WRN cDNA ORF clone, Bos taurus(cattle)

The following WRN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the WRN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa239044 NM_001143857.3
Latest version!
Bos taurus WRN RecQ like helicase (WRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231316 XM_024986202.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231317 XM_024986203.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231318 XM_024986204.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231318 XM_024986205.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231319 XM_024986206.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231320 XM_024986207.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa231321 XM_024986208.1
Latest version!
Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa39357 NM_001143857.2
Latest version!
Bos taurus Werner syndrome, RecQ helicase-like (WRN), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa239044
    Clone ID Related Accession (Same CDS sequence) NM_001143857.3
    Accession Version NM_001143857.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-24
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DY106259.1, NKLS02000027.1, BI680560.1, GH316016.1, BF075592.1, EE354110.1, DN532432.1, DN531055.1, CK976011.1, DY137570.1 and CK975716.1. On May 30, 2018 this sequence version replaced NM_001143857.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145424 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137329.3
    CDS158..4372
    Translation

    Target ORF information:

    RefSeq Version NM_001143857.3
    Organism Bos taurus(cattle)
    Definition Bos taurus WRN RecQ like helicase (WRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143857.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231316
    Clone ID Related Accession (Same CDS sequence) XM_024986202.1
    Accession Version XM_024986202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4230bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG
    TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC
    ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG
    CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT
    ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA
    ATGAGACCTA CTACAGTTGA AAATGTGAAA AGGATCGATG GTGTTTCTGA AGGCAAAGCT
    GTGATGTTGG CCCCTCTGTT GGAAGTCATC AGACGTTTCT GTCAAATAAA TAGCGTTCAG
    ACAGACCTCT TTTCAAGCTC AGAACTACAG GAAGAAGAGA AGAAAAATTT GGTGCTGGAA
    AATAAAGCAA ATTCACTTTC ACCGTCTATG GCCATTACAT ATTTTTTTTT CCAAGAAAAG
    AAAATGTCTT TGAAGGATAC AGCTGAGAAG AGGACTCTGC CTCTCACAGC AGTTGGCATG
    CACTTATCCC AGGCGATGAA AGCTGGCTAC CCAGTCGATA CAGAGCGAGC AGGCCTGACT
    CCAGAGATTC AAAAGATTAT TGCTGATGTT ATCCGAAACC CTCCCATCAA CTCAGATACA
    AATAAAATTA ACCTAATTAG AATATATGTT CCTGAAAACA TTGACACGTA TCTTATCCAC
    ATGACAATCA AGATCCTGGA AAAAGATGGT GACCACAGAA TGCGATGCCA CTCTTCATGT
    GATCCCAACA AAAAGCGACG TTTCCCCGAC CCTGAAGAGA GCTCTTCAAA TTCTAAGAGA
    ACCAAGGAAG AAGTAGACAC TGAGGCCAAG ACTTCGTCTG AAATGTCCAA GAGAAAATTA
    CCTTGGTGGT TTGCCAAAGG AAATGACACC TTACCTGATA CCAGCAAGAA ATCAGTGGCC
    AAAACCAAAA ACAAGGGTCT TTTTAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841970.1
    CDS386..4615
    Translation

    Target ORF information:

    RefSeq Version XM_024986202.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG
    TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC
    ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG
    CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT
    ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA
    ATGAGACCTA CTACAGTTGA AAATGTGAAA AGGATCGATG GTGTTTCTGA AGGCAAAGCT
    GTGATGTTGG CCCCTCTGTT GGAAGTCATC AGACGTTTCT GTCAAATAAA TAGCGTTCAG
    ACAGACCTCT TTTCAAGCTC AGAACTACAG GAAGAAGAGA AGAAAAATTT GGTGCTGGAA
    AATAAAGCAA ATTCACTTTC ACCGTCTATG GCCATTACAT ATTTTTTTTT CCAAGAAAAG
    AAAATGTCTT TGAAGGATAC AGCTGAGAAG AGGACTCTGC CTCTCACAGC AGTTGGCATG
    CACTTATCCC AGGCGATGAA AGCTGGCTAC CCAGTCGATA CAGAGCGAGC AGGCCTGACT
    CCAGAGATTC AAAAGATTAT TGCTGATGTT ATCCGAAACC CTCCCATCAA CTCAGATACA
    AATAAAATTA ACCTAATTAG AATATATGTT CCTGAAAACA TTGACACGTA TCTTATCCAC
    ATGACAATCA AGATCCTGGA AAAAGATGGT GACCACAGAA TGCGATGCCA CTCTTCATGT
    GATCCCAACA AAAAGCGACG TTTCCCCGAC CCTGAAGAGA GCTCTTCAAA TTCTAAGAGA
    ACCAAGGAAG AAGTAGACAC TGAGGCCAAG ACTTCGTCTG AAATGTCCAA GAGAAAATTA
    CCTTGGTGGT TTGCCAAAGG AAATGACACC TTACCTGATA CCAGCAAGAA ATCAGTGGCC
    AAAACCAAAA ACAAGGGTCT TTTTAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231317
    Clone ID Related Accession (Same CDS sequence) XM_024986203.1
    Accession Version XM_024986203.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGT TTAACTTGGA TCAGCTGTAT
    TCTTACAAAG TGCATGATAA AATTTCTTCT GGGAGTAACA GTCCTAAAAA CAGCATCATA
    GTGTCATCAC CAAGAAAATA TTACAAGTCC TCAGAACCTG TTATTTCCGC TCAAGAGCAG
    GAGACTCAGA CTGCATTATA TGGCGAATTG GTAGCAGCTA GGCAGAAACA CGCCAATATG
    ATGGATGTTC CTCCGGCTAT TCTGGCAACA AATAAGATAT TGTTGGATAT GGCCAAAATG
    AGACCTACTA CAGTTGAAAA TGTGAAAAGG ATCGATGGTG TTTCTGAAGG CAAAGCTGTG
    ATGTTGGCCC CTCTGTTGGA AGTCATCAGA CGTTTCTGTC AAATAAATAG CGTTCAGACA
    GACCTCTTTT CAAGCTCAGA ACTACAGGAA GAAGAGAAGA AAAATTTGGT GCTGGAAAAT
    AAAGCAAATT CACTTTCACC GTCTATGGCC ATTACATATT TTTTTTTCCA AGAAAAGAAA
    ATGTCTTTGA AGGATACAGC TGAGAAGAGG ACTCTGCCTC TCACAGCAGT TGGCATGCAC
    TTATCCCAGG CGATGAAAGC TGGCTACCCA GTCGATACAG AGCGAGCAGG CCTGACTCCA
    GAGATTCAAA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATACAAAT
    AAAATTAACC TAATTAGAAT ATATGTTCCT GAAAACATTG ACACGTATCT TATCCACATG
    ACAATCAAGA TCCTGGAAAA AGATGGTGAC CACAGAATGC GATGCCACTC TTCATGTGAT
    CCCAACAAAA AGCGACGTTT CCCCGACCCT GAAGAGAGCT CTTCAAATTC TAAGAGAACC
    AAGGAAGAAG TAGACACTGA GGCCAAGACT TCGTCTGAAA TGTCCAAGAG AAAATTACCT
    TGGTGGTTTG CCAAAGGAAA TGACACCTTA CCTGATACCA GCAAGAAATC AGTGGCCAAA
    ACCAAAAACA AGGGTCTTTT TAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841971.1
    CDS386..4612
    Translation

    Target ORF information:

    RefSeq Version XM_024986203.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986203.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGT TTAACTTGGA TCAGCTGTAT
    TCTTACAAAG TGCATGATAA AATTTCTTCT GGGAGTAACA GTCCTAAAAA CAGCATCATA
    GTGTCATCAC CAAGAAAATA TTACAAGTCC TCAGAACCTG TTATTTCCGC TCAAGAGCAG
    GAGACTCAGA CTGCATTATA TGGCGAATTG GTAGCAGCTA GGCAGAAACA CGCCAATATG
    ATGGATGTTC CTCCGGCTAT TCTGGCAACA AATAAGATAT TGTTGGATAT GGCCAAAATG
    AGACCTACTA CAGTTGAAAA TGTGAAAAGG ATCGATGGTG TTTCTGAAGG CAAAGCTGTG
    ATGTTGGCCC CTCTGTTGGA AGTCATCAGA CGTTTCTGTC AAATAAATAG CGTTCAGACA
    GACCTCTTTT CAAGCTCAGA ACTACAGGAA GAAGAGAAGA AAAATTTGGT GCTGGAAAAT
    AAAGCAAATT CACTTTCACC GTCTATGGCC ATTACATATT TTTTTTTCCA AGAAAAGAAA
    ATGTCTTTGA AGGATACAGC TGAGAAGAGG ACTCTGCCTC TCACAGCAGT TGGCATGCAC
    TTATCCCAGG CGATGAAAGC TGGCTACCCA GTCGATACAG AGCGAGCAGG CCTGACTCCA
    GAGATTCAAA AGATTATTGC TGATGTTATC CGAAACCCTC CCATCAACTC AGATACAAAT
    AAAATTAACC TAATTAGAAT ATATGTTCCT GAAAACATTG ACACGTATCT TATCCACATG
    ACAATCAAGA TCCTGGAAAA AGATGGTGAC CACAGAATGC GATGCCACTC TTCATGTGAT
    CCCAACAAAA AGCGACGTTT CCCCGACCCT GAAGAGAGCT CTTCAAATTC TAAGAGAACC
    AAGGAAGAAG TAGACACTGA GGCCAAGACT TCGTCTGAAA TGTCCAAGAG AAAATTACCT
    TGGTGGTTTG CCAAAGGAAA TGACACCTTA CCTGATACCA GCAAGAAATC AGTGGCCAAA
    ACCAAAAACA AGGGTCTTTT TAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231318
    Clone ID Related Accession (Same CDS sequence) XM_024986205.1 , XM_024986204.1
    Accession Version XM_024986205.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841973.1
    CDS180..4394
    Translation

    Target ORF information:

    RefSeq Version XM_024986205.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986205.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231318
    Clone ID Related Accession (Same CDS sequence) XM_024986205.1 , XM_024986204.1
    Accession Version XM_024986204.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841972.1
    CDS206..4420
    Translation

    Target ORF information:

    RefSeq Version XM_024986204.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986204.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGACGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGG CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231319
    Clone ID Related Accession (Same CDS sequence) XM_024986206.1
    Accession Version XM_024986206.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4212bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGTTTAAC TTGGATCAGC TGTATTCTTA CAAAGTGCAT
    GATAAAATTT CTTCTGGGAG TAACAGTCCT AAAAACAGCA TCATAGTGTC ATCACCAAGA
    AAATATTACA AGTCCTCAGA ACCTGTTATT TCCGCTCAAG AGCAGGAGAC TCAGACTGCA
    TTATATGGCG AATTGGTAGC AGCTAGGCAG AAACACGCCA ATATGATGGA TGTTCCTCCG
    GCTATTCTGG CAACAAATAA GATATTGTTG GATATGGCCA AAATGAGACC TACTACAGTT
    GAAAATGTGA AAAGGATCGA TGGTGTTTCT GAAGGCAAAG CTGTGATGTT GGCCCCTCTG
    TTGGAAGTCA TCAGACGTTT CTGTCAAATA AATAGCGTTC AGACAGACCT CTTTTCAAGC
    TCAGAACTAC AGGAAGAAGA GAAGAAAAAT TTGGTGCTGG AAAATAAAGC AAATTCACTT
    TCACCGTCTA TGGCCATTAC ATATTTTTTT TTCCAAGAAA AGAAAATGTC TTTGAAGGAT
    ACAGCTGAGA AGAGGACTCT GCCTCTCACA GCAGTTGGCA TGCACTTATC CCAGGCGATG
    AAAGCTGGCT ACCCAGTCGA TACAGAGCGA GCAGGCCTGA CTCCAGAGAT TCAAAAGATT
    ATTGCTGATG TTATCCGAAA CCCTCCCATC AACTCAGATA CAAATAAAAT TAACCTAATT
    AGAATATATG TTCCTGAAAA CATTGACACG TATCTTATCC ACATGACAAT CAAGATCCTG
    GAAAAAGATG GTGACCACAG AATGCGATGC CACTCTTCAT GTGATCCCAA CAAAAAGCGA
    CGTTTCCCCG ACCCTGAAGA GAGCTCTTCA AATTCTAAGA GAACCAAGGA AGAAGTAGAC
    ACTGAGGCCA AGACTTCGTC TGAAATGTCC AAGAGAAAAT TACCTTGGTG GTTTGCCAAA
    GGAAATGACA CCTTACCTGA TACCAGCAAG AAATCAGTGG CCAAAACCAA AAACAAGGGT
    CTTTTTAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841974.1
    CDS221..4432
    Translation

    Target ORF information:

    RefSeq Version XM_024986206.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986206.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCACAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGTTTAAC TTGGATCAGC TGTATTCTTA CAAAGTGCAT
    GATAAAATTT CTTCTGGGAG TAACAGTCCT AAAAACAGCA TCATAGTGTC ATCACCAAGA
    AAATATTACA AGTCCTCAGA ACCTGTTATT TCCGCTCAAG AGCAGGAGAC TCAGACTGCA
    TTATATGGCG AATTGGTAGC AGCTAGGCAG AAACACGCCA ATATGATGGA TGTTCCTCCG
    GCTATTCTGG CAACAAATAA GATATTGTTG GATATGGCCA AAATGAGACC TACTACAGTT
    GAAAATGTGA AAAGGATCGA TGGTGTTTCT GAAGGCAAAG CTGTGATGTT GGCCCCTCTG
    TTGGAAGTCA TCAGACGTTT CTGTCAAATA AATAGCGTTC AGACAGACCT CTTTTCAAGC
    TCAGAACTAC AGGAAGAAGA GAAGAAAAAT TTGGTGCTGG AAAATAAAGC AAATTCACTT
    TCACCGTCTA TGGCCATTAC ATATTTTTTT TTCCAAGAAA AGAAAATGTC TTTGAAGGAT
    ACAGCTGAGA AGAGGACTCT GCCTCTCACA GCAGTTGGCA TGCACTTATC CCAGGCGATG
    AAAGCTGGCT ACCCAGTCGA TACAGAGCGA GCAGGCCTGA CTCCAGAGAT TCAAAAGATT
    ATTGCTGATG TTATCCGAAA CCCTCCCATC AACTCAGATA CAAATAAAAT TAACCTAATT
    AGAATATATG TTCCTGAAAA CATTGACACG TATCTTATCC ACATGACAAT CAAGATCCTG
    GAAAAAGATG GTGACCACAG AATGCGATGC CACTCTTCAT GTGATCCCAA CAAAAAGCGA
    CGTTTCCCCG ACCCTGAAGA GAGCTCTTCA AATTCTAAGA GAACCAAGGA AGAAGTAGAC
    ACTGAGGCCA AGACTTCGTC TGAAATGTCC AAGAGAAAAT TACCTTGGTG GTTTGCCAAA
    GGAAATGACA CCTTACCTGA TACCAGCAAG AAATCAGTGG CCAAAACCAA AAACAAGGGT
    CTTTTTAGTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231320
    Clone ID Related Accession (Same CDS sequence) XM_024986207.1
    Accession Version XM_024986207.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4137bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAT TGGATCACTG CTTTTCCCTT GAAGACTCAG AAGATACACA GGACTTTGGT
    CCGCAAGCAT TCCAGTTCTT ATCTGCTGTG ACCATCTTAG GAGGAAGGTT CGGCATTGGG
    ATTCCAATTT TATTTCTCCG AGGATCTAAT TCCCAGCGTC TTGAAGATAG ATACCGCAGC
    CACAGTGTCT TTGGTACGGG CAAGGACCAC ACAGAGAAAT GGTGGAAGGC TCTTTCCCGT
    CAGCTCATCA TTGAGGGATT CTTGGCAGAA GTTCCTGGGC ACAGCAAATA CGTAAAGATT
    TGCACCCTTA CGGAAAAGGG TAGAAACTGG TTTGCTAAAG CCAAAACAGA ATCTCCTCCG
    AGGCTTTTCC TTCAAACCAA TGAACAATTG TGTCCAAGGA CATTTCTACC AAGTTCTAAA
    GCTGTATCTT CAGGCACTGA GGAACATGTC TCTGATCAAG TACCAGTTAA ATTAACTTCA
    GAGAAGCAGT TTAACTTGGA TCAGCTGTAT TCTTACAAAG TGCATGATAA AATTTCTTCT
    GGGAGTAACA GTCCTAAAAA CAGCATCATA GTGTCATCAC CAAGAAAATA TTACAAGTCC
    TCAGAACCTG TTATTTCCGC TCAAGAGCAG GAGACTCAGA CTGCATTATA TGGCGAATTG
    GTAGCAGCTA GGCAGAAACA CGCCAATATG ATGGATGTTC CTCCGGCTAT TCTGGCAACA
    AATAAGATAT TGTTGGATAT GGCCAAAATG AGACCTACTA CAGTTGAAAA TGTGAAAAGG
    ATCGATGGTG TTTCTGAAGG CAAAGCTGTG ATGTTGGCCC CTCTGTTGGA AGTCATCAGA
    CGTTTCTGTC AAATAAATAG CGTTCAGACA GACCTCTTTT CAAGCTCAGA ACTACAGGAA
    GAAGAGAAGA AAAATTTGGT GCTGGAAAAT AAAGCAAATT CACTTTCACC GTCTATGGCC
    ATTACATATT TTTTTTTCCA AGAAAAGAAA ATGTCTTTGA AGGATACAGC TGAGAAGAGG
    ACTCTGCCTC TCACAGCAGT TGGCATGCAC TTATCCCAGG CGATGAAAGC TGGCTACCCA
    GTCGATACAG AGCGAGCAGG CCTGACTCCA GAGATTCAAA AGATTATTGC TGATGTTATC
    CGAAACCCTC CCATCAACTC AGATACAAAT AAAATTAACC TAATTAGAAT ATATGTTCCT
    GAAAACATTG ACACGTATCT TATCCACATG ACAATCAAGA TCCTGGAAAA AGATGGTGAC
    CACAGAATGC GATGCCACTC TTCATGTGAT CCCAACAAAA AGCGACGTTT CCCCGACCCT
    GAAGAGAGCT CTTCAAATTC TAAGAGAACC AAGGAAGAAG TAGACACTGA GGCCAAGACT
    TCGTCTGAAA TGTCCAAGAG AAAATTACCT TGGTGGTTTG CCAAAGGAAA TGACACCTTA
    CCTGATACCA GCAAGAAATC AGTGGCCAAA ACCAAAAACA AGGGTCTTTT TAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841975.1
    CDS386..4522
    Translation

    Target ORF information:

    RefSeq Version XM_024986207.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986207.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAT TGGATCACTG CTTTTCCCTT GAAGACTCAG AAGATACACA GGACTTTGGT
    CCGCAAGCAT TCCAGTTCTT ATCTGCTGTG ACCATCTTAG GAGGAAGGTT CGGCATTGGG
    ATTCCAATTT TATTTCTCCG AGGATCTAAT TCCCAGCGTC TTGAAGATAG ATACCGCAGC
    CACAGTGTCT TTGGTACGGG CAAGGACCAC ACAGAGAAAT GGTGGAAGGC TCTTTCCCGT
    CAGCTCATCA TTGAGGGATT CTTGGCAGAA GTTCCTGGGC ACAGCAAATA CGTAAAGATT
    TGCACCCTTA CGGAAAAGGG TAGAAACTGG TTTGCTAAAG CCAAAACAGA ATCTCCTCCG
    AGGCTTTTCC TTCAAACCAA TGAACAATTG TGTCCAAGGA CATTTCTACC AAGTTCTAAA
    GCTGTATCTT CAGGCACTGA GGAACATGTC TCTGATCAAG TACCAGTTAA ATTAACTTCA
    GAGAAGCAGT TTAACTTGGA TCAGCTGTAT TCTTACAAAG TGCATGATAA AATTTCTTCT
    GGGAGTAACA GTCCTAAAAA CAGCATCATA GTGTCATCAC CAAGAAAATA TTACAAGTCC
    TCAGAACCTG TTATTTCCGC TCAAGAGCAG GAGACTCAGA CTGCATTATA TGGCGAATTG
    GTAGCAGCTA GGCAGAAACA CGCCAATATG ATGGATGTTC CTCCGGCTAT TCTGGCAACA
    AATAAGATAT TGTTGGATAT GGCCAAAATG AGACCTACTA CAGTTGAAAA TGTGAAAAGG
    ATCGATGGTG TTTCTGAAGG CAAAGCTGTG ATGTTGGCCC CTCTGTTGGA AGTCATCAGA
    CGTTTCTGTC AAATAAATAG CGTTCAGACA GACCTCTTTT CAAGCTCAGA ACTACAGGAA
    GAAGAGAAGA AAAATTTGGT GCTGGAAAAT AAAGCAAATT CACTTTCACC GTCTATGGCC
    ATTACATATT TTTTTTTCCA AGAAAAGAAA ATGTCTTTGA AGGATACAGC TGAGAAGAGG
    ACTCTGCCTC TCACAGCAGT TGGCATGCAC TTATCCCAGG CGATGAAAGC TGGCTACCCA
    GTCGATACAG AGCGAGCAGG CCTGACTCCA GAGATTCAAA AGATTATTGC TGATGTTATC
    CGAAACCCTC CCATCAACTC AGATACAAAT AAAATTAACC TAATTAGAAT ATATGTTCCT
    GAAAACATTG ACACGTATCT TATCCACATG ACAATCAAGA TCCTGGAAAA AGATGGTGAC
    CACAGAATGC GATGCCACTC TTCATGTGAT CCCAACAAAA AGCGACGTTT CCCCGACCCT
    GAAGAGAGCT CTTCAAATTC TAAGAGAACC AAGGAAGAAG TAGACACTGA GGCCAAGACT
    TCGTCTGAAA TGTCCAAGAG AAAATTACCT TGGTGGTTTG CCAAAGGAAA TGACACCTTA
    CCTGATACCA GCAAGAAATC AGTGGCCAAA ACCAAAAACA AGGGTCTTTT TAGTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa231321
    Clone ID Related Accession (Same CDS sequence) XM_024986208.1
    Accession Version XM_024986208.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037354.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG
    TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC
    ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG
    CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT
    ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA
    ATGAGACAGA CCTCTTTTCA AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC
    TGGAAAATAA AGCAAATTCA CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG
    AAAAGAAAAT GTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024841976.1
    CDS388..4005
    Translation

    Target ORF information:

    RefSeq Version XM_024986208.1
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome RecQ like helicase (WRN), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024986208.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACCTTT TAAAGATGAG TGAACAAACG TTGGAAAGAG GAGGGCAGCG GAAACTTCCT 
    GATTGGATGT CTGTGCAGAA GAGCTATACA GTAGAAGAAA AAAAGGCATC TGTTCGGAAG
    AGTGTTTTGG AAGATCACCT GCCCTTCTTA GAATTCACTG GATCCACTGT GTACAGTTAT
    GAAGCTAATG ATTGTTCTTT GCTCTCAGAA GATATTAGCA TGAGTCTCTC TGATGGAGAT
    GTGGTCGGAT TTGACATCGA GTGGCCACCG TTCAAGAAAG GGACACTGAG CAGAGTGGCC
    CTCATTCAGC TGTGTGTGGC AGAGAGCAAG TGCTACTTGT TTCATATTTC TTCTATGTCA
    GGTTTCCCTC AGGGACTAAA AATGTTGCTT GAAAATGAAG CAATTAAAAA GGCAGGTGTG
    GGAATTGAAC AAGATCAGTG GAAACTTCTA CGTGACTTTG ATATCAACTT GAAGAGTATT
    GTGGAGCTGA CAGATGTTGC CAATGAAAAG CTGAAATGTA TAGAGACCTG GAGCCTCAAT
    GGTTTGGTTA AATACCTCTT TGGTAAACAG CTTCTGAAAG ACCGGTCTAT CCGCTGTAGC
    GACTGGAGTA GGTTTCCCCT CACTGAGGAC CAGAAAGTCT ATGCAGCCAC TGATGCTTAT
    GCTGGTTTCA TCATTTATCA AAAATTAGCA AATTTGAGTG ATCCTGTACA AATATTTGTC
    AAGAGTAAAG AGGAGGAAAT ACTACCTAGT GATGTGAAGA AACAATTGAC TTCAATTTCC
    AAGGAGATGA TGGATCTGGC TGAGCGTCTT CCTGATACTT TTGGAAAACT GGAAAATCCA
    CAGAGGGTTT CCCTACTATT GAAGGATATT TCAGAAAATC TGTATTCATT GAGGATGATG
    ATGTTTGGCT CCCCTGAGAA GGAACTGAGA CCCAGCGATA ACTTTCACTT ATTACCACTG
    GAAGAGCCAA CTGCTGGTGA AGCACAAGAG AAACAAATTA GAGCACATAA CATTTTTCCT
    GAAGTTGAAG ATGAGGCATG GGATGCAACA CCTGATGGTT TAATTGAACA TGATGGAGAA
    AAGGTACTTG GAAATCAAAT GAAACAAGAA GATGGATTTG AAGATGAACT GGAAGGCAAG
    AAGTTGGAAG AGAATACAGA AAGAAATTGT TTGATGTCAT TAGATGTTAC AGAAGAGGAA
    CTCCAACTTT GGGAACAACA GGCTGAGGAA AAGCATCTTA CTGATGTTAC TTGCACATCT
    CCTGAGCATT CATCTTCCAA GGATAATGAA GAGGATCTAT CTTACATAAT TGAGAGTGAT
    GAAGATTTAG AAATGGAGAT GCTTAAGTCT TTAGAAAACC TCAAAAGTGG CATGGTAGAT
    CCAAATCATT CAAAATGCAT AGAAATGGAA AAAAATCTAG ATCTTCCTTC TGAAGAAGAT
    GATGAAGATG AGAACGAAGC TGTTGAAGAA GAAAAAGAAA CTGATGAAGA CTGCCTGTTA
    CCAAGACCTA CTGAAAAGCA AATTAACTGC CTCAAGACCT ACTTTGGCCA TTCTAGTTTT
    AAGCCGGTTC AATGGAAAGT GATTCATTCT GTACTGGAAG AAAGAAGAGA TAATGTTGTT
    GTCATGACAA CTGGGTATGG AAAGAGTCTG TGCTTCCAGT ATCCACCTGT TTATGCAGGT
    GGGATTGGCC TTGTCATCTC TCCCCTTATT TCTTTGATGG AAGACCAAGT ACTTCAGCTT
    GAAATATCCA GTATTCCAGC TTGTTTTCTT GGATCAGCAC AGTCAAAAAA TGTTGTAGAA
    GATATTAAAT TAGGCAAATA CCGGATTGTA TACCTAACTC CAGAATTCTG TTCAGGTAAC
    TTGGAGCTGC TTCGGCAACT TGAGGCTAAT ATTGGTATCA CACTTATTGC TGTGGATGAG
    GCTCACTGTA TTTCTGAGTG GGGACATGAT TTTAGAAATT CATTCAGGGA TTTGGGTTGC
    CTAAAGGCAG AATTCCCGAA GGTTCCAATT GTTGCACTGA CTGCTACGGC AGGTTCTTCA
    GTCCGAGAAG ACATTGTATG TTGCTTAAAC CTGAAGGATC CTCAGATAAC CTGTACTGGT
    TTTGATCGAC CAAACTTGTA TTTAGAAGTT GGACGAAAAA CAGGAGACAT CCTCCAGGAT
    CTGAAGCAGT TTCTTGTCTG TAAGTCAAGT TCTGCTTGGG AATTTGAAGG TCCAACTATC
    ATCTATTGTC CTTCCAGAAA AATGACAGAG CAAGTTACAG CTGAACTTAA AAAACTGAAG
    TTAGCCTGTG GAACATACCA TGCAGGACTG AGTATTAACT TACGGAAGCA AGTTCATCAT
    AGGTTCATGA GAGATGAAAT TCAGTGTGTC ATAGCTACTG TAGCTTTTGG AATGGGCATT
    AATAAGGCTG ACATTCGCCA AGTCATTCAT TATGGTGCTC CTAAGGAAAT GGAATCCTAT
    TACCAGGAGA TTGGTAGAGC TGGCCGAGAT GGACTTCAAA GTTCTTGTCA CGTTCTTTGG
    GCTCCAGCAG ACATGAACCT AAATAGGTGC CTCCTTAGTG AGATACGCAA TGAAAAGTTT
    CGATTATACA AATTAAAGAT GATGGGAAAG ATGGAAAAAT ATCTTTATTC CAGCAAGTGT
    AGGCGACAAA TCATCTTGTC TCATTTTGAA GACAAACAAC TACGAAAGGC CTCCGTAGGA
    ATTATGGGAA CTGAAAACTG CTGTGATAAT TGCAAGTCCA GATTGGATCA CTGCTTTTCC
    CTTGAAGACT CAGAAGATAC ACAGGACTTT GGTCCGCAAG CATTCCAGTT CTTATCTGCT
    GTGACCATCT TAGGAGGAAG GTTCGGCATT GGGATTCCAA TTTTATTTCT CCGAGGATCT
    AATTCCCAGC GTCTTGAAGA TAGATACCGC AGCCACAGTG TCTTTGGTAC GGGCAAGGAC
    CACACAGAGA AATGGTGGAA GGCTCTTTCC CGTCAGCTCA TCATTGAGGG ATTCTTGGCA
    GAAGTTCCTG GGCACAGCAA ATACGTAAAG ATTTGCACCC TTACGGAAAA GGGTAGAAAC
    TGGTTTGCTA AAGCCAAAAC AGAATCTCCT CCGAGGCTTT TCCTTCAAAC CAATGAACAA
    TTGTGTCCAA GGACATTTCT ACCAAGTTCT AAAGCTGTAT CTTCAGGCAC TGAGGAACAT
    GTCTCTGATC AAGTACCAGT TAAATTAACT TCAGAGAAGC AGTTTAACTT GGATCAGCTG
    TATTCTTACA AAGTGCATGA TAAAATTTCT TCTGGGAGTA ACAGTCCTAA AAACAGCATC
    ATAGTGTCAT CACCAAGAAA ATATTACAAG TCCTCAGAAC CTGTTATTTC CGCTCAAGAG
    CAGGAGACTC AGACTGCATT ATATGGCGAA TTGGTAGCAG CTAGGCAGAA ACACGCCAAT
    ATGATGGATG TTCCTCCGGC TATTCTGGCA ACAAATAAGA TATTGTTGGA TATGGCCAAA
    ATGAGACAGA CCTCTTTTCA AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC
    TGGAAAATAA AGCAAATTCA CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG
    AAAAGAAAAT GTCTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa39357
    Clone ID Related Accession (Same CDS sequence) NM_001143857.2
    Accession Version NM_001143857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4215bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-22
    Organism Bos taurus(cattle)
    Product Werner syndrome ATP-dependent helicase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DY106259.1, AAFC03085829.1, AAFC03085827.1, BI680560.1, GH316016.1, BF075592.1, AAFC03085828.1, AAFC03085830.1, EE354110.1, DN532432.1, DN531055.1, CK976011.1, DY137570.1, CK975716.1 and AAFC02040296.1. On Jun 2, 2010 this sequence version replaced NM_001143857.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCGCAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGATGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137329.2
    CDS158..4372
    Translation

    Target ORF information:

    RefSeq Version NM_001143857.2
    Organism Bos taurus(cattle)
    Definition Bos taurus Werner syndrome, RecQ helicase-like (WRN), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001143857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGAGTGAAC AAACGTTGGA AAGAGGAGGG CAGCGGAAAC TTCCTGATTG GATGTCTGTG 
    CAGAAGAGCT ATACAGTAGA AGAAAAAAAG GCATCTGTTC GGAAGAGTGT TTTGGAAGAT
    CACCTGCCCT TCTTAGAATT CACTGGATCC ACTGTGTACA GTTATGAAGC TAATGATTGT
    TCTTTGCTCT CAGAAGATAT TAGCATGAGT CTCTCTGATG GAGATGTGGT CGGATTTGAC
    ATCGAGTGGC CACCGTTCAA GAAAGGGACA CTGAGCAGAG TGGCCCTCAT TCAGCTGTGT
    GTGGCAGAGA GCAAGTGCTA CTTGTTTCAT ATTTCTTCTA TGTCAGGTTT CCCTCAGGGA
    CTAAAAATGT TGCTTGAAAA TGAAGCAATT AAAAAGGCAG GTGTGGGAAT TGAACAAGAT
    CAGTGGAAAC TTCTACGTGA CTTTGATATC AACTTGAAGA GTATTGTGGA GCTGACAGAT
    GTTGCCAATG AAAAGCTGAA ATGTATAGAG ACCTGGAGCC TCAATGGTTT GGTTAAATAC
    CTCTTTGGTA AACAGCTTCT GAAAGACCGG TCTATCCGCT GTAGCGACTG GAGTAGGTTT
    CCCCTCACTG AGGACCAGAA AGTCTATGCA GCCACTGATG CTTATGCTGG TTTCATCATT
    TATCAAAAAT TAGCAAATTT GAGTGATCCT GTACAAATAT TTGTCAAGAG TAAAGAGGAG
    GAAATACTAC CTAGTGATGT GAAGAAACAA TTGACTTCAA TTTCCAAGGA GATGATGGAT
    CTGGCTGAGC GTCTTCCTGA TACTTTTGGA AAACTGGAAA ATCCACAGAG GGTTTCCCTA
    CTATTGAAGG ATATTTCAGA AAATCTGTAT TCATTGAGGA TGATGATGTT TGGCTCCCCT
    GAGAAGGAAC TGAGACCCAG CGATAACTTT CACTTATTAC CACTGGAAGA GCCAACTGCT
    GGTGAAGCAC AAGAGAAACA AATTAGAGCA CATAACATTT TTCCTGAAGT TGAAGATGAG
    GCATGGGATG CAACACCTGA TGGTTTAATT GAACATGATG GAGAAAAGGT ACTTGGAAAT
    CAAATGAAAC AAGAAGATGG ATTTGAAGAT GAACTGGAAG GCAAGAAGTT GGAAGAGAAT
    ACAGAAAGAA ATTGTTTGAT GTCATTAGAT GTTACAGAAG AGGAACTCCA ACTTTGGGAA
    CAACAGGCTG AGGAAAAGCA TCTTACTGAT GTTACTTGCA CATCTCCTGA GCATTCATCT
    TCCAAGGATA ATGAAGAGGA TCTATCTTAC ATAATTGAGA GTGATGAAGA TTTAGAAATG
    GAGATGCTTA AGTCTTTAGA AAACCTCAAA AGTGGCATGG TAGATCCAAA TCATTCAAAA
    TGCATAGAAA TGGAAAAAAA TCTAGATCTT CCTTCTGAAG AAGATGATGA AGATGAGAAC
    GAAGCTGTTG AAGAAGAAAA AGAAACTGAT GAAGACTGCC TGTTACCAAG ACCTACTGAA
    AAGCAAATTA ACTGCCTCAA GACCTACTTT GGCCATTCTA GTTTTAAGCC GGTTCAATGG
    AAAGTGATTC ATTCTGTACT GGAAGAAAGA AGAGATAATG TTGTTGTCAT GACAACTGGG
    TATGGAAAGA GTCTGTGCTT CCAGTATCCA CCTGTTTATG CAGGTGGGAT TGGCCTTGTC
    ATCTCTCCCC TTATTTCTTT GATGGAAGAC CAAGTACTTC AGCTTGAAAT ATCCAGTATT
    CCAGCTTGTT TTCTTGGATC AGCACAGTCA AAAAATGTTG TAGAAGATAT TAAATTAGGC
    AAATACCGGA TTGTATACCT AACTCCAGAA TTCTGTTCAG GTAACTTGGA GCTGCTTCGG
    CAACTTGAGG CTAATATTGG TATCACACTT ATTGCTGTGG ATGAGGCTCA CTGTATTTCT
    GAGTGGGGAC ATGATTTTAG AAATTCATTC AGGGATTTGG GTTGCCTAAA GGCAGAATTC
    CCGAAGGTTC CAATTGTTGC ACTGACTGCT ACGGCAGGTT CTTCAGTCCG AGAAGACATT
    GTATGTTGCT TAAACCTGAA GGATCCTCAG ATAACCTGTA CTGGTTTTGA TCGACCAAAC
    TTGTATTTAG AAGTTGGACG AAAAACAGGA GACATCCTCC AGGATCTGAA GCAGTTTCTT
    GTCTGTAAGT CAAGTTCTGC TTGGGAATTT GAAGGTCCAA CTATCATCTA TTGTCCTTCC
    AGAAAAATGA CAGAGCAAGT TACAGCTGAA CTTAAAAAAC TGAAGTTAGC CTGTGGAACA
    TACCATGCAG GACTGAGTAT TAACTTACGG AAGCAAGTTC ATCATAGGTT CATGAGAGAT
    GAAATTCAGT GTGTCATAGC TACTGTAGCT TTTGGAATGG GCATTAATAA GGCTGACATT
    CGCCAAGTCA TTCATTATGG TGCTCCTAAG GAAATGGAAT CCTATTACCA GGAGATTGGT
    AGAGCTGGCC GAGATGGACT TCAAAGTTCT TGTCACGTTC TTTGGGCTCC AGCAGACATG
    AACCTAAATA GGTGCCTCCT TAGTGAGATA CGCAATGAAA AGTTTCGATT ATACAAATTA
    AAGATGATGG GAAAGATGGA AAAATATCTT TATTCCAGCA AGTGTAGGCG ACAAATCATC
    TTGTCTCATT TTGAAGACAA ACAACTACGA AAGGCCTCCG TAGGAATTAT GGGAACTGAA
    AACTGCTGTG ATAATTGCAA GTCCAGATTG GATCACTGCT TTTCCCTTGA AGACTCAGAA
    GATACACAGG ACTTTGGTCC GCAAGCATTC CAGTTCTTAT CTGCTGTGAC CATCTTAGGA
    GGAAGGTTCG GCATTGGGAT TCCAATTTTA TTTCTCCGAG GATCTAATTC CCAGCGTCTT
    GAAGATAGAT ACCGCAGCCA CAGTGTCTTT GGTACGGGCA AGGACCGCAC AGAGAAATGG
    TGGAAGGCTC TTTCCCGTCA GCTCATCATT GAGGGATTCT TGGCAGAAGT TCCTGGGCAC
    AGCAAATACG TAAAGATTTG CACCCTTACG GAAAAGGGTA GAAACTGGTT TGCTAAAGCC
    AAAACAGAAT CTCCTCCGAG GCTTTTCCTT CAAACCAATG AACAATTGTG TCCAAGGACA
    TTTCTACCAA GTTCTAAAGC TGTATCTTCA GGCACTGAGG AACATGTCTC TGATCAAGTA
    CCAGTTAAAT TAACTTCAGA GAAGCAGTTT AACTTGGATC AGCTGTATTC TTACAAAGTG
    CATGATAAAA TTTCTTCTGG GAGTAACAGT CCTAAAAACA GCATCATAGT GTCATCACCA
    AGAAAATATT ACAAGTCCTC AGAACCTGTT ATTTCCGCTC AAGAGCAGGA GACTCAGACT
    GCATTATATG GCGAATTGGT AGCAGCTAGG CAGAAACACG CCAATATGAT GGATGTTCCT
    CCGGCTATTC TGGCAACAAA TAAGATATTG TTGGATATGG CCAAAATGAG ACCTACTACA
    GTTGAAAATG TGAAAAGGAT CGATGGTGTT TCTGAAGGCA AAGCTGTGAT GTTGGCCCCT
    CTGTTGGAAG TCATCAGACG TTTCTGTCAA ATAAATAGCG TTCAGACAGA CCTCTTTTCA
    AGCTCAGAAC TACAGGAAGA AGAGAAGAAA AATTTGGTGC TGGAAAATAA AGCAAATTCA
    CTTTCACCGT CTATGGCCAT TACATATTTT TTTTTCCAAG AAAAGAAAAT GTCTTTGAAG
    GATACAGCTG AGAAGAGGAC TCTGCCTCTC ACAGCAGTTG GCATGCACTT ATCCCAGGCG
    ATGAAAGCTG GCTACCCAGT CGATACAGAG CGAGCAGGCC TGACTCCAGA GATTCAAAAG
    ATTATTGCTG ATGTTATCCG AAACCCTCCC ATCAACTCAG ATACAAATAA AATTAACCTA
    ATTAGAATAT ATGTTCCTGA AAACATTGAC ACGTATCTTA TCCACATGAC AATCAAGATC
    CTGGAAAAAG ATGGTGACCA CAGAATGCGA TGCCACTCTT CATGTGATCC CAACAAAAAG
    CGATGTTTCC CCGACCCTGA AGAGAGCTCT TCAAATTCTA AGAGAACCAA GGAAGAAGTA
    GACACTGAGA CCAAGACTTC GTCTGAAATG TCCAAGAGAA AATTACCTTG GTGGTTTGCC
    AAAGGAAATG ACACCTTACC TGATACCAGC AAGAAATCAG TGGCCAAAAC CAAAAACAAG
    GGTCTTTTTA GTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.