BOLA-DRA cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	BOLA-DRA
Entrez Gene ID	506214
Full Name	major histocompatibility complex, class II, DR alpha
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Genome	Chromosome: 23 Map Location: 23q21

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012677.1	NP_001012695.1	major histocompatibility complex, class II, DR alpha precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PATR-DRA	NP_001127811.1
Gallus gallus (chicken)	BLA	NP_001231990.1
Bos taurus (cattle)	BOLA-DRA	NP_001012695.1
Mus musculus (house mouse)	H2-Ea-ps	XP_003688998.1
Rattus norvegicus (Norway rat)	RT1-Da	NP_001008847.1
Homo sapiens (human)	HLA-DRA	NP_061984.2
	MAMU-DRA	NP_001127770.1
Canis lupus familiaris (dog)	DLA-DRA	NP_001011723.1

Gene Ontology
Bibliography

Related articles in PubMed

Greater expression of TLR2, TLR4, and IL6 due to negative energy balance is associated with lower expression of HLA-DRA and HLA-A in bovine blood neutrophils after intramammary mastitis challenge with Streptococcus uberis.
Moyes KM, Drackley JK, Morin DE, Loor JJ
Functional & integrative genomics10(1)53-61(2010 Mar)

Short communication: Identification of allelic variation at the bovine DRA locus by polymerase chain reaction-single strand conformational polymorphism.
Zhou H, Hickford JG, Fang Q, Byun SO
Journal of dairy science90(4)1943-6(2007 Apr)

Characterization of 954 bovine full-CDS cDNA sequences.
Harhay GP, Sonstegard TS, Keele JW, Heaton MP, Clawson ML, Snelling WM, Wiedmann RT, Van Tassell CP, Smith TP
BMC genomics6166(2005 Nov)

Structure of the BoLA-DRB3 gene and promoter.
Russell GC, Smith JA, Oliver RA
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics31(3)145-51(2004 Jun)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ALOX5AP, CPNE3, IL1R2, IL6, TLR2, TLR4, and THY1 were upregulated in blood polymorphonuclear cells in negative energy balance versus positive energy balance cows.
Title: Greater expression of TLR2, TLR4, and IL6 due to negative energy balance is associated with lower expression of HLA-DRA and HLA-A in bovine blood neutrophils after intramammary mastitis challenge with Streptococcus uberis.

Four unique SSCP patterns were detected among 384 cattle from New Zealand and sequence analysis revealed that these SSCP patterns represent 4 DRA alleles; and 3 single nucleotide polymorphisms were detected in exon 2
Title: Short communication: Identification of allelic variation at the bovine DRA locus by polymerase chain reaction-single strand conformational polymorphism.

The following BOLA-DRA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BOLA-DRA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa39905	NM_001012677.1 Latest version!	Bos taurus major histocompatibility complex, class II, DR alpha (BOLA-DRA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BOLA-DRA ( NM_001012677.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001012695.1 Bos taurus major histocompatibility complex, class II, DR alpha (BOLA-DRA), mRNA.

Price & Availability↑

CloneID	OBa39905
Clone ID Related Accession (Same CDS sequence)	NM_001012677.1
Accession Version	NM_001012677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	major histocompatibility complex, class II, DR alpha precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BT021059.1. On Aug 25, 2005 this sequence version replaced XM_582632.1. ##Evidence-Data-START## Transcript exon combination :: BT021059.1, BC142119.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN05861751, SAMN05861853 [ECO:0000348] ##Evidence-Data-END##

ATGGCCATAA CCAGGGTCCC AATATTAGGA CTTTTCATCA CTGTCCTGAT CGGCCTACAG 
GAATCGTGGG CTATTAAAGA GAATCATGTG ATCATCCAAG CTGAGTTCTA TCTGAAACCT 
GAGGAATCAG CCGAGTTTAT GTTTGACTTT GATGGTGATG AGATTTTCCA CGTGGATATG 
GGGAAGAAGG AGACGGTGTG GCGGCTTCCA GAATTTGGAC ATTTTGCCAG CTTTGAGGCT 
CAGGGTGCCC TGGCCAATAT GGCTGTGATG AAAGCCAACC TGGACATCAT GATAAAGCGC 
TCCAACAACA CCCCAAACAC CAATGTTCCT CCAGAAGTGA CTCTGCTCCC AAACAAGCCT 
GTGGAACTGG GAGAGCCCAA CACACTCATC TGCTTCATTG ACAAGTTCTC CCCACCCGTG 
ATCAGTGTCA CATGGCTTCG AAATGGCAAA CCTGTCACTG ATGGAGTGTC ACAGACGGTC 
TTCCTGCCCA GGAATGACCA CCTTTTCCGC AAGTTCCACT ACCTCCCCTT CCTGCCCACA 
ACAGAGGATG TCTATGACTG CAAGGTGGAG CACTTGGGTT TGAATGAGCC TCTTCTCAAG 
CACTGGGAGT ATGAAGCTCC AGCCCCCCTC CCAGAGACCA CAGAGAATGC AGTGTGTGCC 
CTGGGCCTGA TTGTGGCTCT GGTGGGCATC ATTGCAGGGA CCATCTTCAT CATCAAGGGC 
GTGCGCAAAG CCAACACCGT TGAACGCCGA GGGCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012695.1
CDS	84..845
Translation	MAITRVPILGLFITVLIGLQESWAIKENHVIIQAEFYLKPEESAEFMFDFDGDEIFHVDMGKKETVWRLPEFGHFASFEAQGALANMAVMKANLDIMIKRSNNTPNTNVPPEVTLLPNKPVELGEPNTLICFIDKFSPPVISVTWLRNGKPVTDGVSQTVFLPRNDHLFRKFHYLPFLPTTEDVYDCKVEHLGLNEPLLKHWEYEAPAPLPETTENAVCALGLIVALVGIIAGTIFIIKGVRKANTVERRGPL

Target ORF information:

RefSeq Version	NM_001012677.1
Organism	Bos taurus(cattle)
Definition	Bos taurus major histocompatibility complex, class II, DR alpha (BOLA-DRA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012677.1

ORF Insert Sequence:

ATGGCCATAA CCAGGGTCCC AATATTAGGA CTTTTCATCA CTGTCCTGAT CGGCCTACAG 
GAATCGTGGG CTATTAAAGA GAATCATGTG ATCATCCAAG CTGAGTTCTA TCTGAAACCT 
GAGGAATCAG CCGAGTTTAT GTTTGACTTT GATGGTGATG AGATTTTCCA CGTGGATATG 
GGGAAGAAGG AGACGGTGTG GCGGCTTCCA GAATTTGGAC ATTTTGCCAG CTTTGAGGCT 
CAGGGTGCCC TGGCCAATAT GGCTGTGATG AAAGCCAACC TGGACATCAT GATAAAGCGC 
TCCAACAACA CCCCAAACAC CAATGTTCCT CCAGAAGTGA CTCTGCTCCC AAACAAGCCT 
GTGGAACTGG GAGAGCCCAA CACACTCATC TGCTTCATTG ACAAGTTCTC CCCACCCGTG 
ATCAGTGTCA CATGGCTTCG AAATGGCAAA CCTGTCACTG ATGGAGTGTC ACAGACGGTC 
TTCCTGCCCA GGAATGACCA CCTTTTCCGC AAGTTCCACT ACCTCCCCTT CCTGCCCACA 
ACAGAGGATG TCTATGACTG CAAGGTGGAG CACTTGGGTT TGAATGAGCC TCTTCTCAAG 
CACTGGGAGT ATGAAGCTCC AGCCCCCCTC CCAGAGACCA CAGAGAATGC AGTGTGTGCC 
CTGGGCCTGA TTGTGGCTCT GGTGGGCATC ATTGCAGGGA CCATCTTCAT CATCAAGGGC 
GTGCGCAAAG CCAACACCGT TGAACGCCGA GGGCCTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Greater expression of TLR2, TLR4, and IL6 due to negative energy balance is associated with lower expression of HLA-DRA and HLA-A in bovine blood neutrophils after intramammary mastitis challenge with Streptococcus uberis.
Functional & integrative genomics10(1)53-61(2010 Mar)
Moyes KM,Drackley JK,Morin DE,Loor JJ

Short communication: Identification of allelic variation at the bovine DRA locus by polymerase chain reaction-single strand conformational polymorphism.
Journal of dairy science90(4)1943-6(2007 Apr)
Zhou H,Hickford JG,Fang Q,Byun SO

Characterization of 954 bovine full-CDS cDNA sequences.
BMC genomics6166(2005 Nov)
Harhay GP,Sonstegard TS,Keele JW,Heaton MP,Clawson ML,Snelling WM,Wiedmann RT,Van Tassell CP,Smith TP

Structure of the BoLA-DRB3 gene and promoter.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics31(3)145-51(2004 Jun)
Russell GC,Smith JA,Oliver RA

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

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BOLA-DRA cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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