CACNA1C cDNA ORF clone, Bos taurus(cattle)

The following CACNA1C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa89989 XM_015471361.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89989 XM_015463561.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89990 XM_015463563.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89990 XM_015471362.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89991 XM_015471363.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89991 XM_015463565.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89992 XM_015471364.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89992 XM_015463566.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89993 XM_015471365.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89993 XM_015463567.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89994 XM_015471366.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89994 XM_015463568.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89995 XM_015471367.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89995 XM_015463569.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89996 XM_015463570.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89996 XM_015471368.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89997 XM_015463573.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89997 XM_015471369.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89998 XM_015463574.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89998 XM_015471370.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89999 XM_015463575.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa89999 XM_015471371.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90000 XM_015463577.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90001 XM_015471372.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa90001 XM_015463578.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
OBa45786 XM_010805693.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45786 XM_015463559.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45787 XM_005207303.3
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45787 XM_015463558.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45788 XM_015463560.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45788 XM_010805694.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45789 XM_015463562.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45789 XM_005207302.3
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45790 XM_010805695.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45790 XM_015463564.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45791 XM_010805696.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45791 XM_015463571.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45792 XM_010805697.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45792 XM_015463572.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45793 XM_015463576.1
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa45793 XM_010805698.2
Latest version!
Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
OBa234933 XM_024992746.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234934 XM_024992747.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234935 XM_024992748.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234936 XM_024992749.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234937 XM_024992750.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234938 XM_024992752.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234939 XM_024992753.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234940 XM_024992754.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234941 XM_024992755.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234942 XM_024992756.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234943 XM_024992757.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234944 XM_024992758.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234945 XM_024992759.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234946 XM_024992760.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234947 XM_024992761.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234948 XM_024992763.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00
OBa234949 XM_024992764.1
Latest version!
Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1091.30
$1559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa89989
    Clone ID Related Accession (Same CDS sequence) XM_015471361.1 , XM_015463561.1
    Accession Version XM_015471361.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326847.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_015471361.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471361.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89989
    Clone ID Related Accession (Same CDS sequence) XM_015471361.1 , XM_015463561.1
    Accession Version XM_015463561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319047.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_015463561.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89990
    Clone ID Related Accession (Same CDS sequence) XM_015471362.1 , XM_015463563.1
    Accession Version XM_015471362.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326848.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_015471362.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471362.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89990
    Clone ID Related Accession (Same CDS sequence) XM_015471362.1 , XM_015463563.1
    Accession Version XM_015463563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319049.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_015463563.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89991
    Clone ID Related Accession (Same CDS sequence) XM_015463565.1 , XM_015471363.1
    Accession Version XM_015463565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319051.1
    CDS1919..7129
    Translation

    Target ORF information:

    RefSeq Version XM_015463565.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89991
    Clone ID Related Accession (Same CDS sequence) XM_015463565.1 , XM_015471363.1
    Accession Version XM_015471363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326849.1
    CDS1919..7129
    Translation

    Target ORF information:

    RefSeq Version XM_015471363.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89992
    Clone ID Related Accession (Same CDS sequence) XM_015471364.1 , XM_015463566.1
    Accession Version XM_015471364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326850.1
    CDS1917..7121
    Translation

    Target ORF information:

    RefSeq Version XM_015471364.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89992
    Clone ID Related Accession (Same CDS sequence) XM_015471364.1 , XM_015463566.1
    Accession Version XM_015463566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319052.1
    CDS1917..7121
    Translation

    Target ORF information:

    RefSeq Version XM_015463566.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89993
    Clone ID Related Accession (Same CDS sequence) XM_015471365.1 , XM_015463567.1
    Accession Version XM_015471365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326851.1
    CDS1918..7122
    Translation

    Target ORF information:

    RefSeq Version XM_015471365.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89993
    Clone ID Related Accession (Same CDS sequence) XM_015471365.1 , XM_015463567.1
    Accession Version XM_015463567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5205bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319053.1
    CDS1918..7122
    Translation

    Target ORF information:

    RefSeq Version XM_015463567.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG
    AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC
    CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC
    CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89994
    Clone ID Related Accession (Same CDS sequence) XM_015471366.1 , XM_015463568.1
    Accession Version XM_015471366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC
    ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326852.1
    CDS1918..7119
    Translation

    Target ORF information:

    RefSeq Version XM_015471366.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC
    ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89994
    Clone ID Related Accession (Same CDS sequence) XM_015471366.1 , XM_015463568.1
    Accession Version XM_015463568.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC
    ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319054.1
    CDS1918..7119
    Translation

    Target ORF information:

    RefSeq Version XM_015463568.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC
    ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89995
    Clone ID Related Accession (Same CDS sequence) XM_015471367.1 , XM_015463569.1
    Accession Version XM_015471367.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC
    GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326853.1
    CDS1918..7119
    Translation

    Target ORF information:

    RefSeq Version XM_015471367.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471367.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC
    GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89995
    Clone ID Related Accession (Same CDS sequence) XM_015471367.1 , XM_015463569.1
    Accession Version XM_015463569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5202bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC
    GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319055.1
    CDS1918..7119
    Translation

    Target ORF information:

    RefSeq Version XM_015463569.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG
    CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA
    GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG
    ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT
    GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC
    TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG
    GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG
    AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG
    GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC
    AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC
    CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG
    AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT
    TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA
    GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG
    ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC
    ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG
    GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG
    AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG
    CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG
    GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG
    TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT
    GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC
    ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC
    AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG
    AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG
    GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG
    TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG
    AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC
    GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG
    CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT
    CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC
    ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT
    TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC
    TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG
    CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG
    AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG
    CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC
    GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG
    CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA
    ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC
    AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC
    GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC
    ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC
    TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG
    CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC
    AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC
    GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC
    TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC
    AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG
    CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC
    GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT
    ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG
    CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC
    AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC
    GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC
    AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT
    GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG
    TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC
    TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC
    ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG
    GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC
    CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC
    CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG
    ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG
    GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA
    CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG
    GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC
    AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG
    GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC
    GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG
    CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89996
    Clone ID Related Accession (Same CDS sequence) XM_015463570.1 , XM_015471368.1
    Accession Version XM_015463570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC
    CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG
    TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC
    TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319056.1
    CDS1918..7116
    Translation

    Target ORF information:

    RefSeq Version XM_015463570.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC
    CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG
    TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC
    TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89996
    Clone ID Related Accession (Same CDS sequence) XM_015463570.1 , XM_015471368.1
    Accession Version XM_015471368.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5199bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC
    CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG
    TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC
    TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326854.1
    CDS1918..7116
    Translation

    Target ORF information:

    RefSeq Version XM_015471368.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471368.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC
    CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG
    TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC
    TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89997
    Clone ID Related Accession (Same CDS sequence) XM_015463573.1 , XM_015471369.1
    Accession Version XM_015463573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319059.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_015463573.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89997
    Clone ID Related Accession (Same CDS sequence) XM_015463573.1 , XM_015471369.1
    Accession Version XM_015471369.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326855.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_015471369.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471369.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89998
    Clone ID Related Accession (Same CDS sequence) XM_015471370.1 , XM_015463574.1
    Accession Version XM_015471370.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326856.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_015471370.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471370.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89998
    Clone ID Related Accession (Same CDS sequence) XM_015471370.1 , XM_015463574.1
    Accession Version XM_015463574.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319060.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_015463574.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463574.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89999
    Clone ID Related Accession (Same CDS sequence) XM_015463575.1 , XM_015471371.1
    Accession Version XM_015463575.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319061.1
    CDS1919..7096
    Translation

    Target ORF information:

    RefSeq Version XM_015463575.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463575.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa89999
    Clone ID Related Accession (Same CDS sequence) XM_015463575.1 , XM_015471371.1
    Accession Version XM_015471371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326857.1
    CDS1919..7096
    Translation

    Target ORF information:

    RefSeq Version XM_015471371.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90000
    Clone ID Related Accession (Same CDS sequence) XM_015463577.1
    Accession Version XM_015463577.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACGCCA ACGCGGCCGC GGGGCTGGCA CCTGAGCACA TCCCCACACC GGGGGCCGCC 
    CTGTCCTGGC AGGCCGCCAT CGACGCGGCC CGGCAGGCCA AGCTGATGGG CAGTGCTGGC
    AACGCAACCA TCTCCACGGT CAGCTCCACG CAGCGGAAAC GGCAGCAGTA TGGGAAGCCC
    AAGAAGCAGG GCAGCACGAC AGCCACCCGC CCGCCCCGCG CCCTGCTCTG CCTGACCCTA
    AAGAACCCCA TCCGGAGGGC CTGCATCAGC ATCGTCGAGT GGAAGCCATT TGAAATCATT
    ATTTTACTGA CTATTTTTGC CAATTGTGTG GCCTTAGCGA TCTATATTCC CTTCCCAGAG
    GACGACTCCA ACGCCACGAA TTCCAACCTG GAGCGGGTGG AGTATCTCTT CCTCATCATT
    TTTACTGTGG AAGCATTTTT AAAAGTCATA GCCTACGGAC TCCTCTTTCA CCCCAACGCT
    TACCTCCGCA ATGGCTGGAA CTTACTAGAC TTTATCATCG TGGTTGTCGG GCTTTTCAGC
    GCGATCTTAG AGCAAGCGAC CAAAGCCGAT GGGGCCAACG CCCTGGGAGG GAAGGGTGCT
    GGATTCGACG TAAAGGCGCT GAGGGCGTTC CGTGTGCTAC GGCCCCTGCG GTTGGTGTCC
    GGGGTCCCGA GTCTCCAGGT GGTCTTGAAT TCCATCATCA AGGCCATGGT GCCCCTGCTG
    CACATCGCCC TGCTCGTGCT GTTCGTCATC ATCATCTATG CCATCATCGG CCTGGAGCTC
    TTCATGGGCA AGATGCATAA GACCTGCTAC AACCAGGAGG GCATTGCAGA TGTCCCAGCA
    GAAGACGACC CCTCGCCCTG TGCCCTGGAG ACAGGCCACG GGCGGCAGTG CCAGAATGGC
    ACGGTGTGCA AACCAGGCTG GGATGGACCC AAGCACGGCA TCACCAATTT CGACAACTTC
    GCCTTCGCCA TGCTGACTGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACGTCCTC
    TACTGGGTCA ATGATGCCGT GGGAAGGGAC TGGCCCTGGA TCTATTTTGT CACATTAATC
    ATCATAGGTT CATTCTTTGT TCTTAACTTG GTTCTCGGTG TCCTTAGCGG AGAGTTCTCC
    AAAGAGCGAG AGAAGGCCAA GGCCCGGGGA GACTTCCAGA AGCTGCGAGA GAAGCAGCAG
    CTGGAGGAGG ACCTGAAGGG CTACCTGGAC TGGATCACGC AGGCCGAGGA CATCGACCCC
    GAGAATGAGG ACGAGGGCCT GGACGAGGAG AAACCCCGAA ACATGAGCAT GCCCACCAGT
    GAGACCGAGT CTGTCAACAC CGAAAACGTG GCTGGAGGGG ACATCGAGGG AGAGAGCTGC
    GGGGCCAGGC TGGCCCACCG GATCTCCAAG TCAAAGTTCA GCCGCTACTG GCGCCGGTGG
    AATCGGTTCT GCAGGAGGAA GTGCCGCGCG GCCGTGAAGT CCAGCATCTT CTACTGGCTG
    GTCATCTTCC TGGTTTTCCT CAACACACTC ACCATCGCCT CCGAGCACTA CAACCAGCCC
    CACTGGCTCA CGGAAGTCCA AGACACGGCC AACAAGGCGC TGCTGGCCCT GTTCACGGCG
    GAGATGCTGC TCAAGATGTA CAGCCTGGGC CTGCAGGCCT ACTTCGTGTC GCTCTTCAAC
    CGCTTCGACT GCTTCATCGT GTGCGGGGGC ATCCTGGAGA CCATCCTGGT GGAGACCAAG
    ATCATGTCGC CGTTGGGCAT CTCCGTGCTG CGTTGCGTGC GCCTCCTTCG GATATTTAAG
    ATCACAAGGT ACTGGAACTC CCTGAGCAAC CTGGTGGCAT CCCTGCTGAA CTCAGTGCGC
    TCCATTGCCT CGCTGCTGCT GCTGCTCTTC CTCTTCATCA TCATCTTCTC CCTCCTGGGG
    ATGCAGCTCT TTGGGGGGAA GTTCAACTTT GATGAGATGC AGACCCGGAG GAGCACCTTT
    GACAACTTCC CGCAGTCCCT CCTCACCGTG TTTCAGATCC TGACCGGGGA GGACTGGAAT
    TCGGTGATGT ATGATGGGAT CATGGCTTAT GGCGGCCCCT CTTTTCCAGG GATGTTAGTC
    TGTATTTACT TCATCATCCT CTTCATCTGT GGAAATTATA TCCTACTGAA CGTGTTCTTG
    GCCATTGCTG TGGACAACCT GGCTGACGCG GAGAGCCTCA CATCTGCCCA GAAGGAGGAG
    GAAGAGGAGA AGGAGAGGAA GAAGCTAGCC AGGACCGCCA GCCCAGAGAA GAAGCAAGAG
    GTGGTAGAGA AGCCGGCCGT GGAGGAGACC AAGGAGGAGA AAATCGAGCT GAAATCCATC
    ACGGCAGATG GAGAGTCCCC ACCCGCCACC AAGATCAACA TGGATGACCT CCAGCCCAAC
    GAGAATGAGG ACAAGAGCTC CTACCCCAAC CAGGAAACCA CAGGAGAAGA GGACGAGGAG
    GAGCCCGAGA TGCCCGTGGG CCCCCGCCCG CGCCCCCTCT CTGAGCTGCA CCTGAAGGAG
    AAGGCAGTGC CCATGCCGGA AGCCAGCGCC TTCTTCATCT TCAGCCCCAG CAACAGGTTC
    CGCCTCCAGT GCCATCGCAT CGTCAATGAC TCCATCTTCA CCAACCTGAT CCTCTTCTTC
    ATCCTGCTCA GCAGCATCTC CCTGGCCGCC GAGGACCCCG TGCAGCACAC CTCCTTCAGG
    AACCACATTC TGTTTTATTT TGACATTGTT TTCACTACCA TTTTCACCAT TGAGATTGCC
    CTGAAGATGA CGGCCTACGG GGCCTTCCTG CACAAGGGCT CCTTCTGCCG CAACTACTTC
    AACATCCTGG ACCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTCGGCAT CCAGTCCAGT
    GCCATCAACG TGGTGAAGAT CCTGCGAGTC CTGAGGGTGC TCCGGCCACT GAGGGCCATC
    AACAGGGCCA AGGGGCTGAA GCACGTCGTG CAGTGCGTGT TCGTGGCCAT CCGCACCATC
    GGGAACATTG TGATCGTCAC CACGCTGCTC CAGTTCATGT TCGCCTGCAT TGGGGTCCAG
    CTCTTCAAGG GAAAGCTCTA CACCTGTTCG GACAGTTCCA AACAGACAGA GGCTGAATGC
    AAGGGGAACT ACATAACATA CAAGGATGGG GAGGTGGACC ACCCCATCAT CCAGCCCCGC
    AGCTGGGAGA ACAGCAAGTT TGACTTTGAC AATGTCCTGG CAGCCATGAT GGCCCTGTTC
    ACCGTCTCCA CCTTCGAGGG ATGGCCAGAG TTGCTGTACC GCTCCATCGA CTCTCACACG
    GAGGACAAGG GTCCCATATA CAACTACCGC GTGGAGATCT CCGTCTTCTT CATCATCTAC
    ATCATCATCA TTGCCTTCTT CATGATGAAC ATCTTCGTGG GTTTCGTCAT CGTCACCTTC
    CAGGAGCAGG GGGAGCAGGA ATACAAGAAC TGCGAGCTGG ACAAGAACCA GCGGCAGTGT
    GTGGAGTATG CCCTCAAGGC CCGGCCCCTG CGGAGGTACA TCCCGAAAAA CCAGCACCAG
    TACAAGGTGT GGTACGTGGT CAACTCCACC TACTTCGAGT ACCTGATGTT CGTTCTCATT
    CTGCTCAACA CCATCTGCCT GGCCATGCAG CACTACGGTC AGAGCTGTCT GTTCAAAATC
    GCCATGAACA TCCTCAACAT GCTCTTCACC GGCCTCTTCA CAGTGGAGAT GGTCCTGAAG
    CTCATCGCCT TCAAGCCCAA GCACTATTTC TGTGATGCAT GGAACACATT TGACGCCTTG
    ATTGTTGTGG GTAGCATTGT TGATATAGCC ATCACCGAGG TAAACCCAGC TGAACATACC
    CAATGCTCTC CCTTTATGAA CGCGGAAGAG AACTCTCGCA TCTCCATCAC CTTCTTCCGC
    CTCTTCCGGG TCATGCGTCT GGTCAAGCTG CTGAGCCGCG GGGAGGGCAT CCGGACACTG
    CTGTGGACCT TCATCAAGTC CTTCCAGGCC CTACCCTATG TGGCCCTCCT GATCGTGATG
    CTGTTTTTCA TCTACGCCGT GATCGGGATG CAGGTGTTTG GCAAAATTGC CCTGAATGAC
    ACGACAGAGA TCAACAGGAA CAACAACTTC CAGACCTTCC CCCAGGCCGT GCTGCTCCTC
    TTCAGGTGCG CCACTGGGGA GGCCTGGCAG GACATCATGC TGGCCTGCAT GCCTGGCAAG
    AAGTGCGCCC CCGAGTCAGA GCCGGGCAAC AGCACAGAGG GGGAGACGCC CTGTGGCAGC
    AGCTTCGCCG TCTTCTACTT CATCAGCTTC TACATGCTCT GCGCCTTCCT GATCATCAAC
    CTCTTCGTAG CCGTCATCAT GGACAACTTT GACTACCTGA CGAGGGACTG GTCCATCCTG
    GGTCCCCACC ACCTGGACGA ATTTAAGAGA ATTTGGGCAG AGTATGACCC TGAAGCCAAG
    GGACGCATCA AACACCTGGA TGTGGTGACC CTGCTCCGAC GGATTCAGCC CCCCCTGGGT
    TTTGGGAAGC TGTGCCCTCA CCGCGTGGCT TGCAAACGCC TGGTCTCCAT GAACATGCCC
    CTGAACAGCG ACGGGACTGT CATGTTCAAT GCCACGCTGT TTGCCCTGGT CCGTACAGCT
    CTGAGGATCA AGACGGAAGG GAACCTGGAA CAGGCCAATG AGGAGCTGCG GGCCATCATC
    AAGAAGATCT GGAAACGGAC CAGCATGAAG CTGCTGGACC AAGTGGTGCC GCCCGCAGGA
    GATGACGAGG TCACGGTGGG CAAGTTTTAC GCTACTTTCC TGATCCAGGA GTATTTCCGG
    AAGTTCAAGA AGCGCAAAGA GCAAGGCCTG GTGGGCAAGC CCTCCCAGAG GAACGCCCTG
    TCCCTGCAGG TGAGGGGCCC TTGCCCCTGG GGACCTGGCA CCTCCTCCCA CCTCTTCCAG
    GTTCCACGCT CCCACGGGTC CCTTCCGGAA ATTCCGAGCC TGCAGGGGGA CCCTCCCCTC
    CCGCCCTGCC TCCTGCACTC ACTGCCCTCT GTAGTTCTGG ACTCTCAGCC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319063.1
    CDS424..5460
    Translation

    Target ORF information:

    RefSeq Version XM_015463577.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463577.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAACGCCA ACGCGGCCGC GGGGCTGGCA CCTGAGCACA TCCCCACACC GGGGGCCGCC 
    CTGTCCTGGC AGGCCGCCAT CGACGCGGCC CGGCAGGCCA AGCTGATGGG CAGTGCTGGC
    AACGCAACCA TCTCCACGGT CAGCTCCACG CAGCGGAAAC GGCAGCAGTA TGGGAAGCCC
    AAGAAGCAGG GCAGCACGAC AGCCACCCGC CCGCCCCGCG CCCTGCTCTG CCTGACCCTA
    AAGAACCCCA TCCGGAGGGC CTGCATCAGC ATCGTCGAGT GGAAGCCATT TGAAATCATT
    ATTTTACTGA CTATTTTTGC CAATTGTGTG GCCTTAGCGA TCTATATTCC CTTCCCAGAG
    GACGACTCCA ACGCCACGAA TTCCAACCTG GAGCGGGTGG AGTATCTCTT CCTCATCATT
    TTTACTGTGG AAGCATTTTT AAAAGTCATA GCCTACGGAC TCCTCTTTCA CCCCAACGCT
    TACCTCCGCA ATGGCTGGAA CTTACTAGAC TTTATCATCG TGGTTGTCGG GCTTTTCAGC
    GCGATCTTAG AGCAAGCGAC CAAAGCCGAT GGGGCCAACG CCCTGGGAGG GAAGGGTGCT
    GGATTCGACG TAAAGGCGCT GAGGGCGTTC CGTGTGCTAC GGCCCCTGCG GTTGGTGTCC
    GGGGTCCCGA GTCTCCAGGT GGTCTTGAAT TCCATCATCA AGGCCATGGT GCCCCTGCTG
    CACATCGCCC TGCTCGTGCT GTTCGTCATC ATCATCTATG CCATCATCGG CCTGGAGCTC
    TTCATGGGCA AGATGCATAA GACCTGCTAC AACCAGGAGG GCATTGCAGA TGTCCCAGCA
    GAAGACGACC CCTCGCCCTG TGCCCTGGAG ACAGGCCACG GGCGGCAGTG CCAGAATGGC
    ACGGTGTGCA AACCAGGCTG GGATGGACCC AAGCACGGCA TCACCAATTT CGACAACTTC
    GCCTTCGCCA TGCTGACTGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACGTCCTC
    TACTGGGTCA ATGATGCCGT GGGAAGGGAC TGGCCCTGGA TCTATTTTGT CACATTAATC
    ATCATAGGTT CATTCTTTGT TCTTAACTTG GTTCTCGGTG TCCTTAGCGG AGAGTTCTCC
    AAAGAGCGAG AGAAGGCCAA GGCCCGGGGA GACTTCCAGA AGCTGCGAGA GAAGCAGCAG
    CTGGAGGAGG ACCTGAAGGG CTACCTGGAC TGGATCACGC AGGCCGAGGA CATCGACCCC
    GAGAATGAGG ACGAGGGCCT GGACGAGGAG AAACCCCGAA ACATGAGCAT GCCCACCAGT
    GAGACCGAGT CTGTCAACAC CGAAAACGTG GCTGGAGGGG ACATCGAGGG AGAGAGCTGC
    GGGGCCAGGC TGGCCCACCG GATCTCCAAG TCAAAGTTCA GCCGCTACTG GCGCCGGTGG
    AATCGGTTCT GCAGGAGGAA GTGCCGCGCG GCCGTGAAGT CCAGCATCTT CTACTGGCTG
    GTCATCTTCC TGGTTTTCCT CAACACACTC ACCATCGCCT CCGAGCACTA CAACCAGCCC
    CACTGGCTCA CGGAAGTCCA AGACACGGCC AACAAGGCGC TGCTGGCCCT GTTCACGGCG
    GAGATGCTGC TCAAGATGTA CAGCCTGGGC CTGCAGGCCT ACTTCGTGTC GCTCTTCAAC
    CGCTTCGACT GCTTCATCGT GTGCGGGGGC ATCCTGGAGA CCATCCTGGT GGAGACCAAG
    ATCATGTCGC CGTTGGGCAT CTCCGTGCTG CGTTGCGTGC GCCTCCTTCG GATATTTAAG
    ATCACAAGGT ACTGGAACTC CCTGAGCAAC CTGGTGGCAT CCCTGCTGAA CTCAGTGCGC
    TCCATTGCCT CGCTGCTGCT GCTGCTCTTC CTCTTCATCA TCATCTTCTC CCTCCTGGGG
    ATGCAGCTCT TTGGGGGGAA GTTCAACTTT GATGAGATGC AGACCCGGAG GAGCACCTTT
    GACAACTTCC CGCAGTCCCT CCTCACCGTG TTTCAGATCC TGACCGGGGA GGACTGGAAT
    TCGGTGATGT ATGATGGGAT CATGGCTTAT GGCGGCCCCT CTTTTCCAGG GATGTTAGTC
    TGTATTTACT TCATCATCCT CTTCATCTGT GGAAATTATA TCCTACTGAA CGTGTTCTTG
    GCCATTGCTG TGGACAACCT GGCTGACGCG GAGAGCCTCA CATCTGCCCA GAAGGAGGAG
    GAAGAGGAGA AGGAGAGGAA GAAGCTAGCC AGGACCGCCA GCCCAGAGAA GAAGCAAGAG
    GTGGTAGAGA AGCCGGCCGT GGAGGAGACC AAGGAGGAGA AAATCGAGCT GAAATCCATC
    ACGGCAGATG GAGAGTCCCC ACCCGCCACC AAGATCAACA TGGATGACCT CCAGCCCAAC
    GAGAATGAGG ACAAGAGCTC CTACCCCAAC CAGGAAACCA CAGGAGAAGA GGACGAGGAG
    GAGCCCGAGA TGCCCGTGGG CCCCCGCCCG CGCCCCCTCT CTGAGCTGCA CCTGAAGGAG
    AAGGCAGTGC CCATGCCGGA AGCCAGCGCC TTCTTCATCT TCAGCCCCAG CAACAGGTTC
    CGCCTCCAGT GCCATCGCAT CGTCAATGAC TCCATCTTCA CCAACCTGAT CCTCTTCTTC
    ATCCTGCTCA GCAGCATCTC CCTGGCCGCC GAGGACCCCG TGCAGCACAC CTCCTTCAGG
    AACCACATTC TGTTTTATTT TGACATTGTT TTCACTACCA TTTTCACCAT TGAGATTGCC
    CTGAAGATGA CGGCCTACGG GGCCTTCCTG CACAAGGGCT CCTTCTGCCG CAACTACTTC
    AACATCCTGG ACCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTCGGCAT CCAGTCCAGT
    GCCATCAACG TGGTGAAGAT CCTGCGAGTC CTGAGGGTGC TCCGGCCACT GAGGGCCATC
    AACAGGGCCA AGGGGCTGAA GCACGTCGTG CAGTGCGTGT TCGTGGCCAT CCGCACCATC
    GGGAACATTG TGATCGTCAC CACGCTGCTC CAGTTCATGT TCGCCTGCAT TGGGGTCCAG
    CTCTTCAAGG GAAAGCTCTA CACCTGTTCG GACAGTTCCA AACAGACAGA GGCTGAATGC
    AAGGGGAACT ACATAACATA CAAGGATGGG GAGGTGGACC ACCCCATCAT CCAGCCCCGC
    AGCTGGGAGA ACAGCAAGTT TGACTTTGAC AATGTCCTGG CAGCCATGAT GGCCCTGTTC
    ACCGTCTCCA CCTTCGAGGG ATGGCCAGAG TTGCTGTACC GCTCCATCGA CTCTCACACG
    GAGGACAAGG GTCCCATATA CAACTACCGC GTGGAGATCT CCGTCTTCTT CATCATCTAC
    ATCATCATCA TTGCCTTCTT CATGATGAAC ATCTTCGTGG GTTTCGTCAT CGTCACCTTC
    CAGGAGCAGG GGGAGCAGGA ATACAAGAAC TGCGAGCTGG ACAAGAACCA GCGGCAGTGT
    GTGGAGTATG CCCTCAAGGC CCGGCCCCTG CGGAGGTACA TCCCGAAAAA CCAGCACCAG
    TACAAGGTGT GGTACGTGGT CAACTCCACC TACTTCGAGT ACCTGATGTT CGTTCTCATT
    CTGCTCAACA CCATCTGCCT GGCCATGCAG CACTACGGTC AGAGCTGTCT GTTCAAAATC
    GCCATGAACA TCCTCAACAT GCTCTTCACC GGCCTCTTCA CAGTGGAGAT GGTCCTGAAG
    CTCATCGCCT TCAAGCCCAA GCACTATTTC TGTGATGCAT GGAACACATT TGACGCCTTG
    ATTGTTGTGG GTAGCATTGT TGATATAGCC ATCACCGAGG TAAACCCAGC TGAACATACC
    CAATGCTCTC CCTTTATGAA CGCGGAAGAG AACTCTCGCA TCTCCATCAC CTTCTTCCGC
    CTCTTCCGGG TCATGCGTCT GGTCAAGCTG CTGAGCCGCG GGGAGGGCAT CCGGACACTG
    CTGTGGACCT TCATCAAGTC CTTCCAGGCC CTACCCTATG TGGCCCTCCT GATCGTGATG
    CTGTTTTTCA TCTACGCCGT GATCGGGATG CAGGTGTTTG GCAAAATTGC CCTGAATGAC
    ACGACAGAGA TCAACAGGAA CAACAACTTC CAGACCTTCC CCCAGGCCGT GCTGCTCCTC
    TTCAGGTGCG CCACTGGGGA GGCCTGGCAG GACATCATGC TGGCCTGCAT GCCTGGCAAG
    AAGTGCGCCC CCGAGTCAGA GCCGGGCAAC AGCACAGAGG GGGAGACGCC CTGTGGCAGC
    AGCTTCGCCG TCTTCTACTT CATCAGCTTC TACATGCTCT GCGCCTTCCT GATCATCAAC
    CTCTTCGTAG CCGTCATCAT GGACAACTTT GACTACCTGA CGAGGGACTG GTCCATCCTG
    GGTCCCCACC ACCTGGACGA ATTTAAGAGA ATTTGGGCAG AGTATGACCC TGAAGCCAAG
    GGACGCATCA AACACCTGGA TGTGGTGACC CTGCTCCGAC GGATTCAGCC CCCCCTGGGT
    TTTGGGAAGC TGTGCCCTCA CCGCGTGGCT TGCAAACGCC TGGTCTCCAT GAACATGCCC
    CTGAACAGCG ACGGGACTGT CATGTTCAAT GCCACGCTGT TTGCCCTGGT CCGTACAGCT
    CTGAGGATCA AGACGGAAGG GAACCTGGAA CAGGCCAATG AGGAGCTGCG GGCCATCATC
    AAGAAGATCT GGAAACGGAC CAGCATGAAG CTGCTGGACC AAGTGGTGCC GCCCGCAGGA
    GATGACGAGG TCACGGTGGG CAAGTTTTAC GCTACTTTCC TGATCCAGGA GTATTTCCGG
    AAGTTCAAGA AGCGCAAAGA GCAAGGCCTG GTGGGCAAGC CCTCCCAGAG GAACGCCCTG
    TCCCTGCAGG TGAGGGGCCC TTGCCCCTGG GGACCTGGCA CCTCCTCCCA CCTCTTCCAG
    GTTCCACGCT CCCACGGGTC CCTTCCGGAA ATTCCGAGCC TGCAGGGGGA CCCTCCCCTC
    CCGCCCTGCC TCCTGCACTC ACTGCCCTCT GTAGTTCTGG ACTCTCAGCC CCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90001
    Clone ID Related Accession (Same CDS sequence) XM_015463578.1 , XM_015471372.1
    Accession Version XM_015463578.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319064.1
    CDS1919..7096
    Translation

    Target ORF information:

    RefSeq Version XM_015463578.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463578.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa90001
    Clone ID Related Accession (Same CDS sequence) XM_015463578.1 , XM_015471372.1
    Accession Version XM_015471372.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015326858.1
    CDS1919..7096
    Translation

    Target ORF information:

    RefSeq Version XM_015471372.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015471372.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45786
    Clone ID Related Accession (Same CDS sequence) XM_010805693.2 , XM_015463559.1
    Accession Version XM_010805693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803995.1
    CDS1917..7127
    Translation

    Target ORF information:

    RefSeq Version XM_010805693.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45786
    Clone ID Related Accession (Same CDS sequence) XM_010805693.2 , XM_015463559.1
    Accession Version XM_015463559.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319045.1
    CDS1917..7127
    Translation

    Target ORF information:

    RefSeq Version XM_015463559.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463559.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45787
    Clone ID Related Accession (Same CDS sequence) XM_005207303.3 , XM_015463558.1
    Accession Version XM_005207303.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207303.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207360.2
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_005207303.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207303.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45787
    Clone ID Related Accession (Same CDS sequence) XM_005207303.3 , XM_015463558.1
    Accession Version XM_015463558.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319044.1
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_015463558.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463558.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45788
    Clone ID Related Accession (Same CDS sequence) XM_010805694.2 , XM_015463560.1
    Accession Version XM_010805694.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803996.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_010805694.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805694.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45788
    Clone ID Related Accession (Same CDS sequence) XM_010805694.2 , XM_015463560.1
    Accession Version XM_015463560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319046.1
    CDS1918..7128
    Translation

    Target ORF information:

    RefSeq Version XM_015463560.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45789
    Clone ID Related Accession (Same CDS sequence) XM_005207302.3 , XM_015463562.1
    Accession Version XM_005207302.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207359.2
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_005207302.3
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207302.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45789
    Clone ID Related Accession (Same CDS sequence) XM_005207302.3 , XM_015463562.1
    Accession Version XM_015463562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319048.1
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_015463562.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45790
    Clone ID Related Accession (Same CDS sequence) XM_010805695.2 , XM_015463564.1
    Accession Version XM_010805695.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803997.1
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_010805695.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805695.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45790
    Clone ID Related Accession (Same CDS sequence) XM_010805695.2 , XM_015463564.1
    Accession Version XM_015463564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5211bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319050.1
    CDS1..5211
    Translation

    Target ORF information:

    RefSeq Version XM_015463564.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA
    CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC
    TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45791
    Clone ID Related Accession (Same CDS sequence) XM_010805696.2 , XM_015463571.1
    Accession Version XM_010805696.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803998.1
    CDS1917..7094
    Translation

    Target ORF information:

    RefSeq Version XM_010805696.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805696.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45791
    Clone ID Related Accession (Same CDS sequence) XM_010805696.2 , XM_015463571.1
    Accession Version XM_015463571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319057.1
    CDS1917..7094
    Translation

    Target ORF information:

    RefSeq Version XM_015463571.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45792
    Clone ID Related Accession (Same CDS sequence) XM_015463572.1 , XM_010805697.2
    Accession Version XM_015463572.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319058.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_015463572.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45792
    Clone ID Related Accession (Same CDS sequence) XM_015463572.1 , XM_010805697.2
    Accession Version XM_010805697.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010803999.1
    CDS1918..7095
    Translation

    Target ORF information:

    RefSeq Version XM_010805697.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805697.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC
    ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC
    CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG
    GACTCTCAGC CCCTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45793
    Clone ID Related Accession (Same CDS sequence) XM_010805698.2 , XM_015463576.1
    Accession Version XM_010805698.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC
    TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG
    GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT
    CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804000.1
    CDS1917..7088
    Translation

    Target ORF information:

    RefSeq Version XM_010805698.2
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010805698.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC
    TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG
    GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT
    CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45793
    Clone ID Related Accession (Same CDS sequence) XM_010805698.2 , XM_015463576.1
    Accession Version XM_015463576.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5172bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC
    TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG
    GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT
    CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015319062.1
    CDS1917..7088
    Translation

    Target ORF information:

    RefSeq Version XM_015463576.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015463576.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC
    TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG
    GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT
    CAGCCCCTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234933
    Clone ID Related Accession (Same CDS sequence) XM_024992746.1
    Accession Version XM_024992746.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848514.1
    CDS1835..8338
    Translation

    Target ORF information:

    RefSeq Version XM_024992746.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992746.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234934
    Clone ID Related Accession (Same CDS sequence) XM_024992747.1
    Accession Version XM_024992747.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848515.1
    CDS1836..8339
    Translation

    Target ORF information:

    RefSeq Version XM_024992747.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992747.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234935
    Clone ID Related Accession (Same CDS sequence) XM_024992748.1
    Accession Version XM_024992748.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848516.1
    CDS1837..8340
    Translation

    Target ORF information:

    RefSeq Version XM_024992748.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992748.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234936
    Clone ID Related Accession (Same CDS sequence) XM_024992749.1
    Accession Version XM_024992749.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848517.1
    CDS1836..8339
    Translation

    Target ORF information:

    RefSeq Version XM_024992749.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992749.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234937
    Clone ID Related Accession (Same CDS sequence) XM_024992750.1
    Accession Version XM_024992750.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848518.1
    CDS1838..8341
    Translation

    Target ORF information:

    RefSeq Version XM_024992750.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992750.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234938
    Clone ID Related Accession (Same CDS sequence) XM_024992752.1
    Accession Version XM_024992752.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848520.1
    CDS1838..8341
    Translation

    Target ORF information:

    RefSeq Version XM_024992752.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992752.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234939
    Clone ID Related Accession (Same CDS sequence) XM_024992753.1
    Accession Version XM_024992753.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848521.1
    CDS1839..8342
    Translation

    Target ORF information:

    RefSeq Version XM_024992753.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992753.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC
    TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC
    CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG
    ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT
    TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA
    GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG
    TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC
    GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC
    GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC
    GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC
    CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC
    ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG
    AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC
    AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG
    ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG
    ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC
    GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC
    AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG
    CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG
    GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA
    GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG
    AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC
    AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC
    ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC
    AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG
    GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC
    AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC
    TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC
    AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC
    CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC
    TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG
    GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA
    GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA
    CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG
    CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC
    GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG
    CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT
    GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC
    CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG
    GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC
    ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG
    GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC
    AGGGCCTTCG CCAGCGGCCT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234940
    Clone ID Related Accession (Same CDS sequence) XM_024992754.1
    Accession Version XM_024992754.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT
    GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG
    ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT
    GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC
    GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA
    GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC
    ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC
    GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC
    CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC
    CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG
    GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG
    GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG
    GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA
    GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC
    GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG
    GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC
    ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG
    GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC
    CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG
    TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC
    AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG
    TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG
    GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG
    CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA
    GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC
    TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848522.1
    CDS1835..8332
    Translation

    Target ORF information:

    RefSeq Version XM_024992754.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992754.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT
    GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG
    ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT
    GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC
    GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA
    GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC
    ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC
    GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC
    CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC
    CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG
    GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG
    GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG
    GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA
    GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC
    GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG
    GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC
    ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG
    GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC
    CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG
    TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC
    AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG
    TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG
    GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG
    CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA
    GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC
    TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234941
    Clone ID Related Accession (Same CDS sequence) XM_024992755.1
    Accession Version XM_024992755.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT
    GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG
    ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT
    GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC
    GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA
    GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC
    ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC
    GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC
    CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC
    CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG
    GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG
    GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG
    GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA
    GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC
    GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG
    GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC
    ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG
    GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC
    CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG
    TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC
    AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG
    TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG
    GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG
    CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA
    GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC
    TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848523.1
    CDS1838..8335
    Translation

    Target ORF information:

    RefSeq Version XM_024992755.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992755.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC
    TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC
    ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC
    ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC
    TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC
    AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC
    ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC
    GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC
    TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC
    GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC
    CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA
    CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG
    TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC
    GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG
    ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG
    AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC
    ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG
    CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT
    GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG
    ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT
    GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC
    GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA
    GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC
    ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC
    GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC
    CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC
    CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG
    GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG
    GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG
    GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA
    GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC
    GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG
    GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC
    ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG
    GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC
    CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG
    TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC
    AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG
    TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG
    GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG
    CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA
    GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC
    TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234942
    Clone ID Related Accession (Same CDS sequence) XM_024992756.1
    Accession Version XM_024992756.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGCTGGCCTG
    CGCACCCTGC ACGATCTGGG GCCCGAGATC CGCCGGGCCA TCTCTGGGGA CCTGACCGCC
    GAGGAGGAGC TGGACAAGGC CATGAAGGAG GCTGTGTCTG CAGCCTCAGA AGATGACATC
    TTCAGGAGGG CCGGCGGCCT GTTCGGCAAC CACGTGGGCT ACTACCCGAG CGACGGCCGG
    GGCGCCTTCC CCCAGACCTT CACCACCCAG CGCCCCCTGC ACATCAGCAA GGCAGGCAAC
    AGCCAGGGGG ACACCGAGTC CCCCTCCCAC GAGAAACTGG TGGACTCCAC CTTCACCCCC
    AGCAGCTACT CATCCACGGG CTCCAACGCC AACATCAACA ACGCCAACAA CACCGCCCTG
    GGCCGCTGCC CCCGCCCCGC GCTCCACTCC GGTGCTGTCA GCACCTTGGA GGCCCACGGG
    CCCCCCTTGT CCCCTGCCAT CCGGGTGCAG GAGGCCGCGT GGAGGCACAG CTCCCAGAGG
    TGCCCCTCCC GGGACAGCCC GATAGCCATG CTGTGTCAGG AAGAGGCCTC TGGGGACGAG
    ACCTGTGCCG TGCACGTGGA CGAGAACGCA GAGCACAGCA GCGAGCCCAG CTTGGTCTCC
    ACGGAGATGC TCTCCTACCA GGATGACGAG AATCGGCAGC TGACTCCCCC CGAGGAGGAC
    AGGAGGGACA TCCGTCTGTC CCCAAAGAGG GGCTTCCTGC GCTCCGCCTC CCTAGGTCGA
    AGGGCCTCCT TCCACCTTGA ATGTCTGAAG CGACAGAAGA ATCCTGGGGG AGACGTCTCT
    CAGAACACAG TCCTGCCTTT GCACCTGGTT CACCATCAGG CGCTGGCAGT GGCGGGTCTG
    AGCCCCCTCC TGCAGAGAAG CCATCCCCCT GGGACCCTCC CCCCACCACG CCTCACACCC
    CCCGCCACAC CTGGCCCAGC CTGGCCTCCG CGGCCTGTGC CCACCCTGCG GCTGGAAGGG
    GCCGAGTCCA GCGACAAGCT CACCAGCAGC TTCCCATCCA TCCACTGCGA CCCCCACATC
    GGGGAGCCCA CGCCCTGCGG GGTTGTCGGC ACCCCCCGGA GGGCCCGGCC CGTGTCACTC
    ACTGTGCCCA GCCCGGCAGG GCCCCAGGGC CGTCCGTTCC ATGGCAGTGC CAGCAGCCTG
    GTGGAGGCGG TCTTGATCTC GGAAGGATTG GGGCAGTTTG CTCAGGACCC CAGGTTCCTC
    GAGGCCACCA CGCAGGAGCT GGCCGATGCC TGCGACATGA CCATTGAGGA GATGGAGAGC
    GCGGCTGACG ACATCCTCAG CGGGGGCGCC GGGCAGAGCC CCAACGGCAC CCTGCTCCCG
    TGTGCAAACT GCAGGGACCC GGGGCCGGAC CGAGCGGGGG GTGTGGAGGA CGCAGCCTGG
    GCGCCCAGCG CCGAGCCCCG CCAGGGCGCA GAGGAGCCCC GGGACAGCAG GGCCTTCGCC
    AGCGGCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848524.1
    CDS1836..8327
    Translation

    Target ORF information:

    RefSeq Version XM_024992756.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992756.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC
    TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC
    TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT
    GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC
    GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT
    GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC
    AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG
    GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC
    CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC
    ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC
    CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG
    AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC
    CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC
    CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG
    TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC
    GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC
    ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC
    TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG
    CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG
    AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC
    CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG
    ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC
    CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC
    CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG
    AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC
    GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC
    CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC
    TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG
    GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG
    GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC
    ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC
    TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT
    TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC
    GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG
    GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG
    ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG
    AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC
    ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC
    TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA
    TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG
    TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG
    GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA
    TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC
    TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG
    GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG
    GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG
    GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC
    CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC
    ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC
    AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT
    GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG
    AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT
    CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG
    TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC
    GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG
    AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG
    GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA
    GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC
    TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC
    ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC
    GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG
    GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT
    CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT
    GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA
    GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG
    ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG
    GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA
    GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGCTGGCCTG
    CGCACCCTGC ACGATCTGGG GCCCGAGATC CGCCGGGCCA TCTCTGGGGA CCTGACCGCC
    GAGGAGGAGC TGGACAAGGC CATGAAGGAG GCTGTGTCTG CAGCCTCAGA AGATGACATC
    TTCAGGAGGG CCGGCGGCCT GTTCGGCAAC CACGTGGGCT ACTACCCGAG CGACGGCCGG
    GGCGCCTTCC CCCAGACCTT CACCACCCAG CGCCCCCTGC ACATCAGCAA GGCAGGCAAC
    AGCCAGGGGG ACACCGAGTC CCCCTCCCAC GAGAAACTGG TGGACTCCAC CTTCACCCCC
    AGCAGCTACT CATCCACGGG CTCCAACGCC AACATCAACA ACGCCAACAA CACCGCCCTG
    GGCCGCTGCC CCCGCCCCGC GCTCCACTCC GGTGCTGTCA GCACCTTGGA GGCCCACGGG
    CCCCCCTTGT CCCCTGCCAT CCGGGTGCAG GAGGCCGCGT GGAGGCACAG CTCCCAGAGG
    TGCCCCTCCC GGGACAGCCC GATAGCCATG CTGTGTCAGG AAGAGGCCTC TGGGGACGAG
    ACCTGTGCCG TGCACGTGGA CGAGAACGCA GAGCACAGCA GCGAGCCCAG CTTGGTCTCC
    ACGGAGATGC TCTCCTACCA GGATGACGAG AATCGGCAGC TGACTCCCCC CGAGGAGGAC
    AGGAGGGACA TCCGTCTGTC CCCAAAGAGG GGCTTCCTGC GCTCCGCCTC CCTAGGTCGA
    AGGGCCTCCT TCCACCTTGA ATGTCTGAAG CGACAGAAGA ATCCTGGGGG AGACGTCTCT
    CAGAACACAG TCCTGCCTTT GCACCTGGTT CACCATCAGG CGCTGGCAGT GGCGGGTCTG
    AGCCCCCTCC TGCAGAGAAG CCATCCCCCT GGGACCCTCC CCCCACCACG CCTCACACCC
    CCCGCCACAC CTGGCCCAGC CTGGCCTCCG CGGCCTGTGC CCACCCTGCG GCTGGAAGGG
    GCCGAGTCCA GCGACAAGCT CACCAGCAGC TTCCCATCCA TCCACTGCGA CCCCCACATC
    GGGGAGCCCA CGCCCTGCGG GGTTGTCGGC ACCCCCCGGA GGGCCCGGCC CGTGTCACTC
    ACTGTGCCCA GCCCGGCAGG GCCCCAGGGC CGTCCGTTCC ATGGCAGTGC CAGCAGCCTG
    GTGGAGGCGG TCTTGATCTC GGAAGGATTG GGGCAGTTTG CTCAGGACCC CAGGTTCCTC
    GAGGCCACCA CGCAGGAGCT GGCCGATGCC TGCGACATGA CCATTGAGGA GATGGAGAGC
    GCGGCTGACG ACATCCTCAG CGGGGGCGCC GGGCAGAGCC CCAACGGCAC CCTGCTCCCG
    TGTGCAAACT GCAGGGACCC GGGGCCGGAC CGAGCGGGGG GTGTGGAGGA CGCAGCCTGG
    GCGCCCAGCG CCGAGCCCCG CCAGGGCGCA GAGGAGCCCC GGGACAGCAG GGCCTTCGCC
    AGCGGCCTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234943
    Clone ID Related Accession (Same CDS sequence) XM_024992757.1
    Accession Version XM_024992757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848525.1
    CDS1835..8305
    Translation

    Target ORF information:

    RefSeq Version XM_024992757.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234944
    Clone ID Related Accession (Same CDS sequence) XM_024992758.1
    Accession Version XM_024992758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848526.1
    CDS1836..8306
    Translation

    Target ORF information:

    RefSeq Version XM_024992758.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC
    GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234945
    Clone ID Related Accession (Same CDS sequence) XM_024992759.1
    Accession Version XM_024992759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848527.1
    CDS1836..8306
    Translation

    Target ORF information:

    RefSeq Version XM_024992759.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234946
    Clone ID Related Accession (Same CDS sequence) XM_024992760.1
    Accession Version XM_024992760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848528.1
    CDS1837..8307
    Translation

    Target ORF information:

    RefSeq Version XM_024992760.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC
    ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC
    GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT
    GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT
    GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC
    CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG
    CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG
    GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC
    TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG
    ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA
    GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA
    CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC
    CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG
    TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT
    GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC
    CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC
    CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG
    CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG
    CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC
    ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG
    TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC
    ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC
    CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC
    TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG
    CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC
    CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG
    ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC
    GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG
    GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC
    CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA
    TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG
    CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC
    CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC
    TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC
    ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG
    GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG
    CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG
    GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG
    GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC
    GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG
    GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC
    CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234947
    Clone ID Related Accession (Same CDS sequence) XM_024992761.1
    Accession Version XM_024992761.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6465bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGCTGGC CTGCGCACCC TGCACGATCT GGGGCCCGAG
    ATCCGCCGGG CCATCTCTGG GGACCTGACC GCCGAGGAGG AGCTGGACAA GGCCATGAAG
    GAGGCTGTGT CTGCAGCCTC AGAAGATGAC ATCTTCAGGA GGGCCGGCGG CCTGTTCGGC
    AACCACGTGG GCTACTACCC GAGCGACGGC CGGGGCGCCT TCCCCCAGAC CTTCACCACC
    CAGCGCCCCC TGCACATCAG CAAGGCAGGC AACAGCCAGG GGGACACCGA GTCCCCCTCC
    CACGAGAAAC TGGTGGACTC CACCTTCACC CCCAGCAGCT ACTCATCCAC GGGCTCCAAC
    GCCAACATCA ACAACGCCAA CAACACCGCC CTGGGCCGCT GCCCCCGCCC CGCGCTCCAC
    TCCGGTGCTG TCAGCACCTT GGAGGCCCAC GGGCCCCCCT TGTCCCCTGC CATCCGGGTG
    CAGGAGGCCG CGTGGAGGCA CAGCTCCCAG AGGTGCCCCT CCCGGGACAG CCCGATAGCC
    ATGCTGTGTC AGGAAGAGGC CTCTGGGGAC GAGACCTGTG CCGTGCACGT GGACGAGAAC
    GCAGAGCACA GCAGCGAGCC CAGCTTGGTC TCCACGGAGA TGCTCTCCTA CCAGGATGAC
    GAGAATCGGC AGCTGACTCC CCCCGAGGAG GACAGGAGGG ACATCCGTCT GTCCCCAAAG
    AGGGGCTTCC TGCGCTCCGC CTCCCTAGGT CGAAGGGCCT CCTTCCACCT TGAATGTCTG
    AAGCGACAGA AGAATCCTGG GGGAGACGTC TCTCAGAACA CAGTCCTGCC TTTGCACCTG
    GTTCACCATC AGGCGCTGGC AGTGGCGGGT CTGAGCCCCC TCCTGCAGAG AAGCCATCCC
    CCTGGGACCC TCCCCCCACC ACGCCTCACA CCCCCCGCCA CACCTGGCCC AGCCTGGCCT
    CCGCGGCCTG TGCCCACCCT GCGGCTGGAA GGGGCCGAGT CCAGCGACAA GCTCACCAGC
    AGCTTCCCAT CCATCCACTG CGACCCCCAC ATCGGGGAGC CCACGCCCTG CGGGGTTGTC
    GGCACCCCCC GGAGGGCCCG GCCCGTGTCA CTCACTGTGC CCAGCCCGGC AGGGCCCCAG
    GGCCGTCCGT TCCATGGCAG TGCCAGCAGC CTGGTGGAGG CGGTCTTGAT CTCGGAAGGA
    TTGGGGCAGT TTGCTCAGGA CCCCAGGTTC CTCGAGGCCA CCACGCAGGA GCTGGCCGAT
    GCCTGCGACA TGACCATTGA GGAGATGGAG AGCGCGGCTG ACGACATCCT CAGCGGGGGC
    GCCGGGCAGA GCCCCAACGG CACCCTGCTC CCGTGTGCAA ACTGCAGGGA CCCGGGGCCG
    GACCGAGCGG GGGGTGTGGA GGACGCAGCC TGGGCGCCCA GCGCCGAGCC CCGCCAGGGC
    GCAGAGGAGC CCCGGGACAG CAGGGCCTTC GCCAGCGGCC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848529.1
    CDS1835..8299
    Translation

    Target ORF information:

    RefSeq Version XM_024992761.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992761.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT
    GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC
    ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG
    GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC
    CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA
    ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG
    GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC
    TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG
    ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC
    TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG
    GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT
    GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT
    CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC
    TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC
    CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG
    CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG
    GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC
    CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC
    CAGAGGAACG CCCTGTCCCT GCAGGCTGGC CTGCGCACCC TGCACGATCT GGGGCCCGAG
    ATCCGCCGGG CCATCTCTGG GGACCTGACC GCCGAGGAGG AGCTGGACAA GGCCATGAAG
    GAGGCTGTGT CTGCAGCCTC AGAAGATGAC ATCTTCAGGA GGGCCGGCGG CCTGTTCGGC
    AACCACGTGG GCTACTACCC GAGCGACGGC CGGGGCGCCT TCCCCCAGAC CTTCACCACC
    CAGCGCCCCC TGCACATCAG CAAGGCAGGC AACAGCCAGG GGGACACCGA GTCCCCCTCC
    CACGAGAAAC TGGTGGACTC CACCTTCACC CCCAGCAGCT ACTCATCCAC GGGCTCCAAC
    GCCAACATCA ACAACGCCAA CAACACCGCC CTGGGCCGCT GCCCCCGCCC CGCGCTCCAC
    TCCGGTGCTG TCAGCACCTT GGAGGCCCAC GGGCCCCCCT TGTCCCCTGC CATCCGGGTG
    CAGGAGGCCG CGTGGAGGCA CAGCTCCCAG AGGTGCCCCT CCCGGGACAG CCCGATAGCC
    ATGCTGTGTC AGGAAGAGGC CTCTGGGGAC GAGACCTGTG CCGTGCACGT GGACGAGAAC
    GCAGAGCACA GCAGCGAGCC CAGCTTGGTC TCCACGGAGA TGCTCTCCTA CCAGGATGAC
    GAGAATCGGC AGCTGACTCC CCCCGAGGAG GACAGGAGGG ACATCCGTCT GTCCCCAAAG
    AGGGGCTTCC TGCGCTCCGC CTCCCTAGGT CGAAGGGCCT CCTTCCACCT TGAATGTCTG
    AAGCGACAGA AGAATCCTGG GGGAGACGTC TCTCAGAACA CAGTCCTGCC TTTGCACCTG
    GTTCACCATC AGGCGCTGGC AGTGGCGGGT CTGAGCCCCC TCCTGCAGAG AAGCCATCCC
    CCTGGGACCC TCCCCCCACC ACGCCTCACA CCCCCCGCCA CACCTGGCCC AGCCTGGCCT
    CCGCGGCCTG TGCCCACCCT GCGGCTGGAA GGGGCCGAGT CCAGCGACAA GCTCACCAGC
    AGCTTCCCAT CCATCCACTG CGACCCCCAC ATCGGGGAGC CCACGCCCTG CGGGGTTGTC
    GGCACCCCCC GGAGGGCCCG GCCCGTGTCA CTCACTGTGC CCAGCCCGGC AGGGCCCCAG
    GGCCGTCCGT TCCATGGCAG TGCCAGCAGC CTGGTGGAGG CGGTCTTGAT CTCGGAAGGA
    TTGGGGCAGT TTGCTCAGGA CCCCAGGTTC CTCGAGGCCA CCACGCAGGA GCTGGCCGAT
    GCCTGCGACA TGACCATTGA GGAGATGGAG AGCGCGGCTG ACGACATCCT CAGCGGGGGC
    GCCGGGCAGA GCCCCAACGG CACCCTGCTC CCGTGTGCAA ACTGCAGGGA CCCGGGGCCG
    GACCGAGCGG GGGGTGTGGA GGACGCAGCC TGGGCGCCCA GCGCCGAGCC CCGCCAGGGC
    GCAGAGGAGC CCCGGGACAG CAGGGCCTTC GCCAGCGGCC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234948
    Clone ID Related Accession (Same CDS sequence) XM_024992763.1
    Accession Version XM_024992763.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6417bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTCAATG AAAACACGAG GATGTACATT CCGGAGGAGA ACCACCAAGG TCCCAGCTAT 
    GGGAGCCCGC GCCCGGCTCA CGCCAACATG AACGCCAACG CGGCCGCGGG GCTGGCACCT
    GAGCACATCC CCACACCGGG GGCCGCCCTG TCCTGGCAGG CCGCCATCGA CGCGGCCCGG
    CAGGCCAAGC TGATGGGCAG TGCTGGCAAC GCAACCATCT CCACGGTCAG CTCCACGCAG
    CGGAAACGGC AGCAGTATGG GAAGCCCAAG AAGCAGGGCA GCACGACAGC CACCCGCCCG
    CCCCGCGCCC TGCTCTGCCT GACCCTAAAG AACCCCATCC GGAGGGCCTG CATCAGCATC
    GTCGAGTGGA AGCCATTTGA AATCATTATT TTACTGACTA TTTTTGCCAA TTGTGTGGCC
    TTAGCGATCT ATATTCCCTT CCCAGAGGAC GACTCCAACG CCACGAATTC CAACCTGGAG
    CGGGTGGAGT ATCTCTTCCT CATCATTTTT ACTGTGGAAG CATTTTTAAA AGTCATAGCC
    TACGGACTCC TCTTTCACCC CAACGCTTAC CTCCGCAATG GCTGGAACTT ACTAGACTTT
    ATCATCGTGG TTGTCGGGCT TTTCAGCGCG ATCTTAGAGC AAGCGACCAA AGCCGATGGG
    GCCAACGCCC TGGGAGGGAA GGGTGCTGGA TTCGACGTAA AGGCGCTGAG GGCGTTCCGT
    GTGCTACGGC CCCTGCGGTT GGTGTCCGGG GTCCCGAGTC TCCAGGTGGT CTTGAATTCC
    ATCATCAAGG CCATGGTGCC CCTGCTGCAC ATCGCCCTGC TCGTGCTGTT CGTCATCATC
    ATCTATGCCA TCATCGGCCT GGAGCTCTTC ATGGGCAAGA TGCATAAGAC CTGCTACAAC
    CAGGAGGGCA TTGCAGATGT CCCAGCAGAA GACGACCCCT CGCCCTGTGC CCTGGAGACA
    GGCCACGGGC GGCAGTGCCA GAATGGCACG GTGTGCAAAC CAGGCTGGGA TGGACCCAAG
    CACGGCATCA CCAATTTCGA CAACTTCGCC TTCGCCATGC TGACTGTGTT CCAGTGCATC
    ACCATGGAGG GCTGGACCGA CGTCCTCTAC TGGGTCAATG ATGCCGTGGG AAGGGACTGG
    CCCTGGATCT ATTTTGTCAC ATTAATCATC ATAGGTTCAT TCTTTGTTCT TAACTTGGTT
    CTCGGTGTCC TTAGCGGAGA GTTCTCCAAA GAGCGAGAGA AGGCCAAGGC CCGGGGAGAC
    TTCCAGAAGC TGCGAGAGAA GCAGCAGCTG GAGGAGGACC TGAAGGGCTA CCTGGACTGG
    ATCACGCAGG CCGAGGACAT CGACCCCGAG AATGAGGACG AGGGCCTGGA CGAGGAGAAA
    CCCCGAAACA TGAGCATGCC CACCAGTGAG ACCGAGTCTG TCAACACCGA AAACGTGGCT
    GGAGGGGACA TCGAGGGAGA GAGCTGCGGG GCCAGGCTGG CCCACCGGAT CTCCAAGTCA
    AAGTTCAGCC GCTACTGGCG CCGGTGGAAT CGGTTCTGCA GGAGGAAGTG CCGCGCGGCC
    GTGAAGTCCA GCATCTTCTA CTGGCTGGTC ATCTTCCTGG TTTTCCTCAA CACACTCACC
    ATCGCCTCCG AGCACTACAA CCAGCCCCAC TGGCTCACGG AAGTCCAAGA CACGGCCAAC
    AAGGCGCTGC TGGCCCTGTT CACGGCGGAG ATGCTGCTCA AGATGTACAG CCTGGGCCTG
    CAGGCCTACT TCGTGTCGCT CTTCAACCGC TTCGACTGCT TCATCGTGTG CGGGGGCATC
    CTGGAGACCA TCCTGGTGGA GACCAAGATC ATGTCGCCGT TGGGCATCTC CGTGCTGCGT
    TGCGTGCGCC TCCTTCGGAT ATTTAAGATC ACAAGGTACT GGAACTCCCT GAGCAACCTG
    GTGGCATCCC TGCTGAACTC AGTGCGCTCC ATTGCCTCGC TGCTGCTGCT GCTCTTCCTC
    TTCATCATCA TCTTCTCCCT CCTGGGGATG CAGCTCTTTG GGGGGAAGTT CAACTTTGAT
    GAGATGCAGA CCCGGAGGAG CACCTTTGAC AACTTCCCGC AGTCCCTCCT CACCGTGTTT
    CAGATCCTGA CCGGGGAGGA CTGGAATTCG GTGATGTATG ATGGGATCAT GGCTTATGGC
    GGCCCCTCTT TTCCAGGGAT GTTAGTCTGT ATTTACTTCA TCATCCTCTT CATCTGTGGA
    AATTATATCC TACTGAACGT GTTCTTGGCC ATTGCTGTGG ACAACCTGGC TGACGCGGAG
    AGCCTCACAT CTGCCCAGAA GGAGGAGGAA GAGGAGAAGG AGAGGAAGAA GCTAGCCAGG
    ACCGCCAGCC CAGAGAAGAA GCAAGAGGTG GTAGAGAAGC CGGCCGTGGA GGAGACCAAG
    GAGGAGAAAA TCGAGCTGAA ATCCATCACG GCAGATGGAG AGTCCCCACC CGCCACCAAG
    ATCAACATGG ATGACCTCCA GCCCAACGAG AATGAGGACA AGAGCTCCTA CCCCAACCAG
    GAAACCACAG GAGAAGAGGA CGAGGAGGAG CCCGAGATGC CCGTGGGCCC CCGCCCGCGC
    CCCCTCTCTG AGCTGCACCT GAAGGAGAAG GCAGTGCCCA TGCCGGAAGC CAGCGCCTTC
    TTCATCTTCA GCCCCAGCAA CAGGTTCCGC CTCCAGTGCC ATCGCATCGT CAATGACTCC
    ATCTTCACCA ACCTGATCCT CTTCTTCATC CTGCTCAGCA GCATCTCCCT GGCCGCCGAG
    GACCCCGTGC AGCACACCTC CTTCAGGAAC CACATTCTGT TTTATTTTGA CATTGTTTTC
    ACTACCATTT TCACCATTGA GATTGCCCTG AAGATGACGG CCTACGGGGC CTTCCTGCAC
    AAGGGCTCCT TCTGCCGCAA CTACTTCAAC ATCCTGGACC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TCGGCATCCA GTCCAGTGCC ATCAACGTGG TGAAGATCCT GCGAGTCCTG
    AGGGTGCTCC GGCCACTGAG GGCCATCAAC AGGGCCAAGG GGCTGAAGCA CGTCGTGCAG
    TGCGTGTTCG TGGCCATCCG CACCATCGGG AACATTGTGA TCGTCACCAC GCTGCTCCAG
    TTCATGTTCG CCTGCATTGG GGTCCAGCTC TTCAAGGGAA AGCTCTACAC CTGTTCGGAC
    AGTTCCAAAC AGACAGAGGC TGAATGCAAG GGGAACTACA TAACATACAA GGATGGGGAG
    GTGGACCACC CCATCATCCA GCCCCGCAGC TGGGAGAACA GCAAGTTTGA CTTTGACAAT
    GTCCTGGCAG CCATGATGGC CCTGTTCACC GTCTCCACCT TCGAGGGATG GCCAGAGTTG
    CTGTACCGCT CCATCGACTC TCACACGGAG GACAAGGGTC CCATATACAA CTACCGCGTG
    GAGATCTCCG TCTTCTTCAT CATCTACATC ATCATCATTG CCTTCTTCAT GATGAACATC
    TTCGTGGGTT TCGTCATCGT CACCTTCCAG GAGCAGGGGG AGCAGGAATA CAAGAACTGC
    GAGCTGGACA AGAACCAGCG GCAGTGTGTG GAGTATGCCC TCAAGGCCCG GCCCCTGCGG
    AGGTACATCC CGAAAAACCA GCACCAGTAC AAGGTGTGGT ACGTGGTCAA CTCCACCTAC
    TTCGAGTACC TGATGTTCGT TCTCATTCTG CTCAACACCA TCTGCCTGGC CATGCAGCAC
    TACGGTCAGA GCTGTCTGTT CAAAATCGCC ATGAACATCC TCAACATGCT CTTCACCGGC
    CTCTTCACAG TGGAGATGGT CCTGAAGCTC ATCGCCTTCA AGCCCAAGCA CTATTTCTGT
    GATGCATGGA ACACATTTGA CGCCTTGATT GTTGTGGGTA GCATTGTTGA TATAGCCATC
    ACCGAGGTAA ACCCAGCTGA ACATACCCAA TGCTCTCCCT TTATGAACGC GGAAGAGAAC
    TCTCGCATCT CCATCACCTT CTTCCGCCTC TTCCGGGTCA TGCGTCTGGT CAAGCTGCTG
    AGCCGCGGGG AGGGCATCCG GACACTGCTG TGGACCTTCA TCAAGTCCTT CCAGGCCCTA
    CCCTATGTGG CCCTCCTGAT CGTGATGCTG TTTTTCATCT ACGCCGTGAT CGGGATGCAG
    GTGTTTGGCA AAATTGCCCT GAATGACACG ACAGAGATCA ACAGGAACAA CAACTTCCAG
    ACCTTCCCCC AGGCCGTGCT GCTCCTCTTC AGGTGCGCCA CTGGGGAGGC CTGGCAGGAC
    ATCATGCTGG CCTGCATGCC TGGCAAGAAG TGCGCCCCCG AGTCAGAGCC GGGCAACAGC
    ACAGAGGGGG AGACGCCCTG TGGCAGCAGC TTCGCCGTCT TCTACTTCAT CAGCTTCTAC
    ATGCTCTGCG CCTTCCTGAT CATCAACCTC TTCGTAGCCG TCATCATGGA CAACTTTGAC
    TACCTGACGA GGGACTGGTC CATCCTGGGT CCCCACCACC TGGACGAATT TAAGAGAATT
    TGGGCAGAGT ATGACCCTGA AGCCAAGGGA CGCATCAAAC ACCTGGATGT GGTGACCCTG
    CTCCGACGGA TTCAGCCCCC CCTGGGTTTT GGGAAGCTGT GCCCTCACCG CGTGGCTTGC
    AAACGCCTGG TCTCCATGAA CATGCCCCTG AACAGCGACG GGACTGTCAT GTTCAATGCC
    ACGCTGTTTG CCCTGGTCCG TACAGCTCTG AGGATCAAGA CGGAAGGGAA CCTGGAACAG
    GCCAATGAGG AGCTGCGGGC CATCATCAAG AAGATCTGGA AACGGACCAG CATGAAGCTG
    CTGGACCAAG TGGTGCCGCC CGCAGGAGAT GACGAGGTCA CGGTGGGCAA GTTTTACGCT
    ACTTTCCTGA TCCAGGAGTA TTTCCGGAAG TTCAAGAAGC GCAAAGAGCA AGGCCTGGTG
    GGCAAGCCCT CCCAGAGGAA CGCCCTGTCC CTGCAGGCTG GCCTGCGCAC CCTGCACGAT
    CTGGGGCCCG AGATCCGCCG GGCCATCTCT GGGGACCTGA CCGCCGAGGA GGAGCTGGAC
    AAGGCCATGA AGGAGGCTGT GTCTGCAGCC TCAGAAGATG ACATCTTCAG GAGGGCCGGC
    GGCCTGTTCG GCAACCACGT GGGCTACTAC CCGAGCGACG GCCGGGGCGC CTTCCCCCAG
    ACCTTCACCA CCCAGCGCCC CCTGCACATC AGCAAGGCAG GCAACAGCCA GGGGGACACC
    GAGTCCCCCT CCCACGAGAA ACTGGTGGAC TCCACCTTCA CCCCCAGCAG CTACTCATCC
    ACGGGCTCCA ACGCCAACAT CAACAACGCC AACAACACCG CCCTGGGCCG CTGCCCCCGC
    CCCGCGCTCC ACTCCGGTGC TGTCAGCACC TTGGAGGCCC ACGGGCCCCC CTTGTCCCCT
    GCCATCCGGG TGCAGGAGGC CGCGTGGAGG CACAGCTCCC AGAGGTGCCC CTCCCGGGAC
    AGCCCGATAG CCATGCTGTG TCAGGAAGAG GCCTCTGGGG ACGAGACCTG TGCCGTGCAC
    GTGGACGAGA ACGCAGAGCA CAGCAGCGAG CCCAGCTTGG TCTCCACGGA GATGCTCTCC
    TACCAGGATG ACGAGAATCG GCAGCTGACT CCCCCCGAGG AGGACAGGAG GGACATCCGT
    CTGTCCCCAA AGAGGGGCTT CCTGCGCTCC GCCTCCCTAG GTCGAAGGGC CTCCTTCCAC
    CTTGAATGTC TGAAGCGACA GAAGAATCCT GGGGGAGACG TCTCTCAGAA CACAGTCCTG
    CCTTTGCACC TGGTTCACCA TCAGGCGCTG GCAGTGGCGG GTCTGAGCCC CCTCCTGCAG
    AGAAGCCATC CCCCTGGGAC CCTCCCCCCA CCACGCCTCA CACCCCCCGC CACACCTGGC
    CCAGCCTGGC CTCCGCGGCC TGTGCCCACC CTGCGGCTGG AAGGGGCCGA GTCCAGCGAC
    AAGCTCACCA GCAGCTTCCC ATCCATCCAC TGCGACCCCC ACATCGGGGA GCCCACGCCC
    TGCGGGGTTG TCGGCACCCC CCGGAGGGCC CGGCCCGTGT CACTCACTGT GCCCAGCCCG
    GCAGGGCCCC AGGGCCGTCC GTTCCATGGC AGTGCCAGCA GCCTGGTGGA GGCGGTCTTG
    ATCTCGGAAG GATTGGGGCA GTTTGCTCAG GACCCCAGGT TCCTCGAGGC CACCACGCAG
    GAGCTGGCCG ATGCCTGCGA CATGACCATT GAGGAGATGG AGAGCGCGGC TGACGACATC
    CTCAGCGGGG GCGCCGGGCA GAGCCCCAAC GGCACCCTGC TCCCGTGTGC AAACTGCAGG
    GACCCGGGGC CGGACCGAGC GGGGGGTGTG GAGGACGCAG CCTGGGCGCC CAGCGCCGAG
    CCCCGCCAGG GCGCAGAGGA GCCCCGGGAC AGCAGGGCCT TCGCCAGCGG CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848531.1
    CDS556..6972
    Translation

    Target ORF information:

    RefSeq Version XM_024992763.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992763.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGTCAATG AAAACACGAG GATGTACATT CCGGAGGAGA ACCACCAAGG TCCCAGCTAT 
    GGGAGCCCGC GCCCGGCTCA CGCCAACATG AACGCCAACG CGGCCGCGGG GCTGGCACCT
    GAGCACATCC CCACACCGGG GGCCGCCCTG TCCTGGCAGG CCGCCATCGA CGCGGCCCGG
    CAGGCCAAGC TGATGGGCAG TGCTGGCAAC GCAACCATCT CCACGGTCAG CTCCACGCAG
    CGGAAACGGC AGCAGTATGG GAAGCCCAAG AAGCAGGGCA GCACGACAGC CACCCGCCCG
    CCCCGCGCCC TGCTCTGCCT GACCCTAAAG AACCCCATCC GGAGGGCCTG CATCAGCATC
    GTCGAGTGGA AGCCATTTGA AATCATTATT TTACTGACTA TTTTTGCCAA TTGTGTGGCC
    TTAGCGATCT ATATTCCCTT CCCAGAGGAC GACTCCAACG CCACGAATTC CAACCTGGAG
    CGGGTGGAGT ATCTCTTCCT CATCATTTTT ACTGTGGAAG CATTTTTAAA AGTCATAGCC
    TACGGACTCC TCTTTCACCC CAACGCTTAC CTCCGCAATG GCTGGAACTT ACTAGACTTT
    ATCATCGTGG TTGTCGGGCT TTTCAGCGCG ATCTTAGAGC AAGCGACCAA AGCCGATGGG
    GCCAACGCCC TGGGAGGGAA GGGTGCTGGA TTCGACGTAA AGGCGCTGAG GGCGTTCCGT
    GTGCTACGGC CCCTGCGGTT GGTGTCCGGG GTCCCGAGTC TCCAGGTGGT CTTGAATTCC
    ATCATCAAGG CCATGGTGCC CCTGCTGCAC ATCGCCCTGC TCGTGCTGTT CGTCATCATC
    ATCTATGCCA TCATCGGCCT GGAGCTCTTC ATGGGCAAGA TGCATAAGAC CTGCTACAAC
    CAGGAGGGCA TTGCAGATGT CCCAGCAGAA GACGACCCCT CGCCCTGTGC CCTGGAGACA
    GGCCACGGGC GGCAGTGCCA GAATGGCACG GTGTGCAAAC CAGGCTGGGA TGGACCCAAG
    CACGGCATCA CCAATTTCGA CAACTTCGCC TTCGCCATGC TGACTGTGTT CCAGTGCATC
    ACCATGGAGG GCTGGACCGA CGTCCTCTAC TGGGTCAATG ATGCCGTGGG AAGGGACTGG
    CCCTGGATCT ATTTTGTCAC ATTAATCATC ATAGGTTCAT TCTTTGTTCT TAACTTGGTT
    CTCGGTGTCC TTAGCGGAGA GTTCTCCAAA GAGCGAGAGA AGGCCAAGGC CCGGGGAGAC
    TTCCAGAAGC TGCGAGAGAA GCAGCAGCTG GAGGAGGACC TGAAGGGCTA CCTGGACTGG
    ATCACGCAGG CCGAGGACAT CGACCCCGAG AATGAGGACG AGGGCCTGGA CGAGGAGAAA
    CCCCGAAACA TGAGCATGCC CACCAGTGAG ACCGAGTCTG TCAACACCGA AAACGTGGCT
    GGAGGGGACA TCGAGGGAGA GAGCTGCGGG GCCAGGCTGG CCCACCGGAT CTCCAAGTCA
    AAGTTCAGCC GCTACTGGCG CCGGTGGAAT CGGTTCTGCA GGAGGAAGTG CCGCGCGGCC
    GTGAAGTCCA GCATCTTCTA CTGGCTGGTC ATCTTCCTGG TTTTCCTCAA CACACTCACC
    ATCGCCTCCG AGCACTACAA CCAGCCCCAC TGGCTCACGG AAGTCCAAGA CACGGCCAAC
    AAGGCGCTGC TGGCCCTGTT CACGGCGGAG ATGCTGCTCA AGATGTACAG CCTGGGCCTG
    CAGGCCTACT TCGTGTCGCT CTTCAACCGC TTCGACTGCT TCATCGTGTG CGGGGGCATC
    CTGGAGACCA TCCTGGTGGA GACCAAGATC ATGTCGCCGT TGGGCATCTC CGTGCTGCGT
    TGCGTGCGCC TCCTTCGGAT ATTTAAGATC ACAAGGTACT GGAACTCCCT GAGCAACCTG
    GTGGCATCCC TGCTGAACTC AGTGCGCTCC ATTGCCTCGC TGCTGCTGCT GCTCTTCCTC
    TTCATCATCA TCTTCTCCCT CCTGGGGATG CAGCTCTTTG GGGGGAAGTT CAACTTTGAT
    GAGATGCAGA CCCGGAGGAG CACCTTTGAC AACTTCCCGC AGTCCCTCCT CACCGTGTTT
    CAGATCCTGA CCGGGGAGGA CTGGAATTCG GTGATGTATG ATGGGATCAT GGCTTATGGC
    GGCCCCTCTT TTCCAGGGAT GTTAGTCTGT ATTTACTTCA TCATCCTCTT CATCTGTGGA
    AATTATATCC TACTGAACGT GTTCTTGGCC ATTGCTGTGG ACAACCTGGC TGACGCGGAG
    AGCCTCACAT CTGCCCAGAA GGAGGAGGAA GAGGAGAAGG AGAGGAAGAA GCTAGCCAGG
    ACCGCCAGCC CAGAGAAGAA GCAAGAGGTG GTAGAGAAGC CGGCCGTGGA GGAGACCAAG
    GAGGAGAAAA TCGAGCTGAA ATCCATCACG GCAGATGGAG AGTCCCCACC CGCCACCAAG
    ATCAACATGG ATGACCTCCA GCCCAACGAG AATGAGGACA AGAGCTCCTA CCCCAACCAG
    GAAACCACAG GAGAAGAGGA CGAGGAGGAG CCCGAGATGC CCGTGGGCCC CCGCCCGCGC
    CCCCTCTCTG AGCTGCACCT GAAGGAGAAG GCAGTGCCCA TGCCGGAAGC CAGCGCCTTC
    TTCATCTTCA GCCCCAGCAA CAGGTTCCGC CTCCAGTGCC ATCGCATCGT CAATGACTCC
    ATCTTCACCA ACCTGATCCT CTTCTTCATC CTGCTCAGCA GCATCTCCCT GGCCGCCGAG
    GACCCCGTGC AGCACACCTC CTTCAGGAAC CACATTCTGT TTTATTTTGA CATTGTTTTC
    ACTACCATTT TCACCATTGA GATTGCCCTG AAGATGACGG CCTACGGGGC CTTCCTGCAC
    AAGGGCTCCT TCTGCCGCAA CTACTTCAAC ATCCTGGACC TGCTGGTGGT CAGTGTGTCC
    CTCATCTCCT TCGGCATCCA GTCCAGTGCC ATCAACGTGG TGAAGATCCT GCGAGTCCTG
    AGGGTGCTCC GGCCACTGAG GGCCATCAAC AGGGCCAAGG GGCTGAAGCA CGTCGTGCAG
    TGCGTGTTCG TGGCCATCCG CACCATCGGG AACATTGTGA TCGTCACCAC GCTGCTCCAG
    TTCATGTTCG CCTGCATTGG GGTCCAGCTC TTCAAGGGAA AGCTCTACAC CTGTTCGGAC
    AGTTCCAAAC AGACAGAGGC TGAATGCAAG GGGAACTACA TAACATACAA GGATGGGGAG
    GTGGACCACC CCATCATCCA GCCCCGCAGC TGGGAGAACA GCAAGTTTGA CTTTGACAAT
    GTCCTGGCAG CCATGATGGC CCTGTTCACC GTCTCCACCT TCGAGGGATG GCCAGAGTTG
    CTGTACCGCT CCATCGACTC TCACACGGAG GACAAGGGTC CCATATACAA CTACCGCGTG
    GAGATCTCCG TCTTCTTCAT CATCTACATC ATCATCATTG CCTTCTTCAT GATGAACATC
    TTCGTGGGTT TCGTCATCGT CACCTTCCAG GAGCAGGGGG AGCAGGAATA CAAGAACTGC
    GAGCTGGACA AGAACCAGCG GCAGTGTGTG GAGTATGCCC TCAAGGCCCG GCCCCTGCGG
    AGGTACATCC CGAAAAACCA GCACCAGTAC AAGGTGTGGT ACGTGGTCAA CTCCACCTAC
    TTCGAGTACC TGATGTTCGT TCTCATTCTG CTCAACACCA TCTGCCTGGC CATGCAGCAC
    TACGGTCAGA GCTGTCTGTT CAAAATCGCC ATGAACATCC TCAACATGCT CTTCACCGGC
    CTCTTCACAG TGGAGATGGT CCTGAAGCTC ATCGCCTTCA AGCCCAAGCA CTATTTCTGT
    GATGCATGGA ACACATTTGA CGCCTTGATT GTTGTGGGTA GCATTGTTGA TATAGCCATC
    ACCGAGGTAA ACCCAGCTGA ACATACCCAA TGCTCTCCCT TTATGAACGC GGAAGAGAAC
    TCTCGCATCT CCATCACCTT CTTCCGCCTC TTCCGGGTCA TGCGTCTGGT CAAGCTGCTG
    AGCCGCGGGG AGGGCATCCG GACACTGCTG TGGACCTTCA TCAAGTCCTT CCAGGCCCTA
    CCCTATGTGG CCCTCCTGAT CGTGATGCTG TTTTTCATCT ACGCCGTGAT CGGGATGCAG
    GTGTTTGGCA AAATTGCCCT GAATGACACG ACAGAGATCA ACAGGAACAA CAACTTCCAG
    ACCTTCCCCC AGGCCGTGCT GCTCCTCTTC AGGTGCGCCA CTGGGGAGGC CTGGCAGGAC
    ATCATGCTGG CCTGCATGCC TGGCAAGAAG TGCGCCCCCG AGTCAGAGCC GGGCAACAGC
    ACAGAGGGGG AGACGCCCTG TGGCAGCAGC TTCGCCGTCT TCTACTTCAT CAGCTTCTAC
    ATGCTCTGCG CCTTCCTGAT CATCAACCTC TTCGTAGCCG TCATCATGGA CAACTTTGAC
    TACCTGACGA GGGACTGGTC CATCCTGGGT CCCCACCACC TGGACGAATT TAAGAGAATT
    TGGGCAGAGT ATGACCCTGA AGCCAAGGGA CGCATCAAAC ACCTGGATGT GGTGACCCTG
    CTCCGACGGA TTCAGCCCCC CCTGGGTTTT GGGAAGCTGT GCCCTCACCG CGTGGCTTGC
    AAACGCCTGG TCTCCATGAA CATGCCCCTG AACAGCGACG GGACTGTCAT GTTCAATGCC
    ACGCTGTTTG CCCTGGTCCG TACAGCTCTG AGGATCAAGA CGGAAGGGAA CCTGGAACAG
    GCCAATGAGG AGCTGCGGGC CATCATCAAG AAGATCTGGA AACGGACCAG CATGAAGCTG
    CTGGACCAAG TGGTGCCGCC CGCAGGAGAT GACGAGGTCA CGGTGGGCAA GTTTTACGCT
    ACTTTCCTGA TCCAGGAGTA TTTCCGGAAG TTCAAGAAGC GCAAAGAGCA AGGCCTGGTG
    GGCAAGCCCT CCCAGAGGAA CGCCCTGTCC CTGCAGGCTG GCCTGCGCAC CCTGCACGAT
    CTGGGGCCCG AGATCCGCCG GGCCATCTCT GGGGACCTGA CCGCCGAGGA GGAGCTGGAC
    AAGGCCATGA AGGAGGCTGT GTCTGCAGCC TCAGAAGATG ACATCTTCAG GAGGGCCGGC
    GGCCTGTTCG GCAACCACGT GGGCTACTAC CCGAGCGACG GCCGGGGCGC CTTCCCCCAG
    ACCTTCACCA CCCAGCGCCC CCTGCACATC AGCAAGGCAG GCAACAGCCA GGGGGACACC
    GAGTCCCCCT CCCACGAGAA ACTGGTGGAC TCCACCTTCA CCCCCAGCAG CTACTCATCC
    ACGGGCTCCA ACGCCAACAT CAACAACGCC AACAACACCG CCCTGGGCCG CTGCCCCCGC
    CCCGCGCTCC ACTCCGGTGC TGTCAGCACC TTGGAGGCCC ACGGGCCCCC CTTGTCCCCT
    GCCATCCGGG TGCAGGAGGC CGCGTGGAGG CACAGCTCCC AGAGGTGCCC CTCCCGGGAC
    AGCCCGATAG CCATGCTGTG TCAGGAAGAG GCCTCTGGGG ACGAGACCTG TGCCGTGCAC
    GTGGACGAGA ACGCAGAGCA CAGCAGCGAG CCCAGCTTGG TCTCCACGGA GATGCTCTCC
    TACCAGGATG ACGAGAATCG GCAGCTGACT CCCCCCGAGG AGGACAGGAG GGACATCCGT
    CTGTCCCCAA AGAGGGGCTT CCTGCGCTCC GCCTCCCTAG GTCGAAGGGC CTCCTTCCAC
    CTTGAATGTC TGAAGCGACA GAAGAATCCT GGGGGAGACG TCTCTCAGAA CACAGTCCTG
    CCTTTGCACC TGGTTCACCA TCAGGCGCTG GCAGTGGCGG GTCTGAGCCC CCTCCTGCAG
    AGAAGCCATC CCCCTGGGAC CCTCCCCCCA CCACGCCTCA CACCCCCCGC CACACCTGGC
    CCAGCCTGGC CTCCGCGGCC TGTGCCCACC CTGCGGCTGG AAGGGGCCGA GTCCAGCGAC
    AAGCTCACCA GCAGCTTCCC ATCCATCCAC TGCGACCCCC ACATCGGGGA GCCCACGCCC
    TGCGGGGTTG TCGGCACCCC CCGGAGGGCC CGGCCCGTGT CACTCACTGT GCCCAGCCCG
    GCAGGGCCCC AGGGCCGTCC GTTCCATGGC AGTGCCAGCA GCCTGGTGGA GGCGGTCTTG
    ATCTCGGAAG GATTGGGGCA GTTTGCTCAG GACCCCAGGT TCCTCGAGGC CACCACGCAG
    GAGCTGGCCG ATGCCTGCGA CATGACCATT GAGGAGATGG AGAGCGCGGC TGACGACATC
    CTCAGCGGGG GCGCCGGGCA GAGCCCCAAC GGCACCCTGC TCCCGTGTGC AAACTGCAGG
    GACCCGGGGC CGGACCGAGC GGGGGGTGTG GAGGACGCAG CCTGGGCGCC CAGCGCCGAG
    CCCCGCCAGG GCGCAGAGGA GCCCCGGGAC AGCAGGGCCT TCGCCAGCGG CCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa234949
    Clone ID Related Accession (Same CDS sequence) XM_024992764.1
    Accession Version XM_024992764.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6648bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC ACTATTTCTG TGATGCATGG
    AACACATTTG ACGCCTTGAT TGTTGTGGGT AGCATTGTTG ATATAGCCAT CACCGAGGTA
    AACCCAGCTG AACATACCCA ATGCTCTCCC TTTATGAACG CGGAAGAGAA CTCTCGCATC
    TCCATCACCT TCTTCCGCCT CTTCCGGGTC ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG
    GAGGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TCCAGGCCCT ACCCTATGTG
    GCCCTCCTGA TCGTGATGCT GTTTTTCATC TACGCCGTGA TCGGGATGCA GGTGTTTGGC
    AAAATTGCCC TGAATGACAC GACAGAGATC AACAGGAACA ACAACTTCCA GACCTTCCCC
    CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC ACTGGGGAGG CCTGGCAGGA CATCATGCTG
    GCCTGCATGC CTGGCAAGAA GTGCGCCCCC GAGTCAGAGC CGGGCAACAG CACAGAGGGG
    GAGACGCCCT GTGGCAGCAG CTTCGCCGTC TTCTACTTCA TCAGCTTCTA CATGCTCTGC
    GCCTTCCTGA TCATCAACCT CTTCGTAGCC GTCATCATGG ACAACTTTGA CTACCTGACG
    AGGGACTGGT CCATCCTGGG TCCCCACCAC CTGGACGAAT TTAAGAGAAT TTGGGCAGAG
    TATGACCCTG AAGCCAAGGG ACGCATCAAA CACCTGGATG TGGTGACCCT GCTCCGACGG
    ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG TGCCCTCACC GCGTGGCTTG CAAACGCCTG
    GTCTCCATGA ACATGCCCCT GAACAGCGAC GGGACTGTCA TGTTCAATGC CACGCTGTTT
    GCCCTGGTCC GTACAGCTCT GAGGATCAAG ACGGAAGGGA ACCTGGAACA GGCCAATGAG
    GAGCTGCGGG CCATCATCAA GAAGATCTGG AAACGGACCA GCATGAAGCT GCTGGACCAA
    GTGGTGCCGC CCGCAGGAGA TGACGAGGTC ACGGTGGGCA AGTTTTACGC TACTTTCCTG
    ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC
    TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT GGCCTGCGCA CCCTGCACGA TCTGGGGCCC
    GAGATCCGCC GGGCCATCTC TGGGGACCTG ACCGCCGAGG AGGAGCTGGA CAAGGCCATG
    AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT GACATCTTCA GGAGGGCCGG CGGCCTGTTC
    GGCAACCACG TGGGCTACTA CCCGAGCGAC GGCCGGGGCG CCTTCCCCCA GACCTTCACC
    ACCCAGCGCC CCCTGCACAT CAGCAAGGCA GGCAACAGCC AGGGGGACAC CGAGTCCCCC
    TCCCACGAGA AACTGGTGGA CTCCACCTTC ACCCCCAGCA GCTACTCATC CACGGGCTCC
    AACGCCAACA TCAACAACGC CAACAACACC GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC
    CACTCCGGTG CTGTCAGCAC CTTGGAGGCC CACGGGCCCC CCTTGTCCCC TGCCATCCGG
    GTGCAGGAGG CCGCGTGGAG GCACAGCTCC CAGAGGTGCC CCTCCCGGGA CAGCCCGATA
    GCCATGCTGT GTCAGGAAGA GGCCTCTGGG GACGAGACCT GTGCCGTGCA CGTGGACGAG
    AACGCAGAGC ACAGCAGCGA GCCCAGCTTG GTCTCCACGG AGATGCTCTC CTACCAGGAT
    GACGAGAATC GGCAGCTGAC TCCCCCCGAG GAGGACAGGA GGGACATCCG TCTGTCCCCA
    AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA GGTCGAAGGG CCTCCTTCCA CCTTGAATGT
    CTGAAGCGAC AGAAGAATCC TGGGGGAGAC GTCTCTCAGA ACACAGTCCT GCCTTTGCAC
    CTGGTTCACC ATCAGGCGCT GGCAGTGGCG GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT
    CCCCCTGGGA CCCTCCCCCC ACCACGCCTC ACACCCCCCG CCACACCTGG CCCAGCCTGG
    CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG GAAGGGGCCG AGTCCAGCGA CAAGCTCACC
    AGCAGCTTCC CATCCATCCA CTGCGACCCC CACATCGGGG AGCCCACGCC CTGCGGGGTT
    GTCGGCACCC CCCGGAGGGC CCGGCCCGTG TCACTCACTG TGCCCAGCCC GGCAGGGCCC
    CAGGGCCGTC CGTTCCATGG CAGTGCCAGC AGCCTGGTGG AGGCGGTCTT GATCTCGGAA
    GGATTGGGGC AGTTTGCTCA GGACCCCAGG TTCCTCGAGG CCACCACGCA GGAGCTGGCC
    GATGCCTGCG ACATGACCAT TGAGGAGATG GAGAGCGCGG CTGACGACAT CCTCAGCGGG
    GGCGCCGGGC AGAGCCCCAA CGGCACCCTG CTCCCGTGTG CAAACTGCAG GGACCCGGGG
    CCGGACCGAG CGGGGGGTGT GGAGGACGCA GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG
    GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848532.1
    CDS1839..8486
    Translation

    Target ORF information:

    RefSeq Version XM_024992764.1
    Organism Bos taurus(cattle)
    Definition Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024992764.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
    GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC
    TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC
    GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC
    TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA
    ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG
    CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC
    CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA
    CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC
    TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT
    GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC
    CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC
    TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC
    GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC
    CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC
    GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG
    GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC
    GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG
    TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC
    GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG
    GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA
    GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG
    CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG
    GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT
    GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC
    GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC
    AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG
    TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC
    TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG
    CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG
    CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC
    GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG
    TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA
    AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT
    GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG
    CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC
    TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG
    ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT
    TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT
    GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG
    GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA
    GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA
    GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT
    GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC
    GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA
    GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC
    CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG
    CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC
    ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG
    ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG
    ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC
    CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC
    AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG
    GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC
    ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC
    AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG
    AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG
    GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC
    TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC
    AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC
    ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG
    CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG
    TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG
    GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC
    AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG
    AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC
    GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA
    ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC ACTATTTCTG TGATGCATGG
    AACACATTTG ACGCCTTGAT TGTTGTGGGT AGCATTGTTG ATATAGCCAT CACCGAGGTA
    AACCCAGCTG AACATACCCA ATGCTCTCCC TTTATGAACG CGGAAGAGAA CTCTCGCATC
    TCCATCACCT TCTTCCGCCT CTTCCGGGTC ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG
    GAGGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TCCAGGCCCT ACCCTATGTG
    GCCCTCCTGA TCGTGATGCT GTTTTTCATC TACGCCGTGA TCGGGATGCA GGTGTTTGGC
    AAAATTGCCC TGAATGACAC GACAGAGATC AACAGGAACA ACAACTTCCA GACCTTCCCC
    CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC ACTGGGGAGG CCTGGCAGGA CATCATGCTG
    GCCTGCATGC CTGGCAAGAA GTGCGCCCCC GAGTCAGAGC CGGGCAACAG CACAGAGGGG
    GAGACGCCCT GTGGCAGCAG CTTCGCCGTC TTCTACTTCA TCAGCTTCTA CATGCTCTGC
    GCCTTCCTGA TCATCAACCT CTTCGTAGCC GTCATCATGG ACAACTTTGA CTACCTGACG
    AGGGACTGGT CCATCCTGGG TCCCCACCAC CTGGACGAAT TTAAGAGAAT TTGGGCAGAG
    TATGACCCTG AAGCCAAGGG ACGCATCAAA CACCTGGATG TGGTGACCCT GCTCCGACGG
    ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG TGCCCTCACC GCGTGGCTTG CAAACGCCTG
    GTCTCCATGA ACATGCCCCT GAACAGCGAC GGGACTGTCA TGTTCAATGC CACGCTGTTT
    GCCCTGGTCC GTACAGCTCT GAGGATCAAG ACGGAAGGGA ACCTGGAACA GGCCAATGAG
    GAGCTGCGGG CCATCATCAA GAAGATCTGG AAACGGACCA GCATGAAGCT GCTGGACCAA
    GTGGTGCCGC CCGCAGGAGA TGACGAGGTC ACGGTGGGCA AGTTTTACGC TACTTTCCTG
    ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC
    TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT GGCCTGCGCA CCCTGCACGA TCTGGGGCCC
    GAGATCCGCC GGGCCATCTC TGGGGACCTG ACCGCCGAGG AGGAGCTGGA CAAGGCCATG
    AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT GACATCTTCA GGAGGGCCGG CGGCCTGTTC
    GGCAACCACG TGGGCTACTA CCCGAGCGAC GGCCGGGGCG CCTTCCCCCA GACCTTCACC
    ACCCAGCGCC CCCTGCACAT CAGCAAGGCA GGCAACAGCC AGGGGGACAC CGAGTCCCCC
    TCCCACGAGA AACTGGTGGA CTCCACCTTC ACCCCCAGCA GCTACTCATC CACGGGCTCC
    AACGCCAACA TCAACAACGC CAACAACACC GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC
    CACTCCGGTG CTGTCAGCAC CTTGGAGGCC CACGGGCCCC CCTTGTCCCC TGCCATCCGG
    GTGCAGGAGG CCGCGTGGAG GCACAGCTCC CAGAGGTGCC CCTCCCGGGA CAGCCCGATA
    GCCATGCTGT GTCAGGAAGA GGCCTCTGGG GACGAGACCT GTGCCGTGCA CGTGGACGAG
    AACGCAGAGC ACAGCAGCGA GCCCAGCTTG GTCTCCACGG AGATGCTCTC CTACCAGGAT
    GACGAGAATC GGCAGCTGAC TCCCCCCGAG GAGGACAGGA GGGACATCCG TCTGTCCCCA
    AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA GGTCGAAGGG CCTCCTTCCA CCTTGAATGT
    CTGAAGCGAC AGAAGAATCC TGGGGGAGAC GTCTCTCAGA ACACAGTCCT GCCTTTGCAC
    CTGGTTCACC ATCAGGCGCT GGCAGTGGCG GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT
    CCCCCTGGGA CCCTCCCCCC ACCACGCCTC ACACCCCCCG CCACACCTGG CCCAGCCTGG
    CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG GAAGGGGCCG AGTCCAGCGA CAAGCTCACC
    AGCAGCTTCC CATCCATCCA CTGCGACCCC CACATCGGGG AGCCCACGCC CTGCGGGGTT
    GTCGGCACCC CCCGGAGGGC CCGGCCCGTG TCACTCACTG TGCCCAGCCC GGCAGGGCCC
    CAGGGCCGTC CGTTCCATGG CAGTGCCAGC AGCCTGGTGG AGGCGGTCTT GATCTCGGAA
    GGATTGGGGC AGTTTGCTCA GGACCCCAGG TTCCTCGAGG CCACCACGCA GGAGCTGGCC
    GATGCCTGCG ACATGACCAT TGAGGAGATG GAGAGCGCGG CTGACGACAT CCTCAGCGGG
    GGCGCCGGGC AGAGCCCCAA CGGCACCCTG CTCCCGTGTG CAAACTGCAG GGACCCGGGG
    CCGGACCGAG CGGGGGGTGT GGAGGACGCA GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG
    GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC TTCGCCAGCG GCCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.