DDX4 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	DDX4
Entrez Gene ID	493725
Full Name	DEAD-box helicase 4
Synonyms	VASA
General protein information	Preferred Names DEAD-box helicase 4 Names probable ATP-dependent RNA helicase DDX4 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 DEAD box protein 4
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024981229.1	XP_024836997.1	probable ATP-dependent RNA helicase DDX4 isoform X2
XM_024981227.1	XP_024836995.1	probable ATP-dependent RNA helicase DDX4 isoform X1
XM_024981231.1	XP_024836999.1	probable ATP-dependent RNA helicase DDX4 isoform X4
NM_001278643.1	NP_001265572.1	probable ATP-dependent RNA helicase DDX4 isoform 2
NM_001007819.1	NP_001007820.1	probable ATP-dependent RNA helicase DDX4 isoform 1
XM_024981230.1	XP_024836998.1	probable ATP-dependent RNA helicase DDX4 isoform X3
NM_001278644.1	NP_001265573.1	probable ATP-dependent RNA helicase DDX4 isoform 3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DDX4	XP_517757.3
Canis lupus familiaris (dog)	DDX4	XP_005617450.1
Gallus gallus (chicken)	DDX4	NP_990039.1
Homo sapiens (human)	DDX4	NP_077726.1
Bos taurus (cattle)	DDX4	NP_001007820.1
	DDX4	XP_001099856.2
Mus musculus (house mouse)	Ddx4	NP_001139357.1
Rattus norvegicus (Norway rat)	Ddx4	NP_001071115.1
Danio rerio (zebrafish)	vasa	NP_571132.1
Drosophila melanogaster (fruit fly)	vas	NP_001260458.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008578	XP_314684.3
Xenopus tropicalis (tropical clawed frog)	ddx4	NP_001016823.1

Gene Ontology
Bibliography

Related articles in PubMed

Bone morphogenetic protein 4 and retinoic acid trigger bovine VASA homolog expression in differentiating bovine induced pluripotent stem cells.
Malaver-Ortega LF, Sumer H, Jain K, Verma PJ
Molecular reproduction and development83(2)149-61(2016 Feb)

Comparison of the efficacy of three concentrations of retinoic acid for transdifferentiation induction in sheep marrow-derived mesenchymal stem cells into male germ cells.
Ghasemzadeh-Hasankolaei M, Eslaminejad MB, Batavani R, Sedighi-Gilani M
Andrologia46(1)24-35(2014 Feb)

Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis.
Luo H, Zhou Y, Li Y, Li Q
BMC genetics1458(2013 Jul)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Identification, localization, and sequencing of fetal bovine VASA homolog.
Bartholomew RA, Parks JE
Animal reproduction science101(3-4)241-51(2007 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Bone morphogenetic protein 4 and retinoic acid trigger bovine VASA homolog expression in differentiating bovine induced pluripotent stem cells.
Title: Bone morphogenetic protein 4 and retinoic acid trigger bovine VASA homolog expression in differentiating bovine induced pluripotent stem cells.

The expression of VASA in bovine fetuses is reported.
Title: Identification, localization, and sequencing of fetal bovine VASA homolog.

VASA was highly represented in the testis and the ovary, and still present in the oocyte from antral follicles.
Title: Spatio-temporal expression of the germ cell marker genes MATER, ZAR1, GDF9, BMP15,andVASA in adult bovine tissues, oocytes, and preimplantation embryos.

The following DDX4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DDX4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa00146	NM_001278644.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01123	NM_001278643.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa41576	NM_001007819.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa00146	XM_024981231.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa01123	XM_024981230.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa45840	XM_024981227.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OBa45841	XM_024981229.1 Latest version!	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DDX4 ( NM_001278644.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001265573.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OBa00146
Clone ID Related Accession (Same CDS sequence)	XM_024981231.1 , NM_001278644.1
Accession Version	NM_001278644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126699.1 and JX437189.1. Transcript Variant: This variant (3) lacks an exon and represents use of an alternate splice donor site, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC 
AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT 
TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA 
GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA 
CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT 
GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG 
ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA 
AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT 
GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT 
GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA 
GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT 
GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA 
GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265573.1
CDS	27..2066
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSDPGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001278644.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278644.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC 
AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT 
TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA 
GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA 
CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT 
GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG 
ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA 
AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT 
GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT 
GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA 
GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT 
GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA 
GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_001278643.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001265572.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OBa01123
Clone ID Related Accession (Same CDS sequence)	NM_001278643.1 , XM_024981230.1
Accession Version	NM_001278643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC126699.1 and JX437188.1. Transcript Variant: This variant (2) lacks an in-frame coding exon compared to variant 1. The encoded isoform (2) is shorter compared to isoform 1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT 
TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT 
GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC 
AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG 
TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT 
CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT 
AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT 
GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG 
AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG 
ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA 
GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA 
CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT 
AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA 
GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG 
CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG 
GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA 
GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG 
TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG 
AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT 
TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT 
TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT 
CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT 
GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG 
TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG 
GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA 
ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA 
GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG 
GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT 
GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT 
GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA 
CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT 
GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG 
AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG 
TCATGGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001265572.1
CDS	27..2138
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSDPGESNKRENTSTVGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001278643.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001278643.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT 
TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT 
GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC 
AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG 
TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT 
CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT 
AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT 
GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG 
AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG 
ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA 
GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA 
CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT 
AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA 
GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG 
CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG 
GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA 
GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG 
TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG 
AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT 
TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT 
TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT 
CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT 
GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG 
TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG 
GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA 
ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA 
GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG 
GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT 
GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT 
GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA 
CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT 
GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG 
AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG 
TCATGGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( NM_001007819.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001007820.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

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CloneID	OBa41576
Clone ID Related Accession (Same CDS sequence)	NM_001007819.1
Accession Version	NM_001007819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF541971.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AF541971.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145491 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT 
ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA 
GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT 
TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT 
GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC 
CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC 
TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA 
TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA 
CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT 
ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG 
ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT 
ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGACGT TCAGCAGACC 
ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA 
AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT 
TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA 
GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA 
TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA 
AAGAATTACC CCGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT 
AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007820.1
CDS	24..2213
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSEMGDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTVGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYPGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	NM_001007819.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007819.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT 
ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA 
GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT 
TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT 
GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC 
CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC 
TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA 
TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA 
CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT 
ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG 
ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AGCTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT 
ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGACGT TCAGCAGACC 
ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA 
AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT 
TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA 
GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA 
TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA 
AAGAATTACC CCGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT 
AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_024981231.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836999.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.

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CloneID	OBa00146
Clone ID Related Accession (Same CDS sequence)	XM_024981231.1 , NM_001278644.1
Accession Version	XM_024981231.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC 
AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT 
TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA 
GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA 
CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT 
GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG 
ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA 
AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT 
GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT 
GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA 
GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT 
GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA 
GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836999.1
CDS	222..2261
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSDPGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_024981231.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981231.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TTTTTCAAAT AACAAGTTTG AAGAAGGTGA TAGCTCTGGT 
TTCTGGAGAG AATCTAGTAT TGACTGTGAA GATAATCAAA CACGGAACAG AGGGTTTTCC 
AAGAGAGGCG GTTATCAAGA CGGAAATGAT TCAGAAGCTT TAGGATCGTC CAGAAGAGGT 
GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA GGATTTGGTC GAGGAAGTCC AAATAGTGAT 
TATGAACAAG ATGAGGGGAC CCAGCGCTCT GGTGGCATTT TTGGTTCTAG AAGATCAGCA 
TTAAGTGGTG CAGGTAATGG TGACACTTTC CAAAGCAGAA GTGGTGGTGG AAGTGGACGA 
GGTGGTTACA AAGGTTTAAA TGAAGAAGTA ATTACAGGCT CTGGAAAGAA TTCTTGGAAG 
TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC ACTCAAGGAC CAAAAGTGAC CTACATACCA 
CCTCCTCCAC CTGAAGATGA GGACTCCATC TTTGCACATT ATCAAACAGG CATAAACTTT 
GACAAATACG ATACTATTCT TGTAGAAGTA TCTGGACATG ATCCACCACC AGCAATTCTG 
ACTTTTGAAG AAGCTAATCT TTGTCAGACA CTGAATAACA ACATTGCTAA AGCTGGTTAT 
ACTAAGCTTA CTCCTGTGCA AAAATACAGT ATTCCTATTA TACAAGGAGG ACGAGATTTG 
ATGGCTTGTG CTCAGACGGG CTCTGGAAAG ACTGCAGCTT TTCTCTTGCC AATTTTGGCT 
CATATGATGC GTGATGGAAT AACTGCCAGT CGTTTTAAAG AACTGCAGGA ACCAGAGTGT 
ATTATTGTAG CACCAACTCG AGAGTTGATC AATCAGATCT ATTTGGAAGC CAGAAAATTT 
TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC ATATATGGGG GAACCCAGTT GGGGCATTCA 
ATTCGACAAA TAGTACAAGG CTGTAATATA TTATGTGCTA CTCCTGGGAG ACTGATGGAT 
GTCATAGGCA AAGAAAAGAT TGGTCTCAGA CAAGTCAAAT ACTTAGTTTT GGATGAAGCT 
GATCGCATGC TGGATATGGG TTTTGGACCA GAGATGAAAA AGTTAATTTC CTGCCCAGGA 
ATGCCATCAA AGGAACAGCG TCAGACCCTT ATGTTCAGTG CAACTTTTCC AGAAGAAATT 
CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG AATTATTTGT TTGTTGCTGT TGGACAAGTG 
GGTGGAGCAT GTAGAGATGT TCAGCAGACC ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA 
GAAAAACTTG TTGAAATTCT ACGAAACATA GGTGATGAAA GAACTATGGT CTTTGTTGAA 
ACTAAGAAAA AAGCAGATTT TATTGCCACT TTTCTTTGTC AAGAAAAAAT ATCAACAACA 
AGTATTCATG GTGATCGTGA ACAGAGAGAA AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT 
GGAAAGTGCC CTGTTCTTGT TGCTACTTCA GTAGCTGCCC GAGGGCTGGA TATTGAAAAT 
GTTCAGCATG TTATTAATTT TGATCTTCCT TCTACCATTG ATGAATATGT TCATCGAATT 
GGGCGTACTG GTCGTTGTGG AAATACTGGC AGAGCTATTT CCTTTTTTGA TCTGGAATCA 
GATAGCCAGT TAGCACAGCC TCTAGTGAAA GTGCTATCAG ATGCTCAACA GGATGTTCCT 
GCATGGTTAG AAGAAATTGC CTTTAGTACA TATGGTCCTG GCTTCAGTGG TAATGCAAGA 
GGAAATGTGT TTGCATCAGT CGATACTAGA AAGAATTACC ACGGCAAGAG CTCTTTGAAC 
ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_024981230.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836998.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa01123
Clone ID Related Accession (Same CDS sequence)	NM_001278643.1 , XM_024981230.1
Accession Version	XM_024981230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2112bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT 
TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT 
GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC 
AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG 
TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT 
CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT 
AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT 
GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG 
AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG 
ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA 
GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA 
CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT 
AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA 
GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG 
CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG 
GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA 
GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG 
TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG 
AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT 
TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT 
TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT 
CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT 
GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG 
TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG 
GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA 
ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA 
GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG 
GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT 
GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT 
GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA 
CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT 
GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG 
AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG 
TCATGGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836998.1
CDS	223..2334
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSDPGESNKRENTSTVGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_024981230.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981230.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGATCCTGG TGAGTCTAAT AAAAGAGAGA ATACGTCCAC AGTGGGTGGT 
TTTGGAGTTG GAAAGAGTTT TGGAAACAGA GGTTTTTCAA ATAACAAGTT TGAAGAAGGT 
GATAGCTCTG GTTTCTGGAG AGAATCTAGT ATTGACTGTG AAGATAATCA AACACGGAAC 
AGAGGGTTTT CCAAGAGAGG CGGTTATCAA GACGGAAATG ATTCAGAAGC TTTAGGATCG 
TCCAGAAGAG GTGGAAGAGG TAGTTTCCGA GGTTGCCGTG GAGGATTTGG TCGAGGAAGT 
CCAAATAGTG ATTATGAACA AGATGAGGGG ACCCAGCGCT CTGGTGGCAT TTTTGGTTCT 
AGAAGATCAG CATTAAGTGG TGCAGGTAAT GGTGACACTT TCCAAAGCAG AAGTGGTGGT 
GGAAGTGGAC GAGGTGGTTA CAAAGGTTTA AATGAAGAAG TAATTACAGG CTCTGGAAAG 
AATTCTTGGA AGTCAGAAGC TGAAGGAGGA GAAAGTGGTG ACACTCAAGG ACCAAAAGTG 
ACCTACATAC CACCTCCTCC ACCTGAAGAT GAGGACTCCA TCTTTGCACA TTATCAAACA 
GGCATAAACT TTGACAAATA CGATACTATT CTTGTAGAAG TATCTGGACA TGATCCACCA 
CCAGCAATTC TGACTTTTGA AGAAGCTAAT CTTTGTCAGA CACTGAATAA CAACATTGCT 
AAAGCTGGTT ATACTAAGCT TACTCCTGTG CAAAAATACA GTATTCCTAT TATACAAGGA 
GGACGAGATT TGATGGCTTG TGCTCAGACG GGCTCTGGAA AGACTGCAGC TTTTCTCTTG 
CCAATTTTGG CTCATATGAT GCGTGATGGA ATAACTGCCA GTCGTTTTAA AGAACTGCAG 
GAACCAGAGT GTATTATTGT AGCACCAACT CGAGAGTTGA TCAATCAGAT CTATTTGGAA 
GCCAGAAAAT TTTCTTTTGG GACTTGTGTA AGAGCAGTTG TCATATATGG GGGAACCCAG 
TTGGGGCATT CAATTCGACA AATAGTACAA GGCTGTAATA TATTATGTGC TACTCCTGGG 
AGACTGATGG ATGTCATAGG CAAAGAAAAG ATTGGTCTCA GACAAGTCAA ATACTTAGTT 
TTGGATGAAG CTGATCGCAT GCTGGATATG GGTTTTGGAC CAGAGATGAA AAAGTTAATT 
TCCTGCCCAG GAATGCCATC AAAGGAACAG CGTCAGACCC TTATGTTCAG TGCAACTTTT 
CCAGAAGAAA TTCAAAGGTT GGCTGGGGAG TTTTTAAAGT CGAATTATTT GTTTGTTGCT 
GTTGGACAAG TGGGTGGAGC ATGTAGAGAT GTTCAGCAGA CCATTCTTCA AGTTGGCCAG 
TACTCAAAAA GAGAAAAACT TGTTGAAATT CTACGAAACA TAGGTGATGA AAGAACTATG 
GTCTTTGTTG AAACTAAGAA AAAAGCAGAT TTTATTGCCA CTTTTCTTTG TCAAGAAAAA 
ATATCAACAA CAAGTATTCA TGGTGATCGT GAACAGAGAG AAAGAGAGCA AGCTCTTGGA 
GATTTCCGCT GTGGAAAGTG CCCTGTTCTT GTTGCTACTT CAGTAGCTGC CCGAGGGCTG 
GATATTGAAA ATGTTCAGCA TGTTATTAAT TTTGATCTTC CTTCTACCAT TGATGAATAT 
GTTCATCGAA TTGGGCGTAC TGGTCGTTGT GGAAATACTG GCAGAGCTAT TTCCTTTTTT 
GATCTGGAAT CAGATAGCCA GTTAGCACAG CCTCTAGTGA AAGTGCTATC AGATGCTCAA 
CAGGATGTTC CTGCATGGTT AGAAGAAATT GCCTTTAGTA CATATGGTCC TGGCTTCAGT 
GGTAATGCAA GAGGAAATGT GTTTGCATCA GTCGATACTA GAAAGAATTA CCACGGCAAG 
AGCTCTTTGA ACACAGCAGG GTTTTCTTCA ACACAAGCTC CTAATCCAGT AGACGATGAG 
TCATGGGATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_024981227.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836995.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa45840
Clone ID Related Accession (Same CDS sequence)	XM_024981227.1
Accession Version	XM_024981227.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT 
ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA 
GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT 
TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT 
GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC 
CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC 
TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA 
TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA 
CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT 
ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG 
ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AACTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT 
ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA 
AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT 
TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA 
GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA 
TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA 
AAGAATTACC ACGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT 
AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836995.1
CDS	224..2413
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSEMGDGSSRRDHFMRSGFASGRSLGNRDPGESNKRENTSTVGGFGVGKSFGNRGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_024981227.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981227.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTG AGTCTAATAA AAGAGAGAAT 
ACGTCCACAG TGGGTGGTTT TGGAGTTGGA AAGAGTTTTG GAAACAGAGG TTTTTCAAAT 
AACAAGTTTG AAGAAGGTGA TAGCTCTGGT TTCTGGAGAG AATCTAGTAT TGACTGTGAA 
GATAATCAAA CACGGAACAG AGGGTTTTCC AAGAGAGGCG GTTATCAAGA CGGAAATGAT 
TCAGAAGCTT TAGGATCGTC CAGAAGAGGT GGAAGAGGTA GTTTCCGAGG TTGCCGTGGA 
GGATTTGGTC GAGGAAGTCC AAATAGTGAT TATGAACAAG ATGAGGGGAC CCAGCGCTCT 
GGTGGCATTT TTGGTTCTAG AAGATCAGCA TTAAGTGGTG CAGGTAATGG TGACACTTTC 
CAAAGCAGAA GTGGTGGTGG AAGTGGACGA GGTGGTTACA AAGGTTTAAA TGAAGAAGTA 
ATTACAGGCT CTGGAAAGAA TTCTTGGAAG TCAGAAGCTG AAGGAGGAGA AAGTGGTGAC 
ACTCAAGGAC CAAAAGTGAC CTACATACCA CCTCCTCCAC CTGAAGATGA GGACTCCATC 
TTTGCACATT ATCAAACAGG CATAAACTTT GACAAATACG ATACTATTCT TGTAGAAGTA 
TCTGGACATG ATCCACCACC AGCAATTCTG ACTTTTGAAG AAGCTAATCT TTGTCAGACA 
CTGAATAACA ACATTGCTAA AGCTGGTTAT ACTAAGCTTA CTCCTGTGCA AAAATACAGT 
ATTCCTATTA TACAAGGAGG ACGAGATTTG ATGGCTTGTG CTCAGACGGG CTCTGGAAAG 
ACTGCAGCTT TTCTCTTGCC AATTTTGGCT CATATGATGC GTGATGGAAT AACTGCCAGT 
CGTTTTAAAG AACTGCAGGA ACCAGAGTGT ATTATTGTAG CACCAACTCG AGAGTTGATC 
AATCAGATCT ATTTGGAAGC CAGAAAATTT TCTTTTGGGA CTTGTGTAAG AGCAGTTGTC 
ATATATGGGG GAACCCAGTT GGGGCATTCA ATTCGACAAA TAGTACAAGG CTGTAATATA 
TTATGTGCTA CTCCTGGGAG ACTGATGGAT GTCATAGGCA AAGAAAAGAT TGGTCTCAGA 
CAAGTCAAAT ACTTAGTTTT GGATGAAGCT GATCGCATGC TGGATATGGG TTTTGGACCA 
GAGATGAAAA AGTTAATTTC CTGCCCAGGA ATGCCATCAA AGGAACAGCG TCAGACCCTT 
ATGTTCAGTG CAACTTTTCC AGAAGAAATT CAAAGGTTGG CTGGGGAGTT TTTAAAGTCG 
AATTATTTGT TTGTTGCTGT TGGACAAGTG GGTGGAGCAT GTAGAGATGT TCAGCAGACC 
ATTCTTCAAG TTGGCCAGTA CTCAAAAAGA GAAAAACTTG TTGAAATTCT ACGAAACATA 
GGTGATGAAA GAACTATGGT CTTTGTTGAA ACTAAGAAAA AAGCAGATTT TATTGCCACT 
TTTCTTTGTC AAGAAAAAAT ATCAACAACA AGTATTCATG GTGATCGTGA ACAGAGAGAA 
AGAGAGCAAG CTCTTGGAGA TTTCCGCTGT GGAAAGTGCC CTGTTCTTGT TGCTACTTCA 
GTAGCTGCCC GAGGGCTGGA TATTGAAAAT GTTCAGCATG TTATTAATTT TGATCTTCCT 
TCTACCATTG ATGAATATGT TCATCGAATT GGGCGTACTG GTCGTTGTGG AAATACTGGC 
AGAGCTATTT CCTTTTTTGA TCTGGAATCA GATAGCCAGT TAGCACAGCC TCTAGTGAAA 
GTGCTATCAG ATGCTCAACA GGATGTTCCT GCATGGTTAG AAGAAATTGC CTTTAGTACA 
TATGGTCCTG GCTTCAGTGG TAATGCAAGA GGAAATGTGT TTGCATCAGT CGATACTAGA 
AAGAATTACC ACGGCAAGAG CTCTTTGAAC ACAGCAGGGT TTTCTTCAAC ACAAGCTCCT 
AATCCAGTAG ACGATGAGTC ATGGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DDX4 ( XM_024981229.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024836997.1 Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa45841
Clone ID Related Accession (Same CDS sequence)	XM_024981229.1
Accession Version	XM_024981229.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	probable ATP-dependent RNA helicase DDX4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037347.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTT TTTCAAATAA CAAGTTTGAA 
GAAGGTGATA GCTCTGGTTT CTGGAGAGAA TCTAGTATTG ACTGTGAAGA TAATCAAACA 
CGGAACAGAG GGTTTTCCAA GAGAGGCGGT TATCAAGACG GAAATGATTC AGAAGCTTTA 
GGATCGTCCA GAAGAGGTGG AAGAGGTAGT TTCCGAGGTT GCCGTGGAGG ATTTGGTCGA 
GGAAGTCCAA ATAGTGATTA TGAACAAGAT GAGGGGACCC AGCGCTCTGG TGGCATTTTT 
GGTTCTAGAA GATCAGCATT AAGTGGTGCA GGTAATGGTG ACACTTTCCA AAGCAGAAGT 
GGTGGTGGAA GTGGACGAGG TGGTTACAAA GGTTTAAATG AAGAAGTAAT TACAGGCTCT 
GGAAAGAATT CTTGGAAGTC AGAAGCTGAA GGAGGAGAAA GTGGTGACAC TCAAGGACCA 
AAAGTGACCT ACATACCACC TCCTCCACCT GAAGATGAGG ACTCCATCTT TGCACATTAT 
CAAACAGGCA TAAACTTTGA CAAATACGAT ACTATTCTTG TAGAAGTATC TGGACATGAT 
CCACCACCAG CAATTCTGAC TTTTGAAGAA GCTAATCTTT GTCAGACACT GAATAACAAC 
ATTGCTAAAG CTGGTTATAC TAAGCTTACT CCTGTGCAAA AATACAGTAT TCCTATTATA 
CAAGGAGGAC GAGATTTGAT GGCTTGTGCT CAGACGGGCT CTGGAAAGAC TGCAGCTTTT 
CTCTTGCCAA TTTTGGCTCA TATGATGCGT GATGGAATAA CTGCCAGTCG TTTTAAAGAA 
CTGCAGGAAC CAGAGTGTAT TATTGTAGCA CCAACTCGAG AGTTGATCAA TCAGATCTAT 
TTGGAAGCCA GAAAATTTTC TTTTGGGACT TGTGTAAGAG CAGTTGTCAT ATATGGGGGA 
ACCCAGTTGG GGCATTCAAT TCGACAAATA GTACAAGGCT GTAATATATT ATGTGCTACT 
CCTGGGAGAC TGATGGATGT CATAGGCAAA GAAAAGATTG GTCTCAGACA AGTCAAATAC 
TTAGTTTTGG ATGAAGCTGA TCGCATGCTG GATATGGGTT TTGGACCAGA GATGAAAAAG 
TTAATTTCCT GCCCAGGAAT GCCATCAAAG GAACAGCGTC AGACCCTTAT GTTCAGTGCA 
ACTTTTCCAG AAGAAATTCA AAGGTTGGCT GGGGAGTTTT TAAAGTCGAA TTATTTGTTT 
GTTGCTGTTG GACAAGTGGG TGGAGCATGT AGAGATGTTC AGCAGACCAT TCTTCAAGTT 
GGCCAGTACT CAAAAAGAGA AAAACTTGTT GAAATTCTAC GAAACATAGG TGATGAAAGA 
ACTATGGTCT TTGTTGAAAC TAAGAAAAAA GCAGATTTTA TTGCCACTTT TCTTTGTCAA 
GAAAAAATAT CAACAACAAG TATTCATGGT GATCGTGAAC AGAGAGAAAG AGAGCAAGCT 
CTTGGAGATT TCCGCTGTGG AAAGTGCCCT GTTCTTGTTG CTACTTCAGT AGCTGCCCGA 
GGGCTGGATA TTGAAAATGT TCAGCATGTT ATTAATTTTG ATCTTCCTTC TACCATTGAT 
GAATATGTTC ATCGAATTGG GCGTACTGGT CGTTGTGGAA ATACTGGCAG AGCTATTTCC 
TTTTTTGATC TGGAATCAGA TAGCCAGTTA GCACAGCCTC TAGTGAAAGT GCTATCAGAT 
GCTCAACAGG ATGTTCCTGC ATGGTTAGAA GAAATTGCCT TTAGTACATA TGGTCCTGGC 
TTCAGTGGTA ATGCAAGAGG AAATGTGTTT GCATCAGTCG ATACTAGAAA GAATTACCAC 
GGCAAGAGCT CTTTGAACAC AGCAGGGTTT TCTTCAACAC AAGCTCCTAA TCCAGTAGAC 
GATGAGTCAT GGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024836997.1
CDS	150..2267
Translation	MGDEDWEAEIIKPHISSYVPVFEKDRYSSGANGDTFNRTPASSSEMGDGSSRRDHFMRSGFASGRSLGNRDPGFSNNKFEEGDSSGFWRESSIDCEDNQTRNRGFSKRGGYQDGNDSEALGSSRRGGRGSFRGCRGGFGRGSPNSDYEQDEGTQRSGGIFGSRRSALSGAGNGDTFQSRSGGGSGRGGYKGLNEEVITGSGKNSWKSEAEGGESGDTQGPKVTYIPPPPPEDEDSIFAHYQTGINFDKYDTILVEVSGHDPPPAILTFEEANLCQTLNNNIAKAGYTKLTPVQKYSIPIIQGGRDLMACAQTGSGKTAAFLLPILAHMMRDGITASRFKELQEPECIIVAPTRELINQIYLEARKFSFGTCVRAVVIYGGTQLGHSIRQIVQGCNILCATPGRLMDVIGKEKIGLRQVKYLVLDEADRMLDMGFGPEMKKLISCPGMPSKEQRQTLMFSATFPEEIQRLAGEFLKSNYLFVAVGQVGGACRDVQQTILQVGQYSKREKLVEILRNIGDERTMVFVETKKKADFIATFLCQEKISTTSIHGDREQREREQALGDFRCGKCPVLVATSVAARGLDIENVQHVINFDLPSTIDEYVHRIGRTGRCGNTGRAISFFDLESDSQLAQPLVKVLSDAQQDVPAWLEEIAFSTYGPGFSGNARGNVFASVDTRKNYHGKSSLNTAGFSSTQAPNPVDDESWD

Target ORF information:

RefSeq Version	XM_024981229.1
Organism	Bos taurus(cattle)
Definition	Bos taurus DEAD-box helicase 4 (DDX4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024981229.1

ORF Insert Sequence:

ATGGGAGATG AAGATTGGGA AGCAGAAATA ATCAAACCTC ATATATCTTC CTATGTTCCT 
GTATTTGAGA AGGATAGGTA TTCTTCTGGA GCAAATGGAG ACACTTTTAA CAGGACTCCG 
GCTTCATCGT CAGAAATGGG TGATGGATCA TCTCGAAGAG ATCATTTCAT GAGAAGTGGA 
TTTGCCTCTG GGAGGAGTTT GGGAAACAGA GATCCTGGTT TTTCAAATAA CAAGTTTGAA 
GAAGGTGATA GCTCTGGTTT CTGGAGAGAA TCTAGTATTG ACTGTGAAGA TAATCAAACA 
CGGAACAGAG GGTTTTCCAA GAGAGGCGGT TATCAAGACG GAAATGATTC AGAAGCTTTA 
GGATCGTCCA GAAGAGGTGG AAGAGGTAGT TTCCGAGGTT GCCGTGGAGG ATTTGGTCGA 
GGAAGTCCAA ATAGTGATTA TGAACAAGAT GAGGGGACCC AGCGCTCTGG TGGCATTTTT 
GGTTCTAGAA GATCAGCATT AAGTGGTGCA GGTAATGGTG ACACTTTCCA AAGCAGAAGT 
GGTGGTGGAA GTGGACGAGG TGGTTACAAA GGTTTAAATG AAGAAGTAAT TACAGGCTCT 
GGAAAGAATT CTTGGAAGTC AGAAGCTGAA GGAGGAGAAA GTGGTGACAC TCAAGGACCA 
AAAGTGACCT ACATACCACC TCCTCCACCT GAAGATGAGG ACTCCATCTT TGCACATTAT 
CAAACAGGCA TAAACTTTGA CAAATACGAT ACTATTCTTG TAGAAGTATC TGGACATGAT 
CCACCACCAG CAATTCTGAC TTTTGAAGAA GCTAATCTTT GTCAGACACT GAATAACAAC 
ATTGCTAAAG CTGGTTATAC TAAGCTTACT CCTGTGCAAA AATACAGTAT TCCTATTATA 
CAAGGAGGAC GAGATTTGAT GGCTTGTGCT CAGACGGGCT CTGGAAAGAC TGCAGCTTTT 
CTCTTGCCAA TTTTGGCTCA TATGATGCGT GATGGAATAA CTGCCAGTCG TTTTAAAGAA 
CTGCAGGAAC CAGAGTGTAT TATTGTAGCA CCAACTCGAG AGTTGATCAA TCAGATCTAT 
TTGGAAGCCA GAAAATTTTC TTTTGGGACT TGTGTAAGAG CAGTTGTCAT ATATGGGGGA 
ACCCAGTTGG GGCATTCAAT TCGACAAATA GTACAAGGCT GTAATATATT ATGTGCTACT 
CCTGGGAGAC TGATGGATGT CATAGGCAAA GAAAAGATTG GTCTCAGACA AGTCAAATAC 
TTAGTTTTGG ATGAAGCTGA TCGCATGCTG GATATGGGTT TTGGACCAGA GATGAAAAAG 
TTAATTTCCT GCCCAGGAAT GCCATCAAAG GAACAGCGTC AGACCCTTAT GTTCAGTGCA 
ACTTTTCCAG AAGAAATTCA AAGGTTGGCT GGGGAGTTTT TAAAGTCGAA TTATTTGTTT 
GTTGCTGTTG GACAAGTGGG TGGAGCATGT AGAGATGTTC AGCAGACCAT TCTTCAAGTT 
GGCCAGTACT CAAAAAGAGA AAAACTTGTT GAAATTCTAC GAAACATAGG TGATGAAAGA 
ACTATGGTCT TTGTTGAAAC TAAGAAAAAA GCAGATTTTA TTGCCACTTT TCTTTGTCAA 
GAAAAAATAT CAACAACAAG TATTCATGGT GATCGTGAAC AGAGAGAAAG AGAGCAAGCT 
CTTGGAGATT TCCGCTGTGG AAAGTGCCCT GTTCTTGTTG CTACTTCAGT AGCTGCCCGA 
GGGCTGGATA TTGAAAATGT TCAGCATGTT ATTAATTTTG ATCTTCCTTC TACCATTGAT 
GAATATGTTC ATCGAATTGG GCGTACTGGT CGTTGTGGAA ATACTGGCAG AGCTATTTCC 
TTTTTTGATC TGGAATCAGA TAGCCAGTTA GCACAGCCTC TAGTGAAAGT GCTATCAGAT 
GCTCAACAGG ATGTTCCTGC ATGGTTAGAA GAAATTGCCT TTAGTACATA TGGTCCTGGC 
TTCAGTGGTA ATGCAAGAGG AAATGTGTTT GCATCAGTCG ATACTAGAAA GAATTACCAC 
GGCAAGAGCT CTTTGAACAC AGCAGGGTTT TCTTCAACAC AAGCTCCTAA TCCAGTAGAC 
GATGAGTCAT GGGATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bone morphogenetic protein 4 and retinoic acid trigger bovine VASA homolog expression in differentiating bovine induced pluripotent stem cells.
Molecular reproduction and development83(2)149-61(2016 Feb)
Malaver-Ortega LF,Sumer H,Jain K,Verma PJ

Comparison of the efficacy of three concentrations of retinoic acid for transdifferentiation induction in sheep marrow-derived mesenchymal stem cells into male germ cells.
Andrologia46(1)24-35(2014 Feb)
Ghasemzadeh-Hasankolaei M,Eslaminejad MB,Batavani R,Sedighi-Gilani M

Splice variants and promoter methylation status of the Bovine Vasa Homology (Bvh) gene may be involved in bull spermatogenesis.
BMC genetics1458(2013 Jul)
Luo H,Zhou Y,Li Y,Li Q

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Identification, localization, and sequencing of fetal bovine VASA homolog.
Animal reproduction science101(3-4)241-51(2007 Oct)
Bartholomew RA,Parks JE

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DDX4 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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