PLB1 cDNA ORF clone, Bos taurus(cattle)

The following PLB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PLB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa46575 XM_015473562.1
Latest version!
Bos taurus phospholipase B1 (PLB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46575 XM_015465417.1
Latest version!
Bos taurus phospholipase B1 (PLB1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa46574 XM_015465416.1
Latest version!
Bos taurus phospholipase B1 (PLB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa46574 XM_015473561.1
Latest version!
Bos taurus phospholipase B1 (PLB1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa46575
    Clone ID Related Accession (Same CDS sequence) XM_015465417.1 , XM_015473562.1
    Accession Version XM_015465417.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phospholipase B1, membrane-associated isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG GACAAAAATG TGTTCATCAC CTTCAGCCAG CCTGTGAACC CAACCCACTC
    CCAACCTTGT TTCTATGGGG AAATACATCC AGACGTACAA ACTTTGGGAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320903.1
    CDS130..3903
    Translation

    Target ORF information:

    RefSeq Version XM_015465417.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase B1 (PLB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465417.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG GACAAAAATG TGTTCATCAC CTTCAGCCAG CCTGTGAACC CAACCCACTC
    CCAACCTTGT TTCTATGGGG AAATACATCC AGACGTACAA ACTTTGGGAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46575
    Clone ID Related Accession (Same CDS sequence) XM_015465417.1 , XM_015473562.1
    Accession Version XM_015473562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3774bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phospholipase B1, membrane-associated isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104296.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG GACAAAAATG TGTTCATCAC CTTCAGCCAG CCTGTGAACC CAACCCACTC
    CCAACCTTGT TTCTATGGGG AAATACATCC AGACGTACAA ACTTTGGGAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329048.1
    CDS130..3903
    Translation

    Target ORF information:

    RefSeq Version XM_015473562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase B1 (PLB1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG GACAAAAATG TGTTCATCAC CTTCAGCCAG CCTGTGAACC CAACCCACTC
    CCAACCTTGT TTCTATGGGG AAATACATCC AGACGTACAA ACTTTGGGAG GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46574
    Clone ID Related Accession (Same CDS sequence) XM_015473561.1 , XM_015465416.1
    Accession Version XM_015473561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product phospholipase B1, membrane-associated
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037338.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG AGGCCCGCTC TGCTGAGGAG TATGTTAAGT ACATCCGACA GGCCCTGGAC
    ATCCTCTACG AGCAGCTTCC ACGGGCTTTC ATCAACGTGG TAGAGGTCAT GGAGCTGGCT
    GACCTGCACC AGGGTCAAGA TGGAAAATGT GTCATGCCAC TGCCAGCCCA GAGCAACTGC
    ACCTGCCTAC AAGGCTCCCA GGAGAACTTG CCAGAGATGC AAGAATTGAA GACGGTGAAC
    TGGAACTTCC AGAGTGCCCT GTCGACACTC TCCTACCAGT ACCTGCAGCG TGAGGACTTC
    GCCGTGGTCG TACAGCCTTT CTTCCAAAAC ACTCTTGTCC CCCTGAACAA GAGAGGGGGC
    GCTGACCTCA CCTTCTTCTC CGAGGACTGT TTCCACTTCT CAGAACGTGG GCACGCAGAG
    ATGGCCATCG CTCTCTGGAA TAACATGCTA GAACCCTTGG GCCATAAGAC CACCTCCAAC
    AACTTCACCT ACAGCCGAAC CAAGCTCAAG TGCCCCTCTG CTGAGAACCC CTATCTCCGC
    ACCCTGAGGA ACAGTCAGCT GCTCCAAGAC CAGGCTGACA CAGAACCCTC AGTGCTCTCC
    TGGGCCATGC CAGTGGCGGC AGGAGGTGGC CTGGTGGTTG GCATCGGTGC GGTGATGACC
    TGGAGAGCCC TAAGAGGCCA CCAGAAGGAA AACTCTCCCC TAAGTCTGAA CACCATGAGC
    TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329047.1
    CDS130..4515
    Translation

    Target ORF information:

    RefSeq Version XM_015473561.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase B1 (PLB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015473561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG AGGCCCGCTC TGCTGAGGAG TATGTTAAGT ACATCCGACA GGCCCTGGAC
    ATCCTCTACG AGCAGCTTCC ACGGGCTTTC ATCAACGTGG TAGAGGTCAT GGAGCTGGCT
    GACCTGCACC AGGGTCAAGA TGGAAAATGT GTCATGCCAC TGCCAGCCCA GAGCAACTGC
    ACCTGCCTAC AAGGCTCCCA GGAGAACTTG CCAGAGATGC AAGAATTGAA GACGGTGAAC
    TGGAACTTCC AGAGTGCCCT GTCGACACTC TCCTACCAGT ACCTGCAGCG TGAGGACTTC
    GCCGTGGTCG TACAGCCTTT CTTCCAAAAC ACTCTTGTCC CCCTGAACAA GAGAGGGGGC
    GCTGACCTCA CCTTCTTCTC CGAGGACTGT TTCCACTTCT CAGAACGTGG GCACGCAGAG
    ATGGCCATCG CTCTCTGGAA TAACATGCTA GAACCCTTGG GCCATAAGAC CACCTCCAAC
    AACTTCACCT ACAGCCGAAC CAAGCTCAAG TGCCCCTCTG CTGAGAACCC CTATCTCCGC
    ACCCTGAGGA ACAGTCAGCT GCTCCAAGAC CAGGCTGACA CAGAACCCTC AGTGCTCTCC
    TGGGCCATGC CAGTGGCGGC AGGAGGTGGC CTGGTGGTTG GCATCGGTGC GGTGATGACC
    TGGAGAGCCC TAAGAGGCCA CCAGAAGGAA AACTCTCCCC TAAGTCTGAA CACCATGAGC
    TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46574
    Clone ID Related Accession (Same CDS sequence) XM_015473561.1 , XM_015465416.1
    Accession Version XM_015465416.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4386bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product phospholipase B1, membrane-associated isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645908.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG AGGCCCGCTC TGCTGAGGAG TATGTTAAGT ACATCCGACA GGCCCTGGAC
    ATCCTCTACG AGCAGCTTCC ACGGGCTTTC ATCAACGTGG TAGAGGTCAT GGAGCTGGCT
    GACCTGCACC AGGGTCAAGA TGGAAAATGT GTCATGCCAC TGCCAGCCCA GAGCAACTGC
    ACCTGCCTAC AAGGCTCCCA GGAGAACTTG CCAGAGATGC AAGAATTGAA GACGGTGAAC
    TGGAACTTCC AGAGTGCCCT GTCGACACTC TCCTACCAGT ACCTGCAGCG TGAGGACTTC
    GCCGTGGTCG TACAGCCTTT CTTCCAAAAC ACTCTTGTCC CCCTGAACAA GAGAGGGGGC
    GCTGACCTCA CCTTCTTCTC CGAGGACTGT TTCCACTTCT CAGAACGTGG GCACGCAGAG
    ATGGCCATCG CTCTCTGGAA TAACATGCTA GAACCCTTGG GCCATAAGAC CACCTCCAAC
    AACTTCACCT ACAGCCGAAC CAAGCTCAAG TGCCCCTCTG CTGAGAACCC CTATCTCCGC
    ACCCTGAGGA ACAGTCAGCT GCTCCAAGAC CAGGCTGACA CAGAACCCTC AGTGCTCTCC
    TGGGCCATGC CAGTGGCGGC AGGAGGTGGC CTGGTGGTTG GCATCGGTGC GGTGATGACC
    TGGAGAGCCC TAAGAGGCCA CCAGAAGGAA AACTCTCCCC TAAGTCTGAA CACCATGAGC
    TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015320902.1
    CDS130..4515
    Translation

    Target ORF information:

    RefSeq Version XM_015465416.1
    Organism Bos taurus(cattle)
    Definition Bos taurus phospholipase B1 (PLB1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015465416.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGGGCTGC TGCCCTGCAC TTTCCTCCTG GGGCTGCTAC TGCTTCTGGG GCGAGGAACG 
    ACCCAGGCCC ATACTTCCCC TGGAGAGAAC ACATTGGAAG AGCAGCTGTG GCCAGAGGGG
    CAGAGCCTGA AGAGTTTTTC ATTCTTTTGC AACCCAGAGA ATGTGCCTTC TCCAGGATCC
    AGCGTGCCTT CTAACTCAGT TCACTCTCTG AGACCTTCTG ATATTAAATT TGTGGCAGCC
    ATTGGCAGCC CTCCAGACTC AGGGACGGAC GACCTGGAGG AACAAGAATG GACTGAAAAG
    CAGGAGCCGC AAGCGTGCAT GGGAGTGGTG ACTGTCCTTT CAGATGTCAT CAGATACTTC
    AGCCCATCTG TTCTGAAGCC CATATGCACT CTTGGGACGG AAGTGGTACA CCATGATGCT
    GCTGAAGATT TGTGGATTCA GGCCAAAGAC CTGGTTAGGA ACATGAAAGA GAACCAGCAA
    CTTGACTTTC AAAATGATTG GAAGCTCATC AACGTGTTCT TCAGTAATGC TAGCCAGTGT
    CGCCTGTGTT CCTCCACCCA ACAGCAAAGT CACGTGATGA GCAGCATGGA CCAGCTGACC
    AGGACGCTGG ACTACTTGCA CCAGGAGGTC CCCAAAGCAT TTGTGAACCT AGTCGATCTC
    TCTGAGGCTC TGAGGGGCTC ACACTGGCAT CAGAAGACTC TGCTTAGCCC CACGGCCCAG
    CCCTGCAGGT GCTCAGGGGA GACCTCGCGG CTGAGCAGTG TTGTGACCCA GTGGACTTAC
    CAGGAAACTT GGGAACGTCT CCTGGCCTCC AGCAAGTACA ACGAGCAGGA GTCCTTCGCA
    GTCGTCTTCC AGCCCTTCTT CTACGAGAGA GCCCTGCCAC CAACCTCGGG GGAGCAGCCA
    TTCCAGGATC CCACCGCTCT CGGCTTGACT CTCTGGAACA GCATGATGCA ACCAGCAGGA
    CAGAAAGATG AACCGTTCAG TGCAAAAGAG AGACGGCCTC TGACATGTCC CTCCCAGGAG
    AATCCCTATC TGTTCACCTA CAGGAACAGC AACTATCTGC CCAGGATGCT AAAGCCCCAG
    GTGAAGACTG AGGTGAGAGA TGGAGTGGAA ATCAGGTGCT CTGACAAGGA TCCCTCTGAC
    ACGGTTCCCA CCTCAGTTCA CAGGCTGAAG CTGGCTGACA TCAAGGTCAT CGGAGCCCTG
    GGCGACTCAC TCACGGCAGG TAACGGGGCC GGGTCCAAGC CTGGAAACGT CCTGGACGTC
    TTGACTCAGT ATCGAGGTCT GTCCTGGAGT GTGGGCGGCG ACCAGAACCT CAGCAGCGTC
    ATCACCCTGC CCAACATGCT CCGGGAATTC AACCCTTCCC TGAAGGGCTT CTCTGTTGGC
    AAGGGAAGCG AAAGCAGTTC TGGGGCCTAC CTAAACCAGG CCGTGCCGGG AGCCCAAGCT
    GAGGATTTAC CTGTTCAGGC CAGGAGGCTG GTGGACCTGA TGAAGAATGA TGTGAATATA
    AATTTTCAGG AAGACTGGAA GATAATAACC CTGTTTATAG GTGGCAATGA CCTCTGTGAT
    GTCTGTGATG ACCCGGTCCG CTTCTCTCCG CAGAACTTCG TGGACAACAT TGGGCAGGCT
    CTGGACATCC TCCACGCTGA GGTGCCTCGG GCCTTTGTGA ACCTTGTCAA GGTGCTGGAG
    ATCATCAACC TGAGGAAGCT GTACCAGGAT AAGAACGTCA GCTGCCCGAG GCTGATCCTC
    CGGAATCTGT GTCCCTGTGT CTTGAAGTAT GATGATAATG CAACAGAACT TGCCTCCCTC
    GTCGAAGTGA ATAAGAAGTA TCAGGAGGGG ACTCACCAAC TGGTTGAGAG TGGGCGTTAC
    GACACCAGGG AAGATTTTAC AGTGGTCGTG CAGCCGTTCC TCGAAAAAGT GGACATGCCC
    ATGACCCCGG AGGGGTTGCC TGACAACTCT TACTTTGCTC CTGACTGTTT CCACTTCAGT
    AGCAAGGCTC ATGCCCATGC AGCCAGTGCC CTGTGGAACA ACATGTTGCA ACCTGTTGGC
    CAGAAGACAA GACAGCATAA TTTTGAAGAC AAGATCAATA TCACATGTCC AGACCAGGCC
    CAGCCGTTTC TGAGCACCTA CAAGAACCTC GTGCAGGATC ACGGGAGCCA GCTGCTGTGC
    AGTGACAGAG CCCCTTCTGC CTCACCACCC ACCTCAGTGC ACACCCTGAG ACCTGCAGAC
    ATCCAAGTTG TGGCCGCTCT GGGGGATTCT CTGACCGCCG GCAATGGAAT TGGCTCCAAA
    CCGAATGACC TTTCTGATGT CACCACTCAG TACCGGGGGC TTTCGTATAG TTCAGGAGGG
    GACAGCTCCC TGGAGAATGT GACCACCTTA CCAAATATCC TTCGGAAGTT TAATGGGAAT
    CTCACAGGCT ATGCGGTGGG CACAGGAGAT GCCAATGAGA CAAATGCATT CCTCAATCAA
    GCTGTTCCTG GAGCTAAGGC TGAGGAACTT GTAAGCCAAG TCCAGACTCT GATACAGAAG
    ATGAAATCCG ACCATAGAGT AAATTTCCAT GAGGACTGGA AGGTCATCAC GGTGCTGATT
    GGAGGCAGTG ATTTATGTGA CTACTGCACG GACTCGAATC TTTATTCTGC AGACAACTTT
    TTTGACCATC TCCGCAATGC CTTGGACATC CTACATAGAG AGGTACCCCG GGCGCTGGTC
    AACCTTGTGG ACTTCATGAG TCCCAGTATC ATGAGGCAGG TGTTCCTGGG GAACCCCGAC
    AAGTGTCCTG TGCATCAGGC CAGCATTTTG TGCAATTGTG TTCTGACCCC GCGGGAGAGC
    TCCCAAGAGC TGGCCAAGTT GGAGTCCATC ACCCGAGCCT ACCAGAGCAG CATGCGTGAG
    CTGGTGGAGT CCGGTCGGTA CGAGACGCGG GAGGACTTCT CTGTGGTTCT GCAGCCCTTC
    TTCTACAACA TCCGGCTCCC TGTCCTGGCG GATGGGCGCC CAGATGTGTC TTTCTTCGCC
    CCAGACTGCC TCAACCCAAG TCAGAAATTC CACTCCCAGC TCTCCAGGGC CCTTTGGGTC
    AACATGCTTG AACCACTGGG AAGGAAAACA GATACCCTGG ATCTGACAGC ATACATACCC
    CTCTCCTGCC CTGCTCAGAT TGAGCCCTTC TTGCGAACCC CCCGGAACAG TAACTACACA
    TACCTGACCA CGCCCGCCAT TGAGAACTGG GGGAGTGACT TCTTGTGTAT GGAGCAGAAT
    CCTTCCAACA GTGTCCCCAC GTCAGTCCAT GAGCTCCGAC CAGCAGATAT CAAAGTGGTG
    GCTGCCCTGG GTGACTCGCT GACTACAGCA GTAGGAGCCC GACCCAGCAA CTCCAGTGAC
    CTCCCCACAT CCTGGAGAGG ACTCTCCTGG AGCATTGGAG GGGATGGCGA CTTAGGGACC
    CACACCACAC TTCCCAACAT TCTGAAGAAG TTCAACCCCA ACATCCTTGG CTTCTCCACC
    GGCACCCGAG AGGAGACAGC AGGGCTGAAT GTAGCCACAG AAGGGGCCAG GGCTCGGGAC
    ATGCCAGCCC AGGCCCGGGA CCTCGTGGAG CGAATGAAAA CCAGCCCTAA AATCAACCTA
    CAGAAAGACT GGAAGCTGAT CACGCTCTTC ATTGGGAGTA ACGACTTGTG CCATTACTGT
    GACAATGAGG AGGCCCGCTC TGCTGAGGAG TATGTTAAGT ACATCCGACA GGCCCTGGAC
    ATCCTCTACG AGCAGCTTCC ACGGGCTTTC ATCAACGTGG TAGAGGTCAT GGAGCTGGCT
    GACCTGCACC AGGGTCAAGA TGGAAAATGT GTCATGCCAC TGCCAGCCCA GAGCAACTGC
    ACCTGCCTAC AAGGCTCCCA GGAGAACTTG CCAGAGATGC AAGAATTGAA GACGGTGAAC
    TGGAACTTCC AGAGTGCCCT GTCGACACTC TCCTACCAGT ACCTGCAGCG TGAGGACTTC
    GCCGTGGTCG TACAGCCTTT CTTCCAAAAC ACTCTTGTCC CCCTGAACAA GAGAGGGGGC
    GCTGACCTCA CCTTCTTCTC CGAGGACTGT TTCCACTTCT CAGAACGTGG GCACGCAGAG
    ATGGCCATCG CTCTCTGGAA TAACATGCTA GAACCCTTGG GCCATAAGAC CACCTCCAAC
    AACTTCACCT ACAGCCGAAC CAAGCTCAAG TGCCCCTCTG CTGAGAACCC CTATCTCCGC
    ACCCTGAGGA ACAGTCAGCT GCTCCAAGAC CAGGCTGACA CAGAACCCTC AGTGCTCTCC
    TGGGCCATGC CAGTGGCGGC AGGAGGTGGC CTGGTGGTTG GCATCGGTGC GGTGATGACC
    TGGAGAGCCC TAAGAGGCCA CCAGAAGGAA AACTCTCCCC TAAGTCTGAA CACCATGAGC
    TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.