OPLAH cDNA ORF clone, Bos taurus(cattle)

The following OPLAH gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OPLAH cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43435 NM_001001173.1
Latest version!
Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa222213 XM_025001442.1
Latest version!
Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa92439 XM_015474330.2
Latest version!
Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa25724 NM_001001173.2
Latest version!
Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa25724 XM_005215137.3
Latest version!
Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45764 XM_010811820.2
Latest version!
Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45765 XM_010811821.2
Latest version!
Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45766 XM_010811822.3
Latest version!
Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa92439 XM_015474330.1 Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
OBa45766 XM_010811822.2 Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43435
    Clone ID Related Accession (Same CDS sequence) NM_001001173.1
    Accession Version NM_001001173.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-21
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB121737.1. On Mar 29, 2005 this sequence version replaced XM_594260.1. ##Evidence-Data-START## Transcript exon combination :: AB121737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02822088, SAMN02822089 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGCCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CAACAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG TGCACCCAGG CCCGCGCGGA NCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGANCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAT GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGGCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTACCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGTCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT CCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT CCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGG AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001001173.1
    CDS7..3873
    Translation

    Target ORF information:

    RefSeq Version NM_001001173.1
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001001173.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGCCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CAACAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG TGCACCCAGG CCCGCGCGGA NCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGANCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAT GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGGCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTACCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGTCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT CCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT CCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGG AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa222213
    Clone ID Related Accession (Same CDS sequence) XM_025001442.1
    Accession Version XM_025001442.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAA 
    AACGAGGAGC TGCGGCGGCT GGTGCAGCTC ATCCAGGAGA ATCAGGAGCT GAAGCTGGTG
    CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT
    GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC
    CCAGGACTGC CGGCTGAGGA GTCGCTGCTG GACTCCAGCC CCATCACCAT GGGTGGCCCG
    GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG
    TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGTA
    GACGCCCCCA CTGAAGGCAT CCGCCGCATC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG
    CGGGACCGGC CCCTGGACAC CAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG
    ACCAATGCGC TGTTGGAACG GCAGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGCGGGTTC
    CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCGCGGACC TCTTTGACCT GGCCGTGCCC
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024857210.1
    CDS65..4219
    Translation

    Target ORF information:

    RefSeq Version XM_025001442.1
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_025001442.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCCCTCC TCAGCAGCTA CGAGGGCCTG CGGCAGGAGA TCCAGCGGCT GGCACAGGAA 
    AACGAGGAGC TGCGGCGGCT GGTGCAGCTC ATCCAGGAGA ATCAGGAGCT GAAGCTGGTG
    CTCAGGAACC GGGGCAGCAG CCTGAGCTTC TGTGGCCCCG GGCTCCTGGC TGAGGTGGCT
    GCCAGTCCCC GGCTCCCCCG GCGCAGAACC ATCAAGTTCA AGGATGTGGA GAGAGTTCTC
    CCAGGACTGC CGGCTGAGGA GTCGCTGCTG GACTCCAGCC CCATCACCAT GGGTGGCCCG
    GAGGGGCGCT TCCATTTTGC CATCGACCGT GGAGGCACCT TCACAGATGT CTTTGCCCAG
    TGCCCAGGGG GGCACGTGAG GGTCCTAAAG CTGCTTTCAG AAGACCCTGC CAACTATGTA
    GACGCCCCCA CTGAAGGCAT CCGCCGCATC CTGGAGCAGG AGGGGGGCGT GCTGCTGCCG
    CGGGACCGGC CCCTGGACAC CAGTCGCATC GCCAGCATCC GCATGGGCAC CACGGTGGCG
    ACCAATGCGC TGTTGGAACG GCAGGGAGAG CGGGTGGCAC TGCTGGTGAC GCGCGGGTTC
    CGAGACCTGC TGCACGTGGG CACCCAGGCC CGCGCGGACC TCTTTGACCT GGCCGTGCCC
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92439
    Clone ID Related Accession (Same CDS sequence) XM_015474330.2 , XM_015474330.1
    Accession Version XM_015474330.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015474330.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGTAGCG CGGTGTTTGA TTTCAGCGGC
    TCAGGGCCAG AGGTGTTCGG CAACCTCAAC GCGCCGCGGG CCATCACGCT GTCTGCGCTC
    ATCTACTGTC TTCGCTGTCT GGTTGGCCGC GACATTCCAC TCAACCAGGG CTGCCTGGCT
    CCGGTGCGCG TGGTGATTCC TAAAGGCTCC ATCCTAGACC CTTCCCCCGA CGCGGCCGTG
    GTGGGCGGCA ACGTGCTCAC GTCGCAGCGC GTGGTGGACG TCATCCTGGG GGCCTTCGGG
    GCCTGCGCCG CTTCCCAGGG CTGCATGAAC AACGTGACCT TGGGCAACGC CCACATGGGC
    TACTACGAGA CGGTGGCGGG CGGTGCGGGC GCGGGACCGG GTTGGCACGG GCGCAGCGGC
    GTGCACAGCC ACATGACCAA CACGCGCATC ACCGACCCCG AGATCCTGGA GAGCAGGTAC
    CCGGTTATCC TGCGCCGCTT CGAGCTAAGG CTGGGGTCCG GGGGTCGCGG CCGCTTCCGG
    GGCGGCGATG GCATTATCCG TGAGCTGCTT TTCCGCGAGG AGGCGCTGCT GTCCGTGCTG
    ACTGAGCGCC GCGCCTTCCA GCCGTATGGC CTGATGGGGG GTGAGCCTGG TGCCCGGGGC
    CTAAACCTAC TCATCCGGAA GGACGGCCGG ACAGTAAACC TGGGTGGGAA GACCTCGGTG
    CCCGTGTACC CCGGGGACGT GTTCTGTCTG CACACACCAG GCGGTGGGGG CTACGGGGAC
    CCGGAAGACC CCGCCCCACT GCCAGGTTCG CCCTTGCAGC CCCTAGCCTT CCCGGAGAGG
    GGCAGCGTGT ATGAGTACCG CAGGGCCCAG GAGGCTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329816.1
    CDS155..4096
    Translation

    Target ORF information:

    RefSeq Version XM_015474330.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474330.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGTAGCG CGGTGTTTGA TTTCAGCGGC
    TCAGGGCCAG AGGTGTTCGG CAACCTCAAC GCGCCGCGGG CCATCACGCT GTCTGCGCTC
    ATCTACTGTC TTCGCTGTCT GGTTGGCCGC GACATTCCAC TCAACCAGGG CTGCCTGGCT
    CCGGTGCGCG TGGTGATTCC TAAAGGCTCC ATCCTAGACC CTTCCCCCGA CGCGGCCGTG
    GTGGGCGGCA ACGTGCTCAC GTCGCAGCGC GTGGTGGACG TCATCCTGGG GGCCTTCGGG
    GCCTGCGCCG CTTCCCAGGG CTGCATGAAC AACGTGACCT TGGGCAACGC CCACATGGGC
    TACTACGAGA CGGTGGCGGG CGGTGCGGGC GCGGGACCGG GTTGGCACGG GCGCAGCGGC
    GTGCACAGCC ACATGACCAA CACGCGCATC ACCGACCCCG AGATCCTGGA GAGCAGGTAC
    CCGGTTATCC TGCGCCGCTT CGAGCTAAGG CTGGGGTCCG GGGGTCGCGG CCGCTTCCGG
    GGCGGCGATG GCATTATCCG TGAGCTGCTT TTCCGCGAGG AGGCGCTGCT GTCCGTGCTG
    ACTGAGCGCC GCGCCTTCCA GCCGTATGGC CTGATGGGGG GTGAGCCTGG TGCCCGGGGC
    CTAAACCTAC TCATCCGGAA GGACGGCCGG ACAGTAAACC TGGGTGGGAA GACCTCGGTG
    CCCGTGTACC CCGGGGACGT GTTCTGTCTG CACACACCAG GCGGTGGGGG CTACGGGGAC
    CCGGAAGACC CCGCCCCACT GCCAGGTTCG CCCTTGCAGC CCCTAGCCTT CCCGGAGAGG
    GGCAGCGTGT ATGAGTACCG CAGGGCCCAG GAGGCTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa92439
    Clone ID Related Accession (Same CDS sequence) XM_015474330.2 , XM_015474330.1
    Accession Version XM_015474330.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3942bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGTAGCG CGGTGTTTGA TTTCAGCGGC
    TCAGGGCCAG AGGTGTTCGG CAACCTCAAC GCGCCGCGGG CCATCACGCT GTCTGCGCTC
    ATCTACTGTC TTCGCTGTCT GGTTGGCCGC GACATTCCAC TCAACCAGGG CTGCCTGGCT
    CCGGTGCGCG TGGTGATTCC TAAAGGCTCC ATCCTAGACC CTTCCCCCGA CGCGGCCGTG
    GTGGGCGGCA ACGTGCTCAC GTCGCAGCGC GTGGTGGACG TCATCCTGGG GGCCTTCGGG
    GCCTGCGCCG CTTCCCAGGG CTGCATGAAC AACGTGACCT TGGGCAACGC CCACATGGGC
    TACTACGAGA CGGTGGCGGG CGGTGCGGGC GCGGGACCGG GTTGGCACGG GCGCAGCGGC
    GTGCACAGCC ACATGACCAA CACGCGCATC ACCGACCCCG AGATCCTGGA GAGCAGGTAC
    CCGGTTATCC TGCGCCGCTT CGAGCTAAGG CTGGGGTCCG GGGGTCGCGG CCGCTTCCGG
    GGCGGCGATG GCATTATCCG TGAGCTGCTT TTCCGCGAGG AGGCGCTGCT GTCCGTGCTG
    ACTGAGCGCC GCGCCTTCCA GCCGTATGGC CTGATGGGGG GTGAGCCTGG TGCCCGGGGC
    CTAAACCTAC TCATCCGGAA GGACGGCCGG ACAGTAAACC TGGGTGGGAA GACCTCGGTG
    CCCGTGTACC CCGGGGACGT GTTCTGTCTG CACACACCAG GCGGTGGGGG CTACGGGGAC
    CCGGAAGACC CCGCCCCACT GCCAGGTTCG CCCTTGCAGC CCCTAGCCTT CCCGGAGAGG
    GGCAGCGTGT ATGAGTACCG CAGGGCCCAG GAGGCTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015329816.1
    CDS146..4087
    Translation

    Target ORF information:

    RefSeq Version XM_015474330.1
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015474330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGTAGCG CGGTGTTTGA TTTCAGCGGC
    TCAGGGCCAG AGGTGTTCGG CAACCTCAAC GCGCCGCGGG CCATCACGCT GTCTGCGCTC
    ATCTACTGTC TTCGCTGTCT GGTTGGCCGC GACATTCCAC TCAACCAGGG CTGCCTGGCT
    CCGGTGCGCG TGGTGATTCC TAAAGGCTCC ATCCTAGACC CTTCCCCCGA CGCGGCCGTG
    GTGGGCGGCA ACGTGCTCAC GTCGCAGCGC GTGGTGGACG TCATCCTGGG GGCCTTCGGG
    GCCTGCGCCG CTTCCCAGGG CTGCATGAAC AACGTGACCT TGGGCAACGC CCACATGGGC
    TACTACGAGA CGGTGGCGGG CGGTGCGGGC GCGGGACCGG GTTGGCACGG GCGCAGCGGC
    GTGCACAGCC ACATGACCAA CACGCGCATC ACCGACCCCG AGATCCTGGA GAGCAGGTAC
    CCGGTTATCC TGCGCCGCTT CGAGCTAAGG CTGGGGTCCG GGGGTCGCGG CCGCTTCCGG
    GGCGGCGATG GCATTATCCG TGAGCTGCTT TTCCGCGAGG AGGCGCTGCT GTCCGTGCTG
    ACTGAGCGCC GCGCCTTCCA GCCGTATGGC CTGATGGGGG GTGAGCCTGG TGCCCGGGGC
    CTAAACCTAC TCATCCGGAA GGACGGCCGG ACAGTAAACC TGGGTGGGAA GACCTCGGTG
    CCCGTGTACC CCGGGGACGT GTTCTGTCTG CACACACCAG GCGGTGGGGG CTACGGGGAC
    CCGGAAGACC CCGCCCCACT GCCAGGTTCG CCCTTGCAGC CCCTAGCCTT CCCGGAGAGG
    GGCAGCGTGT ATGAGTACCG CAGGGCCCAG GAGGCTGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25724
    Clone ID Related Accession (Same CDS sequence) XM_005215137.3 , NM_001001173.2
    Accession Version NM_001001173.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000014.1, EV743436.1 and AW461711.1. On or before Jan 19, 2018 this sequence version replaced XM_005215137.3, NM_001001173.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: SRR6764281.29680.1, SRR6764281.30795.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145414 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGCAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCGCGGA CCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTTCCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001001173.2
    CDS65..3931
    Translation

    Target ORF information:

    RefSeq Version NM_001001173.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001001173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGCAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCGCGGA CCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTTCCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa25724
    Clone ID Related Accession (Same CDS sequence) XM_005215137.3 , NM_001001173.2
    Accession Version XM_005215137.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3867bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005215137.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGCAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCGCGGA CCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTTCCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005215194.1
    CDS160..4026
    Translation

    Target ORF information:

    RefSeq Version XM_005215137.3
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005215137.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGGTGGCC CGGAGGGGCG CTTCCATTTT GCCATCGACC GTGGAGGCAC CTTCACAGAT 
    GTCTTTGCCC AGTGCCCAGG GGGGCACGTG AGGGTCCTAA AGCTGCTTTC AGAAGACCCT
    GCCAACTATG TAGACGCCCC CACTGAAGGC ATCCGCCGCA TCCTGGAGCA GGAGGGGGGC
    GTGCTGCTGC CGCGGGACCG GCCCCTGGAC ACCAGTCGCA TCGCCAGCAT CCGCATGGGC
    ACCACGGTGG CGACCAATGC GCTGTTGGAA CGGCAGGGAG AGCGGGTGGC ACTGCTGGTG
    ACGCGCGGGT TCCGAGACCT GCTGCACGTG GGCACCCAGG CCCGCGCGGA CCTCTTTGAC
    CTGGCCGTGC CCATGCCCGA GACGCTGTAC GAGGAGGTGC TGGAAGTGGA CGAACGGGTG
    GTGCTCTATC GAGGGGAGCC GGGTGCTGGG ACACCCGTGA AAGGCTGCAC AGGGGACCTA
    CTGGAAGTGC AGCAGCCTGT GGACCTGGGG GGCCTGCGAG GGAAGCTGGA AGGTCTCCTG
    TCCCGGGGCA TCCGTAGCTT GGCCGTGGTG CTCATGCACT CGTACACGTG GGCCCAGCAC
    GAGCAGCAGG TGGGCGCCCT GGCCCGGGAA CTGGGCTTCA CGCATGTGTC GCTGTCCTCG
    GAGGCCATGC CCATGGTGCG CATTGTGCCC CGGGGGCACA CGGCCTGTGC TGACGCCTAT
    CTCACGCCCA CCATCCAGCG CTACGTGCAG GGCTTCCGAC GTGGCTTCCA GGGTCAGCTC
    AAGGACGTGC AGGTGCTGTT CATGCGCTCC GACGGTGGGC TGGCGCCCAT GGACTCCTTC
    AGCGGCTCCC GCGCTGTGCT CTCCGGCCCT GCGGGGGGCG TGGTTGGTTA CTCAGCCACC
    ACCTACCGGG TGGAGGGCGG CCAGCCAGTC ATCGGCTTTG ACATGGGAGG CACGTCTACC
    GACGTGAGCC GCTATGCTGG CGAGTTTGAG CATGTTTTCG AGGCCAGCAC GGCTGGTGTC
    ACCCTCCAGG CCCCCCAGCT GGATATCAAC ACTGTGGCAG CCGGTGGGGG CTCCCGCCTC
    TTTTTCAGGT CGGGCCTCTT CGTGGTGGGG CCAGAGTCTG CGGGAGCCCA CCCTGGCCCC
    GCCTGCTACC GAAAAGGGGG CCCTGTGACA GTGACGGATG CTAATCTGGT CCTGGGTCGC
    CTGCTGCCTG CCTCCTTCCC CTGCATTTTT GGGCCGGGAG AGGACCAGCC ACTGTCCCCG
    GAGGCGTCCC GAAAGGCCCT GGAGGCTGTG GCCACGGAGG TCAACAGCTT CCTGACCAAT
    GGGCCTTGCC CGGCCTCCCC GCTGAGCCTG GAGGAGGTGG CCATGGGGTT TGTGCGTGTG
    GCCAACGAGG CCATGTGTCG GCCCATCCGT GCACTCACGC AGGCACGAGG TCATGACCCC
    TCGGCCCACG TGCTGGCCTG CTTTGGGGGA GCTGGTGGGC AGCATGCTTG TGCCATTGCC
    CGGGCCCTGG GCATGGACAC TGTGCACATT CACAGGCACA GTGGGCTGCT GTCGGCGCTG
    GGGCTGGCCC TGGCTGATGT GGTGCATGAG GCGCAGGAGC CCTGCTCCCT GCCATATGCT
    CCCGAGACCT TTGCGCAGCT GGACCAGAGG CTGGGCCGCC TGGAGGAGCA GTGTGTGGAG
    GCCCTGCGGG CCCAGGGCTT CCCCAGGTCC CAGATCAGCA CCGAGAGCTT CCTGCACCTG
    CGCTACCAGG GCACGGACTG CGCCCTGATG GTGTCTGCCC ACCAGCACCC GGCCAGTGCC
    CGCTCACCCC GAGCTGGTGA CTTTGGGGCA GCCTTTGTGG AGAGGTACAT GAGGGAGTTC
    GGCTTCATTA TTCCCGAGCG GCCGGTGGTG GTGGATGACG TGCGGGTCAG GGGCACCGGC
    AGCAGCAGCC TTCGCCTCGA GGACGTCCCA AAAGCCCATA GTGGGCCTCC CCGGGTAGAT
    AAGATGACCC AGTGCTACTT TGAGGGGGGC TACCAGGAGA CCCCTGTGTA CCTGTTGGGA
    GAGCTGGGCT GTGGGCACAA GCTTCAGGGG CCCTGCCTCA TCATTGACAG CAACAGTACC
    ATCCTGGTGG AGCCAGGCTG CCAGGCAGAG GTGACTGAGA CTGGGGACAT CCGCATCTCT
    GTGGGGGCTG AGACAGCCAG CGTGGTGGGC ACGCAGCTTG ACCCCATCCA CCTTTCCATC
    TTCTCCCACC GCTTCATGAG CATTGCTGAG CAGATGGGCC GCATCCTGCA GCGCACGGCC
    ATCTCCACCA ACATCAAGGA GCGTCTGGAC TTCTCCTGCG CCCTCTTTGG GCCCGACGGG
    GGGCTGGTCT CCAACGCCCC TCACATCCCT GTGCACCTGG GTGCCATGCA GGAGACGGTG
    CAGTTCCAGA TTCAGCAGTT GGGGGCTGAT CTCCACCCCG GCGATGTGCT GCTGAGCAAC
    CACCCCAGCG CGGGGGGCAG CCACTTGCCA GACCTGACTG TCATCACACC GGTGTTTTGG
    CCGGGTCAGA CACGGCCTGT GTTCTATGTG GCCAGCCGTG GGCACCATGC AGACATTGGG
    GGCATCACAC CAGGCTCCAT GCCCCCCCAC TCCACCAGCC TGCAGCAGGA GGGCGCGGTC
    TTCCTTTCCT TCAAACTTGT GCATGGGGGA GTCTTCCAGG AAGAGGCGGT GACTGAAGCC
    CTGCGGGCGC CAGGCAAGAT TCCAGGCTGC AGCGGGACAC GGAACCTGCA CGACAACCTG
    TCGGATCTGC GCGCCCAGGT GGCGGCCAAC CAGAAGGGCA TCCAGCTGGT GGGCGAGCTC
    ATCGGGCAGT ACGGCCTGGA CGTGGTGCAG GCCTACATGG GCCACATTCA GGCAAACGCG
    GAGCTCGCTG TGAGGGACAT GCTCCGGGCC TTTGGAACTG CCCGGCAGGC CCGGGGCCTG
    CCCCTGGAGG TGTCTGCAGA GGACCACATG GACGACGGTT CCCCTATCCG ACTCCGAGTG
    CAGATCAACA TGAGTCAGGG TAGCGCGGTG TTTGATTTCA GCGGCTCAGG GCCAGAGGTG
    TTCGGCAACC TCAACGCGCC GCGGGCCATC ACGCTGTCTG CGCTCATCTA CTGTCTTCGC
    TGTCTGGTTG GCCGCGACAT TCCACTCAAC CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45764
    Clone ID Related Accession (Same CDS sequence) XM_010811820.2
    Accession Version XM_010811820.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3813bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811820.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGCGCTAC GTGCAGGGCT TCCGACGTGG CTTCCAGGGT
    CAGCTCAAGG ACGTGCAGGT GCTGTTCATG CGCTCCGACG GTGGGCTGGC GCCCATGGAC
    TCCTTCAGCG GCTCCCGCGC TGTGCTCTCC GGCCCTGCGG GGGGCGTGGT TGGTTACTCA
    GCCACCACCT ACCGGGTGGA GGGCGGCCAG CCAGTCATCG GCTTTGACAT GGGAGGCACG
    TCTACCGACG TGAGCCGCTA TGCTGGCGAG TTTGAGCATG TTTTCGAGGC CAGCACGGCT
    GGTGTCACCC TCCAGGCCCC CCAGCTGGAT ATCAACACTG TGGCAGCCGG TGGGGGCTCC
    CGCCTCTTTT TCAGGTCGGG CCTCTTCGTG GTGGGGCCAG AGTCTGCGGG AGCCCACCCT
    GGCCCCGCCT GCTACCGAAA AGGGGGCCCT GTGACAGTGA CGGATGCTAA TCTGGTCCTG
    GGTCGCCTGC TGCCTGCCTC CTTCCCCTGC ATTTTTGGGC CGGGAGAGGA CCAGCCACTG
    TCCCCGGAGG CGTCCCGAAA GGCCCTGGAG GCTGTGGCCA CGGAGGTCAA CAGCTTCCTG
    ACCAATGGGC CTTGCCCGGC CTCCCCGCTG AGCCTGGAGG AGGTGGCCAT GGGGTTTGTG
    CGTGTGGCCA ACGAGGCCAT GTGTCGGCCC ATCCGTGCAC TCACGCAGGC ACGAGGTCAT
    GACCCCTCGG CCCACGTGCT GGCCTGCTTT GGGGGAGCTG GTGGGCAGCA TGCTTGTGCC
    ATTGCCCGGG CCCTGGGCAT GGACACTGTG CACATTCACA GGCACAGTGG GCTGCTGTCG
    GCGCTGGGGC TGGCCCTGGC TGATGTGGTG CATGAGGCGC AGGAGCCCTG CTCCCTGCCA
    TATGCTCCCG AGACCTTTGC GCAGCTGGAC CAGAGGCTGG GCCGCCTGGA GGAGCAGTGT
    GTGGAGGCCC TGCGGGCCCA GGGCTTCCCC AGGTCCCAGA TCAGCACCGA GAGCTTCCTG
    CACCTGCGCT ACCAGGGCAC GGACTGCGCC CTGATGGTGT CTGCCCACCA GCACCCGGCC
    AGTGCCCGCT CACCCCGAGC TGGTGACTTT GGGGCAGCCT TTGTGGAGAG GTACATGAGG
    GAGTTCGGCT TCATTATTCC CGAGCGGCCG GTGGTGGTGG ATGACGTGCG GGTCAGGGGC
    ACCGGCAGCA GCAGCCTTCG CCTCGAGGAC GTCCCAAAAG CCCATAGTGG GCCTCCCCGG
    GTAGATAAGA TGACCCAGTG CTACTTTGAG GGGGGCTACC AGGAGACCCC TGTGTACCTG
    TTGGGAGAGC TGGGCTGTGG GCACAAGCTT CAGGGGCCCT GCCTCATCAT TGACAGCAAC
    AGTACCATCC TGGTGGAGCC AGGCTGCCAG GCAGAGGTGA CTGAGACTGG GGACATCCGC
    ATCTCTGTGG GGGCTGAGAC AGCCAGCGTG GTGGGCACGC AGCTTGACCC CATCCACCTT
    TCCATCTTCT CCCACCGCTT CATGAGCATT GCTGAGCAGA TGGGCCGCAT CCTGCAGCGC
    ACGGCCATCT CCACCAACAT CAAGGAGCGT CTGGACTTCT CCTGCGCCCT CTTTGGGCCC
    GACGGGGGGC TGGTCTCCAA CGCCCCTCAC ATCCCTGTGC ACCTGGGTGC CATGCAGGAG
    ACGGTGCAGT TCCAGATTCA GCAGTTGGGG GCTGATCTCC ACCCCGGCGA TGTGCTGCTG
    AGCAACCACC CCAGCGCGGG GGGCAGCCAC TTGCCAGACC TGACTGTCAT CACACCGGTG
    TTTTGGCCGG GTCAGACACG GCCTGTGTTC TATGTGGCCA GCCGTGGGCA CCATGCAGAC
    ATTGGGGGCA TCACACCAGG CTCCATGCCC CCCCACTCCA CCAGCCTGCA GCAGGAGGGC
    GCGGTCTTCC TTTCCTTCAA ACTTGTGCAT GGGGGAGTCT TCCAGGAAGA GGCGGTGACT
    GAAGCCCTGC GGGCGCCAGG CAAGATTCCA GGCTGCAGCG GGACACGGAA CCTGCACGAC
    AACCTGTCGG ATCTGCGCGC CCAGGTGGCG GCCAACCAGA AGGGCATCCA GCTGGTGGGC
    GAGCTCATCG GGCAGTACGG CCTGGACGTG GTGCAGGCCT ACATGGGCCA CATTCAGGCA
    AACGCGGAGC TCGCTGTGAG GGACATGCTC CGGGCCTTTG GAACTGCCCG GCAGGCCCGG
    GGCCTGCCCC TGGAGGTGTC TGCAGAGGAC CACATGGACG ACGGTTCCCC TATCCGACTC
    CGAGTGCAGA TCAACATGAG TCAGGGTAGC GCGGTGTTTG ATTTCAGCGG CTCAGGGCCA
    GAGGTGTTCG GCAACCTCAA CGCGCCGCGG GCCATCACGC TGTCTGCGCT CATCTACTGT
    CTTCGCTGTC TGGTTGGCCG CGACATTCCA CTCAACCAGG GCTGCCTGGC TCCGGTGCGC
    GTGGTGATTC CTAAAGGCTC CATCCTAGAC CCTTCCCCCG ACGCGGCCGT GGTGGGCGGC
    AACGTGCTCA CGTCGCAGCG CGTGGTGGAC GTCATCCTGG GGGCCTTCGG GGCCTGCGCC
    GCTTCCCAGG GCTGCATGAA CAACGTGACC TTGGGCAACG CCCACATGGG CTACTACGAG
    ACGGTGGCGG GCGGTGCGGG CGCGGGACCG GGTTGGCACG GGCGCAGCGG CGTGCACAGC
    CACATGACCA ACACGCGCAT CACCGACCCC GAGATCCTGG AGAGCAGGTA CCCGGTTATC
    CTGCGCCGCT TCGAGCTAAG GCTGGGGTCC GGGGGTCGCG GCCGCTTCCG GGGCGGCGAT
    GGCATTATCC GTGAGCTGCT TTTCCGCGAG GAGGCGCTGC TGTCCGTGCT GACTGAGCGC
    CGCGCCTTCC AGCCGTATGG CCTGATGGGG GGTGAGCCTG GTGCCCGGGG CCTAAACCTA
    CTCATCCGGA AGGACGGCCG GACAGTAAAC CTGGGTGGGA AGACCTCGGT GCCCGTGTAC
    CCCGGGGACG TGTTCTGTCT GCACACACCA GGCGGTGGGG GCTACGGGGA CCCGGAAGAC
    CCCGCCCCAC TGCCAGGTTC GCCCTTGCAG CCCCTAGCCT TCCCGGAGAG GGGCAGCGTG
    TATGAGTACC GCAGGGCCCA GGAGGCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810122.1
    CDS146..3958
    Translation

    Target ORF information:

    RefSeq Version XM_010811820.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811820.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGCGCTAC GTGCAGGGCT TCCGACGTGG CTTCCAGGGT
    CAGCTCAAGG ACGTGCAGGT GCTGTTCATG CGCTCCGACG GTGGGCTGGC GCCCATGGAC
    TCCTTCAGCG GCTCCCGCGC TGTGCTCTCC GGCCCTGCGG GGGGCGTGGT TGGTTACTCA
    GCCACCACCT ACCGGGTGGA GGGCGGCCAG CCAGTCATCG GCTTTGACAT GGGAGGCACG
    TCTACCGACG TGAGCCGCTA TGCTGGCGAG TTTGAGCATG TTTTCGAGGC CAGCACGGCT
    GGTGTCACCC TCCAGGCCCC CCAGCTGGAT ATCAACACTG TGGCAGCCGG TGGGGGCTCC
    CGCCTCTTTT TCAGGTCGGG CCTCTTCGTG GTGGGGCCAG AGTCTGCGGG AGCCCACCCT
    GGCCCCGCCT GCTACCGAAA AGGGGGCCCT GTGACAGTGA CGGATGCTAA TCTGGTCCTG
    GGTCGCCTGC TGCCTGCCTC CTTCCCCTGC ATTTTTGGGC CGGGAGAGGA CCAGCCACTG
    TCCCCGGAGG CGTCCCGAAA GGCCCTGGAG GCTGTGGCCA CGGAGGTCAA CAGCTTCCTG
    ACCAATGGGC CTTGCCCGGC CTCCCCGCTG AGCCTGGAGG AGGTGGCCAT GGGGTTTGTG
    CGTGTGGCCA ACGAGGCCAT GTGTCGGCCC ATCCGTGCAC TCACGCAGGC ACGAGGTCAT
    GACCCCTCGG CCCACGTGCT GGCCTGCTTT GGGGGAGCTG GTGGGCAGCA TGCTTGTGCC
    ATTGCCCGGG CCCTGGGCAT GGACACTGTG CACATTCACA GGCACAGTGG GCTGCTGTCG
    GCGCTGGGGC TGGCCCTGGC TGATGTGGTG CATGAGGCGC AGGAGCCCTG CTCCCTGCCA
    TATGCTCCCG AGACCTTTGC GCAGCTGGAC CAGAGGCTGG GCCGCCTGGA GGAGCAGTGT
    GTGGAGGCCC TGCGGGCCCA GGGCTTCCCC AGGTCCCAGA TCAGCACCGA GAGCTTCCTG
    CACCTGCGCT ACCAGGGCAC GGACTGCGCC CTGATGGTGT CTGCCCACCA GCACCCGGCC
    AGTGCCCGCT CACCCCGAGC TGGTGACTTT GGGGCAGCCT TTGTGGAGAG GTACATGAGG
    GAGTTCGGCT TCATTATTCC CGAGCGGCCG GTGGTGGTGG ATGACGTGCG GGTCAGGGGC
    ACCGGCAGCA GCAGCCTTCG CCTCGAGGAC GTCCCAAAAG CCCATAGTGG GCCTCCCCGG
    GTAGATAAGA TGACCCAGTG CTACTTTGAG GGGGGCTACC AGGAGACCCC TGTGTACCTG
    TTGGGAGAGC TGGGCTGTGG GCACAAGCTT CAGGGGCCCT GCCTCATCAT TGACAGCAAC
    AGTACCATCC TGGTGGAGCC AGGCTGCCAG GCAGAGGTGA CTGAGACTGG GGACATCCGC
    ATCTCTGTGG GGGCTGAGAC AGCCAGCGTG GTGGGCACGC AGCTTGACCC CATCCACCTT
    TCCATCTTCT CCCACCGCTT CATGAGCATT GCTGAGCAGA TGGGCCGCAT CCTGCAGCGC
    ACGGCCATCT CCACCAACAT CAAGGAGCGT CTGGACTTCT CCTGCGCCCT CTTTGGGCCC
    GACGGGGGGC TGGTCTCCAA CGCCCCTCAC ATCCCTGTGC ACCTGGGTGC CATGCAGGAG
    ACGGTGCAGT TCCAGATTCA GCAGTTGGGG GCTGATCTCC ACCCCGGCGA TGTGCTGCTG
    AGCAACCACC CCAGCGCGGG GGGCAGCCAC TTGCCAGACC TGACTGTCAT CACACCGGTG
    TTTTGGCCGG GTCAGACACG GCCTGTGTTC TATGTGGCCA GCCGTGGGCA CCATGCAGAC
    ATTGGGGGCA TCACACCAGG CTCCATGCCC CCCCACTCCA CCAGCCTGCA GCAGGAGGGC
    GCGGTCTTCC TTTCCTTCAA ACTTGTGCAT GGGGGAGTCT TCCAGGAAGA GGCGGTGACT
    GAAGCCCTGC GGGCGCCAGG CAAGATTCCA GGCTGCAGCG GGACACGGAA CCTGCACGAC
    AACCTGTCGG ATCTGCGCGC CCAGGTGGCG GCCAACCAGA AGGGCATCCA GCTGGTGGGC
    GAGCTCATCG GGCAGTACGG CCTGGACGTG GTGCAGGCCT ACATGGGCCA CATTCAGGCA
    AACGCGGAGC TCGCTGTGAG GGACATGCTC CGGGCCTTTG GAACTGCCCG GCAGGCCCGG
    GGCCTGCCCC TGGAGGTGTC TGCAGAGGAC CACATGGACG ACGGTTCCCC TATCCGACTC
    CGAGTGCAGA TCAACATGAG TCAGGGTAGC GCGGTGTTTG ATTTCAGCGG CTCAGGGCCA
    GAGGTGTTCG GCAACCTCAA CGCGCCGCGG GCCATCACGC TGTCTGCGCT CATCTACTGT
    CTTCGCTGTC TGGTTGGCCG CGACATTCCA CTCAACCAGG GCTGCCTGGC TCCGGTGCGC
    GTGGTGATTC CTAAAGGCTC CATCCTAGAC CCTTCCCCCG ACGCGGCCGT GGTGGGCGGC
    AACGTGCTCA CGTCGCAGCG CGTGGTGGAC GTCATCCTGG GGGCCTTCGG GGCCTGCGCC
    GCTTCCCAGG GCTGCATGAA CAACGTGACC TTGGGCAACG CCCACATGGG CTACTACGAG
    ACGGTGGCGG GCGGTGCGGG CGCGGGACCG GGTTGGCACG GGCGCAGCGG CGTGCACAGC
    CACATGACCA ACACGCGCAT CACCGACCCC GAGATCCTGG AGAGCAGGTA CCCGGTTATC
    CTGCGCCGCT TCGAGCTAAG GCTGGGGTCC GGGGGTCGCG GCCGCTTCCG GGGCGGCGAT
    GGCATTATCC GTGAGCTGCT TTTCCGCGAG GAGGCGCTGC TGTCCGTGCT GACTGAGCGC
    CGCGCCTTCC AGCCGTATGG CCTGATGGGG GGTGAGCCTG GTGCCCGGGG CCTAAACCTA
    CTCATCCGGA AGGACGGCCG GACAGTAAAC CTGGGTGGGA AGACCTCGGT GCCCGTGTAC
    CCCGGGGACG TGTTCTGTCT GCACACACCA GGCGGTGGGG GCTACGGGGA CCCGGAAGAC
    CCCGCCCCAC TGCCAGGTTC GCCCTTGCAG CCCCTAGCCT TCCCGGAGAG GGGCAGCGTG
    TATGAGTACC GCAGGGCCCA GGAGGCTGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45765
    Clone ID Related Accession (Same CDS sequence) XM_010811821.2
    Accession Version XM_010811821.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3807bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811821.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810123.1
    CDS150..3956
    Translation

    Target ORF information:

    RefSeq Version XM_010811821.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811821.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGACCCACT GCTTCTGGCC AGTTCTCCCA GGACTGCCGG CTGAGGAGTC GCTGCTGGAC 
    TCCAGCCCCA TCACCATGGG TGGCCCGGAG GGGCGCTTCC ATTTTGCCAT CGACCGTGGA
    GGCACCTTCA CAGATGTCTT TGCCCAGTGC CCAGGGGGGC ACGTGAGGGT CCTAAAGCTG
    CTTTCAGAAG ACCCTGCCAA CTATGTAGAC GCCCCCACTG AAGGCATCCG CCGCATCCTG
    GAGCAGGAGG GGGGCGTGCT GCTGCCGCGG GACCGGCCCC TGGACACCAG TCGCATCGCC
    AGCATCCGCA TGGGCACCAC GGTGGCGACC AATGCGCTGT TGGAACGGCA GGGAGAGCGG
    GTGGCACTGC TGGTGACGCG CGGGTTCCGA GACCTGCTGC ACGTGGGCAC CCAGGCCCGC
    GCGGACCTCT TTGACCTGGC CGTGCCCATG CCCGAGACGC TGTACGAGGA GGTGCTGGAA
    GTGGACGAAC GGGTGGTGCT CTATCGAGGG GAGCCGGGTG CTGGGACACC CGTGAAAGGC
    TGCACAGGGG ACCTACTGGA AGTGCAGCAG CCTGTGGACC TGGGGGGCCT GCGAGGGAAG
    CTGGAAGGTC TCCTGTCCCG GGGCATCCGT AGCTTGGCCG TGGTGCTCAT GCACTCGTAC
    ACGTGGGCCC AGCACGAGCA GCAGGTGGGC GCCCTGGCCC GGGAACTGGG CTTCACGCAT
    GTGTCGCTGT CCTCGGAGGC CATGCCCATG GTGCGCATTG TGCCCCGGGG GCACACGGCC
    TGTGCTGACG CCTATCTCAC GCCCACCATC CAGCGCTACG TGCAGGGCTT CCGACGTGGC
    TTCCAGGGTC AGCTCAAGGA CGTGCAGGTG CTGTTCATGC GCTCCGACGG TGGGCTGGCG
    CCCATGGACT CCTTCAGCGG CTCCCGCGCT GTGCTCTCCG GCCCTGCGGG GGGCGTGGTT
    GGTTACTCAG CCACCACCTA CCGGGTGGAG GGCGGCCAGC CAGTCATCGG CTTTGACATG
    GGAGGCACGT CTACCGACGT GAGCCGCTAT GCTGGCGAGT TTGAGCATGT TTTCGAGGCC
    AGCACGGCTG GTGTCACCCT CCAGGCCCCC CAGCTGGATA TCAACACTGT GGCAGCCGGT
    GGGGGCTCCC GCCTCTTTTT CAGGTCGGGC CTCTTCGTGG TGGGGCCAGA GTCTGCGGGA
    GCCCACCCTG GCCCCGCCTG CTACCGAAAA GGGGGCCCTG TGACAGTGAC GGATGCTAAT
    CTGGTCCTGG GTCGCCTGCT GCCTGCCTCC TTCCCCTGCA TTTTTGGGCC GGGAGAGGAC
    CAGCCACTGT CCCCGGAGGC GTCCCGAAAG GCCCTGGAGG CTGTGGCCAC GGAGGTCAAC
    AGCTTCCTGA CCAATGGGCC TTGCCCGGCC TCCCCGCTGA GCCTGGAGGA GGTGGCCATG
    GGGTTTGTGC GTGTGGCCAA CGAGGCCATG TGTCGGCCCA TCCGTGCACT CACGCAGGCA
    CGAGGTCATG ACCCCTCGGC CCACGTGCTG GCCTGCTTTG GGGGAGCTGG TGGGCAGCAT
    GCTTGTGCCA TTGCCCGGGC CCTGGGCATG GACACTGTGC ACATTCACAG GCACAGTGGG
    CTGCTGTCGG CGCTGGGGCT GGCCCTGGCT GATGTGGTGC ATGAGGCGCA GGAGCCCTGC
    TCCCTGCCAT ATGCTCCCGA GACCTTTGCG CAGCTGGACC AGAGGCTGGG CCGCCTGGAG
    GAGCAGTGTG TGGAGGCCCT GCGGGCCCAG GGCTTCCCCA GGTCCCAGAT CAGCACCGAG
    AGCTTCCTGC ACCTGCGCTA CCAGGGCACG GACTGCGCCC TGATGGTGTC TGCCCACCAG
    CACCCGGCCA GTGCCCGCTC ACCCCGAGCT GGTGACTTTG GGGCAGCCTT TGTGGAGAGG
    TACATGAGGG AGTTCGGCTT CATTATTCCC GAGCGGCCGG TGGTGGTGGA TGACGTGCGG
    GTCAGGGGCA CCGGCAGCAG CAGCCTTCGC CTCGAGGACG TCCCAAAAGC CCATAGTGGG
    CCTCCCCGGG TAGATAAGAT GACCCAGTGC TACTTTGAGG GGGGCTACCA GGAGACCCCT
    GTGTACCTGT TGGGAGAGCT GGGCTGTGGG CACAAGCTTC AGGGGCCCTG CCTCATCATT
    GACAGCAACA GTACCATCCT GGTGGAGCCA GGCTGCCAGG CAGAGGTGAC TGAGACTGGG
    GACATCCGCA TCTCTGTGGG GGCTGAGACA GCCAGCGTGG TGGGCACGCA GCTTGACCCC
    ATCCACCTTT CCATCTTCTC CCACCGCTTC ATGAGCATTG CTGAGCAGAT GGGCCGCATC
    CTGCAGCGCA CGGCCATCTC CACCAACATC AAGGAGCGTC TGGACTTCTC CTGCGCCCTC
    TTTGGGCCCG ACGGGGGGCT GGTCTCCAAC GCCCCTCACA TCCCTGTGCA CCTGGGTGCC
    ATGCAGGAGA CGGTGCAGTT CCAGATTCAG CAGTTGGGGG CTGATCTCCA CCCCGGCGAT
    GTGCTGCTGA GCAACCACCC CAGCGCGGGG GGCAGCCACT TGCCAGACCT GACTGTCATC
    ACACCGGTGT TTTGGCCGGG TCAGACACGG CCTGTGTTCT ATGTGGCCAG CCGTGGGCAC
    CATGCAGACA TTGGGGGCAT CACACCAGGC TCCATGCCCC CCCACTCCAC CAGCCTGCAG
    CAGGAGGGCG CGGTCTTCCT TTCCTTCAAA CTTGTGCATG GGGGAGTCTT CCAGGAAGAG
    GCGGTGACTG AAGCCCTGCG GGCGCCAGGC AAGATTCCAG GCTGCAGCGG GACACGGAAC
    CTGCACGACA ACCTGTCGGA TCTGCGCGCC CAGGTGGCGG CCAACCAGAA GGGCATCCAG
    CTGGTGGGCG AGCTCATCGG GCAGTACGGC CTGGACGTGG TGCAGGCCTA CATGGGCCAC
    ATTCAGGCAA ACGCGGAGCT CGCTGTGAGG GACATGCTCC GGGCCTTTGG AACTGCCCGG
    CAGGCCCGGG GCCTGCCCCT GGAGGTGTCT GCAGAGGACC ACATGGACGA CGGTTCCCCT
    ATCCGACTCC GAGTGCAGAT CAACATGAGT CAGGGCTGCC TGGCTCCGGT GCGCGTGGTG
    ATTCCTAAAG GCTCCATCCT AGACCCTTCC CCCGACGCGG CCGTGGTGGG CGGCAACGTG
    CTCACGTCGC AGCGCGTGGT GGACGTCATC CTGGGGGCCT TCGGGGCCTG CGCCGCTTCC
    CAGGGCTGCA TGAACAACGT GACCTTGGGC AACGCCCACA TGGGCTACTA CGAGACGGTG
    GCGGGCGGTG CGGGCGCGGG ACCGGGTTGG CACGGGCGCA GCGGCGTGCA CAGCCACATG
    ACCAACACGC GCATCACCGA CCCCGAGATC CTGGAGAGCA GGTACCCGGT TATCCTGCGC
    CGCTTCGAGC TAAGGCTGGG GTCCGGGGGT CGCGGCCGCT TCCGGGGCGG CGATGGCATT
    ATCCGTGAGC TGCTTTTCCG CGAGGAGGCG CTGCTGTCCG TGCTGACTGA GCGCCGCGCC
    TTCCAGCCGT ATGGCCTGAT GGGGGGTGAG CCTGGTGCCC GGGGCCTAAA CCTACTCATC
    CGGAAGGACG GCCGGACAGT AAACCTGGGT GGGAAGACCT CGGTGCCCGT GTACCCCGGG
    GACGTGTTCT GTCTGCACAC ACCAGGCGGT GGGGGCTACG GGGACCCGGA AGACCCCGCC
    CCACTGCCAG GTTCGCCCTT GCAGCCCCTA GCCTTCCCGG AGAGGGGCAG CGTGTATGAG
    TACCGCAGGG CCCAGGAGGC TGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45766
    Clone ID Related Accession (Same CDS sequence) XM_010811822.2 , XM_010811822.3
    Accession Version XM_010811822.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104381.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010811822.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810124.1
    CDS159..3653
    Translation

    Target ORF information:

    RefSeq Version XM_010811822.2
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase (ATP-hydrolysing) (OPLAH), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811822.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45766
    Clone ID Related Accession (Same CDS sequence) XM_010811822.2 , XM_010811822.3
    Accession Version XM_010811822.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3495bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product 5-oxoprolinase isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037341.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010811822.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010810124.1
    CDS165..3659
    Translation

    Target ORF information:

    RefSeq Version XM_010811822.3
    Organism Bos taurus(cattle)
    Definition Bos taurus 5-oxoprolinase, ATP-hydrolysing (OPLAH), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010811822.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCCCGAGA CGCTGTACGA GGAGGTGCTG GAAGTGGACG AACGGGTGGT GCTCTATCGA 
    GGGGAGCCGG GTGCTGGGAC ACCCGTGAAA GGCTGCACAG GGGACCTACT GGAAGTGCAG
    CAGCCTGTGG ACCTGGGGGG CCTGCGAGGG AAGCTGGAAG GTCTCCTGTC CCGGGGCATC
    CGTAGCTTGG CCGTGGTGCT CATGCACTCG TACACGTGGG CCCAGCACGA GCAGCAGGTG
    GGCGCCCTGG CCCGGGAACT GGGCTTCACG CATGTGTCGC TGTCCTCGGA GGCCATGCCC
    ATGGTGCGCA TTGTGCCCCG GGGGCACACG GCCTGTGCTG ACGCCTATCT CACGCCCACC
    ATCCAGCGCT ACGTGCAGGG CTTCCGACGT GGCTTCCAGG GTCAGCTCAA GGACGTGCAG
    GTGCTGTTCA TGCGCTCCGA CGGTGGGCTG GCGCCCATGG ACTCCTTCAG CGGCTCCCGC
    GCTGTGCTCT CCGGCCCTGC GGGGGGCGTG GTTGGTTACT CAGCCACCAC CTACCGGGTG
    GAGGGCGGCC AGCCAGTCAT CGGCTTTGAC ATGGGAGGCA CGTCTACCGA CGTGAGCCGC
    TATGCTGGCG AGTTTGAGCA TGTTTTCGAG GCCAGCACGG CTGGTGTCAC CCTCCAGGCC
    CCCCAGCTGG ATATCAACAC TGTGGCAGCC GGTGGGGGCT CCCGCCTCTT TTTCAGGTCG
    GGCCTCTTCG TGGTGGGGCC AGAGTCTGCG GGAGCCCACC CTGGCCCCGC CTGCTACCGA
    AAAGGGGGCC CTGTGACAGT GACGGATGCT AATCTGGTCC TGGGTCGCCT GCTGCCTGCC
    TCCTTCCCCT GCATTTTTGG GCCGGGAGAG GACCAGCCAC TGTCCCCGGA GGCGTCCCGA
    AAGGCCCTGG AGGCTGTGGC CACGGAGGTC AACAGCTTCC TGACCAATGG GCCTTGCCCG
    GCCTCCCCGC TGAGCCTGGA GGAGGTGGCC ATGGGGTTTG TGCGTGTGGC CAACGAGGCC
    ATGTGTCGGC CCATCCGTGC ACTCACGCAG GCACGAGGTC ATGACCCCTC GGCCCACGTG
    CTGGCCTGCT TTGGGGGAGC TGGTGGGCAG CATGCTTGTG CCATTGCCCG GGCCCTGGGC
    ATGGACACTG TGCACATTCA CAGGCACAGT GGGCTGCTGT CGGCGCTGGG GCTGGCCCTG
    GCTGATGTGG TGCATGAGGC GCAGGAGCCC TGCTCCCTGC CATATGCTCC CGAGACCTTT
    GCGCAGCTGG ACCAGAGGCT GGGCCGCCTG GAGGAGCAGT GTGTGGAGGC CCTGCGGGCC
    CAGGGCTTCC CCAGGTCCCA GATCAGCACC GAGAGCTTCC TGCACCTGCG CTACCAGGGC
    ACGGACTGCG CCCTGATGGT GTCTGCCCAC CAGCACCCGG CCAGTGCCCG CTCACCCCGA
    GCTGGTGACT TTGGGGCAGC CTTTGTGGAG AGGTACATGA GGGAGTTCGG CTTCATTATT
    CCCGAGCGGC CGGTGGTGGT GGATGACGTG CGGGTCAGGG GCACCGGCAG CAGCAGCCTT
    CGCCTCGAGG ACGTCCCAAA AGCCCATAGT GGGCCTCCCC GGGTAGATAA GATGACCCAG
    TGCTACTTTG AGGGGGGCTA CCAGGAGACC CCTGTGTACC TGTTGGGAGA GCTGGGCTGT
    GGGCACAAGC TTCAGGGGCC CTGCCTCATC ATTGACAGCA ACAGTACCAT CCTGGTGGAG
    CCAGGCTGCC AGGCAGAGGT GACTGAGACT GGGGACATCC GCATCTCTGT GGGGGCTGAG
    ACAGCCAGCG TGGTGGGCAC GCAGCTTGAC CCCATCCACC TTTCCATCTT CTCCCACCGC
    TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTGCAGC GCACGGCCAT CTCCACCAAC
    ATCAAGGAGC GTCTGGACTT CTCCTGCGCC CTCTTTGGGC CCGACGGGGG GCTGGTCTCC
    AACGCCCCTC ACATCCCTGT GCACCTGGGT GCCATGCAGG AGACGGTGCA GTTCCAGATT
    CAGCAGTTGG GGGCTGATCT CCACCCCGGC GATGTGCTGC TGAGCAACCA CCCCAGCGCG
    GGGGGCAGCC ACTTGCCAGA CCTGACTGTC ATCACACCGG TGTTTTGGCC GGGTCAGACA
    CGGCCTGTGT TCTATGTGGC CAGCCGTGGG CACCATGCAG ACATTGGGGG CATCACACCA
    GGCTCCATGC CCCCCCACTC CACCAGCCTG CAGCAGGAGG GCGCGGTCTT CCTTTCCTTC
    AAACTTGTGC ATGGGGGAGT CTTCCAGGAA GAGGCGGTGA CTGAAGCCCT GCGGGCGCCA
    GGCAAGATTC CAGGCTGCAG CGGGACACGG AACCTGCACG ACAACCTGTC GGATCTGCGC
    GCCCAGGTGG CGGCCAACCA GAAGGGCATC CAGCTGGTGG GCGAGCTCAT CGGGCAGTAC
    GGCCTGGACG TGGTGCAGGC CTACATGGGC CACATTCAGG CAAACGCGGA GCTCGCTGTG
    AGGGACATGC TCCGGGCCTT TGGAACTGCC CGGCAGGCCC GGGGCCTGCC CCTGGAGGTG
    TCTGCAGAGG ACCACATGGA CGACGGTTCC CCTATCCGAC TCCGAGTGCA GATCAACATG
    AGTCAGGGTA GCGCGGTGTT TGATTTCAGC GGCTCAGGGC CAGAGGTGTT CGGCAACCTC
    AACGCGCCGC GGGCCATCAC GCTGTCTGCG CTCATCTACT GTCTTCGCTG TCTGGTTGGC
    CGCGACATTC CACTCAACCA GGGCTGCCTG GCTCCGGTGC GCGTGGTGAT TCCTAAAGGC
    TCCATCCTAG ACCCTTCCCC CGACGCGGCC GTGGTGGGCG GCAACGTGCT CACGTCGCAG
    CGCGTGGTGG ACGTCATCCT GGGGGCCTTC GGGGCCTGCG CCGCTTCCCA GGGCTGCATG
    AACAACGTGA CCTTGGGCAA CGCCCACATG GGCTACTACG AGACGGTGGC GGGCGGTGCG
    GGCGCGGGAC CGGGTTGGCA CGGGCGCAGC GGCGTGCACA GCCACATGAC CAACACGCGC
    ATCACCGACC CCGAGATCCT GGAGAGCAGG TACCCGGTTA TCCTGCGCCG CTTCGAGCTA
    AGGCTGGGGT CCGGGGGTCG CGGCCGCTTC CGGGGCGGCG ATGGCATTAT CCGTGAGCTG
    CTTTTCCGCG AGGAGGCGCT GCTGTCCGTG CTGACTGAGC GCCGCGCCTT CCAGCCGTAT
    GGCCTGATGG GGGGTGAGCC TGGTGCCCGG GGCCTAAACC TACTCATCCG GAAGGACGGC
    CGGACAGTAA ACCTGGGTGG GAAGACCTCG GTGCCCGTGT ACCCCGGGGA CGTGTTCTGT
    CTGCACACAC CAGGCGGTGG GGGCTACGGG GACCCGGAAG ACCCCGCCCC ACTGCCAGGT
    TCGCCCTTGC AGCCCCTAGC CTTCCCGGAG AGGGGCAGCG TGTATGAGTA CCGCAGGGCC
    CAGGAGGCTG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.