DGKA gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010805130.2	XP_010803432.1	diacylglycerol kinase alpha isoform X3
XM_010805132.3	XP_010803434.1	diacylglycerol kinase alpha isoform X5
XM_010805131.2	XP_010803433.1	diacylglycerol kinase alpha isoform X3
XM_024991483.1	XP_024847251.1	diacylglycerol kinase alpha isoform X4
NM_001077860.1	NP_001071328.1	diacylglycerol kinase alpha
XM_010805132.2	XP_010803434.1	diacylglycerol kinase alpha isoform X4
XM_005206530.4	XP_005206587.3	diacylglycerol kinase alpha isoform X2
XM_024991484.1	XP_024847252.1	diacylglycerol kinase alpha isoform X4
XM_010805129.2	XP_010803431.1	diacylglycerol kinase alpha isoform X1
XM_005206530.3	XP_005206587.2	diacylglycerol kinase alpha isoform X2
XM_010805129.3	XP_010803431.1	diacylglycerol kinase alpha isoform X1
XM_010805130.3	XP_010803432.1	diacylglycerol kinase alpha isoform X3
XM_010805131.3	XP_010803433.1	diacylglycerol kinase alpha isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa46527	XM_010805132.3 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa19393	NM_001077860.1 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19393	XM_024991483.1 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19393	XM_005206530.3 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19393	XM_024991484.1 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46525	XM_010805129.3 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46526	XM_010805130.3 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46526	XM_010805131.3 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa19392	XM_005206530.4 Latest version!	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
OBa46527	XM_010805132.2	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
OBa46525	XM_010805129.2	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46526	XM_010805130.2	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa46526	XM_010805131.2	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

DGKA ( XM_010805132.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803434.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OBa46527
Clone ID Related Accession (Same CDS sequence)	XM_010805132.3 , XM_010805132.2
Accession Version	XM_010805132.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805132.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803434.1
CDS	507..2294
Translation	MMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805132.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805132.3

ORF Insert Sequence:

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805132.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803434.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa46527
Clone ID Related Accession (Same CDS sequence)	XM_010805132.3 , XM_010805132.2
Accession Version	XM_010805132.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1788bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805132.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803434.1
CDS	552..2339
Translation	MMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805132.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805132.2

ORF Insert Sequence:

ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( NM_001077860.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001071328.1 Bos taurus diacylglycerol kinase alpha (DGKA), mRNA.

Price & Availability↑

CloneID	OBa19393
Clone ID Related Accession (Same CDS sequence)	XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
Accession Version	NM_001077860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-18
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC126521.1. On or before Nov 21, 2006 this sequence version replaced XM_582779.2, XM_879248.1, XM_879276.1, XM_879307.1. ##Evidence-Data-START## Transcript exon combination :: BC126521.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001071328.1
CDS	129..2333
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	NM_001077860.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077860.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_024991483.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847251.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa19393
Clone ID Related Accession (Same CDS sequence)	XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
Accession Version	XM_024991483.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847251.1
CDS	308..2512
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_024991483.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991483.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_024991484.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024847252.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa19393
Clone ID Related Accession (Same CDS sequence)	XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
Accession Version	XM_024991484.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024847252.1
CDS	147..2351
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_024991484.1
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024991484.1

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_005206530.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206587.2 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa19393
Clone ID Related Accession (Same CDS sequence)	XM_024991483.1 , NM_001077860.1 , XM_024991484.1 , XM_005206530.3
Accession Version	XM_005206530.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206530.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206587.2
CDS	109..2313
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_005206530.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206530.3

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805129.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803431.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa46525
Clone ID Related Accession (Same CDS sequence)	XM_010805129.2 , XM_010805129.3
Accession Version	XM_010805129.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805129.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803431.1
CDS	40..2367
Translation	MGSWPGRRAAISNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805129.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805129.2

ORF Insert Sequence:

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805129.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803431.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa46525
Clone ID Related Accession (Same CDS sequence)	XM_010805129.2 , XM_010805129.3
Accession Version	XM_010805129.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2328bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805129.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803431.1
CDS	40..2367
Translation	MGSWPGRRAAISNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805129.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805129.3

ORF Insert Sequence:

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805130.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803432.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa46526
Clone ID Related Accession (Same CDS sequence)	XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
Accession Version	XM_010805130.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805130.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803432.1
CDS	182..2389
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805130.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805130.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805131.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803433.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa46526
Clone ID Related Accession (Same CDS sequence)	XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
Accession Version	XM_010805131.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805131.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803433.1
CDS	294..2501
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805131.2
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805131.2

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805130.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803432.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa46526
Clone ID Related Accession (Same CDS sequence)	XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
Accession Version	XM_010805130.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805130.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803432.1
CDS	203..2410
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805130.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805130.3

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_010805131.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803433.1 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa46526
Clone ID Related Accession (Same CDS sequence)	XM_010805130.2 , XM_010805131.2 , XM_010805130.3 , XM_010805131.3
Accession Version	XM_010805131.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805131.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803433.1
CDS	309..2516
Translation	MAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTVSIACACQKQPLGSMDPKFPFHGFFTSF

Target ORF information:

RefSeq Version	XM_010805131.3
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805131.3

ORF Insert Sequence:

ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AGTGAGTATT GCCTGTGCCT GCCAGAAACA ACCTCTGGGC 
TCTATGGATC CTAAATTTCC ATTCCATGGA TTCTTTACTT CCTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DGKA ( XM_005206530.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206587.3 Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

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CloneID	OBa19392
Clone ID Related Accession (Same CDS sequence)	XM_005206530.4
Accession Version	XM_005206530.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	diacylglycerol kinase alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206530.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206587.3
CDS	110..2434
Translation	MGSWPGRRAAISNCPWKQSSKAQKYYKQAAFVFILKKKRKMAKERGLISPSDFAQLQKYMEYSTKKVSDVLKLFEDGEMAEYLQGDAIGYEGFQQFLKIYLEVDNVPDHLSQALFQSFQTGYYIEDTVREDVVCLSDVSCYFSLLEGGRPEDKLEFTFKLYDTDRNGILDSSEVDRIIIQMMRMAEYLDWDVSELRPILQEMMKEIDYDGSGSVSLAEWLRAGATTVPLLVLLGLEMTLKDNGQHMWRPKRFPRPVYCNLCESSIGLGKQGLSCNLCKYIVHDQCAMKALPCEVSTYAKSRKDIGVQSHVWVRGGCESGRCDRCQKKIRIYHSLVGLHCVWCHLEIHDDCLPAMGHECDCGLLRDHILPPSSIYPSVLASGQERKTSKISQKTMDDLSLSTSEALRIDPVSNTHPLLVFVNPKSGGKQGERVLWKFQYLLNPRQVFNLLKDGPEPGLRFFRDVPDYRILVCGGDGTVGWILESIDKANLPFVPPVAVLPLGTGNDLARCLRWGGGYEGQNLGKILKDLETSKVVHMDRWSVEVIPQQTEEKSDPVPFQIINNYFSIGVDASIAHRFHIMREKYPEKFNSRMKNKLWYFEFATSESIFSTCKKLEESLTVEICGKPLDLSNLSLEGIAVLNIPSTHGGSNLWGDTKRPHGDIHGINQALGATAKVITDPDILKTCVPDLSDKRLEVVGLEGAIEIGQIYTKLKNAGHRLAKCSEITFHTTKTLPMQIDGEPWMQTPCTIKITHRNQMPMLVGPPPRSSNFFGFLC

Target ORF information:

RefSeq Version	XM_005206530.4
Organism	Bos taurus(cattle)
Definition	Bos taurus diacylglycerol kinase alpha (DGKA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206530.4

ORF Insert Sequence:

ATGGGGTCGT GGCCCGGGAG GCGTGCGGCC ATCTCAAACT GCCCTTGGAA GCAAAGTTCC 
AAGGCCCAGA AGTACTACAA ACAAGCTGCC TTTGTGTTCA TCCTTAAGAA AAAAAGAAAG 
ATGGCCAAGG AGAGGGGCCT AATAAGCCCC AGTGATTTTG CCCAGCTGCA AAAATACATG 
GAATACTCCA CCAAAAAGGT CAGTGATGTT CTGAAGCTCT TTGAGGATGG TGAAATGGCT 
GAATATCTCC AAGGAGATGC CATTGGATAC GAGGGGTTCC AGCAATTCCT GAAAATCTAC 
CTGGAGGTGG ATAATGTTCC AGATCACCTA AGCCAGGCAC TGTTTCAGTC CTTCCAGACT 
GGCTACTACA TAGAAGACAC TGTAAGAGAA GATGTGGTGT GTCTCAGTGA TGTCTCCTGC 
TACTTTTCTC TTCTGGAGGG TGGCCGGCCA GAAGACAAGC TAGAATTCAC CTTCAAGCTG 
TACGACACGG ACAGAAATGG GATCCTGGAC AGCTCAGAAG TGGACAGGAT CATCATACAG 
ATGATGCGAA TGGCTGAATA CCTGGATTGG GATGTGTCTG AGCTGAGGCC GATTCTTCAA 
GAGATGATGA AAGAGATTGA CTATGATGGC AGTGGCTCTG TGTCCCTGGC TGAGTGGCTC 
CGGGCTGGGG CCACCACCGT GCCACTGCTC GTGCTGCTGG GCCTGGAGAT GACCCTGAAG 
GACAATGGGC AGCACATGTG GAGACCCAAG AGATTCCCCC GACCAGTCTA CTGCAACCTG 
TGCGAGTCGA GCATTGGTCT TGGCAAACAG GGGCTGAGCT GTAACCTCTG TAAATACATC 
GTTCACGACC AATGTGCCAT GAAGGCCCTG CCCTGTGAAG TCAGCACCTA TGCCAAGTCT 
CGGAAGGACA TTGGTGTCCA ATCACACGTG TGGGTGCGAG GAGGCTGTGA ATCTGGACGG 
TGTGACCGCT GTCAGAAAAA GATCCGGATC TACCACAGTC TGGTTGGGCT GCATTGTGTG 
TGGTGCCACC TAGAGATCCA TGATGACTGC CTTCCAGCCA TGGGCCATGA GTGTGACTGT 
GGGCTGCTCC GAGATCACAT CCTGCCTCCG TCTTCCATCT ATCCCAGCGT CCTGGCCTCT 
GGACAGGAGC GTAAAACTAG CAAAATAAGC CAGAAGACCA TGGATGATTT AAGTTTGAGC 
ACTTCTGAGG CTCTGCGGAT TGATCCTGTT TCTAACACCC ACCCACTTCT GGTCTTTGTG 
AACCCCAAGA GTGGCGGGAA GCAAGGCGAG AGGGTGCTTT GGAAATTCCA GTATCTGCTG 
AACCCTCGAC AGGTGTTCAA CCTCCTAAAG GATGGTCCTG AGCCAGGACT CAGATTCTTC 
AGAGATGTTC CTGATTACAG GATTTTGGTG TGCGGCGGAG ATGGCACAGT AGGCTGGATT 
CTAGAGTCCA TTGACAAAGC CAACTTGCCT TTTGTGCCTC CTGTCGCTGT GTTGCCCCTG 
GGCACTGGAA ATGATCTGGC TCGTTGCCTA AGATGGGGAG GAGGGTATGA GGGACAGAAT 
CTGGGGAAGA TCCTCAAGGA TTTAGAGACA AGTAAGGTGG TACATATGGA TCGATGGTCT 
GTGGAGGTGA TACCCCAACA AACTGAAGAA AAGAGTGACC CAGTCCCCTT TCAAATCATC 
AATAACTACT TCTCCATCGG TGTGGATGCC TCTATTGCTC ACCGATTCCA CATTATGCGA 
GAGAAATATC CTGAGAAGTT CAACAGCAGA ATGAAGAACA AGCTATGGTA CTTCGAATTT 
GCCACTTCTG AATCCATCTT CTCAACGTGC AAAAAGCTGG AGGAATCTTT GACAGTTGAG 
ATCTGTGGGA AACCACTGGA TCTGAGCAAC CTGTCCCTAG AAGGCATTGC AGTGCTGAAC 
ATCCCAAGCA CACATGGTGG CTCCAATCTC TGGGGTGACA CCAAGAGACC ACATGGCGAT 
ATCCATGGGA TCAACCAGGC CTTAGGTGCT ACTGCCAAAG TCATCACCGA CCCTGATATC 
CTGAAGACCT GTGTACCAGA CCTAAGTGAC AAGCGGCTGG AAGTAGTGGG GCTGGAGGGT 
GCAATTGAGA TAGGCCAGAT CTATACCAAG CTCAAGAATG CTGGACATCG GCTGGCCAAG 
TGCTCTGAGA TCACCTTCCA CACCACAAAA ACCCTCCCCA TGCAAATTGA CGGAGAACCC 
TGGATGCAGA CACCTTGTAC AATCAAGATC ACCCACAGGA ACCAGATGCC TATGCTCGTG 
GGCCCACCCC CTCGCTCCTC CAATTTTTTT GGCTTCTTGT GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DGKA
Entrez Gene ID	506348
Full Name	diacylglycerol kinase alpha
General protein information	Preferred Names diacylglycerol kinase alpha Names diacylglycerol kinase alpha DAG kinase alpha DGK-alpha diacylglycerol kinase, alpha 80kDa diglyceride kinase alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	DGKA	NP_001336.2
	DGKA	XP_001112326.1
Canis lupus familiaris (dog)	DGKA	XP_005625534.1
Bos taurus (cattle)	DGKA	NP_001071328.1
Mus musculus (house mouse)	Dgka	NP_058091.2
Xenopus tropicalis (tropical clawed frog)	dgka	XP_002934577.1
Danio rerio (zebrafish)	dgka	XP_003199458.2
Rattus norvegicus (Norway rat)	Dgka	NP_542965.1
Pan troglodytes (chimpanzee)	DGKA	XP_001169946.1
Danio rerio (zebrafish)	dgkaa	NP_001038780.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DGKA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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