DGKA cDNA ORF clone, Bos taurus(cattle)

The following DGKA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa46527 XM_010805132.3
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa19393 NM_001077860.1
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19393 XM_024991483.1
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19393 XM_005206530.3
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19393 XM_024991484.1
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46525 XM_010805129.3
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46526 XM_010805130.3
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46526 XM_010805131.3
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa19392 XM_005206530.4
Latest version!
Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
View Old Accession Versions >>
OBa46527 XM_010805132.2 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa46525 XM_010805129.2 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46526 XM_010805130.2 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa46526 XM_010805131.2 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa46527
    Clone ID Related Accession (Same CDS sequence) XM_010805132.3 , XM_010805132.2
    Accession Version XM_010805132.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803434.1
    CDS507..2294
    Translation

    Target ORF information:

    RefSeq Version XM_010805132.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805132.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46527
    Clone ID Related Accession (Same CDS sequence) XM_010805132.3 , XM_010805132.2
    Accession Version XM_010805132.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803434.1
    CDS552..2339
    Translation

    Target ORF information:

    RefSeq Version XM_010805132.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805132.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19393
    Clone ID Related Accession (Same CDS sequence) XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
    Accession Version NM_001077860.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-18
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126521.1. On or before Nov 21, 2006 this sequence version replaced XM_582779.2, XM_879248.1, XM_879276.1, XM_879307.1. ##Evidence-Data-START## Transcript exon combination :: BC126521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001071328.1
    CDS129..2333
    Translation

    Target ORF information:

    RefSeq Version NM_001077860.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001077860.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19393
    Clone ID Related Accession (Same CDS sequence) XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
    Accession Version XM_024991483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847251.1
    CDS308..2512
    Translation

    Target ORF information:

    RefSeq Version XM_024991483.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19393
    Clone ID Related Accession (Same CDS sequence) XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
    Accession Version XM_024991484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024847252.1
    CDS147..2351
    Translation

    Target ORF information:

    RefSeq Version XM_024991484.1
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024991484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19393
    Clone ID Related Accession (Same CDS sequence) XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
    Accession Version XM_005206530.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206587.2
    CDS109..2313
    Translation

    Target ORF information:

    RefSeq Version XM_005206530.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206530.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46525
    Clone ID Related Accession (Same CDS sequence) XM_010805129.2 , XM_010805129.3
    Accession Version XM_010805129.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803431.1
    CDS40..2367
    Translation

    Target ORF information:

    RefSeq Version XM_010805129.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805129.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46525
    Clone ID Related Accession (Same CDS sequence) XM_010805129.2 , XM_010805129.3
    Accession Version XM_010805129.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2328bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803431.1
    CDS40..2367
    Translation

    Target ORF information:

    RefSeq Version XM_010805129.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805129.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46526
    Clone ID Related Accession (Same CDS sequence) XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
    Accession Version XM_010805130.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803432.1
    CDS182..2389
    Translation

    Target ORF information:

    RefSeq Version XM_010805130.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805130.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46526
    Clone ID Related Accession (Same CDS sequence) XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
    Accession Version XM_010805131.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803433.1
    CDS294..2501
    Translation

    Target ORF information:

    RefSeq Version XM_010805131.2
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805131.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46526
    Clone ID Related Accession (Same CDS sequence) XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
    Accession Version XM_010805130.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803432.1
    CDS203..2410
    Translation

    Target ORF information:

    RefSeq Version XM_010805130.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805130.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46526
    Clone ID Related Accession (Same CDS sequence) XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
    Accession Version XM_010805131.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803433.1
    CDS309..2516
    Translation

    Target ORF information:

    RefSeq Version XM_010805131.3
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805131.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC
    TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa19392
    Clone ID Related Accession (Same CDS sequence) XM_005206530.4
    Accession Version XM_005206530.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2325bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product diacylglycerol kinase alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206530.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005206587.3
    CDS110..2434
    Translation

    Target ORF information:

    RefSeq Version XM_005206530.4
    Organism Bos taurus(cattle)
    Definition Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005206530.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
    AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG
    ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG
    GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT
    GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC
    CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT
    GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC
    TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG
    TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG
    ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA
    GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC
    CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG
    GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG
    TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC
    GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT
    CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG
    TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG
    TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT
    GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT
    GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC
    ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG
    AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG
    AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC
    AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT
    CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG
    GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT
    CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT
    GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC
    AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA
    GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT
    GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG
    ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC
    ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT
    ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC
    CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT
    GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG
    TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC
    TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG
    GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.