INSR cDNA ORF clone, Bos taurus(cattle)

The following INSR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INSR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa21067 XM_002688832.6
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21067 XM_015464089.1
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21068 XM_015464090.1
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21068 XM_005208815.4
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21069 XM_015464091.1
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21069 XM_005208816.4
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21070 XM_015464092.1
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21070 XM_005208817.4
Latest version!
Bos taurus insulin receptor (INSR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OBa21067 XM_002688832.5 Bos taurus insulin receptor (INSR), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21068 XM_005208815.3 Bos taurus insulin receptor (INSR), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21070 XM_005208817.3 Bos taurus insulin receptor (INSR), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OBa21069 XM_005208816.3 Bos taurus insulin receptor (INSR), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa21067
    Clone ID Related Accession (Same CDS sequence) XM_002688832.6 , XM_002688832.5 , XM_015464089.1
    Accession Version XM_002688832.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002688832.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002688878.3
    CDS489..4637
    Translation

    Target ORF information:

    RefSeq Version XM_002688832.6
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688832.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21067
    Clone ID Related Accession (Same CDS sequence) XM_002688832.6 , XM_002688832.5 , XM_015464089.1
    Accession Version XM_002688832.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002688832.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002688878.3
    CDS1..4149
    Translation

    Target ORF information:

    RefSeq Version XM_002688832.5
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002688832.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21067
    Clone ID Related Accession (Same CDS sequence) XM_002688832.6 , XM_002688832.5 , XM_015464089.1
    Accession Version XM_015464089.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4149bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319575.1
    CDS1..4149
    Translation

    Target ORF information:

    RefSeq Version XM_015464089.1
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464089.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTTAGACGTG CCATCAAATA TTGCAAAAAT CATCATCGGT
    CCCCTCATCT TTGTCTTTCT CTTCAGTGTT GTGATTGGAA GTATTTGTCT ATTCCTGAGA
    AAGAGGCAGC CAGATGGGCC ATTGGGACCA CTGTATGCTT CTTCAAACCC AGAGTACCTC
    AGTGCCAGCG ATGTGTTTCC ATGCTCTGTG TATGTGCCGG ACGAGTGGGA GGTGCCTCGA
    GAGAAGATCA CACTCCTGCG AGAATTGGGG CAAGGCTCCT TTGGCATGGT GTACGAGGGC
    AACGCCAGGG ACATTGTCAA GGGTGAGGCG GAGACGCGCG TGGCAGTGAA GACGGTCAAC
    GAGTCAGCCA GCCTGCGAGA GCGGATCGAG TTTCTCAACG AGGCTTCGGT CATGAAGGGC
    TTCACCTGCC ACCATGTGGT CCGCCTCCTG GGGGTGGTGT CCAAAGGCCA GCCAACGCTG
    GTGGTGATGG AGTTGATGGC TCACGGAGAC CTGAAGAGTT ACCTCCGTTC CCTGCGGCCT
    GAGGCTGAGA ATAACCCCGG CCGCCCTCCG CCTACCCTGC AAGAAATGAT TCAGATGGCG
    GCAGAGATCG CCGATGGGAT GGCATACCTG AACGCTAAGA AGTTCGTGCA CCGGGATCTT
    GCAGCCCGGA ACTGCATGGT TGCCCACGAC TTTACAGTCA AAATTGGAGA CTTTGGAATG
    ACAAGAGACA TCTATGAAAC GGATTACTAC CGGAAAGGGG GCAAAGGTCT GCTCCCCGTG
    CGGTGGATGG CACCTGAATC CCTGAAGGAC GGAGTCTTCA CCACCTCTTC AGACATGTGG
    TCTTTTGGTG TGGTCCTTTG GGAAATTACC AGCTTGGCAG AACAGCCTTA CCAAGGTCTA
    TCAAATGAGC AGGTGCTGAA ATTTGTCATG GATGGAGGGT ACCTGGATCA ACCTGACAAT
    TGTCCAGAGA GAGTCACCGA CCTGATGCGG ATGTGCTGGC AGTTCAACCC CAAGATGCGT
    CCAACCTTCC TGGAGATCGT GGACCTGCTC AAGGACGACC TGCACCCCAG CTTTCCCGAA
    GTGTCCTTCT TCCACAGCGA GGAGAACAAG GCGCCCGAGA GCGAGGAGCT GGAAATGGAG
    TTTGAGGACA TGGAGAGCGT GCCCCTGGAC CGGGCCTCGC ACGCGCAGAG AGAGGAGGCC
    GGGGGCCGGG ATGGAGGGTC GGCGCTGGGG CTAAAGCGAA ACTATGACGA GCACATCCCC
    TACACCCACA TGAACGGGGG CAAGAAAAAC GGACGGATTC TGACTTTGCC CCGGTCAAAT
    CCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21068
    Clone ID Related Accession (Same CDS sequence) XM_015464090.1 , XM_005208815.4 , XM_005208815.3
    Accession Version XM_015464090.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319576.1
    CDS167..4312
    Translation

    Target ORF information:

    RefSeq Version XM_015464090.1
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464090.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21068
    Clone ID Related Accession (Same CDS sequence) XM_015464090.1 , XM_005208815.4 , XM_005208815.3
    Accession Version XM_005208815.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208815.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208872.1
    CDS29..4174
    Translation

    Target ORF information:

    RefSeq Version XM_005208815.4
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208815.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21068
    Clone ID Related Accession (Same CDS sequence) XM_015464090.1 , XM_005208815.4 , XM_005208815.3
    Accession Version XM_005208815.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4146bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208815.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208872.1
    CDS167..4312
    Translation

    Target ORF information:

    RefSeq Version XM_005208815.3
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208815.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GAAAATCATC TTCAGGCCCT GTTGCCGAGG ACGCTAGGCC TTCGAGGAAA
    CGCAGAGCCC TTGGTGATGT GGGGAATGTG ACAGCAGCCG TGCCCACTGC TCTGGGGCTC
    CCCAATACCT CCTCCACCAG CACGCCCATG AGCTCGGAGG AGCACAGACC CTTCGAGAAA
    GTGGTGAACA AGGAGTCGCT GGTCATCTCC GGCCTGCGTC ACTTTACTGG CTATCGCATC
    GAGCTTCAGG CTTGCAACCA AGATTCCCCC GAGGAGAGGT GTAGCGTGGC TGCCTATGTC
    AGCGCCAGGA CCATGCCTGA AGCCAAGGCA GATGATATTG TCGGCCCCGT GACCCATGAA
    ATCTTCGAGA ACAACGTTGT TCACTTGATG TGGCAGGAGC CCAAGGAACC CAACGGTCTG
    ATTGTGCTGT ATGAAGTGAG TTACCGACGA TATGGCGAGG AGGAGCTGCA CCTCTGCGTC
    TCCCGCCGAC ATTATGCTCT GGAGCGTGGC TGCCGGCTGC GTGGGCTGCT GCCTGGGAAT
    TACAGTGTGC GAGTTCGGGC CACCTCCCTG GCAGGCAATG GGTCTTGGAC GGAAGCCACC
    TATTTCTACG TGACAGACTA TTACGTGCCA TCAAATATTG CAAAAATCAT CATCGGTCCC
    CTCATCTTTG TCTTTCTCTT CAGTGTTGTG ATTGGAAGTA TTTGTCTATT CCTGAGAAAG
    AGGCAGCCAG ATGGGCCATT GGGACCACTG TATGCTTCTT CAAACCCAGA GTACCTCAGT
    GCCAGCGATG TGTTTCCATG CTCTGTGTAT GTGCCGGACG AGTGGGAGGT GCCTCGAGAG
    AAGATCACAC TCCTGCGAGA ATTGGGGCAA GGCTCCTTTG GCATGGTGTA CGAGGGCAAC
    GCCAGGGACA TTGTCAAGGG TGAGGCGGAG ACGCGCGTGG CAGTGAAGAC GGTCAACGAG
    TCAGCCAGCC TGCGAGAGCG GATCGAGTTT CTCAACGAGG CTTCGGTCAT GAAGGGCTTC
    ACCTGCCACC ATGTGGTCCG CCTCCTGGGG GTGGTGTCCA AAGGCCAGCC AACGCTGGTG
    GTGATGGAGT TGATGGCTCA CGGAGACCTG AAGAGTTACC TCCGTTCCCT GCGGCCTGAG
    GCTGAGAATA ACCCCGGCCG CCCTCCGCCT ACCCTGCAAG AAATGATTCA GATGGCGGCA
    GAGATCGCCG ATGGGATGGC ATACCTGAAC GCTAAGAAGT TCGTGCACCG GGATCTTGCA
    GCCCGGAACT GCATGGTTGC CCACGACTTT ACAGTCAAAA TTGGAGACTT TGGAATGACA
    AGAGACATCT ATGAAACGGA TTACTACCGG AAAGGGGGCA AAGGTCTGCT CCCCGTGCGG
    TGGATGGCAC CTGAATCCCT GAAGGACGGA GTCTTCACCA CCTCTTCAGA CATGTGGTCT
    TTTGGTGTGG TCCTTTGGGA AATTACCAGC TTGGCAGAAC AGCCTTACCA AGGTCTATCA
    AATGAGCAGG TGCTGAAATT TGTCATGGAT GGAGGGTACC TGGATCAACC TGACAATTGT
    CCAGAGAGAG TCACCGACCT GATGCGGATG TGCTGGCAGT TCAACCCCAA GATGCGTCCA
    ACCTTCCTGG AGATCGTGGA CCTGCTCAAG GACGACCTGC ACCCCAGCTT TCCCGAAGTG
    TCCTTCTTCC ACAGCGAGGA GAACAAGGCG CCCGAGAGCG AGGAGCTGGA AATGGAGTTT
    GAGGACATGG AGAGCGTGCC CCTGGACCGG GCCTCGCACG CGCAGAGAGA GGAGGCCGGG
    GGCCGGGATG GAGGGTCGGC GCTGGGGCTA AAGCGAAACT ATGACGAGCA CATCCCCTAC
    ACCCACATGA ACGGGGGCAA GAAAAACGGA CGGATTCTGA CTTTGCCCCG GTCAAATCCT
    TCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21069
    Clone ID Related Accession (Same CDS sequence) XM_015464091.1 , XM_005208816.4 , XM_005208816.3
    Accession Version XM_015464091.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319577.1
    CDS1..4113
    Translation

    Target ORF information:

    RefSeq Version XM_015464091.1
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21069
    Clone ID Related Accession (Same CDS sequence) XM_015464091.1 , XM_005208816.4 , XM_005208816.3
    Accession Version XM_005208816.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208816.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208873.1
    CDS471..4583
    Translation

    Target ORF information:

    RefSeq Version XM_005208816.4
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208816.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21069
    Clone ID Related Accession (Same CDS sequence) XM_015464091.1 , XM_005208816.4 , XM_005208816.3
    Accession Version XM_005208816.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208816.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208873.1
    CDS1..4113
    Translation

    Target ORF information:

    RefSeq Version XM_005208816.3
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208816.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTTAGA CGTGCCATCA
    AATATTGCAA AAATCATCAT CGGTCCCCTC ATCTTTGTCT TTCTCTTCAG TGTTGTGATT
    GGAAGTATTT GTCTATTCCT GAGAAAGAGG CAGCCAGATG GGCCATTGGG ACCACTGTAT
    GCTTCTTCAA ACCCAGAGTA CCTCAGTGCC AGCGATGTGT TTCCATGCTC TGTGTATGTG
    CCGGACGAGT GGGAGGTGCC TCGAGAGAAG ATCACACTCC TGCGAGAATT GGGGCAAGGC
    TCCTTTGGCA TGGTGTACGA GGGCAACGCC AGGGACATTG TCAAGGGTGA GGCGGAGACG
    CGCGTGGCAG TGAAGACGGT CAACGAGTCA GCCAGCCTGC GAGAGCGGAT CGAGTTTCTC
    AACGAGGCTT CGGTCATGAA GGGCTTCACC TGCCACCATG TGGTCCGCCT CCTGGGGGTG
    GTGTCCAAAG GCCAGCCAAC GCTGGTGGTG ATGGAGTTGA TGGCTCACGG AGACCTGAAG
    AGTTACCTCC GTTCCCTGCG GCCTGAGGCT GAGAATAACC CCGGCCGCCC TCCGCCTACC
    CTGCAAGAAA TGATTCAGAT GGCGGCAGAG ATCGCCGATG GGATGGCATA CCTGAACGCT
    AAGAAGTTCG TGCACCGGGA TCTTGCAGCC CGGAACTGCA TGGTTGCCCA CGACTTTACA
    GTCAAAATTG GAGACTTTGG AATGACAAGA GACATCTATG AAACGGATTA CTACCGGAAA
    GGGGGCAAAG GTCTGCTCCC CGTGCGGTGG ATGGCACCTG AATCCCTGAA GGACGGAGTC
    TTCACCACCT CTTCAGACAT GTGGTCTTTT GGTGTGGTCC TTTGGGAAAT TACCAGCTTG
    GCAGAACAGC CTTACCAAGG TCTATCAAAT GAGCAGGTGC TGAAATTTGT CATGGATGGA
    GGGTACCTGG ATCAACCTGA CAATTGTCCA GAGAGAGTCA CCGACCTGAT GCGGATGTGC
    TGGCAGTTCA ACCCCAAGAT GCGTCCAACC TTCCTGGAGA TCGTGGACCT GCTCAAGGAC
    GACCTGCACC CCAGCTTTCC CGAAGTGTCC TTCTTCCACA GCGAGGAGAA CAAGGCGCCC
    GAGAGCGAGG AGCTGGAAAT GGAGTTTGAG GACATGGAGA GCGTGCCCCT GGACCGGGCC
    TCGCACGCGC AGAGAGAGGA GGCCGGGGGC CGGGATGGAG GGTCGGCGCT GGGGCTAAAG
    CGAAACTATG ACGAGCACAT CCCCTACACC CACATGAACG GGGGCAAGAA AAACGGACGG
    ATTCTGACTT TGCCCCGGTC AAATCCTTCC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21070
    Clone ID Related Accession (Same CDS sequence) XM_005208817.4 , XM_005208817.3 , XM_015464092.1
    Accession Version XM_005208817.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037334.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005208817.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208874.1
    CDS29..4138
    Translation

    Target ORF information:

    RefSeq Version XM_005208817.4
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208817.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21070
    Clone ID Related Accession (Same CDS sequence) XM_005208817.4 , XM_005208817.3 , XM_015464092.1
    Accession Version XM_005208817.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104015.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005208817.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005208874.1
    CDS167..4276
    Translation

    Target ORF information:

    RefSeq Version XM_005208817.3
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005208817.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa21070
    Clone ID Related Accession (Same CDS sequence) XM_005208817.4 , XM_005208817.3 , XM_015464092.1
    Accession Version XM_015464092.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4110bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product insulin receptor isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645040.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319578.1
    CDS167..4276
    Translation

    Target ORF information:

    RefSeq Version XM_015464092.1
    Organism Bos taurus(cattle)
    Definition Bos taurus insulin receptor (INSR), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015464092.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGGCGCCG GGGGCGGGAG GGGGGCGGCA GCCGTGCTGC TGATGGTGGT CGTCGCCGCG 
    CTGCTGGTGG GCGCCGCGGG CCACCTGTAC CCTGGAGAAG TGTGCCCCGG TATGGATATC
    CGGAATAATC TCACCCGGCT GCATGAGCTG GCCAACTGCT CAGTCATCGA AGGACATTTG
    CAGATTCTGT TGATGTTCAA AACGAGACCT GAGGACTTCC GAGACCTCAG TTTCCCCAAA
    CTCATCATGA TCACTGATTA CTTGCTGCTC TTCCGGGTCT ATGGGCTGGA GAGCCTGAAG
    GACTTGTTCC CCAACCTCAC AGTTATCCGA GGCTCACGCC TCTTCTTTAA TTATGCACTG
    GTCATCTTTG AGATGGTTCA CCTGAAGGAG CTTGGCCTCT ACAACCTGAT GAACATCACC
    CGGGGGTCTG TCCGCATTGA GAAGAACAAT GAACTCTGCT ACCTGGCCAC CATCGACTGG
    TCTCGCATCC TGGATTCCGT GGAGGATAAT TATATCGTGC TGAACAAAGA CGACAATGAG
    GAGTGTGGGG ACATTTGTCC AGGCACCGCC AAGGGCAAGA CCAACTGCCC TGCCACTGTC
    ATCAACGGGC AATTTGTGGA GCGGTGCTGG ACACACAGCC ACTGCCAGAA AGTGTGTCCG
    ACCATCTGCA AGTCACATGG CTGCACCTCT GAGGGTCTCT GCTGCCACAG CGAGTGCTTG
    GGCAACTGCT CAGAGCCCGA CGACCCCACC AAGTGTGTGG CCTGTCGCAA CTTCTACCTG
    GACGGCAGGT GTGTGGAGAC CTGCCCGCCC CCTTACTACC ACTTCCAAGA CTGGCGCTGC
    GTGAACTTCA GCTTCTGCCA GGACCTGCAC AACAAATGCA AGAACTCTCG GAGGCAAGGC
    TGCCACCAGT ATGTCATTCA CAATAACAAG TGCATCCCCG AGTGCCCCTC TGGCTACACG
    ATGAATTCCA GCAACTTGAT GTGTACCCCT TGCCTGGGCC CCTGTCCCAA GGTATGCCAC
    CTCCTGGAAG GCGAGAAGAC CATCGATTCG GTGACATCTG CCCAGGAGCT CCGAGGCTGC
    ACCATCATCA ACGGAAGCTT AATCATCAAC ATTCGAGGGG GCAACAACCT GGCAGCTGAG
    CTAGAGGCCA ACCTTGGACT CATTGAAGAA ATTTCAGGAT ATCTGAAAAT CCGCCGGTCC
    TATGCCCTGG TGTCACTTTC CTTCTTCCGG AAGTTACGTC TGATTCGAGG GGAGACCTTG
    GAAATTGGGA ACTACTCTTT CTACGCCTTG GACAACCAGA ACCTAAGGCA GCTGTGGGAC
    TGGAGCAAAC ACAACCTCAC CATCACTCAG GGGAAACTCT TCTTCCACTA TAACCCCAAA
    CTCTGCCTGT CGGAAATTCA CAAGATGGAG GAAGTTTCAG GAACCAAGGG GCGCCAGGAG
    AGAAATGACA TTGCTCTAAA GACCAATGGG GACCAGGCAT CCTGTGAAAA TGAATTACTT
    AAATTTTCTT ACATTCGGAC ATCTTACGAC AAGATCTTGC TAAAATGGGA GCCGTATTGG
    CCTCCTGACT TCCGAGACCT CCTGGGGTTC ATGTTGTTCT ACAAAGAGGC CCCTTACCAG
    AACGTGACAG AATTCGATGG GCAGGATGCC TGCGGCTCCA ACAGCTGGAC AGTGGTGGAT
    ATTGACCCGC CTACGAGGTC CAACGACCCC AAGTCACAGA ACCACCCTGG GTGGCTGATG
    CGTGGTCTCA AGCCCTGGAC CCAGTATGCC ATCTTTGTCA AGACCCTGGT CACCTTTTCT
    GATGAACGCC GTACATACGG GGCCAAGAGT GACATCATCT ATGTCCAGAC AGACGCCACT
    AATCCTTCCG TGCCCCTGGA TCCCATCTCA GTTTCTAATT CCTCGTCCCA GATTATTCTG
    AAGTGGAAGC CTCCCTCCGA CCCCAATGGC AATATCACAC ACTACTTGGT GTTCTGGGAG
    AGACAGGCGG AAGACAGTGA GCTGTATGAA CTGGATTATT GCCTCAAAGG GTTGAAGCTG
    CCCTCCCGGA CCTGGTCTCC ACCATTCGAG TCGGAGGGCT CCCAGAAGCA CAACCAGAGT
    GAGTATGAGG AGTCTGCTGG CGAATGCTGC TCCTGCCCAA AGACCGACTC GCAGATCCTC
    AAGGAGCTGG AGGAGTCCTC GTTCAGGAAA ACGTTTGAAG ATTACCTGCA CAACGTGGTT
    TTCATCCCCA GGCCTTCGAG GAAACGCAGA GCCCTTGGTG ATGTGGGGAA TGTGACAGCA
    GCCGTGCCCA CTGCTCTGGG GCTCCCCAAT ACCTCCTCCA CCAGCACGCC CATGAGCTCG
    GAGGAGCACA GACCCTTCGA GAAAGTGGTG AACAAGGAGT CGCTGGTCAT CTCCGGCCTG
    CGTCACTTTA CTGGCTATCG CATCGAGCTT CAGGCTTGCA ACCAAGATTC CCCCGAGGAG
    AGGTGTAGCG TGGCTGCCTA TGTCAGCGCC AGGACCATGC CTGAAGCCAA GGCAGATGAT
    ATTGTCGGCC CCGTGACCCA TGAAATCTTC GAGAACAACG TTGTTCACTT GATGTGGCAG
    GAGCCCAAGG AACCCAACGG TCTGATTGTG CTGTATGAAG TGAGTTACCG ACGATATGGC
    GAGGAGGAGC TGCACCTCTG CGTCTCCCGC CGACATTATG CTCTGGAGCG TGGCTGCCGG
    CTGCGTGGGC TGCTGCCTGG GAATTACAGT GTGCGAGTTC GGGCCACCTC CCTGGCAGGC
    AATGGGTCTT GGACGGAAGC CACCTATTTC TACGTGACAG ACTATTACGT GCCATCAAAT
    ATTGCAAAAA TCATCATCGG TCCCCTCATC TTTGTCTTTC TCTTCAGTGT TGTGATTGGA
    AGTATTTGTC TATTCCTGAG AAAGAGGCAG CCAGATGGGC CATTGGGACC ACTGTATGCT
    TCTTCAAACC CAGAGTACCT CAGTGCCAGC GATGTGTTTC CATGCTCTGT GTATGTGCCG
    GACGAGTGGG AGGTGCCTCG AGAGAAGATC ACACTCCTGC GAGAATTGGG GCAAGGCTCC
    TTTGGCATGG TGTACGAGGG CAACGCCAGG GACATTGTCA AGGGTGAGGC GGAGACGCGC
    GTGGCAGTGA AGACGGTCAA CGAGTCAGCC AGCCTGCGAG AGCGGATCGA GTTTCTCAAC
    GAGGCTTCGG TCATGAAGGG CTTCACCTGC CACCATGTGG TCCGCCTCCT GGGGGTGGTG
    TCCAAAGGCC AGCCAACGCT GGTGGTGATG GAGTTGATGG CTCACGGAGA CCTGAAGAGT
    TACCTCCGTT CCCTGCGGCC TGAGGCTGAG AATAACCCCG GCCGCCCTCC GCCTACCCTG
    CAAGAAATGA TTCAGATGGC GGCAGAGATC GCCGATGGGA TGGCATACCT GAACGCTAAG
    AAGTTCGTGC ACCGGGATCT TGCAGCCCGG AACTGCATGG TTGCCCACGA CTTTACAGTC
    AAAATTGGAG ACTTTGGAAT GACAAGAGAC ATCTATGAAA CGGATTACTA CCGGAAAGGG
    GGCAAAGGTC TGCTCCCCGT GCGGTGGATG GCACCTGAAT CCCTGAAGGA CGGAGTCTTC
    ACCACCTCTT CAGACATGTG GTCTTTTGGT GTGGTCCTTT GGGAAATTAC CAGCTTGGCA
    GAACAGCCTT ACCAAGGTCT ATCAAATGAG CAGGTGCTGA AATTTGTCAT GGATGGAGGG
    TACCTGGATC AACCTGACAA TTGTCCAGAG AGAGTCACCG ACCTGATGCG GATGTGCTGG
    CAGTTCAACC CCAAGATGCG TCCAACCTTC CTGGAGATCG TGGACCTGCT CAAGGACGAC
    CTGCACCCCA GCTTTCCCGA AGTGTCCTTC TTCCACAGCG AGGAGAACAA GGCGCCCGAG
    AGCGAGGAGC TGGAAATGGA GTTTGAGGAC ATGGAGAGCG TGCCCCTGGA CCGGGCCTCG
    CACGCGCAGA GAGAGGAGGC CGGGGGCCGG GATGGAGGGT CGGCGCTGGG GCTAAAGCGA
    AACTATGACG AGCACATCCC CTACACCCAC ATGAACGGGG GCAAGAAAAA CGGACGGATT
    CTGACTTTGC CCCGGTCAAA TCCTTCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.