ORF » Species Summary » Bos taurus » PRF1 cDNA ORF clone

PRF1 cDNA ORF clone, Bos taurus(cattle)

The following PRF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa34179 XM_005226334.4
Latest version!		 Bos taurus perforin 1 (PRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OBa37932 NM_001143735.1
Latest version!		 Bos taurus perforin 1 (PRF1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
OBa34179 XM_005226334.3 Bos taurus perforin 1 (pore forming protein) (PRF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    PRF1 ( XM_005226334.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226391.1 Bos taurus perforin 1 (pore forming protein) (PRF1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa34179
    Clone ID Related Accession (Same CDS sequence) XM_005226334.3 , XM_005226334.4
    Accession Version XM_005226334.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product perforin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104625.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005226334.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGACTCCA GCTCCATGGC TGCCCGAGTG CTCCTCCTTG GCATCCTCCT GCTTCTGCCC 
    ACACCTGCCC CTGCCCCCTG CTACACAGCC GCGCGCTCTG AGTGCCAGCG CGCCCTCAAG 
    TTTGTGCCGG GCTCCTGGCT GGCAGGGGAG GGCGTGGATG TGACCAGCCT CCAGCGCTCA 
    GGCTCGTTCC CAGTGGACAC ACAGCGTTTC CTGCGGCCCG ACGGCACTTG CACCCTCTGC 
    CGCAATGCCC TGCAGAAGGA TGTCCTCCAG CGCCTGCCCC TGGCAATCAC CGACTGGCGT 
    GCCCACGGAG CGGGCTGCAA GCGCAGGGTG GTCAAGCTAG AGGGCCGCTC CACCGAGGAT 
    GTGGCTGGGG AGGCGGCCAA CAGGATCCGC AACGACTGGC AGGTGGGGCT GGACGTGTCT 
    CCCAAGCCAA ATGCTAATGT CCGTGTGACA GTGGCGGGCT CCCACTCCGA GGATGCCAAC 
    TTCGCCGCCC AGAAGACTCA CCAGGACAAC TACCGCTTCA GCATGGACTT AGTGGAGTGT 
    CGCTTTTACA GTTTTCACCT GGTGCACACT CCCCCAGTAC ACCCTGAGTT CAAGAGGGCC 
    CTCAAGACAC TGCCCCCCCA CTTCAACACC TCCACCAAGC CCGACTACCA CAGGCTCATC 
    TCCAGCTACG GAACCCACTT CATCCGGTCC ATGGAGCTGG GCGGCCGCAT CTCGGCCCTC 
    ACCGCCCTGC GCACCTGCGA GCTGGCCCTG GAGGGGCTCA CAGCCAGCGA GGTCGAGGAC 
    TGCCTGGCTG TCGAGGCTGA GGTCAGCATA AGCGACAGGG CCAGTGCCTC GCCATCGTTC 
    AAGGCATGTG AGGAGAAGAA GAAGAACCAC AAGGTGGGGA CCTCCTTCCA CCAGGCCTAC 
    CGGGAGCGCC ATTCCAATGT CGATGGTGGC CACCACTCAA CCATGCATGA CCTGCTCTTC 
    GGGAGCCAGG CTGGGCCCGA GCAGTTCTCA GCCTGGGTGG CCTCACTGCA GGACAGCCCT 
    GGCCTGGTGG ACTACACGCT GGAGCCTCTG CACATGCTTG TGGAGAGCCA GGACCCGCGG 
    CGGGAGGCCC TCAGGCAGGC CGTGAGCAAG TACGTGACTG ACAGGGCACG CTGGAGGGAC 
    TGCAACCGCC CGTGCCCCCC GGGGCAACAC AAGAACCCGA AGAACCCATG CCAGTGCATG 
    TGTCCTGGTT CAGCAGCCAC CACCCAGGAC TGCTGTCCCC GGCAGAAGGG ACTGGCCCAC 
    CTGGAGGTCA TGAACTTCAA GGCATCAGGT CTGTGGGGAG ACTGGATCAC TGCCACGGAC 
    GCCTATCTGA AGGTCTTCTT CGCCGGCCAG GAGCAGAGGA CCGCCACAGT ATGGAACGAT 
    AACAACCCCA GGTGGATGAC GCGGCTGGAC TTCGGGGATG TGCTCCTGGC CACCGGGGGC 
    CCCCTGAGGG TGCAGGTCTG GGATGCAGAC TTTGGCTGGG ACGATGACCT TCTTGGCACT 
    TGTGACCGCA CCCCAAAGTC TGGCTCACAT GAGGTGTCAT GCCCCATGGA CCACGGTCTC 
    CTGAAATTCT CCTACCAGGT CAAATGCTTG CCTCACCTGA CGGGGGAGAG GTGCCTGCAG 
    TATGCCCCCC AAGGGCTTCT GGGGAATCCT CCAGGAAACC GGAGTGGGCC AGTGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226391.1
    CDS121..1800
    Translation

    Target ORF information:

    RefSeq Version XM_005226334.3
    Organism Bos taurus(cattle)
    Definition Bos taurus perforin 1 (pore forming protein) (PRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005226334.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGACTCCA GCTCCATGGC TGCCCGAGTG CTCCTCCTTG GCATCCTCCT GCTTCTGCCC 
    ACACCTGCCC CTGCCCCCTG CTACACAGCC GCGCGCTCTG AGTGCCAGCG CGCCCTCAAG 
    TTTGTGCCGG GCTCCTGGCT GGCAGGGGAG GGCGTGGATG TGACCAGCCT CCAGCGCTCA 
    GGCTCGTTCC CAGTGGACAC ACAGCGTTTC CTGCGGCCCG ACGGCACTTG CACCCTCTGC 
    CGCAATGCCC TGCAGAAGGA TGTCCTCCAG CGCCTGCCCC TGGCAATCAC CGACTGGCGT 
    GCCCACGGAG CGGGCTGCAA GCGCAGGGTG GTCAAGCTAG AGGGCCGCTC CACCGAGGAT 
    GTGGCTGGGG AGGCGGCCAA CAGGATCCGC AACGACTGGC AGGTGGGGCT GGACGTGTCT 
    CCCAAGCCAA ATGCTAATGT CCGTGTGACA GTGGCGGGCT CCCACTCCGA GGATGCCAAC 
    TTCGCCGCCC AGAAGACTCA CCAGGACAAC TACCGCTTCA GCATGGACTT AGTGGAGTGT 
    CGCTTTTACA GTTTTCACCT GGTGCACACT CCCCCAGTAC ACCCTGAGTT CAAGAGGGCC 
    CTCAAGACAC TGCCCCCCCA CTTCAACACC TCCACCAAGC CCGACTACCA CAGGCTCATC 
    TCCAGCTACG GAACCCACTT CATCCGGTCC ATGGAGCTGG GCGGCCGCAT CTCGGCCCTC 
    ACCGCCCTGC GCACCTGCGA GCTGGCCCTG GAGGGGCTCA CAGCCAGCGA GGTCGAGGAC 
    TGCCTGGCTG TCGAGGCTGA GGTCAGCATA AGCGACAGGG CCAGTGCCTC GCCATCGTTC 
    AAGGCATGTG AGGAGAAGAA GAAGAACCAC AAGGTGGGGA CCTCCTTCCA CCAGGCCTAC 
    CGGGAGCGCC ATTCCAATGT CGATGGTGGC CACCACTCAA CCATGCATGA CCTGCTCTTC 
    GGGAGCCAGG CTGGGCCCGA GCAGTTCTCA GCCTGGGTGG CCTCACTGCA GGACAGCCCT 
    GGCCTGGTGG ACTACACGCT GGAGCCTCTG CACATGCTTG TGGAGAGCCA GGACCCGCGG 
    CGGGAGGCCC TCAGGCAGGC CGTGAGCAAG TACGTGACTG ACAGGGCACG CTGGAGGGAC 
    TGCAACCGCC CGTGCCCCCC GGGGCAACAC AAGAACCCGA AGAACCCATG CCAGTGCATG 
    TGTCCTGGTT CAGCAGCCAC CACCCAGGAC TGCTGTCCCC GGCAGAAGGG ACTGGCCCAC 
    CTGGAGGTCA TGAACTTCAA GGCATCAGGT CTGTGGGGAG ACTGGATCAC TGCCACGGAC 
    GCCTATCTGA AGGTCTTCTT CGCCGGCCAG GAGCAGAGGA CCGCCACAGT ATGGAACGAT 
    AACAACCCCA GGTGGATGAC GCGGCTGGAC TTCGGGGATG TGCTCCTGGC CACCGGGGGC 
    CCCCTGAGGG TGCAGGTCTG GGATGCAGAC TTTGGCTGGG ACGATGACCT TCTTGGCACT 
    TGTGACCGCA CCCCAAAGTC TGGCTCACAT GAGGTGTCAT GCCCCATGGA CCACGGTCTC 
    CTGAAATTCT CCTACCAGGT CAAATGCTTG CCTCACCTGA CGGGGGAGAG GTGCCTGCAG 
    TATGCCCCCC AAGGGCTTCT GGGGAATCCT CCAGGAAACC GGAGTGGGCC AGTGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    PRF1 ( XM_005226334.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005226391.1 Bos taurus perforin 1 (PRF1), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID OBa34179
    Clone ID Related Accession (Same CDS sequence) XM_005226334.3 , XM_005226334.4
    Accession Version XM_005226334.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1680bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product perforin-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037355.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005226334.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGACTCCA GCTCCATGGC TGCCCGAGTG CTCCTCCTTG GCATCCTCCT GCTTCTGCCC 
    ACACCTGCCC CTGCCCCCTG CTACACAGCC GCGCGCTCTG AGTGCCAGCG CGCCCTCAAG 
    TTTGTGCCGG GCTCCTGGCT GGCAGGGGAG GGCGTGGATG TGACCAGCCT CCAGCGCTCA 
    GGCTCGTTCC CAGTGGACAC ACAGCGTTTC CTGCGGCCCG ACGGCACTTG CACCCTCTGC 
    CGCAATGCCC TGCAGAAGGA TGTCCTCCAG CGCCTGCCCC TGGCAATCAC CGACTGGCGT 
    GCCCACGGAG CGGGCTGCAA GCGCAGGGTG GTCAAGCTAG AGGGCCGCTC CACCGAGGAT 
    GTGGCTGGGG AGGCGGCCAA CAGGATCCGC AACGACTGGC AGGTGGGGCT GGACGTGTCT 
    CCCAAGCCAA ATGCTAATGT CCGTGTGACA GTGGCGGGCT CCCACTCCGA GGATGCCAAC 
    TTCGCCGCCC AGAAGACTCA CCAGGACAAC TACCGCTTCA GCATGGACTT AGTGGAGTGT 
    CGCTTTTACA GTTTTCACCT GGTGCACACT CCCCCAGTAC ACCCTGAGTT CAAGAGGGCC 
    CTCAAGACAC TGCCCCCCCA CTTCAACACC TCCACCAAGC CCGACTACCA CAGGCTCATC 
    TCCAGCTACG GAACCCACTT CATCCGGTCC ATGGAGCTGG GCGGCCGCAT CTCGGCCCTC 
    ACCGCCCTGC GCACCTGCGA GCTGGCCCTG GAGGGGCTCA CAGCCAGCGA GGTCGAGGAC 
    TGCCTGGCTG TCGAGGCTGA GGTCAGCATA AGCGACAGGG CCAGTGCCTC GCCATCGTTC 
    AAGGCATGTG AGGAGAAGAA GAAGAACCAC AAGGTGGGGA CCTCCTTCCA CCAGGCCTAC 
    CGGGAGCGCC ATTCCAATGT CGATGGTGGC CACCACTCAA CCATGCATGA CCTGCTCTTC 
    GGGAGCCAGG CTGGGCCCGA GCAGTTCTCA GCCTGGGTGG CCTCACTGCA GGACAGCCCT 
    GGCCTGGTGG ACTACACGCT GGAGCCTCTG CACATGCTTG TGGAGAGCCA GGACCCGCGG 
    CGGGAGGCCC TCAGGCAGGC CGTGAGCAAG TACGTGACTG ACAGGGCACG CTGGAGGGAC 
    TGCAACCGCC CGTGCCCCCC GGGGCAACAC AAGAACCCGA AGAACCCATG CCAGTGCATG 
    TGTCCTGGTT CAGCAGCCAC CACCCAGGAC TGCTGTCCCC GGCAGAAGGG ACTGGCCCAC 
    CTGGAGGTCA TGAACTTCAA GGCATCAGGT CTGTGGGGAG ACTGGATCAC TGCCACGGAC 
    GCCTATCTGA AGGTCTTCTT CGCCGGCCAG GAGCAGAGGA CCGCCACAGT ATGGAACGAT 
    AACAACCCCA GGTGGATGAC GCGGCTGGAC TTCGGGGATG TGCTCCTGGC CACCGGGGGC 
    CCCCTGAGGG TGCAGGTCTG GGATGCAGAC TTTGGCTGGG ACGATGACCT TCTTGGCACT 
    TGTGACCGCA CCCCAAAGTC TGGCTCACAT GAGGTGTCAT GCCCCATGGA CCACGGTCTC 
    CTGAAATTCT CCTACCAGGT CAAATGCTTG CCTCACCTGA CGGGGGAGAG GTGCCTGCAG 
    TATGCCCCCC AAGGGCTTCT GGGGAATCCT CCAGGAAACC GGAGTGGGCC AGTGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005226391.1
    CDS148..1827
    Translation

    Target ORF information:

    RefSeq Version XM_005226334.4
    Organism Bos taurus(cattle)
    Definition Bos taurus perforin 1 (PRF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_005226334.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGACTCCA GCTCCATGGC TGCCCGAGTG CTCCTCCTTG GCATCCTCCT GCTTCTGCCC 
    ACACCTGCCC CTGCCCCCTG CTACACAGCC GCGCGCTCTG AGTGCCAGCG CGCCCTCAAG 
    TTTGTGCCGG GCTCCTGGCT GGCAGGGGAG GGCGTGGATG TGACCAGCCT CCAGCGCTCA 
    GGCTCGTTCC CAGTGGACAC ACAGCGTTTC CTGCGGCCCG ACGGCACTTG CACCCTCTGC 
    CGCAATGCCC TGCAGAAGGA TGTCCTCCAG CGCCTGCCCC TGGCAATCAC CGACTGGCGT 
    GCCCACGGAG CGGGCTGCAA GCGCAGGGTG GTCAAGCTAG AGGGCCGCTC CACCGAGGAT 
    GTGGCTGGGG AGGCGGCCAA CAGGATCCGC AACGACTGGC AGGTGGGGCT GGACGTGTCT 
    CCCAAGCCAA ATGCTAATGT CCGTGTGACA GTGGCGGGCT CCCACTCCGA GGATGCCAAC 
    TTCGCCGCCC AGAAGACTCA CCAGGACAAC TACCGCTTCA GCATGGACTT AGTGGAGTGT 
    CGCTTTTACA GTTTTCACCT GGTGCACACT CCCCCAGTAC ACCCTGAGTT CAAGAGGGCC 
    CTCAAGACAC TGCCCCCCCA CTTCAACACC TCCACCAAGC CCGACTACCA CAGGCTCATC 
    TCCAGCTACG GAACCCACTT CATCCGGTCC ATGGAGCTGG GCGGCCGCAT CTCGGCCCTC 
    ACCGCCCTGC GCACCTGCGA GCTGGCCCTG GAGGGGCTCA CAGCCAGCGA GGTCGAGGAC 
    TGCCTGGCTG TCGAGGCTGA GGTCAGCATA AGCGACAGGG CCAGTGCCTC GCCATCGTTC 
    AAGGCATGTG AGGAGAAGAA GAAGAACCAC AAGGTGGGGA CCTCCTTCCA CCAGGCCTAC 
    CGGGAGCGCC ATTCCAATGT CGATGGTGGC CACCACTCAA CCATGCATGA CCTGCTCTTC 
    GGGAGCCAGG CTGGGCCCGA GCAGTTCTCA GCCTGGGTGG CCTCACTGCA GGACAGCCCT 
    GGCCTGGTGG ACTACACGCT GGAGCCTCTG CACATGCTTG TGGAGAGCCA GGACCCGCGG 
    CGGGAGGCCC TCAGGCAGGC CGTGAGCAAG TACGTGACTG ACAGGGCACG CTGGAGGGAC 
    TGCAACCGCC CGTGCCCCCC GGGGCAACAC AAGAACCCGA AGAACCCATG CCAGTGCATG 
    TGTCCTGGTT CAGCAGCCAC CACCCAGGAC TGCTGTCCCC GGCAGAAGGG ACTGGCCCAC 
    CTGGAGGTCA TGAACTTCAA GGCATCAGGT CTGTGGGGAG ACTGGATCAC TGCCACGGAC 
    GCCTATCTGA AGGTCTTCTT CGCCGGCCAG GAGCAGAGGA CCGCCACAGT ATGGAACGAT 
    AACAACCCCA GGTGGATGAC GCGGCTGGAC TTCGGGGATG TGCTCCTGGC CACCGGGGGC 
    CCCCTGAGGG TGCAGGTCTG GGATGCAGAC TTTGGCTGGG ACGATGACCT TCTTGGCACT 
    TGTGACCGCA CCCCAAAGTC TGGCTCACAT GAGGTGTCAT GCCCCATGGA CCACGGTCTC 
    CTGAAATTCT CCTACCAGGT CAAATGCTTG CCTCACCTGA CGGGGGAGAG GTGCCTGCAG 
    TATGCCCCCC AAGGGCTTCT GGGGAATCCT CCAGGAAACC GGAGTGGGCC AGTGTGGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    PRF1 ( NM_001143735.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001137207.1 Bos taurus perforin 1 (PRF1), mRNA.
    Price & Availability↑
    CloneID OBa37932
    Clone ID Related Accession (Same CDS sequence) NM_001143735.1
    Accession Version NM_001143735.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1665bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-13
    Organism Bos taurus(cattle)
    Product perforin-1 precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from FJ176744.1. On Jan 6, 2009 this sequence version replaced XM_585583.3. ##Evidence-Data-START## Transcript exon combination :: FJ176744.1, AY313172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN05861797, SAMN05861817 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCAGCCC GTCTGCTCCT CCTTGGCATC CTCCTGCTTC TGCCCACACC TGCCCCTGCC 
    CCCTGCTACA CAGCCGCGCG CTCTGAGTGC CAGCGCGCCC TCAAGTTTGT GCCAGGCTCC 
    TGGCTGGCAG GGGAGGGCGT GGATGTGACC AGCCTCCAGC GCTCAGGCTC GTTCCCAGTG 
    GACACACAGC GTTTCCTGCG GCCCGACGGC ACTTGCACCC TCTGCCGCAA TGCCCTGCAG 
    AAGGATGTCC TCCAGCGCCT GCCCCTGGCA ATCACCGACT GGCGTGCCCA CGGAGCGGGC 
    TGCAAGCGCA GGGTGGTCAA GCTAGAGGGC CGCTCCACCG AGGATGTGGC TGGGGAGGCG 
    GCCAACGGGA TCCGCAACGA CTGGCAGGTG GGGCTGGACG TGTCTCCCAA GCCAAATGCT 
    AATGTCCGTG TGACAGTGGC GGGCTCCCAC TCCGAGGATG CCAACTTCGC CGCCCAGAAG 
    ACTCACCAGG ACAACTACCG CTTCGGCATG GACTTAGTGG AGTGTCGCTT TTACAGTTTT 
    CACCTGGTGC ACACTCCCCC AGTACACCCT GAGTTCAAGA GGGCCCTCAA GACACTGCCC 
    CCCCACTTCA ACACCTCCAC CAAGCCCGAC TACCACAGGC TCATCTCCAG CTACGGAACC 
    CACTTCATCC GGTCCATGGA GCTGGGCGGN CGCATCTCGG CCCTCACCGN CCTGCGNACC 
    TGCGAGCTGG CCCTGGAGGG GCTCACAGCC AGCGAGGTCG AGGACTGCCT GGCTGTCGAG 
    GCTGAGGTCA GCATAAGCGA CAGGGCCAGT GCCTCGCCAT CGTTCAAGGC ATGTGAGGAG 
    AAGAAGAAGA ACCACAAGGT GGGGACCTCC TTCCACCAGG CCTACCGGGA GCGCCATTCC 
    AATGTCGATG GTGGCCACCA CTCAACCATG CATGACCTGC TCTTCGGGAG CCAGGCTGGG 
    CCCGAGCAGT TCTCAGCCTG GGTGGCCTCA CTGCAGGACA GCCCTGGCCT GGTGGACTAC 
    ACGCTGGAGC CTCTGCACAT GCTTGTGGAG AGCCAGGACC CGCGGCGGGA GGCCCTCAGG 
    CAGGCCGTGA GCAAGTACGT GACTGACAGG GCACGCTGGA GGGACTGCAA CCGCCCGTGC 
    CCCCCGGGGC AACACAAGAA CCCGAAGAAC CCATGCCAGT GCATGTGTCC TGGTTCAGCA 
    GCCACCACCC AGGACTGCTG TCCCCGGCAG AAGGGACTGG CCCACCTGGA GGTCATGAAC 
    TTCAAGGCAT CAGGTCTGTG GGGAGACTGG ATCACTGCCA CGGACGCCTA TCTGAAGGTT 
    TTTTTCGCCG GCCAGGAGCA GAGGACCGCC ACAGTATGGA ACGATAACAA CCCCAGGTGG 
    ATGACGCGGC TGGACTTCGG GGATGTGCTC CTGGCCACCG GGGGCCCCCT GAGGGTGCAG 
    GTCTGGGATG CAGACTTTGG CTGGGACGAT GACCTTCTTG GCACTTGTGA CCGCACCCCA 
    AAGTCTGGCT CACATGAGGT GTCATGCCCC ATGGACCACG GTCTCTTGAA ATTCTCCTAC 
    CAGGTCAAAT GCTTGCCTCA CCTGACGGGG GAGAGGTGCC TGCAGTATGC CCCCCAAGGG 
    CTTCTGGGGA ATCCTCCAGG AAACCGGAGT GGGCCAGTGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001137207.1
    CDS1..1665
    Translation

    Target ORF information:

    RefSeq Version NM_001143735.1
    Organism Bos taurus(cattle)
    Definition Bos taurus perforin 1 (PRF1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001143735.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGCAGCCC GTCTGCTCCT CCTTGGCATC CTCCTGCTTC TGCCCACACC TGCCCCTGCC 
    CCCTGCTACA CAGCCGCGCG CTCTGAGTGC CAGCGCGCCC TCAAGTTTGT GCCAGGCTCC 
    TGGCTGGCAG GGGAGGGCGT GGATGTGACC AGCCTCCAGC GCTCAGGCTC GTTCCCAGTG 
    GACACACAGC GTTTCCTGCG GCCCGACGGC ACTTGCACCC TCTGCCGCAA TGCCCTGCAG 
    AAGGATGTCC TCCAGCGCCT GCCCCTGGCA ATCACCGACT GGCGTGCCCA CGGAGCGGGC 
    TGCAAGCGCA GGGTGGTCAA GCTAGAGGGC CGCTCCACCG AGGATGTGGC TGGGGAGGCG 
    GCCAACGGGA TCCGCAACGA CTGGCAGGTG GGGCTGGACG TGTCTCCCAA GCCAAATGCT 
    AATGTCCGTG TGACAGTGGC GGGCTCCCAC TCCGAGGATG CCAACTTCGC CGCCCAGAAG 
    ACTCACCAGG ACAACTACCG CTTCGGCATG GACTTAGTGG AGTGTCGCTT TTACAGTTTT 
    CACCTGGTGC ACACTCCCCC AGTACACCCT GAGTTCAAGA GGGCCCTCAA GACACTGCCC 
    CCCCACTTCA ACACCTCCAC CAAGCCCGAC TACCACAGGC TCATCTCCAG CTACGGAACC 
    CACTTCATCC GGTCCATGGA GCTGGGCGGN CGCATCTCGG CCCTCACCGN CCTGCGNACC 
    TGCGAGCTGG CCCTGGAGGG GCTCACAGCC AGCGAGGTCG AGGACTGCCT GGCTGTCGAG 
    GCTGAGGTCA GCATAAGCGA CAGGGCCAGT GCCTCGCCAT CGTTCAAGGC ATGTGAGGAG 
    AAGAAGAAGA ACCACAAGGT GGGGACCTCC TTCCACCAGG CCTACCGGGA GCGCCATTCC 
    AATGTCGATG GTGGCCACCA CTCAACCATG CATGACCTGC TCTTCGGGAG CCAGGCTGGG 
    CCCGAGCAGT TCTCAGCCTG GGTGGCCTCA CTGCAGGACA GCCCTGGCCT GGTGGACTAC 
    ACGCTGGAGC CTCTGCACAT GCTTGTGGAG AGCCAGGACC CGCGGCGGGA GGCCCTCAGG 
    CAGGCCGTGA GCAAGTACGT GACTGACAGG GCACGCTGGA GGGACTGCAA CCGCCCGTGC 
    CCCCCGGGGC AACACAAGAA CCCGAAGAAC CCATGCCAGT GCATGTGTCC TGGTTCAGCA 
    GCCACCACCC AGGACTGCTG TCCCCGGCAG AAGGGACTGG CCCACCTGGA GGTCATGAAC 
    TTCAAGGCAT CAGGTCTGTG GGGAGACTGG ATCACTGCCA CGGACGCCTA TCTGAAGGTT 
    TTTTTCGCCG GCCAGGAGCA GAGGACCGCC ACAGTATGGA ACGATAACAA CCCCAGGTGG 
    ATGACGCGGC TGGACTTCGG GGATGTGCTC CTGGCCACCG GGGGCCCCCT GAGGGTGCAG 
    GTCTGGGATG CAGACTTTGG CTGGGACGAT GACCTTCTTG GCACTTGTGA CCGCACCCCA 
    AAGTCTGGCT CACATGAGGT GTCATGCCCC ATGGACCACG GTCTCTTGAA ATTCTCCTAC 
    CAGGTCAAAT GCTTGCCTCA CCTGACGGGG GAGAGGTGCC TGCAGTATGC CCCCCAAGGG 
    CTTCTGGGGA ATCCTCCAGG AAACCGGAGT GGGCCAGTGT GGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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