTGCCCAGC CTGCCTGGGT CACGACCTCC TGTCAGAGGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46601
    Clone ID Related Accession (Same CDS sequence) XM_010801674.1
    Accession Version XM_010801674.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799976.1
    CDS12..5876
    Translation

    Target ORF information:

    RefSeq Version XM_010801674.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801674.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGCCAAG TCCAGGAAAG GGCTGGAGGC TGTCTGCACC CAGCTCTGCT
    GTTGCTGTGC CCAGGGCTGC CCAGGACCCG CCCTCCATGC CTGTCGGAGC ATCGCCCGAA
    ACCAGGGGGC CGAGGACCGA GGACGTCCCT TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46602
    Clone ID Related Accession (Same CDS sequence) XM_010801675.1
    Accession Version XM_010801675.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5823bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGGTGTC CTGCGCAAAT TCCTGTGAAA TGTGCCGAGA AGTATTCAAG
    TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAGGGGTG CTTCAAGCAG
    TGGCTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799977.1
    CDS12..5834
    Translation

    Target ORF information:

    RefSeq Version XM_010801675.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801675.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG ATTCATCCTA AATTACTTCC TGAGTCTTCA
    GGCCAGGATG GTCAAGGTCC CTCCACTTCT ACAAGTCATG GGACCGGAGA CCAGACAGCA
    GCTCCCGAGG AGTCAAGTCT GGGCTCTGCA GCTGACAGCC CAGTGGAGTT GCCCTCCTGC
    AGTCAGAAAG CCCCCCAGCT GCCAGAGCAT GAGGGTGCTG GCCAGGCAAC TGGGCCAGAA
    CCAGGCCCAG TGGCTGATCA GAAGTTACCT GCTGCTCCAG AGCGTGCCAC TCATTCAAGC
    CAGTCTCCAA AGAAGCCGTT CAATAGCATT ATTGAGCACC TGTTGGTGGT GTTCCCGTGT
    TACAGCAGCA CGGAGCTTGC TGGTTTTATT AAAAAAGTTA GAAACAAAAA CAAGACCTCT
    CTGTCTGGAT TGAGTATTGA CGAAATTGTG CAAAGAGTGA CAGAACACGT CCTTGATGAA
    GAAAGAAAGA AAAAGGTGTC CTGCGCAAAT TCCTGTGAAA TGTGCCGAGA AGTATTCAAG
    TCCAAACACG TGCGTGAGCT GCAGTGTGGG CACAGGCTTC ACAAGGGGTG CTTCAAGCAG
    TGGCTGAAGA GCCAGCGCAC CTGCCCAGCC TGCCTGGGTC ACGACCTCCT GTCAGAGGAG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46604
    Clone ID Related Accession (Same CDS sequence) XM_010801677.2
    Accession Version XM_010801677.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5649bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
    CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA
    TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG
    GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA
    GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA
    ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG
    GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA
    AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC
    CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT
    GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT
    TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT
    CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG
    TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC
    TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT
    AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT
    AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT
    GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC
    AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC
    ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA
    ATGAAAATGC TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC
    CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG
    CAATTGAACC TGGCCATGAT TAACTATGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC
    CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT
    GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT
    TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC
    TGCCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG
    CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG
    CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA
    ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA
    TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG
    GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC
    ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG
    GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA
    GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA
    AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT
    ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG
    TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT
    TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG
    AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA
    AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG
    ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG
    GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT
    CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG
    AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG
    CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA
    CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG
    AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC
    AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC
    TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG
    CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA
    TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG
    AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA
    GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT
    AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC
    ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA
    CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA
    CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA
    TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT
    GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC
    GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC
    TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT
    GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC
    ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG
    GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT
    GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG
    GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT
    AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC
    AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA
    ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT
    TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT
    TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA
    AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG
    AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA
    CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT
    GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT
    ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG
    AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA
    GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA
    ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG
    TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT
    CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT
    TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT
    GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG
    GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA
    GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA
    GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA
    AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC
    ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA
    TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG
    AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG
    CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG
    CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA
    TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC
    AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA
    GAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799979.1
    CDS232..5880
    Translation

    Target ORF information:

    RefSeq Version XM_010801677.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801677.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    ATGCACTCCT GTGTAAGGAC CAGCGGTTCT CATGCTTGTG AGATAAGTTT GAAGAAATTA 
    CAGCAGCTTG AACTGATGGA AGATATTGTG GATTTGGCAA AGAAAGTTGT GAATGATACA
    TTCTTTATCG GAGGCTTATT GAGAATTGGC TATAAAATAG AGAATAAAAT CTTGGCAATG
    GAAGAAGCTT TGAATTGGGT GAAATACACA GGCGATGTAA CAATTTTATC TAAATTAGGA
    GCAGTTGATA ATTGTTGGCG TATGTTAAGT ATTTTCTTTA CTGAATACAA GTATCACATA
    ACTAAAGTTG TAACAGAAAA CTGCAGTTTG CTTGAAGAAT TTAAAACTCA AAGTTGTGCG
    GAGTGTCTAG AGCAAGGAGA ACTAATGAAA ATGAAAGGAA ATGAAGAGTT TTCCAAAGAA
    AGATATGATA TAGCTATTAT CTATTACACC AGAGCCATTG AATGTAGGCC TGAAAATCAC
    CTGCTTTATG GTAACCGAGC TCTTTGTTTT CTTCGTACTG GACAATTCAG AAATGCACTT
    GGTGATGGAA AGAGAGCTAC CATTCTGAAG AACAATTGGA CAAAGGGTCA TTATCGTTAT
    TGTGCTGCTC TTTCTATGCT GGGGGAATAT GACTGGGCCC TGCAAGCAAA CATAAAAGCT
    CAAAAACTCT GTAAAAATGA CCCTGAGGGA ATCAAGGATC TAATTCAGCA GCATGTAAAG
    TTACAAAAAC AAATAGAAGA CCTGCAAGGT CAAACACCAG CTAGTAATCC AGTCAAAGCC
    TTCTGTGAAA GCAGGGCCTA CATACCTAGT TTATCAGCAC CTGCATTTAG TACTTCACTT
    AACTTTGTGG AAACTGAAAA AAATTTCAGA AAAACTAACC ACGAAATGGC AAACGGTGGT
    AATCAGAATC CAAAGGTGGT AGATGAAGCT CTGAAAGTAG ATGATTGTGA CTGTCATCCT
    GAATTTGTAC CGCCATCAAG TCAGCCTCCA AAATATAAAG GGAAACAAAA ATCTCGAAAC
    AATGAATTGG AGAAGTTCAG CCCTGGTTCA CAAGAAACTT TACCAGTAGA TTTAAAAAAC
    ATCTTGGAGA AACAGTTTTC TAAATCTTCC AGAGCCGCAC ACCAGGATTT TGCTAATATA
    ATGAAAATGC TGAGAAGTTT AATTCAGGAT GGCTACACGG CCTTGCTGGA GCAGCGCTGC
    CGCAGCGCCG CCCAAGCCTT CACAGAGTTG CTCAACGGTT TAGATCCTCA AAAAATAAAG
    CAATTGAACC TGGCCATGAT TAACTATGTG TTAGTAGTCT ACGGACTTGC CATTTCACTC
    CTTGGAATAG GACAGCCCGA GGAGTTATCT GAAGCTGAAA ACCAGTTCAA GAGGATGATT
    GAACACTACC CCAATGAGGG GCTTGATTGC TTGGCCTACT GTGGAATTGG AAAAGTGTAT
    TTGAAAAAAA ACAGATTTTT AGAAGCTCTT AATCATTTTG AGAAAGCAAG AACCTTGATC
    TGCCGTCTTC CTGGAGTGTT AACTTGGCCC ACAAGTAATG TGGTTATTGA GGAGACTCAG
    CCAGGGAAGA TAAAGATGCT GTTAGAGAAA TTTGTTGAAG AATGCAGGTT CCCTCCAGTG
    CCGGATGCCA CTTGTTGCTA TCAGAAGTGC CATGGATATT CCAAAATCCA GATATATATA
    ACTGATCCAG ACTTTAAGGG TTTTATACGG ATCAGCTGTT GCCAGTATTG TAAAATAGAA
    TTTCACATGA ACTGCTGGAA GAAGTTAAAA ACAACAACCT TCAATGATAA AATTGACAAG
    GATTTTCTGC AAGGAATCTG TCTTACTCCT GATTGTGAAG GAATCATTTC AAAGATTATC
    ATCTTCAGCA GTGGTGGTCA AGTTAAATGT GAATTTGAAC ACAAGGTCAT TAAAGAAAAG
    GTTCCTCCAA GACCTATTCT GAAACAGAAA TGTTCTAGCC TAGAGAAGTT GAGGCTGAAA
    GAAGACAAAA AATTGAAGCG AAAGCTCCAA AAAAAGGAAG CAAAAAAATT AGCACAAGAA
    AGACTGGAAG AGGACTTAAG GGAAAGTAAC CCACCAAAAA CTGAAGAGCA GAGAGAAACT
    ATCAACCATG TCCAAAGTTG CCAGTTCCTT GATGACAGAA TTCTGCAGTG CATAAAGCAG
    TACGCTGACA AGATTAAGTC CGGGATACTG AATACTTCCA AGCTTCTCAA AGAATTGCTT
    TCTTGGAAGG TTTTAACCAC AGAAGACTAC ACAACGTGTT TTTCTAGCAG AAATTTCCTG
    AATGAAGCAG TGGACTATGT CATTCGTCAT TTGATCCAAG AAAAGAATAG AGTAAAGACA
    AGGGTATTTC TGCATGTTTT GAGTGAGCTT AAAGAAGTGG ACCCAAAACT AGCCACCTGG
    ATCCAGAAAC TGAATAGCTT TGGCTTAGAT GCCACAGGAC CTTTCTTTTC TCGTTATGGG
    GCTCCTCTTA AGGAGCTTGA TTTTAGCATC ATGACTTTTC TCTGGAATGA GAAATACGGT
    CATAAGCTAG GCTCTATAGA AGGAAAGCAA CTTGATTATT TCTATGAGCC TACATCACTG
    AAGGAAGCGC GGTGTTTAAT ATGGCTGCTA GAAGAACACA GAGACAAGTT CCCAGCACTG
    CATAATGCCT TGGACGAGTT CTTTGATATA ATGGACAGCC GCTGCACTGT ATTAAGGAAA
    CAGGACAGTG GTGACGTGCC ATTTAATTCT ACCAAGATAA AAAACAAAGG CAAGAAAAAG
    AAGCCAAAAG ATTCAAAGCC TATGTTAGTT GGATCTGGAA CACCTTCAGT CACACCAAAC
    AATGAGACTG TCACTTCAGG TGAAGACCAT ATCAAACGCA GTTTAAGTTC TGCGGGCCCC
    TTTGTAGTGC CTGATCACCT TCGGCAGGAT ATAGAAGAAT TTGAAGCTCT GTATGAACAG
    CATAGTAATG AGTATGTTGT CCGTAATAAG AAGCTGTGGG ACATAAACCC AAAACAGAAA
    TGTTCAACTC TATACGATTA TTTCTCTCAG TTGTTGGAGG AACATGGTCC CTTGGACATG
    AGTAACAAAA TGTTTTCTGA AGAATATGAG TTCTTCCCAG AAGAAACTCA ACAGATACTA
    GAAAAAGCAG GAGGCTTGAA AGCGTTTCTC CTGGGATGTC CTCGTTTTGT TGTGATTGAT
    AACTGTATCG CATTGAAGAA AGTTGCATTA CGGCTCAAGA AAAAGAGAAA GAAGAAAAAC
    ATTAAAACAA AAGTAGAAGA AATTTCAAAA ACAGGAGAGT ATTTACGAGT TAAACTACCA
    CTGAATCCAA CTGCTAGGGA ATTTAAACCA GATGTAAAGT CCAAACCAGT GTCTGACTTA
    CCTTCAGCAC CAGCTTCTGA GGACATGAAG CCTCAGCCTG CACCTGCTAA TTCTCCCCAA
    TCAGCTCGTG ACACTGGGAA GCCAAAACCA GTTTTCGATA ATTCTTCCAG ATCAGTTTCT
    GAGGATGAGA AGCCCAAAGG GATGTCTTCT GATTCTCCCA GGCCAGTCTT TGAGGATACC
    GGTCACAAGC CGGTCTCCTC TAATTCTCCC AAACCAGTTC CTGAGGAAGT GAAAGCGACC
    TATTGGCCTC GACCCCAGTT GGTCACAGGA TACTGTACAT ATCTTCCCTT TCGGGGATTT
    GACATTACCC AGACTCCACC AGCCTTCATC AGTGTGTTAC CGACTTTGCC CCAGTATAGC
    ATTTACACCC CTCTGGCCAG TGTTCCTACT GAGTATCACC TGCAAAGACC AATACCAGTG
    GTGCCGTCTT TTGTAGCCAG TGACATAACG GAAGTGCATT TTGAGGCTCA TCATTTCAGT
    GCTGAGAATG CCCCTGGTAC CCAGATTGCC TCCGAAACAC AGATCCTTGA GGAATCTTTG
    GAAACACCAG TGAAGTCAGA GTGCAGCTAT GATGGTGCTG ATATAGTCCA GAGTGAGGCT
    AGCAGAAACG ATGACTACTG TGGCGATTCT GCCAAATGGG AAGTAAATCC AGAAAGCACC
    AATGAAGTAA CAAATATTCC ATGTACACAA GTGGTTGCTG TACAGGTGTC TTGGAATATA
    ACACATCAGG AAGTCAATAC TGAGCCATAT GGTCCTTTTG AGGCACAACA GGGGGGTATT
    TCACAGGTTG AAAAGGAGTA CCAGGTTTTA CAAGAGCAGC TTAAGGAAGC ATGTGAAAAT
    TATGAACACA GCAATGTCAA GGGCTCAGAA GAAACCAAGG ACTTGGAAGA AAAGTTGAAA
    AGGAATGTTG AAGAAAATAA GATCTCAAAG ACAGAACTAG ATTGGTTCCT TGAAGATTTG
    AACAAGGAAA TTAAAAAATG GGAACAGGAG AAAAAAGAAA TCCAAGAAAG ACTAAAGGCA
    CTGAAGAAGA AAATGAAAAA GGTGTTAAAT GCCAGCAAAA TGTATACGCA GGAAAATGAT
    GGAAAGGAAA AGGCACTTGA ATTACTTCTG GATCAGTCCC TTGAAATCAG TAATACATTT
    ACAAATGAGA AAATGAAAAT AGAAGAGAGT ATAAAGAAAG GGAAAGAGCA TTATGAAGAG
    AGTCTTCAAA GGGCTGTGGA TGCAGAGGTA TCTGTGCTTG AAAACTGGAA GGACATGGAA
    GTGTGTAAGT TACAGAGCAT GGAGTCACAA GCCGAAAGGT TTCTCAAGAA TCTGAAGCTA
    ATGAGCAGTG ATTCGGCAGC GTATTCTGAC ATGGACTCAG ACATACATTC ATGGGAATCG
    TTTCTTTCTG ATGTTAGAGA AGAAATTGAG AAAGCAAAGT CTCAGTTTGA AGAACAAATT
    CAGGCAATTA AATATGGTTC TCAGCTCAGT GAACTTCCTA AAGTGCAGAT TTCTGAGCTT
    TCATTTCCTA CCTGTAACAC GATTCATCCT AAATTACTTC CTGAGTCTTC AGGCCAGGAT
    GGTCAAGGTC CCTCCACTTC TACAAGTCAT GGGACCGGAG ACCAGACAGC AGCTCCCGAG
    GAGTCAAGTC TGGGCTCTGC AGCTGACAGC CCAGTGGAGT TGCCCTCCTG CAGTCAGAAA
    GCCCCCCAGC TGCCAGAGCA TGAGGGTGCT GGCCAGGCAA CTGGGCCAGA ACCAGGCCCA
    GTGGCTGATC AGAAGTTACC TGCTGCTCCA GAGCGTGCCA CTCATTCAAG CCAGTCTCCA
    AAGAAGCCGT TCAATAGCAT TATTGAGCAC CTGTTGGTGG TGTTCCCGTG TTACAGCAGC
    ACGGAGCTTG CTGGTTTTAT TAAAAAAGTT AGAAACAAAA ACAAGACCTC TCTGTCTGGA
    TTGAGTATTG ACGAAATTGT GCAAAGAGTG ACAGAACACG TCCTTGATGA AGAAAGAAAG
    AAAAAGCCAA GTCCAGGAAA GGGCTGGAGG CTGTCTGCAC CCAGCTCTGC TGTTGCTGTG
    CCCAGGGCTG CCCAGGACCC GCCCTCCATG CCTGTCGGAG CATCGCCCGA AACCAGGGGG
    CCGAGGACCG AGGACGTCCC TGTGTCCTGC GCAAATTCCT GTGAAATGTG CCGAGAAGTA
    TTCAAGTCCA AACACGTGCG TGAGCTGCAG TGTGGGCACA GGCTTCACAA GGGGTGCTTC
    AAGCAGTGGC TGAAGAGCCA GCGCACCTGC CCAGCCTGCC TGGGTCACGA CCTCCTGTCA
    GAGGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46605
    Clone ID Related Accession (Same CDS sequence) XM_010801678.2
    Accession Version XM_010801678.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5214bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG CACGGAGCTT GCTGGTTTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799980.1
    CDS12..5225
    Translation

    Target ORF information:

    RefSeq Version XM_010801678.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801678.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGCCGATT TTCCCCAGGG AAATTTTGCA GATTATGCCT TGTTAGAAGA TTGCCCTTAT 
    GTGGATGATT GTATCTTTGC CCCTGAGTTT ATGTCCAGTG ATTATGTTCG TGTGACTCAG
    CTTTACTGTG ATGGAGTGGG TAGACAGTAT AAAGATTATG CCCAAACTGA GGGAAACTTA
    GAATTTGACA TCTGCACTCT GTGGTGTAGT CAACCTATTT CTGTCCTGCA AGATTACTGT
    GATGCCATTA AAGTCCACAT CTTCTGGCCA CTTCTGTTTC AACGTCAGCA GAGCTCTATA
    ATAACACGGA TGCACTCCTG TGTAAGGACC AGCGGTTCTC ATGCTTGTGA GATAAGTTTG
    AAGAAATTAC AGCAGCTTGA ACTGATGGAA GATATTGTGG ATTTGGCAAA GAAAGTTGTG
    AATGATACAT TCTTTATCGG AGGCTTATTG AGAATTGGCT ATAAAATAGA GAATAAAATC
    TTGGCAATGG AAGAAGCTTT GAATTGGGTG AAATACACAG GCGATGTAAC AATTTTATCT
    AAATTAGGAG CAGTTGATAA TTGTTGGCGT ATGTTAAGTA TTTTCTTTAC TGAATACAAG
    TATCACATAA CTAAAGTTGT AACAGAAAAC TGCAGTTTGC TTGAAGAATT TAAAACTCAA
    AGTTGTGCGG AGTGTCTAGA GCAAGGAGAA CTAATGAAAA TGAAAGGAAA TGAAGAGTTT
    TCCAAAGAAA GATATGATAT AGCTATTATC TATTACACCA GAGCCATTGA ATGTAGGCCT
    GAAAATCACC TGCTTTATGG TAACCGAGCT CTTTGTTTTC TTCGTACTGG ACAATTCAGA
    AATGCACTTG GTGATGGAAA GAGAGCTACC ATTCTGAAGA ACAATTGGAC AAAGGGTCAT
    TATCGTTATT GTGCTGCTCT TTCTATGCTG GGGGAATATG ACTGGGCCCT GCAAGCAAAC
    ATAAAAGCTC AAAAACTCTG TAAAAATGAC CCTGAGGGAA TCAAGGATCT AATTCAGCAG
    CATGTAAAGT TACAAAAACA AATAGAAGAC CTGCAAGGTC AAACACCAGC TAGTAATCCA
    GTCAAAGCCT TCTGTGAAAG CAGGGCCTAC ATACCTAGTT TATCAGCACC TGCATTTAGT
    ACTTCACTTA ACTTTGTGGA AACTGAAAAA AATTTCAGAA AAACTAACCA CGAAATGGCA
    AACGGTGGTA ATCAGAATCC AAAGGTGGTA GATGAAGCTC TGAAAGTAGA TGATTGTGAC
    TGTCATCCTG AATTTGTACC GCCATCAAGT CAGCCTCCAA AATATAAAGG GAAACAAAAA
    TCTCGAAACA ATGAATTGGA GAAGTTCAGC CCTGGTTCAC AAGAAACTTT ACCAGTAGAT
    TTAAAAAACA TCTTGGAGAA ACAGTTTTCT AAATCTTCCA GAGCCGCACA CCAGGATTTT
    GCTAATATAA TGAAAATGCT GAGAAGTTTA ATTCAGGATG GCTACACGGC CTTGCTGGAG
    CAGCGCTGCC GCAGCGCCGC CCAAGCCTTC ACAGAGTTGC TCAACGGTTT AGATCCTCAA
    AAAATAAAGC AATTGAACCT GGCCATGATT AACTATGTGT TAGTAGTCTA CGGACTTGCC
    ATTTCACTCC TTGGAATAGG ACAGCCCGAG GAGTTATCTG AAGCTGAAAA CCAGTTCAAG
    AGGATGATTG AACACTACCC CAATGAGGGG CTTGATTGCT TGGCCTACTG TGGAATTGGA
    AAAGTGTATT TGAAAAAAAA CAGATTTTTA GAAGCTCTTA ATCATTTTGA GAAAGCAAGA
    ACCTTGATCT GCCGTCTTCC TGGAGTGTTA ACTTGGCCCA CAAGTAATGT GGTTATTGAG
    GAGACTCAGC CAGGGAAGAT AAAGATGCTG TTAGAGAAAT TTGTTGAAGA ATGCAGGTTC
    CCTCCAGTGC CGGATGCCAC TTGTTGCTAT CAGAAGTGCC ATGGATATTC CAAAATCCAG
    ATATATATAA CTGATCCAGA CTTTAAGGGT TTTATACGGA TCAGCTGTTG CCAGTATTGT
    AAAATAGAAT TTCACATGAA CTGCTGGAAG AAGTTAAAAA CAACAACCTT CAATGATAAA
    ATTGACAAGG ATTTTCTGCA AGGAATCTGT CTTACTCCTG ATTGTGAAGG AATCATTTCA
    AAGATTATCA TCTTCAGCAG TGGTGGTCAA GTTAAATGTG AATTTGAACA CAAGGTCATT
    AAAGAAAAGG TTCCTCCAAG ACCTATTCTG AAACAGAAAT GTTCTAGCCT AGAGAAGTTG
    AGGCTGAAAG AAGACAAAAA ATTGAAGCGA AAGCTCCAAA AAAAGGAAGC AAAAAAATTA
    GCACAAGAAA GACTGGAAGA GGACTTAAGG GAAAGTAACC CACCAAAAAC TGAAGAGCAG
    AGAGAAACTA TCAACCATGT CCAAAGTTGC CAGTTCCTTG ATGACAGAAT TCTGCAGTGC
    ATAAAGCAGT ACGCTGACAA GATTAAGTCC GGGATACTGA ATACTTCCAA GCTTCTCAAA
    GAATTGCTTT CTTGGAAGGT TTTAACCACA GAAGACTACA CAACGTGTTT TTCTAGCAGA
    AATTTCCTGA ATGAAGCAGT GGACTATGTC ATTCGTCATT TGATCCAAGA AAAGAATAGA
    GTAAAGACAA GGGTATTTCT GCATGTTTTG AGTGAGCTTA AAGAAGTGGA CCCAAAACTA
    GCCACCTGGA TCCAGAAACT GAATAGCTTT GGCTTAGATG CCACAGGACC TTTCTTTTCT
    CGTTATGGGG CTCCTCTTAA GGAGCTTGAT TTTAGCATCA TGACTTTTCT CTGGAATGAG
    AAATACGGTC ATAAGCTAGG CTCTATAGAA GGAAAGCAAC TTGATTATTT CTATGAGCCT
    ACATCACTGA AGGAAGCGCG GTGTTTAATA TGGCTGCTAG AAGAACACAG AGACAAGTTC
    CCAGCACTGC ATAATGCCTT GGACGAGTTC TTTGATATAA TGGACAGCCG CTGCACTGTA
    TTAAGGAAAC AGGACAGTGG TGACGTGCCA TTTAATTCTA CCAAGATAAA AAACAAAGGC
    AAGAAAAAGA AGCCAAAAGA TTCAAAGCCT ATGTTAGTTG GATCTGGAAC ACCTTCAGTC
    ACACCAAACA ATGAGACTGT CACTTCAGGT GAAGACCATA TCAAACGCAG TTTAAGTTCT
    GCGGGCCCCT TTGTAGTGCC TGATCACCTT CGGCAGGATA TAGAAGAATT TGAAGCTCTG
    TATGAACAGC ATAGTAATGA GTATGTTGTC CGTAATAAGA AGCTGTGGGA CATAAACCCA
    AAACAGAAAT GTTCAACTCT ATACGATTAT TTCTCTCAGT TGTTGGAGGA ACATGGTCCC
    TTGGACATGA GTAACAAAAT GTTTTCTGAA GAATATGAGT TCTTCCCAGA AGAAACTCAA
    CAGATACTAG AAAAAGCAGG AGGCTTGAAA GCGTTTCTCC TGGGATGTCC TCGTTTTGTT
    GTGATTGATA ACTGTATCGC ATTGAAGAAA GTTGCATTAC GGCTCAAGAA AAAGAGAAAG
    AAGAAAAACA TTAAAACAAA AGTAGAAGAA ATTTCAAAAA CAGGAGAGTA TTTACGAGTT
    AAACTACCAC TGAATCCAAC TGCTAGGGAA TTTAAACCAG ATGTAAAGTC CAAACCAGTG
    TCTGACTTAC CTTCAGCACC AGCTTCTGAG GACATGAAGC CTCAGCCTGC ACCTGCTAAT
    TCTCCCCAAT CAGCTCGTGA CACTGGGAAG CCAAAACCAG TTTTCGATAA TTCTTCCAGA
    TCAGTTTCTG AGGATGAGAA GCCCAAAGGG ATGTCTTCTG ATTCTCCCAG GCCAGTCTTT
    GAGGATACCG GTCACAAGCC GGTCTCCTCT AATTCTCCCA AACCAGTTCC TGAGGAAGTG
    AAAGCGACCT ATTGGCCTCG ACCCCAGTTG GTCACAGGAT ACTGTACATA TCTTCCCTTT
    CGGGGATTTG ACATTACCCA GACTCCACCA GCCTTCATCA GTGTGTTACC GACTTTGCCC
    CAGTATAGCA TTTACACCCC TCTGGCCAGT GTTCCTACTG AGTATCACCT GCAAAGACCA
    ATACCAGTGG TGCCGTCTTT TGTAGCCAGT GACATAACGG AAGTGCATTT TGAGGCTCAT
    CATTTCAGTG CTGAGAATGC CCCTGGTACC CAGATTGCCT CCGAAACACA GATCCTTGAG
    GAATCTTTGG AAACACCAGT GAAGTCAGAG TGCAGCTATG ATGGTGCTGA TATAGTCCAG
    AGTGAGGCTA GCAGAAACGA TGACTACTGT GGCGATTCTG CCAAATGGGA AGTAAATCCA
    GAAAGCACCA ATGAAGTAAC AAATATTCCA TGTACACAAG TGGTTGCTGT ACAGGTGTCT
    TGGAATATAA CACATCAGGA AGTCAATACT GAGCCATATG GTCCTTTTGA GGCACAACAG
    GGGGGTATTT CACAGGTTGA AAAGGAGTAC CAGGTTTTAC AAGAGCAGCT TAAGGAAGCA
    TGTGAAAATT ATGAACACAG CAATGTCAAG GGCTCAGAAG AAACCAAGGA CTTGGAAGAA
    AAGTTGAAAA GGAATGTTGA AGAAAATAAG ATCTCAAAGA CAGAACTAGA TTGGTTCCTT
    GAAGATTTGA ACAAGGAAAT TAAAAAATGG GAACAGGAGA AAAAAGAAAT CCAAGAAAGA
    CTAAAGGCAC TGAAGAAGAA AATGAAAAAG GTGTTAAATG CCAGCAAAAT GTATACGCAG
    GAAAATGATG GAAAGGAAAA GGCACTTGAA TTACTTCTGG ATCAGTCCCT TGAAATCAGT
    AATACATTTA CAAATGAGAA AATGAAAATA GAAGAGAGTA TAAAGAAAGG GAAAGAGCAT
    TATGAAGAGA GTCTTCAAAG GGCTGTGGAT GCAGAGGTAT CTGTGCTTGA AAACTGGAAG
    GACATGGAAG TGTGTAAGTT ACAGAGCATG GAGTCACAAG CCGAAAGGTT TCTCAAGAAT
    CTGAAGCTAA TGAGCAGTGA TTCGGCAGCG TATTCTGACA TGGACTCAGA CATACATTCA
    TGGGAATCGT TTCTTTCTGA TGTTAGAGAA GAAATTGAGA AAGCAAAGTC TCAGTTTGAA
    GAACAAATTC AGGCAATTAA ATATGGTTCT CAGCTCAGTG AACTTCCTAA AGTGCAGATT
    TCTGAGCTTT CATTTCCTAC CTGTAACACG CACGGAGCTT GCTGGTTTTA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46606
    Clone ID Related Accession (Same CDS sequence) XM_010801679.2
    Accession Version XM_010801679.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5265bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103826.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801679.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
    TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT
    TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT
    CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG
    GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT
    GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG
    CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA
    CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT
    TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT
    GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG
    CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT
    GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA
    TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGCTGAG AAGTTTAATT
    CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA
    GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TGAACCTGGC CATGATTAAC
    TATGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG
    TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT
    GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA
    GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGCC GTCTTCCTGG AGTGTTAACT
    TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA
    GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG
    AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT
    ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG
    TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT
    ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT
    AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA
    CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG
    CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA
    AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG
    TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG
    ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA
    GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT
    CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT
    GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC
    TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT
    AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA
    AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG
    CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT
    GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT
    AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG
    TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA
    GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG
    CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT
    AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC
    TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA
    TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG
    TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT
    GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT
    TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT
    AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC
    ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA
    AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG
    TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT
    TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC
    ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC
    TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT
    CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC
    ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG
    ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC
    AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC
    GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT
    ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG
    CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG
    GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC
    TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC
    TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA
    CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG
    TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA
    CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA
    GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA
    GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG
    TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT
    TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA
    ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG
    CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT
    CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA
    AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT
    GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG
    GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT
    GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT
    GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA
    AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA
    AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC
    TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC
    TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG
    TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG
    CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010799981.1
    CDS98..5362
    Translation

    Target ORF information:

    RefSeq Version XM_010801679.2
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010801679.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAAATGA AAGGAAATGA AGAGTTTTCC AAAGAAAGAT ATGATATAGC TATTATCTAT 
    TACACCAGAG CCATTGAATG TAGGCCTGAA AATCACCTGC TTTATGGTAA CCGAGCTCTT
    TGTTTTCTTC GTACTGGACA ATTCAGAAAT GCACTTGGTG ATGGAAAGAG AGCTACCATT
    CTGAAGAACA ATTGGACAAA GGGTCATTAT CGTTATTGTG CTGCTCTTTC TATGCTGGGG
    GAATATGACT GGGCCCTGCA AGCAAACATA AAAGCTCAAA AACTCTGTAA AAATGACCCT
    GAGGGAATCA AGGATCTAAT TCAGCAGCAT GTAAAGTTAC AAAAACAAAT AGAAGACCTG
    CAAGGTCAAA CACCAGCTAG TAATCCAGTC AAAGCCTTCT GTGAAAGCAG GGCCTACATA
    CCTAGTTTAT CAGCACCTGC ATTTAGTACT TCACTTAACT TTGTGGAAAC TGAAAAAAAT
    TTCAGAAAAA CTAACCACGA AATGGCAAAC GGTGGTAATC AGAATCCAAA GGTGGTAGAT
    GAAGCTCTGA AAGTAGATGA TTGTGACTGT CATCCTGAAT TTGTACCGCC ATCAAGTCAG
    CCTCCAAAAT ATAAAGGGAA ACAAAAATCT CGAAACAATG AATTGGAGAA GTTCAGCCCT
    GGTTCACAAG AAACTTTACC AGTAGATTTA AAAAACATCT TGGAGAAACA GTTTTCTAAA
    TCTTCCAGAG CCGCACACCA GGATTTTGCT AATATAATGA AAATGCTGAG AAGTTTAATT
    CAGGATGGCT ACACGGCCTT GCTGGAGCAG CGCTGCCGCA GCGCCGCCCA AGCCTTCACA
    GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA ATAAAGCAAT TGAACCTGGC CATGATTAAC
    TATGTGTTAG TAGTCTACGG ACTTGCCATT TCACTCCTTG GAATAGGACA GCCCGAGGAG
    TTATCTGAAG CTGAAAACCA GTTCAAGAGG ATGATTGAAC ACTACCCCAA TGAGGGGCTT
    GATTGCTTGG CCTACTGTGG AATTGGAAAA GTGTATTTGA AAAAAAACAG ATTTTTAGAA
    GCTCTTAATC ATTTTGAGAA AGCAAGAACC TTGATCTGCC GTCTTCCTGG AGTGTTAACT
    TGGCCCACAA GTAATGTGGT TATTGAGGAG ACTCAGCCAG GGAAGATAAA GATGCTGTTA
    GAGAAATTTG TTGAAGAATG CAGGTTCCCT CCAGTGCCGG ATGCCACTTG TTGCTATCAG
    AAGTGCCATG GATATTCCAA AATCCAGATA TATATAACTG ATCCAGACTT TAAGGGTTTT
    ATACGGATCA GCTGTTGCCA GTATTGTAAA ATAGAATTTC ACATGAACTG CTGGAAGAAG
    TTAAAAACAA CAACCTTCAA TGATAAAATT GACAAGGATT TTCTGCAAGG AATCTGTCTT
    ACTCCTGATT GTGAAGGAAT CATTTCAAAG ATTATCATCT TCAGCAGTGG TGGTCAAGTT
    AAATGTGAAT TTGAACACAA GGTCATTAAA GAAAAGGTTC CTCCAAGACC TATTCTGAAA
    CAGAAATGTT CTAGCCTAGA GAAGTTGAGG CTGAAAGAAG ACAAAAAATT GAAGCGAAAG
    CTCCAAAAAA AGGAAGCAAA AAAATTAGCA CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA
    AGTAACCCAC CAAAAACTGA AGAGCAGAGA GAAACTATCA ACCATGTCCA AAGTTGCCAG
    TTCCTTGATG ACAGAATTCT GCAGTGCATA AAGCAGTACG CTGACAAGAT TAAGTCCGGG
    ATACTGAATA CTTCCAAGCT TCTCAAAGAA TTGCTTTCTT GGAAGGTTTT AACCACAGAA
    GACTACACAA CGTGTTTTTC TAGCAGAAAT TTCCTGAATG AAGCAGTGGA CTATGTCATT
    CGTCATTTGA TCCAAGAAAA GAATAGAGTA AAGACAAGGG TATTTCTGCA TGTTTTGAGT
    GAGCTTAAAG AAGTGGACCC AAAACTAGCC ACCTGGATCC AGAAACTGAA TAGCTTTGGC
    TTAGATGCCA CAGGACCTTT CTTTTCTCGT TATGGGGCTC CTCTTAAGGA GCTTGATTTT
    AGCATCATGA CTTTTCTCTG GAATGAGAAA TACGGTCATA AGCTAGGCTC TATAGAAGGA
    AAGCAACTTG ATTATTTCTA TGAGCCTACA TCACTGAAGG AAGCGCGGTG TTTAATATGG
    CTGCTAGAAG AACACAGAGA CAAGTTCCCA GCACTGCATA ATGCCTTGGA CGAGTTCTTT
    GATATAATGG ACAGCCGCTG CACTGTATTA AGGAAACAGG ACAGTGGTGA CGTGCCATTT
    AATTCTACCA AGATAAAAAA CAAAGGCAAG AAAAAGAAGC CAAAAGATTC AAAGCCTATG
    TTAGTTGGAT CTGGAACACC TTCAGTCACA CCAAACAATG AGACTGTCAC TTCAGGTGAA
    GACCATATCA AACGCAGTTT AAGTTCTGCG GGCCCCTTTG TAGTGCCTGA TCACCTTCGG
    CAGGATATAG AAGAATTTGA AGCTCTGTAT GAACAGCATA GTAATGAGTA TGTTGTCCGT
    AATAAGAAGC TGTGGGACAT AAACCCAAAA CAGAAATGTT CAACTCTATA CGATTATTTC
    TCTCAGTTGT TGGAGGAACA TGGTCCCTTG GACATGAGTA ACAAAATGTT TTCTGAAGAA
    TATGAGTTCT TCCCAGAAGA AACTCAACAG ATACTAGAAA AAGCAGGAGG CTTGAAAGCG
    TTTCTCCTGG GATGTCCTCG TTTTGTTGTG ATTGATAACT GTATCGCATT GAAGAAAGTT
    GCATTACGGC TCAAGAAAAA GAGAAAGAAG AAAAACATTA AAACAAAAGT AGAAGAAATT
    TCAAAAACAG GAGAGTATTT ACGAGTTAAA CTACCACTGA ATCCAACTGC TAGGGAATTT
    AAACCAGATG TAAAGTCCAA ACCAGTGTCT GACTTACCTT CAGCACCAGC TTCTGAGGAC
    ATGAAGCCTC AGCCTGCACC TGCTAATTCT CCCCAATCAG CTCGTGACAC TGGGAAGCCA
    AAACCAGTTT TCGATAATTC TTCCAGATCA GTTTCTGAGG ATGAGAAGCC CAAAGGGATG
    TCTTCTGATT CTCCCAGGCC AGTCTTTGAG GATACCGGTC ACAAGCCGGT CTCCTCTAAT
    TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA GCGACCTATT GGCCTCGACC CCAGTTGGTC
    ACAGGATACT GTACATATCT TCCCTTTCGG GGATTTGACA TTACCCAGAC TCCACCAGCC
    TTCATCAGTG TGTTACCGAC TTTGCCCCAG TATAGCATTT ACACCCCTCT GGCCAGTGTT
    CCTACTGAGT ATCACCTGCA AAGACCAATA CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC
    ATAACGGAAG TGCATTTTGA GGCTCATCAT TTCAGTGCTG AGAATGCCCC TGGTACCCAG
    ATTGCCTCCG AAACACAGAT CCTTGAGGAA TCTTTGGAAA CACCAGTGAA GTCAGAGTGC
    AGCTATGATG GTGCTGATAT AGTCCAGAGT GAGGCTAGCA GAAACGATGA CTACTGTGGC
    GATTCTGCCA AATGGGAAGT AAATCCAGAA AGCACCAATG AAGTAACAAA TATTCCATGT
    ACACAAGTGG TTGCTGTACA GGTGTCTTGG AATATAACAC ATCAGGAAGT CAATACTGAG
    CCATATGGTC CTTTTGAGGC ACAACAGGGG GGTATTTCAC AGGTTGAAAA GGAGTACCAG
    GTTTTACAAG AGCAGCTTAA GGAAGCATGT GAAAATTATG AACACAGCAA TGTCAAGGGC
    TCAGAAGAAA CCAAGGACTT GGAAGAAAAG TTGAAAAGGA ATGTTGAAGA AAATAAGATC
    TCAAAGACAG AACTAGATTG GTTCCTTGAA GATTTGAACA AGGAAATTAA AAAATGGGAA
    CAGGAGAAAA AAGAAATCCA AGAAAGACTA AAGGCACTGA AGAAGAAAAT GAAAAAGGTG
    TTAAATGCCA GCAAAATGTA TACGCAGGAA AATGATGGAA AGGAAAAGGC ACTTGAATTA
    CTTCTGGATC AGTCCCTTGA AATCAGTAAT ACATTTACAA ATGAGAAAAT GAAAATAGAA
    GAGAGTATAA AGAAAGGGAA AGAGCATTAT GAAGAGAGTC TTCAAAGGGC TGTGGATGCA
    GAGGTATCTG TGCTTGAAAA CTGGAAGGAC ATGGAAGTGT GTAAGTTACA GAGCATGGAG
    TCACAAGCCG AAAGGTTTCT CAAGAATCTG AAGCTAATGA GCAGTGATTC GGCAGCGTAT
    TCTGACATGG ACTCAGACAT ACATTCATGG GAATCGTTTC TTTCTGATGT TAGAGAAGAA
    ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA CAAATTCAGG CAATTAAATA TGGTTCTCAG
    CTCAGTGAAC TTCCTAAAGT GCAGATTTCT GAGCTTTCAT TTCCTACCTG TAACACGATT
    CATCCTAAAT TACTTCCTGA GTCTTCAGGC CAGGATGGTC AAGGTCCCTC CACTTCTACA
    AGTCATGGGA CCGGAGACCA GACAGCAGCT CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT
    GACAGCCCAG TGGAGTTGCC CTCCTGCAGT CAGAAAGCCC CCCAGCTGCC AGAGCATGAG
    GGTGCTGGCC AGGCAACTGG GCCAGAACCA GGCCCAGTGG CTGATCAGAA GTTACCTGCT
    GCTCCAGAGC GTGCCACTCA TTCAAGCCAG TCTCCAAAGA AGCCGTTCAA TAGCATTATT
    GAGCACCTGT TGGTGGTGTT CCCGTGTTAC AGCAGCACGG AGCTTGCTGG TTTTATTAAA
    AAAGTTAGAA ACAAAAACAA GACCTCTCTG TCTGGATTGA GTATTGACGA AATTGTGCAA
    AGAGTGACAG AACACGTCCT TGATGAAGAA AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC
    TGGAGGCTGT CTGCACCCAG CTCTGCTGTT GCTGTGCCCA GGGCTGCCCA GGACCCGCCC
    TCCATGCCTG TCGGAGCATC GCCCGAAACC AGGGGGCCGA GGACCGAGGA CGTCCCTGTG
    TCCTGCGCAA ATTCCTGTGA AATGTGCCGA GAAGTATTCA AGTCCAAACA CGTGCGTGAG
    CTGCAGTGTG GGCACAGGCT TCACAAGGGG TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC
    ACCTGCCCAG CCTGCCTGGG TCACGACCTC CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa218652
    Clone ID Related Accession (Same CDS sequence) XM_024999711.1
    Accession Version XM_024999711.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6015bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase TTC3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037328.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT
    GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC
    AGGGGGCCGA GGACCGAGGA CGTCCCTGTG TCCTGCGCAA ATTCCTGTGA AATGTGCCGA
    GAAGTATTCA AGTCCAAACA CGTGCGTGAG CTGCAGTGTG GGCACAGGCT TCACAAGGGG
    TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC ACCTGCCCAG CCTGCCTGGG TCACGACCTC
    CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024855479.1
    CDS2831..8845
    Translation

    Target ORF information:

    RefSeq Version XM_024999711.1
    Organism Bos taurus(cattle)
    Definition Bos taurus tetratricopeptide repeat domain 3 (TTC3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024999711.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    ATGGGACGAA AAAAAGCATT CTTGATGAAG GTGAAAAACA TCTGTGGGTG GTGTACCATG 
    GCCGATTTTC CCCAGGGAAA TTTTGCAGAT TATGCCTTGT TAGAAGATTG CCCTTATGTG
    GATGATTGTA TCTTTGCCCC TGAGTTTATG TCCAGTGATT ATGTTCGTGT GACTCAGCTT
    TACTGTGATG GAGTGGGTAG ACAGTATAAA GATTATGCCC AAACTGAGGG AAACTTAGAA
    TTTGACATCT GCACTCTGTG GTGTAGTCAA CCTATTTCTG TCCTGCAAGA TTACTGTGAT
    GCCATTAAAG TCCACATCTT CTGGCCACTT CTGTTTCAAC GTCAGCAGAG CTCTATAATA
    ACACGGATGC ACTCCTGTGT AAGGACCAGC GGTTCTCATG CTTGTGAGAT AAGTTTGAAG
    AAATTACAGC AGCTTGAACT GATGGAAGAT ATTGTGGATT TGGCAAAGAA AGTTGTGAAT
    GATACATTCT TTATCGGAGG CTTATTGAGA ATTGGCTATA AAATAGAGAA TAAAATCTTG
    GCAATGGAAG AAGCTTTGAA TTGGGTGAAA TACACAGGCG ATGTAACAAT TTTATCTAAA
    TTAGGAGCAG TTGATAATTG TTGGCGTATG TTAAGTATTT TCTTTACTGA ATACAAGTAT
    CACATAACTA AAGTTGTAAC AGAAAACTGC AGTTTGCTTG AAGAATTTAA AACTCAAAGT
    TGTGCGGAGT GTCTAGAGCA AGGAGAACTA ATGAAAATGA AAGGAAATGA AGAGTTTTCC
    AAAGAAAGAT ATGATATAGC TATTATCTAT TACACCAGAG CCATTGAATG TAGGCCTGAA
    AATCACCTGC TTTATGGTAA CCGAGCTCTT TGTTTTCTTC GTACTGGACA ATTCAGAAAT
    GCACTTGGTG ATGGAAAGAG AGCTACCATT CTGAAGAACA ATTGGACAAA GGGTCATTAT
    CGTTATTGTG CTGCTCTTTC TATGCTGGGG GAATATGACT GGGCCCTGCA AGCAAACATA
    AAAGCTCAAA AACTCTGTAA AAATGACCCT GAGGGAATCA AGGATCTAAT TCAGCAGCAT
    GTAAAGTTAC AAAAACAAAT AGAAGACCTG CAAGGTCAAA CACCAGCTAG TAATCCAGTC
    AAAGCCTTCT GTGAAAGCAG GGCCTACATA CCTAGTTTAT CAGCACCTGC ATTTAGTACT
    TCACTTAACT TTGTGGAAAC TGAAAAAAAT TTCAGAAAAA CTAACCACGA AATGGCAAAC
    GGTGGTAATC AGAATCCAAA GGTGGTAGAT GAAGCTCTGA AAGTAGATGA TTGTGACTGT
    CATCCTGAAT TTGTACCGCC ATCAAGTCAG CCTCCAAAAT ATAAAGGGAA ACAAAAATCT
    CGAAACAATG AATTGGAGAA GTTCAGCCCT GGTTCACAAG AAACTTTACC AGTAGATTTA
    AAAAACATCT TGGAGAAACA GTTTTCTAAA TCTTCCAGAG CCGCACACCA GGATTTTGCT
    AATATAATGA AAATGTTGAG AAGTTTAATT CAGGATGGCT ACACGGCCTT GCTGGAGCAG
    CGCTGCCGCA GCGCCGCCCA AGCCTTCACA GAGTTGCTCA ACGGTTTAGA TCCTCAAAAA
    ATAAAGCAAT TAAACCTGGC CATGATTAAC TACGTGTTAG TAGTCTACGG ACTTGCCATT
    TCACTCCTTG GAATAGGACA GCCCGAGGAG TTATCTGAAG CTGAAAACCA GTTCAAGAGG
    ATGATTGAAC ACTACCCCAA TGAGGGGCTT GATTGCTTGG CCTACTGTGG AATTGGAAAA
    GTGTATTTGA AAAAAAACAG ATTTTTAGAA GCTCTTAATC ATTTTGAGAA AGCAAGAACC
    TTGATCTGTC GTCTTCCTGG AGTGTTAACT TGGCCCACAA GTAATGTGGT TATTGAGGAG
    ACTCAGCCAG GGAAGATAAA GATGCTGTTA GAGAAATTTG TTGAAGAATG CAGGTTCCCT
    CCAGTGCCGG ATGCCACTTG TTGCTATCAG AAGTGCCATG GATATTCCAA AATCCAGATA
    TATATAACTG ATCCAGACTT TAAGGGTTTT ATACGGATCA GCTGTTGCCA GTATTGTAAA
    ATAGAATTTC ACATGAACTG CTGGAAGAAG TTAAAAACAA CAACCTTCAA TGATAAAATT
    GACAAGGATT TTCTGCAAGG AATCTGTCTT ACTCCTGATT GTGAAGGAAT CATTTCAAAG
    ATTATCATCT TCAGCAGTGG TGGTCAAGTT AAATGTGAAT TTGAACACAA GGTCATTAAA
    GAAAAGGTTC CTCCAAGACC TATTCTGAAA CAGAAATGTT CTAGCCTAGA GAAGTTGAGG
    CTGAAAGAAG ACAAAAAATT GAAGCGAAAG CTCCAAAAAA AGGAAGCAAA AAAATTAGCA
    CAAGAAAGAC TGGAAGAGGA CTTAAGGGAA AGTAACCCAC CAAAAACTGA AGAGCAGAGA
    GAAACTATCA ACCATGTCCA AAGTTGCCAG TTCCTTGATG ACAGAATTCT GCAGTGCATA
    AAGCAGTACG CTGACAAGAT TAAGTCCGGG ATACTGAATA CTTCCAAGCT TCTCAAAGAA
    TTGCTTTCTT GGAAGGTTTT AACCACAGAA GACTACACAA CGTGTTTTTC TAGCAGAAAT
    TTCCTGAATG AAGCAGTGGA CTATGTCATT CGTCATTTGA TCCAAGAAAA GAATAGAGTA
    AAGACAAGGG TATTTCTGCA TGTTTTGAGT GAGCTTAAAG AAGTGGACCC AAAACTAGCC
    ACCTGGATCC AGAAACTGAA TAGCTTTGGC TTAGATGCCA CAGGACCTTT CTTTTCTCGT
    TATGGGGCTC CTCTTAAGGA GCTTGATTTT AGCATCATGA CTTTTCTCTG GAATGAGAAA
    TACGGTCATA AGCTAGGCTC TATAGAAGGA AAGCAACTTG ATTATTTCTA TGAGCCTACA
    TCACTGAAGG AAGCGCGGTG TTTAATATGG CTGCTAGAAG AACACAGAGA CAAGTTCCCA
    GCACTGCATA ATGCCTTGGA CGAGTTCTTT GATATAATGG ACAGCCGCTG CACTGTATTA
    AGGAAACAGG ACAGTGGTGA CGTGCCATTT AATTCTACCA AGATAAAAAA CAAAGGCAAG
    AAAAAGAAGC CAAAAGATTC AAAGCCTATG TTAGTTGGAT CTGGAACACC TTCAGTCACA
    CCAAACAATG AGACTGTCAC TTCAGGTGAA GACCATATCA AACGCAGTTT AAGTTCTGCG
    GGCCCCTTTG TAGTGCCTGA TCACCTTCGG CAGGATATAG AAGAATTTGA AGCTCTGTAT
    GAACAGCATA GTAATGAGTA TGTTGTCCGT AATAAGAAGC TGTGGGACAT AAACCCAAAA
    CAGAAATGTT CAACTCTATA CGATTATTTC TCTCAGTTGT TGGAGGAACA TGGTCCCTTG
    GACATGAGTA ACAAAATGTT TTCTGAAGAA TATGAGTTCT TCCCAGAAGA AACTCAACAG
    ATACTAGAAA AAGCAGGAGG CTTGAAAGCG TTTCTCCTGG GATGTCCTCG TTTTGTTGTG
    ATTGATAACT GTATCGCATT GAAGAAAGTT GCATTACGGC TCAAGAAAAA GAGAAAGAAG
    AAAAACATTA AAACAAAAGT AGAAGAAATT TCAAAAACAG GAGAGTATTT ACGAGTTAAA
    CTACCACTGA ATCCAACTGC TAGGGAATTT AAACCAGATG TAAAGTCCAA ACCAGTGTCT
    GACTTACCTT CAGCACCAGC TTCTGAGGAC ATGAAGCCTC AGCCTGCACC TGCTAATTCT
    CCCCAATCAG CTCGTGACAC TGGGAAGCCA AAACCAGTTT TCGATAATTC TTCCAGATCA
    GTTTCTGAGG ATGAGAAGCC CAAAGGGATG TCTTCTGATT CTCCCAGGCC AGTCTTTGAG
    GATACCGGTC ACAAGCCGGT CTCCTCTAAT TCTCCCAAAC CAGTTCCTGA GGAAGTGAAA
    GCGACCTATT GGCCTCGACC CCAGTTGGTC ACAGGATACT GTACATATCT TCCCTTTCGG
    GGATTTGACA TTACCCAGAC TCCACCAGCC TTCATCAGTG TGTTACCGAC TTTGCCCCAG
    TATAGCATTT ACACCCCTCT GGCCAGTGTT CCTACTGAGT ATCACCTGCA AAGACCAATA
    CCAGTGGTGC CGTCTTTTGT AGCCAGTGAC ATAACGGAAG TGCATTTTGA GGCTCATCAT
    TTCAGTGCTG AGAATGCCCC TGGTACCCAG ATTGCCTCCG AAACACAGAT CCTTGAGGAA
    TCTTTGGAAA CACCAGTGAA GTCAGAGTGC AGCTATGATG GTGCTGATAT AGTCCAGAGT
    GAGGCTAGCA GAAACGATGA CTACTGTGGC GATTCTGCCA AATGGGAAGT AAATCCAGAA
    AGCACCAATG AAGTAACAAA TATTCCATGT ACACAAGTGG TTGCTGTACA GGTGTCTTGG
    AATATAACAC ATCAGGAAGT CAATACTGAG CCATATGGTC CTTTTGAGGC ACAACAGGGG
    GGTATTTCAC AGGTTGAAAA GGAGTACCAG GTTTTACAAG AGCAGCTTAA GGAAGCATGT
    GAAAATTATG AACACAGCAA TGTCAAGGGC TCAGAAGAAA CCAAGGACTT GGAAGAAAAG
    TTGAAAAGGA ATGTTGAAGA AAATAAGATC TCAAAGACAG AACTAGATTG GTTCCTTGAA
    GATTTGAACA AGGAAATTAA AAAATGGGAA CAGGAGAAAA AAGAAATCCA AGAAAGACTA
    AAGGCACTGA AGAAGAAAAT GAAAAAGGTG TTAAATGCCA GCAAAATGTA TACGCAGGAA
    AATGATGGAA AGGAAAAGGC ACTTGAATTA CTTCTGGATC AGTCCCTTGA AATCAGTAAT
    ACATTTACAA ATGAGAAAAT GAAAATAGAA GAGAGTATAA AGAAAGGGAA AGAGCATTAT
    GAAGAGAGTC TTCAAAGGGC TGTGGATGCA GAGGTATCTG TGCTTGAAAA CTGGAAGGAC
    ATGGAAGTGT GTAAGTTACA GAGCATGGAG TCACAAGCCG AAAGGTTTCT CAAGAATCTG
    AAGCTAATGA GCAGTGATTC GGCAGCGTAT TCTGACATGG ACTCAGACAT ACATTCATGG
    GAATCGTTTC TTTCTGATGT TAGAGAAGAA ATTGAGAAAG CAAAGTCTCA GTTTGAAGAA
    CAAATTCAGG CAATTAAATA TGGTTCTCAG CTCAGTGAAC TTCCTAAAGT GCAGATTTCT
    GAGCTTTCAT TTCCTACCTG TAACACGATT CATCCTAAAT TACTTCCTGA GTCTTCAGGC
    CAGGATGGTC AAGGTCCCTC CACTTCTACA AGTCATGGGA CCGGAGACCA GACAGCAGCT
    CCCGAGGAGT CAAGTCTGGG CTCTGCAGCT GACAGCCCAG TGGAGTTGCC CTCCTGCAGT
    CAGAAAGCCC CCCAGCTGCC AGAGCATGAG GGTGCTGGCC AGGCAACTGG GCCAGAACCA
    GGCCCAGTGG CTGATCAGAA GTTACCTGCT GCTCCAGAGC GTGCCACTCA TTCAAGCCAG
    TCTCCAAAGA AGCCGTTCAA TAGCATTATT GAGCACCTGT TGGTGGTGTT CCCGTGTTAC
    AGCAGCACGG AGCTTGCTGG TTTTATTAAA AAAGTTAGAA ACAAAAACAA GACCTCTCTG
    TCTGGATTGA GTATTGACGA AATTGTGCAA AGAGTGACAG AACACGTCCT TGATGAAGAA
    AGAAAGAAAA AGCCAAGTCC AGGAAAGGGC TGGAGGCTGT CTGCACCCAG CTCTGCTGTT
    GCTGTGCCCA GGGCTGCCCA GGACCCGCCC TCCATGCCTG TCGGAGCATC GCCCGAAACC
    AGGGGGCCGA GGACCGAGGA CGTCCCTGTG TCCTGCGCAA ATTCCTGTGA AATGTGCCGA
    GAAGTATTCA AGTCCAAACA CGTGCGTGAG CTGCAGTGTG GGCACAGGCT TCACAAGGGG
    TGCTTCAAGC AGTGGCTGAA GAGCCAGCGC ACCTGCCCAG CCTGCCTGGG TCACGACCTC
    CTGTCAGAGG AGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.