COL4A4 cDNA ORF clone, Bos taurus(cattle)

The following COL4A4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL4A4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227809 XM_024981732.1
Latest version!
Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227809 XM_024981728.1
Latest version!
Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OBa227810 XM_024981736.1
Latest version!
Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227811 XM_024981740.1
Latest version!
Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa227812 XM_024981745.1
Latest version!
Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OBa88012 XM_015459732.1
Latest version!
Bos taurus collagen type IV alpha 4 (COL4A4), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227809
    Clone ID Related Accession (Same CDS sequence) XM_024981732.1 , XM_024981728.1
    Accession Version XM_024981732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837500.1
    CDS354..5423
    Translation

    Target ORF information:

    RefSeq Version XM_024981732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227809
    Clone ID Related Accession (Same CDS sequence) XM_024981732.1 , XM_024981728.1
    Accession Version XM_024981728.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5070bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837496.1
    CDS244..5313
    Translation

    Target ORF information:

    RefSeq Version XM_024981728.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981728.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227810
    Clone ID Related Accession (Same CDS sequence) XM_024981736.1
    Accession Version XM_024981736.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4953bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGCCCTCC TGGGCCCATG GGGGATCCTG GGCCCATAGG GTTTGGCCCT
    GGATACCTCA GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGATGGAGA ACCCACCTGC
    CCCATGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGC TATACCTGGA AGGACAGGAG
    AGAGCTCACA ATCAAGACCT CGGTCTGGCG GGCTCCTGCC TCCCCATCTT TAGCACGCTG
    CCCTTCGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCGGAGGAA CGACAGGTCC
    TACTGGCTGG CGAGCACAGC GCCTCTGCCC ATGACTCCGC TCTCGGAGGA CGAGATCCGC
    CCGTACATCA GCCGCTGCGC AGTGTGCGAG GCCCCCGCCC AGGCCGTGGC GGTGCACAGC
    CAAGACCAGT CCATCCCCCC ATGTCCGCGG GCCTGGAGGA GCCTCTGGAT CGGGTACTCC
    TTCCTCATGC ACACAGGCGC TGGGGACCAA GGAGGCGGGC AGGCCCTCAT GTCCCCAGGC
    AGCTGCCTAG AGGATTTCAG AGCCGCACCG TTCCTGGAAT GCCAAGGCCG GCAGGGAACT
    TGCCACTTTT TTGCCAACAA GTATAGTTTC TGGCTGACAA CAGTGAGACC TGACTTGCAG
    TTTTCCTCTG CACCATTACC GGACACCTTG AAAGAAAGCC ATGCTCAACG CCAGAAAATC
    AGCAGGTGCC AGGTCTGCGT GAAGCATAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837504.1
    CDS245..5197
    Translation

    Target ORF information:

    RefSeq Version XM_024981736.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981736.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGCCCTCC TGGGCCCATG GGGGATCCTG GGCCCATAGG GTTTGGCCCT
    GGATACCTCA GTGGCTTCCT CCTGGTTCTC CACAGTCAGA CGGATGGAGA ACCCACCTGC
    CCCATGGGCA TGCCCAGGCT CTGGACTGGG TATAGTCTGC TATACCTGGA AGGACAGGAG
    AGAGCTCACA ATCAAGACCT CGGTCTGGCG GGCTCCTGCC TCCCCATCTT TAGCACGCTG
    CCCTTCGCCT ACTGCAACAT CCACCAGGTG TGCCACTATG CCCGGAGGAA CGACAGGTCC
    TACTGGCTGG CGAGCACAGC GCCTCTGCCC ATGACTCCGC TCTCGGAGGA CGAGATCCGC
    CCGTACATCA GCCGCTGCGC AGTGTGCGAG GCCCCCGCCC AGGCCGTGGC GGTGCACAGC
    CAAGACCAGT CCATCCCCCC ATGTCCGCGG GCCTGGAGGA GCCTCTGGAT CGGGTACTCC
    TTCCTCATGC ACACAGGCGC TGGGGACCAA GGAGGCGGGC AGGCCCTCAT GTCCCCAGGC
    AGCTGCCTAG AGGATTTCAG AGCCGCACCG TTCCTGGAAT GCCAAGGCCG GCAGGGAACT
    TGCCACTTTT TTGCCAACAA GTATAGTTTC TGGCTGACAA CAGTGAGACC TGACTTGCAG
    TTTTCCTCTG CACCATTACC GGACACCTTG AAAGAAAGCC ATGCTCAACG CCAGAAAATC
    AGCAGGTGCC AGGTCTGCGT GAAGCATAGC TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227811
    Clone ID Related Accession (Same CDS sequence) XM_024981740.1
    Accession Version XM_024981740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGATGA GGAAGCTGAG GTTGAGAGAG GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837508.1
    CDS244..5082
    Translation

    Target ORF information:

    RefSeq Version XM_024981740.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGATGA GGAAGCTGAG GTTGAGAGAG GATAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227812
    Clone ID Related Accession (Same CDS sequence) XM_024981745.1
    Accession Version XM_024981745.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGC ACACAGGCGC TGGGGACCAA GGAGGCGGGC AGGCCCTCAT
    GTCCCCAGGC AGCTGCCTAG AGGATTTCAG AGCCGCACCG TTCCTGGAAT GCCAAGGCCG
    GCAGGGAACT TGCCACTTTT TTGCCAACAA GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024837513.1
    CDS243..4898
    Translation

    Target ORF information:

    RefSeq Version XM_024981745.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 chain (COL4A4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGGT
    CAACCGGGAC CAGCAGGGCC ACAGGGTCCT ATTGGACCCC TGGGACCACC GGGACCCACT
    GGGATCCCAG GGGAGAAAGG GATGAAAGGT GACAGTGGCC GTCCTGGAGC AGCAGGTGAC
    GTAGGTGACA AGGGTCCAAC CGGAGTTCCT GGATTTCCAG GTCTGGATGG GATACCTGGG
    CACCCAGGGC CTCCTGGATC CAGAGGCAGA CCTGGCATAG ATGGCTACAA TGGCTCAAGA
    GGTGATCCAG GGATTCCAGG AGAAAGAGGA GCTCTTGGCC CACGAGGGCC CCCAGGCCTT
    CCTGGAGAAA AAGGAGAAAA AGGAAATTCA GTGTTCATTT TAGGTGCCAC TAAAGGTATG
    CAGGGCGACA GAGGGGACCC AGGACCTCCC GGCTCACCAG GATCAAGGGG AGTGAGAGGC
    CCAGCAGGCC CAGTGGGGTA CCCAGGAGAG CCCGGGTTAG CAGGACCTCC AGGCCCCCCT
    GGGAGTCCAG GCTTAAAGGG TAATCCTGGC GTGGGAGTAA AGGGGCAAAT GGGAGACCCG
    GGTGAGGTTG GCCAACAAGG TTCTCCGGGG CCCACTCTGT TGGTGCAGCC ACCTGATTCT
    GGTCTCTATA AAGGAGAAAA GGGTATTAAA GGAATGCCTG GAATGACTGG ACTTCCTGGA
    CCACCGGGAC CCAGGGGAGA GCCTGGTATT GGGAGAAAAG GAGAGAAAGG GATTCCTGGG
    TTTCCAGGAC CTCGGGGGGA CCCTGGTTCC TATGGATCTC CAGGTTTTCC AGGATTAAAG
    GGGGAACCAG GACTGTTTGG AGATCCTGGG TCATTTGGAT TTCTTGGCCC AAAGGGAGAT
    CCTGGAGACC GAGGGCACCC AGGACCACCA GGGGTTTTGG TAACTCCATC TCTTCCACTC
    AAAGGCCCTC CAGGGGATCC AGGGCGCCCT GGCCGCTATG GAGAAACAGG GGCTGTTGGA
    CCACGTGGTC CTCCTGGTGC CCCAGGTAGA CCAGGGGAAG CCTGTCCAGG CATGACGGGA
    CCTCCTGGGC CACGAGGATT TCCTGGTCAC CCAGGATTTC CAGGGGCAGC TGGGGTTCCT
    GGAAGAGCTG ATTCTGCTCC AGGCAAACCA GGCAACCAGG GACCAACTGG GCTGCCTGGA
    GTGCCGGGAC TGCCGGGACC TCCGGGATCA GATGCTGTAT ATTGTACTGT TGGGCAACCC
    GGACCACAAG GAACAAAAGG CAAAGTGGGT CCTCCAGGAA GACGAGGCTC CAAAGGAGAA
    AAAGGGAGCT CAGGGCTCTG TGCCTGCCAG CCTGGTCCCA TGGGCCCATC AGGCCCTCCA
    GGGCCTCCTG GGAGGCAAGG GAGTAAGGGA GACTTGGGGC TCCCTGGGTG GCTTGGAGAA
    AAAGGTTCCC CAGGCCCTCC TGGTGCCAAA GGATCTCCAG GGCCACCGGG AAAACCTGGT
    GCCTCAGGAC TACCTGGCAG CAAAGGAGAA AAAGGTGACA TGGTTGTATC AAGAGTGAAA
    GGACACAAAG GAGAAAGAGG GCCTGATGGG CCCCCTGGAC TTCCAGGGCA GCAGGGACAA
    CACGGTCGAG ATGGATATGC TGGAGAAAAG GGGGACACAG GGCCGCCTGG TGATCACGAG
    GATGCAGCCC CAGGTGATAA AGGGCCTCCT GGACCGCCAG GGCCCCCTGG CAGAGCGGGA
    CCTAGGGGGC CCCCAGGCCT GGGATTTCCT GGCCCTCCAG GAGACAGAGG GCAACCAGGA
    GCTCCAGGCT ACCCAGGCAA CAGGGGTCCT GATGGTCGGA AGGGTCGGAA AGGTGATACA
    ATTCCTTGTA ACGTAACATA CCCTGGGAGG CCAGGCCCTC CAGGTTTTGA TGGTCCTCCA
    GGTCCAAAGG GACTTCCAGG TCATCGGGGT GCTCCTGGGC TGAGGTGTTT GGATGGACAG
    AAGGGTCAAC GTGGCAAACC AGGAATCTCA GAAAGACCTG GTCCACCTGG TTTTCGTGGT
    GATATAGGAG AACCAGGTTT GGGAGGTGAA AAGGGGTCCT CGCCTGTTGG GCCCCCAGGC
    ACTCCAGGTT CACCTGGAGT GAATGGTCAG AAAGGACCCC CAGGAGATCC TGCTATCGGC
    TACCCAGGAC TCCCGGGAAA GAGCGGTCTT CCAGGAGCAC CAGGGTCCAA AGGACTCAGA
    GGCGATGCCG GGCGTCCAGG GACCGCAGGG CCAGCTGGCA TGCCCGGATT TCCGGGTCTC
    CAAGGTCCCA AAGGCCGAGA GGGAAATGCT GGGTTTCCAG GGATCCCAGG CCCACCTGGT
    CACTCCTGTG AAAGAGGTGC TCCAGGGATA CCAGGGCAAC CGGGGCTCCC TGGGGCTCCA
    GGCAGTCCAG GTGCCCCAGG TTGGAAAGGA CAGCGAGGGG ACATGGGACC TCTGGGACCT
    GCTGGAATGA AGGGCCTTCC TGGAGTCCCA GGATGGCCGG GGGCAGATGG AACCCCAGGT
    CTTCCAGGGG TCCCAGGCCC CACTGGAGAC GATGGGCTAC CTGGTCTTCC AGGCCCAAAG
    GGACCCCAGG GGCTGCCTGG CTTCCTGGGT TTTCCAGGAG AGAGAGGAAA ACCTGGTCAG
    GATGGACACC CTGGCAGAAA GGGAGAACAT GGAGAGAAGG GTTGGCCTGG CTTCCTGGGA
    GACCGAGGAT TGAAAGGTGT CAAAGGAGCA AGAGGACCCC CGGGATATGA AGGAGAAACG
    GATATTATTT CCAAGAAGGG GGAAACTGGA GAACCTGGAC CTTCTGGGGA TGGTGGATTC
    CCAGGAGAAA ATGGTGATAA AGGTCATCCT GGGATGCAAG GAAGGAGAGG AGAGCCAGGA
    AGCCATGGAC CACCTGGATT TCACCCTGGG GAGCCCGGAA GAAATGGACA GCCAGGGCTT
    CCTGGACCCC CAGGCCCTCC AGGCTCACCT GGGCTGAGAG GAAACATTGG TTTTCCAGGA
    TTTCCGGGTG ACCAGGGTGA GCCAGGTTCT CCAGGGTGCC CGGGACCTTC AGGAATGGAT
    GGTACAAGAG GACCTAAAGG AGACAAAGGG GAATCTGCAA GTCAGTTTGG CCTGCCTGGT
    CCAAAGGGTG AGCCGGGTGG CCCTGGATAT CCAGGACGTT TGGGAGCATC TGGAGAGCAG
    GGCTTGCCCG GTGCTCAAGG TCCTGGAGGA CCACCCGGAA GGCCAGGACC TCCTGGCTTG
    TCTGGACCAA CAGGATGTCC AGGTAGTCCA GGGGGGCCTG GGCTGCAGGG ACCCCCAGGA
    GAAGTGGGGG ATCCTGGACC AAGAGGCGTC CTGGGGGACC CAGGAGCACC AGGTCTTCCA
    GGAATAAAAG GTCCCTCTGG GTCGCCCGGC CTGAACGGCC TGCATGGTTT GAAGGGTCAG
    AAAGGCAGCA AAGGCGCTTC AGGTTCGCAT GAAATGGGCC CCCCAGGTCC AGTGGGAATG
    CCCGGCCCTA AAGGGGAGAC AGGAGACCCC GGGAACCCAG GAATTTCTCC TCCGGGACTT
    TTTGGAGAAA GAGGTCCCCT GGGTCCCCCA GGACCCCCAG GGAGAGCTGG ACCACCTGGT
    CCTCCAGGTG CCCCAGGAAA AGCTCTTAAG GGTGACATTC CAGACCCGGG TCTGCCTGGA
    GATCAGGGGC CTCCTGGCCC CGACGGTCCA AGAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGC ACACAGGCGC TGGGGACCAA GGAGGCGGGC AGGCCCTCAT
    GTCCCCAGGC AGCTGCCTAG AGGATTTCAG AGCCGCACCG TTCCTGGAAT GCCAAGGCCG
    GCAGGGAACT TGCCACTTTT TTGCCAACAA GTATAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa88012
    Clone ID Related Accession (Same CDS sequence) XM_015459732.1
    Accession Version XM_015459732.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5130bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product collagen alpha-4(IV) chain
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103850.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 3% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGTG
    AGTCATGTGA AATTTAGCAT GGGTGGTGGG AGGGGAAAAT GGGAAATTAC TAATCAAAGG
    GCGTTTCTGG GTGGTCGAGA AGAGCTGTGT GTGGAACACC GGTCAACCTC TGCCCTTCCT
    GGCACAGATG GATCCCTGAT GATGGCTGTG AGGCGCTGGC GACGTTCTCT AGCTAGGAAT
    TTGGGAGCGT GGCTTGTGCG TAACCGGGAA GAGCCCACTG GGATCCCAGG GGAGAAAGGG
    ATGAAAGGTG ACAGTGGCCG TCCTGGAGCA GCAGGTGACG TAGGTGACAA GGGTCCAACC
    GGAGTTCCTG GATTTCCAGG TCTGGATGGG ATACCTGGGC ACCCAGGGCC TCCTGGATCC
    AGAGGCAGAC CTGGCATAGA TGGCTACAAT GGCTCAAGAG GTGATCCAGG GATTCCAGGA
    GAAAGAGGAG CTCTTGGCCC ACGAGGGCCC CCAGGCCTTC CTGGAGAAAA AGGAGAAAAA
    GGAAATTCAG TGTTCATTTT AGGTGCCACT AAAGGTATGC AGGGCGACAG AGGGGACCCA
    GGACCTCCCG GCTCACCAGG ATCAAGGGGA GTGAGAGGCC CAGCAGGCCC AGTGGGGTAC
    CCAGGAGAGC CCGGGTTAGC AGGACCTCCA GGCCCCCCTG GGAGTCCAGG CTTAAAGGGT
    AATCCTGGCG TGGGAGTAAA GGGGCAAATG GGAGACCCGG GTGAGGTTGG CCAACAAGGT
    TCTCCGGGGC CCACTCTGTT GGTGCAGCCA CCTGATTCTG GTCTCTATAA AGGAGAAAAG
    GGTATTAAAG GAATGCCTGG AATGACTGGA CTTCCTGGAC CACCGGGACC CAGGGGAGAG
    CCTGGTATTG GGAGAAAAGG AGAGAAAGGG ATTCCTGGGT TTCCAGGACC TCGGGGGGAC
    CCTGGTTCCT ATGGATCTCC AGGTTTTCCA GGATTAAAGG GGGAACCAGG ACTGTTTGGA
    GATCCTGGGT CATTTGGATT TCTTGGCCCA AAGGGAGATC CTGGAGACCG AGGGCACCCA
    GGACCACCAG GGGTTTTGGT AACTCCATCT CTTCCACTCA AAGGCCCTCC AGGGGATCCA
    GGGCGCCCTG GCCGCTATGG AGAAACAGGG GCTGTTGGAC CACGTGGTCC TCCTGGTGCC
    CCAGGTAGAC CAGGGGAAGC CTGTCCAGGC ATGACGGGAC CTCCTGGGCC ACGAGGATTT
    CCTGGTCACC CAGGATTTCC AGGGGCAGCT GGGGTTCCTG GAAGAGCTGA TTCTGCTCCA
    GGCAAACCAG GCAACCAGGG ACCAACTGGG CTGCCTGGAG TGCCGGGACT GCCGGGACCT
    CCGGGATCAG ATGCTGTATA TTGTACTGTT GGGCAACCCG GACCACAAGG AACAAAAGGC
    AAAGTGGGTC CTCCAGGAAG ACGAGGCTCC AAAGGAGAAA AAGGGAGCTC AGGGCTCTGT
    GCCTGCCAGC CTGGTCCCAT GGGCCCATCA GGCCCTCCAG GGCCTCCTGG GAGGCAAGGG
    AGTAAGGGAG ACTTGGGGCT CCCTGGGTGG CTTGGAGAAA AAGGTTCCCC AGGCCCTCCT
    GGTGCCAAAG GATCTCCAGG GCCACCGGGA AAACCTGGTG CCTCAGGACT ACCTGGCAGC
    AAAGGAGAAA AAGGTGACAT GGTTGTATCA AGAGTGAAAG GACACAAAGG AGAAAGAGGG
    CCTGATGGGC CCCCTGGACT TCCAGGGCAG CAGGGACAAC ACGGTCGAGA TGGATATGCT
    GGAGAAAAGG GGGACACAGG GCCGCCTGGT GATCACGAGG ATGCAGCCCC AGGTGATAAA
    GGGCCTCCTG GACCGCCAGG GCCCCCTGGC AGAGCGGGAC CTAGGGGGCC CCCAGGCCTG
    GGATTTCCTG GCCCTCCAGG AGACAGAGGG CAACCAGGAG CTCCAGGCTA CCCAGGCAAC
    AGGGGTCCTG ATGGTCGGAA GGGTCGGAAA GGTGATACAA TTCCTTGTAA CGTAACATAC
    CCTGGGAGGC CAGGCCCTCC AGGTTTTGAT GGTCCTCCAG GTCCAAAGGG ACTTCCAGGT
    CATCGGGGTG CTCCTGGGCT GAGGTGTTTG GATGGACAGA AGGGTCAACG TGGCAAACCA
    GGAATCTCAG AAAGACCTGG TCCACCTGGT TTTCGTGGTG ATATAGGAGA ACCAGGTTTG
    GGAGGTGAAA AGGGGTCCTC GCCTGTTGGG CCCCCAGGCA CTCCAGGTTC ACCTGGAGTG
    AATGGTCAGA AAGGACCCCC AGGAGATCCT GCTATCGGCT ACCCAGGACT CCCGGGAAAG
    AGCGGTCTTC CAGGAGCACC AGGGTCCAAA GGACTCAGAG GCGATGCCGG GCGTCCAGGG
    ACCGCAGGGC CAGCTGGCAT GCCCGGATTT CCGGGTCTCC AAGGTCCCAA AGGCCGAGAG
    GGAAATGCTG GGTTTCCAGG GATCCCAGGC CCACCTGGTC ACTCCTGTGA AAGAGGTGCT
    CCAGGGATAC CAGGGCAACC GGGGCTCCCT GGGGCTCCAG GCAGTCCAGG TGCCCCAGGT
    TGGAAAGGAC AGCGAGGGGA CATGGGACCT CTGGGACCTG CTGGAATGAA GGGCCTTCCT
    GGAGTCCCAG GATGGCCGGG GGCAGATGGA ACCCCAGGTC TTCCAGGGGT CCCAGGCCCC
    ACTGGAGACG ATGGGCTACC TGGTCTTCCA GGCCCAAAGG GACCCCAGGG GCTGCCTGGC
    TTCCTGGGTT TTCCAGGAGA GAGAGGAAAA CCTGGTCAGG ATGGACACCC TGGCAGAAAG
    GGAGAACATG GAGAGAAGGG TTGGCCTGGC TTCCTGGGAG ACCGAGGATT GAAAGGTGTC
    AAAGGAGCAA GAGGACCCCC GGGATATGAA GGAGAAACGG ATATTATTTC CAAGAAGGGG
    GAAACTGGAG AACCTGGACC TTCTGGGGAT GGTGGATTCC CAGGAGAAAA TGGTGATAAA
    GGTCATCCTG GGATGCAAGG AAGGAGAGGA GAGCCAGGAA GCCATGGACC ACCTGGATTT
    CACCCTGGGG AGCCCGGAAG AAATGGACAG CCAGGGCTTC CTGGACCCCC AGGCCCTCCA
    GGCTCACCTG GGCTGAGAGG AAACATTGGT TTTCCAGGAT TTCCGGGTGA CCAGGGTGAG
    CCAGGTTCTC CAGGGTGCCC GGGACCTTCA GGAATGGATG GTACAAGAGG ACCTAAAGGA
    GACAAAGGGG AATCTGCAAG TCAGTTTGGC CTGCCTGGTC CAAAGGGTGA GCCGGGTGGC
    CCTGGATATC CAGGACGTTT GGGAGCATCT GGAGAGCAGG GCTTGCCCGG TGCTCAAGGT
    CCTGGAGGAC CACCCGGAAG GCCAGGACCT CCTGGCTTGT CTGGACCAAC AGGATGTCCA
    GGTAGTCCAG GGGGGCCTGG GCTGCAGGGA CCCCCAGGAG AAGTGGGGGA TCCTGGACCA
    AGAGGCGTCC TGGGGGACCC AGGAGCACCA GGTCTTCCAG GAATAAAAGG TAAGTCAGAA
    GGAACCCTGA CCCCTCAGAA AGTTCTAGCC AATGATGCTC CCACAAGTTC AGAGTGCCTC
    TCTTGCACCC CAGGGACATG CTCTGGGGTC CCCCTTTTTT CCGGGATGCC TGTGGACACC
    TCCTTTTCTT TCTTACTCAG GCAGCACTGG CCTGGTCTCC TGACCGGCTC TACCAAAATG
    ATGCTCAAAT CTTTTTGCCT ACTCGTGTGG TCAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015315218.1
    CDS269..5398
    Translation

    Target ORF information:

    RefSeq Version XM_015459732.1
    Organism Bos taurus(cattle)
    Definition Bos taurus collagen type IV alpha 4 (COL4A4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015459732.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGGCACTGG TAAGGCGTCC TTTCAAGTGC AACAAGCTCT TGGCCACCAA TTCCTGGTCA 
    CTCATACTTA TTTTGTTTTC TGTACAATAT GCATATGGGG GTGGAAAGAA GTTTATTGGT
    CTCTGTGGAG GAAGAGATTG CTCTCTTTGC CAGTGCTTTC CTGAAAAGGG ATCTCGGGTG
    AGTCATGTGA AATTTAGCAT GGGTGGTGGG AGGGGAAAAT GGGAAATTAC TAATCAAAGG
    GCGTTTCTGG GTGGTCGAGA AGAGCTGTGT GTGGAACACC GGTCAACCTC TGCCCTTCCT
    GGCACAGATG GATCCCTGAT GATGGCTGTG AGGCGCTGGC GACGTTCTCT AGCTAGGAAT
    TTGGGAGCGT GGCTTGTGCG TAACCGGGAA GAGCCCACTG GGATCCCAGG GGAGAAAGGG
    ATGAAAGGTG ACAGTGGCCG TCCTGGAGCA GCAGGTGACG TAGGTGACAA GGGTCCAACC
    GGAGTTCCTG GATTTCCAGG TCTGGATGGG ATACCTGGGC ACCCAGGGCC TCCTGGATCC
    AGAGGCAGAC CTGGCATAGA TGGCTACAAT GGCTCAAGAG GTGATCCAGG GATTCCAGGA
    GAAAGAGGAG CTCTTGGCCC ACGAGGGCCC CCAGGCCTTC CTGGAGAAAA AGGAGAAAAA
    GGAAATTCAG TGTTCATTTT AGGTGCCACT AAAGGTATGC AGGGCGACAG AGGGGACCCA
    GGACCTCCCG GCTCACCAGG ATCAAGGGGA GTGAGAGGCC CAGCAGGCCC AGTGGGGTAC
    CCAGGAGAGC CCGGGTTAGC AGGACCTCCA GGCCCCCCTG GGAGTCCAGG CTTAAAGGGT
    AATCCTGGCG TGGGAGTAAA GGGGCAAATG GGAGACCCGG GTGAGGTTGG CCAACAAGGT
    TCTCCGGGGC CCACTCTGTT GGTGCAGCCA CCTGATTCTG GTCTCTATAA AGGAGAAAAG
    GGTATTAAAG GAATGCCTGG AATGACTGGA CTTCCTGGAC CACCGGGACC CAGGGGAGAG
    CCTGGTATTG GGAGAAAAGG AGAGAAAGGG ATTCCTGGGT TTCCAGGACC TCGGGGGGAC
    CCTGGTTCCT ATGGATCTCC AGGTTTTCCA GGATTAAAGG GGGAACCAGG ACTGTTTGGA
    GATCCTGGGT CATTTGGATT TCTTGGCCCA AAGGGAGATC CTGGAGACCG AGGGCACCCA
    GGACCACCAG GGGTTTTGGT AACTCCATCT CTTCCACTCA AAGGCCCTCC AGGGGATCCA
    GGGCGCCCTG GCCGCTATGG AGAAACAGGG GCTGTTGGAC CACGTGGTCC TCCTGGTGCC
    CCAGGTAGAC CAGGGGAAGC CTGTCCAGGC ATGACGGGAC CTCCTGGGCC ACGAGGATTT
    CCTGGTCACC CAGGATTTCC AGGGGCAGCT GGGGTTCCTG GAAGAGCTGA TTCTGCTCCA
    GGCAAACCAG GCAACCAGGG ACCAACTGGG CTGCCTGGAG TGCCGGGACT GCCGGGACCT
    CCGGGATCAG ATGCTGTATA TTGTACTGTT GGGCAACCCG GACCACAAGG AACAAAAGGC
    AAAGTGGGTC CTCCAGGAAG ACGAGGCTCC AAAGGAGAAA AAGGGAGCTC AGGGCTCTGT
    GCCTGCCAGC CTGGTCCCAT GGGCCCATCA GGCCCTCCAG GGCCTCCTGG GAGGCAAGGG
    AGTAAGGGAG ACTTGGGGCT CCCTGGGTGG CTTGGAGAAA AAGGTTCCCC AGGCCCTCCT
    GGTGCCAAAG GATCTCCAGG GCCACCGGGA AAACCTGGTG CCTCAGGACT ACCTGGCAGC
    AAAGGAGAAA AAGGTGACAT GGTTGTATCA AGAGTGAAAG GACACAAAGG AGAAAGAGGG
    CCTGATGGGC CCCCTGGACT TCCAGGGCAG CAGGGACAAC ACGGTCGAGA TGGATATGCT
    GGAGAAAAGG GGGACACAGG GCCGCCTGGT GATCACGAGG ATGCAGCCCC AGGTGATAAA
    GGGCCTCCTG GACCGCCAGG GCCCCCTGGC AGAGCGGGAC CTAGGGGGCC CCCAGGCCTG
    GGATTTCCTG GCCCTCCAGG AGACAGAGGG CAACCAGGAG CTCCAGGCTA CCCAGGCAAC
    AGGGGTCCTG ATGGTCGGAA GGGTCGGAAA GGTGATACAA TTCCTTGTAA CGTAACATAC
    CCTGGGAGGC CAGGCCCTCC AGGTTTTGAT GGTCCTCCAG GTCCAAAGGG ACTTCCAGGT
    CATCGGGGTG CTCCTGGGCT GAGGTGTTTG GATGGACAGA AGGGTCAACG TGGCAAACCA
    GGAATCTCAG AAAGACCTGG TCCACCTGGT TTTCGTGGTG ATATAGGAGA ACCAGGTTTG
    GGAGGTGAAA AGGGGTCCTC GCCTGTTGGG CCCCCAGGCA CTCCAGGTTC ACCTGGAGTG
    AATGGTCAGA AAGGACCCCC AGGAGATCCT GCTATCGGCT ACCCAGGACT CCCGGGAAAG
    AGCGGTCTTC CAGGAGCACC AGGGTCCAAA GGACTCAGAG GCGATGCCGG GCGTCCAGGG
    ACCGCAGGGC CAGCTGGCAT GCCCGGATTT CCGGGTCTCC AAGGTCCCAA AGGCCGAGAG
    GGAAATGCTG GGTTTCCAGG GATCCCAGGC CCACCTGGTC ACTCCTGTGA AAGAGGTGCT
    CCAGGGATAC CAGGGCAACC GGGGCTCCCT GGGGCTCCAG GCAGTCCAGG TGCCCCAGGT
    TGGAAAGGAC AGCGAGGGGA CATGGGACCT CTGGGACCTG CTGGAATGAA GGGCCTTCCT
    GGAGTCCCAG GATGGCCGGG GGCAGATGGA ACCCCAGGTC TTCCAGGGGT CCCAGGCCCC
    ACTGGAGACG ATGGGCTACC TGGTCTTCCA GGCCCAAAGG GACCCCAGGG GCTGCCTGGC
    TTCCTGGGTT TTCCAGGAGA GAGAGGAAAA CCTGGTCAGG ATGGACACCC TGGCAGAAAG
    GGAGAACATG GAGAGAAGGG TTGGCCTGGC TTCCTGGGAG ACCGAGGATT GAAAGGTGTC
    AAAGGAGCAA GAGGACCCCC GGGATATGAA GGAGAAACGG ATATTATTTC CAAGAAGGGG
    GAAACTGGAG AACCTGGACC TTCTGGGGAT GGTGGATTCC CAGGAGAAAA TGGTGATAAA
    GGTCATCCTG GGATGCAAGG AAGGAGAGGA GAGCCAGGAA GCCATGGACC ACCTGGATTT
    CACCCTGGGG AGCCCGGAAG AAATGGACAG CCAGGGCTTC CTGGACCCCC AGGCCCTCCA
    GGCTCACCTG GGCTGAGAGG AAACATTGGT TTTCCAGGAT TTCCGGGTGA CCAGGGTGAG
    CCAGGTTCTC CAGGGTGCCC GGGACCTTCA GGAATGGATG GTACAAGAGG ACCTAAAGGA
    GACAAAGGGG AATCTGCAAG TCAGTTTGGC CTGCCTGGTC CAAAGGGTGA GCCGGGTGGC
    CCTGGATATC CAGGACGTTT GGGAGCATCT GGAGAGCAGG GCTTGCCCGG TGCTCAAGGT
    CCTGGAGGAC CACCCGGAAG GCCAGGACCT CCTGGCTTGT CTGGACCAAC AGGATGTCCA
    GGTAGTCCAG GGGGGCCTGG GCTGCAGGGA CCCCCAGGAG AAGTGGGGGA TCCTGGACCA
    AGAGGCGTCC TGGGGGACCC AGGAGCACCA GGTCTTCCAG GAATAAAAGG TAAGTCAGAA
    GGAACCCTGA CCCCTCAGAA AGTTCTAGCC AATGATGCTC CCACAAGTTC AGAGTGCCTC
    TCTTGCACCC CAGGGACATG CTCTGGGGTC CCCCTTTTTT CCGGGATGCC TGTGGACACC
    TCCTTTTCTT TCTTACTCAG GCAGCACTGG CCTGGTCTCC TGACCGGCTC TACCAAAATG
    ATGCTCAAAT CTTTTTGCCT ACTCGTGTGG TCAGGAGTAC CCGGGCCTCC AGGACCACCT
    GGGAGTGTCG ACCTTCTGAA AGGAGAACCA GGAGACTGTG GTCTGCCAGG GCCTCCAGGT
    CTCCCGGGCC CACCAGGCCC TCCAGGACAC AAGGGCTTCC CAGGATGTGA TGGAAAACAT
    GGCCAGAAAG GACCAATGGG ATTCCCGGGG CCGCAGGGGC CACCTGGAAG TCCTGGGCCA
    CCTGGAGACA AAGGTTTACC TGGACCTCCA GGCAGACGAG GGCCTTTGGG TCCTCCGGGT
    TCCAGAGGTG AGCCTGGGCC CCCAGCAGAC TTGGATGCCT GCCCCCGAAT CCCAGGGCTT
    CCTGGGGTAC CAGGCCCCAG AGGACCGGAA GGAACCATGG GGCTTCCTGG CATGAGAGGC
    CCGCCGGGAC CAGGGTGCAA AGGAGAGCCT GGGCTGGATG GCAGGAGGGG TGAGGATGGC
    CTCCCAGGGT CCCCTGGGCC TCCGGGACAT AAAGGTGACA TGGGAGAAGC AGGCTGTCCA
    GGAGCACCAG GCCCTCCTGG GCCCATGGGG GATCCTGGGC CCATAGGGTT TGGCCCTGGA
    TACCTCAGTG GCTTCCTCCT GGTTCTCCAC AGTCAGACGG ATGGAGAACC CACCTGCCCC
    ATGGGCATGC CCAGGCTCTG GACTGGGTAT AGTCTGCTAT ACCTGGAAGG ACAGGAGAGA
    GCTCACAATC AAGACCTCGG TCTGGCGGGC TCCTGCCTCC CCATCTTTAG CACGCTGCCC
    TTCGCCTACT GCAACATCCA CCAGGTGTGC CACTATGCCC GGAGGAACGA CAGGTCCTAC
    TGGCTGGCGA GCACAGCGCC TCTGCCCATG ACTCCGCTCT CGGAGGACGA GATCCGCCCG
    TACATCAGCC GCTGCGCAGT GTGCGAGGCC CCCGCCCAGG CCGTGGCGGT GCACAGCCAA
    GACCAGTCCA TCCCCCCATG TCCGCGGGCC TGGAGGAGCC TCTGGATCGG GTACTCCTTC
    CTCATGCACA CAGGCGCTGG GGACCAAGGA GGCGGGCAGG CCCTCATGTC CCCAGGCAGC
    TGCCTAGAGG ATTTCAGAGC CGCACCGTTC CTGGAATGCC AAGGCCGGCA GGGAACTTGC
    CACTTTTTTG CCAACAAGTA TAGTTTCTGG CTGACAACAG TGAGACCTGA CTTGCAGTTT
    TCCTCTGCAC CATTACCGGA CACCTTGAAA GAAAGCCATG CTCAACGCCA GAAAATCAGC
    AGGTGCCAGG TCTGCGTGAA GCATAGCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.