CUL1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	CUL1
Entrez Gene ID	407228
Full Name	cullin 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024990518.1	XP_024846286.1	cullin-1 isoform X1
NM_001193233.1	NP_001180162.1	cullin-1
XM_024990516.1	XP_024846284.1	cullin-1 isoform X1
XM_024990517.1	XP_024846285.1	cullin-1 isoform X1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cul1	NP_036172.1
Danio rerio (zebrafish)	cul1b	NP_998660.1
Neurospora crassa	NCU05204	XP_962203.2
Xenopus tropicalis (tropical clawed frog)	cul1	NP_001039255.1
	CUL1	XP_002803558.1
Canis lupus familiaris (dog)	CUL1	XP_848402.1
Bos taurus (cattle)	CUL1	NP_001180162.1
Schizosaccharomyces pombe (fission yeast)	cul1	NP_594259.1
Magnaporthe oryzae (rice blast fungus)	MGG_07145	XP_003715342.1
Rattus norvegicus (Norway rat)	Cul1	NP_001102097.1
Gallus gallus (chicken)	CUL1	XP_418878.3
Drosophila melanogaster (fruit fly)	lin19	NP_724622.1
Pan troglodytes (chimpanzee)	CUL1	NP_001233533.1
Caenorhabditis elegans (roundworm)	cul-1	NP_499309.1
Homo sapiens (human)	CUL1	NP_003583.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008007	XP_317457.4

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of SCF-Complex during Bovine Preimplantation Development.
Benesova V, Kinterova V, Kanka J, Toralova T
PloS one11(1)e0147096(2016)

Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition.
Kepkova KV, Vodicka P, Toralova T, Lopatarova M, Cech S, Dolezel R, Havlicek V, Besenfelder U, Kuzmany A, Sirard MA, Laurincik J, Kanka J
Theriogenology75(9)1582-95(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The SCF-complex gene expression has been characterized during bovine preimplantation development.
Title: Characterization of SCF-Complex during Bovine Preimplantation Development.

Around the time of major gene activation, expression of cullin 1 switches from transcript variant 1 to transcript variant 3.
Title: Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition.

The following CUL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CUL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa36595	NM_001193233.1 Latest version!	Bos taurus cullin 1 (CUL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36595	XM_024990518.1 Latest version!	Bos taurus cullin 1 (CUL1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36595	XM_024990516.1 Latest version!	Bos taurus cullin 1 (CUL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa36595	XM_024990517.1 Latest version!	Bos taurus cullin 1 (CUL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CUL1 ( NM_001193233.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180162.1 Bos taurus cullin 1 (CUL1), mRNA.

Price & Availability↑

CloneID	OBa36595
Clone ID Related Accession (Same CDS sequence)	XM_024990518.1 , NM_001193233.1 , XM_024990516.1 , XM_024990517.1
Accession Version	NM_001193233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-24
Organism	Bos taurus(cattle)
Product	cullin-1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV885042.1, DV882578.1, EE364210.1, EH201830.1, DN545978.1 and DY068000.1. On Apr 12, 2011 this sequence version replaced XM_876699.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180162.1
CDS	260..2590
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYAVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	NM_001193233.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cullin 1 (CUL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193233.1

ORF Insert Sequence:

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( XM_024990518.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846286.1 Bos taurus cullin 1 (CUL1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa36595
Clone ID Related Accession (Same CDS sequence)	XM_024990518.1 , NM_001193233.1 , XM_024990516.1 , XM_024990517.1
Accession Version	XM_024990518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846286.1
CDS	260..2590
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYAVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_024990518.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cullin 1 (CUL1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990518.1

ORF Insert Sequence:

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( XM_024990516.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846284.1 Bos taurus cullin 1 (CUL1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa36595
Clone ID Related Accession (Same CDS sequence)	XM_024990518.1 , NM_001193233.1 , XM_024990516.1 , XM_024990517.1
Accession Version	XM_024990516.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846284.1
CDS	328..2658
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYAVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_024990516.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cullin 1 (CUL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990516.1

ORF Insert Sequence:

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CUL1 ( XM_024990517.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024846285.1 Bos taurus cullin 1 (CUL1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa36595
Clone ID Related Accession (Same CDS sequence)	XM_024990518.1 , NM_001193233.1 , XM_024990516.1 , XM_024990517.1
Accession Version	XM_024990517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	cullin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037331.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024846285.1
CDS	188..2518
Translation	MSSNRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYAVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

Target ORF information:

RefSeq Version	XM_024990517.1
Organism	Bos taurus(cattle)
Definition	Bos taurus cullin 1 (CUL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024990517.1

ORF Insert Sequence:

ATGTCATCCA ACCGGAGTCA GAACCCCCAC GGACTCAAGC AGATCGGCCT CGACCAGATC 
TGGGACGACC TCAGAGCTGG CATCCAGCAA GTGTACACCA GACAGAGCAT GGCCAAGTCC 
AGATACATGG AACTCTACAC TCACGTTTAT AACTACTGCA CAAGTGTTCA CCAGTCAAAC 
CAAGCTCGAG GGGCTGGTGT CCCTCCTTCT AAATCGAAAA AGGGGCAGAC GCCGGGGGGA 
GCTCAGTTTG TGGGCTTGGA ATTGTACAAA CGGCTTAAAG AGTTCCTGAA GAATTACTTG 
ACAAATCTTC TTAAGGACGG AGAAGATTTG ATGGATGAGA GTGTACTGAA GTTCTACACT 
CAGCAATGGG AAGACTACCG GTTTTCAAGC AAAGTGCTGA ACGGGATCTG TGCCTACCTC 
AATAGACATT GGGTTCGCCG CGAGTGTGAC GAAGGACGGA AGGGAATCTA TGAAATCTAT 
TCTCTTGCAT TGGTGACTTG GAGAGACTGT CTTTTCAGGC CACTGAATAA ACAGGTAACA 
AATGCTGTTT TAAAACTTAT TGAAAAAGAA AGAAATGGTG AAACTATCAA TACAAGACTG 
ATAAGTGGAG TTGTACAGTC TTATGTGGAG CTGGGACTGA ATGAAGATGA CGCCTTTGCA 
AAGGGCCCTA CGCTAACAGT GTACAAAGAA TCCTTTGAAT CTCAGTTTCT GGCTGACACA 
GAAAGATTTT ATACCAGAGA GAGTACCGAA TTCTTGCAGC AGAACCCGGT TACTGAATAT 
ATGAAAAAGG CGGAGGCTCG TCTGCTGGAG GAGCAGCGCA GGGTCCAGGT GTACCTTCAC 
GAGAGCACGC AGGATGAGCT CGCCAGGAAG TGCGAGCAGG TGCTCATCGA GAAACACCTG 
GAGATCTTCC ACACGGAGTT CCAGAACCTG CTGGACGCTG ACAAAAATGA GGATTTGGGT 
CGCATGTATA ATCTTGTATC TAGAATCCAA GATGGCCTGG GAGAATTGAA AAAACTCCTG 
GAGACACACA TTCATAATCA GGGTCTTGCA GCAATTGAAA AGTGTGGAGA AGCTGCATTA 
AATGACCCCA AAATGTATGT ACAGACAGTG TTGGATGTGC ATAAAAAATA CAATGCCCTG 
GTGATGTCGG CGTTCAACAA TGATGCTGGC TTCGTGGCCG CGCTGGACAA GGCTTGCGGT 
CGCTTCATAA ACAACAACGC AGTTACCAAA ATGGCTCAGT CGTCTAGTAA ATCCCCCGAG 
TTGCTGGCTC GATACTGTGA CTCCCTGTTG AAGAAGAGTT CCAAGAACCC AGAAGAAGCA 
GAACTCGAAG ACACACTCAA CCAAGTGATG GTGGTCTTCA AATACATCGA GGACAAGGAC 
GTGTTTCAGA AGTTCTATGC AAAAATGTTG GCCAAGAGGC TCGTGCACCA GAACAGCGCG 
AGCGATGACG CCGAGGCGAG CATGATCTCC AAGCTGAAGC AAGCTTGTGG TTTTGAGTAT 
ACCTCTAAAC TTCAGCGGAT GTTTCAAGAC ATTGGTGTAA GCAAAGATTT GAACGAGCAG 
TTCAAAAAGC ACCTAACGAA CTCCGAGCCA CTTGACTTGG ATTTCAGTAT TCAAGTGCTG 
AGTTCCGGAT CGTGGCCCTT TCAGCAGTCT TGCACGTTTG CCTTGCCGTC GGAGCTGGAA 
CGGAGTTACC AGCGATTCAC AGCCTTTTAC GCCAGCCGTC ACAGTGGCAG AAAATTGACG 
TGGTTATATC AACTGTCAAA AGGAGAATTA GTAACAAACT GCTTCAAAAA CAGATACACT 
TTGCAGGCAT CCACATTCCA GATGGCCATC CTGCTCCAGT ATAACACAGA GGACGCCTAT 
GCCGTGCAGC AGCTGACGGA CAGCACCCAG ATCAAAATGG ACATTTTGGC ACAAGTCCTA 
CAGATTTTAT TGAAGTCCAA GTTATTGGTC TTGGAAGATG AAAACGCAAA TGTTGATGAG 
GTGGAATTGA AGCCAGATAC CTTAATAAAA TTATATCTTG GTTATAAAAA TAAAAAATTA 
AGGGTTAACA TCAATGTGCC CATGAAAACG GAACAGAAGC AGGAACAGGA AACCACACAC 
AAAAACATCG AGGAAGATCG AAAACTACTG ATCCAGGCGG CTATTGTGAG AATTATGAAA 
ATGAGGAAGG TCCTAAAACA CCAGCAGTTA CTTGGAGAAG TCCTGACTCA GCTGTCCTCA 
AGATTCAAGC CCCGGGTCCC AGTAATTAAG AAATGCATTG ACATTCTGAT CGAGAAGGAG 
TATCTGGAGC GAGTGGACGG GGAGAAGGAC ACCTACAGCT ACCTGGCCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of SCF-Complex during Bovine Preimplantation Development.
PloS one11(1)e0147096(2016)
Benesova V,Kinterova V,Kanka J,Toralova T

Transcriptomic analysis of in vivo and in vitro produced bovine embryos revealed a developmental change in cullin 1 expression during maternal-to-embryonic transition.
Theriogenology75(9)1582-95(2011 Jun)
Kepkova KV,Vodicka P,Toralova T,Lopatarova M,Cech S,Dolezel R,Havlicek V,Besenfelder U,Kuzmany A,Sirard MA,Laurincik J,Kanka J

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CUL1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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