ARHGEF2 cDNA ORF clone, Bos taurus(cattle)

The following ARHGEF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARHGEF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa39628 NM_001098881.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa17276 XM_005203665.4
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa17277 XM_024986260.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa17277 XM_005203666.4
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa17277 XM_024986265.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa46395 XM_005203664.4
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa88160 XM_015462085.2
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa17273 XM_024986243.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa232571 XM_024986248.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa232572 XM_024986249.1
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa88159 XM_005203664.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa46394 XM_010802880.3
Latest version!
Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OBa88160 XM_015462085.1 Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa46394 XM_010802880.2 Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa17276 XM_005203665.3 Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa17277 XM_005203666.3 Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa39628
    Clone ID Related Accession (Same CDS sequence) NM_001098881.2
    Accession Version NM_001098881.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAFC03006842.1 and AAFC03006841.1. On Dec 12, 2009 this sequence version replaced NM_001098881.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCTCGGA TCGAATCCCT CACACGGGCG CGGATCGACC GGAGCAGAGA GCTGGCCAGC 
    AAGACCCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CACGCTACAC CAATGGGCAC
    CTCTTCACCA CCATCTCCGT TTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC
    ACAGCCAAGG AAGCCCTCAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA
    GACACCCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAAGCTGC CCTGCTGAAG
    AACAACACTG CCTTGCAGTC CGTTTCCCTT CGCAGTAAGA CTACCACTCG GGAGCGGCCC
    AGCTCAGCCA TCTACCCCTC CGACATCCGG CAGTCCCTGC TCGGCTCCCG CCGTGGCCGC
    TCCTCCTTGT CTTTAGCCAA AAGTGTCTCC ACCACCAACA TTGCTGGACA CTTCAATGAT
    GAGTCTCCCC TGGGGCTTCG CCGGATCCTC TCACAGTCCA CGGACTCCCT CAACATGCGG
    AACCGGACAC TGTCGGTGGA GTCCCTCATC GACGAAGGTG CCGAGGTGAT CTACAACGAG
    CTGATGAGTG ACTTTGAGAT GGATGAGAAG GACTTTACAG CCGATTCCTG GAGCCTTGCA
    GTGGATAGCA GCTTCTTGCA GCAGCATAAA AAGGAGGTGA TGAAGCAGCA GGATGTCATC
    TATGAGCTGA TCCAGACAGA GCTACACCAC GTGCGGACGC TGAAGATCAT GACCCGCCTC
    TTCCGCACGG GCATGCTGGA AGAGCTGCAG CTGGAGCCGG GAGTGGTCCA GGGCCTGTTC
    CCCTGTGTGG ACGAGCTCAC TGACATCCAC ACGCGATTCC TCAGCCAGCT GCTGGATCGC
    CGGCGCCAGG CCCTGTGCCC TGGCAGCACC CGGAACTTCG TCATCCATCG CTTGGGGGAC
    CTGCTCATCA GCCAGTTCTC AGGTCCTAGC GCAGAGCAGA TGCGGAAGAC CTACTCAGAG
    TTCTGTAGCC GCCACACCAA GGCCTTAAAA CTCTATAAGG AGCTGTATGC CCGAGACAAA
    CGCTTCCAGC AGTTCATCCG GAAAGTGACC CGCTCGGCGG TGCTGAAGCG GCATGGGGTA
    CAAGAGTGCA TTCTGCTAGT GACTCAGCGC ATCACCAAGT ACCCGGTGCT CATCAACCGC
    ATCCTGCAGC ATTCCCACGG GATGGAGGAG GAGCGCCAGG ACCTGACCAA GGCCCTGGGG
    CTGGTGAAGG AGCTGCTGTC CAACGTGGAC CAGGATGTGC ACGAGCTGGA GAAAGGGGCC
    CGCCTGCAGG AGATCTACAA CCGCATGGAC CCTCGGGCCC AGGCCCCGGT TCCTGGCAAG
    GGCCCCTTTG GCCGAGAGGA GCTCCTGCGG CGCAAGCTCA TCCACGATGG TTGCCTGCTC
    TGGAAGACGG CGACTGGGCG CTTCAAAGAT GTGCTCATGC TGCTGATGAC AGATGTGCTG
    GTGTTTCTCC AAGAGAAGGA CCAGAAGTAC ATCTTTCCTG CCCTGGACAA GCCCTCGGTG
    GTGTCACTGC AGAATCTCAT CGTGCGGGAC ATCGCTAACC AGGAGAAAGG GATGTTTCTG
    ATCAGTGCGG CACCCCCTGA GATGTACGAG GTCCACACGG CATCCCGGGA TGACCGGAGC
    ACCTGGATCC GCGTCATTCA GCAGAGTGTG CGAGTATGCC CATCCAGGGA GGACTTCCCC
    CTGATTGAGA CAGAGGATGA GGCTTACCTC CGGCGTATCA AGATGGAGCT GCAGCAGAAA
    GACCGGGCAC TGGTGGAGCT GCTGCAGGAG AAGGTTGGGC TATTTGCCGA GATGACCCAC
    TTCCAGGTGG AAGAGGATGG CAGCAGCGGG GTGCCCCTGC CCACCCTGCC CAGGGGCCTC
    TTCCGCTCTG AGTCCCTTGA GTCCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT
    GAGGTGGAGG GCCTGAAAGA CCTGCTGGTA GGGCCTGGAG TGGAGCTGCT CTTGACACCC
    CGGGAACCAC CCTTGCCCAT GGAGCCAGAC AGCGGTGGTA ACACGAGTCC TGGAGTCACT
    GCCAACGGTG AGGCCAGAAC CTTCAATGGA TCCATTGAGC TCTGCAGAGC CGACTCAGAC
    TCCAGCCAGA AGGATCGAAA TGGAAATCAG CTGAGATCTC CCCAGGAGGA AGCGTTGCAG
    CGATTGGTCA ATCTCTATGG ACTTCTGCAT GGCCTACAGG CAGCTGTGGC CCAGCAGGAC
    ACTCTGATGG AAGCCCGGTT CCCTGAGGGC CCCGAGCGGC GGGAGAAGCT GACCAGAGCC
    AATTCCCGGG ATGGGGAGGC TGGCAGAGCC GGGGGTGTCC CTGCGGTTCC TGAAAAGCAG
    GCTACGGAAC TGGCGTTGCT GCAGCGGCAA CACGCACTAT TGCAGGAGGA GCTGCGACGC
    TGCCGACGGC TCGGCGAGGA GCGTGCGACT GAGGCCGGCA GCTTGGAAGC CCGGCTCCGG
    GAGAGCGAGC AGGCCCGTGC CCTGCTGGAG CGGGAGGCCG AGGAGGCTCG CAGGCAGCTG
    GCCGCCTTGG GCCACACGGA ACCCCCACTC GTGGCTGAGG CCCCCTGGGC CCGCCGGCCT
    CTGGATCCGC GGCGTCGGAG CCTCCCTGCT GGCGATGCCC TGTACTTGAG TTTCACACCC
    CCACAGCCCA GCCGAGGCCA CGACCGCCTG GATTTGCCTG TAACTATTCG CTCTGTCCAT
    CGACCCTTTG AAGACCGAGA GAGGCAGGAG CTGGGCAGCC CCGACGAGCG GCTGCAAGAT
    AGCAGTGACC CTGACACCGG CAGCGAGGAG GAGGGTAGCA GCCGTCTGTC TCCACCTCAT
    AGTCCACGAG ATTTCACCCG AATGCAGGAC ATCCCGGAAG AGACTGAGAG CCGAGATGGG
    GAGCCTGTAG CTTCTGAGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001092351.2
    CDS191..3154
    Translation

    Target ORF information:

    RefSeq Version NM_001098881.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor (GEF) 2 (ARHGEF2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098881.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTCTCGGA TCGAATCCCT CACACGGGCG CGGATCGACC GGAGCAGAGA GCTGGCCAGC 
    AAGACCCGGG AAAAGGAGAA GATGAAGGAA GCCAAGGACG CACGCTACAC CAATGGGCAC
    CTCTTCACCA CCATCTCCGT TTCGGGCATG ACCATGTGCT ATGCCTGTAA CAAGAGCATC
    ACAGCCAAGG AAGCCCTCAT CTGCCCAACC TGCAATGTGA CTATCCACAA CCGCTGTAAA
    GACACCCTCG CCAACTGTAC CAAGGTCAAG CAGAAGCAAC AGAAAGCTGC CCTGCTGAAG
    AACAACACTG CCTTGCAGTC CGTTTCCCTT CGCAGTAAGA CTACCACTCG GGAGCGGCCC
    AGCTCAGCCA TCTACCCCTC CGACATCCGG CAGTCCCTGC TCGGCTCCCG CCGTGGCCGC
    TCCTCCTTGT CTTTAGCCAA AAGTGTCTCC ACCACCAACA TTGCTGGACA CTTCAATGAT
    GAGTCTCCCC TGGGGCTTCG CCGGATCCTC TCACAGTCCA CGGACTCCCT CAACATGCGG
    AACCGGACAC TGTCGGTGGA GTCCCTCATC GACGAAGGTG CCGAGGTGAT CTACAACGAG
    CTGATGAGTG ACTTTGAGAT GGATGAGAAG GACTTTACAG CCGATTCCTG GAGCCTTGCA
    GTGGATAGCA GCTTCTTGCA GCAGCATAAA AAGGAGGTGA TGAAGCAGCA GGATGTCATC
    TATGAGCTGA TCCAGACAGA GCTACACCAC GTGCGGACGC TGAAGATCAT GACCCGCCTC
    TTCCGCACGG GCATGCTGGA AGAGCTGCAG CTGGAGCCGG GAGTGGTCCA GGGCCTGTTC
    CCCTGTGTGG ACGAGCTCAC TGACATCCAC ACGCGATTCC TCAGCCAGCT GCTGGATCGC
    CGGCGCCAGG CCCTGTGCCC TGGCAGCACC CGGAACTTCG TCATCCATCG CTTGGGGGAC
    CTGCTCATCA GCCAGTTCTC AGGTCCTAGC GCAGAGCAGA TGCGGAAGAC CTACTCAGAG
    TTCTGTAGCC GCCACACCAA GGCCTTAAAA CTCTATAAGG AGCTGTATGC CCGAGACAAA
    CGCTTCCAGC AGTTCATCCG GAAAGTGACC CGCTCGGCGG TGCTGAAGCG GCATGGGGTA
    CAAGAGTGCA TTCTGCTAGT GACTCAGCGC ATCACCAAGT ACCCGGTGCT CATCAACCGC
    ATCCTGCAGC ATTCCCACGG GATGGAGGAG GAGCGCCAGG ACCTGACCAA GGCCCTGGGG
    CTGGTGAAGG AGCTGCTGTC CAACGTGGAC CAGGATGTGC ACGAGCTGGA GAAAGGGGCC
    CGCCTGCAGG AGATCTACAA CCGCATGGAC CCTCGGGCCC AGGCCCCGGT TCCTGGCAAG
    GGCCCCTTTG GCCGAGAGGA GCTCCTGCGG CGCAAGCTCA TCCACGATGG TTGCCTGCTC
    TGGAAGACGG CGACTGGGCG CTTCAAAGAT GTGCTCATGC TGCTGATGAC AGATGTGCTG
    GTGTTTCTCC AAGAGAAGGA CCAGAAGTAC ATCTTTCCTG CCCTGGACAA GCCCTCGGTG
    GTGTCACTGC AGAATCTCAT CGTGCGGGAC ATCGCTAACC AGGAGAAAGG GATGTTTCTG
    ATCAGTGCGG CACCCCCTGA GATGTACGAG GTCCACACGG CATCCCGGGA TGACCGGAGC
    ACCTGGATCC GCGTCATTCA GCAGAGTGTG CGAGTATGCC CATCCAGGGA GGACTTCCCC
    CTGATTGAGA CAGAGGATGA GGCTTACCTC CGGCGTATCA AGATGGAGCT GCAGCAGAAA
    GACCGGGCAC TGGTGGAGCT GCTGCAGGAG AAGGTTGGGC TATTTGCCGA GATGACCCAC
    TTCCAGGTGG AAGAGGATGG CAGCAGCGGG GTGCCCCTGC CCACCCTGCC CAGGGGCCTC
    TTCCGCTCTG AGTCCCTTGA GTCCCCTCGT GGCGAGCGGC TGCTGCAGGA TGCCATCCGT
    GAGGTGGAGG GCCTGAAAGA CCTGCTGGTA GGGCCTGGAG TGGAGCTGCT CTTGACACCC
    CGGGAACCAC CCTTGCCCAT GGAGCCAGAC AGCGGTGGTA ACACGAGTCC TGGAGTCACT
    GCCAACGGTG AGGCCAGAAC CTTCAATGGA TCCATTGAGC TCTGCAGAGC CGACTCAGAC
    TCCAGCCAGA AGGATCGAAA TGGAAATCAG CTGAGATCTC CCCAGGAGGA AGCGTTGCAG
    CGATTGGTCA ATCTCTATGG ACTTCTGCAT GGCCTACAGG CAGCTGTGGC CCAGCAGGAC
    ACTCTGATGG AAGCCCGGTT CCCTGAGGGC CCCGAGCGGC GGGAGAAGCT GACCAGAGCC
    AATTCCCGGG ATGGGGAGGC TGGCAGAGCC GGGGGTGTCC CTGCGGTTCC TGAAAAGCAG
    GCTACGGAAC TGGCGTTGCT GCAGCGGCAA CACGCACTAT TGCAGGAGGA GCTGCGACGC
    TGCCGACGGC TCGGCGAGGA GCGTGCGACT GAGGCCGGCA GCTTGGAAGC CCGGCTCCGG
    GAGAGCGAGC AGGCCCGTGC CCTGCTGGAG CGGGAGGCCG AGGAGGCTCG CAGGCAGCTG
    GCCGCCTTGG GCCACACGGA ACCCCCACTC GTGGCTGAGG CCCCCTGGGC CCGCCGGCCT
    CTGGATCCGC GGCGTCGGAG CCTCCCTGCT GGCGATGCCC TGTACTTGAG TTTCACACCC
    CCACAGCCCA GCCGAGGCCA CGACCGCCTG GATTTGCCTG TAACTATTCG CTCTGTCCAT
    CGACCCTTTG AAGACCGAGA GAGGCAGGAG CTGGGCAGCC CCGACGAGCG GCTGCAAGAT
    AGCAGTGACC CTGACACCGG CAGCGAGGAG GAGGGTAGCA GCCGTCTGTC TCCACCTCAT
    AGTCCACGAG ATTTCACCCG AATGCAGGAC ATCCCGGAAG AGACTGAGAG CCGAGATGGG
    GAGCCTGTAG CTTCTGAGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17276
    Clone ID Related Accession (Same CDS sequence) XM_005203665.4 , XM_005203665.3
    Accession Version XM_005203665.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGACCGGAA AGGCCAAGAC CCGGGAAAAG GAGAAGATGA AGGAAGCCAA GGACGCACGC 
    TACACCAATG GGCACCTCTT CACCACCATC TCCGTTTCGG GCATGACCAT GTGCTATGCC
    TGTAACAAGA GCATCACAGC CAAGGAAGCC CTCATCTGCC CAACCTGCAA TGTGACTATC
    CACAACCGCT GTAAAGACAC CCTCGCCAAC TGTACCAAGG TCAAGCAGAA GCAACAGAAA
    GCTGCCCTGC TGAAGAACAA CACTGCCTTG CAGTCCGTTT CCCTTCGCAG TAAGACTACC
    ACTCGGGAGC GGCCCAGCTC AGCCATCTAC CCCTCCGACA TCCGGCAGTC CCTGCTCGGC
    TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA GCCAAAAGTG TCTCCACCAC CAACATTGCT
    GGACACTTCA ATGATGAGTC TCCCCTGGGG CTTCGCCGGA TCCTCTCACA GTCCACGGAC
    TCCCTCAACA TGCGGAACCG GACACTGTCG GTGGAGTCCC TCATCGACGA AGGTGCCGAG
    GTGATCTACA ACGAGCTGAT GAGTGACTTT GAGATGGATG AGAAGGACTT TACAGCCGAT
    TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC TTGCAGCAGC ATAAAAAGGA GGTGATGAAG
    CAGCAGGATG TCATCTATGA GCTGATCCAG ACAGAGCTAC ACCACGTGCG GACGCTGAAG
    ATCATGACCC GCCTCTTCCG CACGGGCATG CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG
    GTCCAGGGCC TGTTCCCCTG TGTGGATGAG CTCACTGACA TCCACACGCG CTTCCTCAGC
    CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG TGCCCTGGCA GCACCCGGAA CTTCGTCATC
    CATCGTTTGG GGGACCTGCT CATCAGCCAG TTCTCAGGTC CTAGCGCAGA GCAGATGCGG
    AAGACCTACT CAGAGTTCTG TAGCCGCCAC ACCAAGGCCT TAAAACTCTA TAAGGAGCTG
    TATGCCCGAG ACAAACGCTT CCAGCAGTTC ATCCGGAAAG TGACCCGCTC GGCGGTGCTG
    AAGCGGCATG GGGTACAAGA GTGCATTCTG CTAGTGACTC AGCGCATCAC CAAGTACCCG
    GTGCTCATCA ACCGCATCCT GCAGCATTCC CACGGGATGG AGGAGGAGCG CCAGGACCTG
    ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG CTGTCCAACG TGGACCAGGA TGTGCACGAG
    CTGGAGAAAG GGGCCCGCCT GCAGGAGATC TACAACCGCA TGGACCCTCG GGCCCAGGCC
    CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA GAGGAGCTCC TGCGGCGCAA GCTCATCCAC
    GATGGTTGCC TGCTCTGGAA GACGGCGACT GGGCGCTTCA AAGATGTGCT CATGCTGCTG
    ATGACAGATG TGCTGGTGTT TCTCCAAGAG AAGGACCAGA AGTACATCTT TCCTGCCCTG
    GACAAGCCCT CGGTGGTGTC ACTGCAGAAT CTCATCGTGC GGGACATCGC TAACCAGGAG
    AAAGGGATGT TTCTGATCAG TGCGGCACCC CCTGAGATGT ACGAGGTCCA CACGGCATCC
    CGGGATGACC GGAGCACCTG GATCCGCGTC ATTCAGCAGA GTGTGCGAGT ATGCCCATCC
    AGGGAGGACT TCCCCCTGAT TGAGACAGAG GATGAGGCTT ACCTCCGGCG TATCAAGATG
    GAGCTGCAGC AGAAAGACCG GGCACTGGTG GAGCTGCTGC AGGAGAAGGT TGGGCTATTT
    GCCGAGATGA CCCACTTCCA GGTGGAAGAG GATGGCAGCA GCGGGGTGCC CCTGCCCACC
    CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG
    CAGGATGCCA TCCGTGAGGT GGAGGGCCTG AAAGACCTGC TGGTAGGGCC TGGAGTGGAG
    CTGCTCTTGA CACCCCGGGA ACCACCCTTG CCCATGGAGC CAGACAGCGG TGGTAACACG
    AGTCCTGGAG TCACTGCCAA CGGTGAGGCC AGAACCTTCA ATGGATCCAT TGAGCTCTGC
    AGAGCCGACT CAGACTCCAG CCAGAAGGAT CGAAATGGAA ATCAGCTGAG ATCTCCCCAG
    GAGGAAGCGT TGCAGCGATT GGTCAATCTC TATGGACTTC TGCATGGCCT ACAGGCAGCT
    GTGGCCCAGC AGGACACTCT GATGGAAGCC CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG
    AAGCTGACCA GAGCCAATTC CCGGGATGGG GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG
    GTTCCTGAAA AGCAGGCTAC GGAACTGGCG TTGCTGCAGC GGCAACACGC ACTATTGCAG
    GAGGAGCTGC GACGCTGCCG ACGGCTCGGC GAGGAGCGTG CGACTGAGGC CGGCAGCTTG
    GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG
    GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC ACGGAACCCC CACTCGTGGC TGAGGCCCCC
    TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC
    TTGAGTTTCA CACCCCCACA GCCCAGCCGA GGCCACGACC GCCTGGATTT GCCTGTAACT
    ATTCGCTCTG TCCATCGACC CTTTGAAGAC CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC
    GAGCGGCTGC AAGATAGCAG TGACCCTGAC ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT
    CTGTCTCCAC CTCATAGTCC ACGAGATTTC ACCCGAATGC AGGACATCCC GGAAGAGACT
    GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203722.1
    CDS195..3113
    Translation

    Target ORF information:

    RefSeq Version XM_005203665.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203665.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGACCGGAA AGGCCAAGAC CCGGGAAAAG GAGAAGATGA AGGAAGCCAA GGACGCACGC 
    TACACCAATG GGCACCTCTT CACCACCATC TCCGTTTCGG GCATGACCAT GTGCTATGCC
    TGTAACAAGA GCATCACAGC CAAGGAAGCC CTCATCTGCC CAACCTGCAA TGTGACTATC
    CACAACCGCT GTAAAGACAC CCTCGCCAAC TGTACCAAGG TCAAGCAGAA GCAACAGAAA
    GCTGCCCTGC TGAAGAACAA CACTGCCTTG CAGTCCGTTT CCCTTCGCAG TAAGACTACC
    ACTCGGGAGC GGCCCAGCTC AGCCATCTAC CCCTCCGACA TCCGGCAGTC CCTGCTCGGC
    TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA GCCAAAAGTG TCTCCACCAC CAACATTGCT
    GGACACTTCA ATGATGAGTC TCCCCTGGGG CTTCGCCGGA TCCTCTCACA GTCCACGGAC
    TCCCTCAACA TGCGGAACCG GACACTGTCG GTGGAGTCCC TCATCGACGA AGGTGCCGAG
    GTGATCTACA ACGAGCTGAT GAGTGACTTT GAGATGGATG AGAAGGACTT TACAGCCGAT
    TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC TTGCAGCAGC ATAAAAAGGA GGTGATGAAG
    CAGCAGGATG TCATCTATGA GCTGATCCAG ACAGAGCTAC ACCACGTGCG GACGCTGAAG
    ATCATGACCC GCCTCTTCCG CACGGGCATG CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG
    GTCCAGGGCC TGTTCCCCTG TGTGGATGAG CTCACTGACA TCCACACGCG CTTCCTCAGC
    CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG TGCCCTGGCA GCACCCGGAA CTTCGTCATC
    CATCGTTTGG GGGACCTGCT CATCAGCCAG TTCTCAGGTC CTAGCGCAGA GCAGATGCGG
    AAGACCTACT CAGAGTTCTG TAGCCGCCAC ACCAAGGCCT TAAAACTCTA TAAGGAGCTG
    TATGCCCGAG ACAAACGCTT CCAGCAGTTC ATCCGGAAAG TGACCCGCTC GGCGGTGCTG
    AAGCGGCATG GGGTACAAGA GTGCATTCTG CTAGTGACTC AGCGCATCAC CAAGTACCCG
    GTGCTCATCA ACCGCATCCT GCAGCATTCC CACGGGATGG AGGAGGAGCG CCAGGACCTG
    ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG CTGTCCAACG TGGACCAGGA TGTGCACGAG
    CTGGAGAAAG GGGCCCGCCT GCAGGAGATC TACAACCGCA TGGACCCTCG GGCCCAGGCC
    CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA GAGGAGCTCC TGCGGCGCAA GCTCATCCAC
    GATGGTTGCC TGCTCTGGAA GACGGCGACT GGGCGCTTCA AAGATGTGCT CATGCTGCTG
    ATGACAGATG TGCTGGTGTT TCTCCAAGAG AAGGACCAGA AGTACATCTT TCCTGCCCTG
    GACAAGCCCT CGGTGGTGTC ACTGCAGAAT CTCATCGTGC GGGACATCGC TAACCAGGAG
    AAAGGGATGT TTCTGATCAG TGCGGCACCC CCTGAGATGT ACGAGGTCCA CACGGCATCC
    CGGGATGACC GGAGCACCTG GATCCGCGTC ATTCAGCAGA GTGTGCGAGT ATGCCCATCC
    AGGGAGGACT TCCCCCTGAT TGAGACAGAG GATGAGGCTT ACCTCCGGCG TATCAAGATG
    GAGCTGCAGC AGAAAGACCG GGCACTGGTG GAGCTGCTGC AGGAGAAGGT TGGGCTATTT
    GCCGAGATGA CCCACTTCCA GGTGGAAGAG GATGGCAGCA GCGGGGTGCC CCTGCCCACC
    CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG
    CAGGATGCCA TCCGTGAGGT GGAGGGCCTG AAAGACCTGC TGGTAGGGCC TGGAGTGGAG
    CTGCTCTTGA CACCCCGGGA ACCACCCTTG CCCATGGAGC CAGACAGCGG TGGTAACACG
    AGTCCTGGAG TCACTGCCAA CGGTGAGGCC AGAACCTTCA ATGGATCCAT TGAGCTCTGC
    AGAGCCGACT CAGACTCCAG CCAGAAGGAT CGAAATGGAA ATCAGCTGAG ATCTCCCCAG
    GAGGAAGCGT TGCAGCGATT GGTCAATCTC TATGGACTTC TGCATGGCCT ACAGGCAGCT
    GTGGCCCAGC AGGACACTCT GATGGAAGCC CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG
    AAGCTGACCA GAGCCAATTC CCGGGATGGG GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG
    GTTCCTGAAA AGCAGGCTAC GGAACTGGCG TTGCTGCAGC GGCAACACGC ACTATTGCAG
    GAGGAGCTGC GACGCTGCCG ACGGCTCGGC GAGGAGCGTG CGACTGAGGC CGGCAGCTTG
    GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG
    GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC ACGGAACCCC CACTCGTGGC TGAGGCCCCC
    TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC
    TTGAGTTTCA CACCCCCACA GCCCAGCCGA GGCCACGACC GCCTGGATTT GCCTGTAACT
    ATTCGCTCTG TCCATCGACC CTTTGAAGAC CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC
    GAGCGGCTGC AAGATAGCAG TGACCCTGAC ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT
    CTGTCTCCAC CTCATAGTCC ACGAGATTTC ACCCGAATGC AGGACATCCC GGAAGAGACT
    GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17276
    Clone ID Related Accession (Same CDS sequence) XM_005203665.4 , XM_005203665.3
    Accession Version XM_005203665.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2919bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGACCGGAA AGGCCAAGAC CCGGGAAAAG GAGAAGATGA AGGAAGCCAA GGACGCACGC 
    TACACCAATG GGCACCTCTT CACCACCATC TCCGTTTCGG GCATGACCAT GTGCTATGCC
    TGTAACAAGA GCATCACAGC CAAGGAAGCC CTCATCTGCC CAACCTGCAA TGTGACTATC
    CACAACCGCT GTAAAGACAC CCTCGCCAAC TGTACCAAGG TCAAGCAGAA GCAACAGAAA
    GCTGCCCTGC TGAAGAACAA CACTGCCTTG CAGTCCGTTT CCCTTCGCAG TAAGACTACC
    ACTCGGGAGC GGCCCAGCTC AGCCATCTAC CCCTCCGACA TCCGGCAGTC CCTGCTCGGC
    TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA GCCAAAAGTG TCTCCACCAC CAACATTGCT
    GGACACTTCA ATGATGAGTC TCCCCTGGGG CTTCGCCGGA TCCTCTCACA GTCCACGGAC
    TCCCTCAACA TGCGGAACCG GACACTGTCG GTGGAGTCCC TCATCGACGA AGGTGCCGAG
    GTGATCTACA ACGAGCTGAT GAGTGACTTT GAGATGGATG AGAAGGACTT TACAGCCGAT
    TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC TTGCAGCAGC ATAAAAAGGA GGTGATGAAG
    CAGCAGGATG TCATCTATGA GCTGATCCAG ACAGAGCTAC ACCACGTGCG GACGCTGAAG
    ATCATGACCC GCCTCTTCCG CACGGGCATG CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG
    GTCCAGGGCC TGTTCCCCTG TGTGGATGAG CTCACTGACA TCCACACGCG CTTCCTCAGC
    CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG TGCCCTGGCA GCACCCGGAA CTTCGTCATC
    CATCGTTTGG GGGACCTGCT CATCAGCCAG TTCTCAGGTC CTAGCGCAGA GCAGATGCGG
    AAGACCTACT CAGAGTTCTG TAGCCGCCAC ACCAAGGCCT TAAAACTCTA TAAGGAGCTG
    TATGCCCGAG ACAAACGCTT CCAGCAGTTC ATCCGGAAAG TGACCCGCTC GGCGGTGCTG
    AAGCGGCATG GGGTACAAGA GTGCATTCTG CTAGTGACTC AGCGCATCAC CAAGTACCCG
    GTGCTCATCA ACCGCATCCT GCAGCATTCC CACGGGATGG AGGAGGAGCG CCAGGACCTG
    ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG CTGTCCAACG TGGACCAGGA TGTGCACGAG
    CTGGAGAAAG GGGCCCGCCT GCAGGAGATC TACAACCGCA TGGACCCTCG GGCCCAGGCC
    CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA GAGGAGCTCC TGCGGCGCAA GCTCATCCAC
    GATGGTTGCC TGCTCTGGAA GACGGCGACT GGGCGCTTCA AAGATGTGCT CATGCTGCTG
    ATGACAGATG TGCTGGTGTT TCTCCAAGAG AAGGACCAGA AGTACATCTT TCCTGCCCTG
    GACAAGCCCT CGGTGGTGTC ACTGCAGAAT CTCATCGTGC GGGACATCGC TAACCAGGAG
    AAAGGGATGT TTCTGATCAG TGCGGCACCC CCTGAGATGT ACGAGGTCCA CACGGCATCC
    CGGGATGACC GGAGCACCTG GATCCGCGTC ATTCAGCAGA GTGTGCGAGT ATGCCCATCC
    AGGGAGGACT TCCCCCTGAT TGAGACAGAG GATGAGGCTT ACCTCCGGCG TATCAAGATG
    GAGCTGCAGC AGAAAGACCG GGCACTGGTG GAGCTGCTGC AGGAGAAGGT TGGGCTATTT
    GCCGAGATGA CCCACTTCCA GGTGGAAGAG GATGGCAGCA GCGGGGTGCC CCTGCCCACC
    CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG
    CAGGATGCCA TCCGTGAGGT GGAGGGCCTG AAAGACCTGC TGGTAGGGCC TGGAGTGGAG
    CTGCTCTTGA CACCCCGGGA ACCACCCTTG CCCATGGAGC CAGACAGCGG TGGTAACACG
    AGTCCTGGAG TCACTGCCAA CGGTGAGGCC AGAACCTTCA ATGGATCCAT TGAGCTCTGC
    AGAGCCGACT CAGACTCCAG CCAGAAGGAT CGAAATGGAA ATCAGCTGAG ATCTCCCCAG
    GAGGAAGCGT TGCAGCGATT GGTCAATCTC TATGGACTTC TGCATGGCCT ACAGGCAGCT
    GTGGCCCAGC AGGACACTCT GATGGAAGCC CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG
    AAGCTGACCA GAGCCAATTC CCGGGATGGG GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG
    GTTCCTGAAA AGCAGGCTAC GGAACTGGCG TTGCTGCAGC GGCAACACGC ACTATTGCAG
    GAGGAGCTGC GACGCTGCCG ACGGCTCGGC GAGGAGCGTG CGACTGAGGC CGGCAGCTTG
    GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG
    GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC ACGGAACCCC CACTCGTGGC TGAGGCCCCC
    TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC
    TTGAGTTTCA CACCCCCACA GCCCAGCCGA GGCCACGACC GCCTGGATTT GCCTGTAACT
    ATTCGCTCTG TCCATCGACC CTTTGAAGAC CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC
    GAGCGGCTGC AAGATAGCAG TGACCCTGAC ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT
    CTGTCTCCAC CTCATAGTCC ACGAGATTTC ACCCGAATGC AGGACATCCC GGAAGAGACT
    GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203722.1
    CDS193..3111
    Translation

    Target ORF information:

    RefSeq Version XM_005203665.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203665.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGACCGGAA AGGCCAAGAC CCGGGAAAAG GAGAAGATGA AGGAAGCCAA GGACGCACGC 
    TACACCAATG GGCACCTCTT CACCACCATC TCCGTTTCGG GCATGACCAT GTGCTATGCC
    TGTAACAAGA GCATCACAGC CAAGGAAGCC CTCATCTGCC CAACCTGCAA TGTGACTATC
    CACAACCGCT GTAAAGACAC CCTCGCCAAC TGTACCAAGG TCAAGCAGAA GCAACAGAAA
    GCTGCCCTGC TGAAGAACAA CACTGCCTTG CAGTCCGTTT CCCTTCGCAG TAAGACTACC
    ACTCGGGAGC GGCCCAGCTC AGCCATCTAC CCCTCCGACA TCCGGCAGTC CCTGCTCGGC
    TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA GCCAAAAGTG TCTCCACCAC CAACATTGCT
    GGACACTTCA ATGATGAGTC TCCCCTGGGG CTTCGCCGGA TCCTCTCACA GTCCACGGAC
    TCCCTCAACA TGCGGAACCG GACACTGTCG GTGGAGTCCC TCATCGACGA AGGTGCCGAG
    GTGATCTACA ACGAGCTGAT GAGTGACTTT GAGATGGATG AGAAGGACTT TACAGCCGAT
    TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC TTGCAGCAGC ATAAAAAGGA GGTGATGAAG
    CAGCAGGATG TCATCTATGA GCTGATCCAG ACAGAGCTAC ACCACGTGCG GACGCTGAAG
    ATCATGACCC GCCTCTTCCG CACGGGCATG CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG
    GTCCAGGGCC TGTTCCCCTG TGTGGATGAG CTCACTGACA TCCACACGCG CTTCCTCAGC
    CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG TGCCCTGGCA GCACCCGGAA CTTCGTCATC
    CATCGTTTGG GGGACCTGCT CATCAGCCAG TTCTCAGGTC CTAGCGCAGA GCAGATGCGG
    AAGACCTACT CAGAGTTCTG TAGCCGCCAC ACCAAGGCCT TAAAACTCTA TAAGGAGCTG
    TATGCCCGAG ACAAACGCTT CCAGCAGTTC ATCCGGAAAG TGACCCGCTC GGCGGTGCTG
    AAGCGGCATG GGGTACAAGA GTGCATTCTG CTAGTGACTC AGCGCATCAC CAAGTACCCG
    GTGCTCATCA ACCGCATCCT GCAGCATTCC CACGGGATGG AGGAGGAGCG CCAGGACCTG
    ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG CTGTCCAACG TGGACCAGGA TGTGCACGAG
    CTGGAGAAAG GGGCCCGCCT GCAGGAGATC TACAACCGCA TGGACCCTCG GGCCCAGGCC
    CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA GAGGAGCTCC TGCGGCGCAA GCTCATCCAC
    GATGGTTGCC TGCTCTGGAA GACGGCGACT GGGCGCTTCA AAGATGTGCT CATGCTGCTG
    ATGACAGATG TGCTGGTGTT TCTCCAAGAG AAGGACCAGA AGTACATCTT TCCTGCCCTG
    GACAAGCCCT CGGTGGTGTC ACTGCAGAAT CTCATCGTGC GGGACATCGC TAACCAGGAG
    AAAGGGATGT TTCTGATCAG TGCGGCACCC CCTGAGATGT ACGAGGTCCA CACGGCATCC
    CGGGATGACC GGAGCACCTG GATCCGCGTC ATTCAGCAGA GTGTGCGAGT ATGCCCATCC
    AGGGAGGACT TCCCCCTGAT TGAGACAGAG GATGAGGCTT ACCTCCGGCG TATCAAGATG
    GAGCTGCAGC AGAAAGACCG GGCACTGGTG GAGCTGCTGC AGGAGAAGGT TGGGCTATTT
    GCCGAGATGA CCCACTTCCA GGTGGAAGAG GATGGCAGCA GCGGGGTGCC CCTGCCCACC
    CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG
    CAGGATGCCA TCCGTGAGGT GGAGGGCCTG AAAGACCTGC TGGTAGGGCC TGGAGTGGAG
    CTGCTCTTGA CACCCCGGGA ACCACCCTTG CCCATGGAGC CAGACAGCGG TGGTAACACG
    AGTCCTGGAG TCACTGCCAA CGGTGAGGCC AGAACCTTCA ATGGATCCAT TGAGCTCTGC
    AGAGCCGACT CAGACTCCAG CCAGAAGGAT CGAAATGGAA ATCAGCTGAG ATCTCCCCAG
    GAGGAAGCGT TGCAGCGATT GGTCAATCTC TATGGACTTC TGCATGGCCT ACAGGCAGCT
    GTGGCCCAGC AGGACACTCT GATGGAAGCC CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG
    AAGCTGACCA GAGCCAATTC CCGGGATGGG GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG
    GTTCCTGAAA AGCAGGCTAC GGAACTGGCG TTGCTGCAGC GGCAACACGC ACTATTGCAG
    GAGGAGCTGC GACGCTGCCG ACGGCTCGGC GAGGAGCGTG CGACTGAGGC CGGCAGCTTG
    GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG
    GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC ACGGAACCCC CACTCGTGGC TGAGGCCCCC
    TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC
    TTGAGTTTCA CACCCCCACA GCCCAGCCGA GGCCACGACC GCCTGGATTT GCCTGTAACT
    ATTCGCTCTG TCCATCGACC CTTTGAAGAC CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC
    GAGCGGCTGC AAGATAGCAG TGACCCTGAC ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT
    CTGTCTCCAC CTCATAGTCC ACGAGATTTC ACCCGAATGC AGGACATCCC GGAAGAGACT
    GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17277
    Clone ID Related Accession (Same CDS sequence) XM_024986265.1 , XM_005203666.3 , XM_005203666.4 , XM_024986260.1
    Accession Version XM_024986265.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842033.1
    CDS41..2923
    Translation

    Target ORF information:

    RefSeq Version XM_024986265.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986265.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17277
    Clone ID Related Accession (Same CDS sequence) XM_024986265.1 , XM_005203666.3 , XM_005203666.4 , XM_024986260.1
    Accession Version XM_005203666.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203666.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203723.1
    CDS150..3032
    Translation

    Target ORF information:

    RefSeq Version XM_005203666.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203666.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17277
    Clone ID Related Accession (Same CDS sequence) XM_024986265.1 , XM_005203666.3 , XM_005203666.4 , XM_024986260.1
    Accession Version XM_005203666.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203666.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203723.1
    CDS163..3045
    Translation

    Target ORF information:

    RefSeq Version XM_005203666.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203666.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17277
    Clone ID Related Accession (Same CDS sequence) XM_024986265.1 , XM_005203666.3 , XM_005203666.4 , XM_024986260.1
    Accession Version XM_024986260.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2883bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842028.1
    CDS43..2925
    Translation

    Target ORF information:

    RefSeq Version XM_024986260.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986260.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAAGGAAG CCAAGGACGC ACGCTACACC AATGGGCACC TCTTCACCAC CATCTCCGTT 
    TCGGGCATGA CCATGTGCTA TGCCTGTAAC AAGAGCATCA CAGCCAAGGA AGCCCTCATC
    TGCCCAACCT GCAATGTGAC TATCCACAAC CGCTGTAAAG ACACCCTCGC CAACTGTACC
    AAGGTCAAGC AGAAGCAACA GAAAGCTGCC CTGCTGAAGA ACAACACTGC CTTGCAGTCC
    GTTTCCCTTC GCAGTAAGAC TACCACTCGG GAGCGGCCCA GCTCAGCCAT CTACCCCTCC
    GACATCCGGC AGTCCCTGCT CGGCTCCCGC CGTGGCCGCT CCTCCTTGTC TTTAGCCAAA
    AGTGTCTCCA CCACCAACAT TGCTGGACAC TTCAATGATG AGTCTCCCCT GGGGCTTCGC
    CGGATCCTCT CACAGTCCAC GGACTCCCTC AACATGCGGA ACCGGACACT GTCGGTGGAG
    TCCCTCATCG ACGAAGGTGC CGAGGTGATC TACAACGAGC TGATGAGTGA CTTTGAGATG
    GATGAGAAGG ACTTTACAGC CGATTCCTGG AGCCTTGCAG TGGATAGCAG CTTCTTGCAG
    CAGCATAAAA AGGAGGTGAT GAAGCAGCAG GATGTCATCT ATGAGCTGAT CCAGACAGAG
    CTACACCACG TGCGGACGCT GAAGATCATG ACCCGCCTCT TCCGCACGGG CATGCTGGAA
    GAGCTGCAGC TGGAGCCGGG AGTGGTCCAG GGCCTGTTCC CCTGTGTGGA TGAGCTCACT
    GACATCCACA CGCGCTTCCT CAGCCAGCTG CTGGATCGCC GGCGCCAGGC CCTGTGCCCT
    GGCAGCACCC GGAACTTCGT CATCCATCGT TTGGGGGACC TGCTCATCAG CCAGTTCTCA
    GGTCCTAGCG CAGAGCAGAT GCGGAAGACC TACTCAGAGT TCTGTAGCCG CCACACCAAG
    GCCTTAAAAC TCTATAAGGA GCTGTATGCC CGAGACAAAC GCTTCCAGCA GTTCATCCGG
    AAAGTGACCC GCTCGGCGGT GCTGAAGCGG CATGGGGTAC AAGAGTGCAT TCTGCTAGTG
    ACTCAGCGCA TCACCAAGTA CCCGGTGCTC ATCAACCGCA TCCTGCAGCA TTCCCACGGG
    ATGGAGGAGG AGCGCCAGGA CCTGACCAAG GCCCTGGGGC TGGTGAAGGA GCTGCTGTCC
    AACGTGGACC AGGATGTGCA CGAGCTGGAG AAAGGGGCCC GCCTGCAGGA GATCTACAAC
    CGCATGGACC CTCGGGCCCA GGCCCCGGTT CCTGGCAAGG GCCCCTTTGG CCGAGAGGAG
    CTCCTGCGGC GCAAGCTCAT CCACGATGGT TGCCTGCTCT GGAAGACGGC GACTGGGCGC
    TTCAAAGATG TGCTCATGCT GCTGATGACA GATGTGCTGG TGTTTCTCCA AGAGAAGGAC
    CAGAAGTACA TCTTTCCTGC CCTGGACAAG CCCTCGGTGG TGTCACTGCA GAATCTCATC
    GTGCGGGACA TCGCTAACCA GGAGAAAGGG ATGTTTCTGA TCAGTGCGGC ACCCCCTGAG
    ATGTACGAGG TCCACACGGC ATCCCGGGAT GACCGGAGCA CCTGGATCCG CGTCATTCAG
    CAGAGTGTGC GAGTATGCCC ATCCAGGGAG GACTTCCCCC TGATTGAGAC AGAGGATGAG
    GCTTACCTCC GGCGTATCAA GATGGAGCTG CAGCAGAAAG ACCGGGCACT GGTGGAGCTG
    CTGCAGGAGA AGGTTGGGCT ATTTGCCGAG ATGACCCACT TCCAGGTGGA AGAGGATGGC
    AGCAGCGGGG TGCCCCTGCC CACCCTGCCC AGGGGCCTCT TCCGCTCTGA GTCCCTTGAG
    TCCCCTCGTG GCGAGCGGCT GCTGCAGGAT GCCATCCGTG AGGTGGAGGG CCTGAAAGAC
    CTGCTGGTAG GGCCTGGAGT GGAGCTGCTC TTGACACCCC GGGAACCACC CTTGCCCATG
    GAGCCAGACA GCGGTGGTAA CACGAGTCCT GGAGTCACTG CCAACGGTGA GGCCAGAACC
    TTCAATGGAT CCATTGAGCT CTGCAGAGCC GACTCAGACT CCAGCCAGAA GGATCGAAAT
    GGAAATCAGC TGAGATCTCC CCAGGAGGAA GCGTTGCAGC GATTGGTCAA TCTCTATGGA
    CTTCTGCATG GCCTACAGGC AGCTGTGGCC CAGCAGGACA CTCTGATGGA AGCCCGGTTC
    CCTGAGGGCC CCGAGCGGCG GGAGAAGCTG ACCAGAGCCA ATTCCCGGGA TGGGGAGGCT
    GGCAGAGCCG GGGGTGTCCC TGCGGTTCCT GAAAAGCAGG CTACGGAACT GGCGTTGCTG
    CAGCGGCAAC ACGCACTATT GCAGGAGGAG CTGCGACGCT GCCGACGGCT CGGCGAGGAG
    CGTGCGACTG AGGCCGGCAG CTTGGAAGCC CGGCTCCGGG AGAGCGAGCA GGCCCGTGCC
    CTGCTGGAGC GGGAGGCCGA GGAGGCTCGC AGGCAGCTGG CCGCCTTGGG CCACACGGAA
    CCCCCACTCG TGGCTGAGGC CCCCTGGGCC CGCCGGCCTC TGGATCCGCG GCGTCGGAGC
    CTCCCTGCTG GCGATGCCCT GTACTTGAGT TTCACACCCC CACAGCCCAG CCGAGGCCAC
    GACCGCCTGG ATTTGCCTGT AACTATTCGC TCTGTCCATC GACCCTTTGA AGACCGAGAG
    AGGCAGGAGC TGGGCAGCCC CGACGAGCGG CTGCAAGATA GCAGTGACCC TGACACCGGC
    AGCGAGGAGG AGGGTAGCAG CCGTCTGTCT CCACCTCATA GTCCACGAGA TTTCACCCGA
    ATGCAGGACA TCCCGGAAGA GACTGAGAGC CGAGATGGGG AGCCTGTAGC TTCTGAGAGC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46395
    Clone ID Related Accession (Same CDS sequence) XM_005203664.4
    Accession Version XM_005203664.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005203664.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCGGCA ACAGGAGGCA ACCCAGCCGC AGGGGCCAGA CCCGGGAAAA GGAGAAGATG 
    AAGGAAGCCA AGGACGCACG CTACACCAAT GGGCACCTCT TCACCACCAT CTCCGTTTCG
    GGCATGACCA TGTGCTATGC CTGTAACAAG AGCATCACAG CCAAGGAAGC CCTCATCTGC
    CCAACCTGCA ATGTGACTAT CCACAACCGC TGTAAAGACA CCCTCGCCAA CTGTACCAAG
    GTCAAGCAGA AGCAACAGAA AGCTGCCCTG CTGAAGAACA ACACTGCCTT GCAGTCCGTT
    TCCCTTCGCA GTAAGACTAC CACTCGGGAG CGGCCCAGCT CAGCCATCTA CCCCTCCGAC
    ATCCGGCAGT CCCTGCTCGG CTCCCGCCGT GGCCGCTCCT CCTTGTCTTT AGCCAAAAGT
    GTCTCCACCA CCAACATTGC TGGACACTTC AATGATGAGT CTCCCCTGGG GCTTCGCCGG
    ATCCTCTCAC AGTCCACGGA CTCCCTCAAC ATGCGGAACC GGACACTGTC GGTGGAGTCC
    CTCATCGACG AAGGTGCCGA GGTGATCTAC AACGAGCTGA TGAGTGACTT TGAGATGGAT
    GAGAAGGACT TTACAGCCGA TTCCTGGAGC CTTGCAGTGG ATAGCAGCTT CTTGCAGCAG
    CATAAAAAGG AGGTGATGAA GCAGCAGGAT GTCATCTATG AGCTGATCCA GACAGAGCTA
    CACCACGTGC GGACGCTGAA GATCATGACC CGCCTCTTCC GCACGGGCAT GCTGGAAGAG
    CTGCAGCTGG AGCCGGGAGT GGTCCAGGGC CTGTTCCCCT GTGTGGATGA GCTCACTGAC
    ATCCACACGC GCTTCCTCAG CCAGCTGCTG GATCGCCGGC GCCAGGCCCT GTGCCCTGGC
    AGCACCCGGA ACTTCGTCAT CCATCGTTTG GGGGACCTGC TCATCAGCCA GTTCTCAGGT
    CCTAGCGCAG AGCAGATGCG GAAGACCTAC TCAGAGTTCT GTAGCCGCCA CACCAAGGCC
    TTAAAACTCT ATAAGGAGCT GTATGCCCGA GACAAACGCT TCCAGCAGTT CATCCGGAAA
    GTGACCCGCT CGGCGGTGCT GAAGCGGCAT GGGGTACAAG AGTGCATTCT GCTAGTGACT
    CAGCGCATCA CCAAGTACCC GGTGCTCATC AACCGCATCC TGCAGCATTC CCACGGGATG
    GAGGAGGAGC GCCAGGACCT GACCAAGGCC CTGGGGCTGG TGAAGGAGCT GCTGTCCAAC
    GTGGACCAGG ATGTGCACGA GCTGGAGAAA GGGGCCCGCC TGCAGGAGAT CTACAACCGC
    ATGGACCCTC GGGCCCAGGC CCCGGTTCCT GGCAAGGGCC CCTTTGGCCG AGAGGAGCTC
    CTGCGGCGCA AGCTCATCCA CGATGGTTGC CTGCTCTGGA AGACGGCGAC TGGGCGCTTC
    AAAGATGTGC TCATGCTGCT GATGACAGAT GTGCTGGTGT TTCTCCAAGA GAAGGACCAG
    AAGTACATCT TTCCTGCCCT GGACAAGCCC TCGGTGGTGT CACTGCAGAA TCTCATCGTG
    CGGGACATCG CTAACCAGGA GAAAGGGATG TTTCTGATCA GTGCGGCACC CCCTGAGATG
    TACGAGGTCC ACACGGCATC CCGGGATGAC CGGAGCACCT GGATCCGCGT CATTCAGCAG
    AGTGTGCGAG TATGCCCATC CAGGGAGGAC TTCCCCCTGA TTGAGACAGA GGATGAGGCT
    TACCTCCGGC GTATCAAGAT GGAGCTGCAG CAGAAAGACC GGGCACTGGT GGAGCTGCTG
    CAGGAGAAGG TTGGGCTATT TGCCGAGATG ACCCACTTCC AGGTGGAAGA GGATGGCAGC
    AGCGGGGTGC CCCTGCCCAC CCTGCCCAGG GGCCTCTTCC GCTCTGAGTC CCTTGAGTCC
    CCTCGTGGCG AGCGGCTGCT GCAGGATGCC ATCCGTGAGG TGGAGGGCCT GAAAGACCTG
    CTGGTAGGGC CTGGAGTGGA GCTGCTCTTG ACACCCCGGG AACCACCCTT GCCCATGGAG
    CCAGACAGCG GTGGTAACAC GAGTCCTGGA GTCACTGCCA ACGGTGAGGC CAGAACCTTC
    AATGGATCCA TTGAGCTCTG CAGAGCCGAC TCAGACTCCA GCCAGAAGGA TCGAAATGGA
    AATCAGCTGA GATCTCCCCA GGAGGAAGCG TTGCAGCGAT TGGTCAATCT CTATGGACTT
    CTGCATGGCC TACAGGCAGC TGTGGCCCAG CAGGACACTC TGATGGAAGC CCGGTTCCCT
    GAGGGCCCCG AGCGGCGGGA GAAGCTGACC AGAGCCAATT CCCGGGATGG GGAGGCTGGC
    AGAGCCGGGG GTGTCCCTGC GGTTCCTGAA AAGCAGGCTA CGGAACTGGC GTTGCTGCAG
    CGGCAACACG CACTATTGCA GGAGGAGCTG CGACGCTGCC GACGGCTCGG CGAGGAGCGT
    GCGACTGAGG CCGGCAGCTT GGAAGCCCGG CTCCGGGAGA GCGAGCAGGC CCGTGCCCTG
    CTGGAGCGGG AGGCCGAGGA GGCTCGCAGG CAGCTGGCCG CCTTGGGCCA CACGGAACCC
    CCACTCGTGG CTGAGGCCCC CTGGGCCCGC CGGCCTCTGG ATCCGCGGCG TCGGAGCCTC
    CCTGCTGGCG ATGCCCTGTA CTTGAGTTTC ACACCCCCAC AGCCCAGCCG AGGCCACGAC
    CGCCTGGATT TGCCTGTAAC TATTCGCTCT GTCCATCGAC CCTTTGAAGA CCGAGAGAGG
    CAGGAGCTGG GCAGCCCCGA CGAGCGGCTG CAAGATAGCA GTGACCCTGA CACCGGCAGC
    GAGGAGGAGG GTAGCAGCCG TCTGTCTCCA CCTCATAGTC CACGAGATTT CACCCGAATG
    CAGGACATCC CGGAAGAGAC TGAGAGCCGA GATGGGGAGC CTGTAGCTTC TGAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203721.4
    CDS100..3039
    Translation

    Target ORF information:

    RefSeq Version XM_005203664.4
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203664.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGCGGCA ACAGGAGGCA ACCCAGCCGC AGGGGCCAGA CCCGGGAAAA GGAGAAGATG 
    AAGGAAGCCA AGGACGCACG CTACACCAAT GGGCACCTCT TCACCACCAT CTCCGTTTCG
    GGCATGACCA TGTGCTATGC CTGTAACAAG AGCATCACAG CCAAGGAAGC CCTCATCTGC
    CCAACCTGCA ATGTGACTAT CCACAACCGC TGTAAAGACA CCCTCGCCAA CTGTACCAAG
    GTCAAGCAGA AGCAACAGAA AGCTGCCCTG CTGAAGAACA ACACTGCCTT GCAGTCCGTT
    TCCCTTCGCA GTAAGACTAC CACTCGGGAG CGGCCCAGCT CAGCCATCTA CCCCTCCGAC
    ATCCGGCAGT CCCTGCTCGG CTCCCGCCGT GGCCGCTCCT CCTTGTCTTT AGCCAAAAGT
    GTCTCCACCA CCAACATTGC TGGACACTTC AATGATGAGT CTCCCCTGGG GCTTCGCCGG
    ATCCTCTCAC AGTCCACGGA CTCCCTCAAC ATGCGGAACC GGACACTGTC GGTGGAGTCC
    CTCATCGACG AAGGTGCCGA GGTGATCTAC AACGAGCTGA TGAGTGACTT TGAGATGGAT
    GAGAAGGACT TTACAGCCGA TTCCTGGAGC CTTGCAGTGG ATAGCAGCTT CTTGCAGCAG
    CATAAAAAGG AGGTGATGAA GCAGCAGGAT GTCATCTATG AGCTGATCCA GACAGAGCTA
    CACCACGTGC GGACGCTGAA GATCATGACC CGCCTCTTCC GCACGGGCAT GCTGGAAGAG
    CTGCAGCTGG AGCCGGGAGT GGTCCAGGGC CTGTTCCCCT GTGTGGATGA GCTCACTGAC
    ATCCACACGC GCTTCCTCAG CCAGCTGCTG GATCGCCGGC GCCAGGCCCT GTGCCCTGGC
    AGCACCCGGA ACTTCGTCAT CCATCGTTTG GGGGACCTGC TCATCAGCCA GTTCTCAGGT
    CCTAGCGCAG AGCAGATGCG GAAGACCTAC TCAGAGTTCT GTAGCCGCCA CACCAAGGCC
    TTAAAACTCT ATAAGGAGCT GTATGCCCGA GACAAACGCT TCCAGCAGTT CATCCGGAAA
    GTGACCCGCT CGGCGGTGCT GAAGCGGCAT GGGGTACAAG AGTGCATTCT GCTAGTGACT
    CAGCGCATCA CCAAGTACCC GGTGCTCATC AACCGCATCC TGCAGCATTC CCACGGGATG
    GAGGAGGAGC GCCAGGACCT GACCAAGGCC CTGGGGCTGG TGAAGGAGCT GCTGTCCAAC
    GTGGACCAGG ATGTGCACGA GCTGGAGAAA GGGGCCCGCC TGCAGGAGAT CTACAACCGC
    ATGGACCCTC GGGCCCAGGC CCCGGTTCCT GGCAAGGGCC CCTTTGGCCG AGAGGAGCTC
    CTGCGGCGCA AGCTCATCCA CGATGGTTGC CTGCTCTGGA AGACGGCGAC TGGGCGCTTC
    AAAGATGTGC TCATGCTGCT GATGACAGAT GTGCTGGTGT TTCTCCAAGA GAAGGACCAG
    AAGTACATCT TTCCTGCCCT GGACAAGCCC TCGGTGGTGT CACTGCAGAA TCTCATCGTG
    CGGGACATCG CTAACCAGGA GAAAGGGATG TTTCTGATCA GTGCGGCACC CCCTGAGATG
    TACGAGGTCC ACACGGCATC CCGGGATGAC CGGAGCACCT GGATCCGCGT CATTCAGCAG
    AGTGTGCGAG TATGCCCATC CAGGGAGGAC TTCCCCCTGA TTGAGACAGA GGATGAGGCT
    TACCTCCGGC GTATCAAGAT GGAGCTGCAG CAGAAAGACC GGGCACTGGT GGAGCTGCTG
    CAGGAGAAGG TTGGGCTATT TGCCGAGATG ACCCACTTCC AGGTGGAAGA GGATGGCAGC
    AGCGGGGTGC CCCTGCCCAC CCTGCCCAGG GGCCTCTTCC GCTCTGAGTC CCTTGAGTCC
    CCTCGTGGCG AGCGGCTGCT GCAGGATGCC ATCCGTGAGG TGGAGGGCCT GAAAGACCTG
    CTGGTAGGGC CTGGAGTGGA GCTGCTCTTG ACACCCCGGG AACCACCCTT GCCCATGGAG
    CCAGACAGCG GTGGTAACAC GAGTCCTGGA GTCACTGCCA ACGGTGAGGC CAGAACCTTC
    AATGGATCCA TTGAGCTCTG CAGAGCCGAC TCAGACTCCA GCCAGAAGGA TCGAAATGGA
    AATCAGCTGA GATCTCCCCA GGAGGAAGCG TTGCAGCGAT TGGTCAATCT CTATGGACTT
    CTGCATGGCC TACAGGCAGC TGTGGCCCAG CAGGACACTC TGATGGAAGC CCGGTTCCCT
    GAGGGCCCCG AGCGGCGGGA GAAGCTGACC AGAGCCAATT CCCGGGATGG GGAGGCTGGC
    AGAGCCGGGG GTGTCCCTGC GGTTCCTGAA AAGCAGGCTA CGGAACTGGC GTTGCTGCAG
    CGGCAACACG CACTATTGCA GGAGGAGCTG CGACGCTGCC GACGGCTCGG CGAGGAGCGT
    GCGACTGAGG CCGGCAGCTT GGAAGCCCGG CTCCGGGAGA GCGAGCAGGC CCGTGCCCTG
    CTGGAGCGGG AGGCCGAGGA GGCTCGCAGG CAGCTGGCCG CCTTGGGCCA CACGGAACCC
    CCACTCGTGG CTGAGGCCCC CTGGGCCCGC CGGCCTCTGG ATCCGCGGCG TCGGAGCCTC
    CCTGCTGGCG ATGCCCTGTA CTTGAGTTTC ACACCCCCAC AGCCCAGCCG AGGCCACGAC
    CGCCTGGATT TGCCTGTAAC TATTCGCTCT GTCCATCGAC CCTTTGAAGA CCGAGAGAGG
    CAGGAGCTGG GCAGCCCCGA CGAGCGGCTG CAAGATAGCA GTGACCCTGA CACCGGCAGC
    GAGGAGGAGG GTAGCAGCCG TCTGTCTCCA CCTCATAGTC CACGAGATTT CACCCGAATG
    CAGGACATCC CGGAAGAGAC TGAGAGCCGA GATGGGGAGC CTGTAGCTTC TGAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88160
    Clone ID Related Accession (Same CDS sequence) XM_015462085.1 , XM_015462085.2
    Accession Version XM_015462085.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTGCTGCT GCTGTGGCTG CTGCCCGCTG CTGGCCCACC TAGACAGGGG GTCCCTTGGA 
    GAGAGGACCC GGGAAAAGGA GAAGATGAAG GAAGCCAAGG ACGCACGCTA CACCAATGGG
    CACCTCTTCA CCACCATCTC CGTTTCGGGC ATGACCATGT GCTATGCCTG TAACAAGAGC
    ATCACAGCCA AGGAAGCCCT CATCTGCCCA ACCTGCAATG TGACTATCCA CAACCGCTGT
    AAAGACACCC TCGCCAACTG TACCAAGGTC AAGCAGAAGC AACAGAAAGC TGCCCTGCTG
    AAGAACAACA CTGCCTTGCA GTCCGTTTCC CTTCGCAGTA AGACTACCAC TCGGGAGCGG
    CCCAGCTCAG CCATCTACCC CTCCGACATC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC
    CGCTCCTCCT TGTCTTTAGC CAAAAGTGTC TCCACCACCA ACATTGCTGG ACACTTCAAT
    GATGAGTCTC CCCTGGGGCT TCGCCGGATC CTCTCACAGT CCACGGACTC CCTCAACATG
    CGGAACCGGA CACTGTCGGT GGAGTCCCTC ATCGACGAAG GTGCCGAGGT GATCTACAAC
    GAGCTGATGA GTGACTTTGA GATGGATGAG AAGGACTTTA CAGCCGATTC CTGGAGCCTT
    GCAGTGGATA GCAGCTTCTT GCAGCAGCAT AAAAAGGAGG TGATGAAGCA GCAGGATGTC
    ATCTATGAGC TGATCCAGAC AGAGCTACAC CACGTGCGGA CGCTGAAGAT CATGACCCGC
    CTCTTCCGCA CGGGCATGCT GGAAGAGCTG CAGCTGGAGC CGGGAGTGGT CCAGGGCCTG
    TTCCCCTGTG TGGATGAGCT CACTGACATC CACACGCGCT TCCTCAGCCA GCTGCTGGAT
    CGCCGGCGCC AGGCCCTGTG CCCTGGCAGC ACCCGGAACT TCGTCATCCA TCGTTTGGGG
    GACCTGCTCA TCAGCCAGTT CTCAGGTCCT AGCGCAGAGC AGATGCGGAA GACCTACTCA
    GAGTTCTGTA GCCGCCACAC CAAGGCCTTA AAACTCTATA AGGAGCTGTA TGCCCGAGAC
    AAACGCTTCC AGCAGTTCAT CCGGAAAGTG ACCCGCTCGG CGGTGCTGAA GCGGCATGGG
    GTACAAGAGT GCATTCTGCT AGTGACTCAG CGCATCACCA AGTACCCGGT GCTCATCAAC
    CGCATCCTGC AGCATTCCCA CGGGATGGAG GAGGAGCGCC AGGACCTGAC CAAGGCCCTG
    GGGCTGGTGA AGGAGCTGCT GTCCAACGTG GACCAGGATG TGCACGAGCT GGAGAAAGGG
    GCCCGCCTGC AGGAGATCTA CAACCGCATG GACCCTCGGG CCCAGGCCCC GGTTCCTGGC
    AAGGGCCCCT TTGGCCGAGA GGAGCTCCTG CGGCGCAAGC TCATCCACGA TGGTTGCCTG
    CTCTGGAAGA CGGCGACTGG GCGCTTCAAA GATGTGCTCA TGCTGCTGAT GACAGATGTG
    CTGGTGTTTC TCCAAGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCG
    GTGGTGTCAC TGCAGAATCT CATCGTGCGG GACATCGCTA ACCAGGAGAA AGGGATGTTT
    CTGATCAGTG CGGCACCCCC TGAGATGTAC GAGGTCCACA CGGCATCCCG GGATGACCGG
    AGCACCTGGA TCCGCGTCAT TCAGCAGAGT GTGCGAGTAT GCCCATCCAG GGAGGACTTC
    CCCCTGATTG AGACAGAGGA TGAGGCTTAC CTCCGGCGTA TCAAGATGGA GCTGCAGCAG
    AAAGACCGGG CACTGGTGGA GCTGCTGCAG GAGAAGGTTG GGCTATTTGC CGAGATGACC
    CACTTCCAGG TGGAAGAGGA TGGCAGCAGC GGGGTGCCCC TGCCCACCCT GCCCAGGGGC
    CTCTTCCGCT CTGAGTCCCT TGAGTCCCCT CGTGGCGAGC GGCTGCTGCA GGATGCCATC
    CGTGAGGTGG AGGGCCTGAA AGACCTGCTG GTAGGGCCTG GAGTGGAGCT GCTCTTGACA
    CCCCGGGAAC CACCCTTGCC CATGGAGCCA GACAGCGGTG GTAACACGAG TCCTGGAGTC
    ACTGCCAACG GTGAGGCCAG AACCTTCAAT GGATCCATTG AGCTCTGCAG AGCCGACTCA
    GACTCCAGCC AGAAGGATCG AAATGGAAAT CAGCTGAGAT CTCCCCAGGA GGAAGCGTTG
    CAGCGATTGG TCAATCTCTA TGGACTTCTG CATGGCCTAC AGGCAGCTGT GGCCCAGCAG
    GACACTCTGA TGGAAGCCCG GTTCCCTGAG GGCCCCGAGC GGCGGGAGAA GCTGACCAGA
    GCCAATTCCC GGGATGGGGA GGCTGGCAGA GCCGGGGGTG TCCCTGCGGT TCCTGAAAAG
    CAGGCTACGG AACTGGCGTT GCTGCAGCGG CAACACGCAC TATTGCAGGA GGAGCTGCGA
    CGCTGCCGAC GGCTCGGCGA GGAGCGTGCG ACTGAGGCCG GCAGCTTGGA AGCCCGGCTC
    CGGGAGAGCG AGCAGGCCCG TGCCCTGCTG GAGCGGGAGG CCGAGGAGGC TCGCAGGCAG
    CTGGCCGCCT TGGGCCACAC GGAACCCCCA CTCGTGGCTG AGGCCCCCTG GGCCCGCCGG
    CCTCTGGATC CGCGGCGTCG GAGCCTCCCT GCTGGCGATG CCCTGTACTT GAGTTTCACA
    CCCCCACAGC CCAGCCGAGG CCACGACCGC CTGGATTTGC CTGTAACTAT TCGCTCTGTC
    CATCGACCCT TTGAAGACCG AGAGAGGCAG GAGCTGGGCA GCCCCGACGA GCGGCTGCAA
    GATAGCAGTG ACCCTGACAC CGGCAGCGAG GAGGAGGGTA GCAGCCGTCT GTCTCCACCT
    CATAGTCCAC GAGATTTCAC CCGAATGCAG GACATCCCGG AAGAGACTGA GAGCCGAGAT
    GGGGAGCCTG TAGCTTCTGA GAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317571.1
    CDS1842..4808
    Translation

    Target ORF information:

    RefSeq Version XM_015462085.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTGCTGCT GCTGTGGCTG CTGCCCGCTG CTGGCCCACC TAGACAGGGG GTCCCTTGGA 
    GAGAGGACCC GGGAAAAGGA GAAGATGAAG GAAGCCAAGG ACGCACGCTA CACCAATGGG
    CACCTCTTCA CCACCATCTC CGTTTCGGGC ATGACCATGT GCTATGCCTG TAACAAGAGC
    ATCACAGCCA AGGAAGCCCT CATCTGCCCA ACCTGCAATG TGACTATCCA CAACCGCTGT
    AAAGACACCC TCGCCAACTG TACCAAGGTC AAGCAGAAGC AACAGAAAGC TGCCCTGCTG
    AAGAACAACA CTGCCTTGCA GTCCGTTTCC CTTCGCAGTA AGACTACCAC TCGGGAGCGG
    CCCAGCTCAG CCATCTACCC CTCCGACATC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC
    CGCTCCTCCT TGTCTTTAGC CAAAAGTGTC TCCACCACCA ACATTGCTGG ACACTTCAAT
    GATGAGTCTC CCCTGGGGCT TCGCCGGATC CTCTCACAGT CCACGGACTC CCTCAACATG
    CGGAACCGGA CACTGTCGGT GGAGTCCCTC ATCGACGAAG GTGCCGAGGT GATCTACAAC
    GAGCTGATGA GTGACTTTGA GATGGATGAG AAGGACTTTA CAGCCGATTC CTGGAGCCTT
    GCAGTGGATA GCAGCTTCTT GCAGCAGCAT AAAAAGGAGG TGATGAAGCA GCAGGATGTC
    ATCTATGAGC TGATCCAGAC AGAGCTACAC CACGTGCGGA CGCTGAAGAT CATGACCCGC
    CTCTTCCGCA CGGGCATGCT GGAAGAGCTG CAGCTGGAGC CGGGAGTGGT CCAGGGCCTG
    TTCCCCTGTG TGGATGAGCT CACTGACATC CACACGCGCT TCCTCAGCCA GCTGCTGGAT
    CGCCGGCGCC AGGCCCTGTG CCCTGGCAGC ACCCGGAACT TCGTCATCCA TCGTTTGGGG
    GACCTGCTCA TCAGCCAGTT CTCAGGTCCT AGCGCAGAGC AGATGCGGAA GACCTACTCA
    GAGTTCTGTA GCCGCCACAC CAAGGCCTTA AAACTCTATA AGGAGCTGTA TGCCCGAGAC
    AAACGCTTCC AGCAGTTCAT CCGGAAAGTG ACCCGCTCGG CGGTGCTGAA GCGGCATGGG
    GTACAAGAGT GCATTCTGCT AGTGACTCAG CGCATCACCA AGTACCCGGT GCTCATCAAC
    CGCATCCTGC AGCATTCCCA CGGGATGGAG GAGGAGCGCC AGGACCTGAC CAAGGCCCTG
    GGGCTGGTGA AGGAGCTGCT GTCCAACGTG GACCAGGATG TGCACGAGCT GGAGAAAGGG
    GCCCGCCTGC AGGAGATCTA CAACCGCATG GACCCTCGGG CCCAGGCCCC GGTTCCTGGC
    AAGGGCCCCT TTGGCCGAGA GGAGCTCCTG CGGCGCAAGC TCATCCACGA TGGTTGCCTG
    CTCTGGAAGA CGGCGACTGG GCGCTTCAAA GATGTGCTCA TGCTGCTGAT GACAGATGTG
    CTGGTGTTTC TCCAAGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCG
    GTGGTGTCAC TGCAGAATCT CATCGTGCGG GACATCGCTA ACCAGGAGAA AGGGATGTTT
    CTGATCAGTG CGGCACCCCC TGAGATGTAC GAGGTCCACA CGGCATCCCG GGATGACCGG
    AGCACCTGGA TCCGCGTCAT TCAGCAGAGT GTGCGAGTAT GCCCATCCAG GGAGGACTTC
    CCCCTGATTG AGACAGAGGA TGAGGCTTAC CTCCGGCGTA TCAAGATGGA GCTGCAGCAG
    AAAGACCGGG CACTGGTGGA GCTGCTGCAG GAGAAGGTTG GGCTATTTGC CGAGATGACC
    CACTTCCAGG TGGAAGAGGA TGGCAGCAGC GGGGTGCCCC TGCCCACCCT GCCCAGGGGC
    CTCTTCCGCT CTGAGTCCCT TGAGTCCCCT CGTGGCGAGC GGCTGCTGCA GGATGCCATC
    CGTGAGGTGG AGGGCCTGAA AGACCTGCTG GTAGGGCCTG GAGTGGAGCT GCTCTTGACA
    CCCCGGGAAC CACCCTTGCC CATGGAGCCA GACAGCGGTG GTAACACGAG TCCTGGAGTC
    ACTGCCAACG GTGAGGCCAG AACCTTCAAT GGATCCATTG AGCTCTGCAG AGCCGACTCA
    GACTCCAGCC AGAAGGATCG AAATGGAAAT CAGCTGAGAT CTCCCCAGGA GGAAGCGTTG
    CAGCGATTGG TCAATCTCTA TGGACTTCTG CATGGCCTAC AGGCAGCTGT GGCCCAGCAG
    GACACTCTGA TGGAAGCCCG GTTCCCTGAG GGCCCCGAGC GGCGGGAGAA GCTGACCAGA
    GCCAATTCCC GGGATGGGGA GGCTGGCAGA GCCGGGGGTG TCCCTGCGGT TCCTGAAAAG
    CAGGCTACGG AACTGGCGTT GCTGCAGCGG CAACACGCAC TATTGCAGGA GGAGCTGCGA
    CGCTGCCGAC GGCTCGGCGA GGAGCGTGCG ACTGAGGCCG GCAGCTTGGA AGCCCGGCTC
    CGGGAGAGCG AGCAGGCCCG TGCCCTGCTG GAGCGGGAGG CCGAGGAGGC TCGCAGGCAG
    CTGGCCGCCT TGGGCCACAC GGAACCCCCA CTCGTGGCTG AGGCCCCCTG GGCCCGCCGG
    CCTCTGGATC CGCGGCGTCG GAGCCTCCCT GCTGGCGATG CCCTGTACTT GAGTTTCACA
    CCCCCACAGC CCAGCCGAGG CCACGACCGC CTGGATTTGC CTGTAACTAT TCGCTCTGTC
    CATCGACCCT TTGAAGACCG AGAGAGGCAG GAGCTGGGCA GCCCCGACGA GCGGCTGCAA
    GATAGCAGTG ACCCTGACAC CGGCAGCGAG GAGGAGGGTA GCAGCCGTCT GTCTCCACCT
    CATAGTCCAC GAGATTTCAC CCGAATGCAG GACATCCCGG AAGAGACTGA GAGCCGAGAT
    GGGGAGCCTG TAGCTTCTGA GAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88160
    Clone ID Related Accession (Same CDS sequence) XM_015462085.1 , XM_015462085.2
    Accession Version XM_015462085.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2967bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015462085.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTGCTGCT GCTGTGGCTG CTGCCCGCTG CTGGCCCACC TAGACAGGGG GTCCCTTGGA 
    GAGAGGACCC GGGAAAAGGA GAAGATGAAG GAAGCCAAGG ACGCACGCTA CACCAATGGG
    CACCTCTTCA CCACCATCTC CGTTTCGGGC ATGACCATGT GCTATGCCTG TAACAAGAGC
    ATCACAGCCA AGGAAGCCCT CATCTGCCCA ACCTGCAATG TGACTATCCA CAACCGCTGT
    AAAGACACCC TCGCCAACTG TACCAAGGTC AAGCAGAAGC AACAGAAAGC TGCCCTGCTG
    AAGAACAACA CTGCCTTGCA GTCCGTTTCC CTTCGCAGTA AGACTACCAC TCGGGAGCGG
    CCCAGCTCAG CCATCTACCC CTCCGACATC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC
    CGCTCCTCCT TGTCTTTAGC CAAAAGTGTC TCCACCACCA ACATTGCTGG ACACTTCAAT
    GATGAGTCTC CCCTGGGGCT TCGCCGGATC CTCTCACAGT CCACGGACTC CCTCAACATG
    CGGAACCGGA CACTGTCGGT GGAGTCCCTC ATCGACGAAG GTGCCGAGGT GATCTACAAC
    GAGCTGATGA GTGACTTTGA GATGGATGAG AAGGACTTTA CAGCCGATTC CTGGAGCCTT
    GCAGTGGATA GCAGCTTCTT GCAGCAGCAT AAAAAGGAGG TGATGAAGCA GCAGGATGTC
    ATCTATGAGC TGATCCAGAC AGAGCTACAC CACGTGCGGA CGCTGAAGAT CATGACCCGC
    CTCTTCCGCA CGGGCATGCT GGAAGAGCTG CAGCTGGAGC CGGGAGTGGT CCAGGGCCTG
    TTCCCCTGTG TGGATGAGCT CACTGACATC CACACGCGCT TCCTCAGCCA GCTGCTGGAT
    CGCCGGCGCC AGGCCCTGTG CCCTGGCAGC ACCCGGAACT TCGTCATCCA TCGTTTGGGG
    GACCTGCTCA TCAGCCAGTT CTCAGGTCCT AGCGCAGAGC AGATGCGGAA GACCTACTCA
    GAGTTCTGTA GCCGCCACAC CAAGGCCTTA AAACTCTATA AGGAGCTGTA TGCCCGAGAC
    AAACGCTTCC AGCAGTTCAT CCGGAAAGTG ACCCGCTCGG CGGTGCTGAA GCGGCATGGG
    GTACAAGAGT GCATTCTGCT AGTGACTCAG CGCATCACCA AGTACCCGGT GCTCATCAAC
    CGCATCCTGC AGCATTCCCA CGGGATGGAG GAGGAGCGCC AGGACCTGAC CAAGGCCCTG
    GGGCTGGTGA AGGAGCTGCT GTCCAACGTG GACCAGGATG TGCACGAGCT GGAGAAAGGG
    GCCCGCCTGC AGGAGATCTA CAACCGCATG GACCCTCGGG CCCAGGCCCC GGTTCCTGGC
    AAGGGCCCCT TTGGCCGAGA GGAGCTCCTG CGGCGCAAGC TCATCCACGA TGGTTGCCTG
    CTCTGGAAGA CGGCGACTGG GCGCTTCAAA GATGTGCTCA TGCTGCTGAT GACAGATGTG
    CTGGTGTTTC TCCAAGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCG
    GTGGTGTCAC TGCAGAATCT CATCGTGCGG GACATCGCTA ACCAGGAGAA AGGGATGTTT
    CTGATCAGTG CGGCACCCCC TGAGATGTAC GAGGTCCACA CGGCATCCCG GGATGACCGG
    AGCACCTGGA TCCGCGTCAT TCAGCAGAGT GTGCGAGTAT GCCCATCCAG GGAGGACTTC
    CCCCTGATTG AGACAGAGGA TGAGGCTTAC CTCCGGCGTA TCAAGATGGA GCTGCAGCAG
    AAAGACCGGG CACTGGTGGA GCTGCTGCAG GAGAAGGTTG GGCTATTTGC CGAGATGACC
    CACTTCCAGG TGGAAGAGGA TGGCAGCAGC GGGGTGCCCC TGCCCACCCT GCCCAGGGGC
    CTCTTCCGCT CTGAGTCCCT TGAGTCCCCT CGTGGCGAGC GGCTGCTGCA GGATGCCATC
    CGTGAGGTGG AGGGCCTGAA AGACCTGCTG GTAGGGCCTG GAGTGGAGCT GCTCTTGACA
    CCCCGGGAAC CACCCTTGCC CATGGAGCCA GACAGCGGTG GTAACACGAG TCCTGGAGTC
    ACTGCCAACG GTGAGGCCAG AACCTTCAAT GGATCCATTG AGCTCTGCAG AGCCGACTCA
    GACTCCAGCC AGAAGGATCG AAATGGAAAT CAGCTGAGAT CTCCCCAGGA GGAAGCGTTG
    CAGCGATTGG TCAATCTCTA TGGACTTCTG CATGGCCTAC AGGCAGCTGT GGCCCAGCAG
    GACACTCTGA TGGAAGCCCG GTTCCCTGAG GGCCCCGAGC GGCGGGAGAA GCTGACCAGA
    GCCAATTCCC GGGATGGGGA GGCTGGCAGA GCCGGGGGTG TCCCTGCGGT TCCTGAAAAG
    CAGGCTACGG AACTGGCGTT GCTGCAGCGG CAACACGCAC TATTGCAGGA GGAGCTGCGA
    CGCTGCCGAC GGCTCGGCGA GGAGCGTGCG ACTGAGGCCG GCAGCTTGGA AGCCCGGCTC
    CGGGAGAGCG AGCAGGCCCG TGCCCTGCTG GAGCGGGAGG CCGAGGAGGC TCGCAGGCAG
    CTGGCCGCCT TGGGCCACAC GGAACCCCCA CTCGTGGCTG AGGCCCCCTG GGCCCGCCGG
    CCTCTGGATC CGCGGCGTCG GAGCCTCCCT GCTGGCGATG CCCTGTACTT GAGTTTCACA
    CCCCCACAGC CCAGCCGAGG CCACGACCGC CTGGATTTGC CTGTAACTAT TCGCTCTGTC
    CATCGACCCT TTGAAGACCG AGAGAGGCAG GAGCTGGGCA GCCCCGACGA GCGGCTGCAA
    GATAGCAGTG ACCCTGACAC CGGCAGCGAG GAGGAGGGTA GCAGCCGTCT GTCTCCACCT
    CATAGTCCAC GAGATTTCAC CCGAATGCAG GACATCCCGG AAGAGACTGA GAGCCGAGAT
    GGGGAGCCTG TAGCTTCTGA GAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317571.1
    CDS21..2987
    Translation

    Target ORF information:

    RefSeq Version XM_015462085.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462085.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTGCTGCT GCTGTGGCTG CTGCCCGCTG CTGGCCCACC TAGACAGGGG GTCCCTTGGA 
    GAGAGGACCC GGGAAAAGGA GAAGATGAAG GAAGCCAAGG ACGCACGCTA CACCAATGGG
    CACCTCTTCA CCACCATCTC CGTTTCGGGC ATGACCATGT GCTATGCCTG TAACAAGAGC
    ATCACAGCCA AGGAAGCCCT CATCTGCCCA ACCTGCAATG TGACTATCCA CAACCGCTGT
    AAAGACACCC TCGCCAACTG TACCAAGGTC AAGCAGAAGC AACAGAAAGC TGCCCTGCTG
    AAGAACAACA CTGCCTTGCA GTCCGTTTCC CTTCGCAGTA AGACTACCAC TCGGGAGCGG
    CCCAGCTCAG CCATCTACCC CTCCGACATC CGGCAGTCCC TGCTCGGCTC CCGCCGTGGC
    CGCTCCTCCT TGTCTTTAGC CAAAAGTGTC TCCACCACCA ACATTGCTGG ACACTTCAAT
    GATGAGTCTC CCCTGGGGCT TCGCCGGATC CTCTCACAGT CCACGGACTC CCTCAACATG
    CGGAACCGGA CACTGTCGGT GGAGTCCCTC ATCGACGAAG GTGCCGAGGT GATCTACAAC
    GAGCTGATGA GTGACTTTGA GATGGATGAG AAGGACTTTA CAGCCGATTC CTGGAGCCTT
    GCAGTGGATA GCAGCTTCTT GCAGCAGCAT AAAAAGGAGG TGATGAAGCA GCAGGATGTC
    ATCTATGAGC TGATCCAGAC AGAGCTACAC CACGTGCGGA CGCTGAAGAT CATGACCCGC
    CTCTTCCGCA CGGGCATGCT GGAAGAGCTG CAGCTGGAGC CGGGAGTGGT CCAGGGCCTG
    TTCCCCTGTG TGGATGAGCT CACTGACATC CACACGCGCT TCCTCAGCCA GCTGCTGGAT
    CGCCGGCGCC AGGCCCTGTG CCCTGGCAGC ACCCGGAACT TCGTCATCCA TCGTTTGGGG
    GACCTGCTCA TCAGCCAGTT CTCAGGTCCT AGCGCAGAGC AGATGCGGAA GACCTACTCA
    GAGTTCTGTA GCCGCCACAC CAAGGCCTTA AAACTCTATA AGGAGCTGTA TGCCCGAGAC
    AAACGCTTCC AGCAGTTCAT CCGGAAAGTG ACCCGCTCGG CGGTGCTGAA GCGGCATGGG
    GTACAAGAGT GCATTCTGCT AGTGACTCAG CGCATCACCA AGTACCCGGT GCTCATCAAC
    CGCATCCTGC AGCATTCCCA CGGGATGGAG GAGGAGCGCC AGGACCTGAC CAAGGCCCTG
    GGGCTGGTGA AGGAGCTGCT GTCCAACGTG GACCAGGATG TGCACGAGCT GGAGAAAGGG
    GCCCGCCTGC AGGAGATCTA CAACCGCATG GACCCTCGGG CCCAGGCCCC GGTTCCTGGC
    AAGGGCCCCT TTGGCCGAGA GGAGCTCCTG CGGCGCAAGC TCATCCACGA TGGTTGCCTG
    CTCTGGAAGA CGGCGACTGG GCGCTTCAAA GATGTGCTCA TGCTGCTGAT GACAGATGTG
    CTGGTGTTTC TCCAAGAGAA GGACCAGAAG TACATCTTTC CTGCCCTGGA CAAGCCCTCG
    GTGGTGTCAC TGCAGAATCT CATCGTGCGG GACATCGCTA ACCAGGAGAA AGGGATGTTT
    CTGATCAGTG CGGCACCCCC TGAGATGTAC GAGGTCCACA CGGCATCCCG GGATGACCGG
    AGCACCTGGA TCCGCGTCAT TCAGCAGAGT GTGCGAGTAT GCCCATCCAG GGAGGACTTC
    CCCCTGATTG AGACAGAGGA TGAGGCTTAC CTCCGGCGTA TCAAGATGGA GCTGCAGCAG
    AAAGACCGGG CACTGGTGGA GCTGCTGCAG GAGAAGGTTG GGCTATTTGC CGAGATGACC
    CACTTCCAGG TGGAAGAGGA TGGCAGCAGC GGGGTGCCCC TGCCCACCCT GCCCAGGGGC
    CTCTTCCGCT CTGAGTCCCT TGAGTCCCCT CGTGGCGAGC GGCTGCTGCA GGATGCCATC
    CGTGAGGTGG AGGGCCTGAA AGACCTGCTG GTAGGGCCTG GAGTGGAGCT GCTCTTGACA
    CCCCGGGAAC CACCCTTGCC CATGGAGCCA GACAGCGGTG GTAACACGAG TCCTGGAGTC
    ACTGCCAACG GTGAGGCCAG AACCTTCAAT GGATCCATTG AGCTCTGCAG AGCCGACTCA
    GACTCCAGCC AGAAGGATCG AAATGGAAAT CAGCTGAGAT CTCCCCAGGA GGAAGCGTTG
    CAGCGATTGG TCAATCTCTA TGGACTTCTG CATGGCCTAC AGGCAGCTGT GGCCCAGCAG
    GACACTCTGA TGGAAGCCCG GTTCCCTGAG GGCCCCGAGC GGCGGGAGAA GCTGACCAGA
    GCCAATTCCC GGGATGGGGA GGCTGGCAGA GCCGGGGGTG TCCCTGCGGT TCCTGAAAAG
    CAGGCTACGG AACTGGCGTT GCTGCAGCGG CAACACGCAC TATTGCAGGA GGAGCTGCGA
    CGCTGCCGAC GGCTCGGCGA GGAGCGTGCG ACTGAGGCCG GCAGCTTGGA AGCCCGGCTC
    CGGGAGAGCG AGCAGGCCCG TGCCCTGCTG GAGCGGGAGG CCGAGGAGGC TCGCAGGCAG
    CTGGCCGCCT TGGGCCACAC GGAACCCCCA CTCGTGGCTG AGGCCCCCTG GGCCCGCCGG
    CCTCTGGATC CGCGGCGTCG GAGCCTCCCT GCTGGCGATG CCCTGTACTT GAGTTTCACA
    CCCCCACAGC CCAGCCGAGG CCACGACCGC CTGGATTTGC CTGTAACTAT TCGCTCTGTC
    CATCGACCCT TTGAAGACCG AGAGAGGCAG GAGCTGGGCA GCCCCGACGA GCGGCTGCAA
    GATAGCAGTG ACCCTGACAC CGGCAGCGAG GAGGAGGGTA GCAGCCGTCT GTCTCCACCT
    CATAGTCCAC GAGATTTCAC CCGAATGCAG GACATCCCGG AAGAGACTGA GAGCCGAGAT
    GGGGAGCCTG TAGCTTCTGA GAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa17273
    Clone ID Related Accession (Same CDS sequence) XM_024986243.1
    Accession Version XM_024986243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3546bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGATCAAG GTTCCTGTCA CCCACCAGCT
    GGCCCTTTGG ACCACTCAGC CACTGGTATG CTTTCCAAGT CTGTATCCAT GAGTGGCATC
    AATTGCTTCT TGGGCTGCTC AGACCCAGCT GGGAATTTGT CCCAGTCTTC TGGCACTGAC
    CTGAGCCAAA GCTGTAGCCA ACTGGAACGG AGCTCAGGTA CCTGGGCAGG CTCCTCCCTG
    CGACGGACAC TCAGCTTGAT CTTGGGCATG ACCGGAAAGG CCAAGACCCG GGAAAAGGAG
    AAGATGAAGG AAGCCAAGGA CGCACGCTAC ACCAATGGGC ACCTCTTCAC CACCATCTCC
    GTTTCGGGCA TGACCATGTG CTATGCCTGT AACAAGAGCA TCACAGCCAA GGAAGCCCTC
    ATCTGCCCAA CCTGCAATGT GACTATCCAC AACCGCTGTA AAGACACCCT CGCCAACTGT
    ACCAAGGTCA AGCAGAAGCA ACAGAAAGCT GCCCTGCTGA AGAACAACAC TGCCTTGCAG
    TCCGTTTCCC TTCGCAGTAA GACTACCACT CGGGAGCGGC CCAGCTCAGC CATCTACCCC
    TCCGACATCC GGCAGTCCCT GCTCGGCTCC CGCCGTGGCC GCTCCTCCTT GTCTTTAGCC
    AAAAGTGTCT CCACCACCAA CATTGCTGGA CACTTCAATG ATGAGTCTCC CCTGGGGCTT
    CGCCGGATCC TCTCACAGTC CACGGACTCC CTCAACATGC GGAACCGGAC ACTGTCGGTG
    GAGTCCCTCA TCGACGAAGG TGCCGAGGTG ATCTACAACG AGCTGATGAG TGACTTTGAG
    ATGGATGAGA AGGACTTTAC AGCCGATTCC TGGAGCCTTG CAGTGGATAG CAGCTTCTTG
    CAGCAGCATA AAAAGGAGGT GATGAAGCAG CAGGATGTCA TCTATGAGCT GATCCAGACA
    GAGCTACACC ACGTGCGGAC GCTGAAGATC ATGACCCGCC TCTTCCGCAC GGGCATGCTG
    GAAGAGCTGC AGCTGGAGCC GGGAGTGGTC CAGGGCCTGT TCCCCTGTGT GGATGAGCTC
    ACTGACATCC ACACGCGCTT CCTCAGCCAG CTGCTGGATC GCCGGCGCCA GGCCCTGTGC
    CCTGGCAGCA CCCGGAACTT CGTCATCCAT CGTTTGGGGG ACCTGCTCAT CAGCCAGTTC
    TCAGGTCCTA GCGCAGAGCA GATGCGGAAG ACCTACTCAG AGTTCTGTAG CCGCCACACC
    AAGGCCTTAA AACTCTATAA GGAGCTGTAT GCCCGAGACA AACGCTTCCA GCAGTTCATC
    CGGAAAGTGA CCCGCTCGGC GGTGCTGAAG CGGCATGGGG TACAAGAGTG CATTCTGCTA
    GTGACTCAGC GCATCACCAA GTACCCGGTG CTCATCAACC GCATCCTGCA GCATTCCCAC
    GGGATGGAGG AGGAGCGCCA GGACCTGACC AAGGCCCTGG GGCTGGTGAA GGAGCTGCTG
    TCCAACGTGG ACCAGGATGT GCACGAGCTG GAGAAAGGGG CCCGCCTGCA GGAGATCTAC
    AACCGCATGG ACCCTCGGGC CCAGGCCCCG GTTCCTGGCA AGGGCCCCTT TGGCCGAGAG
    GAGCTCCTGC GGCGCAAGCT CATCCACGAT GGTTGCCTGC TCTGGAAGAC GGCGACTGGG
    CGCTTCAAAG ATGTGCTCAT GCTGCTGATG ACAGATGTGC TGGTGTTTCT CCAAGAGAAG
    GACCAGAAGT ACATCTTTCC TGCCCTGGAC AAGCCCTCGG TGGTGTCACT GCAGAATCTC
    ATCGTGCGGG ACATCGCTAA CCAGGAGAAA GGGATGTTTC TGATCAGTGC GGCACCCCCT
    GAGATGTACG AGGTCCACAC GGCATCCCGG GATGACCGGA GCACCTGGAT CCGCGTCATT
    CAGCAGAGTG TGCGAGTATG CCCATCCAGG GAGGACTTCC CCCTGATTGA GACAGAGGAT
    GAGGCTTACC TCCGGCGTAT CAAGATGGAG CTGCAGCAGA AAGACCGGGC ACTGGTGGAG
    CTGCTGCAGG AGAAGGTTGG GCTATTTGCC GAGATGACCC ACTTCCAGGT GGAAGAGGAT
    GGCAGCAGCG GGGTGCCCCT GCCCACCCTG CCCAGGGGCC TCTTCCGCTC TGAGTCCCTT
    GAGTCCCCTC GTGGCGAGCG GCTGCTGCAG GATGCCATCC GTGAGGTGGA GGGCCTGAAA
    GACCTGCTGG TAGGGCCTGG AGTGGAGCTG CTCTTGACAC CCCGGGAACC ACCCTTGCCC
    ATGGAGCCAG ACAGCGGTGG TAACACGAGT CCTGGAGTCA CTGCCAACGG TGAGGCCAGA
    ACCTTCAATG GATCCATTGA GCTCTGCAGA GCCGACTCAG ACTCCAGCCA GAAGGATCGA
    AATGGAAATC AGCTGAGATC TCCCCAGGAG GAAGCGTTGC AGCGATTGGT CAATCTCTAT
    GGACTTCTGC ATGGCCTACA GGCAGCTGTG GCCCAGCAGG ACACTCTGAT GGAAGCCCGG
    TTCCCTGAGG GCCCCGAGCG GCGGGAGAAG CTGACCAGAG CCAATTCCCG GGATGGGGAG
    GCTGGCAGAG CCGGGGGTGT CCCTGCGGTT CCTGAAAAGC AGGCTACGGA ACTGGCGTTG
    CTGCAGCGGC AACACGCACT ATTGCAGGAG GAGCTGCGAC GCTGCCGACG GCTCGGCGAG
    GAGCGTGCGA CTGAGGCCGG CAGCTTGGAA GCCCGGCTCC GGGAGAGCGA GCAGGCCCGT
    GCCCTGCTGG AGCGGGAGGC CGAGGAGGCT CGCAGGCAGC TGGCCGCCTT GGGCCACACG
    GAACCCCCAC TCGTGGCTGA GGCCCCCTGG GCCCGCCGGC CTCTGGATCC GCGGCGTCGG
    AGCCTCCCTG CTGGCGATGC CCTGTACTTG AGTTTCACAC CCCCACAGCC CAGCCGAGGC
    CACGACCGCC TGGATTTGCC TGTAACTATT CGCTCTGTCC ATCGACCCTT TGAAGACCGA
    GAGAGGCAGG AGCTGGGCAG CCCCGACGAG CGGCTGCAAG ATAGCAGTGA CCCTGACACC
    GGCAGCGAGG AGGAGGGTAG CAGCCGTCTG TCTCCACCTC ATAGTCCACG AGATTTCACC
    CGAATGCAGG ACATCCCGGA AGAGACTGAG AGCCGAGATG GGGAGCCTGT AGCTTCTGAG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842011.1
    CDS16..3561
    Translation

    Target ORF information:

    RefSeq Version XM_024986243.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGATCAAG GTTCCTGTCA CCCACCAGCT
    GGCCCTTTGG ACCACTCAGC CACTGGTATG CTTTCCAAGT CTGTATCCAT GAGTGGCATC
    AATTGCTTCT TGGGCTGCTC AGACCCAGCT GGGAATTTGT CCCAGTCTTC TGGCACTGAC
    CTGAGCCAAA GCTGTAGCCA ACTGGAACGG AGCTCAGGTA CCTGGGCAGG CTCCTCCCTG
    CGACGGACAC TCAGCTTGAT CTTGGGCATG ACCGGAAAGG CCAAGACCCG GGAAAAGGAG
    AAGATGAAGG AAGCCAAGGA CGCACGCTAC ACCAATGGGC ACCTCTTCAC CACCATCTCC
    GTTTCGGGCA TGACCATGTG CTATGCCTGT AACAAGAGCA TCACAGCCAA GGAAGCCCTC
    ATCTGCCCAA CCTGCAATGT GACTATCCAC AACCGCTGTA AAGACACCCT CGCCAACTGT
    ACCAAGGTCA AGCAGAAGCA ACAGAAAGCT GCCCTGCTGA AGAACAACAC TGCCTTGCAG
    TCCGTTTCCC TTCGCAGTAA GACTACCACT CGGGAGCGGC CCAGCTCAGC CATCTACCCC
    TCCGACATCC GGCAGTCCCT GCTCGGCTCC CGCCGTGGCC GCTCCTCCTT GTCTTTAGCC
    AAAAGTGTCT CCACCACCAA CATTGCTGGA CACTTCAATG ATGAGTCTCC CCTGGGGCTT
    CGCCGGATCC TCTCACAGTC CACGGACTCC CTCAACATGC GGAACCGGAC ACTGTCGGTG
    GAGTCCCTCA TCGACGAAGG TGCCGAGGTG ATCTACAACG AGCTGATGAG TGACTTTGAG
    ATGGATGAGA AGGACTTTAC AGCCGATTCC TGGAGCCTTG CAGTGGATAG CAGCTTCTTG
    CAGCAGCATA AAAAGGAGGT GATGAAGCAG CAGGATGTCA TCTATGAGCT GATCCAGACA
    GAGCTACACC ACGTGCGGAC GCTGAAGATC ATGACCCGCC TCTTCCGCAC GGGCATGCTG
    GAAGAGCTGC AGCTGGAGCC GGGAGTGGTC CAGGGCCTGT TCCCCTGTGT GGATGAGCTC
    ACTGACATCC ACACGCGCTT CCTCAGCCAG CTGCTGGATC GCCGGCGCCA GGCCCTGTGC
    CCTGGCAGCA CCCGGAACTT CGTCATCCAT CGTTTGGGGG ACCTGCTCAT CAGCCAGTTC
    TCAGGTCCTA GCGCAGAGCA GATGCGGAAG ACCTACTCAG AGTTCTGTAG CCGCCACACC
    AAGGCCTTAA AACTCTATAA GGAGCTGTAT GCCCGAGACA AACGCTTCCA GCAGTTCATC
    CGGAAAGTGA CCCGCTCGGC GGTGCTGAAG CGGCATGGGG TACAAGAGTG CATTCTGCTA
    GTGACTCAGC GCATCACCAA GTACCCGGTG CTCATCAACC GCATCCTGCA GCATTCCCAC
    GGGATGGAGG AGGAGCGCCA GGACCTGACC AAGGCCCTGG GGCTGGTGAA GGAGCTGCTG
    TCCAACGTGG ACCAGGATGT GCACGAGCTG GAGAAAGGGG CCCGCCTGCA GGAGATCTAC
    AACCGCATGG ACCCTCGGGC CCAGGCCCCG GTTCCTGGCA AGGGCCCCTT TGGCCGAGAG
    GAGCTCCTGC GGCGCAAGCT CATCCACGAT GGTTGCCTGC TCTGGAAGAC GGCGACTGGG
    CGCTTCAAAG ATGTGCTCAT GCTGCTGATG ACAGATGTGC TGGTGTTTCT CCAAGAGAAG
    GACCAGAAGT ACATCTTTCC TGCCCTGGAC AAGCCCTCGG TGGTGTCACT GCAGAATCTC
    ATCGTGCGGG ACATCGCTAA CCAGGAGAAA GGGATGTTTC TGATCAGTGC GGCACCCCCT
    GAGATGTACG AGGTCCACAC GGCATCCCGG GATGACCGGA GCACCTGGAT CCGCGTCATT
    CAGCAGAGTG TGCGAGTATG CCCATCCAGG GAGGACTTCC CCCTGATTGA GACAGAGGAT
    GAGGCTTACC TCCGGCGTAT CAAGATGGAG CTGCAGCAGA AAGACCGGGC ACTGGTGGAG
    CTGCTGCAGG AGAAGGTTGG GCTATTTGCC GAGATGACCC ACTTCCAGGT GGAAGAGGAT
    GGCAGCAGCG GGGTGCCCCT GCCCACCCTG CCCAGGGGCC TCTTCCGCTC TGAGTCCCTT
    GAGTCCCCTC GTGGCGAGCG GCTGCTGCAG GATGCCATCC GTGAGGTGGA GGGCCTGAAA
    GACCTGCTGG TAGGGCCTGG AGTGGAGCTG CTCTTGACAC CCCGGGAACC ACCCTTGCCC
    ATGGAGCCAG ACAGCGGTGG TAACACGAGT CCTGGAGTCA CTGCCAACGG TGAGGCCAGA
    ACCTTCAATG GATCCATTGA GCTCTGCAGA GCCGACTCAG ACTCCAGCCA GAAGGATCGA
    AATGGAAATC AGCTGAGATC TCCCCAGGAG GAAGCGTTGC AGCGATTGGT CAATCTCTAT
    GGACTTCTGC ATGGCCTACA GGCAGCTGTG GCCCAGCAGG ACACTCTGAT GGAAGCCCGG
    TTCCCTGAGG GCCCCGAGCG GCGGGAGAAG CTGACCAGAG CCAATTCCCG GGATGGGGAG
    GCTGGCAGAG CCGGGGGTGT CCCTGCGGTT CCTGAAAAGC AGGCTACGGA ACTGGCGTTG
    CTGCAGCGGC AACACGCACT ATTGCAGGAG GAGCTGCGAC GCTGCCGACG GCTCGGCGAG
    GAGCGTGCGA CTGAGGCCGG CAGCTTGGAA GCCCGGCTCC GGGAGAGCGA GCAGGCCCGT
    GCCCTGCTGG AGCGGGAGGC CGAGGAGGCT CGCAGGCAGC TGGCCGCCTT GGGCCACACG
    GAACCCCCAC TCGTGGCTGA GGCCCCCTGG GCCCGCCGGC CTCTGGATCC GCGGCGTCGG
    AGCCTCCCTG CTGGCGATGC CCTGTACTTG AGTTTCACAC CCCCACAGCC CAGCCGAGGC
    CACGACCGCC TGGATTTGCC TGTAACTATT CGCTCTGTCC ATCGACCCTT TGAAGACCGA
    GAGAGGCAGG AGCTGGGCAG CCCCGACGAG CGGCTGCAAG ATAGCAGTGA CCCTGACACC
    GGCAGCGAGG AGGAGGGTAG CAGCCGTCTG TCTCCACCTC ATAGTCCACG AGATTTCACC
    CGAATGCAGG ACATCCCGGA AGAGACTGAG AGCCGAGATG GGGAGCCTGT AGCTTCTGAG
    AGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232571
    Clone ID Related Accession (Same CDS sequence) XM_024986248.1
    Accession Version XM_024986248.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGG GCCTGAAAGA CCTGCTGGTA GGGCCTGGAG TGGAGCTGCT CTTGACACCC
    CGGGAACCAC CCTTGCCCAT GGAGCCAGAC AGCGGTGGTA ACACGAGTCC TGGAGTCACT
    GCCAACGGTG AGGCCAGAAC CTTCAATGGA TCCATTGAGC TCTGCAGAGC CGACTCAGAC
    TCCAGCCAGA AGGATCGAAA TGGAAATCAG CTGAGATCTC CCCAGGAGGA AGCGTTGCAG
    CGATTGGTCA ATCTCTATGG ACTTCTGCAT GGCCTACAGG CAGCTGTGGC CCAGCAGGAC
    ACTCTGATGG AAGCCCGGTT CCCTGAGGGC CCCGAGCGGC GGGAGAAGCT GACCAGAGCC
    AATTCCCGGG ATGGGGAGGC TGGCAGAGCC GGGGGTGTCC CTGCGGTTCC TGAAAAGCAG
    GCTACGGAAC TGGCGTTGCT GCAGCGGCAA CACGCACTAT TGCAGGAGGA GCTGCGACGC
    TGCCGACGGC TCGGCGAGGA GCGTGCGACT GAGGCCGGCA GCTTGGAAGC CCGGCTCCGG
    GAGAGCGAGC AGGCCCGTGC CCTGCTGGAG CGGGAGGCCG AGGAGGCTCG CAGGCAGCTG
    GCCGCCTTGG GCCACACGGA ACCCCCACTC GTGGCTGAGG CCCCCTGGGC CCGCCGGCCT
    CTGGATCCGC GGCGTCGGAG CCTCCCTGCT GGCGATGCCC TGTACTTGAG TTTCACACCC
    CCACAGCCCA GCCGAGGCCA CGACCGCCTG GATTTGCCTG TAACTATTCG CTCTGTCCAT
    CGACCCTTTG AAGACCGAGA GAGGCAGGAG CTGGGCAGCC CCGACGAGCG GCTGCAAGAT
    AGCAGTGACC CTGACACCGG CAGCGAGGAG GAGGGTAGCA GCCGTCTGTC TCCACCTCAT
    AGTCCACGAG ATTTCACCCG AATGCAGGAC ATCCCGGAAG AGACTGAGAG CCGAGATGGG
    GAGCCTGTAG CTTCTGAGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842016.1
    CDS248..3631
    Translation

    Target ORF information:

    RefSeq Version XM_024986248.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986248.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGG GCCTGAAAGA CCTGCTGGTA GGGCCTGGAG TGGAGCTGCT CTTGACACCC
    CGGGAACCAC CCTTGCCCAT GGAGCCAGAC AGCGGTGGTA ACACGAGTCC TGGAGTCACT
    GCCAACGGTG AGGCCAGAAC CTTCAATGGA TCCATTGAGC TCTGCAGAGC CGACTCAGAC
    TCCAGCCAGA AGGATCGAAA TGGAAATCAG CTGAGATCTC CCCAGGAGGA AGCGTTGCAG
    CGATTGGTCA ATCTCTATGG ACTTCTGCAT GGCCTACAGG CAGCTGTGGC CCAGCAGGAC
    ACTCTGATGG AAGCCCGGTT CCCTGAGGGC CCCGAGCGGC GGGAGAAGCT GACCAGAGCC
    AATTCCCGGG ATGGGGAGGC TGGCAGAGCC GGGGGTGTCC CTGCGGTTCC TGAAAAGCAG
    GCTACGGAAC TGGCGTTGCT GCAGCGGCAA CACGCACTAT TGCAGGAGGA GCTGCGACGC
    TGCCGACGGC TCGGCGAGGA GCGTGCGACT GAGGCCGGCA GCTTGGAAGC CCGGCTCCGG
    GAGAGCGAGC AGGCCCGTGC CCTGCTGGAG CGGGAGGCCG AGGAGGCTCG CAGGCAGCTG
    GCCGCCTTGG GCCACACGGA ACCCCCACTC GTGGCTGAGG CCCCCTGGGC CCGCCGGCCT
    CTGGATCCGC GGCGTCGGAG CCTCCCTGCT GGCGATGCCC TGTACTTGAG TTTCACACCC
    CCACAGCCCA GCCGAGGCCA CGACCGCCTG GATTTGCCTG TAACTATTCG CTCTGTCCAT
    CGACCCTTTG AAGACCGAGA GAGGCAGGAG CTGGGCAGCC CCGACGAGCG GCTGCAAGAT
    AGCAGTGACC CTGACACCGG CAGCGAGGAG GAGGGTAGCA GCCGTCTGTC TCCACCTCAT
    AGTCCACGAG ATTTCACCCG AATGCAGGAC ATCCCGGAAG AGACTGAGAG CCGAGATGGG
    GAGCCTGTAG CTTCTGAGAG CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa232572
    Clone ID Related Accession (Same CDS sequence) XM_024986249.1
    Accession Version XM_024986249.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3261bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCACAAGC CCCAAGGAGC CCTCCTTACT GCGGAGCCAA GAGGAACTGG ACTCCTTTCT 
    GGACCTGCAA CACCAAGGTG GCCAACCCTC CTGCGTGATT CAGTTTTGGA TCAAGGTTCC
    TGTCACCCAC CAGCTGGCCC TTTGGACCAC TCAGCCACTG GTATGCTTTC CAAGTCTGTA
    TCCATGAGTG GCATCAATTG CTTCTTGGGC TGCTCAGACC CAGCTGGGAA TTTGTCCCAG
    TCTTCTGGCA CTGACCTGAG CCAAAGCTGT AGCCAACTGG AACGGAGCTC AGGTACCTGG
    GCAGGCTCCT CCCTGCGACG GACACTCAGC TTGATCTTGG GCATGACCGG AAAGGCCAAG
    ACCCGGGAAA AGGAGAAGAT GAAGGAAGCC AAGGACGCAC GCTACACCAA TGGGCACCTC
    TTCACCACCA TCTCCGTTTC GGGCATGACC ATGTGCTATG CCTGTAACAA GAGCATCACA
    GCCAAGGAAG CCCTCATCTG CCCAACCTGC AATGTGACTA TCCACAACCG CTGTAAAGAC
    ACCCTCGCCA ACTGTACCAA GGTCAAGCAG AAGCAACAGA AAGCTGCCCT GCTGAAGAAC
    AACACTGCCT TGCAGTCCGT TTCCCTTCGC AGTAAGACTA CCACTCGGGA GCGGCCCAGC
    TCAGCCATCT ACCCCTCCGA CATCCGGCAG TCCCTGCTCG GCTCCCGCCG TGGCCGCTCC
    TCCTTGTCTT TAGCCAAAAG TGTCTCCACC ACCAACATTG CTGGACACTT CAATGATGAG
    TCTCCCCTGG GGCTTCGCCG GATCCTCTCA CAGTCCACGG ACTCCCTCAA CATGCGGAAC
    CGGACACTGT CGGTGGAGTC CCTCATCGAC GAAGGTGCCG AGGTGATCTA CAACGAGCTG
    ATGAGTGACT TTGAGATGGA TGAGAAGGAC TTTACAGCCG ATTCCTGGAG CCTTGCAGTG
    GATAGCAGCT TCTTGCAGCA GCATAAAAAG GAGGTGATGA AGCAGCAGGA TGTCATCTAT
    GAGCTGATCC AGACAGAGCT ACACCACGTG CGGACGCTGA AGATCATGAC CCGCCTCTTC
    CGCACGGGCA TGCTGGAAGA GCTGCAGCTG GAGCCGGGAG TGGTCCAGGG CCTGTTCCCC
    TGTGTGGATG AGCTCACTGA CATCCACACG CGCTTCCTCA GCCAGCTGCT GGATCGCCGG
    CGCCAGGCCC TGTGCCCTGG CAGCACCCGG AACTTCGTCA TCCATCGTTT GGGGGACCTG
    CTCATCAGCC AGTTCTCAGG TCCTAGCGCA GAGCAGATGC GGAAGACCTA CTCAGAGTTC
    TGTAGCCGCC ACACCAAGGC CTTAAAACTC TATAAGGAGC TGTATGCCCG AGACAAACGC
    TTCCAGCAGT TCATCCGGAA AGTGACCCGC TCGGCGGTGC TGAAGCGGCA TGGGGTACAA
    GAGTGCATTC TGCTAGTGAC TCAGCGCATC ACCAAGTACC CGGTGCTCAT CAACCGCATC
    CTGCAGCATT CCCACGGGAT GGAGGAGGAG CGCCAGGACC TGACCAAGGC CCTGGGGCTG
    GTGAAGGAGC TGCTGTCCAA CGTGGACCAG GATGTGCACG AGCTGGAGAA AGGGGCCCGC
    CTGCAGGAGA TCTACAACCG CATGGACCCT CGGGCCCAGG CCCCGGTTCC TGGCAAGGGC
    CCCTTTGGCC GAGAGGAGCT CCTGCGGCGC AAGCTCATCC ACGATGGTTG CCTGCTCTGG
    AAGACGGCGA CTGGGCGCTT CAAAGATGTG CTCATGCTGC TGATGACAGA TGTGCTGGTG
    TTTCTCCAAG AGAAGGACCA GAAGTACATC TTTCCTGCCC TGGACAAGCC CTCGGTGGTG
    TCACTGCAGA ATCTCATCGT GCGGGACATC GCTAACCAGG AGAAAGGGAT GTTTCTGATC
    AGTGCGGCAC CCCCTGAGAT GTACGAGGTC CACACGGCAT CCCGGGATGA CCGGAGCACC
    TGGATCCGCG TCATTCAGCA GAGTGTGCGA GTATGCCCAT CCAGGGAGGA CTTCCCCCTG
    ATTGAGACAG AGGATGAGGC TTACCTCCGG CGTATCAAGA TGGAGCTGCA GCAGAAAGAC
    CGGGCACTGG TGGAGCTGCT GCAGGAGAAG GTTGGGCTAT TTGCCGAGAT GACCCACTTC
    CAGGTGGAAG AGGATGGCAG CAGCGGGGTG CCCCTGCCCA CCCTGCCCAG GGGCCTCTTC
    CGCTCTGAGT CCCTTGAGTC CCCTCGTGGC GAGCGGCTGC TGCAGGATGC CATCCGTGAG
    GTGGAGGGCC TGAAAGACCT GCTGGTAGGG CCTGGAGTGG AGCTGCTCTT GACACCCCGG
    GAACCACCCT TGCCCATGGA GCCAGACAGC GGTGGTAACA CGAGTCCTGG AGTCACTGCC
    AACGGTGAGG CCAGAACCTT CAATGGATCC ATTGAGCTCT GCAGAGCCGA CTCAGACTCC
    AGCCAGAAGG ATCGAAATGG AAATCAGCTG AGATCTCCCC AGGAGGAAGC GTTGCAGCGA
    TTGGTCAATC TCTATGGACT TCTGCATGGC CTACAGGCAG CTGTGGCCCA GCAGGACACT
    CTGATGGAAG CCCGGTTCCC TGAGGGCCCC GAGCGGCGGG AGAAGCTGAC CAGAGCCAAT
    TCCCGGGATG GGGAGGCTGG CAGAGCCGGG GGTGTCCCTG CGGTTCCTGA AAAGCAGGCT
    ACGGAACTGG CGTTGCTGCA GCGGCAACAC GCACTATTGC AGGAGGAGCT GCGACGCTGC
    CGACGGCTCG GCGAGGAGCG TGCGACTGAG GCCGGCAGCT TGGAAGCCCG GCTCCGGGAG
    AGCGAGCAGG CCCGTGCCCT GCTGGAGCGG GAGGCCGAGG AGGCTCGCAG GCAGCTGGCC
    GCCTTGGGCC ACACGGAACC CCCACTCGTG GCTGAGGCCC CCTGGGCCCG CCGGCCTCTG
    GATCCGCGGC GTCGGAGCCT CCCTGCTGGC GATGCCCTGT ACTTGAGTTT CACACCCCCA
    CAGCCCAGCC GAGGCCACGA CCGCCTGGAT TTGCCTGTAA CTATTCGCTC TGTCCATCGA
    CCCTTTGAAG ACCGAGAGAG GCAGGAGCTG GGCAGCCCCG ACGAGCGGCT GCAAGATAGC
    AGTGACCCTG ACACCGGCAG CGAGGAGGAG GGTAGCAGCC GTCTGTCTCC ACCTCATAGT
    CCACGAGATT TCACCCGAAT GCAGGACATC CCGGAAGAGA CTGAGAGCCG AGATGGGGAG
    CCTGTAGCTT CTGAGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024842017.1
    CDS544..3804
    Translation

    Target ORF information:

    RefSeq Version XM_024986249.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986249.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCACAAGC CCCAAGGAGC CCTCCTTACT GCGGAGCCAA GAGGAACTGG ACTCCTTTCT 
    GGACCTGCAA CACCAAGGTG GCCAACCCTC CTGCGTGATT CAGTTTTGGA TCAAGGTTCC
    TGTCACCCAC CAGCTGGCCC TTTGGACCAC TCAGCCACTG GTATGCTTTC CAAGTCTGTA
    TCCATGAGTG GCATCAATTG CTTCTTGGGC TGCTCAGACC CAGCTGGGAA TTTGTCCCAG
    TCTTCTGGCA CTGACCTGAG CCAAAGCTGT AGCCAACTGG AACGGAGCTC AGGTACCTGG
    GCAGGCTCCT CCCTGCGACG GACACTCAGC TTGATCTTGG GCATGACCGG AAAGGCCAAG
    ACCCGGGAAA AGGAGAAGAT GAAGGAAGCC AAGGACGCAC GCTACACCAA TGGGCACCTC
    TTCACCACCA TCTCCGTTTC GGGCATGACC ATGTGCTATG CCTGTAACAA GAGCATCACA
    GCCAAGGAAG CCCTCATCTG CCCAACCTGC AATGTGACTA TCCACAACCG CTGTAAAGAC
    ACCCTCGCCA ACTGTACCAA GGTCAAGCAG AAGCAACAGA AAGCTGCCCT GCTGAAGAAC
    AACACTGCCT TGCAGTCCGT TTCCCTTCGC AGTAAGACTA CCACTCGGGA GCGGCCCAGC
    TCAGCCATCT ACCCCTCCGA CATCCGGCAG TCCCTGCTCG GCTCCCGCCG TGGCCGCTCC
    TCCTTGTCTT TAGCCAAAAG TGTCTCCACC ACCAACATTG CTGGACACTT CAATGATGAG
    TCTCCCCTGG GGCTTCGCCG GATCCTCTCA CAGTCCACGG ACTCCCTCAA CATGCGGAAC
    CGGACACTGT CGGTGGAGTC CCTCATCGAC GAAGGTGCCG AGGTGATCTA CAACGAGCTG
    ATGAGTGACT TTGAGATGGA TGAGAAGGAC TTTACAGCCG ATTCCTGGAG CCTTGCAGTG
    GATAGCAGCT TCTTGCAGCA GCATAAAAAG GAGGTGATGA AGCAGCAGGA TGTCATCTAT
    GAGCTGATCC AGACAGAGCT ACACCACGTG CGGACGCTGA AGATCATGAC CCGCCTCTTC
    CGCACGGGCA TGCTGGAAGA GCTGCAGCTG GAGCCGGGAG TGGTCCAGGG CCTGTTCCCC
    TGTGTGGATG AGCTCACTGA CATCCACACG CGCTTCCTCA GCCAGCTGCT GGATCGCCGG
    CGCCAGGCCC TGTGCCCTGG CAGCACCCGG AACTTCGTCA TCCATCGTTT GGGGGACCTG
    CTCATCAGCC AGTTCTCAGG TCCTAGCGCA GAGCAGATGC GGAAGACCTA CTCAGAGTTC
    TGTAGCCGCC ACACCAAGGC CTTAAAACTC TATAAGGAGC TGTATGCCCG AGACAAACGC
    TTCCAGCAGT TCATCCGGAA AGTGACCCGC TCGGCGGTGC TGAAGCGGCA TGGGGTACAA
    GAGTGCATTC TGCTAGTGAC TCAGCGCATC ACCAAGTACC CGGTGCTCAT CAACCGCATC
    CTGCAGCATT CCCACGGGAT GGAGGAGGAG CGCCAGGACC TGACCAAGGC CCTGGGGCTG
    GTGAAGGAGC TGCTGTCCAA CGTGGACCAG GATGTGCACG AGCTGGAGAA AGGGGCCCGC
    CTGCAGGAGA TCTACAACCG CATGGACCCT CGGGCCCAGG CCCCGGTTCC TGGCAAGGGC
    CCCTTTGGCC GAGAGGAGCT CCTGCGGCGC AAGCTCATCC ACGATGGTTG CCTGCTCTGG
    AAGACGGCGA CTGGGCGCTT CAAAGATGTG CTCATGCTGC TGATGACAGA TGTGCTGGTG
    TTTCTCCAAG AGAAGGACCA GAAGTACATC TTTCCTGCCC TGGACAAGCC CTCGGTGGTG
    TCACTGCAGA ATCTCATCGT GCGGGACATC GCTAACCAGG AGAAAGGGAT GTTTCTGATC
    AGTGCGGCAC CCCCTGAGAT GTACGAGGTC CACACGGCAT CCCGGGATGA CCGGAGCACC
    TGGATCCGCG TCATTCAGCA GAGTGTGCGA GTATGCCCAT CCAGGGAGGA CTTCCCCCTG
    ATTGAGACAG AGGATGAGGC TTACCTCCGG CGTATCAAGA TGGAGCTGCA GCAGAAAGAC
    CGGGCACTGG TGGAGCTGCT GCAGGAGAAG GTTGGGCTAT TTGCCGAGAT GACCCACTTC
    CAGGTGGAAG AGGATGGCAG CAGCGGGGTG CCCCTGCCCA CCCTGCCCAG GGGCCTCTTC
    CGCTCTGAGT CCCTTGAGTC CCCTCGTGGC GAGCGGCTGC TGCAGGATGC CATCCGTGAG
    GTGGAGGGCC TGAAAGACCT GCTGGTAGGG CCTGGAGTGG AGCTGCTCTT GACACCCCGG
    GAACCACCCT TGCCCATGGA GCCAGACAGC GGTGGTAACA CGAGTCCTGG AGTCACTGCC
    AACGGTGAGG CCAGAACCTT CAATGGATCC ATTGAGCTCT GCAGAGCCGA CTCAGACTCC
    AGCCAGAAGG ATCGAAATGG AAATCAGCTG AGATCTCCCC AGGAGGAAGC GTTGCAGCGA
    TTGGTCAATC TCTATGGACT TCTGCATGGC CTACAGGCAG CTGTGGCCCA GCAGGACACT
    CTGATGGAAG CCCGGTTCCC TGAGGGCCCC GAGCGGCGGG AGAAGCTGAC CAGAGCCAAT
    TCCCGGGATG GGGAGGCTGG CAGAGCCGGG GGTGTCCCTG CGGTTCCTGA AAAGCAGGCT
    ACGGAACTGG CGTTGCTGCA GCGGCAACAC GCACTATTGC AGGAGGAGCT GCGACGCTGC
    CGACGGCTCG GCGAGGAGCG TGCGACTGAG GCCGGCAGCT TGGAAGCCCG GCTCCGGGAG
    AGCGAGCAGG CCCGTGCCCT GCTGGAGCGG GAGGCCGAGG AGGCTCGCAG GCAGCTGGCC
    GCCTTGGGCC ACACGGAACC CCCACTCGTG GCTGAGGCCC CCTGGGCCCG CCGGCCTCTG
    GATCCGCGGC GTCGGAGCCT CCCTGCTGGC GATGCCCTGT ACTTGAGTTT CACACCCCCA
    CAGCCCAGCC GAGGCCACGA CCGCCTGGAT TTGCCTGTAA CTATTCGCTC TGTCCATCGA
    CCCTTTGAAG ACCGAGAGAG GCAGGAGCTG GGCAGCCCCG ACGAGCGGCT GCAAGATAGC
    AGTGACCCTG ACACCGGCAG CGAGGAGGAG GGTAGCAGCC GTCTGTCTCC ACCTCATAGT
    CCACGAGATT TCACCCGAAT GCAGGACATC CCGGAAGAGA CTGAGAGCCG AGATGGGGAG
    CCTGTAGCTT CTGAGAGCTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88159
    Clone ID Related Accession (Same CDS sequence) XM_005203664.3
    Accession Version XM_005203664.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3129bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005203664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTTATAA GAGGAGCTAA CTCCCATAGA TTTGAAAGCG CTGGTGAGCT GGCACCTTCT 
    GCTTTGCCTG CTCTCCCCTC CTCTCTGCGC ATCTTCTCCC GGCACAGAGA AGAGAGTACA
    GCAGCTTGGG GGCGAGCAGA GCTAGAGCTG GGAGGTGGAG TGAGGACGCG GAGGTCTAGA
    GCTGTGGAGA TGAGCGGCAA CAGGAGGCAA CCCAGCCGCA GGGGCCAGAC CCGGGAAAAG
    GAGAAGATGA AGGAAGCCAA GGACGCACGC TACACCAATG GGCACCTCTT CACCACCATC
    TCCGTTTCGG GCATGACCAT GTGCTATGCC TGTAACAAGA GCATCACAGC CAAGGAAGCC
    CTCATCTGCC CAACCTGCAA TGTGACTATC CACAACCGCT GTAAAGACAC CCTCGCCAAC
    TGTACCAAGG TCAAGCAGAA GCAACAGAAA GCTGCCCTGC TGAAGAACAA CACTGCCTTG
    CAGTCCGTTT CCCTTCGCAG TAAGACTACC ACTCGGGAGC GGCCCAGCTC AGCCATCTAC
    CCCTCCGACA TCCGGCAGTC CCTGCTCGGC TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA
    GCCAAAAGTG TCTCCACCAC CAACATTGCT GGACACTTCA ATGATGAGTC TCCCCTGGGG
    CTTCGCCGGA TCCTCTCACA GTCCACGGAC TCCCTCAACA TGCGGAACCG GACACTGTCG
    GTGGAGTCCC TCATCGACGA AGGTGCCGAG GTGATCTACA ACGAGCTGAT GAGTGACTTT
    GAGATGGATG AGAAGGACTT TACAGCCGAT TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC
    TTGCAGCAGC ATAAAAAGGA GGTGATGAAG CAGCAGGATG TCATCTATGA GCTGATCCAG
    ACAGAGCTAC ACCACGTGCG GACGCTGAAG ATCATGACCC GCCTCTTCCG CACGGGCATG
    CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG GTCCAGGGCC TGTTCCCCTG TGTGGATGAG
    CTCACTGACA TCCACACGCG CTTCCTCAGC CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG
    TGCCCTGGCA GCACCCGGAA CTTCGTCATC CATCGTTTGG GGGACCTGCT CATCAGCCAG
    TTCTCAGGTC CTAGCGCAGA GCAGATGCGG AAGACCTACT CAGAGTTCTG TAGCCGCCAC
    ACCAAGGCCT TAAAACTCTA TAAGGAGCTG TATGCCCGAG ACAAACGCTT CCAGCAGTTC
    ATCCGGAAAG TGACCCGCTC GGCGGTGCTG AAGCGGCATG GGGTACAAGA GTGCATTCTG
    CTAGTGACTC AGCGCATCAC CAAGTACCCG GTGCTCATCA ACCGCATCCT GCAGCATTCC
    CACGGGATGG AGGAGGAGCG CCAGGACCTG ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG
    CTGTCCAACG TGGACCAGGA TGTGCACGAG CTGGAGAAAG GGGCCCGCCT GCAGGAGATC
    TACAACCGCA TGGACCCTCG GGCCCAGGCC CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA
    GAGGAGCTCC TGCGGCGCAA GCTCATCCAC GATGGTTGCC TGCTCTGGAA GACGGCGACT
    GGGCGCTTCA AAGATGTGCT CATGCTGCTG ATGACAGATG TGCTGGTGTT TCTCCAAGAG
    AAGGACCAGA AGTACATCTT TCCTGCCCTG GACAAGCCCT CGGTGGTGTC ACTGCAGAAT
    CTCATCGTGC GGGACATCGC TAACCAGGAG AAAGGGATGT TTCTGATCAG TGCGGCACCC
    CCTGAGATGT ACGAGGTCCA CACGGCATCC CGGGATGACC GGAGCACCTG GATCCGCGTC
    ATTCAGCAGA GTGTGCGAGT ATGCCCATCC AGGGAGGACT TCCCCCTGAT TGAGACAGAG
    GATGAGGCTT ACCTCCGGCG TATCAAGATG GAGCTGCAGC AGAAAGACCG GGCACTGGTG
    GAGCTGCTGC AGGAGAAGGT TGGGCTATTT GCCGAGATGA CCCACTTCCA GGTGGAAGAG
    GATGGCAGCA GCGGGGTGCC CCTGCCCACC CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC
    CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG CAGGATGCCA TCCGTGAGGT GGAGGGCCTG
    AAAGACCTGC TGGTAGGGCC TGGAGTGGAG CTGCTCTTGA CACCCCGGGA ACCACCCTTG
    CCCATGGAGC CAGACAGCGG TGGTAACACG AGTCCTGGAG TCACTGCCAA CGGTGAGGCC
    AGAACCTTCA ATGGATCCAT TGAGCTCTGC AGAGCCGACT CAGACTCCAG CCAGAAGGAT
    CGAAATGGAA ATCAGCTGAG ATCTCCCCAG GAGGAAGCGT TGCAGCGATT GGTCAATCTC
    TATGGACTTC TGCATGGCCT ACAGGCAGCT GTGGCCCAGC AGGACACTCT GATGGAAGCC
    CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG AAGCTGACCA GAGCCAATTC CCGGGATGGG
    GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG GTTCCTGAAA AGCAGGCTAC GGAACTGGCG
    TTGCTGCAGC GGCAACACGC ACTATTGCAG GAGGAGCTGC GACGCTGCCG ACGGCTCGGC
    GAGGAGCGTG CGACTGAGGC CGGCAGCTTG GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC
    CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC
    ACGGAACCCC CACTCGTGGC TGAGGCCCCC TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT
    CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC TTGAGTTTCA CACCCCCACA GCCCAGCCGA
    GGCCACGACC GCCTGGATTT GCCTGTAACT ATTCGCTCTG TCCATCGACC CTTTGAAGAC
    CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC GAGCGGCTGC AAGATAGCAG TGACCCTGAC
    ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT CTGTCTCCAC CTCATAGTCC ACGAGATTTC
    ACCCGAATGC AGGACATCCC GGAAGAGACT GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT
    GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005203721.3
    CDS661..3789
    Translation

    Target ORF information:

    RefSeq Version XM_005203664.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005203664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGTTATAA GAGGAGCTAA CTCCCATAGA TTTGAAAGCG CTGGTGAGCT GGCACCTTCT 
    GCTTTGCCTG CTCTCCCCTC CTCTCTGCGC ATCTTCTCCC GGCACAGAGA AGAGAGTACA
    GCAGCTTGGG GGCGAGCAGA GCTAGAGCTG GGAGGTGGAG TGAGGACGCG GAGGTCTAGA
    GCTGTGGAGA TGAGCGGCAA CAGGAGGCAA CCCAGCCGCA GGGGCCAGAC CCGGGAAAAG
    GAGAAGATGA AGGAAGCCAA GGACGCACGC TACACCAATG GGCACCTCTT CACCACCATC
    TCCGTTTCGG GCATGACCAT GTGCTATGCC TGTAACAAGA GCATCACAGC CAAGGAAGCC
    CTCATCTGCC CAACCTGCAA TGTGACTATC CACAACCGCT GTAAAGACAC CCTCGCCAAC
    TGTACCAAGG TCAAGCAGAA GCAACAGAAA GCTGCCCTGC TGAAGAACAA CACTGCCTTG
    CAGTCCGTTT CCCTTCGCAG TAAGACTACC ACTCGGGAGC GGCCCAGCTC AGCCATCTAC
    CCCTCCGACA TCCGGCAGTC CCTGCTCGGC TCCCGCCGTG GCCGCTCCTC CTTGTCTTTA
    GCCAAAAGTG TCTCCACCAC CAACATTGCT GGACACTTCA ATGATGAGTC TCCCCTGGGG
    CTTCGCCGGA TCCTCTCACA GTCCACGGAC TCCCTCAACA TGCGGAACCG GACACTGTCG
    GTGGAGTCCC TCATCGACGA AGGTGCCGAG GTGATCTACA ACGAGCTGAT GAGTGACTTT
    GAGATGGATG AGAAGGACTT TACAGCCGAT TCCTGGAGCC TTGCAGTGGA TAGCAGCTTC
    TTGCAGCAGC ATAAAAAGGA GGTGATGAAG CAGCAGGATG TCATCTATGA GCTGATCCAG
    ACAGAGCTAC ACCACGTGCG GACGCTGAAG ATCATGACCC GCCTCTTCCG CACGGGCATG
    CTGGAAGAGC TGCAGCTGGA GCCGGGAGTG GTCCAGGGCC TGTTCCCCTG TGTGGATGAG
    CTCACTGACA TCCACACGCG CTTCCTCAGC CAGCTGCTGG ATCGCCGGCG CCAGGCCCTG
    TGCCCTGGCA GCACCCGGAA CTTCGTCATC CATCGTTTGG GGGACCTGCT CATCAGCCAG
    TTCTCAGGTC CTAGCGCAGA GCAGATGCGG AAGACCTACT CAGAGTTCTG TAGCCGCCAC
    ACCAAGGCCT TAAAACTCTA TAAGGAGCTG TATGCCCGAG ACAAACGCTT CCAGCAGTTC
    ATCCGGAAAG TGACCCGCTC GGCGGTGCTG AAGCGGCATG GGGTACAAGA GTGCATTCTG
    CTAGTGACTC AGCGCATCAC CAAGTACCCG GTGCTCATCA ACCGCATCCT GCAGCATTCC
    CACGGGATGG AGGAGGAGCG CCAGGACCTG ACCAAGGCCC TGGGGCTGGT GAAGGAGCTG
    CTGTCCAACG TGGACCAGGA TGTGCACGAG CTGGAGAAAG GGGCCCGCCT GCAGGAGATC
    TACAACCGCA TGGACCCTCG GGCCCAGGCC CCGGTTCCTG GCAAGGGCCC CTTTGGCCGA
    GAGGAGCTCC TGCGGCGCAA GCTCATCCAC GATGGTTGCC TGCTCTGGAA GACGGCGACT
    GGGCGCTTCA AAGATGTGCT CATGCTGCTG ATGACAGATG TGCTGGTGTT TCTCCAAGAG
    AAGGACCAGA AGTACATCTT TCCTGCCCTG GACAAGCCCT CGGTGGTGTC ACTGCAGAAT
    CTCATCGTGC GGGACATCGC TAACCAGGAG AAAGGGATGT TTCTGATCAG TGCGGCACCC
    CCTGAGATGT ACGAGGTCCA CACGGCATCC CGGGATGACC GGAGCACCTG GATCCGCGTC
    ATTCAGCAGA GTGTGCGAGT ATGCCCATCC AGGGAGGACT TCCCCCTGAT TGAGACAGAG
    GATGAGGCTT ACCTCCGGCG TATCAAGATG GAGCTGCAGC AGAAAGACCG GGCACTGGTG
    GAGCTGCTGC AGGAGAAGGT TGGGCTATTT GCCGAGATGA CCCACTTCCA GGTGGAAGAG
    GATGGCAGCA GCGGGGTGCC CCTGCCCACC CTGCCCAGGG GCCTCTTCCG CTCTGAGTCC
    CTTGAGTCCC CTCGTGGCGA GCGGCTGCTG CAGGATGCCA TCCGTGAGGT GGAGGGCCTG
    AAAGACCTGC TGGTAGGGCC TGGAGTGGAG CTGCTCTTGA CACCCCGGGA ACCACCCTTG
    CCCATGGAGC CAGACAGCGG TGGTAACACG AGTCCTGGAG TCACTGCCAA CGGTGAGGCC
    AGAACCTTCA ATGGATCCAT TGAGCTCTGC AGAGCCGACT CAGACTCCAG CCAGAAGGAT
    CGAAATGGAA ATCAGCTGAG ATCTCCCCAG GAGGAAGCGT TGCAGCGATT GGTCAATCTC
    TATGGACTTC TGCATGGCCT ACAGGCAGCT GTGGCCCAGC AGGACACTCT GATGGAAGCC
    CGGTTCCCTG AGGGCCCCGA GCGGCGGGAG AAGCTGACCA GAGCCAATTC CCGGGATGGG
    GAGGCTGGCA GAGCCGGGGG TGTCCCTGCG GTTCCTGAAA AGCAGGCTAC GGAACTGGCG
    TTGCTGCAGC GGCAACACGC ACTATTGCAG GAGGAGCTGC GACGCTGCCG ACGGCTCGGC
    GAGGAGCGTG CGACTGAGGC CGGCAGCTTG GAAGCCCGGC TCCGGGAGAG CGAGCAGGCC
    CGTGCCCTGC TGGAGCGGGA GGCCGAGGAG GCTCGCAGGC AGCTGGCCGC CTTGGGCCAC
    ACGGAACCCC CACTCGTGGC TGAGGCCCCC TGGGCCCGCC GGCCTCTGGA TCCGCGGCGT
    CGGAGCCTCC CTGCTGGCGA TGCCCTGTAC TTGAGTTTCA CACCCCCACA GCCCAGCCGA
    GGCCACGACC GCCTGGATTT GCCTGTAACT ATTCGCTCTG TCCATCGACC CTTTGAAGAC
    CGAGAGAGGC AGGAGCTGGG CAGCCCCGAC GAGCGGCTGC AAGATAGCAG TGACCCTGAC
    ACCGGCAGCG AGGAGGAGGG TAGCAGCCGT CTGTCTCCAC CTCATAGTCC ACGAGATTTC
    ACCCGAATGC AGGACATCCC GGAAGAGACT GAGAGCCGAG ATGGGGAGCC TGTAGCTTCT
    GAGAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46394
    Clone ID Related Accession (Same CDS sequence) XM_010802880.2 , XM_010802880.3
    Accession Version XM_010802880.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103861.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802880.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGC TGCAGCAGAA AGACCGGGCA CTGGTGGAGC TGCTGCAGGA GAAGGTTGGG
    CTATTTGCCG AGATGACCCA CTTCCAGGTG GAAGAGGATG GCAGCAGCGG GGTGCCCCTG
    CCCACCCTGC CCAGGGGCCT CTTCCGCTCT GAGTCCCTTG AGTCCCCTCG TGGCGAGCGG
    CTGCTGCAGG ATGCCATCCG TGAGGTGGAG GGCCTGAAAG ACCTGCTGGT AGGGCCTGGA
    GTGGAGCTGC TCTTGACACC CCGGGAACCA CCCTTGCCCA TGGAGCCAGA CAGCGGTGGT
    AACACGAGTC CTGGAGTCAC TGCCAACGGT GAGGCCAGAA CCTTCAATGG ATCCATTGAG
    CTCTGCAGAG CCGACTCAGA CTCCAGCCAG AAGGATCGAA ATGGAAATCA GCTGAGATCT
    CCCCAGGAGG AAGCGTTGCA GCGATTGGTC AATCTCTATG GACTTCTGCA TGGCCTACAG
    GCAGCTGTGG CCCAGCAGGA CACTCTGATG GAAGCCCGGT TCCCTGAGGG CCCCGAGCGG
    CGGGAGAAGC TGACCAGAGC CAATTCCCGG GATGGGGAGG CTGGCAGAGC CGGGGGTGTC
    CCTGCGGTTC CTGAAAAGCA GGCTACGGAA CTGGCGTTGC TGCAGCGGCA ACACGCACTA
    TTGCAGGAGG AGCTGCGACG CTGCCGACGG CTCGGCGAGG AGCGTGCGAC TGAGGCCGGC
    AGCTTGGAAG CCCGGCTCCG GGAGAGCGAG CAGGCCCGTG CCCTGCTGGA GCGGGAGGCC
    GAGGAGGCTC GCAGGCAGCT GGCCGCCTTG GGCCACACGG AACCCCCACT CGTGGCTGAG
    GCCCCCTGGG CCCGCCGGCC TCTGGATCCG CGGCGTCGGA GCCTCCCTGC TGGCGATGCC
    CTGTACTTGA GTTTCACACC CCCACAGCCC AGCCGAGGCC ACGACCGCCT GGATTTGCCT
    GTAACTATTC GCTCTGTCCA TCGACCCTTT GAAGACCGAG AGAGGCAGGA GCTGGGCAGC
    CCCGACGAGC GGCTGCAAGA TAGCAGTGAC CCTGACACCG GCAGCGAGGA GGAGGGTAGC
    AGCCGTCTGT CTCCACCTCA TAGTCCACGA GATTTCACCC GAATGCAGGA CATCCCGGAA
    GAGACTGAGA GCCGAGATGG GGAGCCTGTA GCTTCTGAGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801182.1
    CDS248..3832
    Translation

    Target ORF information:

    RefSeq Version XM_010802880.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802880.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGC TGCAGCAGAA AGACCGGGCA CTGGTGGAGC TGCTGCAGGA GAAGGTTGGG
    CTATTTGCCG AGATGACCCA CTTCCAGGTG GAAGAGGATG GCAGCAGCGG GGTGCCCCTG
    CCCACCCTGC CCAGGGGCCT CTTCCGCTCT GAGTCCCTTG AGTCCCCTCG TGGCGAGCGG
    CTGCTGCAGG ATGCCATCCG TGAGGTGGAG GGCCTGAAAG ACCTGCTGGT AGGGCCTGGA
    GTGGAGCTGC TCTTGACACC CCGGGAACCA CCCTTGCCCA TGGAGCCAGA CAGCGGTGGT
    AACACGAGTC CTGGAGTCAC TGCCAACGGT GAGGCCAGAA CCTTCAATGG ATCCATTGAG
    CTCTGCAGAG CCGACTCAGA CTCCAGCCAG AAGGATCGAA ATGGAAATCA GCTGAGATCT
    CCCCAGGAGG AAGCGTTGCA GCGATTGGTC AATCTCTATG GACTTCTGCA TGGCCTACAG
    GCAGCTGTGG CCCAGCAGGA CACTCTGATG GAAGCCCGGT TCCCTGAGGG CCCCGAGCGG
    CGGGAGAAGC TGACCAGAGC CAATTCCCGG GATGGGGAGG CTGGCAGAGC CGGGGGTGTC
    CCTGCGGTTC CTGAAAAGCA GGCTACGGAA CTGGCGTTGC TGCAGCGGCA ACACGCACTA
    TTGCAGGAGG AGCTGCGACG CTGCCGACGG CTCGGCGAGG AGCGTGCGAC TGAGGCCGGC
    AGCTTGGAAG CCCGGCTCCG GGAGAGCGAG CAGGCCCGTG CCCTGCTGGA GCGGGAGGCC
    GAGGAGGCTC GCAGGCAGCT GGCCGCCTTG GGCCACACGG AACCCCCACT CGTGGCTGAG
    GCCCCCTGGG CCCGCCGGCC TCTGGATCCG CGGCGTCGGA GCCTCCCTGC TGGCGATGCC
    CTGTACTTGA GTTTCACACC CCCACAGCCC AGCCGAGGCC ACGACCGCCT GGATTTGCCT
    GTAACTATTC GCTCTGTCCA TCGACCCTTT GAAGACCGAG AGAGGCAGGA GCTGGGCAGC
    CCCGACGAGC GGCTGCAAGA TAGCAGTGAC CCTGACACCG GCAGCGAGGA GGAGGGTAGC
    AGCCGTCTGT CTCCACCTCA TAGTCCACGA GATTTCACCC GAATGCAGGA CATCCCGGAA
    GAGACTGAGA GCCGAGATGG GGAGCCTGTA GCTTCTGAGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46394
    Clone ID Related Accession (Same CDS sequence) XM_010802880.2 , XM_010802880.3
    Accession Version XM_010802880.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product rho guanine nucleotide exchange factor 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010802880.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGC TGCAGCAGAA AGACCGGGCA CTGGTGGAGC TGCTGCAGGA GAAGGTTGGG
    CTATTTGCCG AGATGACCCA CTTCCAGGTG GAAGAGGATG GCAGCAGCGG GGTGCCCCTG
    CCCACCCTGC CCAGGGGCCT CTTCCGCTCT GAGTCCCTTG AGTCCCCTCG TGGCGAGCGG
    CTGCTGCAGG ATGCCATCCG TGAGGTGGAG GGCCTGAAAG ACCTGCTGGT AGGGCCTGGA
    GTGGAGCTGC TCTTGACACC CCGGGAACCA CCCTTGCCCA TGGAGCCAGA CAGCGGTGGT
    AACACGAGTC CTGGAGTCAC TGCCAACGGT GAGGCCAGAA CCTTCAATGG ATCCATTGAG
    CTCTGCAGAG CCGACTCAGA CTCCAGCCAG AAGGATCGAA ATGGAAATCA GCTGAGATCT
    CCCCAGGAGG AAGCGTTGCA GCGATTGGTC AATCTCTATG GACTTCTGCA TGGCCTACAG
    GCAGCTGTGG CCCAGCAGGA CACTCTGATG GAAGCCCGGT TCCCTGAGGG CCCCGAGCGG
    CGGGAGAAGC TGACCAGAGC CAATTCCCGG GATGGGGAGG CTGGCAGAGC CGGGGGTGTC
    CCTGCGGTTC CTGAAAAGCA GGCTACGGAA CTGGCGTTGC TGCAGCGGCA ACACGCACTA
    TTGCAGGAGG AGCTGCGACG CTGCCGACGG CTCGGCGAGG AGCGTGCGAC TGAGGCCGGC
    AGCTTGGAAG CCCGGCTCCG GGAGAGCGAG CAGGCCCGTG CCCTGCTGGA GCGGGAGGCC
    GAGGAGGCTC GCAGGCAGCT GGCCGCCTTG GGCCACACGG AACCCCCACT CGTGGCTGAG
    GCCCCCTGGG CCCGCCGGCC TCTGGATCCG CGGCGTCGGA GCCTCCCTGC TGGCGATGCC
    CTGTACTTGA GTTTCACACC CCCACAGCCC AGCCGAGGCC ACGACCGCCT GGATTTGCCT
    GTAACTATTC GCTCTGTCCA TCGACCCTTT GAAGACCGAG AGAGGCAGGA GCTGGGCAGC
    CCCGACGAGC GGCTGCAAGA TAGCAGTGAC CCTGACACCG GCAGCGAGGA GGAGGGTAGC
    AGCCGTCTGT CTCCACCTCA TAGTCCACGA GATTTCACCC GAATGCAGGA CATCCCGGAA
    GAGACTGAGA GCCGAGATGG GGAGCCTGTA GCTTCTGAGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010801182.1
    CDS248..3832
    Translation

    Target ORF information:

    RefSeq Version XM_010802880.3
    Organism Bos taurus(cattle)
    Definition Bos taurus Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802880.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAACAGAG AAGTCCCAGA GGTGTTCCTC GCAGGTGGAG ATCTGGTGCC CCGAGGCCAC 
    ACTCGCCGCT GCCTCTCTGC GGTGGAGTCC AGAGCGCAGC TGGAGGAAGA GGAAGAGGAG
    GAGAATCAGA GGGAGGGGAG AAAAAAGGAG GGCGAAGCCT TCCATGCTGT GCCCCTCCGA
    AGGTCGGGCG CTTTTCGTGC CCACAGCCAC AACTACGACC CCTTCAAAAG GCATAGCTGG
    GGGCCGGGCA GGGAGCTCCA GGATCCACTG AACAGGAATA AACTGCAGGC ATCGGGATGC
    ACAAGCCCCA AGGAGCCCTC CTTACTGCGG AGCCAAGAGG AACTGGACTC CTTTCTGGAC
    CTGCAACACC AAGGTGGCCA ACCCTCCTGC GTGGACCATT CAGACTTTCT ATTTCTTCTA
    GATTCAGTTT TGGATCAAGG TTCCTGTCAC CCACCAGCTG GCCCTTTGGA CCACTCAGCC
    ACTGGTATGC TTTCCAAGTC TGTATCCATG AGTGGCATCA ATTGCTTCTT GGGCTGCTCA
    GACCCAGCTG GGAATTTGTC CCAGTCTTCT GGCACTGACC TGAGCCAAAG CTGTAGCCAA
    CTGGAACGGA GCTCAGGTAC CTGGGCAGGC TCCTCCCTGC GACGGACACT CAGCTTGATC
    TTGGGCATGA CCGGAAAGGC CAAGACCCGG GAAAAGGAGA AGATGAAGGA AGCCAAGGAC
    GCACGCTACA CCAATGGGCA CCTCTTCACC ACCATCTCCG TTTCGGGCAT GACCATGTGC
    TATGCCTGTA ACAAGAGCAT CACAGCCAAG GAAGCCCTCA TCTGCCCAAC CTGCAATGTG
    ACTATCCACA ACCGCTGTAA AGACACCCTC GCCAACTGTA CCAAGGTCAA GCAGAAGCAA
    CAGAAAGCTG CCCTGCTGAA GAACAACACT GCCTTGCAGT CCGTTTCCCT TCGCAGTAAG
    ACTACCACTC GGGAGCGGCC CAGCTCAGCC ATCTACCCCT CCGACATCCG GCAGTCCCTG
    CTCGGCTCCC GCCGTGGCCG CTCCTCCTTG TCTTTAGCCA AAAGTGTCTC CACCACCAAC
    ATTGCTGGAC ACTTCAATGA TGAGTCTCCC CTGGGGCTTC GCCGGATCCT CTCACAGTCC
    ACGGACTCCC TCAACATGCG GAACCGGACA CTGTCGGTGG AGTCCCTCAT CGACGAAGGT
    GCCGAGGTGA TCTACAACGA GCTGATGAGT GACTTTGAGA TGGATGAGAA GGACTTTACA
    GCCGATTCCT GGAGCCTTGC AGTGGATAGC AGCTTCTTGC AGCAGCATAA AAAGGAGGTG
    ATGAAGCAGC AGGATGTCAT CTATGAGCTG ATCCAGACAG AGCTACACCA CGTGCGGACG
    CTGAAGATCA TGACCCGCCT CTTCCGCACG GGCATGCTGG AAGAGCTGCA GCTGGAGCCG
    GGAGTGGTCC AGGGCCTGTT CCCCTGTGTG GATGAGCTCA CTGACATCCA CACGCGCTTC
    CTCAGCCAGC TGCTGGATCG CCGGCGCCAG GCCCTGTGCC CTGGCAGCAC CCGGAACTTC
    GTCATCCATC GTTTGGGGGA CCTGCTCATC AGCCAGTTCT CAGGTCCTAG CGCAGAGCAG
    ATGCGGAAGA CCTACTCAGA GTTCTGTAGC CGCCACACCA AGGCCTTAAA ACTCTATAAG
    GAGCTGTATG CCCGAGACAA ACGCTTCCAG CAGTTCATCC GGAAAGTGAC CCGCTCGGCG
    GTGCTGAAGC GGCATGGGGT ACAAGAGTGC ATTCTGCTAG TGACTCAGCG CATCACCAAG
    TACCCGGTGC TCATCAACCG CATCCTGCAG CATTCCCACG GGATGGAGGA GGAGCGCCAG
    GACCTGACCA AGGCCCTGGG GCTGGTGAAG GAGCTGCTGT CCAACGTGGA CCAGGATGTG
    CACGAGCTGG AGAAAGGGGC CCGCCTGCAG GAGATCTACA ACCGCATGGA CCCTCGGGCC
    CAGGCCCCGG TTCCTGGCAA GGGCCCCTTT GGCCGAGAGG AGCTCCTGCG GCGCAAGCTC
    ATCCACGATG GTTGCCTGCT CTGGAAGACG GCGACTGGGC GCTTCAAAGA TGTGCTCATG
    CTGCTGATGA CAGATGTGCT GGTGTTTCTC CAAGAGAAGG ACCAGAAGTA CATCTTTCCT
    GCCCTGGACA AGCCCTCGGT GGTGTCACTG CAGAATCTCA TCGTGCGGGA CATCGCTAAC
    CAGGAGAAAG GGATGTTTCT GATCAGTGCG GCACCCCCTG AGATGTACGA GGTCCACACG
    GCATCCCGGG ATGACCGGAG CACCTGGATC CGCGTCATTC AGCAGAGTGT GCGAGTATGC
    CCATCCAGGG AGGACTTCCC CCTGATTGAG ACAGAGGATG AGGCTTACCT CCGGCGTATC
    AAGATGGAGC TGCAGCAGAA AGACCGGGCA CTGGTGGAGC TGCTGCAGGA GAAGGTTGGG
    CTATTTGCCG AGATGACCCA CTTCCAGGTG GAAGAGGATG GCAGCAGCGG GGTGCCCCTG
    CCCACCCTGC CCAGGGGCCT CTTCCGCTCT GAGTCCCTTG AGTCCCCTCG TGGCGAGCGG
    CTGCTGCAGG ATGCCATCCG TGAGGTGGAG GGCCTGAAAG ACCTGCTGGT AGGGCCTGGA
    GTGGAGCTGC TCTTGACACC CCGGGAACCA CCCTTGCCCA TGGAGCCAGA CAGCGGTGGT
    AACACGAGTC CTGGAGTCAC TGCCAACGGT GAGGCCAGAA CCTTCAATGG ATCCATTGAG
    CTCTGCAGAG CCGACTCAGA CTCCAGCCAG AAGGATCGAA ATGGAAATCA GCTGAGATCT
    CCCCAGGAGG AAGCGTTGCA GCGATTGGTC AATCTCTATG GACTTCTGCA TGGCCTACAG
    GCAGCTGTGG CCCAGCAGGA CACTCTGATG GAAGCCCGGT TCCCTGAGGG CCCCGAGCGG
    CGGGAGAAGC TGACCAGAGC CAATTCCCGG GATGGGGAGG CTGGCAGAGC CGGGGGTGTC
    CCTGCGGTTC CTGAAAAGCA GGCTACGGAA CTGGCGTTGC TGCAGCGGCA ACACGCACTA
    TTGCAGGAGG AGCTGCGACG CTGCCGACGG CTCGGCGAGG AGCGTGCGAC TGAGGCCGGC
    AGCTTGGAAG CCCGGCTCCG GGAGAGCGAG CAGGCCCGTG CCCTGCTGGA GCGGGAGGCC
    GAGGAGGCTC GCAGGCAGCT GGCCGCCTTG GGCCACACGG AACCCCCACT CGTGGCTGAG
    GCCCCCTGGG CCCGCCGGCC TCTGGATCCG CGGCGTCGGA GCCTCCCTGC TGGCGATGCC
    CTGTACTTGA GTTTCACACC CCCACAGCCC AGCCGAGGCC ACGACCGCCT GGATTTGCCT
    GTAACTATTC GCTCTGTCCA TCGACCCTTT GAAGACCGAG AGAGGCAGGA GCTGGGCAGC
    CCCGACGAGC GGCTGCAAGA TAGCAGTGAC CCTGACACCG GCAGCGAGGA GGAGGGTAGC
    AGCCGTCTGT CTCCACCTCA TAGTCCACGA GATTTCACCC GAATGCAGGA CATCCCGGAA
    GAGACTGAGA GCCGAGATGG GGAGCCTGTA GCTTCTGAGA GCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.