WRN gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_024986203.1	XP_024841971.1	Werner syndrome ATP-dependent helicase isoform X2
XM_024986205.1	XP_024841973.1	Werner syndrome ATP-dependent helicase isoform X3
NM_001143857.3	NP_001137329.3	Werner syndrome ATP-dependent helicase
XM_024986204.1	XP_024841972.1	Werner syndrome ATP-dependent helicase isoform X3
XM_024986207.1	XP_024841975.1	Werner syndrome ATP-dependent helicase isoform X5
XM_024986208.1	XP_024841976.1	Werner syndrome ATP-dependent helicase isoform X6
NM_001143857.2	NP_001137329.2	Werner syndrome ATP-dependent helicase
XM_024986202.1	XP_024841970.1	Werner syndrome ATP-dependent helicase isoform X1
XM_024986206.1	XP_024841974.1	Werner syndrome ATP-dependent helicase isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa239044	NM_001143857.3 Latest version!	Bos taurus WRN RecQ like helicase (WRN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231316	XM_024986202.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231317	XM_024986203.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231318	XM_024986204.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231318	XM_024986205.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231319	XM_024986206.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231320	XM_024986207.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa231321	XM_024986208.1 Latest version!	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa39357	NM_001143857.2 Latest version!	Bos taurus Werner syndrome, RecQ helicase-like (WRN), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

WRN ( NM_001143857.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137329.3 Bos taurus WRN RecQ like helicase (WRN), mRNA.

Price & Availability↑

CloneID	OBa239044
Clone ID Related Accession (Same CDS sequence)	NM_001143857.3
Accession Version	NM_001143857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DY106259.1, NKLS02000027.1, BI680560.1, GH316016.1, BF075592.1, EE354110.1, DN532432.1, DN531055.1, CK976011.1, DY137570.1 and CK975716.1. On May 30, 2018 this sequence version replaced NM_001143857.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145424 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137329.3
CDS	158..4372
Translation	MSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTETKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	NM_001143857.3
Organism	Bos taurus(cattle)
Definition	Bos taurus WRN RecQ like helicase (WRN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143857.3

ORF Insert Sequence:

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986202.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841970.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa231316
Clone ID Related Accession (Same CDS sequence)	XM_024986202.1
Accession Version	XM_024986202.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG 
TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC 
ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG 
CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT 
ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA 
ATGAGACCTA CTACAGTTGA AAATGTGAAA AGGATCGATG GTGTTTCTGA AGGCAAAGCT 
GTGATGTTGG CCCCTCTGTT GGAAGTCATC AGACGTTTCT GTCAAATAAA TAGCGTTCAG 
ACAGACCTCT TTTCAAGCTC AGAACTACAG GAAGAAGAGA AGAAAAATTT GGTGCTGGAA 
AATAAAGCAA ATTCACTTTC ACCGTCTATG GCCATTACAT ATTTTTTTTT CCAAGAAAAG 
AAAATGTCTT TGAAGGATAC AGCTGAGAAG AGGACTCTGC CTCTCACAGC AGTTGGCATG 
CACTTATCCC AGGCGATGAA AGCTGGCTAC CCAGTCGATA CAGAGCGAGC AGGCCTGACT 
CCAGAGATTC AAAAGATTAT TGCTGATGTT ATCCGAAACC CTCCCATCAA CTCAGATACA 
AATAAAATTA ACCTAATTAG AATATATGTT CCTGAAAACA TTGACACGTA TCTTATCCAC 
ATGACAATCA AGATCCTGGA AAAAGATGGT GACCACAGAA TGCGATGCCA CTCTTCATGT 
GATCCCAACA AAAAGCGACG TTTCCCCGAC CCTGAAGAGA GCTCTTCAAA TTCTAAGAGA 
ACCAAGGAAG AAGTAGACAC TGAGGCCAAG ACTTCGTCTG AAATGTCCAA GAGAAAATTA 
CCTTGGTGGT TTGCCAAAGG AAATGACACC TTACCTGATA CCAGCAAGAA ATCAGTGGCC 
AAAACCAAAA ACAAGGGTCT TTTTAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841970.1
CDS	386..4615
Translation	MDLLKMSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986202.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986202.1

ORF Insert Sequence:

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG 
TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC 
ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG 
CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT 
ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA 
ATGAGACCTA CTACAGTTGA AAATGTGAAA AGGATCGATG GTGTTTCTGA AGGCAAAGCT 
GTGATGTTGG CCCCTCTGTT GGAAGTCATC AGACGTTTCT GTCAAATAAA TAGCGTTCAG 
ACAGACCTCT TTTCAAGCTC AGAACTACAG GAAGAAGAGA AGAAAAATTT GGTGCTGGAA 
AATAAAGCAA ATTCACTTTC ACCGTCTATG GCCATTACAT ATTTTTTTTT CCAAGAAAAG 
AAAATGTCTT TGAAGGATAC AGCTGAGAAG AGGACTCTGC CTCTCACAGC AGTTGGCATG 
CACTTATCCC AGGCGATGAA AGCTGGCTAC CCAGTCGATA CAGAGCGAGC AGGCCTGACT 
CCAGAGATTC AAAAGATTAT TGCTGATGTT ATCCGAAACC CTCCCATCAA CTCAGATACA 
AATAAAATTA ACCTAATTAG AATATATGTT CCTGAAAACA TTGACACGTA TCTTATCCAC 
ATGACAATCA AGATCCTGGA AAAAGATGGT GACCACAGAA TGCGATGCCA CTCTTCATGT 
GATCCCAACA AAAAGCGACG TTTCCCCGAC CCTGAAGAGA GCTCTTCAAA TTCTAAGAGA 
ACCAAGGAAG AAGTAGACAC TGAGGCCAAG ACTTCGTCTG AAATGTCCAA GAGAAAATTA 
CCTTGGTGGT TTGCCAAAGG AAATGACACC TTACCTGATA CCAGCAAGAA ATCAGTGGCC 
AAAACCAAAA ACAAGGGTCT TTTTAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986203.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841971.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

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CloneID	OBa231317
Clone ID Related Accession (Same CDS sequence)	XM_024986203.1
Accession Version	XM_024986203.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGT TTAACTTGGA TCAGCTGTAT 
TCTTACAAAG TGCATGATAA AATTTCTTCT GGGAGTAACA GTCCTAAAAA CAGCATCATA 
GTGTCATCAC CAAGAAAATA TTACAAGTCC TCAGAACCTG TTATTTCCGC TCAAGAGCAG 
GAGACTCAGA CTGCATTATA TGGCGAATTG GTAGCAGCTA GGCAGAAACA CGCCAATATG 
ATGGATGTTC CTCCGGCTAT TCTGGCAACA AATAAGATAT TGTTGGATAT GGCCAAAATG 
AGACCTACTA CAGTTGAAAA TGTGAAAAGG ATCGATGGTG TTTCTGAAGG CAAAGCTGTG 
ATGTTGGCCC CTCTGTTGGA AGTCATCAGA CGTTTCTGTC AAATAAATAG CGTTCAGACA 
GACCTCTTTT CAAGCTCAGA ACTACAGGAA GAAGAGAAGA AAAATTTGGT GCTGGAAAAT 
AAAGCAAATT CACTTTCACC GTCTATGGCC ATTACATATT TTTTTTTCCA AGAAAAGAAA 
ATGTCTTTGA AGGATACAGC TGAGAAGAGG ACTCTGCCTC TCACAGCAGT TGGCATGCAC 
TTATCCCAGG CGATGAAAGC TGGCTACCCA GTCGATACAG AGCGAGCAGG CCTGACTCCA 
GAGATTCAAA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATACAAAT 
AAAATTAACC TAATTAGAAT ATATGTTCCT GAAAACATTG ACACGTATCT TATCCACATG 
ACAATCAAGA TCCTGGAAAA AGATGGTGAC CACAGAATGC GATGCCACTC TTCATGTGAT 
CCCAACAAAA AGCGACGTTT CCCCGACCCT GAAGAGAGCT CTTCAAATTC TAAGAGAACC 
AAGGAAGAAG TAGACACTGA GGCCAAGACT TCGTCTGAAA TGTCCAAGAG AAAATTACCT 
TGGTGGTTTG CCAAAGGAAA TGACACCTTA CCTGATACCA GCAAGAAATC AGTGGCCAAA 
ACCAAAAACA AGGGTCTTTT TAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841971.1
CDS	386..4612
Translation	MDLLKMSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986203.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986203.1

ORF Insert Sequence:

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGT TTAACTTGGA TCAGCTGTAT 
TCTTACAAAG TGCATGATAA AATTTCTTCT GGGAGTAACA GTCCTAAAAA CAGCATCATA 
GTGTCATCAC CAAGAAAATA TTACAAGTCC TCAGAACCTG TTATTTCCGC TCAAGAGCAG 
GAGACTCAGA CTGCATTATA TGGCGAATTG GTAGCAGCTA GGCAGAAACA CGCCAATATG 
ATGGATGTTC CTCCGGCTAT TCTGGCAACA AATAAGATAT TGTTGGATAT GGCCAAAATG 
AGACCTACTA CAGTTGAAAA TGTGAAAAGG ATCGATGGTG TTTCTGAAGG CAAAGCTGTG 
ATGTTGGCCC CTCTGTTGGA AGTCATCAGA CGTTTCTGTC AAATAAATAG CGTTCAGACA 
GACCTCTTTT CAAGCTCAGA ACTACAGGAA GAAGAGAAGA AAAATTTGGT GCTGGAAAAT 
AAAGCAAATT CACTTTCACC GTCTATGGCC ATTACATATT TTTTTTTCCA AGAAAAGAAA 
ATGTCTTTGA AGGATACAGC TGAGAAGAGG ACTCTGCCTC TCACAGCAGT TGGCATGCAC 
TTATCCCAGG CGATGAAAGC TGGCTACCCA GTCGATACAG AGCGAGCAGG CCTGACTCCA 
GAGATTCAAA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATACAAAT 
AAAATTAACC TAATTAGAAT ATATGTTCCT GAAAACATTG ACACGTATCT TATCCACATG 
ACAATCAAGA TCCTGGAAAA AGATGGTGAC CACAGAATGC GATGCCACTC TTCATGTGAT 
CCCAACAAAA AGCGACGTTT CCCCGACCCT GAAGAGAGCT CTTCAAATTC TAAGAGAACC 
AAGGAAGAAG TAGACACTGA GGCCAAGACT TCGTCTGAAA TGTCCAAGAG AAAATTACCT 
TGGTGGTTTG CCAAAGGAAA TGACACCTTA CCTGATACCA GCAAGAAATC AGTGGCCAAA 
ACCAAAAACA AGGGTCTTTT TAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986205.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841973.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa231318
Clone ID Related Accession (Same CDS sequence)	XM_024986205.1 , XM_024986204.1
Accession Version	XM_024986205.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841973.1
CDS	180..4394
Translation	MSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986205.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986205.1

ORF Insert Sequence:

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986204.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841972.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa231318
Clone ID Related Accession (Same CDS sequence)	XM_024986205.1 , XM_024986204.1
Accession Version	XM_024986204.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841972.1
CDS	206..4420
Translation	MSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986204.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986204.1

ORF Insert Sequence:

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986206.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841974.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

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CloneID	OBa231319
Clone ID Related Accession (Same CDS sequence)	XM_024986206.1
Accession Version	XM_024986206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGTTTAAC TTGGATCAGC TGTATTCTTA CAAAGTGCAT 
GATAAAATTT CTTCTGGGAG TAACAGTCCT AAAAACAGCA TCATAGTGTC ATCACCAAGA 
AAATATTACA AGTCCTCAGA ACCTGTTATT TCCGCTCAAG AGCAGGAGAC TCAGACTGCA 
TTATATGGCG AATTGGTAGC AGCTAGGCAG AAACACGCCA ATATGATGGA TGTTCCTCCG 
GCTATTCTGG CAACAAATAA GATATTGTTG GATATGGCCA AAATGAGACC TACTACAGTT 
GAAAATGTGA AAAGGATCGA TGGTGTTTCT GAAGGCAAAG CTGTGATGTT GGCCCCTCTG 
TTGGAAGTCA TCAGACGTTT CTGTCAAATA AATAGCGTTC AGACAGACCT CTTTTCAAGC 
TCAGAACTAC AGGAAGAAGA GAAGAAAAAT TTGGTGCTGG AAAATAAAGC AAATTCACTT 
TCACCGTCTA TGGCCATTAC ATATTTTTTT TTCCAAGAAA AGAAAATGTC TTTGAAGGAT 
ACAGCTGAGA AGAGGACTCT GCCTCTCACA GCAGTTGGCA TGCACTTATC CCAGGCGATG 
AAAGCTGGCT ACCCAGTCGA TACAGAGCGA GCAGGCCTGA CTCCAGAGAT TCAAAAGATT 
ATTGCTGATG TTATCCGAAA CCCTCCCATC AACTCAGATA CAAATAAAAT TAACCTAATT 
AGAATATATG TTCCTGAAAA CATTGACACG TATCTTATCC ACATGACAAT CAAGATCCTG 
GAAAAAGATG GTGACCACAG AATGCGATGC CACTCTTCAT GTGATCCCAA CAAAAAGCGA 
CGTTTCCCCG ACCCTGAAGA GAGCTCTTCA AATTCTAAGA GAACCAAGGA AGAAGTAGAC 
ACTGAGGCCA AGACTTCGTC TGAAATGTCC AAGAGAAAAT TACCTTGGTG GTTTGCCAAA 
GGAAATGACA CCTTACCTGA TACCAGCAAG AAATCAGTGG CCAAAACCAA AAACAAGGGT 
CTTTTTAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841974.1
CDS	221..4432
Translation	MSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986206.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986206.1

ORF Insert Sequence:

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGTTTAAC TTGGATCAGC TGTATTCTTA CAAAGTGCAT 
GATAAAATTT CTTCTGGGAG TAACAGTCCT AAAAACAGCA TCATAGTGTC ATCACCAAGA 
AAATATTACA AGTCCTCAGA ACCTGTTATT TCCGCTCAAG AGCAGGAGAC TCAGACTGCA 
TTATATGGCG AATTGGTAGC AGCTAGGCAG AAACACGCCA ATATGATGGA TGTTCCTCCG 
GCTATTCTGG CAACAAATAA GATATTGTTG GATATGGCCA AAATGAGACC TACTACAGTT 
GAAAATGTGA AAAGGATCGA TGGTGTTTCT GAAGGCAAAG CTGTGATGTT GGCCCCTCTG 
TTGGAAGTCA TCAGACGTTT CTGTCAAATA AATAGCGTTC AGACAGACCT CTTTTCAAGC 
TCAGAACTAC AGGAAGAAGA GAAGAAAAAT TTGGTGCTGG AAAATAAAGC AAATTCACTT 
TCACCGTCTA TGGCCATTAC ATATTTTTTT TTCCAAGAAA AGAAAATGTC TTTGAAGGAT 
ACAGCTGAGA AGAGGACTCT GCCTCTCACA GCAGTTGGCA TGCACTTATC CCAGGCGATG 
AAAGCTGGCT ACCCAGTCGA TACAGAGCGA GCAGGCCTGA CTCCAGAGAT TCAAAAGATT 
ATTGCTGATG TTATCCGAAA CCCTCCCATC AACTCAGATA CAAATAAAAT TAACCTAATT 
AGAATATATG TTCCTGAAAA CATTGACACG TATCTTATCC ACATGACAAT CAAGATCCTG 
GAAAAAGATG GTGACCACAG AATGCGATGC CACTCTTCAT GTGATCCCAA CAAAAAGCGA 
CGTTTCCCCG ACCCTGAAGA GAGCTCTTCA AATTCTAAGA GAACCAAGGA AGAAGTAGAC 
ACTGAGGCCA AGACTTCGTC TGAAATGTCC AAGAGAAAAT TACCTTGGTG GTTTGCCAAA 
GGAAATGACA CCTTACCTGA TACCAGCAAG AAATCAGTGG CCAAAACCAA AAACAAGGGT 
CTTTTTAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986207.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841975.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

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CloneID	OBa231320
Clone ID Related Accession (Same CDS sequence)	XM_024986207.1
Accession Version	XM_024986207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAT TGGATCACTG CTTTTCCCTT GAAGACTCAG AAGATACACA GGACTTTGGT 
CCGCAAGCAT TCCAGTTCTT ATCTGCTGTG ACCATCTTAG GAGGAAGGTT CGGCATTGGG 
ATTCCAATTT TATTTCTCCG AGGATCTAAT TCCCAGCGTC TTGAAGATAG ATACCGCAGC 
CACAGTGTCT TTGGTACGGG CAAGGACCAC ACAGAGAAAT GGTGGAAGGC TCTTTCCCGT 
CAGCTCATCA TTGAGGGATT CTTGGCAGAA GTTCCTGGGC ACAGCAAATA CGTAAAGATT 
TGCACCCTTA CGGAAAAGGG TAGAAACTGG TTTGCTAAAG CCAAAACAGA ATCTCCTCCG 
AGGCTTTTCC TTCAAACCAA TGAACAATTG TGTCCAAGGA CATTTCTACC AAGTTCTAAA 
GCTGTATCTT CAGGCACTGA GGAACATGTC TCTGATCAAG TACCAGTTAA ATTAACTTCA 
GAGAAGCAGT TTAACTTGGA TCAGCTGTAT TCTTACAAAG TGCATGATAA AATTTCTTCT 
GGGAGTAACA GTCCTAAAAA CAGCATCATA GTGTCATCAC CAAGAAAATA TTACAAGTCC 
TCAGAACCTG TTATTTCCGC TCAAGAGCAG GAGACTCAGA CTGCATTATA TGGCGAATTG 
GTAGCAGCTA GGCAGAAACA CGCCAATATG ATGGATGTTC CTCCGGCTAT TCTGGCAACA 
AATAAGATAT TGTTGGATAT GGCCAAAATG AGACCTACTA CAGTTGAAAA TGTGAAAAGG 
ATCGATGGTG TTTCTGAAGG CAAAGCTGTG ATGTTGGCCC CTCTGTTGGA AGTCATCAGA 
CGTTTCTGTC AAATAAATAG CGTTCAGACA GACCTCTTTT CAAGCTCAGA ACTACAGGAA 
GAAGAGAAGA AAAATTTGGT GCTGGAAAAT AAAGCAAATT CACTTTCACC GTCTATGGCC 
ATTACATATT TTTTTTTCCA AGAAAAGAAA ATGTCTTTGA AGGATACAGC TGAGAAGAGG 
ACTCTGCCTC TCACAGCAGT TGGCATGCAC TTATCCCAGG CGATGAAAGC TGGCTACCCA 
GTCGATACAG AGCGAGCAGG CCTGACTCCA GAGATTCAAA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATACAAAT AAAATTAACC TAATTAGAAT ATATGTTCCT 
GAAAACATTG ACACGTATCT TATCCACATG ACAATCAAGA TCCTGGAAAA AGATGGTGAC 
CACAGAATGC GATGCCACTC TTCATGTGAT CCCAACAAAA AGCGACGTTT CCCCGACCCT 
GAAGAGAGCT CTTCAAATTC TAAGAGAACC AAGGAAGAAG TAGACACTGA GGCCAAGACT 
TCGTCTGAAA TGTCCAAGAG AAAATTACCT TGGTGGTTTG CCAAAGGAAA TGACACCTTA 
CCTGATACCA GCAAGAAATC AGTGGCCAAA ACCAAAAACA AGGGTCTTTT TAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841975.1
CDS	386..4522
Translation	MDLLKMSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRRFPDPEESSSNSKRTKEEVDTEAKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	XM_024986207.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986207.1

ORF Insert Sequence:

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAT TGGATCACTG CTTTTCCCTT GAAGACTCAG AAGATACACA GGACTTTGGT 
CCGCAAGCAT TCCAGTTCTT ATCTGCTGTG ACCATCTTAG GAGGAAGGTT CGGCATTGGG 
ATTCCAATTT TATTTCTCCG AGGATCTAAT TCCCAGCGTC TTGAAGATAG ATACCGCAGC 
CACAGTGTCT TTGGTACGGG CAAGGACCAC ACAGAGAAAT GGTGGAAGGC TCTTTCCCGT 
CAGCTCATCA TTGAGGGATT CTTGGCAGAA GTTCCTGGGC ACAGCAAATA CGTAAAGATT 
TGCACCCTTA CGGAAAAGGG TAGAAACTGG TTTGCTAAAG CCAAAACAGA ATCTCCTCCG 
AGGCTTTTCC TTCAAACCAA TGAACAATTG TGTCCAAGGA CATTTCTACC AAGTTCTAAA 
GCTGTATCTT CAGGCACTGA GGAACATGTC TCTGATCAAG TACCAGTTAA ATTAACTTCA 
GAGAAGCAGT TTAACTTGGA TCAGCTGTAT TCTTACAAAG TGCATGATAA AATTTCTTCT 
GGGAGTAACA GTCCTAAAAA CAGCATCATA GTGTCATCAC CAAGAAAATA TTACAAGTCC 
TCAGAACCTG TTATTTCCGC TCAAGAGCAG GAGACTCAGA CTGCATTATA TGGCGAATTG 
GTAGCAGCTA GGCAGAAACA CGCCAATATG ATGGATGTTC CTCCGGCTAT TCTGGCAACA 
AATAAGATAT TGTTGGATAT GGCCAAAATG AGACCTACTA CAGTTGAAAA TGTGAAAAGG 
ATCGATGGTG TTTCTGAAGG CAAAGCTGTG ATGTTGGCCC CTCTGTTGGA AGTCATCAGA 
CGTTTCTGTC AAATAAATAG CGTTCAGACA GACCTCTTTT CAAGCTCAGA ACTACAGGAA 
GAAGAGAAGA AAAATTTGGT GCTGGAAAAT AAAGCAAATT CACTTTCACC GTCTATGGCC 
ATTACATATT TTTTTTTCCA AGAAAAGAAA ATGTCTTTGA AGGATACAGC TGAGAAGAGG 
ACTCTGCCTC TCACAGCAGT TGGCATGCAC TTATCCCAGG CGATGAAAGC TGGCTACCCA 
GTCGATACAG AGCGAGCAGG CCTGACTCCA GAGATTCAAA AGATTATTGC TGATGTTATC 
CGAAACCCTC CCATCAACTC AGATACAAAT AAAATTAACC TAATTAGAAT ATATGTTCCT 
GAAAACATTG ACACGTATCT TATCCACATG ACAATCAAGA TCCTGGAAAA AGATGGTGAC 
CACAGAATGC GATGCCACTC TTCATGTGAT CCCAACAAAA AGCGACGTTT CCCCGACCCT 
GAAGAGAGCT CTTCAAATTC TAAGAGAACC AAGGAAGAAG TAGACACTGA GGCCAAGACT 
TCGTCTGAAA TGTCCAAGAG AAAATTACCT TGGTGGTTTG CCAAAGGAAA TGACACCTTA 
CCTGATACCA GCAAGAAATC AGTGGCCAAA ACCAAAAACA AGGGTCTTTT TAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( XM_024986208.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024841976.1 Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

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CloneID	OBa231321
Clone ID Related Accession (Same CDS sequence)	XM_024986208.1
Accession Version	XM_024986208.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG 
TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC 
ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG 
CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT 
ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA 
ATGAGACAGA CCTCTTTTCA AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC 
TGGAAAATAA AGCAAATTCA CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG 
AAAAGAAAAT GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024841976.1
CDS	388..4005
Translation	MDLLKMSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDHTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRQTSFQAQNYRKKRRKIWCWKIKQIHFHRLWPLHIFFSKKRKCL

Target ORF information:

RefSeq Version	XM_024986208.1
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986208.1

ORF Insert Sequence:

ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG 
AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT 
GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT 
GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC 
CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA 
GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG 
GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT 
GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT 
GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC 
GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT 
GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC 
AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC 
AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA 
CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG 
ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG 
GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT 
GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA 
AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG 
AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA 
CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT 
CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT 
GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT 
CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT 
GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA 
CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT 
AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT 
GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT 
GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT 
GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA 
GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC 
TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG 
GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC 
CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA 
GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT 
TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT 
CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC 
ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG 
TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT 
AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT 
AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT 
TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG 
GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT 
CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT 
AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA 
ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC 
CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT 
GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT 
AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC 
CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA 
GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC 
TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA 
TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT 
GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG 
TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC 
ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG 
CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT 
ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA 
ATGAGACAGA CCTCTTTTCA AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC 
TGGAAAATAA AGCAAATTCA CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG 
AAAAGAAAAT GTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

WRN ( NM_001143857.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137329.2 Bos taurus Werner syndrome, RecQ helicase-like (WRN), mRNA.

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CloneID	OBa39357
Clone ID Related Accession (Same CDS sequence)	NM_001143857.2
Accession Version	NM_001143857.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-22
Organism	Bos taurus(cattle)
Product	Werner syndrome ATP-dependent helicase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DY106259.1, AAFC03085829.1, AAFC03085827.1, BI680560.1, GH316016.1, BF075592.1, AAFC03085828.1, AAFC03085830.1, EE354110.1, DN532432.1, DN531055.1, CK976011.1, DY137570.1, CK975716.1 and AAFC02040296.1. On Jun 2, 2010 this sequence version replaced NM_001143857.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCGCAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGATGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001137329.2
CDS	158..4372
Translation	MSEQTLERGGQRKLPDWMSVQKSYTVEEKKASVRKSVLEDHLPFLEFTGSTVYSYEANDCSLLSEDISMSLSDGDVVGFDIEWPPFKKGTLSRVALIQLCVAESKCYLFHISSMSGFPQGLKMLLENEAIKKAGVGIEQDQWKLLRDFDINLKSIVELTDVANEKLKCIETWSLNGLVKYLFGKQLLKDRSIRCSDWSRFPLTEDQKVYAATDAYAGFIIYQKLANLSDPVQIFVKSKEEEILPSDVKKQLTSISKEMMDLAERLPDTFGKLENPQRVSLLLKDISENLYSLRMMMFGSPEKELRPSDNFHLLPLEEPTAGEAQEKQIRAHNIFPEVEDEAWDATPDGLIEHDGEKVLGNQMKQEDGFEDELEGKKLEENTERNCLMSLDVTEEELQLWEQQAEEKHLTDVTCTSPEHSSSKDNEEDLSYIIESDEDLEMEMLKSLENLKSGMVDPNHSKCIEMEKNLDLPSEEDDEDENEAVEEEKETDEDCLLPRPTEKQINCLKTYFGHSSFKPVQWKVIHSVLEERRDNVVVMTTGYGKSLCFQYPPVYAGGIGLVISPLISLMEDQVLQLEISSIPACFLGSAQSKNVVEDIKLGKYRIVYLTPEFCSGNLELLRQLEANIGITLIAVDEAHCISEWGHDFRNSFRDLGCLKAEFPKVPIVALTATAGSSVREDIVCCLNLKDPQITCTGFDRPNLYLEVGRKTGDILQDLKQFLVCKSSSAWEFEGPTIIYCPSRKMTEQVTAELKKLKLACGTYHAGLSINLRKQVHHRFMRDEIQCVIATVAFGMGINKADIRQVIHYGAPKEMESYYQEIGRAGRDGLQSSCHVLWAPADMNLNRCLLSEIRNEKFRLYKLKMMGKMEKYLYSSKCRRQIILSHFEDKQLRKASVGIMGTENCCDNCKSRLDHCFSLEDSEDTQDFGPQAFQFLSAVTILGGRFGIGIPILFLRGSNSQRLEDRYRSHSVFGTGKDRTEKWWKALSRQLIIEGFLAEVPGHSKYVKICTLTEKGRNWFAKAKTESPPRLFLQTNEQLCPRTFLPSSKAVSSGTEEHVSDQVPVKLTSEKQFNLDQLYSYKVHDKISSGSNSPKNSIIVSSPRKYYKSSEPVISAQEQETQTALYGELVAARQKHANMMDVPPAILATNKILLDMAKMRPTTVENVKRIDGVSEGKAVMLAPLLEVIRRFCQINSVQTDLFSSSELQEEEKKNLVLENKANSLSPSMAITYFFFQEKKMSLKDTAEKRTLPLTAVGMHLSQAMKAGYPVDTERAGLTPEIQKIIADVIRNPPINSDTNKINLIRIYVPENIDTYLIHMTIKILEKDGDHRMRCHSSCDPNKKRCFPDPEESSSNSKRTKEEVDTETKTSSEMSKRKLPWWFAKGNDTLPDTSKKSVAKTKNKGLFS

Target ORF information:

RefSeq Version	NM_001143857.2
Organism	Bos taurus(cattle)
Definition	Bos taurus Werner syndrome, RecQ helicase-like (WRN), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001143857.2

ORF Insert Sequence:

ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT 
CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT 
TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC 
ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT 
GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA 
CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT 
CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT 
GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC 
CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT 
CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT 
TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG 
GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT 
CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA 
CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT 
GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT 
GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG 
GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT 
CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT 
ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA 
CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT 
TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG 
GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA 
TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC 
GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA 
AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG 
AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG 
TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC 
ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT 
CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC 
AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG 
CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT 
GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC 
CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT 
GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC 
TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT 
GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC 
AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA 
TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT 
GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT 
CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT 
AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG 
AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA 
AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC 
TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA 
AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA 
GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA 
GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT 
GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCGCAC AGAGAAATGG 
TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC 
AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC 
AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA 
TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA 
CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG 
CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA 
AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT 
GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT 
CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA 
GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT 
CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA 
AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA 
CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG 
GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG 
ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG 
ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA 
ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC 
CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG 
CGATGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA 
GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC 
AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG 
GGTCTTTTTA GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	WRN
Entrez Gene ID	505061
Full Name	Werner syndrome RecQ like helicase
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	WRN	NP_000544.2
Mus musculus (house mouse)	Wrn	NP_035851.3
Bos taurus (cattle)	WRN	NP_001137329.2
Caenorhabditis elegans (roundworm)	wrn-1	NP_495324.2
Canis lupus familiaris (dog)	WRN	XP_539984.4
Gallus gallus (chicken)	WRN	XP_001235033.2
Xenopus tropicalis (tropical clawed frog)	wrn	XP_002939304.1
Pan troglodytes (chimpanzee)	WRN	XP_528104.2
	WRN	XP_001085031.1
Rattus norvegicus (Norway rat)	RGD1564788	XP_006253317.1
Danio rerio (zebrafish)	wrn	XP_697980.5
Oryza sativa (rice)	Os07g0681600	NP_001060655.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

WRN cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

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