POLRMT cDNA ORF clone, Bos taurus(cattle)

The following POLRMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLRMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa235818 XM_010807222.3
Latest version!
Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa235819 XM_005209258.4
Latest version!
Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OBa216137 NM_001205551.2
Latest version!
Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa40058 NM_001205551.3
Latest version!
Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa21399 XM_005209258.3
Latest version!
Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OBa46078 XM_010807222.2
Latest version!
Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
OBa40058 NM_001205551.1 Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa235818
    Clone ID Related Accession (Same CDS sequence) XM_010807222.3
    Accession Version XM_010807222.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC
    TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC
    ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG
    CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG
    GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC
    CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG
    CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG
    AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC
    CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCG CTGGGAAGCG
    CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG
    GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG
    CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC
    CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG
    GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG
    ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG
    GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT
    CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC
    CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC
    ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG
    GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA
    CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG
    CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT
    TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG
    GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA
    GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG
    GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT
    GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC
    CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG
    CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT
    GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG
    GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG
    GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG
    ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT
    CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC
    ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG
    TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG
    ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC
    AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA
    CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT
    GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA
    TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG
    TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG
    CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805524.2
    CDS56..3724
    Translation

    Target ORF information:

    RefSeq Version XM_010807222.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807222.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC
    TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC
    ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG
    CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG
    GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC
    CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG
    CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG
    AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC
    CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCG CTGGGAAGCG
    CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG
    GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG
    CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC
    CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG
    GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG
    ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG
    GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT
    CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC
    CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC
    ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG
    GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA
    CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG
    CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT
    TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG
    GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA
    GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG
    GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT
    GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC
    CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG
    CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT
    GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG
    GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG
    GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG
    ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT
    CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC
    ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG
    TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG
    ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC
    AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA
    CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT
    GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA
    TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG
    TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG
    CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235819
    Clone ID Related Accession (Same CDS sequence) XM_005209258.4
    Accession Version XM_005209258.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209258.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA
    AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC
    ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC
    ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC
    TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC
    GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC
    TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG
    TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209315.2
    CDS31..3696
    Translation

    Target ORF information:

    RefSeq Version XM_005209258.4
    Organism Bos taurus(cattle)
    Definition Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209258.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA
    AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC
    ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC
    ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC
    TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC
    GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC
    TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG
    TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa216137
    Clone ID Related Accession (Same CDS sequence) NM_001205551.2
    Accession Version NM_001205551.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02020222.1. On Jun 30, 2017 this sequence version replaced NM_001205551.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822095 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192480.2
    CDS11..3685
    Translation

    Target ORF information:

    RefSeq Version NM_001205551.2
    Organism Bos taurus(cattle)
    Definition Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205551.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40058
    Clone ID Related Accession (Same CDS sequence) NM_001205551.3 , NM_001205551.1
    Accession Version NM_001205551.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-29
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On May 30, 2018 this sequence version replaced NM_001205551.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145448, SAMN05861950 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192480.3
    CDS11..3685
    Translation

    Target ORF information:

    RefSeq Version NM_001205551.3
    Organism Bos taurus(cattle)
    Definition Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205551.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa40058
    Clone ID Related Accession (Same CDS sequence) NM_001205551.3 , NM_001205551.1
    Accession Version NM_001205551.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-01-03
    Organism Bos taurus(Cattle)
    Product DNA-directed RNA polymerase, mitochondrial
    Comment PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03016644.1 and AAFC03048925.1. On Apr 20, 2011 this sequence version replaced XM_580925.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145448 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001192480.1
    CDS11..3685
    Translation

    Target ORF information:

    RefSeq Version NM_001205551.1
    Organism Bos taurus(Cattle)
    Definition Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001205551.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA
    CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG
    AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC
    TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG
    GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG
    GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC
    AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC
    ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG
    TTCTTTTTCA GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21399
    Clone ID Related Accession (Same CDS sequence) XM_005209258.3
    Accession Version XM_005209258.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA
    AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC
    ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC
    ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC
    TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC
    GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC
    TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG
    TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005209315.1
    CDS48..3713
    Translation

    Target ORF information:

    RefSeq Version XM_005209258.3
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005209258.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG
    TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT
    GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG
    AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG
    ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG
    TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC
    TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG
    AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG
    CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG
    GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC
    AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC
    CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC
    CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG
    CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG
    TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC
    ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG
    GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG
    GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT
    GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC
    TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG
    GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG
    AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA
    CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC
    CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG
    CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC
    ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG
    CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT
    ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC
    TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC
    CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT
    GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC
    AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC
    GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC
    TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC
    CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG
    ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA
    AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC
    ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC
    ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC
    TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC
    GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC
    TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG
    TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC
    AGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa46078
    Clone ID Related Accession (Same CDS sequence) XM_010807222.2
    Accession Version XM_010807222.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3669bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product DNA-directed RNA polymerase, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC
    TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC
    ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG
    CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG
    GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC
    CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG
    CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG
    AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC
    CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCA CTGGGAAGCG
    CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG
    GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG
    CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC
    CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG
    GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG
    ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG
    GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT
    CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC
    CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC
    ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG
    GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA
    CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG
    CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT
    TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG
    GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA
    GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG
    GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT
    GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC
    CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG
    CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT
    GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG
    GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG
    GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG
    ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT
    CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC
    ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG
    TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG
    ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC
    AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA
    CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT
    GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA
    TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG
    TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG
    CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010805524.1
    CDS48..3716
    Translation

    Target ORF information:

    RefSeq Version XM_010807222.2
    Organism Bos taurus(cattle)
    Definition Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010807222.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
    GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG
    AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG
    CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG
    ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG
    CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG
    CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG
    AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC
    AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG
    GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG
    GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC
    CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC
    CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC
    CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC
    CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC
    GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC
    TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG
    GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG
    CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC
    TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC
    ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG
    CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG
    GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC
    CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG
    CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG
    AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC
    CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCA CTGGGAAGCG
    CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG
    GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG
    CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC
    CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG
    GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG
    ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG
    GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT
    CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC
    CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC
    ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG
    GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA
    CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG
    CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT
    TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG
    GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA
    GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG
    GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT
    GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC
    CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG
    CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT
    GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG
    GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG
    GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG
    ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT
    CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC
    ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG
    TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG
    ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC
    AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA
    CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT
    GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA
    TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG
    TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG
    CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT
    TTCAGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.