POLRMT cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLRMT
Entrez Gene ID	504757
Full Name	RNA polymerase mitochondrial
General protein information	Preferred Names RNA polymerase mitochondrial Names DNA-directed RNA polymerase, mitochondrial polymerase (RNA) mitochondrial (DNA directed)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010807222.3	XP_010805524.2	DNA-directed RNA polymerase, mitochondrial isoform X1
XM_005209258.4	XP_005209315.2	DNA-directed RNA polymerase, mitochondrial isoform X2
NM_001205551.3	NP_001192480.3	DNA-directed RNA polymerase, mitochondrial
XM_010807222.2	XP_010805524.1	DNA-directed RNA polymerase, mitochondrial isoform X1
NM_001205551.2	NP_001192480.2	DNA-directed RNA polymerase, mitochondrial
NM_001205551.1	NP_001192480.1	DNA-directed RNA polymerase, mitochondrial
XM_005209258.3	XP_005209315.1	DNA-directed RNA polymerase, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	polrmt	XP_004910992.1
Canis lupus familiaris (dog)	POLRMT	XP_005633227.1
Gallus gallus (chicken)	POLRMT	XP_418204.3
Arabidopsis thaliana (thale cress)	SCA3	NP_179989.1
Oryza sativa (rice)	Os06g0652000	NP_001058231.2
Mus musculus (house mouse)	Polrmt	NP_766139.2
Pan troglodytes (chimpanzee)	POLRMT	XP_003316023.1
Bos taurus (cattle)	POLRMT	NP_001192480.1
Danio rerio (zebrafish)	si:dkey-21e2.1	XP_005170578.1
Homo sapiens (human)	POLRMT	NP_005026.3
Rattus norvegicus (Norway rat)	Polrmt	NP_001100236.1
Drosophila melanogaster (fruit fly)	mtRNApol	NP_001259853.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following POLRMT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLRMT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa235818	XM_010807222.3 Latest version!	Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa235819	XM_005209258.4 Latest version!	Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa216137	NM_001205551.2 Latest version!	Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa40058	NM_001205551.3 Latest version!	Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa21399	XM_005209258.3 Latest version!	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OBa46078	XM_010807222.2 Latest version!	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OBa40058	NM_001205551.1	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLRMT ( XM_010807222.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805524.2 Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa235818
Clone ID Related Accession (Same CDS sequence)	XM_010807222.3
Accession Version	XM_010807222.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010807222.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC 
TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC 
ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG 
CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG 
GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC 
CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG 
CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG 
AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC 
CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCG CTGGGAAGCG 
CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG 
GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG 
CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC 
CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG 
GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG 
ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG 
GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT 
CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC 
CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC 
ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG 
GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA 
CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG 
CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT 
TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG 
GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA 
GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG 
GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT 
GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC 
CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG 
CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT 
GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG 
GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG 
GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG 
ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT 
CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC 
ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG 
TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG 
ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC 
AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA 
CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT 
GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA 
TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG 
TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG 
CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805524.2
CDS	56..3724
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKQDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQRWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	XM_010807222.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807222.3

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC 
TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC 
ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG 
CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG 
GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC 
CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG 
CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG 
AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC 
CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCG CTGGGAAGCG 
CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG 
GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG 
CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC 
CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG 
GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG 
ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG 
GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT 
CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC 
CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC 
ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG 
GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA 
CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG 
CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT 
TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG 
GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA 
GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG 
GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT 
GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC 
CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG 
CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT 
GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG 
GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG 
GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG 
ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT 
CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC 
ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG 
TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG 
ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC 
AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA 
CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT 
GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA 
TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG 
TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG 
CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( XM_005209258.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209315.2 Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa235819
Clone ID Related Accession (Same CDS sequence)	XM_005209258.4
Accession Version	XM_005209258.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005209258.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA 
AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC 
ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC 
ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC 
TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC 
GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC 
TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG 
TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209315.2
CDS	31..3696
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQRWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	XM_005209258.4
Organism	Bos taurus(cattle)
Definition	Bos taurus RNA polymerase mitochondrial (POLRMT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209258.4

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA 
AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC 
ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC 
ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC 
TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC 
GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC 
TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG 
TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( NM_001205551.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192480.2 Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

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CloneID	OBa216137
Clone ID Related Accession (Same CDS sequence)	NM_001205551.2
Accession Version	NM_001205551.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02020222.1. On Jun 30, 2017 this sequence version replaced NM_001205551.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02822095 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192480.2
CDS	11..3685
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQHWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMVRAIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	NM_001205551.2
Organism	Bos taurus(cattle)
Definition	Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205551.2

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( NM_001205551.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192480.3 Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

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CloneID	OBa40058
Clone ID Related Accession (Same CDS sequence)	NM_001205551.3 , NM_001205551.1
Accession Version	NM_001205551.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-29
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000007.1. On May 30, 2018 this sequence version replaced NM_001205551.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145448, SAMN05861950 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192480.3
CDS	11..3685
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQRWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMVRAIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	NM_001205551.3
Organism	Bos taurus(cattle)
Definition	Bos taurus RNA polymerase mitochondrial (POLRMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205551.3

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( NM_001205551.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001192480.1 Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), mRNA.

Price & Availability↑

CloneID	OBa40058
Clone ID Related Accession (Same CDS sequence)	NM_001205551.3 , NM_001205551.1
Accession Version	NM_001205551.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(Cattle)
Product	DNA-directed RNA polymerase, mitochondrial
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03016644.1 and AAFC03048925.1. On Apr 20, 2011 this sequence version replaced XM_580925.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145448 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001192480.1
CDS	11..3685
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQRWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMVRAIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	NM_001205551.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001205551.1

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCGCTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGGTA 
CGTGCCATAA AAGGCGGGCT CCAGAGCCTC ACGTTCAGCA GCAGCGTGGA CACCAACCAG 
AAGCCCAACA CACTGAAGCA GAAGAACGGC TTCCCACCCA ACTTCATCCA CTCACTGGAC 
TCCACACACA TGATGCTCAC CGCCCTGTAC TGTTACAGGA AGGGCCTGAC CTTCGTCTCG 
GTGCATGACT GCTTCTGGAC GCATGCGGCT GATGTCGGGG TCATGAACCA GGTCTGCCGG 
GAGCAATTCG TCCGACTGCA TAGCCAGCCC ATCCTCCATG ACCTGTCCAG GTTCCTCATC 
AAGCGGTTCT GCTCTGATTC TAGAACCTCC AAGAGCATGT GGGTCTCCAG ACTAAAGGAC 
ACTCTGCTGT CTGTGCCTCC GACAGGGACC TTTGACCTGG ACCAGGTGAA GCGTTCCACG 
TTCTTTTTCA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( XM_005209258.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005209315.1 Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X2, mRNA.

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CloneID	OBa21399
Clone ID Related Accession (Same CDS sequence)	XM_005209258.3
Accession Version	XM_005209258.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005209258.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA 
AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC 
ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC 
ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC 
TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC 
GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC 
TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG 
TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005209315.1
CDS	48..3713
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQHWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	XM_005209258.3
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005209258.3

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGGAC CCCTCTGTGT CCTACCCGAA GCTGCACTTG 
TCCCTGAAGG AGCTGCAGGG CCTCTTCCGA CAGCAGCTTG GCGTGGAGAT GGCCACCACT 
GTCACCGTGG AGTCCGTGGA GAAGGCCCCG CTCCTGACCG AGGAGGTCCT GCAGGCGCGG 
AAGACCCTGG CGGCCTTGCG TGCCGAATGG GAGTCGGTGC TGTCCCTGGA GCTGCAGGAG 
ACCAAGGAGA GTGAGGCCCG CGCTGCTGCA GAAGGCCGCC CATCCCTCTT CCCCTACCTG 
TGCCTGCTCA GTGAGAAGGA ATTAGTGGGC TTGCTGCTGC AGACCCTGCA GGCGCTGCCC 
TCACATGGCG AGTCGCTCTT CTTCCTGGCG CACGAGCTGG GCGTGCGTGT GTTGAAGAGG 
AAGCGGCTCA GGAACCAGGT AGAAGAGCTG GAGCAGCGCT ACTTCGAGTA CCTGCACCTG 
CTGGCCTCGG ACACCCAGGT AGCAGAGCCC TGCCTGCCTC GCCAGCACTG GGAAGCGCTG 
GGCGTGCCTG AGGCCCCCCC GGAGCAGCCC TGGCCTGTGT CTGTGCTAGT GCAGCTGGGC 
AAGCAGCTGG CAGAGGTGCT GGTGCGAGCC GTGCAGATGC CCAGCAGCCT GGCAGCGCCC 
CGGGGCCCCA GCACACTCAT CCCTGTGCTC TACCACGTGT ATTCTTTCCG CAGTTTCCGC 
CAGATCGGGA TCCTGAAGCC GCACCCAGCC TTCACGCAGC TGCAGGAGAC AGCGGCGGAG 
CGCACGCTGA TCTTCGAGTC CACGGAGGTG CCCATGCTGT GCCCGCCCCT CCCCTGGACG 
TCACCACACT CGGGCGCCTT CCTGCTGAGC CCCACCAAGA TGATGCGGTC CTTGGAGGGC 
ACCCAGCAGC ACCAGCTCCT GTTGGAGCGC TGCCCACCCG CTGAGCTGCA TGGCGCTCTG 
GATGCCCTCA CTCAGCTGGG CAACTGTGCT TGGCGCGTCA ATGGGCGCGT GCTTGACCTG 
GTGCTTGACC TCTTCAGGGA CAAGGGCTGC CCCCGCCTGG GCGTGCCCGC CCCACCCACT 
GAGGCCCCCC GGCCCCCCGA GGGCCGCCAG ACCCCCAAGG GCTGCCTGCT GCCCGAGGCC 
TCACCCGCTG ACAAGGCGGA GATGCGGCGG GAGCTGGCAC GGCGGCTGAA GGTGGCACGG 
GAGATGCAGA GCCTGCGCGC AGATGCACTC TACCGCCTGT CGCTGGCCCA GCATCTGCGG 
AACCACGTCT TCTGGCTGCC GCATAACATG GACTTCCGAG GCCGTACCTA CCCCTGTTCA 
CCACACTTCA ACCACCTCGG CAGTGACCTG GCCCGCGCGC TGCTGGAGTT TGCCCAGGGC 
CGCCCACTTG GCCCCCATGG CCTCGACTGG CTCAAGATCC ACCTGATCAA CCTCACAGGG 
CTCAAGAAGC GCGAGTCACT ACAGACGCGC CGCGACTACG CAGACGCGGT GATGGAGGAC 
ATCCTGGACT CAGCTGAACG ACCTATGACG GGCCGGAAGT GGTGGATGGA GGCAGATGAG 
CCCTGGCAAA CCCTGGCCTG CTGCATGGAG ATTGCCCAGG TGGTGCATTC CCCCGACCCT 
ACCACCTACA TCTCCCACTT TCCGGTTCAC CAGGACGGCT CCTGTAACGG CCTCCAGCAC 
TATGCCGCCC TGGGCCGGGA CAGCACGGGG GCCACCTCTG TCAACCTGTC GCCCTCTGAC 
CTGCCGCAGG ACGTGTACAG TGAGGTAGCT GCACAGGTGG AGGTATTCCG CAGGCAGGAT 
GCCAAGCAGG GAGTGCGGGT GGCCCAGGTG CTGGAGGGCT TCATTAGCCG CAAGGTGGTC 
AAGCAGACAG TGATGACCGT GGTGTACGGC GTCACCCGCT ACGGCGGCCG CCTGCAGATC 
GAGAAGCGCC TCCGGGAGCT CGAGGACTTC CCCCAGGAGT TCGTGTGGGA GGCATCTCAC 
TACCTTGTGC GCCAGGTGTT CAACAGCCTC CAGGAGATGT TCTCTGGCAC CCGGTCCATC 
CAGCGCTGGC TGACTGAGAG TGCCCGGCTC ATCTCACTCA CGGGCTCGGC TGTGGAGTGG 
ATCACGCCCC TGGGTGTCCC CATCATCCAG CCCTACCACC GAGACTCCAA GGTCATGATA 
AAAGGCGGGC TCCAGAGCCT CACGTTCAGC AGCAGCGTGG ACACCAACCA GAAGCCCAAC 
ACACTGAAGC AGAAGAACGG CTTCCCACCC AACTTCATCC ACTCACTGGA CTCCACACAC 
ATGATGCTCA CCGCCCTGTA CTGTTACAGG AAGGGCCTGA CCTTCGTCTC GGTGCATGAC 
TGCTTCTGGA CGCATGCGGC TGATGTCGGG GTCATGAACC AGGTCTGCCG GGAGCAATTC 
GTCCGACTGC ATAGCCAGCC CATCCTCCAT GACCTGTCCA GGTTCCTCAT CAAGCGGTTC 
TGCTCTGATT CTAGAACCTC CAAGAGCATG TGGGTCTCCA GACTAAAGGA CACTCTGCTG 
TCTGTGCCTC CGACAGGGAC CTTTGACCTG GACCAGGTGA AGCGTTCCAC GTTCTTTTTC 
AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLRMT ( XM_010807222.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010805524.1 Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X1, mRNA.

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CloneID	OBa46078
Clone ID Related Accession (Same CDS sequence)	XM_010807222.2
Accession Version	XM_010807222.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	DNA-directed RNA polymerase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104031.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010807222.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC 
TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC 
ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG 
CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG 
GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC 
CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG 
CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG 
AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC 
CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCA CTGGGAAGCG 
CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG 
GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG 
CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC 
CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG 
GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG 
ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG 
GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT 
CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC 
CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC 
ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG 
GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA 
CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG 
CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT 
TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG 
GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA 
GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG 
GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT 
GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC 
CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG 
CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT 
GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG 
GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG 
GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG 
ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT 
CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC 
ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG 
TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG 
ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC 
AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA 
CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT 
GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA 
TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG 
TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG 
CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010805524.1
CDS	48..3716
Translation	MSALRCGRRAAGLRRALWPKGHPCFLAEEGTLGGVWGRKRSLSASACEQDRRKGCGHAELLEVLEARVRQLQAEGTAEEVMVKRVAMAQAPEAGGIWPPRQAPVGAEGAAPVLSSHWVQKLDKDQQLIEKRLQQLEGKLPKIVEKSTWEKRLRMEPRLLSRKLAGQLQQWERKRPQSPWEEQLARLLQEAPKKLSREADEGSESRLEGQRQKLLAFLECCLLTGHLPLAHHVLVAQHSRAPQQQLLTLPMYNTVMLGWARQGSFKELMYVFFMLKDAGLAPDLLSYAAALQCMGRLDQDAGTIRRCLKQMEQDGLSLQGLFSAVPLSTEEREELLRAVRKARPAFSPQPPPPPPPQVNTSPLLQEIYAKQDPSVSYPKLHLSLKELQGLFRQQLGVEMATTVTVESVEKAPLLTEEVLQARKTLAALRAEWESVLSLELQETKESEARAAAEGRPSLFPYLCLLSEKELVGLLLQTLQALPSHGESLFFLAHELGVRVLKRKRLRNQVEELEQRYFEYLHLLASDTQVAEPCLPRQHWEALGVPEAPPEQPWPVSVLVQLGKQLAEVLVRAVQMPSSLAAPRGPSTLIPVLYHVYSFRSFRQIGILKPHPAFTQLQETAAERTLIFESTEVPMLCPPLPWTSPHSGAFLLSPTKMMRSLEGTQQHQLLLERCPPAELHGALDALTQLGNCAWRVNGRVLDLVLDLFRDKGCPRLGVPAPPTEAPRPPEGRQTPKGCLLPEASPADKAEMRRELARRLKVAREMQSLRADALYRLSLAQHLRNHVFWLPHNMDFRGRTYPCSPHFNHLGSDLARALLEFAQGRPLGPHGLDWLKIHLINLTGLKKRESLQTRRDYADAVMEDILDSAERPMTGRKWWMEADEPWQTLACCMEIAQVVHSPDPTTYISHFPVHQDGSCNGLQHYAALGRDSTGATSVNLSPSDLPQDVYSEVAAQVEVFRRQDAKQGVRVAQVLEGFISRKVVKQTVMTVVYGVTRYGGRLQIEKRLRELEDFPQEFVWEASHYLVRQVFNSLQEMFSGTRSIQRWLTESARLISLTGSAVEWITPLGVPIIQPYHRDSKVMIKGGLQSLTFSSSVDTNQKPNTLKQKNGFPPNFIHSLDSTHMMLTALYCYRKGLTFVSVHDCFWTHAADVGVMNQVCREQFVRLHSQPILHDLSRFLIKRFCSDSRTSKSMWVSRLKDTLLSVPPTGTFDLDQVKRSTFFFS

Target ORF information:

RefSeq Version	XM_010807222.2
Organism	Bos taurus(cattle)
Definition	Bos taurus polymerase (RNA) mitochondrial (DNA directed) (POLRMT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010807222.2

ORF Insert Sequence:

ATGTCGGCGC TGCGCTGTGG CCGCCGGGCT GCGGGGCTTC GGAGGGCCCT GTGGCCGAAG 
GGCCACCCCT GCTTCCTTGC CGAAGAAGGG ACCCTTGGTG GCGTCTGGGG CCGGAAGAGG 
AGCTTGTCTG CCAGCGCTTG TGAGCAGGAC CGCCGGAAGG GCTGCGGCCA CGCGGAACTG 
CTGGAGGTGC TCGAGGCACG GGTGCGGCAA CTGCAGGCTG AGGGCACGGC AGAGGAGGTG 
ATGGTGAAGA GGGTGGCCAT GGCTCAGGCC CCGGAGGCTG GTGGCATCTG GCCCCCCAGG 
CAGGCCCCAG TGGGGGCTGA AGGGGCTGCC CCCGTGCTCA GCAGCCACTG GGTACAGAAG 
CTGGACAAGG ATCAGCAGCT GATAGAGAAG CGCTTGCAGC AGCTGGAGGG GAAGCTGCCG 
AAGATCGTGG AGAAGTCGAC CTGGGAGAAG CGTCTGCGCA TGGAGCCTCG GCTGCTGAGC 
AGGAAGCTGG CGGGCCAGCT GCAGCAGTGG GAGCGCAAGA GGCCGCAGAG CCCCTGGGAG 
GAGCAGCTGG CCCGGCTGCT GCAGGAGGCC CCGAAGAAGC TGAGCCGGGA GGCAGATGAG 
GGCTCCGAAT CCCGGCTGGA GGGCCAGCGC CAGAAGCTCC TGGCCTTCCT GGAGTGCTGC 
CTGCTCACAG GCCACCTGCC CCTGGCCCAC CACGTGCTGG TTGCACAGCA CAGCCGGGCC 
CCACAGCAGC AGCTGCTCAC ACTCCCCATG TACAACACCG TCATGCTTGG CTGGGCTCGC 
CAGGGCTCCT TCAAAGAGCT GATGTATGTG TTCTTCATGC TGAAAGATGC CGGCCTGGCC 
CCGGACCTGC TGTCCTACGC AGCCGCCCTG CAGTGCATGG GGCGCCTGGA CCAGGATGCC 
GGCACCATCC GAAGGTGCCT GAAGCAGATG GAGCAGGACG GGCTGTCGCT GCAGGGGCTC 
TTCTCAGCCG TGCCCCTGAG CACGGAGGAG CGGGAAGAGC TCCTGCGTGC TGTGCGCAAG 
GCCAGACCTG CCTTCAGCCC CCAGCCGCCA CCCCCGCCCC CACCCCAGGT CAACACCTCG 
CCACTGCTCC AGGAGATCTA CGCCAAGCAG GACCCCTCTG TGTCCTACCC GAAGCTGCAC 
TTGTCCCTGA AGGAGCTGCA GGGCCTCTTC CGACAGCAGC TTGGCGTGGA GATGGCCACC 
ACTGTCACCG TGGAGTCCGT GGAGAAGGCC CCGCTCCTGA CCGAGGAGGT CCTGCAGGCG 
CGGAAGACCC TGGCGGCCTT GCGTGCCGAA TGGGAGTCGG TGCTGTCCCT GGAGCTGCAG 
GAGACCAAGG AGAGTGAGGC CCGCGCTGCT GCAGAAGGCC GCCCATCCCT CTTCCCCTAC 
CTGTGCCTGC TCAGTGAGAA GGAATTAGTG GGCTTGCTGC TGCAGACCCT GCAGGCGCTG 
CCCTCACATG GCGAGTCGCT CTTCTTCCTG GCGCACGAGC TGGGCGTGCG TGTGTTGAAG 
AGGAAGCGGC TCAGGAACCA GGTAGAAGAG CTGGAGCAGC GCTACTTCGA GTACCTGCAC 
CTGCTGGCCT CGGACACCCA GGTAGCAGAG CCCTGCCTGC CTCGCCAGCA CTGGGAAGCG 
CTGGGCGTGC CTGAGGCCCC CCCGGAGCAG CCCTGGCCTG TGTCTGTGCT AGTGCAGCTG 
GGCAAGCAGC TGGCAGAGGT GCTGGTGCGA GCCGTGCAGA TGCCCAGCAG CCTGGCAGCG 
CCCCGGGGCC CCAGCACACT CATCCCTGTG CTCTACCACG TGTATTCTTT CCGCAGTTTC 
CGCCAGATCG GGATCCTGAA GCCGCACCCA GCCTTCACGC AGCTGCAGGA GACAGCGGCG 
GAGCGCACGC TGATCTTCGA GTCCACGGAG GTGCCCATGC TGTGCCCGCC CCTCCCCTGG 
ACGTCACCAC ACTCGGGCGC CTTCCTGCTG AGCCCCACCA AGATGATGCG GTCCTTGGAG 
GGCACCCAGC AGCACCAGCT CCTGTTGGAG CGCTGCCCAC CCGCTGAGCT GCATGGCGCT 
CTGGATGCCC TCACTCAGCT GGGCAACTGT GCTTGGCGCG TCAATGGGCG CGTGCTTGAC 
CTGGTGCTTG ACCTCTTCAG GGACAAGGGC TGCCCCCGCC TGGGCGTGCC CGCCCCACCC 
ACTGAGGCCC CCCGGCCCCC CGAGGGCCGC CAGACCCCCA AGGGCTGCCT GCTGCCCGAG 
GCCTCACCCG CTGACAAGGC GGAGATGCGG CGGGAGCTGG CACGGCGGCT GAAGGTGGCA 
CGGGAGATGC AGAGCCTGCG CGCAGATGCA CTCTACCGCC TGTCGCTGGC CCAGCATCTG 
CGGAACCACG TCTTCTGGCT GCCGCATAAC ATGGACTTCC GAGGCCGTAC CTACCCCTGT 
TCACCACACT TCAACCACCT CGGCAGTGAC CTGGCCCGCG CGCTGCTGGA GTTTGCCCAG 
GGCCGCCCAC TTGGCCCCCA TGGCCTCGAC TGGCTCAAGA TCCACCTGAT CAACCTCACA 
GGGCTCAAGA AGCGCGAGTC ACTACAGACG CGCCGCGACT ACGCAGACGC GGTGATGGAG 
GACATCCTGG ACTCAGCTGA ACGACCTATG ACGGGCCGGA AGTGGTGGAT GGAGGCAGAT 
GAGCCCTGGC AAACCCTGGC CTGCTGCATG GAGATTGCCC AGGTGGTGCA TTCCCCCGAC 
CCTACCACCT ACATCTCCCA CTTTCCGGTT CACCAGGACG GCTCCTGTAA CGGCCTCCAG 
CACTATGCCG CCCTGGGCCG GGACAGCACG GGGGCCACCT CTGTCAACCT GTCGCCCTCT 
GACCTGCCGC AGGACGTGTA CAGTGAGGTA GCTGCACAGG TGGAGGTATT CCGCAGGCAG 
GATGCCAAGC AGGGAGTGCG GGTGGCCCAG GTGCTGGAGG GCTTCATTAG CCGCAAGGTG 
GTCAAGCAGA CAGTGATGAC CGTGGTGTAC GGCGTCACCC GCTACGGCGG CCGCCTGCAG 
ATCGAGAAGC GCCTCCGGGA GCTCGAGGAC TTCCCCCAGG AGTTCGTGTG GGAGGCATCT 
CACTACCTTG TGCGCCAGGT GTTCAACAGC CTCCAGGAGA TGTTCTCTGG CACCCGGTCC 
ATCCAGCGCT GGCTGACTGA GAGTGCCCGG CTCATCTCAC TCACGGGCTC GGCTGTGGAG 
TGGATCACGC CCCTGGGTGT CCCCATCATC CAGCCCTACC ACCGAGACTC CAAGGTCATG 
ATAAAAGGCG GGCTCCAGAG CCTCACGTTC AGCAGCAGCG TGGACACCAA CCAGAAGCCC 
AACACACTGA AGCAGAAGAA CGGCTTCCCA CCCAACTTCA TCCACTCACT GGACTCCACA 
CACATGATGC TCACCGCCCT GTACTGTTAC AGGAAGGGCC TGACCTTCGT CTCGGTGCAT 
GACTGCTTCT GGACGCATGC GGCTGATGTC GGGGTCATGA ACCAGGTCTG CCGGGAGCAA 
TTCGTCCGAC TGCATAGCCA GCCCATCCTC CATGACCTGT CCAGGTTCCT CATCAAGCGG 
TTCTGCTCTG ATTCTAGAAC CTCCAAGAGC ATGTGGGTCT CCAGACTAAA GGACACTCTG 
CTGTCTGTGC CTCCGACAGG GACCTTTGAC CTGGACCAGG TGAAGCGTTC CACGTTCTTT 
TTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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POLRMT cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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