DDX4 cDNA ORF clone, Bos taurus(cattle)

The following DDX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa00146 NM_001278644.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa01123 NM_001278643.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa41576 NM_001007819.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa00146 XM_024981231.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa01123 XM_024981230.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OBa45840 XM_024981227.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OBa45841 XM_024981229.1
Latest version!
Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa00146
    Clone ID Related Accession (Same CDS sequence) XM_024981231.1 , NM_001278644.1
    Accession Version NM_001278644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform 3
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126699.1 and JX437189.1. Transcript Variant: This variant (3) lacks an exon and represents use of an alternate splice donor site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT
    TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC
    AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT
    GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT
    TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA
    TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA
    GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG
    TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA
    CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT
    GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG
    ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT
    ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG
    ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT
    CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT
    ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT
    TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA
    ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT
    GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT
    GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA
    ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT
    CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG
    GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA
    GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA
    ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA
    AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT
    GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT
    GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT
    GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA
    GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT
    GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA
    GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC
    ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265573.1
    CDS27..2066
    Translation

    Target ORF information:

    RefSeq Version NM_001278644.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT
    TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC
    AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT
    GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT
    TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA
    TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA
    GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG
    TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA
    CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT
    GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG
    ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT
    ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG
    ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT
    CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT
    ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT
    TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA
    ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT
    GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT
    GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA
    ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT
    CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG
    GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA
    GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA
    ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA
    AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT
    GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT
    GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT
    GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA
    GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT
    GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA
    GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC
    ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01123
    Clone ID Related Accession (Same CDS sequence) NM_001278643.1 , XM_024981230.1
    Accession Version NM_001278643.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-21
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126699.1 and JX437188.1. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT
    TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT
    GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC
    AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG
    TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT
    CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT
    AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT
    GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG
    AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG
    ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA
    GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA
    CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT
    AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA
    GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG
    CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG
    GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA
    GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG
    TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG
    AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT
    TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT
    TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT
    CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT
    GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG
    TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG
    GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA
    ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA
    GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG
    GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT
    GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT
    GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA
    CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT
    GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG
    AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG
    TCATGGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001265572.1
    CDS27..2138
    Translation

    Target ORF information:

    RefSeq Version NM_001278643.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001278643.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT
    TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT
    GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC
    AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG
    TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT
    CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT
    AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT
    GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG
    AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG
    ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA
    GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA
    CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT
    AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA
    GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG
    CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG
    GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA
    GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG
    TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG
    AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT
    TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT
    TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT
    CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT
    GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG
    TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG
    GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA
    ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA
    GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG
    GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT
    GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT
    GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA
    CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT
    GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG
    AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG
    TCATGGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa41576
    Clone ID Related Accession (Same CDS sequence) NM_001007819.1
    Accession Version NM_001007819.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF541971.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF541971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT
    ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT
    AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA
    GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT
    TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA
    GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT
    GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC
    CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA
    ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC
    ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC
    TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA
    TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA
    CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT
    ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG
    ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT
    CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC
    AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC
    ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA
    TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA
    CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA
    GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT
    ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG
    AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGACGT TCAGCAGACC
    ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA
    GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT
    TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA
    AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA
    GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT
    TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC
    AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA
    GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA
    TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA
    AAGAATTACC CCGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT
    AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001007820.1
    CDS24..2213
    Translation

    Target ORF information:

    RefSeq Version NM_001007819.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001007819.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT
    ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT
    AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA
    GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT
    TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA
    GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT
    GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC
    CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA
    ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC
    ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC
    TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA
    TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA
    CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT
    ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG
    ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT
    CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC
    AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC
    ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA
    TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA
    CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA
    GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT
    ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG
    AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGACGT TCAGCAGACC
    ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA
    GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT
    TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA
    AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA
    GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT
    TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC
    AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA
    GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA
    TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA
    AAGAATTACC CCGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT
    AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa00146
    Clone ID Related Accession (Same CDS sequence) XM_024981231.1 , NM_001278644.1
    Accession Version XM_024981231.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2040bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT
    TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC
    AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT
    GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT
    TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA
    TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA
    GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG
    TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA
    CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT
    GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG
    ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT
    ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG
    ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT
    CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT
    ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT
    TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA
    ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT
    GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT
    GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA
    ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT
    CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG
    GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA
    GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA
    ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA
    AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT
    GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT
    GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT
    GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA
    GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT
    GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA
    GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC
    ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836999.1
    CDS222..2261
    Translation

    Target ORF information:

    RefSeq Version XM_024981231.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981231.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT
    TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC
    AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT
    GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT
    TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA
    TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA
    GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG
    TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA
    CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT
    GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG
    ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT
    ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG
    ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT
    CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT
    ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT
    TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA
    ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT
    GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT
    GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA
    ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT
    CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG
    GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA
    GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA
    ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA
    AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT
    GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT
    GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT
    GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA
    GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT
    GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA
    GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC
    ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa01123
    Clone ID Related Accession (Same CDS sequence) NM_001278643.1 , XM_024981230.1
    Accession Version XM_024981230.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2112bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT
    TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT
    GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC
    AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG
    TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT
    CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT
    AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT
    GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG
    AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG
    ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA
    GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA
    CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT
    AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA
    GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG
    CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG
    GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA
    GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG
    TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG
    AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT
    TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT
    TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT
    CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT
    GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG
    TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG
    GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA
    ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA
    GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG
    GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT
    GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT
    GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA
    CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT
    GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG
    AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG
    TCATGGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836998.1
    CDS223..2334
    Translation

    Target ORF information:

    RefSeq Version XM_024981230.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981230.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT
    TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT
    GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC
    AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG
    TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT
    CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT
    AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT
    GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG
    AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG
    ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA
    GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA
    CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT
    AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA
    GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG
    CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG
    GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA
    GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG
    TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG
    AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT
    TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT
    TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT
    CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT
    GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG
    TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG
    GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA
    ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA
    GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG
    GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT
    GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT
    GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA
    CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT
    GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG
    AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG
    TCATGGGATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45840
    Clone ID Related Accession (Same CDS sequence) XM_024981227.1
    Accession Version XM_024981227.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2190bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT
    ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT
    AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA
    GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT
    TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA
    GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT
    GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC
    CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA
    ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC
    ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC
    TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA
    TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA
    CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT
    ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG
    ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT
    CGTTTTAAAG AACTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC
    AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC
    ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA
    TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA
    CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA
    GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT
    ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG
    AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGATGT TCAGCAGACC
    ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA
    GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT
    TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA
    AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA
    GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT
    TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC
    AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA
    GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA
    TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA
    AAGAATTACC ACGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT
    AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836995.1
    CDS224..2413
    Translation

    Target ORF information:

    RefSeq Version XM_024981227.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981227.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT
    ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT
    AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA
    GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT
    TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA
    GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT
    GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC
    CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA
    ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC
    ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC
    TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA
    TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA
    CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT
    ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG
    ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT
    CGTTTTAAAG AACTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC
    AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC
    ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA
    TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA
    CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA
    GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT
    ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG
    AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGATGT TCAGCAGACC
    ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA
    GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT
    TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA
    AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA
    GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT
    TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC
    AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA
    GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA
    TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA
    AAGAATTACC ACGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT
    AATCCAGTAG ACGATGAGTC ATGGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45841
    Clone ID Related Accession (Same CDS sequence) XM_024981229.1
    Accession Version XM_024981229.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2118bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product probable ATP-dependent RNA helicase DDX4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTT TTTCAAATAA CAAGTTTGAA
    GAAGGTGATA GCTCTGGTTT CTGGAGAGAA TCTAGTATTG ACTGTGAAGA TAATCAAACA
    CGGAACAGAG GGTTTTCCAA GAGAGGCGGT TATCAAGACG GAAATGATTC AGAAGCTTTA
    GGATCGTCCA GAAGAGGTGG AAGAGGTAGT TTCCGAGGTT GCCGTGGAGG ATTTGGTCGA
    GGAAGTCCAA ATAGTGATTA TGAACAAGAT GAGGGGACCC AGCGCTCTGG TGGCATTTTT
    GGTTCTAGAA GATCAGCATT AAGTGGTGCA GGTAATGGTG ACACTTTCCA AAGCAGAAGT
    GGTGGTGGAA GTGGACGAGG TGGTTACAAA GGTTTAAATG AAGAAGTAAT TACAGGCTCT
    GGAAAGAATT CTTGGAAGTC AGAAGCTGAA GGAGGAGAAA GTGGTGACAC TCAAGGACCA
    AAAGTGACCT ACATACCACC TCCTCCACCT GAAGATGAGG ACTCCATCTT TGCACATTAT
    CAAACAGGCA TAAACTTTGA CAAATACGAT ACTATTCTTG TAGAAGTATC TGGACATGAT
    CCACCACCAG CAATTCTGAC TTTTGAAGAA GCTAATCTTT GTCAGACACT GAATAACAAC
    ATTGCTAAAG CTGGTTATAC TAAGCTTACT CCTGTGCAAA AATACAGTAT TCCTATTATA
    CAAGGAGGAC GAGATTTGAT GGCTTGTGCT CAGACGGGCT CTGGAAAGAC TGCAGCTTTT
    CTCTTGCCAA TTTTGGCTCA TATGATGCGT GATGGAATAA CTGCCAGTCG TTTTAAAGAA
    CTGCAGGAAC CAGAGTGTAT TATTGTAGCA CCAACTCGAG AGTTGATCAA TCAGATCTAT
    TTGGAAGCCA GAAAATTTTC TTTTGGGACT TGTGTAAGAG CAGTTGTCAT ATATGGGGGA
    ACCCAGTTGG GGCATTCAAT TCGACAAATA GTACAAGGCT GTAATATATT ATGTGCTACT
    CCTGGGAGAC TGATGGATGT CATAGGCAAA GAAAAGATTG GTCTCAGACA AGTCAAATAC
    TTAGTTTTGG ATGAAGCTGA TCGCATGCTG GATATGGGTT TTGGACCAGA GATGAAAAAG
    TTAATTTCCT GCCCAGGAAT GCCATCAAAG GAACAGCGTC AGACCCTTAT GTTCAGTGCA
    ACTTTTCCAG AAGAAATTCA AAGGTTGGCT GGGGAGTTTT TAAAGTCGAA TTATTTGTTT
    GTTGCTGTTG GACAAGTGGG TGGAGCATGT AGAGATGTTC AGCAGACCAT TCTTCAAGTT
    GGCCAGTACT CAAAAAGAGA AAAACTTGTT GAAATTCTAC GAAACATAGG TGATGAAAGA
    ACTATGGTCT TTGTTGAAAC TAAGAAAAAA GCAGATTTTA TTGCCACTTT TCTTTGTCAA
    GAAAAAATAT CAACAACAAG TATTCATGGT GATCGTGAAC AGAGAGAAAG AGAGCAAGCT
    CTTGGAGATT TCCGCTGTGG AAAGTGCCCT GTTCTTGTTG CTACTTCAGT AGCTGCCCGA
    GGGCTGGATA TTGAAAATGT TCAGCATGTT ATTAATTTTG ATCTTCCTTC TACCATTGAT
    GAATATGTTC ATCGAATTGG GCGTACTGGT CGTTGTGGAA ATACTGGCAG AGCTATTTCC
    TTTTTTGATC TGGAATCAGA TAGCCAGTTA GCACAGCCTC TAGTGAAAGT GCTATCAGAT
    GCTCAACAGG ATGTTCCTGC ATGGTTAGAA GAAATTGCCT TTAGTACATA TGGTCCTGGC
    TTCAGTGGTA ATGCAAGAGG AAATGTGTTT GCATCAGTCG ATACTAGAAA GAATTACCAC
    GGCAAGAGCT CTTTGAACAC AGCAGGGTTT TCTTCAACAC AAGCTCCTAA TCCAGTAGAC
    GATGAGTCAT GGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836997.1
    CDS150..2267
    Translation

    Target ORF information:

    RefSeq Version XM_024981229.1
    Organism Bos taurus(cattle)
    Definition Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981229.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
    GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG
    GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA
    TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTT TTTCAAATAA CAAGTTTGAA
    GAAGGTGATA GCTCTGGTTT CTGGAGAGAA TCTAGTATTG ACTGTGAAGA TAATCAAACA
    CGGAACAGAG GGTTTTCCAA GAGAGGCGGT TATCAAGACG GAAATGATTC AGAAGCTTTA
    GGATCGTCCA GAAGAGGTGG AAGAGGTAGT TTCCGAGGTT GCCGTGGAGG ATTTGGTCGA
    GGAAGTCCAA ATAGTGATTA TGAACAAGAT GAGGGGACCC AGCGCTCTGG TGGCATTTTT
    GGTTCTAGAA GATCAGCATT AAGTGGTGCA GGTAATGGTG ACACTTTCCA AAGCAGAAGT
    GGTGGTGGAA GTGGACGAGG TGGTTACAAA GGTTTAAATG AAGAAGTAAT TACAGGCTCT
    GGAAAGAATT CTTGGAAGTC AGAAGCTGAA GGAGGAGAAA GTGGTGACAC TCAAGGACCA
    AAAGTGACCT ACATACCACC TCCTCCACCT GAAGATGAGG ACTCCATCTT TGCACATTAT
    CAAACAGGCA TAAACTTTGA CAAATACGAT ACTATTCTTG TAGAAGTATC TGGACATGAT
    CCACCACCAG CAATTCTGAC TTTTGAAGAA GCTAATCTTT GTCAGACACT GAATAACAAC
    ATTGCTAAAG CTGGTTATAC TAAGCTTACT CCTGTGCAAA AATACAGTAT TCCTATTATA
    CAAGGAGGAC GAGATTTGAT GGCTTGTGCT CAGACGGGCT CTGGAAAGAC TGCAGCTTTT
    CTCTTGCCAA TTTTGGCTCA TATGATGCGT GATGGAATAA CTGCCAGTCG TTTTAAAGAA
    CTGCAGGAAC CAGAGTGTAT TATTGTAGCA CCAACTCGAG AGTTGATCAA TCAGATCTAT
    TTGGAAGCCA GAAAATTTTC TTTTGGGACT TGTGTAAGAG CAGTTGTCAT ATATGGGGGA
    ACCCAGTTGG GGCATTCAAT TCGACAAATA GTACAAGGCT GTAATATATT ATGTGCTACT
    CCTGGGAGAC TGATGGATGT CATAGGCAAA GAAAAGATTG GTCTCAGACA AGTCAAATAC
    TTAGTTTTGG ATGAAGCTGA TCGCATGCTG GATATGGGTT TTGGACCAGA GATGAAAAAG
    TTAATTTCCT GCCCAGGAAT GCCATCAAAG GAACAGCGTC AGACCCTTAT GTTCAGTGCA
    ACTTTTCCAG AAGAAATTCA AAGGTTGGCT GGGGAGTTTT TAAAGTCGAA TTATTTGTTT
    GTTGCTGTTG GACAAGTGGG TGGAGCATGT AGAGATGTTC AGCAGACCAT TCTTCAAGTT
    GGCCAGTACT CAAAAAGAGA AAAACTTGTT GAAATTCTAC GAAACATAGG TGATGAAAGA
    ACTATGGTCT TTGTTGAAAC TAAGAAAAAA GCAGATTTTA TTGCCACTTT TCTTTGTCAA
    GAAAAAATAT CAACAACAAG TATTCATGGT GATCGTGAAC AGAGAGAAAG AGAGCAAGCT
    CTTGGAGATT TCCGCTGTGG AAAGTGCCCT GTTCTTGTTG CTACTTCAGT AGCTGCCCGA
    GGGCTGGATA TTGAAAATGT TCAGCATGTT ATTAATTTTG ATCTTCCTTC TACCATTGAT
    GAATATGTTC ATCGAATTGG GCGTACTGGT CGTTGTGGAA ATACTGGCAG AGCTATTTCC
    TTTTTTGATC TGGAATCAGA TAGCCAGTTA GCACAGCCTC TAGTGAAAGT GCTATCAGAT
    GCTCAACAGG ATGTTCCTGC ATGGTTAGAA GAAATTGCCT TTAGTACATA TGGTCCTGGC
    TTCAGTGGTA ATGCAAGAGG AAATGTGTTT GCATCAGTCG ATACTAGAAA GAATTACCAC
    GGCAAGAGCT CTTTGAACAC AGCAGGGTTT TCTTCAACAC AAGCTCCTAA TCCAGTAGAC
    GATGAGTCAT GGGATTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.