CACNA1C cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA1C
Entrez Gene ID	408015
Full Name	calcium voltage-gated channel subunit alpha1 C
Synonyms	CAV1.2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015463565.1	XP_015319051.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
XM_015463570.1	XP_015319056.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
XM_015463572.1	XP_015319058.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
XM_015463575.1	XP_015319061.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
XM_015463578.1	XP_015319064.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
XM_005207303.3	XP_005207360.2	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
XM_010805693.2	XP_010803995.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
XM_010805694.2	XP_010803996.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
XM_015471361.1	XP_015326847.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
XM_005207302.3	XP_005207359.2	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
XM_015471365.1	XP_015326851.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
XM_015471366.1	XP_015326852.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
XM_015471368.1	XP_015326854.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
XM_010805697.2	XP_010803999.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
XM_024992755.1	XP_024848523.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
XM_024992764.1	XP_024848532.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
XM_015471362.1	XP_015326848.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
XM_015471367.1	XP_015326853.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
XM_015471370.1	XP_015326856.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
XM_015463560.1	XP_015319046.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
XM_015463561.1	XP_015319047.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
XM_015463573.1	XP_015319059.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
XM_015463574.1	XP_015319060.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
XM_024992747.1	XP_024848515.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
XM_024992757.1	XP_024848525.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
XM_024992758.1	XP_024848526.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
XM_024992761.1	XP_024848529.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
XM_024992763.1	XP_024848531.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
XM_015463577.1	XP_015319063.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X21
XM_010805695.2	XP_010803997.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
XM_010805696.2	XP_010803998.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
XM_010805698.2	XP_010804000.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
XM_015463564.1	XP_015319050.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
XM_015463568.1	XP_015319054.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
XM_015463569.1	XP_015319055.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
XM_024992746.1	XP_024848514.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
XM_024992750.1	XP_024848518.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
XM_024992752.1	XP_024848520.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
XM_024992754.1	XP_024848522.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
XM_015471364.1	XP_015326850.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
XM_015471369.1	XP_015326855.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
XM_015463567.1	XP_015319053.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
XM_015463571.1	XP_015319057.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
XM_015463558.1	XP_015319044.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
XM_015463559.1	XP_015319045.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
XM_015463566.1	XP_015319052.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
XM_024992748.1	XP_024848516.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
XM_024992756.1	XP_024848524.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
XM_024992760.1	XP_024848528.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
XM_015471363.1	XP_015326849.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
XM_015471371.1	XP_015326857.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
XM_015471372.1	XP_015326858.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
XM_015463562.1	XP_015319048.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
XM_015463563.1	XP_015319049.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
XM_015463576.1	XP_015319062.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
XM_024992749.1	XP_024848517.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
XM_024992753.1	XP_024848521.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
XM_024992759.1	XP_024848527.1	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13

Pathways from BioSystems

Homologies

Bos taurus (cattle)	CACNA1C	XP_005207410.1
Pan troglodytes (chimpanzee)	LOC466915	XP_522315.3

Gene Ontology

The following CACNA1C gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA1C cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa89989	XM_015471361.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89989	XM_015463561.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89990	XM_015463563.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89990	XM_015471362.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89991	XM_015471363.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89991	XM_015463565.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89992	XM_015471364.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89992	XM_015463566.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89993	XM_015471365.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89993	XM_015463567.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89994	XM_015471366.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89994	XM_015463568.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89995	XM_015471367.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89995	XM_015463569.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89996	XM_015463570.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89996	XM_015471368.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89997	XM_015463573.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89997	XM_015471369.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89998	XM_015463574.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89998	XM_015471370.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89999	XM_015463575.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa89999	XM_015471371.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa90000	XM_015463577.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa90001	XM_015471372.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa90001	XM_015463578.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa45786	XM_010805693.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45786	XM_015463559.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45787	XM_005207303.3 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45787	XM_015463558.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45788	XM_015463560.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45788	XM_010805694.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45789	XM_015463562.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45789	XM_005207302.3 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45790	XM_010805695.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45790	XM_015463564.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45791	XM_010805696.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45791	XM_015463571.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45792	XM_010805697.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45792	XM_015463572.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45793	XM_015463576.1 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa45793	XM_010805698.2 Latest version!	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa234933	XM_024992746.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234934	XM_024992747.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234935	XM_024992748.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234936	XM_024992749.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234937	XM_024992750.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234938	XM_024992752.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234939	XM_024992753.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234940	XM_024992754.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234941	XM_024992755.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234942	XM_024992756.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234943	XM_024992757.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234944	XM_024992758.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234945	XM_024992759.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234946	XM_024992760.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234947	XM_024992761.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234948	XM_024992763.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OBa234949	XM_024992764.1 Latest version!	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA1C ( XM_015471361.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326847.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

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CloneID	OBa89989
Clone ID Related Accession (Same CDS sequence)	XM_015471361.1 , XM_015463561.1
Accession Version	XM_015471361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326847.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471361.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471361.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463561.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319047.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

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CloneID	OBa89989
Clone ID Related Accession (Same CDS sequence)	XM_015471361.1 , XM_015463561.1
Accession Version	XM_015463561.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319047.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463561.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463561.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471362.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326848.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

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CloneID	OBa89990
Clone ID Related Accession (Same CDS sequence)	XM_015471362.1 , XM_015463563.1
Accession Version	XM_015471362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326848.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471362.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471362.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463563.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319049.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

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CloneID	OBa89990
Clone ID Related Accession (Same CDS sequence)	XM_015471362.1 , XM_015463563.1
Accession Version	XM_015463563.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319049.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463563.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463563.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463565.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319051.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

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CloneID	OBa89991
Clone ID Related Accession (Same CDS sequence)	XM_015463565.1 , XM_015471363.1
Accession Version	XM_015463565.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319051.1
CDS	1919..7129
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463565.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463565.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471363.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326849.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

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CloneID	OBa89991
Clone ID Related Accession (Same CDS sequence)	XM_015463565.1 , XM_015471363.1
Accession Version	XM_015471363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326849.1
CDS	1919..7129
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471363.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471363.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471364.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326850.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

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CloneID	OBa89992
Clone ID Related Accession (Same CDS sequence)	XM_015471364.1 , XM_015463566.1
Accession Version	XM_015471364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326850.1
CDS	1917..7121
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471364.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471364.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463566.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319052.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

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CloneID	OBa89992
Clone ID Related Accession (Same CDS sequence)	XM_015471364.1 , XM_015463566.1
Accession Version	XM_015463566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319052.1
CDS	1917..7121
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463566.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463566.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471365.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326851.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa89993
Clone ID Related Accession (Same CDS sequence)	XM_015471365.1 , XM_015463567.1
Accession Version	XM_015471365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326851.1
CDS	1918..7122
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471365.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471365.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463567.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319053.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

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CloneID	OBa89993
Clone ID Related Accession (Same CDS sequence)	XM_015471365.1 , XM_015463567.1
Accession Version	XM_015463567.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319053.1
CDS	1918..7122
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463567.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463567.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGTG 
AGGGGCCCTT GCCCCTGGGG ACCTGGCACC TCCTCCCACC TCTTCCAGGT TCCACGCTCC 
CACGGGTCCC TTCCGGAAAT TCCGAGCCTG CAGGGGGACC CTCCCCTCCC GCCCTGCCTC 
CTGCACTCAC TGCCCTCTGT AGTTCTGGAC TCTCAGCCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471366.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326852.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

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CloneID	OBa89994
Clone ID Related Accession (Same CDS sequence)	XM_015471366.1 , XM_015463568.1
Accession Version	XM_015471366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC 
ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326852.1
CDS	1918..7119
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471366.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471366.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC 
ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463568.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319054.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

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CloneID	OBa89994
Clone ID Related Accession (Same CDS sequence)	XM_015471366.1 , XM_015463568.1
Accession Version	XM_015463568.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC 
ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319054.1
CDS	1918..7119
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463568.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463568.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGCACT ATTTCTGTGA TGCATGGAAC ACATTTGACG CCTTGATTGT TGTGGGTAGC 
ATTGTTGATA TAGCCATCAC CGAGGTAAAC CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471367.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326853.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

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CloneID	OBa89995
Clone ID Related Accession (Same CDS sequence)	XM_015471367.1 , XM_015463569.1
Accession Version	XM_015471367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC 
GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326853.1
CDS	1918..7119
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471367.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471367.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC 
GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463569.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319055.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

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CloneID	OBa89995
Clone ID Related Accession (Same CDS sequence)	XM_015471367.1 , XM_015463569.1
Accession Version	XM_015463569.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC 
GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319055.1
CDS	1918..7119
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463569.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463569.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGCAGAAGA CGACCCCTCG 
CCCTGTGCCC TGGAGACAGG CCACGGGCGG CAGTGCCAGA ATGGCACGGT GTGCAAACCA 
GGCTGGGATG GACCCAAGCA CGGCATCACC AATTTCGACA ACTTCGCCTT CGCCATGCTG 
ACTGTGTTCC AGTGCATCAC CATGGAGGGC TGGACCGACG TCCTCTACTG GGTCAATGAT 
GCCGTGGGAA GGGACTGGCC CTGGATCTAT TTTGTCACAT TAATCATCAT AGGTTCATTC 
TTTGTTCTTA ACTTGGTTCT CGGTGTCCTT AGCGGAGAGT TCTCCAAAGA GCGAGAGAAG 
GCCAAGGCCC GGGGAGACTT CCAGAAGCTG CGAGAGAAGC AGCAGCTGGA GGAGGACCTG 
AAGGGCTACC TGGACTGGAT CACGCAGGCC GAGGACATCG ACCCCGAGAA TGAGGACGAG 
GGCCTGGACG AGGAGAAACC CCGAAACATG AGCATGCCCA CCAGTGAGAC CGAGTCTGTC 
AACACCGAAA ACGTGGCTGG AGGGGACATC GAGGGAGAGA GCTGCGGGGC CAGGCTGGCC 
CACCGGATCT CCAAGTCAAA GTTCAGCCGC TACTGGCGCC GGTGGAATCG GTTCTGCAGG 
AGGAAGTGCC GCGCGGCCGT GAAGTCCAGC ATCTTCTACT GGCTGGTCAT CTTCCTGGTT 
TTCCTCAACA CACTCACCAT CGCCTCCGAG CACTACAACC AGCCCCACTG GCTCACGGAA 
GTCCAAGACA CGGCCAACAA GGCGCTGCTG GCCCTGTTCA CGGCGGAGAT GCTGCTCAAG 
ATGTACAGCC TGGGCCTGCA GGCCTACTTC GTGTCGCTCT TCAACCGCTT CGACTGCTTC 
ATCGTGTGCG GGGGCATCCT GGAGACCATC CTGGTGGAGA CCAAGATCAT GTCGCCGTTG 
GGCATCTCCG TGCTGCGTTG CGTGCGCCTC CTTCGGATAT TTAAGATCAC AAGGTACTGG 
AACTCCCTGA GCAACCTGGT GGCATCCCTG CTGAACTCAG TGCGCTCCAT TGCCTCGCTG 
CTGCTGCTGC TCTTCCTCTT CATCATCATC TTCTCCCTCC TGGGGATGCA GCTCTTTGGG 
GGGAAGTTCA ACTTTGATGA GATGCAGACC CGGAGGAGCA CCTTTGACAA CTTCCCGCAG 
TCCCTCCTCA CCGTGTTTCA GATCCTGACC GGGGAGGACT GGAATTCGGT GATGTATGAT 
GGGATCATGG CTTATGGCGG CCCCTCTTTT CCAGGGATGT TAGTCTGTAT TTACTTCATC 
ATCCTCTTCA TCTGTGGAAA TTATATCCTA CTGAACGTGT TCTTGGCCAT TGCTGTGGAC 
AACCTGGCTG ACGCGGAGAG CCTCACATCT GCCCAGAAGG AGGAGGAAGA GGAGAAGGAG 
AGGAAGAAGC TAGCCAGGAC CGCCAGCCCA GAGAAGAAGC AAGAGGTGGT AGAGAAGCCG 
GCCGTGGAGG AGACCAAGGA GGAGAAAATC GAGCTGAAAT CCATCACGGC AGATGGAGAG 
TCCCCACCCG CCACCAAGAT CAACATGGAT GACCTCCAGC CCAACGAGAA TGAGGACAAG 
AGCTCCTACC CCAACCAGGA AACCACAGGA GAAGAGGACG AGGAGGAGCC CGAGATGCCC 
GTGGGCCCCC GCCCGCGCCC CCTCTCTGAG CTGCACCTGA AGGAGAAGGC AGTGCCCATG 
CCGGAAGCCA GCGCCTTCTT CATCTTCAGC CCCAGCAACA GGTTCCGCCT CCAGTGCCAT 
CGCATCGTCA ATGACTCCAT CTTCACCAAC CTGATCCTCT TCTTCATCCT GCTCAGCAGC 
ATCTCCCTGG CCGCCGAGGA CCCCGTGCAG CACACCTCCT TCAGGAACCA CATTCTGTTT 
TATTTTGACA TTGTTTTCAC TACCATTTTC ACCATTGAGA TTGCCCTGAA GATGACGGCC 
TACGGGGCCT TCCTGCACAA GGGCTCCTTC TGCCGCAACT ACTTCAACAT CCTGGACCTG 
CTGGTGGTCA GTGTGTCCCT CATCTCCTTC GGCATCCAGT CCAGTGCCAT CAACGTGGTG 
AAGATCCTGC GAGTCCTGAG GGTGCTCCGG CCACTGAGGG CCATCAACAG GGCCAAGGGG 
CTGAAGCACG TCGTGCAGTG CGTGTTCGTG GCCATCCGCA CCATCGGGAA CATTGTGATC 
GTCACCACGC TGCTCCAGTT CATGTTCGCC TGCATTGGGG TCCAGCTCTT CAAGGGAAAG 
CTCTACACCT GTTCGGACAG TTCCAAACAG ACAGAGGCTG AATGCAAGGG GAACTACATA 
ACATACAAGG ATGGGGAGGT GGACCACCCC ATCATCCAGC CCCGCAGCTG GGAGAACAGC 
AAGTTTGACT TTGACAATGT CCTGGCAGCC ATGATGGCCC TGTTCACCGT CTCCACCTTC 
GAGGGATGGC CAGAGTTGCT GTACCGCTCC ATCGACTCTC ACACGGAGGA CAAGGGTCCC 
ATATACAACT ACCGCGTGGA GATCTCCGTC TTCTTCATCA TCTACATCAT CATCATTGCC 
TTCTTCATGA TGAACATCTT CGTGGGTTTC GTCATCGTCA CCTTCCAGGA GCAGGGGGAG 
CAGGAATACA AGAACTGCGA GCTGGACAAG AACCAGCGGC AGTGTGTGGA GTATGCCCTC 
AAGGCCCGGC CCCTGCGGAG GTACATCCCG AAAAACCAGC ACCAGTACAA GGTGTGGTAC 
GTGGTCAACT CCACCTACTT CGAGTACCTG ATGTTCGTTC TCATTCTGCT CAACACCATC 
TGCCTGGCCA TGCAGCACTA CGGTCAGAGC TGTCTGTTCA AAATCGCCAT GAACATCCTC 
AACATGCTCT TCACCGGCCT CTTCACAGTG GAGATGGTCC TGAAGCTCAT CGCCTTCAAG 
CCCAAGGGCT ACTTTAGTGA CCCCTGGAAT GTCTTTGACT TCCTGATCGT AATTGGCAGC 
GTAATTGACG TCATTCTCAG TGAGACGAAT CCAGCTGAAC ATACCCAATG CTCTCCCTTT 
ATGAACGCGG AAGAGAACTC TCGCATCTCC ATCACCTTCT TCCGCCTCTT CCGGGTCATG 
CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG GGCATCCGGA CACTGCTGTG GACCTTCATC 
AAGTCCTTCC AGGCCCTACC CTATGTGGCC CTCCTGATCG TGATGCTGTT TTTCATCTAC 
GCCGTGATCG GGATGCAGGT GTTTGGCAAA ATTGCCCTGA ATGACACGAC AGAGATCAAC 
AGGAACAACA ACTTCCAGAC CTTCCCCCAG GCCGTGCTGC TCCTCTTCAG GTGCGCCACT 
GGGGAGGCCT GGCAGGACAT CATGCTGGCC TGCATGCCTG GCAAGAAGTG CGCCCCCGAG 
TCAGAGCCGG GCAACAGCAC AGAGGGGGAG ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC 
TACTTCATCA GCTTCTACAT GCTCTGCGCC TTCCTGATCA TCAACCTCTT CGTAGCCGTC 
ATCATGGACA ACTTTGACTA CCTGACGAGG GACTGGTCCA TCCTGGGTCC CCACCACCTG 
GACGAATTTA AGAGAATTTG GGCAGAGTAT GACCCTGAAG CCAAGGGACG CATCAAACAC 
CTGGATGTGG TGACCCTGCT CCGACGGATT CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC 
CCTCACCGCG TGGCTTGCAA ACGCCTGGTC TCCATGAACA TGCCCCTGAA CAGCGACGGG 
ACTGTCATGT TCAATGCCAC GCTGTTTGCC CTGGTCCGTA CAGCTCTGAG GATCAAGACG 
GAAGGGAACC TGGAACAGGC CAATGAGGAG CTGCGGGCCA TCATCAAGAA GATCTGGAAA 
CGGACCAGCA TGAAGCTGCT GGACCAAGTG GTGCCGCCCG CAGGAGATGA CGAGGTCACG 
GTGGGCAAGT TTTACGCTAC TTTCCTGATC CAGGAGTATT TCCGGAAGTT CAAGAAGCGC 
AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC CAGAGGAACG CCCTGTCCCT GCAGGTGAGG 
GGCCCTTGCC CCTGGGGACC TGGCACCTCC TCCCACCTCT TCCAGGTTCC ACGCTCCCAC 
GGGTCCCTTC CGGAAATTCC GAGCCTGCAG GGGGACCCTC CCCTCCCGCC CTGCCTCCTG 
CACTCACTGC CCTCTGTAGT TCTGGACTCT CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463570.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319056.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

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CloneID	OBa89996
Clone ID Related Accession (Same CDS sequence)	XM_015463570.1 , XM_015471368.1
Accession Version	XM_015463570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC 
CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG 
TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC 
TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319056.1
CDS	1918..7116
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463570.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463570.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC 
CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG 
TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC 
TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471368.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326854.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

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CloneID	OBa89996
Clone ID Related Accession (Same CDS sequence)	XM_015463570.1 , XM_015471368.1
Accession Version	XM_015471368.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5199bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC 
CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG 
TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC 
TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326854.1
CDS	1918..7116
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471368.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471368.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGTGAGGGGC 
CCTTGCCCCT GGGGACCTGG CACCTCCTCC CACCTCTTCC AGGTTCCACG CTCCCACGGG 
TCCCTTCCGG AAATTCCGAG CCTGCAGGGG GACCCTCCCC TCCCGCCCTG CCTCCTGCAC 
TCACTGCCCT CTGTAGTTCT GGACTCTCAG CCCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463573.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319059.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OBa89997
Clone ID Related Accession (Same CDS sequence)	XM_015463573.1 , XM_015471369.1
Accession Version	XM_015463573.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319059.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463573.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463573.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471369.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326855.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

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CloneID	OBa89997
Clone ID Related Accession (Same CDS sequence)	XM_015463573.1 , XM_015471369.1
Accession Version	XM_015471369.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326855.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471369.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471369.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471370.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326856.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

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CloneID	OBa89998
Clone ID Related Accession (Same CDS sequence)	XM_015471370.1 , XM_015463574.1
Accession Version	XM_015471370.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326856.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471370.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471370.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463574.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319060.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

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CloneID	OBa89998
Clone ID Related Accession (Same CDS sequence)	XM_015471370.1 , XM_015463574.1
Accession Version	XM_015463574.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319060.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463574.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463574.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463575.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319061.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

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CloneID	OBa89999
Clone ID Related Accession (Same CDS sequence)	XM_015463575.1 , XM_015471371.1
Accession Version	XM_015463575.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319061.1
CDS	1919..7096
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463575.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463575.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471371.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326857.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

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CloneID	OBa89999
Clone ID Related Accession (Same CDS sequence)	XM_015463575.1 , XM_015471371.1
Accession Version	XM_015471371.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326857.1
CDS	1919..7096
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471371.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471371.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463577.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319063.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X21, mRNA.

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CloneID	OBa90000
Clone ID Related Accession (Same CDS sequence)	XM_015463577.1
Accession Version	XM_015463577.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACGCCA ACGCGGCCGC GGGGCTGGCA CCTGAGCACA TCCCCACACC GGGGGCCGCC 
CTGTCCTGGC AGGCCGCCAT CGACGCGGCC CGGCAGGCCA AGCTGATGGG CAGTGCTGGC 
AACGCAACCA TCTCCACGGT CAGCTCCACG CAGCGGAAAC GGCAGCAGTA TGGGAAGCCC 
AAGAAGCAGG GCAGCACGAC AGCCACCCGC CCGCCCCGCG CCCTGCTCTG CCTGACCCTA 
AAGAACCCCA TCCGGAGGGC CTGCATCAGC ATCGTCGAGT GGAAGCCATT TGAAATCATT 
ATTTTACTGA CTATTTTTGC CAATTGTGTG GCCTTAGCGA TCTATATTCC CTTCCCAGAG 
GACGACTCCA ACGCCACGAA TTCCAACCTG GAGCGGGTGG AGTATCTCTT CCTCATCATT 
TTTACTGTGG AAGCATTTTT AAAAGTCATA GCCTACGGAC TCCTCTTTCA CCCCAACGCT 
TACCTCCGCA ATGGCTGGAA CTTACTAGAC TTTATCATCG TGGTTGTCGG GCTTTTCAGC 
GCGATCTTAG AGCAAGCGAC CAAAGCCGAT GGGGCCAACG CCCTGGGAGG GAAGGGTGCT 
GGATTCGACG TAAAGGCGCT GAGGGCGTTC CGTGTGCTAC GGCCCCTGCG GTTGGTGTCC 
GGGGTCCCGA GTCTCCAGGT GGTCTTGAAT TCCATCATCA AGGCCATGGT GCCCCTGCTG 
CACATCGCCC TGCTCGTGCT GTTCGTCATC ATCATCTATG CCATCATCGG CCTGGAGCTC 
TTCATGGGCA AGATGCATAA GACCTGCTAC AACCAGGAGG GCATTGCAGA TGTCCCAGCA 
GAAGACGACC CCTCGCCCTG TGCCCTGGAG ACAGGCCACG GGCGGCAGTG CCAGAATGGC 
ACGGTGTGCA AACCAGGCTG GGATGGACCC AAGCACGGCA TCACCAATTT CGACAACTTC 
GCCTTCGCCA TGCTGACTGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACGTCCTC 
TACTGGGTCA ATGATGCCGT GGGAAGGGAC TGGCCCTGGA TCTATTTTGT CACATTAATC 
ATCATAGGTT CATTCTTTGT TCTTAACTTG GTTCTCGGTG TCCTTAGCGG AGAGTTCTCC 
AAAGAGCGAG AGAAGGCCAA GGCCCGGGGA GACTTCCAGA AGCTGCGAGA GAAGCAGCAG 
CTGGAGGAGG ACCTGAAGGG CTACCTGGAC TGGATCACGC AGGCCGAGGA CATCGACCCC 
GAGAATGAGG ACGAGGGCCT GGACGAGGAG AAACCCCGAA ACATGAGCAT GCCCACCAGT 
GAGACCGAGT CTGTCAACAC CGAAAACGTG GCTGGAGGGG ACATCGAGGG AGAGAGCTGC 
GGGGCCAGGC TGGCCCACCG GATCTCCAAG TCAAAGTTCA GCCGCTACTG GCGCCGGTGG 
AATCGGTTCT GCAGGAGGAA GTGCCGCGCG GCCGTGAAGT CCAGCATCTT CTACTGGCTG 
GTCATCTTCC TGGTTTTCCT CAACACACTC ACCATCGCCT CCGAGCACTA CAACCAGCCC 
CACTGGCTCA CGGAAGTCCA AGACACGGCC AACAAGGCGC TGCTGGCCCT GTTCACGGCG 
GAGATGCTGC TCAAGATGTA CAGCCTGGGC CTGCAGGCCT ACTTCGTGTC GCTCTTCAAC 
CGCTTCGACT GCTTCATCGT GTGCGGGGGC ATCCTGGAGA CCATCCTGGT GGAGACCAAG 
ATCATGTCGC CGTTGGGCAT CTCCGTGCTG CGTTGCGTGC GCCTCCTTCG GATATTTAAG 
ATCACAAGGT ACTGGAACTC CCTGAGCAAC CTGGTGGCAT CCCTGCTGAA CTCAGTGCGC 
TCCATTGCCT CGCTGCTGCT GCTGCTCTTC CTCTTCATCA TCATCTTCTC CCTCCTGGGG 
ATGCAGCTCT TTGGGGGGAA GTTCAACTTT GATGAGATGC AGACCCGGAG GAGCACCTTT 
GACAACTTCC CGCAGTCCCT CCTCACCGTG TTTCAGATCC TGACCGGGGA GGACTGGAAT 
TCGGTGATGT ATGATGGGAT CATGGCTTAT GGCGGCCCCT CTTTTCCAGG GATGTTAGTC 
TGTATTTACT TCATCATCCT CTTCATCTGT GGAAATTATA TCCTACTGAA CGTGTTCTTG 
GCCATTGCTG TGGACAACCT GGCTGACGCG GAGAGCCTCA CATCTGCCCA GAAGGAGGAG 
GAAGAGGAGA AGGAGAGGAA GAAGCTAGCC AGGACCGCCA GCCCAGAGAA GAAGCAAGAG 
GTGGTAGAGA AGCCGGCCGT GGAGGAGACC AAGGAGGAGA AAATCGAGCT GAAATCCATC 
ACGGCAGATG GAGAGTCCCC ACCCGCCACC AAGATCAACA TGGATGACCT CCAGCCCAAC 
GAGAATGAGG ACAAGAGCTC CTACCCCAAC CAGGAAACCA CAGGAGAAGA GGACGAGGAG 
GAGCCCGAGA TGCCCGTGGG CCCCCGCCCG CGCCCCCTCT CTGAGCTGCA CCTGAAGGAG 
AAGGCAGTGC CCATGCCGGA AGCCAGCGCC TTCTTCATCT TCAGCCCCAG CAACAGGTTC 
CGCCTCCAGT GCCATCGCAT CGTCAATGAC TCCATCTTCA CCAACCTGAT CCTCTTCTTC 
ATCCTGCTCA GCAGCATCTC CCTGGCCGCC GAGGACCCCG TGCAGCACAC CTCCTTCAGG 
AACCACATTC TGTTTTATTT TGACATTGTT TTCACTACCA TTTTCACCAT TGAGATTGCC 
CTGAAGATGA CGGCCTACGG GGCCTTCCTG CACAAGGGCT CCTTCTGCCG CAACTACTTC 
AACATCCTGG ACCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTCGGCAT CCAGTCCAGT 
GCCATCAACG TGGTGAAGAT CCTGCGAGTC CTGAGGGTGC TCCGGCCACT GAGGGCCATC 
AACAGGGCCA AGGGGCTGAA GCACGTCGTG CAGTGCGTGT TCGTGGCCAT CCGCACCATC 
GGGAACATTG TGATCGTCAC CACGCTGCTC CAGTTCATGT TCGCCTGCAT TGGGGTCCAG 
CTCTTCAAGG GAAAGCTCTA CACCTGTTCG GACAGTTCCA AACAGACAGA GGCTGAATGC 
AAGGGGAACT ACATAACATA CAAGGATGGG GAGGTGGACC ACCCCATCAT CCAGCCCCGC 
AGCTGGGAGA ACAGCAAGTT TGACTTTGAC AATGTCCTGG CAGCCATGAT GGCCCTGTTC 
ACCGTCTCCA CCTTCGAGGG ATGGCCAGAG TTGCTGTACC GCTCCATCGA CTCTCACACG 
GAGGACAAGG GTCCCATATA CAACTACCGC GTGGAGATCT CCGTCTTCTT CATCATCTAC 
ATCATCATCA TTGCCTTCTT CATGATGAAC ATCTTCGTGG GTTTCGTCAT CGTCACCTTC 
CAGGAGCAGG GGGAGCAGGA ATACAAGAAC TGCGAGCTGG ACAAGAACCA GCGGCAGTGT 
GTGGAGTATG CCCTCAAGGC CCGGCCCCTG CGGAGGTACA TCCCGAAAAA CCAGCACCAG 
TACAAGGTGT GGTACGTGGT CAACTCCACC TACTTCGAGT ACCTGATGTT CGTTCTCATT 
CTGCTCAACA CCATCTGCCT GGCCATGCAG CACTACGGTC AGAGCTGTCT GTTCAAAATC 
GCCATGAACA TCCTCAACAT GCTCTTCACC GGCCTCTTCA CAGTGGAGAT GGTCCTGAAG 
CTCATCGCCT TCAAGCCCAA GCACTATTTC TGTGATGCAT GGAACACATT TGACGCCTTG 
ATTGTTGTGG GTAGCATTGT TGATATAGCC ATCACCGAGG TAAACCCAGC TGAACATACC 
CAATGCTCTC CCTTTATGAA CGCGGAAGAG AACTCTCGCA TCTCCATCAC CTTCTTCCGC 
CTCTTCCGGG TCATGCGTCT GGTCAAGCTG CTGAGCCGCG GGGAGGGCAT CCGGACACTG 
CTGTGGACCT TCATCAAGTC CTTCCAGGCC CTACCCTATG TGGCCCTCCT GATCGTGATG 
CTGTTTTTCA TCTACGCCGT GATCGGGATG CAGGTGTTTG GCAAAATTGC CCTGAATGAC 
ACGACAGAGA TCAACAGGAA CAACAACTTC CAGACCTTCC CCCAGGCCGT GCTGCTCCTC 
TTCAGGTGCG CCACTGGGGA GGCCTGGCAG GACATCATGC TGGCCTGCAT GCCTGGCAAG 
AAGTGCGCCC CCGAGTCAGA GCCGGGCAAC AGCACAGAGG GGGAGACGCC CTGTGGCAGC 
AGCTTCGCCG TCTTCTACTT CATCAGCTTC TACATGCTCT GCGCCTTCCT GATCATCAAC 
CTCTTCGTAG CCGTCATCAT GGACAACTTT GACTACCTGA CGAGGGACTG GTCCATCCTG 
GGTCCCCACC ACCTGGACGA ATTTAAGAGA ATTTGGGCAG AGTATGACCC TGAAGCCAAG 
GGACGCATCA AACACCTGGA TGTGGTGACC CTGCTCCGAC GGATTCAGCC CCCCCTGGGT 
TTTGGGAAGC TGTGCCCTCA CCGCGTGGCT TGCAAACGCC TGGTCTCCAT GAACATGCCC 
CTGAACAGCG ACGGGACTGT CATGTTCAAT GCCACGCTGT TTGCCCTGGT CCGTACAGCT 
CTGAGGATCA AGACGGAAGG GAACCTGGAA CAGGCCAATG AGGAGCTGCG GGCCATCATC 
AAGAAGATCT GGAAACGGAC CAGCATGAAG CTGCTGGACC AAGTGGTGCC GCCCGCAGGA 
GATGACGAGG TCACGGTGGG CAAGTTTTAC GCTACTTTCC TGATCCAGGA GTATTTCCGG 
AAGTTCAAGA AGCGCAAAGA GCAAGGCCTG GTGGGCAAGC CCTCCCAGAG GAACGCCCTG 
TCCCTGCAGG TGAGGGGCCC TTGCCCCTGG GGACCTGGCA CCTCCTCCCA CCTCTTCCAG 
GTTCCACGCT CCCACGGGTC CCTTCCGGAA ATTCCGAGCC TGCAGGGGGA CCCTCCCCTC 
CCGCCCTGCC TCCTGCACTC ACTGCCCTCT GTAGTTCTGG ACTCTCAGCC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319063.1
CDS	424..5460
Translation	MNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463577.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463577.1

ORF Insert Sequence:

ATGAACGCCA ACGCGGCCGC GGGGCTGGCA CCTGAGCACA TCCCCACACC GGGGGCCGCC 
CTGTCCTGGC AGGCCGCCAT CGACGCGGCC CGGCAGGCCA AGCTGATGGG CAGTGCTGGC 
AACGCAACCA TCTCCACGGT CAGCTCCACG CAGCGGAAAC GGCAGCAGTA TGGGAAGCCC 
AAGAAGCAGG GCAGCACGAC AGCCACCCGC CCGCCCCGCG CCCTGCTCTG CCTGACCCTA 
AAGAACCCCA TCCGGAGGGC CTGCATCAGC ATCGTCGAGT GGAAGCCATT TGAAATCATT 
ATTTTACTGA CTATTTTTGC CAATTGTGTG GCCTTAGCGA TCTATATTCC CTTCCCAGAG 
GACGACTCCA ACGCCACGAA TTCCAACCTG GAGCGGGTGG AGTATCTCTT CCTCATCATT 
TTTACTGTGG AAGCATTTTT AAAAGTCATA GCCTACGGAC TCCTCTTTCA CCCCAACGCT 
TACCTCCGCA ATGGCTGGAA CTTACTAGAC TTTATCATCG TGGTTGTCGG GCTTTTCAGC 
GCGATCTTAG AGCAAGCGAC CAAAGCCGAT GGGGCCAACG CCCTGGGAGG GAAGGGTGCT 
GGATTCGACG TAAAGGCGCT GAGGGCGTTC CGTGTGCTAC GGCCCCTGCG GTTGGTGTCC 
GGGGTCCCGA GTCTCCAGGT GGTCTTGAAT TCCATCATCA AGGCCATGGT GCCCCTGCTG 
CACATCGCCC TGCTCGTGCT GTTCGTCATC ATCATCTATG CCATCATCGG CCTGGAGCTC 
TTCATGGGCA AGATGCATAA GACCTGCTAC AACCAGGAGG GCATTGCAGA TGTCCCAGCA 
GAAGACGACC CCTCGCCCTG TGCCCTGGAG ACAGGCCACG GGCGGCAGTG CCAGAATGGC 
ACGGTGTGCA AACCAGGCTG GGATGGACCC AAGCACGGCA TCACCAATTT CGACAACTTC 
GCCTTCGCCA TGCTGACTGT GTTCCAGTGC ATCACCATGG AGGGCTGGAC CGACGTCCTC 
TACTGGGTCA ATGATGCCGT GGGAAGGGAC TGGCCCTGGA TCTATTTTGT CACATTAATC 
ATCATAGGTT CATTCTTTGT TCTTAACTTG GTTCTCGGTG TCCTTAGCGG AGAGTTCTCC 
AAAGAGCGAG AGAAGGCCAA GGCCCGGGGA GACTTCCAGA AGCTGCGAGA GAAGCAGCAG 
CTGGAGGAGG ACCTGAAGGG CTACCTGGAC TGGATCACGC AGGCCGAGGA CATCGACCCC 
GAGAATGAGG ACGAGGGCCT GGACGAGGAG AAACCCCGAA ACATGAGCAT GCCCACCAGT 
GAGACCGAGT CTGTCAACAC CGAAAACGTG GCTGGAGGGG ACATCGAGGG AGAGAGCTGC 
GGGGCCAGGC TGGCCCACCG GATCTCCAAG TCAAAGTTCA GCCGCTACTG GCGCCGGTGG 
AATCGGTTCT GCAGGAGGAA GTGCCGCGCG GCCGTGAAGT CCAGCATCTT CTACTGGCTG 
GTCATCTTCC TGGTTTTCCT CAACACACTC ACCATCGCCT CCGAGCACTA CAACCAGCCC 
CACTGGCTCA CGGAAGTCCA AGACACGGCC AACAAGGCGC TGCTGGCCCT GTTCACGGCG 
GAGATGCTGC TCAAGATGTA CAGCCTGGGC CTGCAGGCCT ACTTCGTGTC GCTCTTCAAC 
CGCTTCGACT GCTTCATCGT GTGCGGGGGC ATCCTGGAGA CCATCCTGGT GGAGACCAAG 
ATCATGTCGC CGTTGGGCAT CTCCGTGCTG CGTTGCGTGC GCCTCCTTCG GATATTTAAG 
ATCACAAGGT ACTGGAACTC CCTGAGCAAC CTGGTGGCAT CCCTGCTGAA CTCAGTGCGC 
TCCATTGCCT CGCTGCTGCT GCTGCTCTTC CTCTTCATCA TCATCTTCTC CCTCCTGGGG 
ATGCAGCTCT TTGGGGGGAA GTTCAACTTT GATGAGATGC AGACCCGGAG GAGCACCTTT 
GACAACTTCC CGCAGTCCCT CCTCACCGTG TTTCAGATCC TGACCGGGGA GGACTGGAAT 
TCGGTGATGT ATGATGGGAT CATGGCTTAT GGCGGCCCCT CTTTTCCAGG GATGTTAGTC 
TGTATTTACT TCATCATCCT CTTCATCTGT GGAAATTATA TCCTACTGAA CGTGTTCTTG 
GCCATTGCTG TGGACAACCT GGCTGACGCG GAGAGCCTCA CATCTGCCCA GAAGGAGGAG 
GAAGAGGAGA AGGAGAGGAA GAAGCTAGCC AGGACCGCCA GCCCAGAGAA GAAGCAAGAG 
GTGGTAGAGA AGCCGGCCGT GGAGGAGACC AAGGAGGAGA AAATCGAGCT GAAATCCATC 
ACGGCAGATG GAGAGTCCCC ACCCGCCACC AAGATCAACA TGGATGACCT CCAGCCCAAC 
GAGAATGAGG ACAAGAGCTC CTACCCCAAC CAGGAAACCA CAGGAGAAGA GGACGAGGAG 
GAGCCCGAGA TGCCCGTGGG CCCCCGCCCG CGCCCCCTCT CTGAGCTGCA CCTGAAGGAG 
AAGGCAGTGC CCATGCCGGA AGCCAGCGCC TTCTTCATCT TCAGCCCCAG CAACAGGTTC 
CGCCTCCAGT GCCATCGCAT CGTCAATGAC TCCATCTTCA CCAACCTGAT CCTCTTCTTC 
ATCCTGCTCA GCAGCATCTC CCTGGCCGCC GAGGACCCCG TGCAGCACAC CTCCTTCAGG 
AACCACATTC TGTTTTATTT TGACATTGTT TTCACTACCA TTTTCACCAT TGAGATTGCC 
CTGAAGATGA CGGCCTACGG GGCCTTCCTG CACAAGGGCT CCTTCTGCCG CAACTACTTC 
AACATCCTGG ACCTGCTGGT GGTCAGTGTG TCCCTCATCT CCTTCGGCAT CCAGTCCAGT 
GCCATCAACG TGGTGAAGAT CCTGCGAGTC CTGAGGGTGC TCCGGCCACT GAGGGCCATC 
AACAGGGCCA AGGGGCTGAA GCACGTCGTG CAGTGCGTGT TCGTGGCCAT CCGCACCATC 
GGGAACATTG TGATCGTCAC CACGCTGCTC CAGTTCATGT TCGCCTGCAT TGGGGTCCAG 
CTCTTCAAGG GAAAGCTCTA CACCTGTTCG GACAGTTCCA AACAGACAGA GGCTGAATGC 
AAGGGGAACT ACATAACATA CAAGGATGGG GAGGTGGACC ACCCCATCAT CCAGCCCCGC 
AGCTGGGAGA ACAGCAAGTT TGACTTTGAC AATGTCCTGG CAGCCATGAT GGCCCTGTTC 
ACCGTCTCCA CCTTCGAGGG ATGGCCAGAG TTGCTGTACC GCTCCATCGA CTCTCACACG 
GAGGACAAGG GTCCCATATA CAACTACCGC GTGGAGATCT CCGTCTTCTT CATCATCTAC 
ATCATCATCA TTGCCTTCTT CATGATGAAC ATCTTCGTGG GTTTCGTCAT CGTCACCTTC 
CAGGAGCAGG GGGAGCAGGA ATACAAGAAC TGCGAGCTGG ACAAGAACCA GCGGCAGTGT 
GTGGAGTATG CCCTCAAGGC CCGGCCCCTG CGGAGGTACA TCCCGAAAAA CCAGCACCAG 
TACAAGGTGT GGTACGTGGT CAACTCCACC TACTTCGAGT ACCTGATGTT CGTTCTCATT 
CTGCTCAACA CCATCTGCCT GGCCATGCAG CACTACGGTC AGAGCTGTCT GTTCAAAATC 
GCCATGAACA TCCTCAACAT GCTCTTCACC GGCCTCTTCA CAGTGGAGAT GGTCCTGAAG 
CTCATCGCCT TCAAGCCCAA GCACTATTTC TGTGATGCAT GGAACACATT TGACGCCTTG 
ATTGTTGTGG GTAGCATTGT TGATATAGCC ATCACCGAGG TAAACCCAGC TGAACATACC 
CAATGCTCTC CCTTTATGAA CGCGGAAGAG AACTCTCGCA TCTCCATCAC CTTCTTCCGC 
CTCTTCCGGG TCATGCGTCT GGTCAAGCTG CTGAGCCGCG GGGAGGGCAT CCGGACACTG 
CTGTGGACCT TCATCAAGTC CTTCCAGGCC CTACCCTATG TGGCCCTCCT GATCGTGATG 
CTGTTTTTCA TCTACGCCGT GATCGGGATG CAGGTGTTTG GCAAAATTGC CCTGAATGAC 
ACGACAGAGA TCAACAGGAA CAACAACTTC CAGACCTTCC CCCAGGCCGT GCTGCTCCTC 
TTCAGGTGCG CCACTGGGGA GGCCTGGCAG GACATCATGC TGGCCTGCAT GCCTGGCAAG 
AAGTGCGCCC CCGAGTCAGA GCCGGGCAAC AGCACAGAGG GGGAGACGCC CTGTGGCAGC 
AGCTTCGCCG TCTTCTACTT CATCAGCTTC TACATGCTCT GCGCCTTCCT GATCATCAAC 
CTCTTCGTAG CCGTCATCAT GGACAACTTT GACTACCTGA CGAGGGACTG GTCCATCCTG 
GGTCCCCACC ACCTGGACGA ATTTAAGAGA ATTTGGGCAG AGTATGACCC TGAAGCCAAG 
GGACGCATCA AACACCTGGA TGTGGTGACC CTGCTCCGAC GGATTCAGCC CCCCCTGGGT 
TTTGGGAAGC TGTGCCCTCA CCGCGTGGCT TGCAAACGCC TGGTCTCCAT GAACATGCCC 
CTGAACAGCG ACGGGACTGT CATGTTCAAT GCCACGCTGT TTGCCCTGGT CCGTACAGCT 
CTGAGGATCA AGACGGAAGG GAACCTGGAA CAGGCCAATG AGGAGCTGCG GGCCATCATC 
AAGAAGATCT GGAAACGGAC CAGCATGAAG CTGCTGGACC AAGTGGTGCC GCCCGCAGGA 
GATGACGAGG TCACGGTGGG CAAGTTTTAC GCTACTTTCC TGATCCAGGA GTATTTCCGG 
AAGTTCAAGA AGCGCAAAGA GCAAGGCCTG GTGGGCAAGC CCTCCCAGAG GAACGCCCTG 
TCCCTGCAGG TGAGGGGCCC TTGCCCCTGG GGACCTGGCA CCTCCTCCCA CCTCTTCCAG 
GTTCCACGCT CCCACGGGTC CCTTCCGGAA ATTCCGAGCC TGCAGGGGGA CCCTCCCCTC 
CCGCCCTGCC TCCTGCACTC ACTGCCCTCT GTAGTTCTGG ACTCTCAGCC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463578.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319064.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

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CloneID	OBa90001
Clone ID Related Accession (Same CDS sequence)	XM_015463578.1 , XM_015471372.1
Accession Version	XM_015463578.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319064.1
CDS	1919..7096
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463578.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463578.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015471372.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326858.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OBa90001
Clone ID Related Accession (Same CDS sequence)	XM_015463578.1 , XM_015471372.1
Accession Version	XM_015471372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326858.1
CDS	1919..7096
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015471372.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471372.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805693.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803995.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

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CloneID	OBa45786
Clone ID Related Accession (Same CDS sequence)	XM_010805693.2 , XM_015463559.1
Accession Version	XM_010805693.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803995.1
CDS	1917..7127
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805693.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805693.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463559.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319045.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

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CloneID	OBa45786
Clone ID Related Accession (Same CDS sequence)	XM_010805693.2 , XM_015463559.1
Accession Version	XM_015463559.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319045.1
CDS	1917..7127
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463559.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463559.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_005207303.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207360.2 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

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CloneID	OBa45787
Clone ID Related Accession (Same CDS sequence)	XM_005207303.3 , XM_015463558.1
Accession Version	XM_005207303.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207303.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207360.2
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_005207303.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207303.3

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463558.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319044.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

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CloneID	OBa45787
Clone ID Related Accession (Same CDS sequence)	XM_005207303.3 , XM_015463558.1
Accession Version	XM_015463558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319044.1
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463558.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463558.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805694.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803996.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

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CloneID	OBa45788
Clone ID Related Accession (Same CDS sequence)	XM_010805694.2 , XM_015463560.1
Accession Version	XM_010805694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803996.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805694.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805694.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463560.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319046.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

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CloneID	OBa45788
Clone ID Related Accession (Same CDS sequence)	XM_010805694.2 , XM_015463560.1
Accession Version	XM_015463560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319046.1
CDS	1918..7128
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463560.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463560.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_005207302.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005207359.2 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

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CloneID	OBa45789
Clone ID Related Accession (Same CDS sequence)	XM_005207302.3 , XM_015463562.1
Accession Version	XM_005207302.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207302.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005207359.2
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_005207302.3
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005207302.3

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463562.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319048.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa45789
Clone ID Related Accession (Same CDS sequence)	XM_005207302.3 , XM_015463562.1
Accession Version	XM_015463562.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319048.1
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463562.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463562.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805695.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803997.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

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CloneID	OBa45790
Clone ID Related Accession (Same CDS sequence)	XM_010805695.2 , XM_015463564.1
Accession Version	XM_010805695.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803997.1
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805695.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805695.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463564.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319050.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

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CloneID	OBa45790
Clone ID Related Accession (Same CDS sequence)	XM_010805695.2 , XM_015463564.1
Accession Version	XM_015463564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5211bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319050.1
CDS	1..5211
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463564.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463564.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGTGAGGG GCCCTTGCCC CTGGGGACCT GGCACCTCCT CCCACCTCTT CCAGGTTCCA 
CGCTCCCACG GGTCCCTTCC GGAAATTCCG AGCCTGCAGG GGGACCCTCC CCTCCCGCCC 
TGCCTCCTGC ACTCACTGCC CTCTGTAGTT CTGGACTCTC AGCCCCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805696.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803998.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

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CloneID	OBa45791
Clone ID Related Accession (Same CDS sequence)	XM_010805696.2 , XM_015463571.1
Accession Version	XM_010805696.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805696.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803998.1
CDS	1917..7094
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805696.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805696.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463571.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319057.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

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CloneID	OBa45791
Clone ID Related Accession (Same CDS sequence)	XM_010805696.2 , XM_015463571.1
Accession Version	XM_015463571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319057.1
CDS	1917..7094
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463571.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463571.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463572.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319058.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

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CloneID	OBa45792
Clone ID Related Accession (Same CDS sequence)	XM_015463572.1 , XM_010805697.2
Accession Version	XM_015463572.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319058.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463572.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463572.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805697.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803999.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

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CloneID	OBa45792
Clone ID Related Accession (Same CDS sequence)	XM_015463572.1 , XM_010805697.2
Accession Version	XM_010805697.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803999.1
CDS	1918..7095
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805697.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805697.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GTGAGGGGCC CTTGCCCCTG GGGACCTGGC 
ACCTCCTCCC ACCTCTTCCA GGTTCCACGC TCCCACGGGT CCCTTCCGGA AATTCCGAGC 
CTGCAGGGGG ACCCTCCCCT CCCGCCCTGC CTCCTGCACT CACTGCCCTC TGTAGTTCTG 
GACTCTCAGC CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_010805698.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010804000.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

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CloneID	OBa45793
Clone ID Related Accession (Same CDS sequence)	XM_010805698.2 , XM_015463576.1
Accession Version	XM_010805698.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103942.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805698.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC 
TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG 
GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT 
CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010804000.1
CDS	1917..7088
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITENAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_010805698.2
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805698.2

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC 
TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG 
GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT 
CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_015463576.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015319062.1 Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OBa45793
Clone ID Related Accession (Same CDS sequence)	XM_010805698.2 , XM_015463576.1
Accession Version	XM_015463576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644857.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC 
TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG 
GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT 
CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015319062.1
CDS	1917..7088
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITENAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQVRGPCPWGPGTSSHLFQVPRSHGSLPEIPSLQGDPPLPPCLLHSLPSVVLDSQPL

Target ORF information:

RefSeq Version	XM_015463576.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium channel, voltage-dependent, L type, alpha 1C subunit (CACNA1C), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463576.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGTGAGG GGCCCTTGCC CCTGGGGACC TGGCACCTCC 
TCCCACCTCT TCCAGGTTCC ACGCTCCCAC GGGTCCCTTC CGGAAATTCC GAGCCTGCAG 
GGGGACCCTC CCCTCCCGCC CTGCCTCCTG CACTCACTGC CCTCTGTAGT TCTGGACTCT 
CAGCCCCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992746.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848514.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X1, mRNA.

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CloneID	OBa234933
Clone ID Related Accession (Same CDS sequence)	XM_024992746.1
Accession Version	XM_024992746.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848514.1
CDS	1835..8338
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992746.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992746.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992747.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848515.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X2, mRNA.

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CloneID	OBa234934
Clone ID Related Accession (Same CDS sequence)	XM_024992747.1
Accession Version	XM_024992747.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848515.1
CDS	1836..8339
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992747.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992747.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992748.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848516.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X3, mRNA.

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CloneID	OBa234935
Clone ID Related Accession (Same CDS sequence)	XM_024992748.1
Accession Version	XM_024992748.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848516.1
CDS	1837..8340
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992748.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992748.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992749.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848517.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X4, mRNA.

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CloneID	OBa234936
Clone ID Related Accession (Same CDS sequence)	XM_024992749.1
Accession Version	XM_024992749.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848517.1
CDS	1836..8339
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992749.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992749.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992750.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848518.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X5, mRNA.

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CloneID	OBa234937
Clone ID Related Accession (Same CDS sequence)	XM_024992750.1
Accession Version	XM_024992750.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848518.1
CDS	1838..8341
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992750.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992750.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992752.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848520.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X6, mRNA.

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CloneID	OBa234938
Clone ID Related Accession (Same CDS sequence)	XM_024992752.1
Accession Version	XM_024992752.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848520.1
CDS	1838..8341
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992752.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992752.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992753.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848521.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X7, mRNA.

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CloneID	OBa234939
Clone ID Related Accession (Same CDS sequence)	XM_024992753.1
Accession Version	XM_024992753.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6504bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848521.1
CDS	1839..8342
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992753.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992753.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC CAGCTGAACA TACCCAATGC 
TCTCCCTTTA TGAACGCGGA AGAGAACTCT CGCATCTCCA TCACCTTCTT CCGCCTCTTC 
CGGGTCATGC GTCTGGTCAA GCTGCTGAGC CGCGGGGAGG GCATCCGGAC ACTGCTGTGG 
ACCTTCATCA AGTCCTTCCA GGCCCTACCC TATGTGGCCC TCCTGATCGT GATGCTGTTT 
TTCATCTACG CCGTGATCGG GATGCAGGTG TTTGGCAAAA TTGCCCTGAA TGACACGACA 
GAGATCAACA GGAACAACAA CTTCCAGACC TTCCCCCAGG CCGTGCTGCT CCTCTTCAGG 
TGCGCCACTG GGGAGGCCTG GCAGGACATC ATGCTGGCCT GCATGCCTGG CAAGAAGTGC 
GCCCCCGAGT CAGAGCCGGG CAACAGCACA GAGGGGGAGA CGCCCTGTGG CAGCAGCTTC 
GCCGTCTTCT ACTTCATCAG CTTCTACATG CTCTGCGCCT TCCTGATCAT CAACCTCTTC 
GTAGCCGTCA TCATGGACAA CTTTGACTAC CTGACGAGGG ACTGGTCCAT CCTGGGTCCC 
CACCACCTGG ACGAATTTAA GAGAATTTGG GCAGAGTATG ACCCTGAAGC CAAGGGACGC 
ATCAAACACC TGGATGTGGT GACCCTGCTC CGACGGATTC AGCCCCCCCT GGGTTTTGGG 
AAGCTGTGCC CTCACCGCGT GGCTTGCAAA CGCCTGGTCT CCATGAACAT GCCCCTGAAC 
AGCGACGGGA CTGTCATGTT CAATGCCACG CTGTTTGCCC TGGTCCGTAC AGCTCTGAGG 
ATCAAGACGG AAGGGAACCT GGAACAGGCC AATGAGGAGC TGCGGGCCAT CATCAAGAAG 
ATCTGGAAAC GGACCAGCAT GAAGCTGCTG GACCAAGTGG TGCCGCCCGC AGGAGATGAC 
GAGGTCACGG TGGGCAAGTT TTACGCTACT TTCCTGATCC AGGAGTATTT CCGGAAGTTC 
AAGAAGCGCA AAGAGCAAGG CCTGGTGGGC AAGCCCTCCC AGAGGAACGC CCTGTCCCTG 
CAGGCTGGCC TGCGCACCCT GCACGATCTG GGGCCCGAGA TCCGCCGGGC CATCTCTGGG 
GACCTGACCG CCGAGGAGGA GCTGGACAAG GCCATGAAGG AGGCTGTGTC TGCAGCCTCA 
GAAGATGACA TCTTCAGGAG GGCCGGCGGC CTGTTCGGCA ACCACGTGGG CTACTACCCG 
AGCGACGGCC GGGGCGCCTT CCCCCAGACC TTCACCACCC AGCGCCCCCT GCACATCAGC 
AAGGCAGGCA ACAGCCAGGG GGACACCGAG TCCCCCTCCC ACGAGAAACT GGTGGACTCC 
ACCTTCACCC CCAGCAGCTA CTCATCCACG GGCTCCAACG CCAACATCAA CAACGCCAAC 
AACACCGCCC TGGGCCGCTG CCCCCGCCCC GCGCTCCACT CCGGTGCTGT CAGCACCTTG 
GAGGCCCACG GGCCCCCCTT GTCCCCTGCC ATCCGGGTGC AGGAGGCCGC GTGGAGGCAC 
AGCTCCCAGA GGTGCCCCTC CCGGGACAGC CCGATAGCCA TGCTGTGTCA GGAAGAGGCC 
TCTGGGGACG AGACCTGTGC CGTGCACGTG GACGAGAACG CAGAGCACAG CAGCGAGCCC 
AGCTTGGTCT CCACGGAGAT GCTCTCCTAC CAGGATGACG AGAATCGGCA GCTGACTCCC 
CCCGAGGAGG ACAGGAGGGA CATCCGTCTG TCCCCAAAGA GGGGCTTCCT GCGCTCCGCC 
TCCCTAGGTC GAAGGGCCTC CTTCCACCTT GAATGTCTGA AGCGACAGAA GAATCCTGGG 
GGAGACGTCT CTCAGAACAC AGTCCTGCCT TTGCACCTGG TTCACCATCA GGCGCTGGCA 
GTGGCGGGTC TGAGCCCCCT CCTGCAGAGA AGCCATCCCC CTGGGACCCT CCCCCCACCA 
CGCCTCACAC CCCCCGCCAC ACCTGGCCCA GCCTGGCCTC CGCGGCCTGT GCCCACCCTG 
CGGCTGGAAG GGGCCGAGTC CAGCGACAAG CTCACCAGCA GCTTCCCATC CATCCACTGC 
GACCCCCACA TCGGGGAGCC CACGCCCTGC GGGGTTGTCG GCACCCCCCG GAGGGCCCGG 
CCCGTGTCAC TCACTGTGCC CAGCCCGGCA GGGCCCCAGG GCCGTCCGTT CCATGGCAGT 
GCCAGCAGCC TGGTGGAGGC GGTCTTGATC TCGGAAGGAT TGGGGCAGTT TGCTCAGGAC 
CCCAGGTTCC TCGAGGCCAC CACGCAGGAG CTGGCCGATG CCTGCGACAT GACCATTGAG 
GAGATGGAGA GCGCGGCTGA CGACATCCTC AGCGGGGGCG CCGGGCAGAG CCCCAACGGC 
ACCCTGCTCC CGTGTGCAAA CTGCAGGGAC CCGGGGCCGG ACCGAGCGGG GGGTGTGGAG 
GACGCAGCCT GGGCGCCCAG CGCCGAGCCC CGCCAGGGCG CAGAGGAGCC CCGGGACAGC 
AGGGCCTTCG CCAGCGGCCT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992754.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848522.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X8, mRNA.

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CloneID	OBa234940
Clone ID Related Accession (Same CDS sequence)	XM_024992754.1
Accession Version	XM_024992754.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT 
GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG 
ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT 
GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC 
GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA 
GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC 
ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC 
GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC 
CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC 
CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG 
GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG 
GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG 
GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA 
GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC 
GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG 
GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC 
ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG 
GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC 
CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG 
TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC 
AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG 
TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG 
GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG 
CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA 
GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC 
TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848522.1
CDS	1835..8332
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992754.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992754.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT 
GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG 
ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT 
GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC 
GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA 
GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC 
ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC 
GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC 
CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC 
CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG 
GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG 
GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG 
GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA 
GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC 
GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG 
GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC 
ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG 
GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC 
CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG 
TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC 
AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG 
TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG 
GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG 
CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA 
GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC 
TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992755.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848523.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X9, mRNA.

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CloneID	OBa234941
Clone ID Related Accession (Same CDS sequence)	XM_024992755.1
Accession Version	XM_024992755.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT 
GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG 
ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT 
GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC 
GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA 
GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC 
ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC 
GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC 
CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC 
CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG 
GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG 
GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG 
GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA 
GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC 
GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG 
GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC 
ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG 
GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC 
CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG 
TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC 
AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG 
TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG 
GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG 
CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA 
GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC 
TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848523.1
CDS	1838..8335
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992755.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992755.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGCCAGCTG AACATACCCA ATGCTCTCCC 
TTTATGAACG CGGAAGAGAA CTCTCGCATC TCCATCACCT TCTTCCGCCT CTTCCGGGTC 
ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG GAGGGCATCC GGACACTGCT GTGGACCTTC 
ATCAAGTCCT TCCAGGCCCT ACCCTATGTG GCCCTCCTGA TCGTGATGCT GTTTTTCATC 
TACGCCGTGA TCGGGATGCA GGTGTTTGGC AAAATTGCCC TGAATGACAC GACAGAGATC 
AACAGGAACA ACAACTTCCA GACCTTCCCC CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC 
ACTGGGGAGG CCTGGCAGGA CATCATGCTG GCCTGCATGC CTGGCAAGAA GTGCGCCCCC 
GAGTCAGAGC CGGGCAACAG CACAGAGGGG GAGACGCCCT GTGGCAGCAG CTTCGCCGTC 
TTCTACTTCA TCAGCTTCTA CATGCTCTGC GCCTTCCTGA TCATCAACCT CTTCGTAGCC 
GTCATCATGG ACAACTTTGA CTACCTGACG AGGGACTGGT CCATCCTGGG TCCCCACCAC 
CTGGACGAAT TTAAGAGAAT TTGGGCAGAG TATGACCCTG AAGCCAAGGG ACGCATCAAA 
CACCTGGATG TGGTGACCCT GCTCCGACGG ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG 
TGCCCTCACC GCGTGGCTTG CAAACGCCTG GTCTCCATGA ACATGCCCCT GAACAGCGAC 
GGGACTGTCA TGTTCAATGC CACGCTGTTT GCCCTGGTCC GTACAGCTCT GAGGATCAAG 
ACGGAAGGGA ACCTGGAACA GGCCAATGAG GAGCTGCGGG CCATCATCAA GAAGATCTGG 
AAACGGACCA GCATGAAGCT GCTGGACCAA GTGGTGCCGC CCGCAGGAGA TGACGAGGTC 
ACGGTGGGCA AGTTTTACGC TACTTTCCTG ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG 
CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT 
GGCCTGCGCA CCCTGCACGA TCTGGGGCCC GAGATCCGCC GGGCCATCTC TGGGGACCTG 
ACCGCCGAGG AGGAGCTGGA CAAGGCCATG AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT 
GACATCTTCA GGAGGGCCGG CGGCCTGTTC GGCAACCACG TGGGCTACTA CCCGAGCGAC 
GGCCGGGGCG CCTTCCCCCA GACCTTCACC ACCCAGCGCC CCCTGCACAT CAGCAAGGCA 
GGCAACAGCC AGGGGGACAC CGAGTCCCCC TCCCACGAGA AACTGGTGGA CTCCACCTTC 
ACCCCCAGCA GCTACTCATC CACGGGCTCC AACGCCAACA TCAACAACGC CAACAACACC 
GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC CACTCCGGTG CTGTCAGCAC CTTGGAGGCC 
CACGGGCCCC CCTTGTCCCC TGCCATCCGG GTGCAGGAGG CCGCGTGGAG GCACAGCTCC 
CAGAGGTGCC CCTCCCGGGA CAGCCCGATA GCCATGCTGT GTCAGGAAGA GGCCTCTGGG 
GACGAGACCT GTGCCGTGCA CGTGGACGAG AACGCAGAGC ACAGCAGCGA GCCCAGCTTG 
GTCTCCACGG AGATGCTCTC CTACCAGGAT GACGAGAATC GGCAGCTGAC TCCCCCCGAG 
GAGGACAGGA GGGACATCCG TCTGTCCCCA AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA 
GGTCGAAGGG CCTCCTTCCA CCTTGAATGT CTGAAGCGAC AGAAGAATCC TGGGGGAGAC 
GTCTCTCAGA ACACAGTCCT GCCTTTGCAC CTGGTTCACC ATCAGGCGCT GGCAGTGGCG 
GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT CCCCCTGGGA CCCTCCCCCC ACCACGCCTC 
ACACCCCCCG CCACACCTGG CCCAGCCTGG CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG 
GAAGGGGCCG AGTCCAGCGA CAAGCTCACC AGCAGCTTCC CATCCATCCA CTGCGACCCC 
CACATCGGGG AGCCCACGCC CTGCGGGGTT GTCGGCACCC CCCGGAGGGC CCGGCCCGTG 
TCACTCACTG TGCCCAGCCC GGCAGGGCCC CAGGGCCGTC CGTTCCATGG CAGTGCCAGC 
AGCCTGGTGG AGGCGGTCTT GATCTCGGAA GGATTGGGGC AGTTTGCTCA GGACCCCAGG 
TTCCTCGAGG CCACCACGCA GGAGCTGGCC GATGCCTGCG ACATGACCAT TGAGGAGATG 
GAGAGCGCGG CTGACGACAT CCTCAGCGGG GGCGCCGGGC AGAGCCCCAA CGGCACCCTG 
CTCCCGTGTG CAAACTGCAG GGACCCGGGG CCGGACCGAG CGGGGGGTGT GGAGGACGCA 
GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC 
TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992756.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848524.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X10, mRNA.

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CloneID	OBa234942
Clone ID Related Accession (Same CDS sequence)	XM_024992756.1
Accession Version	XM_024992756.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGCTGGCCTG 
CGCACCCTGC ACGATCTGGG GCCCGAGATC CGCCGGGCCA TCTCTGGGGA CCTGACCGCC 
GAGGAGGAGC TGGACAAGGC CATGAAGGAG GCTGTGTCTG CAGCCTCAGA AGATGACATC 
TTCAGGAGGG CCGGCGGCCT GTTCGGCAAC CACGTGGGCT ACTACCCGAG CGACGGCCGG 
GGCGCCTTCC CCCAGACCTT CACCACCCAG CGCCCCCTGC ACATCAGCAA GGCAGGCAAC 
AGCCAGGGGG ACACCGAGTC CCCCTCCCAC GAGAAACTGG TGGACTCCAC CTTCACCCCC 
AGCAGCTACT CATCCACGGG CTCCAACGCC AACATCAACA ACGCCAACAA CACCGCCCTG 
GGCCGCTGCC CCCGCCCCGC GCTCCACTCC GGTGCTGTCA GCACCTTGGA GGCCCACGGG 
CCCCCCTTGT CCCCTGCCAT CCGGGTGCAG GAGGCCGCGT GGAGGCACAG CTCCCAGAGG 
TGCCCCTCCC GGGACAGCCC GATAGCCATG CTGTGTCAGG AAGAGGCCTC TGGGGACGAG 
ACCTGTGCCG TGCACGTGGA CGAGAACGCA GAGCACAGCA GCGAGCCCAG CTTGGTCTCC 
ACGGAGATGC TCTCCTACCA GGATGACGAG AATCGGCAGC TGACTCCCCC CGAGGAGGAC 
AGGAGGGACA TCCGTCTGTC CCCAAAGAGG GGCTTCCTGC GCTCCGCCTC CCTAGGTCGA 
AGGGCCTCCT TCCACCTTGA ATGTCTGAAG CGACAGAAGA ATCCTGGGGG AGACGTCTCT 
CAGAACACAG TCCTGCCTTT GCACCTGGTT CACCATCAGG CGCTGGCAGT GGCGGGTCTG 
AGCCCCCTCC TGCAGAGAAG CCATCCCCCT GGGACCCTCC CCCCACCACG CCTCACACCC 
CCCGCCACAC CTGGCCCAGC CTGGCCTCCG CGGCCTGTGC CCACCCTGCG GCTGGAAGGG 
GCCGAGTCCA GCGACAAGCT CACCAGCAGC TTCCCATCCA TCCACTGCGA CCCCCACATC 
GGGGAGCCCA CGCCCTGCGG GGTTGTCGGC ACCCCCCGGA GGGCCCGGCC CGTGTCACTC 
ACTGTGCCCA GCCCGGCAGG GCCCCAGGGC CGTCCGTTCC ATGGCAGTGC CAGCAGCCTG 
GTGGAGGCGG TCTTGATCTC GGAAGGATTG GGGCAGTTTG CTCAGGACCC CAGGTTCCTC 
GAGGCCACCA CGCAGGAGCT GGCCGATGCC TGCGACATGA CCATTGAGGA GATGGAGAGC 
GCGGCTGACG ACATCCTCAG CGGGGGCGCC GGGCAGAGCC CCAACGGCAC CCTGCTCCCG 
TGTGCAAACT GCAGGGACCC GGGGCCGGAC CGAGCGGGGG GTGTGGAGGA CGCAGCCTGG 
GCGCCCAGCG CCGAGCCCCG CCAGGGCGCA GAGGAGCCCC GGGACAGCAG GGCCTTCGCC 
AGCGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848524.1
CDS	1836..8327
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992756.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992756.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGAAGACGA CCCCTCGCCC 
TGTGCCCTGG AGACAGGCCA CGGGCGGCAG TGCCAGAATG GCACGGTGTG CAAACCAGGC 
TGGGATGGAC CCAAGCACGG CATCACCAAT TTCGACAACT TCGCCTTCGC CATGCTGACT 
GTGTTCCAGT GCATCACCAT GGAGGGCTGG ACCGACGTCC TCTACTGGGT CAATGATGCC 
GTGGGAAGGG ACTGGCCCTG GATCTATTTT GTCACATTAA TCATCATAGG TTCATTCTTT 
GTTCTTAACT TGGTTCTCGG TGTCCTTAGC GGAGAGTTCT CCAAAGAGCG AGAGAAGGCC 
AAGGCCCGGG GAGACTTCCA GAAGCTGCGA GAGAAGCAGC AGCTGGAGGA GGACCTGAAG 
GGCTACCTGG ACTGGATCAC GCAGGCCGAG GACATCGACC CCGAGAATGA GGACGAGGGC 
CTGGACGAGG AGAAACCCCG AAACATGAGC ATGCCCACCA GTGAGACCGA GTCTGTCAAC 
ACCGAAAACG TGGCTGGAGG GGACATCGAG GGAGAGAGCT GCGGGGCCAG GCTGGCCCAC 
CGGATCTCCA AGTCAAAGTT CAGCCGCTAC TGGCGCCGGT GGAATCGGTT CTGCAGGAGG 
AAGTGCCGCG CGGCCGTGAA GTCCAGCATC TTCTACTGGC TGGTCATCTT CCTGGTTTTC 
CTCAACACAC TCACCATCGC CTCCGAGCAC TACAACCAGC CCCACTGGCT CACGGAAGTC 
CAAGACACGG CCAACAAGGC GCTGCTGGCC CTGTTCACGG CGGAGATGCT GCTCAAGATG 
TACAGCCTGG GCCTGCAGGC CTACTTCGTG TCGCTCTTCA ACCGCTTCGA CTGCTTCATC 
GTGTGCGGGG GCATCCTGGA GACCATCCTG GTGGAGACCA AGATCATGTC GCCGTTGGGC 
ATCTCCGTGC TGCGTTGCGT GCGCCTCCTT CGGATATTTA AGATCACAAG GTACTGGAAC 
TCCCTGAGCA ACCTGGTGGC ATCCCTGCTG AACTCAGTGC GCTCCATTGC CTCGCTGCTG 
CTGCTGCTCT TCCTCTTCAT CATCATCTTC TCCCTCCTGG GGATGCAGCT CTTTGGGGGG 
AAGTTCAACT TTGATGAGAT GCAGACCCGG AGGAGCACCT TTGACAACTT CCCGCAGTCC 
CTCCTCACCG TGTTTCAGAT CCTGACCGGG GAGGACTGGA ATTCGGTGAT GTATGATGGG 
ATCATGGCTT ATGGCGGCCC CTCTTTTCCA GGGATGTTAG TCTGTATTTA CTTCATCATC 
CTCTTCATCT GTGGAAATTA TATCCTACTG AACGTGTTCT TGGCCATTGC TGTGGACAAC 
CTGGCTGACG CGGAGAGCCT CACATCTGCC CAGAAGGAGG AGGAAGAGGA GAAGGAGAGG 
AAGAAGCTAG CCAGGACCGC CAGCCCAGAG AAGAAGCAAG AGGTGGTAGA GAAGCCGGCC 
GTGGAGGAGA CCAAGGAGGA GAAAATCGAG CTGAAATCCA TCACGGCAGA TGGAGAGTCC 
CCACCCGCCA CCAAGATCAA CATGGATGAC CTCCAGCCCA ACGAGAATGA GGACAAGAGC 
TCCTACCCCA ACCAGGAAAC CACAGGAGAA GAGGACGAGG AGGAGCCCGA GATGCCCGTG 
GGCCCCCGCC CGCGCCCCCT CTCTGAGCTG CACCTGAAGG AGAAGGCAGT GCCCATGCCG 
GAAGCCAGCG CCTTCTTCAT CTTCAGCCCC AGCAACAGGT TCCGCCTCCA GTGCCATCGC 
ATCGTCAATG ACTCCATCTT CACCAACCTG ATCCTCTTCT TCATCCTGCT CAGCAGCATC 
TCCCTGGCCG CCGAGGACCC CGTGCAGCAC ACCTCCTTCA GGAACCACAT TCTGTTTTAT 
TTTGACATTG TTTTCACTAC CATTTTCACC ATTGAGATTG CCCTGAAGAT GACGGCCTAC 
GGGGCCTTCC TGCACAAGGG CTCCTTCTGC CGCAACTACT TCAACATCCT GGACCTGCTG 
GTGGTCAGTG TGTCCCTCAT CTCCTTCGGC ATCCAGTCCA GTGCCATCAA CGTGGTGAAG 
ATCCTGCGAG TCCTGAGGGT GCTCCGGCCA CTGAGGGCCA TCAACAGGGC CAAGGGGCTG 
AAGCACGTCG TGCAGTGCGT GTTCGTGGCC ATCCGCACCA TCGGGAACAT TGTGATCGTC 
ACCACGCTGC TCCAGTTCAT GTTCGCCTGC ATTGGGGTCC AGCTCTTCAA GGGAAAGCTC 
TACACCTGTT CGGACAGTTC CAAACAGACA GAGGCTGAAT GCAAGGGGAA CTACATAACA 
TACAAGGATG GGGAGGTGGA CCACCCCATC ATCCAGCCCC GCAGCTGGGA GAACAGCAAG 
TTTGACTTTG ACAATGTCCT GGCAGCCATG ATGGCCCTGT TCACCGTCTC CACCTTCGAG 
GGATGGCCAG AGTTGCTGTA CCGCTCCATC GACTCTCACA CGGAGGACAA GGGTCCCATA 
TACAACTACC GCGTGGAGAT CTCCGTCTTC TTCATCATCT ACATCATCAT CATTGCCTTC 
TTCATGATGA ACATCTTCGT GGGTTTCGTC ATCGTCACCT TCCAGGAGCA GGGGGAGCAG 
GAATACAAGA ACTGCGAGCT GGACAAGAAC CAGCGGCAGT GTGTGGAGTA TGCCCTCAAG 
GCCCGGCCCC TGCGGAGGTA CATCCCGAAA AACCAGCACC AGTACAAGGT GTGGTACGTG 
GTCAACTCCA CCTACTTCGA GTACCTGATG TTCGTTCTCA TTCTGCTCAA CACCATCTGC 
CTGGCCATGC AGCACTACGG TCAGAGCTGT CTGTTCAAAA TCGCCATGAA CATCCTCAAC 
ATGCTCTTCA CCGGCCTCTT CACAGTGGAG ATGGTCCTGA AGCTCATCGC CTTCAAGCCC 
AAGCACTATT TCTGTGATGC ATGGAACACA TTTGACGCCT TGATTGTTGT GGGTAGCATT 
GTTGATATAG CCATCACCGA GGTAAACCCA GCTGAACATA CCCAATGCTC TCCCTTTATG 
AACGCGGAAG AGAACTCTCG CATCTCCATC ACCTTCTTCC GCCTCTTCCG GGTCATGCGT 
CTGGTCAAGC TGCTGAGCCG CGGGGAGGGC ATCCGGACAC TGCTGTGGAC CTTCATCAAG 
TCCTTCCAGG CCCTACCCTA TGTGGCCCTC CTGATCGTGA TGCTGTTTTT CATCTACGCC 
GTGATCGGGA TGCAGGTGTT TGGCAAAATT GCCCTGAATG ACACGACAGA GATCAACAGG 
AACAACAACT TCCAGACCTT CCCCCAGGCC GTGCTGCTCC TCTTCAGGTG CGCCACTGGG 
GAGGCCTGGC AGGACATCAT GCTGGCCTGC ATGCCTGGCA AGAAGTGCGC CCCCGAGTCA 
GAGCCGGGCA ACAGCACAGA GGGGGAGACG CCCTGTGGCA GCAGCTTCGC CGTCTTCTAC 
TTCATCAGCT TCTACATGCT CTGCGCCTTC CTGATCATCA ACCTCTTCGT AGCCGTCATC 
ATGGACAACT TTGACTACCT GACGAGGGAC TGGTCCATCC TGGGTCCCCA CCACCTGGAC 
GAATTTAAGA GAATTTGGGC AGAGTATGAC CCTGAAGCCA AGGGACGCAT CAAACACCTG 
GATGTGGTGA CCCTGCTCCG ACGGATTCAG CCCCCCCTGG GTTTTGGGAA GCTGTGCCCT 
CACCGCGTGG CTTGCAAACG CCTGGTCTCC ATGAACATGC CCCTGAACAG CGACGGGACT 
GTCATGTTCA ATGCCACGCT GTTTGCCCTG GTCCGTACAG CTCTGAGGAT CAAGACGGAA 
GGGAACCTGG AACAGGCCAA TGAGGAGCTG CGGGCCATCA TCAAGAAGAT CTGGAAACGG 
ACCAGCATGA AGCTGCTGGA CCAAGTGGTG CCGCCCGCAG GAGATGACGA GGTCACGGTG 
GGCAAGTTTT ACGCTACTTT CCTGATCCAG GAGTATTTCC GGAAGTTCAA GAAGCGCAAA 
GAGCAAGGCC TGGTGGGCAA GCCCTCCCAG AGGAACGCCC TGTCCCTGCA GGCTGGCCTG 
CGCACCCTGC ACGATCTGGG GCCCGAGATC CGCCGGGCCA TCTCTGGGGA CCTGACCGCC 
GAGGAGGAGC TGGACAAGGC CATGAAGGAG GCTGTGTCTG CAGCCTCAGA AGATGACATC 
TTCAGGAGGG CCGGCGGCCT GTTCGGCAAC CACGTGGGCT ACTACCCGAG CGACGGCCGG 
GGCGCCTTCC CCCAGACCTT CACCACCCAG CGCCCCCTGC ACATCAGCAA GGCAGGCAAC 
AGCCAGGGGG ACACCGAGTC CCCCTCCCAC GAGAAACTGG TGGACTCCAC CTTCACCCCC 
AGCAGCTACT CATCCACGGG CTCCAACGCC AACATCAACA ACGCCAACAA CACCGCCCTG 
GGCCGCTGCC CCCGCCCCGC GCTCCACTCC GGTGCTGTCA GCACCTTGGA GGCCCACGGG 
CCCCCCTTGT CCCCTGCCAT CCGGGTGCAG GAGGCCGCGT GGAGGCACAG CTCCCAGAGG 
TGCCCCTCCC GGGACAGCCC GATAGCCATG CTGTGTCAGG AAGAGGCCTC TGGGGACGAG 
ACCTGTGCCG TGCACGTGGA CGAGAACGCA GAGCACAGCA GCGAGCCCAG CTTGGTCTCC 
ACGGAGATGC TCTCCTACCA GGATGACGAG AATCGGCAGC TGACTCCCCC CGAGGAGGAC 
AGGAGGGACA TCCGTCTGTC CCCAAAGAGG GGCTTCCTGC GCTCCGCCTC CCTAGGTCGA 
AGGGCCTCCT TCCACCTTGA ATGTCTGAAG CGACAGAAGA ATCCTGGGGG AGACGTCTCT 
CAGAACACAG TCCTGCCTTT GCACCTGGTT CACCATCAGG CGCTGGCAGT GGCGGGTCTG 
AGCCCCCTCC TGCAGAGAAG CCATCCCCCT GGGACCCTCC CCCCACCACG CCTCACACCC 
CCCGCCACAC CTGGCCCAGC CTGGCCTCCG CGGCCTGTGC CCACCCTGCG GCTGGAAGGG 
GCCGAGTCCA GCGACAAGCT CACCAGCAGC TTCCCATCCA TCCACTGCGA CCCCCACATC 
GGGGAGCCCA CGCCCTGCGG GGTTGTCGGC ACCCCCCGGA GGGCCCGGCC CGTGTCACTC 
ACTGTGCCCA GCCCGGCAGG GCCCCAGGGC CGTCCGTTCC ATGGCAGTGC CAGCAGCCTG 
GTGGAGGCGG TCTTGATCTC GGAAGGATTG GGGCAGTTTG CTCAGGACCC CAGGTTCCTC 
GAGGCCACCA CGCAGGAGCT GGCCGATGCC TGCGACATGA CCATTGAGGA GATGGAGAGC 
GCGGCTGACG ACATCCTCAG CGGGGGCGCC GGGCAGAGCC CCAACGGCAC CCTGCTCCCG 
TGTGCAAACT GCAGGGACCC GGGGCCGGAC CGAGCGGGGG GTGTGGAGGA CGCAGCCTGG 
GCGCCCAGCG CCGAGCCCCG CCAGGGCGCA GAGGAGCCCC GGGACAGCAG GGCCTTCGCC 
AGCGGCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992757.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848525.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X11, mRNA.

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CloneID	OBa234943
Clone ID Related Accession (Same CDS sequence)	XM_024992757.1
Accession Version	XM_024992757.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848525.1
CDS	1835..8305
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992757.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992757.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992758.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848526.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X12, mRNA.

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CloneID	OBa234944
Clone ID Related Accession (Same CDS sequence)	XM_024992758.1
Accession Version	XM_024992758.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848526.1
CDS	1836..8306
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWMQDAMGYELPWVYFVSLVIFGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992758.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992758.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
ATGCAGGACG CTATGGGCTA CGAGTTACCC TGGGTGTATT TTGTCAGTCT GGTCATCTTC 
GGATCCTTCT TCGTTCTAAA TCTGGTTCTT GGTGTGTTGA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992759.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848527.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X13, mRNA.

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CloneID	OBa234945
Clone ID Related Accession (Same CDS sequence)	XM_024992759.1
Accession Version	XM_024992759.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848527.1
CDS	1836..8306
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992759.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992759.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992760.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848528.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X14, mRNA.

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CloneID	OBa234946
Clone ID Related Accession (Same CDS sequence)	XM_024992760.1
Accession Version	XM_024992760.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848528.1
CDS	1837..8307
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992760.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992760.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGGTAAACA ACGCGGAAGA GAACTCTCGC 
ATCTCCATCA CCTTCTTCCG CCTCTTCCGG GTCATGCGTC TGGTCAAGCT GCTGAGCCGC 
GGGGAGGGCA TCCGGACACT GCTGTGGACC TTCATCAAGT CCTTCCAGGC CCTACCCTAT 
GTGGCCCTCC TGATCGTGAT GCTGTTTTTC ATCTACGCCG TGATCGGGAT GCAGGTGTTT 
GGCAAAATTG CCCTGAATGA CACGACAGAG ATCAACAGGA ACAACAACTT CCAGACCTTC 
CCCCAGGCCG TGCTGCTCCT CTTCAGGTGC GCCACTGGGG AGGCCTGGCA GGACATCATG 
CTGGCCTGCA TGCCTGGCAA GAAGTGCGCC CCCGAGTCAG AGCCGGGCAA CAGCACAGAG 
GGGGAGACGC CCTGTGGCAG CAGCTTCGCC GTCTTCTACT TCATCAGCTT CTACATGCTC 
TGCGCCTTCC TGATCATCAA CCTCTTCGTA GCCGTCATCA TGGACAACTT TGACTACCTG 
ACGAGGGACT GGTCCATCCT GGGTCCCCAC CACCTGGACG AATTTAAGAG AATTTGGGCA 
GAGTATGACC CTGAAGCCAA GGGACGCATC AAACACCTGG ATGTGGTGAC CCTGCTCCGA 
CGGATTCAGC CCCCCCTGGG TTTTGGGAAG CTGTGCCCTC ACCGCGTGGC TTGCAAACGC 
CTGGTCTCCA TGAACATGCC CCTGAACAGC GACGGGACTG TCATGTTCAA TGCCACGCTG 
TTTGCCCTGG TCCGTACAGC TCTGAGGATC AAGACGGAAG GGAACCTGGA ACAGGCCAAT 
GAGGAGCTGC GGGCCATCAT CAAGAAGATC TGGAAACGGA CCAGCATGAA GCTGCTGGAC 
CAAGTGGTGC CGCCCGCAGG AGATGACGAG GTCACGGTGG GCAAGTTTTA CGCTACTTTC 
CTGATCCAGG AGTATTTCCG GAAGTTCAAG AAGCGCAAAG AGCAAGGCCT GGTGGGCAAG 
CCCTCCCAGA GGAACGCCCT GTCCCTGCAG GCTGGCCTGC GCACCCTGCA CGATCTGGGG 
CCCGAGATCC GCCGGGCCAT CTCTGGGGAC CTGACCGCCG AGGAGGAGCT GGACAAGGCC 
ATGAAGGAGG CTGTGTCTGC AGCCTCAGAA GATGACATCT TCAGGAGGGC CGGCGGCCTG 
TTCGGCAACC ACGTGGGCTA CTACCCGAGC GACGGCCGGG GCGCCTTCCC CCAGACCTTC 
ACCACCCAGC GCCCCCTGCA CATCAGCAAG GCAGGCAACA GCCAGGGGGA CACCGAGTCC 
CCCTCCCACG AGAAACTGGT GGACTCCACC TTCACCCCCA GCAGCTACTC ATCCACGGGC 
TCCAACGCCA ACATCAACAA CGCCAACAAC ACCGCCCTGG GCCGCTGCCC CCGCCCCGCG 
CTCCACTCCG GTGCTGTCAG CACCTTGGAG GCCCACGGGC CCCCCTTGTC CCCTGCCATC 
CGGGTGCAGG AGGCCGCGTG GAGGCACAGC TCCCAGAGGT GCCCCTCCCG GGACAGCCCG 
ATAGCCATGC TGTGTCAGGA AGAGGCCTCT GGGGACGAGA CCTGTGCCGT GCACGTGGAC 
GAGAACGCAG AGCACAGCAG CGAGCCCAGC TTGGTCTCCA CGGAGATGCT CTCCTACCAG 
GATGACGAGA ATCGGCAGCT GACTCCCCCC GAGGAGGACA GGAGGGACAT CCGTCTGTCC 
CCAAAGAGGG GCTTCCTGCG CTCCGCCTCC CTAGGTCGAA GGGCCTCCTT CCACCTTGAA 
TGTCTGAAGC GACAGAAGAA TCCTGGGGGA GACGTCTCTC AGAACACAGT CCTGCCTTTG 
CACCTGGTTC ACCATCAGGC GCTGGCAGTG GCGGGTCTGA GCCCCCTCCT GCAGAGAAGC 
CATCCCCCTG GGACCCTCCC CCCACCACGC CTCACACCCC CCGCCACACC TGGCCCAGCC 
TGGCCTCCGC GGCCTGTGCC CACCCTGCGG CTGGAAGGGG CCGAGTCCAG CGACAAGCTC 
ACCAGCAGCT TCCCATCCAT CCACTGCGAC CCCCACATCG GGGAGCCCAC GCCCTGCGGG 
GTTGTCGGCA CCCCCCGGAG GGCCCGGCCC GTGTCACTCA CTGTGCCCAG CCCGGCAGGG 
CCCCAGGGCC GTCCGTTCCA TGGCAGTGCC AGCAGCCTGG TGGAGGCGGT CTTGATCTCG 
GAAGGATTGG GGCAGTTTGC TCAGGACCCC AGGTTCCTCG AGGCCACCAC GCAGGAGCTG 
GCCGATGCCT GCGACATGAC CATTGAGGAG ATGGAGAGCG CGGCTGACGA CATCCTCAGC 
GGGGGCGCCG GGCAGAGCCC CAACGGCACC CTGCTCCCGT GTGCAAACTG CAGGGACCCG 
GGGCCGGACC GAGCGGGGGG TGTGGAGGAC GCAGCCTGGG CGCCCAGCGC CGAGCCCCGC 
CAGGGCGCAG AGGAGCCCCG GGACAGCAGG GCCTTCGCCA GCGGCCTGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992761.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848529.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X15, mRNA.

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CloneID	OBa234947
Clone ID Related Accession (Same CDS sequence)	XM_024992761.1
Accession Version	XM_024992761.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6465bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGCTGGC CTGCGCACCC TGCACGATCT GGGGCCCGAG 
ATCCGCCGGG CCATCTCTGG GGACCTGACC GCCGAGGAGG AGCTGGACAA GGCCATGAAG 
GAGGCTGTGT CTGCAGCCTC AGAAGATGAC ATCTTCAGGA GGGCCGGCGG CCTGTTCGGC 
AACCACGTGG GCTACTACCC GAGCGACGGC CGGGGCGCCT TCCCCCAGAC CTTCACCACC 
CAGCGCCCCC TGCACATCAG CAAGGCAGGC AACAGCCAGG GGGACACCGA GTCCCCCTCC 
CACGAGAAAC TGGTGGACTC CACCTTCACC CCCAGCAGCT ACTCATCCAC GGGCTCCAAC 
GCCAACATCA ACAACGCCAA CAACACCGCC CTGGGCCGCT GCCCCCGCCC CGCGCTCCAC 
TCCGGTGCTG TCAGCACCTT GGAGGCCCAC GGGCCCCCCT TGTCCCCTGC CATCCGGGTG 
CAGGAGGCCG CGTGGAGGCA CAGCTCCCAG AGGTGCCCCT CCCGGGACAG CCCGATAGCC 
ATGCTGTGTC AGGAAGAGGC CTCTGGGGAC GAGACCTGTG CCGTGCACGT GGACGAGAAC 
GCAGAGCACA GCAGCGAGCC CAGCTTGGTC TCCACGGAGA TGCTCTCCTA CCAGGATGAC 
GAGAATCGGC AGCTGACTCC CCCCGAGGAG GACAGGAGGG ACATCCGTCT GTCCCCAAAG 
AGGGGCTTCC TGCGCTCCGC CTCCCTAGGT CGAAGGGCCT CCTTCCACCT TGAATGTCTG 
AAGCGACAGA AGAATCCTGG GGGAGACGTC TCTCAGAACA CAGTCCTGCC TTTGCACCTG 
GTTCACCATC AGGCGCTGGC AGTGGCGGGT CTGAGCCCCC TCCTGCAGAG AAGCCATCCC 
CCTGGGACCC TCCCCCCACC ACGCCTCACA CCCCCCGCCA CACCTGGCCC AGCCTGGCCT 
CCGCGGCCTG TGCCCACCCT GCGGCTGGAA GGGGCCGAGT CCAGCGACAA GCTCACCAGC 
AGCTTCCCAT CCATCCACTG CGACCCCCAC ATCGGGGAGC CCACGCCCTG CGGGGTTGTC 
GGCACCCCCC GGAGGGCCCG GCCCGTGTCA CTCACTGTGC CCAGCCCGGC AGGGCCCCAG 
GGCCGTCCGT TCCATGGCAG TGCCAGCAGC CTGGTGGAGG CGGTCTTGAT CTCGGAAGGA 
TTGGGGCAGT TTGCTCAGGA CCCCAGGTTC CTCGAGGCCA CCACGCAGGA GCTGGCCGAT 
GCCTGCGACA TGACCATTGA GGAGATGGAG AGCGCGGCTG ACGACATCCT CAGCGGGGGC 
GCCGGGCAGA GCCCCAACGG CACCCTGCTC CCGTGTGCAA ACTGCAGGGA CCCGGGGCCG 
GACCGAGCGG GGGGTGTGGA GGACGCAGCC TGGGCGCCCA GCGCCGAGCC CCGCCAGGGC 
GCAGAGGAGC CCCGGGACAG CAGGGCCTTC GCCAGCGGCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848529.1
CDS	1835..8299
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITENAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992761.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992761.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGCACTA TTTCTGTGAT GCATGGAACA CATTTGACGC CTTGATTGTT 
GTGGGTAGCA TTGTTGATAT AGCCATCACC GAGAACGCGG AAGAGAACTC TCGCATCTCC 
ATCACCTTCT TCCGCCTCTT CCGGGTCATG CGTCTGGTCA AGCTGCTGAG CCGCGGGGAG 
GGCATCCGGA CACTGCTGTG GACCTTCATC AAGTCCTTCC AGGCCCTACC CTATGTGGCC 
CTCCTGATCG TGATGCTGTT TTTCATCTAC GCCGTGATCG GGATGCAGGT GTTTGGCAAA 
ATTGCCCTGA ATGACACGAC AGAGATCAAC AGGAACAACA ACTTCCAGAC CTTCCCCCAG 
GCCGTGCTGC TCCTCTTCAG GTGCGCCACT GGGGAGGCCT GGCAGGACAT CATGCTGGCC 
TGCATGCCTG GCAAGAAGTG CGCCCCCGAG TCAGAGCCGG GCAACAGCAC AGAGGGGGAG 
ACGCCCTGTG GCAGCAGCTT CGCCGTCTTC TACTTCATCA GCTTCTACAT GCTCTGCGCC 
TTCCTGATCA TCAACCTCTT CGTAGCCGTC ATCATGGACA ACTTTGACTA CCTGACGAGG 
GACTGGTCCA TCCTGGGTCC CCACCACCTG GACGAATTTA AGAGAATTTG GGCAGAGTAT 
GACCCTGAAG CCAAGGGACG CATCAAACAC CTGGATGTGG TGACCCTGCT CCGACGGATT 
CAGCCCCCCC TGGGTTTTGG GAAGCTGTGC CCTCACCGCG TGGCTTGCAA ACGCCTGGTC 
TCCATGAACA TGCCCCTGAA CAGCGACGGG ACTGTCATGT TCAATGCCAC GCTGTTTGCC 
CTGGTCCGTA CAGCTCTGAG GATCAAGACG GAAGGGAACC TGGAACAGGC CAATGAGGAG 
CTGCGGGCCA TCATCAAGAA GATCTGGAAA CGGACCAGCA TGAAGCTGCT GGACCAAGTG 
GTGCCGCCCG CAGGAGATGA CGAGGTCACG GTGGGCAAGT TTTACGCTAC TTTCCTGATC 
CAGGAGTATT TCCGGAAGTT CAAGAAGCGC AAAGAGCAAG GCCTGGTGGG CAAGCCCTCC 
CAGAGGAACG CCCTGTCCCT GCAGGCTGGC CTGCGCACCC TGCACGATCT GGGGCCCGAG 
ATCCGCCGGG CCATCTCTGG GGACCTGACC GCCGAGGAGG AGCTGGACAA GGCCATGAAG 
GAGGCTGTGT CTGCAGCCTC AGAAGATGAC ATCTTCAGGA GGGCCGGCGG CCTGTTCGGC 
AACCACGTGG GCTACTACCC GAGCGACGGC CGGGGCGCCT TCCCCCAGAC CTTCACCACC 
CAGCGCCCCC TGCACATCAG CAAGGCAGGC AACAGCCAGG GGGACACCGA GTCCCCCTCC 
CACGAGAAAC TGGTGGACTC CACCTTCACC CCCAGCAGCT ACTCATCCAC GGGCTCCAAC 
GCCAACATCA ACAACGCCAA CAACACCGCC CTGGGCCGCT GCCCCCGCCC CGCGCTCCAC 
TCCGGTGCTG TCAGCACCTT GGAGGCCCAC GGGCCCCCCT TGTCCCCTGC CATCCGGGTG 
CAGGAGGCCG CGTGGAGGCA CAGCTCCCAG AGGTGCCCCT CCCGGGACAG CCCGATAGCC 
ATGCTGTGTC AGGAAGAGGC CTCTGGGGAC GAGACCTGTG CCGTGCACGT GGACGAGAAC 
GCAGAGCACA GCAGCGAGCC CAGCTTGGTC TCCACGGAGA TGCTCTCCTA CCAGGATGAC 
GAGAATCGGC AGCTGACTCC CCCCGAGGAG GACAGGAGGG ACATCCGTCT GTCCCCAAAG 
AGGGGCTTCC TGCGCTCCGC CTCCCTAGGT CGAAGGGCCT CCTTCCACCT TGAATGTCTG 
AAGCGACAGA AGAATCCTGG GGGAGACGTC TCTCAGAACA CAGTCCTGCC TTTGCACCTG 
GTTCACCATC AGGCGCTGGC AGTGGCGGGT CTGAGCCCCC TCCTGCAGAG AAGCCATCCC 
CCTGGGACCC TCCCCCCACC ACGCCTCACA CCCCCCGCCA CACCTGGCCC AGCCTGGCCT 
CCGCGGCCTG TGCCCACCCT GCGGCTGGAA GGGGCCGAGT CCAGCGACAA GCTCACCAGC 
AGCTTCCCAT CCATCCACTG CGACCCCCAC ATCGGGGAGC CCACGCCCTG CGGGGTTGTC 
GGCACCCCCC GGAGGGCCCG GCCCGTGTCA CTCACTGTGC CCAGCCCGGC AGGGCCCCAG 
GGCCGTCCGT TCCATGGCAG TGCCAGCAGC CTGGTGGAGG CGGTCTTGAT CTCGGAAGGA 
TTGGGGCAGT TTGCTCAGGA CCCCAGGTTC CTCGAGGCCA CCACGCAGGA GCTGGCCGAT 
GCCTGCGACA TGACCATTGA GGAGATGGAG AGCGCGGCTG ACGACATCCT CAGCGGGGGC 
GCCGGGCAGA GCCCCAACGG CACCCTGCTC CCGTGTGCAA ACTGCAGGGA CCCGGGGCCG 
GACCGAGCGG GGGGTGTGGA GGACGCAGCC TGGGCGCCCA GCGCCGAGCC CCGCCAGGGC 
GCAGAGGAGC CCCGGGACAG CAGGGCCTTC GCCAGCGGCC TGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992763.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848531.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X16, mRNA.

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CloneID	OBa234948
Clone ID Related Accession (Same CDS sequence)	XM_024992763.1
Accession Version	XM_024992763.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCAATG AAAACACGAG GATGTACATT CCGGAGGAGA ACCACCAAGG TCCCAGCTAT 
GGGAGCCCGC GCCCGGCTCA CGCCAACATG AACGCCAACG CGGCCGCGGG GCTGGCACCT 
GAGCACATCC CCACACCGGG GGCCGCCCTG TCCTGGCAGG CCGCCATCGA CGCGGCCCGG 
CAGGCCAAGC TGATGGGCAG TGCTGGCAAC GCAACCATCT CCACGGTCAG CTCCACGCAG 
CGGAAACGGC AGCAGTATGG GAAGCCCAAG AAGCAGGGCA GCACGACAGC CACCCGCCCG 
CCCCGCGCCC TGCTCTGCCT GACCCTAAAG AACCCCATCC GGAGGGCCTG CATCAGCATC 
GTCGAGTGGA AGCCATTTGA AATCATTATT TTACTGACTA TTTTTGCCAA TTGTGTGGCC 
TTAGCGATCT ATATTCCCTT CCCAGAGGAC GACTCCAACG CCACGAATTC CAACCTGGAG 
CGGGTGGAGT ATCTCTTCCT CATCATTTTT ACTGTGGAAG CATTTTTAAA AGTCATAGCC 
TACGGACTCC TCTTTCACCC CAACGCTTAC CTCCGCAATG GCTGGAACTT ACTAGACTTT 
ATCATCGTGG TTGTCGGGCT TTTCAGCGCG ATCTTAGAGC AAGCGACCAA AGCCGATGGG 
GCCAACGCCC TGGGAGGGAA GGGTGCTGGA TTCGACGTAA AGGCGCTGAG GGCGTTCCGT 
GTGCTACGGC CCCTGCGGTT GGTGTCCGGG GTCCCGAGTC TCCAGGTGGT CTTGAATTCC 
ATCATCAAGG CCATGGTGCC CCTGCTGCAC ATCGCCCTGC TCGTGCTGTT CGTCATCATC 
ATCTATGCCA TCATCGGCCT GGAGCTCTTC ATGGGCAAGA TGCATAAGAC CTGCTACAAC 
CAGGAGGGCA TTGCAGATGT CCCAGCAGAA GACGACCCCT CGCCCTGTGC CCTGGAGACA 
GGCCACGGGC GGCAGTGCCA GAATGGCACG GTGTGCAAAC CAGGCTGGGA TGGACCCAAG 
CACGGCATCA CCAATTTCGA CAACTTCGCC TTCGCCATGC TGACTGTGTT CCAGTGCATC 
ACCATGGAGG GCTGGACCGA CGTCCTCTAC TGGGTCAATG ATGCCGTGGG AAGGGACTGG 
CCCTGGATCT ATTTTGTCAC ATTAATCATC ATAGGTTCAT TCTTTGTTCT TAACTTGGTT 
CTCGGTGTCC TTAGCGGAGA GTTCTCCAAA GAGCGAGAGA AGGCCAAGGC CCGGGGAGAC 
TTCCAGAAGC TGCGAGAGAA GCAGCAGCTG GAGGAGGACC TGAAGGGCTA CCTGGACTGG 
ATCACGCAGG CCGAGGACAT CGACCCCGAG AATGAGGACG AGGGCCTGGA CGAGGAGAAA 
CCCCGAAACA TGAGCATGCC CACCAGTGAG ACCGAGTCTG TCAACACCGA AAACGTGGCT 
GGAGGGGACA TCGAGGGAGA GAGCTGCGGG GCCAGGCTGG CCCACCGGAT CTCCAAGTCA 
AAGTTCAGCC GCTACTGGCG CCGGTGGAAT CGGTTCTGCA GGAGGAAGTG CCGCGCGGCC 
GTGAAGTCCA GCATCTTCTA CTGGCTGGTC ATCTTCCTGG TTTTCCTCAA CACACTCACC 
ATCGCCTCCG AGCACTACAA CCAGCCCCAC TGGCTCACGG AAGTCCAAGA CACGGCCAAC 
AAGGCGCTGC TGGCCCTGTT CACGGCGGAG ATGCTGCTCA AGATGTACAG CCTGGGCCTG 
CAGGCCTACT TCGTGTCGCT CTTCAACCGC TTCGACTGCT TCATCGTGTG CGGGGGCATC 
CTGGAGACCA TCCTGGTGGA GACCAAGATC ATGTCGCCGT TGGGCATCTC CGTGCTGCGT 
TGCGTGCGCC TCCTTCGGAT ATTTAAGATC ACAAGGTACT GGAACTCCCT GAGCAACCTG 
GTGGCATCCC TGCTGAACTC AGTGCGCTCC ATTGCCTCGC TGCTGCTGCT GCTCTTCCTC 
TTCATCATCA TCTTCTCCCT CCTGGGGATG CAGCTCTTTG GGGGGAAGTT CAACTTTGAT 
GAGATGCAGA CCCGGAGGAG CACCTTTGAC AACTTCCCGC AGTCCCTCCT CACCGTGTTT 
CAGATCCTGA CCGGGGAGGA CTGGAATTCG GTGATGTATG ATGGGATCAT GGCTTATGGC 
GGCCCCTCTT TTCCAGGGAT GTTAGTCTGT ATTTACTTCA TCATCCTCTT CATCTGTGGA 
AATTATATCC TACTGAACGT GTTCTTGGCC ATTGCTGTGG ACAACCTGGC TGACGCGGAG 
AGCCTCACAT CTGCCCAGAA GGAGGAGGAA GAGGAGAAGG AGAGGAAGAA GCTAGCCAGG 
ACCGCCAGCC CAGAGAAGAA GCAAGAGGTG GTAGAGAAGC CGGCCGTGGA GGAGACCAAG 
GAGGAGAAAA TCGAGCTGAA ATCCATCACG GCAGATGGAG AGTCCCCACC CGCCACCAAG 
ATCAACATGG ATGACCTCCA GCCCAACGAG AATGAGGACA AGAGCTCCTA CCCCAACCAG 
GAAACCACAG GAGAAGAGGA CGAGGAGGAG CCCGAGATGC CCGTGGGCCC CCGCCCGCGC 
CCCCTCTCTG AGCTGCACCT GAAGGAGAAG GCAGTGCCCA TGCCGGAAGC CAGCGCCTTC 
TTCATCTTCA GCCCCAGCAA CAGGTTCCGC CTCCAGTGCC ATCGCATCGT CAATGACTCC 
ATCTTCACCA ACCTGATCCT CTTCTTCATC CTGCTCAGCA GCATCTCCCT GGCCGCCGAG 
GACCCCGTGC AGCACACCTC CTTCAGGAAC CACATTCTGT TTTATTTTGA CATTGTTTTC 
ACTACCATTT TCACCATTGA GATTGCCCTG AAGATGACGG CCTACGGGGC CTTCCTGCAC 
AAGGGCTCCT TCTGCCGCAA CTACTTCAAC ATCCTGGACC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TCGGCATCCA GTCCAGTGCC ATCAACGTGG TGAAGATCCT GCGAGTCCTG 
AGGGTGCTCC GGCCACTGAG GGCCATCAAC AGGGCCAAGG GGCTGAAGCA CGTCGTGCAG 
TGCGTGTTCG TGGCCATCCG CACCATCGGG AACATTGTGA TCGTCACCAC GCTGCTCCAG 
TTCATGTTCG CCTGCATTGG GGTCCAGCTC TTCAAGGGAA AGCTCTACAC CTGTTCGGAC 
AGTTCCAAAC AGACAGAGGC TGAATGCAAG GGGAACTACA TAACATACAA GGATGGGGAG 
GTGGACCACC CCATCATCCA GCCCCGCAGC TGGGAGAACA GCAAGTTTGA CTTTGACAAT 
GTCCTGGCAG CCATGATGGC CCTGTTCACC GTCTCCACCT TCGAGGGATG GCCAGAGTTG 
CTGTACCGCT CCATCGACTC TCACACGGAG GACAAGGGTC CCATATACAA CTACCGCGTG 
GAGATCTCCG TCTTCTTCAT CATCTACATC ATCATCATTG CCTTCTTCAT GATGAACATC 
TTCGTGGGTT TCGTCATCGT CACCTTCCAG GAGCAGGGGG AGCAGGAATA CAAGAACTGC 
GAGCTGGACA AGAACCAGCG GCAGTGTGTG GAGTATGCCC TCAAGGCCCG GCCCCTGCGG 
AGGTACATCC CGAAAAACCA GCACCAGTAC AAGGTGTGGT ACGTGGTCAA CTCCACCTAC 
TTCGAGTACC TGATGTTCGT TCTCATTCTG CTCAACACCA TCTGCCTGGC CATGCAGCAC 
TACGGTCAGA GCTGTCTGTT CAAAATCGCC ATGAACATCC TCAACATGCT CTTCACCGGC 
CTCTTCACAG TGGAGATGGT CCTGAAGCTC ATCGCCTTCA AGCCCAAGCA CTATTTCTGT 
GATGCATGGA ACACATTTGA CGCCTTGATT GTTGTGGGTA GCATTGTTGA TATAGCCATC 
ACCGAGGTAA ACCCAGCTGA ACATACCCAA TGCTCTCCCT TTATGAACGC GGAAGAGAAC 
TCTCGCATCT CCATCACCTT CTTCCGCCTC TTCCGGGTCA TGCGTCTGGT CAAGCTGCTG 
AGCCGCGGGG AGGGCATCCG GACACTGCTG TGGACCTTCA TCAAGTCCTT CCAGGCCCTA 
CCCTATGTGG CCCTCCTGAT CGTGATGCTG TTTTTCATCT ACGCCGTGAT CGGGATGCAG 
GTGTTTGGCA AAATTGCCCT GAATGACACG ACAGAGATCA ACAGGAACAA CAACTTCCAG 
ACCTTCCCCC AGGCCGTGCT GCTCCTCTTC AGGTGCGCCA CTGGGGAGGC CTGGCAGGAC 
ATCATGCTGG CCTGCATGCC TGGCAAGAAG TGCGCCCCCG AGTCAGAGCC GGGCAACAGC 
ACAGAGGGGG AGACGCCCTG TGGCAGCAGC TTCGCCGTCT TCTACTTCAT CAGCTTCTAC 
ATGCTCTGCG CCTTCCTGAT CATCAACCTC TTCGTAGCCG TCATCATGGA CAACTTTGAC 
TACCTGACGA GGGACTGGTC CATCCTGGGT CCCCACCACC TGGACGAATT TAAGAGAATT 
TGGGCAGAGT ATGACCCTGA AGCCAAGGGA CGCATCAAAC ACCTGGATGT GGTGACCCTG 
CTCCGACGGA TTCAGCCCCC CCTGGGTTTT GGGAAGCTGT GCCCTCACCG CGTGGCTTGC 
AAACGCCTGG TCTCCATGAA CATGCCCCTG AACAGCGACG GGACTGTCAT GTTCAATGCC 
ACGCTGTTTG CCCTGGTCCG TACAGCTCTG AGGATCAAGA CGGAAGGGAA CCTGGAACAG 
GCCAATGAGG AGCTGCGGGC CATCATCAAG AAGATCTGGA AACGGACCAG CATGAAGCTG 
CTGGACCAAG TGGTGCCGCC CGCAGGAGAT GACGAGGTCA CGGTGGGCAA GTTTTACGCT 
ACTTTCCTGA TCCAGGAGTA TTTCCGGAAG TTCAAGAAGC GCAAAGAGCA AGGCCTGGTG 
GGCAAGCCCT CCCAGAGGAA CGCCCTGTCC CTGCAGGCTG GCCTGCGCAC CCTGCACGAT 
CTGGGGCCCG AGATCCGCCG GGCCATCTCT GGGGACCTGA CCGCCGAGGA GGAGCTGGAC 
AAGGCCATGA AGGAGGCTGT GTCTGCAGCC TCAGAAGATG ACATCTTCAG GAGGGCCGGC 
GGCCTGTTCG GCAACCACGT GGGCTACTAC CCGAGCGACG GCCGGGGCGC CTTCCCCCAG 
ACCTTCACCA CCCAGCGCCC CCTGCACATC AGCAAGGCAG GCAACAGCCA GGGGGACACC 
GAGTCCCCCT CCCACGAGAA ACTGGTGGAC TCCACCTTCA CCCCCAGCAG CTACTCATCC 
ACGGGCTCCA ACGCCAACAT CAACAACGCC AACAACACCG CCCTGGGCCG CTGCCCCCGC 
CCCGCGCTCC ACTCCGGTGC TGTCAGCACC TTGGAGGCCC ACGGGCCCCC CTTGTCCCCT 
GCCATCCGGG TGCAGGAGGC CGCGTGGAGG CACAGCTCCC AGAGGTGCCC CTCCCGGGAC 
AGCCCGATAG CCATGCTGTG TCAGGAAGAG GCCTCTGGGG ACGAGACCTG TGCCGTGCAC 
GTGGACGAGA ACGCAGAGCA CAGCAGCGAG CCCAGCTTGG TCTCCACGGA GATGCTCTCC 
TACCAGGATG ACGAGAATCG GCAGCTGACT CCCCCCGAGG AGGACAGGAG GGACATCCGT 
CTGTCCCCAA AGAGGGGCTT CCTGCGCTCC GCCTCCCTAG GTCGAAGGGC CTCCTTCCAC 
CTTGAATGTC TGAAGCGACA GAAGAATCCT GGGGGAGACG TCTCTCAGAA CACAGTCCTG 
CCTTTGCACC TGGTTCACCA TCAGGCGCTG GCAGTGGCGG GTCTGAGCCC CCTCCTGCAG 
AGAAGCCATC CCCCTGGGAC CCTCCCCCCA CCACGCCTCA CACCCCCCGC CACACCTGGC 
CCAGCCTGGC CTCCGCGGCC TGTGCCCACC CTGCGGCTGG AAGGGGCCGA GTCCAGCGAC 
AAGCTCACCA GCAGCTTCCC ATCCATCCAC TGCGACCCCC ACATCGGGGA GCCCACGCCC 
TGCGGGGTTG TCGGCACCCC CCGGAGGGCC CGGCCCGTGT CACTCACTGT GCCCAGCCCG 
GCAGGGCCCC AGGGCCGTCC GTTCCATGGC AGTGCCAGCA GCCTGGTGGA GGCGGTCTTG 
ATCTCGGAAG GATTGGGGCA GTTTGCTCAG GACCCCAGGT TCCTCGAGGC CACCACGCAG 
GAGCTGGCCG ATGCCTGCGA CATGACCATT GAGGAGATGG AGAGCGCGGC TGACGACATC 
CTCAGCGGGG GCGCCGGGCA GAGCCCCAAC GGCACCCTGC TCCCGTGTGC AAACTGCAGG 
GACCCGGGGC CGGACCGAGC GGGGGGTGTG GAGGACGCAG CCTGGGCGCC CAGCGCCGAG 
CCCCGCCAGG GCGCAGAGGA GCCCCGGGAC AGCAGGGCCT TCGCCAGCGG CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848531.1
CDS	556..6972
Translation	MVNENTRMYIPEENHQGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992763.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992763.1

ORF Insert Sequence:

ATGGTCAATG AAAACACGAG GATGTACATT CCGGAGGAGA ACCACCAAGG TCCCAGCTAT 
GGGAGCCCGC GCCCGGCTCA CGCCAACATG AACGCCAACG CGGCCGCGGG GCTGGCACCT 
GAGCACATCC CCACACCGGG GGCCGCCCTG TCCTGGCAGG CCGCCATCGA CGCGGCCCGG 
CAGGCCAAGC TGATGGGCAG TGCTGGCAAC GCAACCATCT CCACGGTCAG CTCCACGCAG 
CGGAAACGGC AGCAGTATGG GAAGCCCAAG AAGCAGGGCA GCACGACAGC CACCCGCCCG 
CCCCGCGCCC TGCTCTGCCT GACCCTAAAG AACCCCATCC GGAGGGCCTG CATCAGCATC 
GTCGAGTGGA AGCCATTTGA AATCATTATT TTACTGACTA TTTTTGCCAA TTGTGTGGCC 
TTAGCGATCT ATATTCCCTT CCCAGAGGAC GACTCCAACG CCACGAATTC CAACCTGGAG 
CGGGTGGAGT ATCTCTTCCT CATCATTTTT ACTGTGGAAG CATTTTTAAA AGTCATAGCC 
TACGGACTCC TCTTTCACCC CAACGCTTAC CTCCGCAATG GCTGGAACTT ACTAGACTTT 
ATCATCGTGG TTGTCGGGCT TTTCAGCGCG ATCTTAGAGC AAGCGACCAA AGCCGATGGG 
GCCAACGCCC TGGGAGGGAA GGGTGCTGGA TTCGACGTAA AGGCGCTGAG GGCGTTCCGT 
GTGCTACGGC CCCTGCGGTT GGTGTCCGGG GTCCCGAGTC TCCAGGTGGT CTTGAATTCC 
ATCATCAAGG CCATGGTGCC CCTGCTGCAC ATCGCCCTGC TCGTGCTGTT CGTCATCATC 
ATCTATGCCA TCATCGGCCT GGAGCTCTTC ATGGGCAAGA TGCATAAGAC CTGCTACAAC 
CAGGAGGGCA TTGCAGATGT CCCAGCAGAA GACGACCCCT CGCCCTGTGC CCTGGAGACA 
GGCCACGGGC GGCAGTGCCA GAATGGCACG GTGTGCAAAC CAGGCTGGGA TGGACCCAAG 
CACGGCATCA CCAATTTCGA CAACTTCGCC TTCGCCATGC TGACTGTGTT CCAGTGCATC 
ACCATGGAGG GCTGGACCGA CGTCCTCTAC TGGGTCAATG ATGCCGTGGG AAGGGACTGG 
CCCTGGATCT ATTTTGTCAC ATTAATCATC ATAGGTTCAT TCTTTGTTCT TAACTTGGTT 
CTCGGTGTCC TTAGCGGAGA GTTCTCCAAA GAGCGAGAGA AGGCCAAGGC CCGGGGAGAC 
TTCCAGAAGC TGCGAGAGAA GCAGCAGCTG GAGGAGGACC TGAAGGGCTA CCTGGACTGG 
ATCACGCAGG CCGAGGACAT CGACCCCGAG AATGAGGACG AGGGCCTGGA CGAGGAGAAA 
CCCCGAAACA TGAGCATGCC CACCAGTGAG ACCGAGTCTG TCAACACCGA AAACGTGGCT 
GGAGGGGACA TCGAGGGAGA GAGCTGCGGG GCCAGGCTGG CCCACCGGAT CTCCAAGTCA 
AAGTTCAGCC GCTACTGGCG CCGGTGGAAT CGGTTCTGCA GGAGGAAGTG CCGCGCGGCC 
GTGAAGTCCA GCATCTTCTA CTGGCTGGTC ATCTTCCTGG TTTTCCTCAA CACACTCACC 
ATCGCCTCCG AGCACTACAA CCAGCCCCAC TGGCTCACGG AAGTCCAAGA CACGGCCAAC 
AAGGCGCTGC TGGCCCTGTT CACGGCGGAG ATGCTGCTCA AGATGTACAG CCTGGGCCTG 
CAGGCCTACT TCGTGTCGCT CTTCAACCGC TTCGACTGCT TCATCGTGTG CGGGGGCATC 
CTGGAGACCA TCCTGGTGGA GACCAAGATC ATGTCGCCGT TGGGCATCTC CGTGCTGCGT 
TGCGTGCGCC TCCTTCGGAT ATTTAAGATC ACAAGGTACT GGAACTCCCT GAGCAACCTG 
GTGGCATCCC TGCTGAACTC AGTGCGCTCC ATTGCCTCGC TGCTGCTGCT GCTCTTCCTC 
TTCATCATCA TCTTCTCCCT CCTGGGGATG CAGCTCTTTG GGGGGAAGTT CAACTTTGAT 
GAGATGCAGA CCCGGAGGAG CACCTTTGAC AACTTCCCGC AGTCCCTCCT CACCGTGTTT 
CAGATCCTGA CCGGGGAGGA CTGGAATTCG GTGATGTATG ATGGGATCAT GGCTTATGGC 
GGCCCCTCTT TTCCAGGGAT GTTAGTCTGT ATTTACTTCA TCATCCTCTT CATCTGTGGA 
AATTATATCC TACTGAACGT GTTCTTGGCC ATTGCTGTGG ACAACCTGGC TGACGCGGAG 
AGCCTCACAT CTGCCCAGAA GGAGGAGGAA GAGGAGAAGG AGAGGAAGAA GCTAGCCAGG 
ACCGCCAGCC CAGAGAAGAA GCAAGAGGTG GTAGAGAAGC CGGCCGTGGA GGAGACCAAG 
GAGGAGAAAA TCGAGCTGAA ATCCATCACG GCAGATGGAG AGTCCCCACC CGCCACCAAG 
ATCAACATGG ATGACCTCCA GCCCAACGAG AATGAGGACA AGAGCTCCTA CCCCAACCAG 
GAAACCACAG GAGAAGAGGA CGAGGAGGAG CCCGAGATGC CCGTGGGCCC CCGCCCGCGC 
CCCCTCTCTG AGCTGCACCT GAAGGAGAAG GCAGTGCCCA TGCCGGAAGC CAGCGCCTTC 
TTCATCTTCA GCCCCAGCAA CAGGTTCCGC CTCCAGTGCC ATCGCATCGT CAATGACTCC 
ATCTTCACCA ACCTGATCCT CTTCTTCATC CTGCTCAGCA GCATCTCCCT GGCCGCCGAG 
GACCCCGTGC AGCACACCTC CTTCAGGAAC CACATTCTGT TTTATTTTGA CATTGTTTTC 
ACTACCATTT TCACCATTGA GATTGCCCTG AAGATGACGG CCTACGGGGC CTTCCTGCAC 
AAGGGCTCCT TCTGCCGCAA CTACTTCAAC ATCCTGGACC TGCTGGTGGT CAGTGTGTCC 
CTCATCTCCT TCGGCATCCA GTCCAGTGCC ATCAACGTGG TGAAGATCCT GCGAGTCCTG 
AGGGTGCTCC GGCCACTGAG GGCCATCAAC AGGGCCAAGG GGCTGAAGCA CGTCGTGCAG 
TGCGTGTTCG TGGCCATCCG CACCATCGGG AACATTGTGA TCGTCACCAC GCTGCTCCAG 
TTCATGTTCG CCTGCATTGG GGTCCAGCTC TTCAAGGGAA AGCTCTACAC CTGTTCGGAC 
AGTTCCAAAC AGACAGAGGC TGAATGCAAG GGGAACTACA TAACATACAA GGATGGGGAG 
GTGGACCACC CCATCATCCA GCCCCGCAGC TGGGAGAACA GCAAGTTTGA CTTTGACAAT 
GTCCTGGCAG CCATGATGGC CCTGTTCACC GTCTCCACCT TCGAGGGATG GCCAGAGTTG 
CTGTACCGCT CCATCGACTC TCACACGGAG GACAAGGGTC CCATATACAA CTACCGCGTG 
GAGATCTCCG TCTTCTTCAT CATCTACATC ATCATCATTG CCTTCTTCAT GATGAACATC 
TTCGTGGGTT TCGTCATCGT CACCTTCCAG GAGCAGGGGG AGCAGGAATA CAAGAACTGC 
GAGCTGGACA AGAACCAGCG GCAGTGTGTG GAGTATGCCC TCAAGGCCCG GCCCCTGCGG 
AGGTACATCC CGAAAAACCA GCACCAGTAC AAGGTGTGGT ACGTGGTCAA CTCCACCTAC 
TTCGAGTACC TGATGTTCGT TCTCATTCTG CTCAACACCA TCTGCCTGGC CATGCAGCAC 
TACGGTCAGA GCTGTCTGTT CAAAATCGCC ATGAACATCC TCAACATGCT CTTCACCGGC 
CTCTTCACAG TGGAGATGGT CCTGAAGCTC ATCGCCTTCA AGCCCAAGCA CTATTTCTGT 
GATGCATGGA ACACATTTGA CGCCTTGATT GTTGTGGGTA GCATTGTTGA TATAGCCATC 
ACCGAGGTAA ACCCAGCTGA ACATACCCAA TGCTCTCCCT TTATGAACGC GGAAGAGAAC 
TCTCGCATCT CCATCACCTT CTTCCGCCTC TTCCGGGTCA TGCGTCTGGT CAAGCTGCTG 
AGCCGCGGGG AGGGCATCCG GACACTGCTG TGGACCTTCA TCAAGTCCTT CCAGGCCCTA 
CCCTATGTGG CCCTCCTGAT CGTGATGCTG TTTTTCATCT ACGCCGTGAT CGGGATGCAG 
GTGTTTGGCA AAATTGCCCT GAATGACACG ACAGAGATCA ACAGGAACAA CAACTTCCAG 
ACCTTCCCCC AGGCCGTGCT GCTCCTCTTC AGGTGCGCCA CTGGGGAGGC CTGGCAGGAC 
ATCATGCTGG CCTGCATGCC TGGCAAGAAG TGCGCCCCCG AGTCAGAGCC GGGCAACAGC 
ACAGAGGGGG AGACGCCCTG TGGCAGCAGC TTCGCCGTCT TCTACTTCAT CAGCTTCTAC 
ATGCTCTGCG CCTTCCTGAT CATCAACCTC TTCGTAGCCG TCATCATGGA CAACTTTGAC 
TACCTGACGA GGGACTGGTC CATCCTGGGT CCCCACCACC TGGACGAATT TAAGAGAATT 
TGGGCAGAGT ATGACCCTGA AGCCAAGGGA CGCATCAAAC ACCTGGATGT GGTGACCCTG 
CTCCGACGGA TTCAGCCCCC CCTGGGTTTT GGGAAGCTGT GCCCTCACCG CGTGGCTTGC 
AAACGCCTGG TCTCCATGAA CATGCCCCTG AACAGCGACG GGACTGTCAT GTTCAATGCC 
ACGCTGTTTG CCCTGGTCCG TACAGCTCTG AGGATCAAGA CGGAAGGGAA CCTGGAACAG 
GCCAATGAGG AGCTGCGGGC CATCATCAAG AAGATCTGGA AACGGACCAG CATGAAGCTG 
CTGGACCAAG TGGTGCCGCC CGCAGGAGAT GACGAGGTCA CGGTGGGCAA GTTTTACGCT 
ACTTTCCTGA TCCAGGAGTA TTTCCGGAAG TTCAAGAAGC GCAAAGAGCA AGGCCTGGTG 
GGCAAGCCCT CCCAGAGGAA CGCCCTGTCC CTGCAGGCTG GCCTGCGCAC CCTGCACGAT 
CTGGGGCCCG AGATCCGCCG GGCCATCTCT GGGGACCTGA CCGCCGAGGA GGAGCTGGAC 
AAGGCCATGA AGGAGGCTGT GTCTGCAGCC TCAGAAGATG ACATCTTCAG GAGGGCCGGC 
GGCCTGTTCG GCAACCACGT GGGCTACTAC CCGAGCGACG GCCGGGGCGC CTTCCCCCAG 
ACCTTCACCA CCCAGCGCCC CCTGCACATC AGCAAGGCAG GCAACAGCCA GGGGGACACC 
GAGTCCCCCT CCCACGAGAA ACTGGTGGAC TCCACCTTCA CCCCCAGCAG CTACTCATCC 
ACGGGCTCCA ACGCCAACAT CAACAACGCC AACAACACCG CCCTGGGCCG CTGCCCCCGC 
CCCGCGCTCC ACTCCGGTGC TGTCAGCACC TTGGAGGCCC ACGGGCCCCC CTTGTCCCCT 
GCCATCCGGG TGCAGGAGGC CGCGTGGAGG CACAGCTCCC AGAGGTGCCC CTCCCGGGAC 
AGCCCGATAG CCATGCTGTG TCAGGAAGAG GCCTCTGGGG ACGAGACCTG TGCCGTGCAC 
GTGGACGAGA ACGCAGAGCA CAGCAGCGAG CCCAGCTTGG TCTCCACGGA GATGCTCTCC 
TACCAGGATG ACGAGAATCG GCAGCTGACT CCCCCCGAGG AGGACAGGAG GGACATCCGT 
CTGTCCCCAA AGAGGGGCTT CCTGCGCTCC GCCTCCCTAG GTCGAAGGGC CTCCTTCCAC 
CTTGAATGTC TGAAGCGACA GAAGAATCCT GGGGGAGACG TCTCTCAGAA CACAGTCCTG 
CCTTTGCACC TGGTTCACCA TCAGGCGCTG GCAGTGGCGG GTCTGAGCCC CCTCCTGCAG 
AGAAGCCATC CCCCTGGGAC CCTCCCCCCA CCACGCCTCA CACCCCCCGC CACACCTGGC 
CCAGCCTGGC CTCCGCGGCC TGTGCCCACC CTGCGGCTGG AAGGGGCCGA GTCCAGCGAC 
AAGCTCACCA GCAGCTTCCC ATCCATCCAC TGCGACCCCC ACATCGGGGA GCCCACGCCC 
TGCGGGGTTG TCGGCACCCC CCGGAGGGCC CGGCCCGTGT CACTCACTGT GCCCAGCCCG 
GCAGGGCCCC AGGGCCGTCC GTTCCATGGC AGTGCCAGCA GCCTGGTGGA GGCGGTCTTG 
ATCTCGGAAG GATTGGGGCA GTTTGCTCAG GACCCCAGGT TCCTCGAGGC CACCACGCAG 
GAGCTGGCCG ATGCCTGCGA CATGACCATT GAGGAGATGG AGAGCGCGGC TGACGACATC 
CTCAGCGGGG GCGCCGGGCA GAGCCCCAAC GGCACCCTGC TCCCGTGTGC AAACTGCAGG 
GACCCGGGGC CGGACCGAGC GGGGGGTGTG GAGGACGCAG CCTGGGCGCC CAGCGCCGAG 
CCCCGCCAGG GCGCAGAGGA GCCCCGGGAC AGCAGGGCCT TCGCCAGCGG CCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA1C ( XM_024992764.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848532.1 Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X17, mRNA.

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CloneID	OBa234949
Clone ID Related Accession (Same CDS sequence)	XM_024992764.1
Accession Version	XM_024992764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	voltage-dependent L-type calcium channel subunit alpha-1C isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC ACTATTTCTG TGATGCATGG 
AACACATTTG ACGCCTTGAT TGTTGTGGGT AGCATTGTTG ATATAGCCAT CACCGAGGTA 
AACCCAGCTG AACATACCCA ATGCTCTCCC TTTATGAACG CGGAAGAGAA CTCTCGCATC 
TCCATCACCT TCTTCCGCCT CTTCCGGGTC ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG 
GAGGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TCCAGGCCCT ACCCTATGTG 
GCCCTCCTGA TCGTGATGCT GTTTTTCATC TACGCCGTGA TCGGGATGCA GGTGTTTGGC 
AAAATTGCCC TGAATGACAC GACAGAGATC AACAGGAACA ACAACTTCCA GACCTTCCCC 
CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC ACTGGGGAGG CCTGGCAGGA CATCATGCTG 
GCCTGCATGC CTGGCAAGAA GTGCGCCCCC GAGTCAGAGC CGGGCAACAG CACAGAGGGG 
GAGACGCCCT GTGGCAGCAG CTTCGCCGTC TTCTACTTCA TCAGCTTCTA CATGCTCTGC 
GCCTTCCTGA TCATCAACCT CTTCGTAGCC GTCATCATGG ACAACTTTGA CTACCTGACG 
AGGGACTGGT CCATCCTGGG TCCCCACCAC CTGGACGAAT TTAAGAGAAT TTGGGCAGAG 
TATGACCCTG AAGCCAAGGG ACGCATCAAA CACCTGGATG TGGTGACCCT GCTCCGACGG 
ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG TGCCCTCACC GCGTGGCTTG CAAACGCCTG 
GTCTCCATGA ACATGCCCCT GAACAGCGAC GGGACTGTCA TGTTCAATGC CACGCTGTTT 
GCCCTGGTCC GTACAGCTCT GAGGATCAAG ACGGAAGGGA ACCTGGAACA GGCCAATGAG 
GAGCTGCGGG CCATCATCAA GAAGATCTGG AAACGGACCA GCATGAAGCT GCTGGACCAA 
GTGGTGCCGC CCGCAGGAGA TGACGAGGTC ACGGTGGGCA AGTTTTACGC TACTTTCCTG 
ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC 
TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT GGCCTGCGCA CCCTGCACGA TCTGGGGCCC 
GAGATCCGCC GGGCCATCTC TGGGGACCTG ACCGCCGAGG AGGAGCTGGA CAAGGCCATG 
AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT GACATCTTCA GGAGGGCCGG CGGCCTGTTC 
GGCAACCACG TGGGCTACTA CCCGAGCGAC GGCCGGGGCG CCTTCCCCCA GACCTTCACC 
ACCCAGCGCC CCCTGCACAT CAGCAAGGCA GGCAACAGCC AGGGGGACAC CGAGTCCCCC 
TCCCACGAGA AACTGGTGGA CTCCACCTTC ACCCCCAGCA GCTACTCATC CACGGGCTCC 
AACGCCAACA TCAACAACGC CAACAACACC GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC 
CACTCCGGTG CTGTCAGCAC CTTGGAGGCC CACGGGCCCC CCTTGTCCCC TGCCATCCGG 
GTGCAGGAGG CCGCGTGGAG GCACAGCTCC CAGAGGTGCC CCTCCCGGGA CAGCCCGATA 
GCCATGCTGT GTCAGGAAGA GGCCTCTGGG GACGAGACCT GTGCCGTGCA CGTGGACGAG 
AACGCAGAGC ACAGCAGCGA GCCCAGCTTG GTCTCCACGG AGATGCTCTC CTACCAGGAT 
GACGAGAATC GGCAGCTGAC TCCCCCCGAG GAGGACAGGA GGGACATCCG TCTGTCCCCA 
AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA GGTCGAAGGG CCTCCTTCCA CCTTGAATGT 
CTGAAGCGAC AGAAGAATCC TGGGGGAGAC GTCTCTCAGA ACACAGTCCT GCCTTTGCAC 
CTGGTTCACC ATCAGGCGCT GGCAGTGGCG GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT 
CCCCCTGGGA CCCTCCCCCC ACCACGCCTC ACACCCCCCG CCACACCTGG CCCAGCCTGG 
CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG GAAGGGGCCG AGTCCAGCGA CAAGCTCACC 
AGCAGCTTCC CATCCATCCA CTGCGACCCC CACATCGGGG AGCCCACGCC CTGCGGGGTT 
GTCGGCACCC CCCGGAGGGC CCGGCCCGTG TCACTCACTG TGCCCAGCCC GGCAGGGCCC 
CAGGGCCGTC CGTTCCATGG CAGTGCCAGC AGCCTGGTGG AGGCGGTCTT GATCTCGGAA 
GGATTGGGGC AGTTTGCTCA GGACCCCAGG TTCCTCGAGG CCACCACGCA GGAGCTGGCC 
GATGCCTGCG ACATGACCAT TGAGGAGATG GAGAGCGCGG CTGACGACAT CCTCAGCGGG 
GGCGCCGGGC AGAGCCCCAA CGGCACCCTG CTCCCGTGTG CAAACTGCAG GGACCCGGGG 
CCGGACCGAG CGGGGGGTGT GGAGGACGCA GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG 
GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848532.1
CDS	1839..8486
Translation	MLRAFFPHSTPGYQPLPRRPAKTQAGSRGTLVHEAQLSCSYVSPGGPSYGSPRPAHANMNANAAAGLAPEHIPTPGAALSWQAAIDAARQAKLMGSAGNATISTVSSTQRKRQQYGKPKKQGSTTATRPPRALLCLTLKNPIRRACISIVEWKPFEIIILLTIFANCVALAIYIPFPEDDSNATNSNLERVEYLFLIIFTVEAFLKVIAYGLLFHPNAYLRNGWNLLDFIIVVVGLFSAILEQATKADGANALGGKGAGFDVKALRAFRVLRPLRLVSGVPSLQVVLNSIIKAMVPLLHIALLVLFVIIIYAIIGLELFMGKMHKTCYNQEGIADVPAEDDPSPCALETGHGRQCQNGTVCKPGWDGPKHGITNFDNFAFAMLTVFQCITMEGWTDVLYWVNDAVGRDWPWIYFVTLIIIGSFFVLNLVLGVLSGEFSKEREKAKARGDFQKLREKQQLEEDLKGYLDWITQAEDIDPENEDEGLDEEKPRNMSMPTSETESVNTENVAGGDIEGESCGARLAHRISKSKFSRYWRRWNRFCRRKCRAAVKSSIFYWLVIFLVFLNTLTIASEHYNQPHWLTEVQDTANKALLALFTAEMLLKMYSLGLQAYFVSLFNRFDCFIVCGGILETILVETKIMSPLGISVLRCVRLLRIFKITRYWNSLSNLVASLLNSVRSIASLLLLLFLFIIIFSLLGMQLFGGKFNFDEMQTRRSTFDNFPQSLLTVFQILTGEDWNSVMYDGIMAYGGPSFPGMLVCIYFIILFICGNYILLNVFLAIAVDNLADAESLTSAQKEEEEEKERKKLARTASPEKKQEVVEKPAVEETKEEKIELKSITADGESPPATKINMDDLQPNENEDKSSYPNQETTGEEDEEEPEMPVGPRPRPLSELHLKEKAVPMPEASAFFIFSPSNRFRLQCHRIVNDSIFTNLILFFILLSSISLAAEDPVQHTSFRNHILFYFDIVFTTIFTIEIALKILGNADYVFTSIFTLEIILKMTAYGAFLHKGSFCRNYFNILDLLVVSVSLISFGIQSSAINVVKILRVLRVLRPLRAINRAKGLKHVVQCVFVAIRTIGNIVIVTTLLQFMFACIGVQLFKGKLYTCSDSSKQTEAECKGNYITYKDGEVDHPIIQPRSWENSKFDFDNVLAAMMALFTVSTFEGWPELLYRSIDSHTEDKGPIYNYRVEISVFFIIYIIIIAFFMMNIFVGFVIVTFQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPKNQHQYKVWYVVNSTYFEYLMFVLILLNTICLAMQHYGQSCLFKIAMNILNMLFTGLFTVEMVLKLIAFKPKGYFSDPWNVFDFLIVIGSVIDVILSETNHYFCDAWNTFDALIVVGSIVDIAITEVNPAEHTQCSPFMNAEENSRISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKCAPESEPGNSTEGETPCGSSFAVFYFISFYMLCAFLIINLFVAVIMDNFDYLTRDWSILGPHHLDEFKRIWAEYDPEAKGRIKHLDVVTLLRRIQPPLGFGKLCPHRVACKRLVSMNMPLNSDGTVMFNATLFALVRTALRIKTEGNLEQANEELRAIIKKIWKRTSMKLLDQVVPPAGDDEVTVGKFYATFLIQEYFRKFKKRKEQGLVGKPSQRNALSLQAGLRTLHDLGPEIRRAISGDLTAEEELDKAMKEAVSAASEDDIFRRAGGLFGNHVGYYPSDGRGAFPQTFTTQRPLHISKAGNSQGDTESPSHEKLVDSTFTPSSYSSTGSNANINNANNTALGRCPRPALHSGAVSTLEAHGPPLSPAIRVQEAAWRHSSQRCPSRDSPIAMLCQEEASGDETCAVHVDENAEHSSEPSLVSTEMLSYQDDENRQLTPPEEDRRDIRLSPKRGFLRSASLGRRASFHLECLKRQKNPGGDVSQNTVLPLHLVHHQALAVAGLSPLLQRSHPPGTLPPPRLTPPATPGPAWPPRPVPTLRLEGAESSDKLTSSFPSIHCDPHIGEPTPCGVVGTPRRARPVSLTVPSPAGPQGRPFHGSASSLVEAVLISEGLGQFAQDPRFLEATTQELADACDMTIEEMESAADDILSGGAGQSPNGTLLPCANCRDPGPDRAGGVEDAAWAPSAEPRQGAEEPRDSRAFASGL

Target ORF information:

RefSeq Version	XM_024992764.1
Organism	Bos taurus(cattle)
Definition	Bos taurus calcium voltage-gated channel subunit alpha1 C (CACNA1C), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992764.1

ORF Insert Sequence:

ATGCTGCGAG CCTTCTTTCC CCACTCCACG CCCGGGTACC AGCCGCTGCC CCGCCGCCCC 
GCCAAAACCC AGGCGGGCTC CAGGGGTACG CTGGTGCACG AGGCTCAGCT GAGCTGCTCC 
TATGTCTCTC CTGGAGGTCC CAGCTATGGG AGCCCGCGCC CGGCTCACGC CAACATGAAC 
GCCAACGCGG CCGCGGGGCT GGCACCTGAG CACATCCCCA CACCGGGGGC CGCCCTGTCC 
TGGCAGGCCG CCATCGACGC GGCCCGGCAG GCCAAGCTGA TGGGCAGTGC TGGCAACGCA 
ACCATCTCCA CGGTCAGCTC CACGCAGCGG AAACGGCAGC AGTATGGGAA GCCCAAGAAG 
CAGGGCAGCA CGACAGCCAC CCGCCCGCCC CGCGCCCTGC TCTGCCTGAC CCTAAAGAAC 
CCCATCCGGA GGGCCTGCAT CAGCATCGTC GAGTGGAAGC CATTTGAAAT CATTATTTTA 
CTGACTATTT TTGCCAATTG TGTGGCCTTA GCGATCTATA TTCCCTTCCC AGAGGACGAC 
TCCAACGCCA CGAATTCCAA CCTGGAGCGG GTGGAGTATC TCTTCCTCAT CATTTTTACT 
GTGGAAGCAT TTTTAAAAGT CATAGCCTAC GGACTCCTCT TTCACCCCAA CGCTTACCTC 
CGCAATGGCT GGAACTTACT AGACTTTATC ATCGTGGTTG TCGGGCTTTT CAGCGCGATC 
TTAGAGCAAG CGACCAAAGC CGATGGGGCC AACGCCCTGG GAGGGAAGGG TGCTGGATTC 
GACGTAAAGG CGCTGAGGGC GTTCCGTGTG CTACGGCCCC TGCGGTTGGT GTCCGGGGTC 
CCGAGTCTCC AGGTGGTCTT GAATTCCATC ATCAAGGCCA TGGTGCCCCT GCTGCACATC 
GCCCTGCTCG TGCTGTTCGT CATCATCATC TATGCCATCA TCGGCCTGGA GCTCTTCATG 
GGCAAGATGC ATAAGACCTG CTACAACCAG GAGGGCATTG CAGATGTCCC AGCAGAAGAC 
GACCCCTCGC CCTGTGCCCT GGAGACAGGC CACGGGCGGC AGTGCCAGAA TGGCACGGTG 
TGCAAACCAG GCTGGGATGG ACCCAAGCAC GGCATCACCA ATTTCGACAA CTTCGCCTTC 
GCCATGCTGA CTGTGTTCCA GTGCATCACC ATGGAGGGCT GGACCGACGT CCTCTACTGG 
GTCAATGATG CCGTGGGAAG GGACTGGCCC TGGATCTATT TTGTCACATT AATCATCATA 
GGTTCATTCT TTGTTCTTAA CTTGGTTCTC GGTGTCCTTA GCGGAGAGTT CTCCAAAGAG 
CGAGAGAAGG CCAAGGCCCG GGGAGACTTC CAGAAGCTGC GAGAGAAGCA GCAGCTGGAG 
GAGGACCTGA AGGGCTACCT GGACTGGATC ACGCAGGCCG AGGACATCGA CCCCGAGAAT 
GAGGACGAGG GCCTGGACGA GGAGAAACCC CGAAACATGA GCATGCCCAC CAGTGAGACC 
GAGTCTGTCA ACACCGAAAA CGTGGCTGGA GGGGACATCG AGGGAGAGAG CTGCGGGGCC 
AGGCTGGCCC ACCGGATCTC CAAGTCAAAG TTCAGCCGCT ACTGGCGCCG GTGGAATCGG 
TTCTGCAGGA GGAAGTGCCG CGCGGCCGTG AAGTCCAGCA TCTTCTACTG GCTGGTCATC 
TTCCTGGTTT TCCTCAACAC ACTCACCATC GCCTCCGAGC ACTACAACCA GCCCCACTGG 
CTCACGGAAG TCCAAGACAC GGCCAACAAG GCGCTGCTGG CCCTGTTCAC GGCGGAGATG 
CTGCTCAAGA TGTACAGCCT GGGCCTGCAG GCCTACTTCG TGTCGCTCTT CAACCGCTTC 
GACTGCTTCA TCGTGTGCGG GGGCATCCTG GAGACCATCC TGGTGGAGAC CAAGATCATG 
TCGCCGTTGG GCATCTCCGT GCTGCGTTGC GTGCGCCTCC TTCGGATATT TAAGATCACA 
AGGTACTGGA ACTCCCTGAG CAACCTGGTG GCATCCCTGC TGAACTCAGT GCGCTCCATT 
GCCTCGCTGC TGCTGCTGCT CTTCCTCTTC ATCATCATCT TCTCCCTCCT GGGGATGCAG 
CTCTTTGGGG GGAAGTTCAA CTTTGATGAG ATGCAGACCC GGAGGAGCAC CTTTGACAAC 
TTCCCGCAGT CCCTCCTCAC CGTGTTTCAG ATCCTGACCG GGGAGGACTG GAATTCGGTG 
ATGTATGATG GGATCATGGC TTATGGCGGC CCCTCTTTTC CAGGGATGTT AGTCTGTATT 
TACTTCATCA TCCTCTTCAT CTGTGGAAAT TATATCCTAC TGAACGTGTT CTTGGCCATT 
GCTGTGGACA ACCTGGCTGA CGCGGAGAGC CTCACATCTG CCCAGAAGGA GGAGGAAGAG 
GAGAAGGAGA GGAAGAAGCT AGCCAGGACC GCCAGCCCAG AGAAGAAGCA AGAGGTGGTA 
GAGAAGCCGG CCGTGGAGGA GACCAAGGAG GAGAAAATCG AGCTGAAATC CATCACGGCA 
GATGGAGAGT CCCCACCCGC CACCAAGATC AACATGGATG ACCTCCAGCC CAACGAGAAT 
GAGGACAAGA GCTCCTACCC CAACCAGGAA ACCACAGGAG AAGAGGACGA GGAGGAGCCC 
GAGATGCCCG TGGGCCCCCG CCCGCGCCCC CTCTCTGAGC TGCACCTGAA GGAGAAGGCA 
GTGCCCATGC CGGAAGCCAG CGCCTTCTTC ATCTTCAGCC CCAGCAACAG GTTCCGCCTC 
CAGTGCCATC GCATCGTCAA TGACTCCATC TTCACCAACC TGATCCTCTT CTTCATCCTG 
CTCAGCAGCA TCTCCCTGGC CGCCGAGGAC CCCGTGCAGC ACACCTCCTT CAGGAACCAC 
ATTCTGTTTT ATTTTGACAT TGTTTTCACT ACCATTTTCA CCATTGAGAT TGCCCTGAAG 
ATCCTAGGCA ACGCAGACTA TGTCTTCACT AGTATCTTTA CCTTAGAAAT TATCCTCAAG 
ATGACGGCCT ACGGGGCCTT CCTGCACAAG GGCTCCTTCT GCCGCAACTA CTTCAACATC 
CTGGACCTGC TGGTGGTCAG TGTGTCCCTC ATCTCCTTCG GCATCCAGTC CAGTGCCATC 
AACGTGGTGA AGATCCTGCG AGTCCTGAGG GTGCTCCGGC CACTGAGGGC CATCAACAGG 
GCCAAGGGGC TGAAGCACGT CGTGCAGTGC GTGTTCGTGG CCATCCGCAC CATCGGGAAC 
ATTGTGATCG TCACCACGCT GCTCCAGTTC ATGTTCGCCT GCATTGGGGT CCAGCTCTTC 
AAGGGAAAGC TCTACACCTG TTCGGACAGT TCCAAACAGA CAGAGGCTGA ATGCAAGGGG 
AACTACATAA CATACAAGGA TGGGGAGGTG GACCACCCCA TCATCCAGCC CCGCAGCTGG 
GAGAACAGCA AGTTTGACTT TGACAATGTC CTGGCAGCCA TGATGGCCCT GTTCACCGTC 
TCCACCTTCG AGGGATGGCC AGAGTTGCTG TACCGCTCCA TCGACTCTCA CACGGAGGAC 
AAGGGTCCCA TATACAACTA CCGCGTGGAG ATCTCCGTCT TCTTCATCAT CTACATCATC 
ATCATTGCCT TCTTCATGAT GAACATCTTC GTGGGTTTCG TCATCGTCAC CTTCCAGGAG 
CAGGGGGAGC AGGAATACAA GAACTGCGAG CTGGACAAGA ACCAGCGGCA GTGTGTGGAG 
TATGCCCTCA AGGCCCGGCC CCTGCGGAGG TACATCCCGA AAAACCAGCA CCAGTACAAG 
GTGTGGTACG TGGTCAACTC CACCTACTTC GAGTACCTGA TGTTCGTTCT CATTCTGCTC 
AACACCATCT GCCTGGCCAT GCAGCACTAC GGTCAGAGCT GTCTGTTCAA AATCGCCATG 
AACATCCTCA ACATGCTCTT CACCGGCCTC TTCACAGTGG AGATGGTCCT GAAGCTCATC 
GCCTTCAAGC CCAAGGGCTA CTTTAGTGAC CCCTGGAATG TCTTTGACTT CCTGATCGTA 
ATTGGCAGCG TAATTGACGT CATTCTCAGT GAGACGAATC ACTATTTCTG TGATGCATGG 
AACACATTTG ACGCCTTGAT TGTTGTGGGT AGCATTGTTG ATATAGCCAT CACCGAGGTA 
AACCCAGCTG AACATACCCA ATGCTCTCCC TTTATGAACG CGGAAGAGAA CTCTCGCATC 
TCCATCACCT TCTTCCGCCT CTTCCGGGTC ATGCGTCTGG TCAAGCTGCT GAGCCGCGGG 
GAGGGCATCC GGACACTGCT GTGGACCTTC ATCAAGTCCT TCCAGGCCCT ACCCTATGTG 
GCCCTCCTGA TCGTGATGCT GTTTTTCATC TACGCCGTGA TCGGGATGCA GGTGTTTGGC 
AAAATTGCCC TGAATGACAC GACAGAGATC AACAGGAACA ACAACTTCCA GACCTTCCCC 
CAGGCCGTGC TGCTCCTCTT CAGGTGCGCC ACTGGGGAGG CCTGGCAGGA CATCATGCTG 
GCCTGCATGC CTGGCAAGAA GTGCGCCCCC GAGTCAGAGC CGGGCAACAG CACAGAGGGG 
GAGACGCCCT GTGGCAGCAG CTTCGCCGTC TTCTACTTCA TCAGCTTCTA CATGCTCTGC 
GCCTTCCTGA TCATCAACCT CTTCGTAGCC GTCATCATGG ACAACTTTGA CTACCTGACG 
AGGGACTGGT CCATCCTGGG TCCCCACCAC CTGGACGAAT TTAAGAGAAT TTGGGCAGAG 
TATGACCCTG AAGCCAAGGG ACGCATCAAA CACCTGGATG TGGTGACCCT GCTCCGACGG 
ATTCAGCCCC CCCTGGGTTT TGGGAAGCTG TGCCCTCACC GCGTGGCTTG CAAACGCCTG 
GTCTCCATGA ACATGCCCCT GAACAGCGAC GGGACTGTCA TGTTCAATGC CACGCTGTTT 
GCCCTGGTCC GTACAGCTCT GAGGATCAAG ACGGAAGGGA ACCTGGAACA GGCCAATGAG 
GAGCTGCGGG CCATCATCAA GAAGATCTGG AAACGGACCA GCATGAAGCT GCTGGACCAA 
GTGGTGCCGC CCGCAGGAGA TGACGAGGTC ACGGTGGGCA AGTTTTACGC TACTTTCCTG 
ATCCAGGAGT ATTTCCGGAA GTTCAAGAAG CGCAAAGAGC AAGGCCTGGT GGGCAAGCCC 
TCCCAGAGGA ACGCCCTGTC CCTGCAGGCT GGCCTGCGCA CCCTGCACGA TCTGGGGCCC 
GAGATCCGCC GGGCCATCTC TGGGGACCTG ACCGCCGAGG AGGAGCTGGA CAAGGCCATG 
AAGGAGGCTG TGTCTGCAGC CTCAGAAGAT GACATCTTCA GGAGGGCCGG CGGCCTGTTC 
GGCAACCACG TGGGCTACTA CCCGAGCGAC GGCCGGGGCG CCTTCCCCCA GACCTTCACC 
ACCCAGCGCC CCCTGCACAT CAGCAAGGCA GGCAACAGCC AGGGGGACAC CGAGTCCCCC 
TCCCACGAGA AACTGGTGGA CTCCACCTTC ACCCCCAGCA GCTACTCATC CACGGGCTCC 
AACGCCAACA TCAACAACGC CAACAACACC GCCCTGGGCC GCTGCCCCCG CCCCGCGCTC 
CACTCCGGTG CTGTCAGCAC CTTGGAGGCC CACGGGCCCC CCTTGTCCCC TGCCATCCGG 
GTGCAGGAGG CCGCGTGGAG GCACAGCTCC CAGAGGTGCC CCTCCCGGGA CAGCCCGATA 
GCCATGCTGT GTCAGGAAGA GGCCTCTGGG GACGAGACCT GTGCCGTGCA CGTGGACGAG 
AACGCAGAGC ACAGCAGCGA GCCCAGCTTG GTCTCCACGG AGATGCTCTC CTACCAGGAT 
GACGAGAATC GGCAGCTGAC TCCCCCCGAG GAGGACAGGA GGGACATCCG TCTGTCCCCA 
AAGAGGGGCT TCCTGCGCTC CGCCTCCCTA GGTCGAAGGG CCTCCTTCCA CCTTGAATGT 
CTGAAGCGAC AGAAGAATCC TGGGGGAGAC GTCTCTCAGA ACACAGTCCT GCCTTTGCAC 
CTGGTTCACC ATCAGGCGCT GGCAGTGGCG GGTCTGAGCC CCCTCCTGCA GAGAAGCCAT 
CCCCCTGGGA CCCTCCCCCC ACCACGCCTC ACACCCCCCG CCACACCTGG CCCAGCCTGG 
CCTCCGCGGC CTGTGCCCAC CCTGCGGCTG GAAGGGGCCG AGTCCAGCGA CAAGCTCACC 
AGCAGCTTCC CATCCATCCA CTGCGACCCC CACATCGGGG AGCCCACGCC CTGCGGGGTT 
GTCGGCACCC CCCGGAGGGC CCGGCCCGTG TCACTCACTG TGCCCAGCCC GGCAGGGCCC 
CAGGGCCGTC CGTTCCATGG CAGTGCCAGC AGCCTGGTGG AGGCGGTCTT GATCTCGGAA 
GGATTGGGGC AGTTTGCTCA GGACCCCAGG TTCCTCGAGG CCACCACGCA GGAGCTGGCC 
GATGCCTGCG ACATGACCAT TGAGGAGATG GAGAGCGCGG CTGACGACAT CCTCAGCGGG 
GGCGCCGGGC AGAGCCCCAA CGGCACCCTG CTCCCGTGTG CAAACTGCAG GGACCCGGGG 
CCGGACCGAG CGGGGGGTGT GGAGGACGCA GCCTGGGCGC CCAGCGCCGA GCCCCGCCAG 
GGCGCAGAGG AGCCCCGGGA CAGCAGGGCC TTCGCCAGCG GCCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

CACNA1C cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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