KNTC1 cDNA ORF clone, Bos taurus(cattle)

The following KNTC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KNTC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa27816 NM_001192091.2
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_024977090.1
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_010813889.3
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_005217760.4
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_024977089.1
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_010813890.1
Latest version!
Bos taurus kinetochore associated 1 (KNTC1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
View Old Accession Versions >>
OBa27816 XM_005217760.3 Bos taurus kinetochore associated 1 (KNTC1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
OBa27816 XM_010813889.2 Bos taurus kinetochore associated 1 (KNTC1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1161.30
$1659.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version NM_001192091.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000017.1. On Apr 14, 2011 this sequence version replaced NM_001192091.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001179020.2
    CDS42..6656
    Translation

    Target ORF information:

    RefSeq Version NM_001192091.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001192091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_005217760.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005217760.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217817.1
    CDS207..6821
    Translation

    Target ORF information:

    RefSeq Version XM_005217760.4
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217760.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_005217760.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005217760.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005217817.1
    CDS261..6875
    Translation

    Target ORF information:

    RefSeq Version XM_005217760.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005217760.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_024977089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832857.1
    CDS319..6933
    Translation

    Target ORF information:

    RefSeq Version XM_024977089.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_010813890.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812192.1
    CDS1714..8328
    Translation

    Target ORF information:

    RefSeq Version XM_010813890.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813890.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_024977090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832858.1
    CDS283..6897
    Translation

    Target ORF information:

    RefSeq Version XM_024977090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024977090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_010813889.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104457.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010813889.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812191.1
    CDS297..6911
    Translation

    Target ORF information:

    RefSeq Version XM_010813889.2
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813889.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa27816
    Clone ID Related Accession (Same CDS sequence) XM_005217760.4 , NM_001192091.2 , XM_005217760.3 , XM_024977089.1 , XM_010813890.1 , XM_024977090.1 , XM_010813889.2 , XM_010813889.3
    Accession Version XM_010813889.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product kinetochore-associated protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037344.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010813889.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010812191.1
    CDS243..6857
    Translation

    Target ORF information:

    RefSeq Version XM_010813889.3
    Organism Bos taurus(cattle)
    Definition Bos taurus kinetochore associated 1 (KNTC1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813889.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGTGGAATG ATATTGAGCT GCTAACAAAT GATGATACAG GAAGTGGGTA CCTAAGTGTT 
    GGTTCAGGAA AAGAACACGG AACCGCTTTA TATCAAGTAG ATTTACTAGC AAAGATCTCC
    TCTGAAAAGG CCTCATTAAA TCCAAAAATA CAAGCATGCA GCTTAAGTGA TGGGTTTATT
    ATTATAGCAG ACCAATCGGT GATACTGTTG GACAGTATTT GTAGATCTCT TCAGTTACAT
    CTTACATTTG ATACTGAAGT GGATGTAGTT GGCCTGTGTC AAGAAGGAAA GTTTCTTTTG
    GTTGGGGAGA GAAGTGGCAA CCTACATCTT ATTCATGTAA CGTCAAAGCT AACATTACTC
    ACCAATGCAT TTGTTCAGAA AGCTAACGAT GAAAATCAGT GTACTTACCG GAATCTTGTT
    ATTGAGAAAG ATAGTTCAAG CGAAGATACC TATTATATGC TGCTGCTTAC AAACAATGGA
    CTTTTTTATA TTACGAACCT TCAGCTTGTA AAAATTCAAC AAGCGATTGA AAAGGCAGAC
    TTCGATACAG CTAAGAAGTT ACAAGGACAA ATCAAGTCCA GCTTTATTTC TACTGAGAAT
    TATCATACTC TCGGTTGTCT CAATCTTGTG GCTGGTGATT TAGCAAGCGA AATCCCTGTG
    ATAATTGGGG GAACTGGCAA TTGTGCACTC TCAAAATGGG AGCCAGATTC TTCCAGGAAA
    GGGATGACAG TTAAGAGTGT TATTGATTCA GAGATTATCA AAGGTGCAAA GAAGTTCCAG
    CTAATAGACA ACCTACTTTT TGTTCTTGAT ACTGATAACG TGTTGAGCTT ATGGGATATT
    TACACCCTAA CTCCCATATG GAACTGGCCT GTCCTTCATG TAGAAGAGTT TCTTCTCACT
    ACAGAAGCAG AGTCACCTTC ATCAGTCACC TGGCAAGGGA TTACAAATCT CAAATTAGTG
    GCTCTAACAA CTACTACTAA TAAGAAGATG AAAAACCTTG TGGTCTGTTC ATTACCTGCG
    ATGGAAATAC TGTATTCTTT GGAAGTATCT GGTATTTCTT CTCTGGTGCA AACAGGAATT
    AGCACGGATA CCATATACCT TCTGGAAGGA ATTTTCAAAA ATGATCCAAG TTTGTCTGAA
    GACTCAGTCT CCGTTTTAGC ACTCAGATGT CTTACAGAGG CTTTACCAGA AAACAGATTG
    AGTCGGTTAC TTCACAAACA CAGATTTGCT GAAGCTGAGA GCTTTGCCAT TCAGTTTGGA
    CTAGATGTTG AGCTTGTTTA CAAAGTAAAA TCAAATGATA TATTGGAGAA ACTAGCTTTG
    AGTTCTCTGG ACACCAGTGA GCAGACTAAA TGGCAAAAAC TTGTAAATGA AGCCAAGGAA
    AATCTACATA AAATCCAGGA TGATGAGTTT GTGATGAATT ACTGCTTGGA GGCTCAGTGG
    ATAACCTATG AAACCACCCA TGAAATGCTA AATTATGCCA AAACCAGGCT TTTGAAGAAA
    GAAGATAAAA CTGTTCTTGT CTATTCTGAT GGCTTAAAAG AGGTACTAAG GGCTCATGCA
    AAATTGACTA CTTTTTATGG AGCATTTGGA CCAGAAAAAT TCAGTGGTAG TTCTTGGATT
    GAATTTCTGA ATAATGAAGA TGACCTTAAG GATATTTTTT TACAGCTAAG AGAAGGAAAC
    CTTGTTTGTG CACAGTACCT CTGGCTGAGA CATCGGGCAA ACTTTGAAAG CAGATTTGAT
    GTGAAAATGC TTGAGAACTT GCTCAACTCA ATTTCTACAC CAATCTCTCT GCAAAAGCTT
    TGTCCATGGC TTAAAAATGA TGTGATCCCA TTTGTGAGAA AGACTGTACC TGAAGGACAG
    AAAATTCTTG CAAAATGGTT GGAGCAAGCA GCCAGGAACC TTGAATTAAC TGATAAGGCA
    AACTGGCCAG AAAATGGGCT TCAACTGGCA GAGGTATTTT TTACAGCAGA AAATGAGTTG
    GGATTGGCAT CCTCTTGGAG TTGGAGTTCC TTGGATAATC AAAACATGGA GGAAGTATGT
    CAGTTAAGGA CTTTGGTAAA TAACCTTCGA GAGTTAATCA CACTGCATAG GAAATACAAC
    TGCAAATTAG CCCTCTCTGA TTTTGAGAAG GAGGATACAA CCACCATTGT ATTCCGAATG
    TTTGATAAAG TGCTGGCCCC CGAGCTTATT CCTTCCATCT TCCAGAAATT CATAAGAGTT
    TACATGCATG AACATGACTT ACAGGAGGAG GAACTTCTGC TTTTGTACAT AGAGGATTTA
    CTGAAGAGAT GTAGCTCAAA GTTCACCTCA CTCTTTGATA CAGCCTGGGA AGCAAAAGCC
    ATGGCAGTAA TAGGATGTTT ATCTGACACA GATCTCATAT TTGATGCTGT GCTCAAGATA
    ATGTATGCCG CAGTGGTCCC CTGGAGTGCA GCTGTGGAGC AGCTGGTGAA GCAGCACCTG
    GAGATGGGCC ATCCCAAGGT CAAGCTGTTA CAGGAAAGTT ACAAACTAAT GGAGATGAAA
    AAACTTTTAC GAGGCTATGG AATAAGGGAG GTGAATCTCT TAAACAAGGA AATAATGAGG
    GTGGTCAGAT ACATTCTCAA ACAAGATGTC CCATCTTCTT TAGAAGATGC TTTGAAGGTG
    GCTGAAGCAT ATATGTTGCC TAAAGATGAA ATCTACAGTC TAAGGATTAT TGACCTGATT
    GATAGAGAAC AGGGTGAAGA TTGTGTCCTT CTGTTGAGGT CTTTGCCTCT TGCTGAAGCT
    AAGAAAACTG CAGAAAGAGT AATCATATGG GCACGACTGG CTTTACAGGA AGAACCAGAT
    ACTTCTAATG AGGACAAGGC CTGGAGACTG TCTGTAGCGA AAACATCAGT AGACATTCTT
    AAAATACTTT GCAACAGTCA GAAAGATAAT CTGCAGAAGA AAGATGAATG GGAAGAAACC
    TTGAAACAGT TTAAAACTGT TGCTAGCTTA CAGGAGAACT TTGAGGTCTT TCTTTCATTT
    GAAGATTACA GCAAGAGTTC CCTGGTAGCA GAGCTCCGGG AGCAGCACAT TAAAGCTCAC
    CAAGTTGCAC AGGCCAAACA CAAACCCTGG AGGCCAGCAG AGCCCCGGGC CACTGAGGAG
    CAGAGGCTGA GCGCTGAGTC CAAGCTGCAC CGACAGGCGC TGACCCTGCA GGTGTCCAGG
    CAACAGCTGG AAGCCGAGCT GACCTTGAGG GCCTTGAGCG ATGGGAACGT GGAAGCTGCA
    CTGAGAAAAT GCAGGGACTT GTTTAAATAT CACTGCAACA CCGACACTGG GCAGTTCCTG
    TTCCTGATGT GTCAGAAGCT GTGTCACATG TTGGCCAGTG GCGTTCCAAT GACAGTACCT
    GTGGGTCTAA ATCTTCCCTC CGAGATACAT GACCTTGCAT GCCAGGCTGC CACCATCTGC
    AGTCCAGATT TTGTGCTGGA CGCCTTAGAA CTCTGTAAAT ACACTTTGAT GGCAGTAGAG
    CTTTCCAGAC AGTGCCAAAT GGATGATTGT GGAATTCTCA CGAAAACTTC TTTCGGAACT
    CATAAAGATC CATATGAGGA ATGGTCTTAC TGTGACTTCT TCAGTGAAGA TGGAATAGTT
    CTTGAGTCAC AGATGGTGCT CCCTGTCATC TATGAATTGA TTTCGTCACT TGTACCTCTG
    GCTGAAAGCA AGAGATATCC CTTGGATTCT GTGAGTTTAC CATACTGCTT CACCAGTGAA
    GGAGACAGCC TAATGTTACC TGTTATCAAT TCCATCTCTG CCTTGCTTCA GAACCTTCAG
    GAATCTAGCC AGTGGGAACT GGCCCTAAAA TTTGTGATTG GTTCCTTTGG TACCTGTCTT
    CAGCACTCTG TGTCAAGCTT CATGAATGCC AGTTTGAGCG AAAAGTTGTT TGAAGAGGCC
    CCTTTAGTTA AATCCAGGCA CGTTGTTATG GAATTGAAAG AGAAAGCTGT TATGTTTATC
    AGGGGAAATG CCACCACACT CTTGTACAAA GTATTTAATT GTCGCCTGGT GGATCTCGAC
    TTGGCACTGG GGTACTGCAC TCTCTTACCG CAAAAAGAAG TCTTTGAGAA TCTCTGGAAG
    CTCACAGATA AAGCATGGCA GAATTACGAC AAAATATTGG CAATATCTCT GGTGGGCTCT
    CAGTTGGCCA GTCTCTGTCA GGAAACAGAA ACGAAGTTTA AGTTCTGTGA ACTCAGTACT
    GATGCCCGGT GGGGTATTCG TCTTAGTAAA CTTGGTATTT CTTTCCAGCC AGTTTTCAGG
    CAACATTTTC TCACCAAGAA CGACCTCATC AAAGCTCTCG TAAAGAATAT AGATATGGAC
    ACAAGCATCA TTCTGGAATA TTGCAGCACG TTTCAGCTGG ATGCTGATGC GGCCCTTCAG
    CTGTTCATTG AAACACTCCT CCACAACACG AATGCCAGCC AGAGCCAGGG AGACGTGAGC
    ACAGGATCCA CAAAGCAGCA GCGTTCCAAA CTCCTGGCCA AAGCCCTCGA AATGGTTCCT
    TTATTGACGA GCACAAAAGA TTTGGTCATC AGTCTTAGTG GAATACTGCA TAAGTTGGAT
    CCCTATGACT ATGAGATGAT TGAAGTCGTC TTGAAAGTCA TAGAAAGAGC TGATGAGAAG
    ATAACCAACA TTAATATTAA TCAGGCACTA AGTCTTCTGA AACACTTGAA GTCATACAGA
    AGAATTTCTC CCCCAGTGGA CCTAGAACAT CAGTATATGT TGGAGCACGT CATAACTTTG
    CCATCAGCTG CCCAAACCAG ACTGCCTTTT CACCTAATAT TCTTTGGCAC AGCACAGAGC
    TTCTGGAAAA TTCTCTCTAC AGAACTCAGT GAAGAATCCT TCCCAACCTT GCTCTTAGTT
    TCCAAATTAA TGAAGTTCTC TTTGGACACT CTGTATGTGT CTACAGCCAA ACACGTTTTT
    GAAAAAAAGC TGAAGCCCAA GCTCCTGCAG TTAACACAGG GTAAATCCTC AACACTGATT
    AACAAGGAGA TAGCCAAGAT CACGCAAACC ATCGAATCCT ACCTGTTGTC CATAGTTAAC
    CCAGAGTGGG CCGTAGCCAT CGCCATCAGT CTTGCCCAGG ATGTCCCAGA AGGTTCCTTC
    AAGATGTCCA GTTTGAAATT CTGCCTATAT TTAGCTGAGA GGTGGCTTCA GAATATTCCA
    TCTCAGGATG AGACACGTGA AAAAGCTGAA GCTCTGCTGA AGAAGCTTCA TGTTCAGTAC
    CGGCGATCAG GCACGGAAGC TGTGCTCATA GCCCACAAGC TGAACACTGC TGAGTATTTA
    AGAGTGATCG GAAAACCAGT CCATCTCATT GTCAGTCTGT ACGAACATCC CAGCATAAAC
    CAAAGAATTC AGAATTCATG TGGGAAAGAT TATCCTGATA TTCACACAGC TGCTAAAGAA
    ATAGCTGAAG TCAATGAAAT TAATTTGGAA AAAGTCTGGG ACATGTTGTT GGAAAAATGG
    CTGTGCCCGT CCGCCAAACC TGATGAAACA CCATCAGAAT TGTTTGAACT TCAAGAAGAT
    GAAACACTAC GAAGAGTCCT GTATCTCCTT CTGTCTCGTC CAGTTGATTA TAGTTCAAGA
    ATGCTGTTCA TCTTTGCAAC ATCAGCCACA ACCATGCTCA GGATGCACCA GCTCACTTTC
    GCCCACAGAA CTCGAGCCCT GCAGTGTCTC CTCTACTTGG CTGACAAGGA AACTATAGAA
    TCTCTCTTCA AAAAATCCAT CGAAGAAGTG AAATCTTACT TGAAATGTAT AACTTTTCTG
    GCATCATTTG AGGCGCTGAA TATCCCCATC ACCTATGAAT TATTTTGCAA CAGCCCTAAA
    GAAGGCATGA TTAAGGGTCT TTGGAAAAAC CACAGCCACG AACCCATGGC AGTAAAGTTG
    GTGACTGAGC TTTGTTTAGA ATACAAAATC TATGACCTGC AGCTTTGGAA TGGACTCTTG
    CAAAAGCTTC TTGGCTTCAA TATGATTCCT TATCTAAGGA AGGTTTTAAC AGCCATTTCT
    AGCATCTATC CTCTGTGGCA GGTCCCCTAC TTCGGCAGAG CTTGGCAGTG TGTGGTTCAG
    ATACCACTGC TTTCAGCCTC TTGTCCTTTA AGCCCCAGTC AGCTGTCAGA TTGTTGTGAG
    AGTCTCATTG CTGTTCTAGA ATGTCCAGTT TCAGATGACC TCGACATGAT CAGAGTGGCC
    AGACAGTATG TCCAGTTAGA GCTTCCGGCT TTTGCATTAG CGTGTCTAAT GCTCATGCCC
    CACTCAGAAA AAAGGCACCA GGAAATAAAG AATTTTCTGA GCTCGTGTGA CCCTCAGATC
    ATTTTACAGC AATTAGAGGA GCATATGAAT ACTGGCCAGT TGGCAGGGTT TTCACATCAA
    ATTAGAAGTC TGGTGCTGAA AAATATCATA GATAAGAAGG AGTTTGGGAT TTTGGCAAAG
    ACAAAATACT TCCCAGTGTT GAAATCACAC ATGATGAATA CCAACAACAT CACTGAACTC
    GTAAACTATT TGGCAGATGA GTTAAGTTTA GATGACGCTT CAGTCTTTAT AACTGAATAT
    TACAAGCATT GTGGGAAGCC CATACCACCA GGCACCACTC CCTGTGAAAC TCTGAAGATG
    TTTAATGGAT CCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.