SLC12A1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005211900.4	XP_005211957.2	solute carrier family 12 member 1 isoform X2
XM_015473209.1	XP_015328695.1	solute carrier family 12 member 1 isoform X1
XM_005211900.3	XP_005211957.1	solute carrier family 12 member 1 isoform X2
XM_002690936.6	XP_002690982.2	solute carrier family 12 member 1 isoform X3
XM_015465137.1	XP_015320623.1	solute carrier family 12 member 1 isoform X3
XM_002700626.5	XP_002700672.2	solute carrier family 12 member 1 isoform X2
XM_005211899.3	XP_005211956.1	solute carrier family 12 member 1 isoform X1
XM_005211899.4	XP_005211956.2	solute carrier family 12 member 1 isoform X1
XM_015465135.1	XP_015320621.1	solute carrier family 12 member 1 isoform X1
XM_002690936.5	XP_002690982.1	solute carrier family 12 member 1 isoform X3
XM_015473209.2	XP_015328695.2	solute carrier family 12 member 1 isoform X1
XM_015465136.1	XP_015320622.1	solute carrier family 12 member 1 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa219909	XM_015473209.2 Latest version!	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219909	XM_005211899.4 Latest version!	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219910	XM_005211900.4 Latest version!	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa219911	XM_002690936.6 Latest version!	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OBa06571	XM_005211900.3 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa06571	XM_002700626.5 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23414	XM_015465135.1 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23414	XM_005211899.3 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23414	XM_015465136.1 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23414	XM_015473209.1 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23415	XM_015465137.1 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa23415	XM_002690936.5 Latest version!	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

SLC12A1 ( XM_005211899.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211956.2 Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa219909
Clone ID Related Accession (Same CDS sequence)	XM_005211899.4 , XM_015473209.2
Accession Version	XM_005211899.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211899.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCAA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211956.2
CDS	219..3614
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005211899.4
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211899.4

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCAA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_015473209.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328695.2 Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa219909
Clone ID Related Accession (Same CDS sequence)	XM_005211899.4 , XM_015473209.2
Accession Version	XM_015473209.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015473209.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCAA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328695.2
CDS	238..3633
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_015473209.2
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473209.2

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCAA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005211900.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211957.2 Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa219910
Clone ID Related Accession (Same CDS sequence)	XM_005211900.4
Accession Version	XM_005211900.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005211900.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211957.2
CDS	219..3518
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005211900.4
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211900.4

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_002690936.6 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690982.2 Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa219911
Clone ID Related Accession (Same CDS sequence)	XM_002690936.6
Accession Version	XM_002690936.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037337.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002690936.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690982.2
CDS	219..3518
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVQEELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_002690936.6
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 member 1 (SLC12A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690936.6

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCAAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005211900.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211957.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

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CloneID	OBa06571
Clone ID Related Accession (Same CDS sequence)	XM_005211900.3 , XM_002700626.5
Accession Version	XM_005211900.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211900.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211957.1
CDS	243..3542
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005211900.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211900.3

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_002700626.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002700672.2 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa06571
Clone ID Related Accession (Same CDS sequence)	XM_005211900.3 , XM_002700626.5
Accession Version	XM_002700626.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002700626.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002700672.2
CDS	243..3542
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_002700626.5
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002700626.5

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTCA TCATCATCGG CCTGAGTGTG 
GTAGTGACAA CACTTACAGG TATTTCTATG TCCGCTATTT GCACAAATGG AGTGGTAAGA 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_015473209.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015328695.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23414
Clone ID Related Accession (Same CDS sequence)	XM_015473209.1 , XM_005211899.3 , XM_015465135.1 , XM_015465136.1
Accession Version	XM_015473209.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015328695.1
CDS	260..3655
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_015473209.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015473209.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_005211899.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005211956.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa23414
Clone ID Related Accession (Same CDS sequence)	XM_015473209.1 , XM_005211899.3 , XM_015465135.1 , XM_015465136.1
Accession Version	XM_005211899.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005211899.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005211956.1
CDS	243..3638
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_005211899.3
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005211899.3

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_015465135.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320621.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa23414
Clone ID Related Accession (Same CDS sequence)	XM_015473209.1 , XM_005211899.3 , XM_015465135.1 , XM_015465136.1
Accession Version	XM_015465135.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320621.1
CDS	260..3655
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_015465135.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465135.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_015465136.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320622.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

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CloneID	OBa23414
Clone ID Related Accession (Same CDS sequence)	XM_015473209.1 , XM_005211899.3 , XM_015465135.1 , XM_015465136.1
Accession Version	XM_015465136.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320622.1
CDS	243..3638
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGLGVIIIGLSVVVTTLTGISMSAICTNGVVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_015465136.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465136.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTCTTG GAGTCATCAT CATCGGCCTG AGTGTGGTAG TGACAACACT TACAGGTATT 
TCTATGTCCG CTATTTGCAC AAATGGAGTG GTAAGAGGAG GTGGGGCCTA CTATCTTATT 
TCCAGAAGCT TAGGGCCTGA GTTCGGTGGA TCTATAGGCT TGATCTTTGC TTTTGCCAAT 
GCAGTGGCTG TTGCTATGTA TGTGGTGGGA TTTGCTGAGA CTGTGGTAGA TCTTCTTAAG 
GAGACTGATT CGATGATGGT GGATCCAACC AATGACATCC GGATCATAGG CTCCATCACA 
GTGGTGATTC TTTTAGGAAT TTCAGTAGCT GGAATGGAAT GGGAAGCAAA GGCTCAAGTG 
ATTCTTCTGA TCATTCTTCT CATTGCTATT GCAAACTTCT TCATTGGAAC TGTCATTCCA 
TCCAACAATG AGAAAAGGGC CAGAGGTTTC TTTAATTACC AAGCATCAAT ATTTGCAGAA 
AATTTTGGGC CAAGCTTCAC AAAAGGTGAA GGCTTCTTCT CTGTCTTTGC CATTTTTTTC 
CCAGCAGCAA CTGGGATCCT TGCTGGTGCC AATATCTCGG GAGATTTGGA GGATCCCCAA 
GATGCCATCC CCAAAGGAAC CATGCTGGCC ATCTTCATCA CCACTGTCGC CTACTTAGGA 
GTCGCCATTT GCGTGGGGGC TTGTGTGGTC CGAGATGCCA CTGGGAGCGT GAATGACACC 
ATCATTTCTG GGATGAACTG CAATGGTTCA GCAGCCTGTG GGTTAGGCTA CGACTTCTCA 
AGATGTCGAC ATGAACCATG TCAGTATGGG CTGATGAACA ATTTCCAGGT CATGAGCATG 
GTGTCAGGGT TTGGCCCCCT CATCACTGCT GGGATCTTTT CTGCAACACT CTCCTCGGCC 
CTGGCCTCCC TCGTCAGCGC GCCCAAAGTG TTCCAGGCTC TGTGCAAGGA CAACATCTAC 
AAAGCTCTGC AGTTCTTTGC AAAGGGATAT GGGAAGAACA ATGAGCCCCT TAGAGGATAC 
TTTCTCACTT TTGTTATAGC CATGGCGTTC ATTCTTATTG CGGAACTGAA CACCATCGCT 
CCCATCATCT CCAATTTTTT CCTGGCCTCA TATGCACTTA TTAATTTCTC CTGCTTTCAT 
GCCTCTTATG CCAAATCTCC AGGATGGAGA CCTGCATATG GTATTTACAA CATGTGGGTG 
TCTCTCTTTG GAGCCGTTTT GTGCTGTGCG GTCATGTTTG TCATCAACTG GTGGGCAGCT 
GTCATCACCT ACGTCATTGA GTTCTTCCTC TATATATACG TGACTTACAA GAAGCCAGAT 
GTGAACTGGG GCTCCTCCAC GCAGGCTCTT TCCTACATGA GCGCTTTAGA CAATGCTCTA 
GAACTAACCA CAGTGGAAGA CCATGTGAAA AACTTCAGGC CCCAGTGCAT TGTTTTAACA 
GGGGGACCCA TGACAAGACC TGCTCTCTTG GATATAACTC ATGCCTTTAC CAAGAACAGT 
GGCCTTTGCA TCTGCTGTGA AGTCTTTGTG GGACCACGCA AGCTGTGTGT TAAAGAGATG 
AACAGTGGCA TGGCAAAAAA GCAGGCCTGG CTTATAAAGA ACAAAATCAA GGCGTTTTAT 
GCAGCAGTGG CAGCAGATTG TTTCAGGGAT GGTGTCCGAA GTCTCCTTCA GGCCTCGGGC 
TTAGGAAGAA TGAAACCAAA CACTCTGGTG ATTGGATATA AAAAAAACTG GAGGAAGGCT 
CCCCTGACAG AGATTGAAAA CTATGTGGGA ATCATACACG ATGCATTTGA TTTTGAGATT 
GGTGTTGTCA TAGTCAGAAT CAGCCAAGGG TTTGACATCT CCCAGGTTCT TCAGGTGCGA 
GAGGAATTAG AGAAATTAGA ACAGGAGCGG CTGGCTTTGG AAGCCACCAT CAAAGACAAT 
GAAAGTGAAG AAGGAAATGG AGGCATCCGA GGCTTGTTTA AAAAAGCTGG CAAGCTGAAC 
ATTACTAAGC CAACTCCTAA GAAAGATAGC AGCATTAACA CAATCCAGTC AATGCACGTT 
GGGGAGTTCA ACCAGAAACT GGTGGAGGCC AGCACCCAAT TTAAAAAGAA GCAGGGAAAA 
GGCACAATTG ATGTTTGGTG GTTGTTTGAT GATGGAGGGT TAATACTCCT TATCCCCTAT 
ATCTTGACTC TCAGAAAAAA ATGGAAAGAC TGTAAATTAA GAATCTATGT TGGAGGGAAG 
ATCAATCGCA TTGAAGAAGA AAAAATTGCA ATGGCTTCCC TTCTGAGCAA ATTTAGGATA 
AAATTTGCAG ACATCCATGT CATTGGTGAC ATCAACGTTA AGCCAAACAA AGAAAGCTGG 
AAAGTCTTTG AAGAAATGAT TGAACCATAT TGTCTCCATG AAAGCTGCAA AGATTTAACA 
ACTGCTGAGA AATTAAAGAG AGAAACTCCG TGGAAAATTA CAGATGCAGA ACTGGAGGCA 
GTCAAGGAAA AGAGTTACCG CCAAGTTCGA CTGAACGAAC TCTTACAGGA GCACTCAAGA 
GCTGCTAATC TCATAGTCCT GAGCCTTCCA GTGGCAAGAA AAGGATCTAT ATCAGATTGG 
TTGTACATGG CTTGGTTGGA AATCCTCACG AAGAACCTCC CTCCTGTCTT ACTAGTTAGA 
GGAAATCACA AAAATGTCCT GACATTTTAC TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_015465137.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015320623.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa23415
Clone ID Related Accession (Same CDS sequence)	XM_015465137.1 , XM_002690936.5
Accession Version	XM_015465137.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014645882.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015320623.1
CDS	243..3542
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_015465137.1
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015465137.1

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A1 ( XM_002690936.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002690982.1 Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa23415
Clone ID Related Accession (Same CDS sequence)	XM_015465137.1 , XM_002690936.5
Accession Version	XM_002690936.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	solute carrier family 12 member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104282.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002690936.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002690982.1
CDS	243..3542
Translation	MSLNNSSNVFLDSTPSNTNRFQVNVINESHESSAAMNDNADPPHYEETSFGDEGQNRFRISFRPGNQECYDNFLQTGETAKTDASFHAYDSHTNTYYLQTFGHNTVDAVPKIEYYRNTGSVSGPKVNRPSLLDIHEQLAKNVSVAPGSADVVANGEGTPGDEQAENKGEDQAGAVKFGWVKGVLVRCMLNIWGVMLFIRLSWIVGEAGIGLGVLIILLSTMVTSITGLSTSAIATNGFVRGGGAYYLISRSLGPEFGGSIGLIFAFANAVAVAMYVVGFAETVVDLLKETDSMMVDPTNDIRIIGSITVVILLGISVAGMEWEAKAQVILLIILLIAIANFFIGTVIPSNNEKRARGFFNYQASIFAENFGPSFTKGEGFFSVFAIFFPAATGILAGANISGDLEDPQDAIPKGTMLAIFITTVAYLGVAICVGACVVRDATGSVNDTIISGMNCNGSAACGLGYDFSRCRHEPCQYGLMNNFQVMSMVSGFGPLITAGIFSATLSSALASLVSAPKVFQALCKDNIYKALQFFAKGYGKNNEPLRGYFLTFVIAMAFILIAELNTIAPIISNFFLASYALINFSCFHASYAKSPGWRPAYGIYNMWVSLFGAVLCCAVMFVINWWAAVITYVIEFFLYIYVTYKKPDVNWGSSTQALSYMSALDNALELTTVEDHVKNFRPQCIVLTGGPMTRPALLDITHAFTKNSGLCICCEVFVGPRKLCVKEMNSGMAKKQAWLIKNKIKAFYAAVAADCFRDGVRSLLQASGLGRMKPNTLVIGYKKNWRKAPLTEIENYVGIIHDAFDFEIGVVIVRISQGFDISQVLQVREELEKLEQERLALEATIKDNESEEGNGGIRGLFKKAGKLNITKPTPKKDSSINTIQSMHVGEFNQKLVEASTQFKKKQGKGTIDVWWLFDDGGLILLIPYILTLRKKWKDCKLRIYVGGKINRIEEEKIAMASLLSKFRIKFADIHVIGDINVKPNKESWKVFEEMIEPYCLHESCKDLTTAEKLKRETPWKITDAELEAVKEKSYRQVRLNELLQEHSRAANLIVLSLPVARKGSISDWLYMAWLEILTKNLPPVLLVRGNHKNVLTFYS

Target ORF information:

RefSeq Version	XM_002690936.5
Organism	Bos taurus(cattle)
Definition	Bos taurus solute carrier family 12 (sodium/potassium/chloride transporter), member 1 (SLC12A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002690936.5

ORF Insert Sequence:

ATGTCACTGA ACAACTCTTC CAATGTATTT CTGGATTCCA CGCCCAGTAA TACCAATCGC 
TTTCAAGTTA ATGTCATAAA TGAGAGCCAT GAGAGCAGTG CGGCTATGAA TGACAATGCT 
GATCCTCCAC ATTATGAGGA AACCTCTTTT GGGGATGAAG GTCAGAACAG ATTTAGAATC 
AGCTTTAGGC CTGGGAATCA GGAGTGCTAC GACAATTTCC TCCAAACTGG AGAAACGGCT 
AAAACAGATG CCAGTTTTCA CGCTTACGAT TCTCACACAA ACACATACTA CCTACAAACC 
TTTGGCCACA ACACTGTGGA TGCTGTGCCC AAGATCGAGT ACTATCGCAA CACCGGCAGT 
GTTAGCGGGC CCAAGGTCAA CCGACCCAGC CTGCTGGACA TTCACGAGCA ACTTGCAAAG 
AATGTATCAG TGGCCCCAGG TTCAGCTGAT GTGGTTGCTA ACGGTGAAGG GACACCTGGA 
GATGAACAAG CTGAAAACAA GGGAGAAGAT CAAGCTGGCG CTGTGAAGTT TGGATGGGTG 
AAAGGTGTGC TGGTACGATG TATGCTGAAT ATCTGGGGTG TCATGCTCTT CATTCGCCTC 
TCCTGGATTG TTGGAGAAGC TGGAATCGGT CTTGGAGTTC TCATAATTCT TCTTTCCACC 
ATGGTCACTT CTATTACTGG GTTGTCAACT TCTGCAATAG CAACTAACGG GTTTGTTCGT 
GGAGGTGGGG CCTACTATCT TATTTCCAGA AGCTTAGGGC CTGAGTTCGG TGGATCTATA 
GGCTTGATCT TTGCTTTTGC CAATGCAGTG GCTGTTGCTA TGTATGTGGT GGGATTTGCT 
GAGACTGTGG TAGATCTTCT TAAGGAGACT GATTCGATGA TGGTGGATCC AACCAATGAC 
ATCCGGATCA TAGGCTCCAT CACAGTGGTG ATTCTTTTAG GAATTTCAGT AGCTGGAATG 
GAATGGGAAG CAAAGGCTCA AGTGATTCTT CTGATCATTC TTCTCATTGC TATTGCAAAC 
TTCTTCATTG GAACTGTCAT TCCATCCAAC AATGAGAAAA GGGCCAGAGG TTTCTTTAAT 
TACCAAGCAT CAATATTTGC AGAAAATTTT GGGCCAAGCT TCACAAAAGG TGAAGGCTTC 
TTCTCTGTCT TTGCCATTTT TTTCCCAGCA GCAACTGGGA TCCTTGCTGG TGCCAATATC 
TCGGGAGATT TGGAGGATCC CCAAGATGCC ATCCCCAAAG GAACCATGCT GGCCATCTTC 
ATCACCACTG TCGCCTACTT AGGAGTCGCC ATTTGCGTGG GGGCTTGTGT GGTCCGAGAT 
GCCACTGGGA GCGTGAATGA CACCATCATT TCTGGGATGA ACTGCAATGG TTCAGCAGCC 
TGTGGGTTAG GCTACGACTT CTCAAGATGT CGACATGAAC CATGTCAGTA TGGGCTGATG 
AACAATTTCC AGGTCATGAG CATGGTGTCA GGGTTTGGCC CCCTCATCAC TGCTGGGATC 
TTTTCTGCAA CACTCTCCTC GGCCCTGGCC TCCCTCGTCA GCGCGCCCAA AGTGTTCCAG 
GCTCTGTGCA AGGACAACAT CTACAAAGCT CTGCAGTTCT TTGCAAAGGG ATATGGGAAG 
AACAATGAGC CCCTTAGAGG ATACTTTCTC ACTTTTGTTA TAGCCATGGC GTTCATTCTT 
ATTGCGGAAC TGAACACCAT CGCTCCCATC ATCTCCAATT TTTTCCTGGC CTCATATGCA 
CTTATTAATT TCTCCTGCTT TCATGCCTCT TATGCCAAAT CTCCAGGATG GAGACCTGCA 
TATGGTATTT ACAACATGTG GGTGTCTCTC TTTGGAGCCG TTTTGTGCTG TGCGGTCATG 
TTTGTCATCA ACTGGTGGGC AGCTGTCATC ACCTACGTCA TTGAGTTCTT CCTCTATATA 
TACGTGACTT ACAAGAAGCC AGATGTGAAC TGGGGCTCCT CCACGCAGGC TCTTTCCTAC 
ATGAGCGCTT TAGACAATGC TCTAGAACTA ACCACAGTGG AAGACCATGT GAAAAACTTC 
AGGCCCCAGT GCATTGTTTT AACAGGGGGA CCCATGACAA GACCTGCTCT CTTGGATATA 
ACTCATGCCT TTACCAAGAA CAGTGGCCTT TGCATCTGCT GTGAAGTCTT TGTGGGACCA 
CGCAAGCTGT GTGTTAAAGA GATGAACAGT GGCATGGCAA AAAAGCAGGC CTGGCTTATA 
AAGAACAAAA TCAAGGCGTT TTATGCAGCA GTGGCAGCAG ATTGTTTCAG GGATGGTGTC 
CGAAGTCTCC TTCAGGCCTC GGGCTTAGGA AGAATGAAAC CAAACACTCT GGTGATTGGA 
TATAAAAAAA ACTGGAGGAA GGCTCCCCTG ACAGAGATTG AAAACTATGT GGGAATCATA 
CACGATGCAT TTGATTTTGA GATTGGTGTT GTCATAGTCA GAATCAGCCA AGGGTTTGAC 
ATCTCCCAGG TTCTTCAGGT GCGAGAGGAA TTAGAGAAAT TAGAACAGGA GCGGCTGGCT 
TTGGAAGCCA CCATCAAAGA CAATGAAAGT GAAGAAGGAA ATGGAGGCAT CCGAGGCTTG 
TTTAAAAAAG CTGGCAAGCT GAACATTACT AAGCCAACTC CTAAGAAAGA TAGCAGCATT 
AACACAATCC AGTCAATGCA CGTTGGGGAG TTCAACCAGA AACTGGTGGA GGCCAGCACC 
CAATTTAAAA AGAAGCAGGG AAAAGGCACA ATTGATGTTT GGTGGTTGTT TGATGATGGA 
GGGTTAATAC TCCTTATCCC CTATATCTTG ACTCTCAGAA AAAAATGGAA AGACTGTAAA 
TTAAGAATCT ATGTTGGAGG GAAGATCAAT CGCATTGAAG AAGAAAAAAT TGCAATGGCT 
TCCCTTCTGA GCAAATTTAG GATAAAATTT GCAGACATCC ATGTCATTGG TGACATCAAC 
GTTAAGCCAA ACAAAGAAAG CTGGAAAGTC TTTGAAGAAA TGATTGAACC ATATTGTCTC 
CATGAAAGCT GCAAAGATTT AACAACTGCT GAGAAATTAA AGAGAGAAAC TCCGTGGAAA 
ATTACAGATG CAGAACTGGA GGCAGTCAAG GAAAAGAGTT ACCGCCAAGT TCGACTGAAC 
GAACTCTTAC AGGAGCACTC AAGAGCTGCT AATCTCATAG TCCTGAGCCT TCCAGTGGCA 
AGAAAAGGAT CTATATCAGA TTGGTTGTAC ATGGCTTGGT TGGAAATCCT CACGAAGAAC 
CTCCCTCCTG TCTTACTAGT TAGAGGAAAT CACAAAAATG TCCTGACATT TTACTCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SLC12A1
Entrez Gene ID	407161
Full Name	solute carrier family 12 member 1
Synonyms	BSC1
General protein information	Preferred Names solute carrier family 12 member 1 Names solute carrier family 12 member 1 apical Na(2Cl)K cotransporter solute carrier family 12 (sodium/potassium/chloride transporters), member 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255791.1
Homo sapiens (human)	SLC12A1	NP_000329.2
Gallus gallus (chicken)	SLC12A1	XP_004943856.1
Canis lupus familiaris (dog)	SLC12A1	XP_535467.2
Pan troglodytes (chimpanzee)	SLC12A1	NP_001104309.1
	SLC12A1	XP_001112900.1
Bos taurus (cattle)	SLC12A1	XP_005211957.1
Mus musculus (house mouse)	Slc12a1	NP_899197.2
Rattus norvegicus (Norway rat)	Slc12a1	NP_001257546.1
Caenorhabditis elegans (roundworm)	nkcc-1	NP_001255788.1
Xenopus tropicalis (tropical clawed frog)	slc12a1	XP_004912656.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SLC12A1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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